Diagnosis and treatment of vascular disease

ABSTRACT

The present invention is based at least in part on the discovery of polymorphisms within the thrombospondin 1 (THBS1) and thrombospondin 4 (THBS4) genes. Accordingly, the invention provides nucleic acid molecules having a nucleotide sequence of an allelic variant of a THBS1 or THBS4 gene. The invention also provides methods for identifying specific alleles of polymorphic regions of a THBS1 or THBS4 gene, methods for determining whether a subject has or is at risk of developing a disease which is associated with a specific allele of a polymorphic region of a THBS1 or THBS4 gene, e.g., a vascular disease, based on detection of polymorphisms within the THBS1 or THBS4 gene, and kits for performing such methods. The invention further provides methods for identifying a subject who has, or is at risk for developing, a vascular disease or disorder as a candidate for a particular clinical course of therapy or a particular diagnostic evaluation. The invention further provides methods for selecting a clinical course of therapy or a diagnostic evaluation to treat a subject who is at risk for developing, a vascular disease or disorder.

BACKGROUND OF THE INVENTION

[0001] Cardiovascular disease is a major health risk throughout theindustrialized world. Coronary artery disease (CAD), or atherosclerosis,involves the progressional narrowing of the arteries due to a build-upof atherosclerotic plaque. Myocardial infarction (MI), e.g., heartattack, results when the heart is damaged due to reduced blood flow tothe heart caused by the build-up of plaque in the coronary arteries.

[0002] Coronary artery disease, the most prevalent of cardiovasculardiseases, is the principal cause of heart attack, stroke, and gangreneof the extremities, and thereby the principle cause of death in theUnited States. Coronary artery disease, or atherosclerosis, is a complexdisease involving many cell types and molecular factors (described in,for example, Ross, 1993, Nature 362: 801-809). The process, in normalcircumstances a protective response to insults to the endothelium andsmooth muscle cells (SMCs) of the wall of the artery, consists of theformation of fibrofatty and fibrous lesions or plaques, preceded andaccompanied by inflammation. The advanced lesions of atherosclerosis mayocclude the artery concerned, and result from an excessiveinflammatory-fibroproliferative response to numerous different forms ofinsult. Injury or dysfunction of the vascular endothelium is a commonfeature of may conditions that predispose a subject to accelerateddevelopment of atherosclerotic cardiovascular disease. For example,shear stresses are thought to be responsible for the frequent occurrenceof atherosclerotic plaques in regions of the circulatory system whereturbulent blood flow occurs, such as branch points and irregularstructures.

[0003] The first observable event in the formation of an atheroscleroticplaque occurs when blood-borne monocytes adhere to the vascularendothelial layer and transmigrate through to the sub-endothelial space.Adjacent endothelial cells at the same time produce oxidized low densitylipoprotein (LDL). These oxidized LDLs are then taken up in largeamounts by the monocytes through scavenger receptors expressed on theirsurfaces. In contrast to the regulated pathway by which native LDL(nLDL) is taken up by nLDL specific receptors, the scavenger pathway ofuptake is not regulated by the monocytes.

[0004] These lipid-filled monocytes are called foam cells, and are themajor constituent of the fatty streak. Interactions between foam cellsand the endothelial and SMCs which surround them lead to a state ofchronic local inflammation which can eventually lead to smooth musclecell proliferation and migration, and the formation of a fibrous plaque.

[0005] Such plaques occlude the blood vessel concerned and, thus,restrict the flow of blood, resulting in ischemia. Ischemia is acondition characterized by a lack of oxygen supply in tissues of organsdue to inadequate perfusion. Such inadequate perfusion can have a numberof natural causes, including atherosclerotic or restenotic lesions,anemia, or stroke. Many medical interventions, such as the interruptionof the flow of blood during bypass surgery, for example, also lead toischemia. In addition to sometimes being caused by diseasedcardiovascular tissue, ischemia may sometimes affect cardiovasculartissue, such as in ischemic heart disease. Ischemia may occur in anyorgan, however, that is suffering a lack of oxygen supply.

[0006] One of the most important risk factors for coronary arterydisease is a familial history. Although family history subsumes bothgenetic and shared environmental factors, studies suggest that CAD has avery strong genetic component (Marenberg, et al. (1994) NEJM 330:1041).Despite the importance of family history as a risk factor for CAD, it'sincomplete genetic basis has not been elucidated. Therefore, theidentification of genes which are involved in the development of CAD andMI would be beneficial.

[0007] It would thus be beneficial to identify polymorphic regionswithin genes which are associated with a vascular disease or disorder,such as coronary artery disease or myocardial infarction. It wouldfurther be desirable to provide prognostic, diagnostic, pharmacogenomic,and therapeutic methods utilizing the identified polymorphic regions.

SUMMARY OF THE INVENTION

[0008] The present invention is based, at least in part, on theidentification of polymorphic regions within thrombospondin genes, e.g.,thrombospondin 1 and thrombospondin 4 (referred to herein as THBS1 andTHBS4), which are associated with vascular disease, e.g., coronaryartery disease (CAD) and myocardial infarction (MI). Single nucleotidepolymorphisms (SNPs) within these polymorphic regions have been found tobe in linkage disequilibrium (LD) with other SNPs of the same geneswhich have been previously associated with specific diseases ordisorders, including vascular diseases or disorders.

[0009] The variant allele of a THBS1 SNP, identified as G334u4, waspreviously shown to be associated with increased risk for vasculardisease, e.g., CAD and MI. The present invention is based, at least inpart, on the discovery that three SNPs in the THBS1 gene (identifiedherein as G334u3, G334a18, G334u5 see Table 1), are in strong linkagedisequilibrium (LD) with G334u4. Two SNPs, identified herein as G334a16and G334k2, are in negative LD with G334u3. Therefore, G334u3, G334a18,G334u5, G334a16, and G334k2, and SNPs in LD therewith, act as markersfor G334u4 and can thus be used to predict risk of vascular disease,e.g., CAD and MI.

[0010] The variant allele of a THBS4 SNP, identified as G355u2, was alsopreviously shown to be associated with increased risk for vasculardisease, e.g., CAD and MI. The present invention is also based, at leastin part, on the discovery that two SNPs in the THBS4 gene (identifiedherein as G355a11 and TSC0656290 see Table 1), are in strong linkagedisequilibrium (LD) with the G355u2 SNP. Therefore, G355a11 andTSC0656290 and SNPs in LD therewith, act as markers for G355u2 and canthus be used to predict risk of cardiovascular disease, e.g., CAD andMI.

[0011] Thus, the invention relates to polymorphic regions and inparticular, SNPs identified as described herein, both singly and incombination, as well as to the use of these SNPs, and others in thesegenes, particularly those in linkage disequilibrium with these SNPs,both singly and in combination, for diagnosis, prediction of clinicalcourse of therapy and treatment response for vascular disease. The SNPsidentified herein may further be used in the development of newtreatments for vascular disease based upon comparison of the variant andnormal versions of the gene or gene product (e.g., the referencesequence), and development of cell-culture based and animal models forresearch and treatment of vascular disease. The invention furtherrelates to novel compounds and pharmaceutical compositions for use inthe diagnosis and treatment of such disorders. In preferred embodiments,the vascular disease is CAD or MI.

[0012] In one embodiment, the polymorphic regions of the invention areassociated with responsiveness to vascular disease or disordertherapies, e.g., clinical courses of therapy, including, but not limitedto lifestyle changes, medications, medical devices, such as adefibrillator, a stent, a device used in coronary revascularization, apacemaker, and any combination thereof, surgical or non-surgicalintervention or procedures such as percutaneous transluminal coronaryangioplasty, laser angioplasty, implantation of a stent, coronary bypassgrafting, implantation of a defibrillator, implantation of a pacemaker,and any combination thereof. The medical devices described in themethods of the invention can also be used in combination with amodulator of THBS1 and/or THBS4 gene expression or THBS1 and/or THBS4polypeptide activity.

[0013] Furthermore, the polymorphic regions of the invention are alsouseful in the determination of use of further diagnostic protocols,including, but not limited to, diagnostic vascular imaging, geneticanalysis, familial health history analysis, lifestyle analysis, exercisestress tests, or any combination thereof.

[0014] The polymorphisms of the invention may thus be used, both singly,or in combination, in prognostic, diagnostic, and therapeutic methods.For example, the polymorphisms of the invention can be used to determinewhether a subject has, or is, or is not at risk of developing a diseaseor disorder associated with a specific allelic variant of a THBS1 orTHBS4 polymorphic region, e.g., a disease or disorder associated withaberrant THBS1 or THBS4 activity, e.g., a vascular disease or disorder.

[0015] The invention thus relates to isolated nucleic acid molecules andmethods of using these molecules. The nucleic acid molecules of theinvention include specific allelic variants which differ from the THBS1and THBS4 reference sequences set forth in SEQ ID NO:1 (GI 12583762) orSEQ ID NO:3 (GI 14916146), respectively, or a portion thereof. Thepreferred nucleic acid molecules of the invention comprise THBS1 orTHBS4 polymorphic regions or portions thereof having the polymorphismsshown in Table 1, polymorphisms in linkage disequilibrium with thepolymorphisms shown in Table 1, and combinations thereof. Nucleic acidsof the invention can function as probes or primers, e.g., in methods fordetermining the allelic identity of a THBS1 or THBS4 polymorphic regionin a nucleic acid of interest.

[0016] The nucleic acids of the invention can also be used, singly or incombination, to determine whether a subject is or is not at risk ofdeveloping a disease associated with a specific allelic variant of aTHBS1 or THBS4 polymorphic region, e.g., a disease or disorderassociated with aberrant THBS1 or THBS4 activity, e.g., a vasculardisease or disorder such as CAD or MI. The nucleic acids of theinvention can further be used to prepare THBS1 or THBS4 polypeptidesencoded by specific alleles, such as mutant (variant) alleles. Suchpolypeptides can be used in therapy. Polypeptides encoded by specificTHBS1 or THBS4 alleles, such as variant THBS1 or THBS4 polypeptides, canalso be used as immunogens and selection agents for preparing, isolatingor identifying antibodies that specifically bind THBS1 or THBS4 proteinsencoded by these alleles. Accordingly, such antibodies can be used todetect variant THBS1 or THBS4 proteins.

[0017] There are six preferred polymorphisms identified in the THBS1gene. One polymorphism is a change from an adenine (A) to a guanine (G)in the THBS1 gene at residue 55322 of the reference sequence GI 12583762(polymorphism ID No. G334u4). This polymorphism results in a change froman asparagine (N) to a serine (S) in the amino acid sequence of THBS1(SEQ ID NO:2) at amino acid residue 700. A second polymorphism is achange from a thymidine (T) to a cytidine (C) in the THBS1 gene atresidue 53502 of the reference sequence GI 12583762 (polymorphism ID No.G334u3). This polymorphism does not result in a change in the amino acidsequence of the THBS1 protein (it is a “silent” variant). A thirdpolymorphism is a change from a cytidine (C) to a thymidine (T) in theTHBS1 gene at residue 60793 of the reference sequence GI 12583762(polymorphism ID No. G334a18). This polymorphism is not present in thecoding sequence of the THBS1 gene, and therefore does not result in achange in the amino acid sequence of the THBS1protein (it is a“non-coding” variant). A fourth polymorphism is a change from athymidine (T) to a cytidine (C) in the THBS1 gene at residue 58445 ofthe reference sequence GI 12583762 (polymorphism ID No. G334u5). Thispolymorphism is a silent variation. A fifth polymorphism is a changefrom an adenine (A) to a guanine (G) in the THBS1 gene at residue 52861of the reference sequence GI 12583762 (polymorphism ID No. G334a16).This polymorphism is a silent variant. A sixth polymorphism is a changefrom a thymidine (T) to a cytidine (C) in the THBS1 gene at residue49556 of the reference sequence GI 12583762 (polymorphism ID No.G334k2). This polymorphism is a non-coding variant. The six THBS1polymorphisms described above are in linkage disequilibrium with eachother.

[0018] There are three preferred polymorphisms identified in the THBS4gene. The first polymorphism in the THBS4 gene is a change from aguanine (G) to a cytidine (C) in the THBS4 gene at residue 105290 of thereference sequence GI 14916146 (polymorphism ID No. G355u2). Thispolymorphism results in a change from an alanine (A) to a proline (P) inthe amino acid sequence of THBS4 (SEQ ID NO:4) at amino acid residue387. The second THBS4 polymorphism is a change from a guanine (G) to anadenine (A) in the THBS4 gene at residue 118019 of the referencesequence GI 14916146 (polymorphism ID No. G355a11). This polymorphismresults in a change from a glycine (G) to a serine (S) in the amino acidsequence of THBS4 (SEQ ID NO:4) at amino acid residue 718. The thirdTHBS4 polymorphism is a change from a guanine (G) to an adenine (A) inthe THBS4 gene at residue 89835 of the reference sequence GI 14916146(polymorphism ID No. TSC0656290). This polymorphism is not present inthe coding sequence of the THBS1 gene, and therefore does not result ina change in the amino acid sequence of the THBS1 protein (it is a“non-coding” variant). These three THBS4 polymorphisms are in linkagedisequilibrium with each other.

[0019] The nucleic acid molecules of the invention can be double- orsingle-stranded. Accordingly, in one embodiment of the invention, acomplement of the nucleotide sequence is provided wherein thepolymorphism has been identified. For example, where there has been asingle nucleotide change from an adenine to a guanine in a singlestrand, the complement of that strand will contain a change from athymidine to a cytidine at the corresponding nucleotide residue. Theinvention further provides allele-specific oligonucleotides thathybridize to a gene comprising a polymorphism of the present inventionor to its complement.

[0020] The polymorphisms of the present invention, singly, incombination with each other, or in combination with previouslyidentified polymorphisms, are shown herein to be associated withspecific disorders, e.g., vascular diseases or disorders. Examples ofvascular diseases or disorders include, without limitation,atherosclerosis, coronary artery disease (CAD), myocardial infarction(MI), ischemia, stroke, peripheral vascular diseases, venousthromboembolism and pulmonary embolism.

[0021] The invention further provides vectors comprising the nucleicacid molecules of the present invention; host cells transfected withsaid vectors whether prokaryotic or eukaryotic; and transgenic non-humananimals which contain a heterologous form of a functional ornon-functional THBS1 and/or THBS4 allele described herein. Such atransgenic animal can serve as an animal model for studying the effectof specific THBS1 and/or THBS4 allelic variations, including mutations,as well as for use in drug screening and/or recombinant proteinproduction.

[0022] The invention further provides methods for determining at least aportion of a THBS1 and/or THBS4 gene. In a preferred embodiment, themethod comprises contacting a sample nucleic acid comprising a THBS1and/or THBS4 gene sequence with a probe or primer having a sequencewhich is complementary to a THBS1 and/or THBS4 gene sequence, carryingout a reaction that would amplify and/or detect differences in a regionof interest within the THBS1 and/or THBS4 gene sequence, and comparingthe result of each reaction with that of a reaction with a control(known) THBS1 and/or THBS4 gene (e.g., a THBS1and/or THBS4 gene from ahuman not afflicted with a vascular disease or disorder e.g., CAD, MI,or another disease associated with an aberrant THBS1 and/or THBS4activity) so as to determine the molecular structure of the THBS1 orTHBS4 gene sequence in the sample nucleic acid. The method of theinvention can be used for example in determining the molecular structureof at least a portion of an exon, an intron, a 5′ upstream regulatoryelement, or the 3′ untranslated region. In a preferred embodiment, themethod comprises determining the identity of at least one nucleotide. Inanother preferred embodiment, the method comprises determining theidentity of more than one nucleotide. In yet another preferredembodiment, the nucleotide is residue 55322, residue 53502, residue60793, residue 58445, residue 52861, and/or residue 49556 of thereference sequence GI 12583762 (the THBS1 gene), and/or residue 274474,residue 118019, and/or residue 89835 of the reference sequence GI14916146 (the THBS4 gene).

[0023] In another preferred embodiment, the method comprises determiningthe nucleotide content of at least a portion of a THBS1 or THBS4 gene,such as by sequence analysis. In yet another embodiment, determining themolecular structure of at least a portion of a THBS1 or THBS4 gene iscarried out by single-stranded conformation polymorphism (SSCP). In yetanother embodiment, the method is an oligonucleotide ligation assay(OLA). Other methods within the scope of the invention for determiningthe molecular structure of at least a portion of a THBS1 or THBS4 geneinclude hybridization of allele-specific oligonucleotides, sequencespecific amplification, primer specific extension, and denaturing highperformance liquid chromatography (DHPLC). In at least some of themethods of the invention, the probe or primer is allele specific.Preferred probes or primers are single stranded nucleic acids, whichoptionally are labeled.

[0024] The methods of the invention can be used for determining theidentity of a nucleotide or amino acid residue within a polymorphicregion of a human THBS1 and/or THBS4 gene present in a subject. Forexample, the methods of the invention can be useful for determiningwhether a subject has, or is or is not at risk of developing, a diseaseor condition associated with a specific allelic variant of a polymorphicregion in the human THBS1 and/or THBS4 gene, e.g., a vascular disease ordisorder.

[0025] In one embodiment, the disease or condition is characterized byan aberrant THBS1 and/or THBS4 activity, such as aberrant THBS1 and/orTHBS4 protein level, which can result from aberrant expression of aTHBS1 and/or THBS4 gene. The disease or condition can be CAD, MI, oranother vascular disease. Accordingly, the invention provides methodsfor predicting vascular diseases associated with aberrant THBS1 and/orTHBS4 activity.

[0026] The invention also provides a method of identifying subjectswhich are at increased risk of developing CAD and/or MI, wherein themethod comprises the steps of i) identifying in DNA from a subject atleast one sequence polymorphism, as compared with the reference THBS1and/or THBS4 gene sequence which comprises SEQ ID NOs:1 and 3,respectively, in a THBS1 and/or THBS4 gene sequence; and ii) identifyingthe subject based on the identified polymorphism.

[0027] In another embodiment, the invention also provides a method foridentifying a subject as a candidate for a particular clinical course oftherapy for a vascular disease or disorder, e.g., CAD or MI, forexample, treatment with medications, lifestyle changes, use of medicaldevices such as a defibrillator, a stent, a device used in coronaryrevascularization, a pacemaker, and any combination thereof and/orsurgical devices, such as, but not limited to, angioplasty devices, usedin, for example, surgical procedures such as percutaneous transluminalcoronary balloon angioplasty (PTCA) or laser angioplasty, implantationof a stent, or surgical intervention, such as coronary bypass grafting(CABG), or any combination thereof, wherein the method comprises thesteps of obtaining a nucleic acid sample from the subject, determiningone or more of the nucleotides present at nucleotide positions 55322,53502, 60793, 58445, 52861, and/or 49556 of SEQ ID NO:1, and/or theidentity of the nucleotide present at nucleotide position 105290,118019, and/or 89835 of SEQ ID NO:3, or the complement thereof; andidentifying the subject based on the identified nucleotides as a subjectwho is a candidate for a particular clinical course of therapy for avascular disease or disorder.

[0028] In yet another embodiment, the invention provides a method ofidentifying a subject as a candidate for further diagnostic evaluationfor a vascular disease or disorder or for the risk of a vascular diseaseor disorder, such as, for example, cardiovascular imaging, such asangiography, cardiac ultrasound, coronary angiogram, magnetic resonanceimagery, nuclear imaging, CT, myocardial perfusion imagery, orelectrocardiogram, genetic analysis, e.g., identification of additionalpolymorphisms, familial health history analysis, lifestyle analysis, orexercise stress tests, alone or in combination, wherein the methodcomprises the steps of obtaining a nucleic acid sample from the subject,determining one or more of the nucleotides present at nucleotidepositions 55322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ IDNO:1, and/or the nucleotide present at nucleotide position 105290,118019, and/or 89835 of SEQ ID NO:3, or the complement thereof; andidentifying the subject based on the identified nucleotides as a subjectwho is or is not a candidate for further diagnostic evaluation, or whowould or would not benefit from further diagnostic evaluation for avascular disease or disorder.

[0029] In a further embodiment, the invention provides a method fortreating a subject having a disease or condition associated with aspecific allelic variant of a polymorphic region of a THBS1 and/or THBS4gene. In one embodiment, the method comprises the steps of (a)determining the identity of the allelic variant; and (b) administeringto the subject a clinical course of therapy that compensates for theeffect of the specific allelic variant e.g., treatment with medications,lifestyle changes, surgical devices, such as, but not limited to,angioplasty devices, used in, for example, percutaneous transluminalcoronary balloon angioplasty (PTCA) or laser angioplasty, implantationof a stent, or surgical procedures, such as percutaneous transluminalcoronary angioplasty, laser angioplasty, implantation of a stent,coronary bypass grafting, implantation of a defibrillator, implantationof a pacemaker, and any combination thereof. In one embodiment, theclinical course of therapy is administration of an agent or modulatorwhich modulates, e.g., agonizes or antagonizes, THBS1 or THBS4 nucleicacid expression or THBS1 or THBS4 protein levels. In a preferredembodiment, the modulator is selected from the group consisting of anucleic acid, a ribozyme, an antisense THBS1 or THBS4 nucleic acidmolecule, a THBS1 or THBS4 protein or polypeptide, an antibody, apeptidomimetic, or a small molecule.

[0030] In a preferred embodiment, the specific allelic variant is amutation. The mutation can be located, e.g., in a 5′ upstream regulatoryelement, a 3′ regulatory element, an intron, or an exon of the gene.Thus, for example, in a subject having one or more of the variantalleles at nucleotide positions 55322, 53502, 60793, 58445, 52861,and/or 49556 of SEQ ID NO:1, and/or the variant alleles of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof, vascular disorders such asCAD or MI, can be treated, prevented, or ameliorated by administering tothe subject a particular clinical course of treatment sufficient totreat, prevent, or ameliorate the vascular disease or disorder.

[0031] Additionally, the invention provides a method of identifying asubject who is susceptible to a vascular disorder, which methodcomprises the steps of i) providing a nucleic acid sample from asubject; and ii) detecting in the nucleic acid sample a THBS1 or THBS4gene polymorphism, or one or more in combination, that correlate withthe vascular disorder with a P value less than or equal to 0.05, theexistence of the polymorphism being indicative of susceptibility to thevascular disorder.

[0032] The invention also provides a method of treating vasculardisorders which method comprises the step of i) identifying in geneticmaterial of a subject a THBS1 and/or THBS4 gene polymorphism thatcorrelates with increased responsiveness to a clinical course oftreatment as compared with responsiveness of a subject lacking thepolymorphism; and ii) administering the clinical course of therapy tothe subject.

[0033] The invention further provides forensic methods based ondetection of polymorphisms within the THBS1 or THBS4 genes.

[0034] The invention also provides probes and primers comprisingoligonucleotides, which correspond to a region of nucleotide sequencewhich hybridizes to at least 6 consecutive nucleotides of the sequenceset forth as SEQ ID NOs:5, 6, 7, 8, 9, 10, 11, 12, or 13, or to thecomplement of the sequences set forth as SEQ ID NOs:5, 6, 7, 8, 9, 10,11, 12, or 13, or naturally occurring mutants or variants thereof. Inpreferred embodiments, the probe/primer further includes a labelattached thereto, which is capable of being detected.

[0035] In another embodiment, the invention provides a kit foramplifying and/or for determining the molecular structure of at least aportion of a THBS1 or THBS4 gene, comprising a probe or primer capableof hybridizing to a THBS1 or THBS4 gene and instructions for use. In apreferred embodiment, determining the molecular structure of a region ofa THBS1 or THBS4 gene comprises determining the identity of the allelicvariant of the polymorphic region. Determining the molecular structureof at least a portion of a THBS1 or THBS4 gene can comprise determiningthe identity of at least one nucleotide or determining the nucleotidecomposition, e.g., the nucleotide sequence a THBS1 or THBS4 gene.

[0036] A kit of the invention can be used, e.g., for determining whethera subject is or is not at risk of developing a disease associated with aspecific allelic variant of a polymorphic region of a THBS1 or THBS4gene, e.g., CAD or MI. In a preferred embodiment, the invention providesa kit for determining whether a subject is or is not at risk ofdeveloping a vascular disease such as, for example, atherosclerosis,CAD, MI, ischemia, stroke, peripheral vascular diseases, venousthromboembolism and pulmonary embolism. The kit of the invention canalso be used in selecting the appropriate clinical course of clinicaltreatment to a subject to treat a disease or condition, such as adisease or condition set forth above. Thus, determining the allelicvariants of THBS1 or THBS4 polymorphic regions of a subject can beuseful in predicting how a subject will respond to a specific drug,e.g., a drug for treating a disease or disorder associated with aberrantTHBS1 or THBS4, e.g., a vascular disease or disorder.

[0037] Other features and advantages of the invention will be apparentfrom the following detailed description and claims.

BRIEF DESCRIPTION OF THE FIGURES

[0038]FIG. 1 depicts the nucleotide sequence corresponding to referencesequence GI 12583762 (SEQ ID NO:1) for the THBS1 gene.

[0039]FIG. 2 depicts the reference amino acid sequence for the THBS1protein (SEQ ID NO:2).

[0040]FIG. 3 depicts the nucleotide sequence corresponding to referencesequence GI 14916146 (SEQ ID NO:3) for the THBS4 gene.

[0041]FIG. 4 depicts the reference amino acid sequence for the THBS4protein (SEQ ID NO:4).

DETAILED DESCRIPTION OF THE INVENTION

[0042] The present invention is based, at least in part, on thediscovery that three SNPs in the THBS1 gene (identified herein asG334u3, G334a18, and G334u5, see Table 1), are in strong linkagedisequilibrium (LD) with G334u4, and are associated with increased riskof vascular disease such as CAD and MI. Two SNPs in the THBS1 gene,identified herein as G334a16, and G334k2, are in negative LD with G334u4and have a protective effect for vascular disease, e.g., a subject withthe variant alleles are less likely to have vascular disease, e.g., CADor MI. The variant allele of G334u4 was previously shown to beassociated with increased risk for vascular disease, e.g., CAD and MI.Therefore, G334u3, G334a18, G334u5, G334a16, and G334k2 act as markersfor G334u4 and can be used to predict risk of cardiovascular disease,e.g., CAD and MI.

[0043] The present invention is also based, at least in part, on thediscovery that two SNPs in the THBS4 gene (identified herein as G355a11and TSC0656290 see Table 1), are in linkage disequilibrium (LD) withG355u2 and are associated with increased risk for vascular disease suchas CAD and MI. The variant allele of G355u2 was previously shown to beassociated with increased risk for vascular disease, e.g., CAD and MI.Therefore, G355a11 and TSC0656290 act as markers for G355u2 and can beused to predict risk of cardiovascular disease, e.g., CAD and MI.

[0044] The term “linkage” describes the tendency of genes, alleles, locior genetic markers to be inherited together as a result of theirlocation on the same chromosome. It can be measured by percentrecombination between the two genes, alleles, loci, or genetic markers.The term “linkage disequilibrium,” also referred to herein as “LD,”refers to a greater than random association between specific alleles attwo marker loci within a particular population. In general, linkagedisequilibrium decreases with an increase in physical distance. Iflinkage disequilibrium exists between two markers, or SNPs, then thegenotypic information at one marker, or SNP, can be used to makeprobabilistic predictions about the genotype of the second marker.

[0045] The polymorphisms of the present invention are single nucleotidepolymorphisms (SNPs) at a specific nucleotide residue within the THBS1and THBS4 genes. The THBS1 and THBS4 genes have at least two alleles,referred to herein as the reference allele and the variant allele. Thereference alleles (i.e., the consensus sequence, or wild type allele)have been designated based on their frequency in a general U.S.Caucasian population sample. The reference allele is the more common ofthe two alleles; the variant is the more rare of the two alleles.Nucleotide sequences in GenBank may correspond to either allele andcorrespond to the nucleotide sequence of the nucleotide sequence whichhas been deposited in GenBank™ and given a specific Accession Number(e.g., GI 12583762, the reference sequence for the THBS1 gene or GI14916146, the reference sequence for the THBS4 gene corresponding to SEQID NO:1 and SEQ ID NO:3, respectively). The reference sequence for theamino acid sequences of THBS1 and THBS4 proteins are set forth as SEQ IDNO:2 and SEQ ID NO:4, respectively. The variant allele differs from thereference allele by at least one nucleotide at the site(s) identified inTable 1, and those in linkage disequilibrium therewith. The presentinvention thus relates to nucleotides comprising variant alleles of theTHBS1 and THBS4 reference sequences and/or complements of the variantalleles to be used singly or in combination to predict the risk ofvascular disease.

[0046] The SNPs of the THBS1 gene as described herein, which are inlinkage disequilibrium with each other, can be used singly or incombination to predict the risk of vascular disease in a subject.Likewise, the SNPs of the THBS4 gene, as described herein, which are inlinkage disequilibrium with each other, can be used singly or incombination to predict the risk of vascular disease in a subject.

[0047] The invention further relates to nucleotides comprising portionsof the variant alleles and/or portions of complements of the variantalleles which comprise the site of the polymorphism and are at least 5nucleotides or basepairs in length. Portions can be, for example, 5-10,5-15, 10-20, 2-25, 10-30, 10-50 or 10-100 bases or basepairs long. Forexample, a portion of a variant allele which is 17 nucleotides orbasepairs in length includes the polymorphism (i.e., the nucleotide(s)which differ from the reference allele at that site) and twentyadditional nucleotides or basepairs which flank the site in the variantallele. These additional nucleotides and basepairs can be on one or bothsides of the polymorphism. Polymorphisms which are the subject of thisinvention are defined in Table 1 with respect to the reference sequencesidentified in Table 1, and those polymorphisms in linkage disequilibriumwith the polymorphisms of Table 1.

[0048] It is understood that the invention is not limited by theseexemplified reference sequences, as variants of these sequences whichdiffer at locations other than the SNP sites identified herein can alsobe utilized. The skilled artisan can readily determine the SNP sites inthese other reference sequences which correspond to the SNP sitesidentified herein by aligning the sequence of interest with thereference sequences specifically disclosed herein, and programs forperforming such alignments are commercially available. For example, theALIGN program in the GCG software package can be used, utilizing aPAM120 weight residue table, a gap length penalty of 12 and a gappenalty of 4, for example.

[0049] The polymorphic region of the present invention is associatedwith specific diseases or disorders and has been identified in the humanF7 gene by analyzing the DNA of cell lines derived from an ethnicallydiverse population by methods described in Cargill, et al. (1999) NatureGenetics 22:231-238.

[0050] Cases which were used to identify associations between vasculardisease and SNPs were comprised of 352 U.S. Caucasian subject withpremature coronary artery disease were identified in 15 participatingmedical centers, fulfilling the criteria of either myocardialinfarction, surgical or percutaneous revascularization, or a significantcoronary artery lesion diagnosed before age 45 in men or age 50 in womenand having a living sibling who met the same criteria. These cases werecompared with a random sample of 418 Caucasian controls drawn from thegeneral U.S. population in Atlanta, Ga.

[0051] The allelic variants of the present invention were identified byperforming denaturing high performance liquid chromatography (DHPLC)analysis, variant detector arrays (Affymetrix™), the polymerase chainreaction (PCR), and/or single stranded conformation polymorphism (SSCP)analysis of genomic DNA from independent individuals as described in theExamples, using PCR primers complementary to intronic sequencessurrounding each of the exons, 3′ UTR, and 5′ upstream regulatoryelement sequences of the human THBS1 and THBS4 genes.

[0052] The presence of at least one polymorphism in the human THBS1 andTHBS4 genes in the population studied were identified. Each of thevariants are characterized as single nucleotide polymorphisms (SNPs).The preferred polymorphisms of the invention are listed in Table 1.

[0053] Table 1 contains a “polymorphism ID No.” in column 2, which isused herein to identify each individual variant, e.g., G334u4, G334u3,G334a18, G334u5, G334a16, G334k2, G355u2, G355a11, and TSC0656290. InTable 1, the nucleotide sequence flanking each polymorphism is providedin column 9, wherein the polymorphic residue(s), having the referencenucleotide, is indicated in lower-case letters. There are either 8 or 15nucleotides flanking the polymorphic nucleotide residue (i.e., 8nucleotides 5′ of the polymorphism and 8 nucleotides 3′ of thepolymorphism). Column 10 indicates the SEQ ID NO. that is used toidentify each polymorphism. SEQ ID NOs: 5, 6, 7, 8, 9, 10, 11, 12, or 13comprise sequences shown in column 9 with the variant nucleotide residueat the residue(s) indicated by a lower-case letter.

[0054] Each polymorphism is identified based on a change in thenucleotide sequence from a consensus sequence, or the “referencesequence.” As used herein, the reference sequence of THBS1 is thenucleotide sequence of SEQ ID NO:1 which corresponds to GI 12583762 (seeFIG. 1), the reference sequence of THBS4 is the nucleotide sequence ofSEQ ID NO:3 which corresponds to GI 14916146 (see FIG. 3).

[0055] To identify the location of each polymorphism in Table 1, aspecific nucleotide residue in a reference sequence is listed for eachpolymorphism, where nucleotide residue number 1 is the first (i.e., 5′)nucleotide in each reference sequence. Column 8 lists the referencesequence and polymorphic nucleotide residue for each polymorphism.Column 4 describes the type of variant for each SNP, e.g., missense(mis), silent, or non-coding.

[0056] The nucleic acid molecules of the invention can be double- orsingle-stranded. Accordingly, the invention further provides for thecomplementary nucleic acid strands comprising the polymorphisms listedin Table 1.

[0057] The invention further provides allele-specific oligonucleotidesthat hybridize to a gene comprising a single nucleotide polymorphism orto the complement of the gene. Such oligonucleotides will hybridize toone polymorphic form of the nucleic acid molecules described herein butnot to the other polymorphic form(s) of the sequence. Thus sucholigonucleotides can be used to determine the presence or absence ofparticular alleles of the polymorphic sequences described herein. Theseoligonucleotides can be probes or primers.

[0058] Not only does the present invention provide polymorphisms inlinkage disequilibrium with the polymorphisms of Table 1, it alsoprovides methods for revealing the existence of yet other polymorphicregions in the human THBS1 or THBS4 gene. For example, the polymorphismstudies described herein can also be applied to populations in whichother vascular diseases or disorders are prevalent.

[0059] Other aspects of the invention are described below or will beapparent to one of skill in the art in light of the present disclosure.

[0060] Definitions

[0061] For convenience, the meaning of certain terms and phrasesemployed in the specification, examples, and appended claims areprovided below.

[0062] The term “allele”, which is used interchangeably herein with“allelic variant” refers to alternative forms of a gene or portionsthereof. Alleles occupy the same locus or position on homologouschromosomes. When a subject has two identical alleles of a gene, thesubject is said to be homozygous for the gene or allele. When a subjecthas two different alleles of a gene, the subject is said to beheterozygous for the gene or allele. Alleles of a specific gene,including the THBS1 or THBS4 genes, can differ from each other in asingle nucleotide, or several nucleotides, and can includesubstitutions, deletions, and insertions of nucleotides. An allele of agene can also be a form of a gene containing one or more mutations.

[0063] The term “allelic variant of a polymorphic region of a THBS1 orTHBS4 gene” refers to an alternative form of the THBS1 or THBS4 genehaving one of several possible nucleotide sequences found in that regionof the gene in the population.

[0064] “Biological activity” or “bioactivity” or “activity” or“biological function”, which are used interchangeably, for the purposesherein when applied to THBS1 or THBS4, means an effector or antigenicfunction that is directly or indirectly performed by a THBS1 or THBS4polypeptide (whether in its native or denatured conformation), or by afragment thereof. Biological activities include modulation of thedevelopment of atherosclerotic plaque leading to vascular disease andother biological activities, whether presently known or inherent. ATHBS1 or THBS4 bioactivity can be modulated by directly affecting aTHBS1 or THBS4 protein effected by, for example, changing the level ofeffector or substrate level. Alternatively, a THBS1 or THBS4 bioactivitycan be modulated by modulating the level of a THBS1 or THBS4 protein,such as by modulating expression of a THBS1 or THBS4 gene. Antigenicfunctions include possession of an epitope or antigenic site that iscapable of cross-reacting with antibodies that bind a native ordenatured THBS1 or THBS4 polypeptide or fragment thereof.

[0065] Biologically active THBS1 or THBS4 polypeptides includepolypeptides having both an effector and antigenic function, or only oneof such functions. THBS1 or THBS4 polypeptides include antagonistpolypeptides and native THBS1 or THBS4 polypeptides, provided that suchantagonists include an epitope of a native THBS1 or THBS4 polypeptide.An effector function of THBS1 or THBS4 polypeptide can be the ability tobind to a ligand of a THBS1 or THBS4 molecule.

[0066] As used herein the term “bioactive fragment of a THBS1 or THBS4protein” refers to a fragment of a full-length THBS1 or THBS4 protein,wherein the fragment specifically mimics or antagonizes the activity ofa wild-type THBS1 or THBS4 protein. The bioactive fragment preferably isa fragment capable of binding to a second molecule, such as a ligand.

[0067] The term “an aberrant activity” or “abnormal activity”, asapplied to an activity of a protein such as THBS1 or THBS4, refers to anactivity which differs from the activity of the normal or referenceprotein or which differs from the activity of the protein in a healthysubject, e.g., a subject not afflicted with a disease associated with aTHBS1 or THBS4 allelic variant. An activity of a protein can be aberrantbecause it is stronger than the activity of its wild-type counterpart.Alternatively, an activity of a protein can be aberrant because it isweaker or absent relative to the activity of its normal or referencecounterpart. An aberrant activity can also be a change in reactivity.For example an aberrant protein can interact with a different protein orligand relative to its normal or reference counterpart. A cell can alsohave aberrant THBS1 or THBS4 activity due to overexpression orunderexpression of the THBS1 or THBS4 gene. Aberrant THBS1 or THBS4activity can result from a mutation in the gene, which results, e.g., inlower or higher binding affinity of a ligand to the THBS1 or THBS4protein encoded by the mutated gene. Aberrant THBS1 or THBS4 activitycan also result from an abnormal THBS1 or THBS4 5′ upstream regulatoryelement activity.

[0068] “Cells,” “host cells” or “recombinant host cells” are terms usedinterchangeably herein. It is understood that such terms refer not onlyto the particular cell but to the progeny or derivatives of such a cell.Because certain modifications may occur in succeeding generations due toeither mutation or environmental influences, such progeny may not, infact, be identical to the parent cell, but are still included within thescope of the term as used herein.

[0069] As used herein, the term “course of clinical therapy” refers toany chosen method to treat, prevent, or ameliorate a vascular disease,e.g., CAD or MI, symptoms thereof, or related diseases or disorders.Courses of clinical therapy include, but are not limited to, lifestylechanges (e.g., changes in diet or environment), administration ofmedication, use of medical devices, such as, but not limited to, adefibrillator, a stent, a device used in coronary revascularization, apacemaker, or any combination thereof, and surgical procedures such aspercutaneous transluminal coronary balloon angioplasty (PTCA) or laserangioplasty, or other surgical intervention, such as, for example,coronary bypass grafting (CABG), or any combination thereof.

[0070] As used herein, the term “gene” or “recombinant gene” refers to anucleic acid molecule comprising an open reading frame and including atleast one exon and (optionally) an intron sequence. The term “intron”refers to a DNA sequence present in a given gene which is spliced outduring mRNA maturation.

[0071] As used herein, the term “genetic profile” refers to theinformation obtained from identification of the specific allelicvariants of a subject. For example, a THBS1 genetic profile refers tothe specific allelic variants of a subject within the THBS1 gene. Forexample, one can determine a subject's THBS1 genetic profile bydetermining the identity of one or more of the nucleotides present atnucleotide 5322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ ID NO:1(the THBS1 gene). One can determine a subject's THBS4 genetic profile bydetermining the identity of one or more of the nucleotides present atnucleotide position 105290, 118019, and/or 89835 of SEQ ID NO:3 (theTHBS4 gene). The genetic profile of a particular disease can beascertained through identification of the identity of allelic variantsin one or more genes which are associated with the particular disease.

[0072] “Homology” or “identity” or “similarity” refers to sequencesimilarity between two peptides or between two nucleic acid molecules.Homology can be determined by comparing a position in each sequencewhich may be aligned for purposes of comparison. When a position in thecompared sequence is occupied by the same base or amino acid, then themolecules are homologous at that position. A degree of homology betweensequences is a function of the number of matching or homologouspositions shared by the sequences. An “unrelated” or “non-homologous”sequence shares less than 40% identity, though preferably less than 25%identity, with one of the sequences of the present invention.

[0073] To determine the percent identity of two amino acid sequences orof two nucleic acids, the sequences are aligned for optimal comparisonpurposes (e.g., gaps can be introduced in the sequence of a first aminoacid or nucleic acid sequence for optimal alignment with a second aminoor nucleic acid sequence). The amino acid residues or nucleotides atcorresponding amino acid positions or nucleotide positions are thencompared. When a position in the first sequence is occupied by the sameamino acid residue or nucleotide as the corresponding position in thesecond sequence, then the molecules are identical at that position. Thepercent identity between the two sequences is a function of the numberof identical positions shared by the sequences (i.e., % identity=numberof identical positions/total number of positions (e.g., overlappingpositions)×100). In one embodiment the two sequences are the samelength.

[0074] The determination of percent identity between two sequences canbe accomplished using a mathematical algorithm. A preferred,non-limiting example of a mathematical algorithm utilized for thecomparison of two sequences is the algorithm of Karlin and Altschul(1990) Proc. Natl. Acad. Sci. USA 87:2264-2268, modified as in Karlinand Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-5877. Such analgorithm is incorporated into the NBLAST and XBLAST programs ofAltschul, et al. (1990) J. Mol. Biol. 215:403-410. BLAST nucleotidesearches can be performed with the NBLAST program, score=100,wordlength=12 to obtain nucleotide sequences homologous to a nucleicacid molecules of the invention. BLAST protein searches can be performedwith the XBLAST program, score=50, wordlength=3 to obtain amino acidsequences homologous to a protein molecules of the invention. To obtaingapped alignments for comparison purposes, Gapped BLAST can be utilizedas described in Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402.Alternatively, PSI-Blast can be used to perform an iterated search whichdetects distant relationships between molecules. When utilizing BLAST,Gapped BLAST, and PSI-Blast programs, the default parameters of therespective programs (e.g., XBLAST and NBLAST) can be used. Anotherpreferred, non-limiting example of a mathematical algorithm utilized forthe comparison of sequences is the algorithm of Myers and Miller, (1988)CABIOS 4:11-17. Such an algorithm is incorporated into the ALIGN program(version 2.0) which is part of the GCG sequence alignment softwarepackage. When utilizing the ALIGN program for comparing amino acidsequences, a PAM120 weight residue table, a gap length penalty of 12,and a gap penalty of 4 can be used. Yet another useful algorithm foridentifying regions of local sequence similarity and alignment is theFASTA algorithm as described in Pearson and Lipman (1988) Proc. Natl.Acad. Sci. USA 85:2444-2448. When using the FASTA algorithm forcomparing nucleotide or amino acid sequences, a PAM120 weight residuetable can, for example, be used with a k-tuple value of 2.

[0075] The term “a homolog of a nucleic acid” refers to a nucleic acidhaving a nucleotide sequence having a certain degree of homology withthe nucleotide sequence of the nucleic acid or complement thereof. Forexample, a homolog of a double stranded nucleic acid having SEQ ID NO:Nis intended to include nucleic acids having a nucleotide sequence whichhas a certain degree of homology with SEQ ID NO:N or with the complementthereof. Preferred homologs of nucleic acids are capable of hybridizingto the nucleic acid or complement thereof.

[0076] The term “hybridization probe” or “primer” as used herein isintended to include oligonucleotides which hybridize bind in abase-specific manner to a complementary strand of a target nucleic acid.Such probes include peptide nucleic acids, and described in Nielsen etal., (1991) Science 254:1497-1500. Probes and primers can be any lengthsuitable for specific hybridization to the target nucleic acid sequence.The most appropriate length of the probe and primer may vary dependingon the hybridization method in which it is being used; for example,particular lengths may be more appropriate for use in microfabricatedarrays, while other lengths may be more suitable for use in classicalhybridization methods. Such optimizations are known to the skilledartisan. Suitable probes and primers can range form about 5 nucleotidesto about 30 nucleotides in length. For example, probes and primers canbe 5, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 25, 26, 28 or 30 nucleotidesin length. The probe or primer of the invention comprises a sequencethat flanks and/or preferably overlaps, at least one polymorphic siteoccupied by any of the possible variant nucleotides. The nucleotidesequence of an overlapping probe or primer can correspond to the codingsequence of the allele or to the complement of the coding sequence ofthe allele.

[0077] The term “vascular disease or disorder” as used herein refers toany disease or disorder effecting the vascular system, including theheart and blood vessels. A vascular disease or disorder includes anydisease or disorder characterized by vascular dysfunction, including,for example, intravascular stenosis (narrowing) or occlusion (blockage),due to the development of atherosclerotic plaque and diseases anddisorders resulting therefrom. Examples of vascular diseases anddisorders include, without limitation, atherosclerosis, CAD, MI,ischemia, stroke, peripheral vascular diseases, venous thromboembolismand pulmonary embolism.

[0078] The term “interact” as used herein is meant to include detectableinteractions between molecules, such as can be detected using, forexample, a binding or hybridization assay. The term interact is alsomeant to include “binding” interactions between molecules. Interactionsmay be, for example, protein-protein, protein-nucleic acid,protein-small molecule or small molecule-nucleic acid in nature.

[0079] The term “intronic sequence” or “intronic nucleotide sequence”refers to the nucleotide sequence of an intron or portion thereof.

[0080] The term “isolated” as used herein with respect to nucleic acids,such as DNA or RNA, refers to molecules separated from other DNAs orRNAs, respectively, that are present in the natural source of themacromolecule. The term isolated as used herein also refers to a nucleicacid or peptide that is substantially free of cellular material, viralmaterial, or culture medium when produced by recombinant DNA techniques,or chemical precursors or other chemicals when chemically synthesized.Moreover, an “isolated nucleic acid” is meant to include nucleic acidfragments which are not naturally occurring as fragments and would notbe found in the natural state. The term “isolated” is also used hereinto refer to polypeptides which are isolated from other cellular proteinsand is meant to encompass both purified and recombinant polypeptides.

[0081] The term “linkage” describes the tendency of genes, alleles, locior genetic markers to be inherited together as a result of theirlocation on the same chromosome. It can be measured by percentrecombination between the two genes, alleles, loci, or genetic markers.The term “linkage disequilibrium,” also referred to herein as “LD,”refers to a greater than random association between specific alleles attwo marker loci within a particular population. In general, linkagedisequilibrium decreases with an increase in physical distance. Iflinkage disequilibrium exists between two markers, then the genotypicinformation at one marker can be used to make probabilistic predictionsabout the genotype of the second marker.

[0082] The term “locus” refers to a specific position in a chromosome.For example, a locus of a THBS1 or THBS4 gene refers to the chromosomalposition of the THBS1 or THBS4 gene.

[0083] The term “modulation” as used herein refers to both upregulation,(i e., activation or stimulation), for example by agonizing; anddownregulation (i.e. inhibition or suppression), for example byantagonizing of a bioactivity (e.g. expression of a gene).

[0084] The term “molecular structure” of a gene or a portion thereofrefers to the structure as defined by the nucleotide content (includingdeletions, substitutions, additions of one or more nucleotides), thenucleotide sequence, the state of methylation, and/or any othermodification of the gene or portion thereof.

[0085] The term “mutated gene” refers to an allelic form of a gene thatdiffers from the predominant form in a population. A mutated gene iscapable of altering the phenotype of a subject having the mutated generelative to a subject having the predominant form of the gene. If asubject must be homozygous for this mutation to have an alteredphenotype, the mutation is said to be recessive. If one copy of themutated gene is sufficient to alter the phenotype of the subject, themutation is said to be dominant. If a subject has one copy of themutated gene and has a phenotype that is intermediate between that of ahomozygous and that of a heterozygous subject (for that gene), themutation is said to be co-dominant.

[0086] As used herein, the term “nucleic acid” refers to polynucleotidessuch as deoxyribonucleic acid (DNA), and, where appropriate, ribonucleicacid (RNA). The term should also be understood to include, asequivalents, derivatives, variants and analogs of either RNA or DNA madefrom nucleotide analogs, and, as applicable to the embodiment beingdescribed, single (sense or antisense) and double-strandedpolynucleotides. Deoxyribonucleotides include deoxyadenosine,deoxycytidine, deoxyguanosine, and deoxythymidine. For purposes ofclarity, when referring herein to a nucleotide of a nucleic acid, whichcan be DNA or an RNA, the terms “adenine”, “cytidine”, “guanine”, and“thymidine” and/or “A”, “C”, “G”, and “T”, respectively, are used. It isunderstood that if the nucleic acid is RNA, a nucleotide having a uracilbase is uridine.

[0087] The term “nucleotide sequence complementary to the nucleotidesequence set forth in SEQ ID NO:N” refers to the nucleotide sequence ofthe complementary strand of a nucleic acid strand having SEQ ID NO:N.The term “complementary strand” is used herein interchangeably with theterm “complement”. The complement of a nucleic acid strand can be thecomplement of a coding strand or the complement of a non-coding strand.When referring to double stranded nucleic acids, the complement of anucleic acid having SEQ ID NO:N refers to the complementary strand ofthe strand having SEQ ID NO:N or to any nucleic acid having thenucleotide sequence of the complementary strand of SEQ ID NO:N. Whenreferring to a single stranded nucleic acid having the nucleotidesequence SEQ ID NO:N, the complement of this nucleic acid is a nucleicacid having a nucleotide sequence which is complementary to that of SEQID NO:N. The nucleotide sequences and complementary sequences thereofare always given in the 5′ to 3′ direction. The term “complement” and“reverse complement” are used interchangeably herein.

[0088] A “non-human animal” of the invention can include mammals such asrodents, non-human primates, sheep, goats, horses, dogs, cows, chickens,amphibians, reptiles, etc. Preferred non-human animals are selected fromthe rodent family including rat and mouse, most preferably mouse, thoughtransgenic amphibians, such as members of the Xenopus genus, andtransgenic chickens can also provide important tools for understandingand identifying agents which can affect, for example, embryogenesis andtissue formation. The term “chimeric animal” is used herein to refer toanimals in which an exogenous sequence is found, or in which anexogenous sequence is expressed in some but not all cells of the animal.The term “tissue-specific chimeric animal” indicates that an exogenoussequence is present and/or expressed or disrupted in some tissues, butnot others.

[0089] The term “oligonucleotide” is intended to include and single- ordouble stranded DNA or RNA. Oligonucleotides can be naturally occurringor synthetic, but are typically prepared by synthetic means. Preferredoligonucleotides of the invention include segments of THBS1 or THBS4gene sequence or their complements, which include and/or flank any oneof the polymorphic sites shown in Table 1. The segments can be between 5and 250 bases, and, in specific embodiments, are between 5-10, 5-20,10-20, 10-50, 20-50 or 10-100 bases. For example, the segments can be 21bases. The polymorphic site can occur within any position of the segmentor a region next to the segment. The segments can be from any of theallelic forms of THBS1 or THBS4 gene sequence shown in Table 1.

[0090] The term “operably-linked” is intended to mean that the 5′upstream regulatory element is associated with a nucleic acid in such amanner as to facilitate transcription of the nucleic acid from the 5′upstream regulatory element.

[0091] The term “polymorphism” refers to the coexistence of more thanone form of a gene or portion thereof. A portion of a gene of whichthere are at least two different forms, i.e., two different nucleotidesequences, is referred to as a “polymorphic region of a gene.” Apolymorphic locus can be a single nucleotide, the identity of whichdiffers in the other alleles. A polymorphic locus can also be more thanone nucleotide long. The allelic form occurring most frequently in aselected population is often referred to as the reference and/orwildtype form. Other allelic forms are typically designated oralternative or variant alleles. Diploid organisms may be homozygous orheterozygous for allelic forms. A diallelic or biallelic polymorphismhas two forms. A trialleleic polymorphism has three forms.

[0092] A “polymorphic gene” refers to a gene having at least onepolymorphic region.

[0093] The term “primer” as used herein, refers to a single-strandedoligonucleotide which acts as a point of initiation of template-directedDNA synthesis under appropriate conditions (e.g., in the presence offour different nucleoside triphosphates and as agent for polymerization,such as DNA or RNA polymerase or reverse transcriptase) in anappropriate buffer and at a suitable temperature. The length of a primermay vary but typically ranges from 15 to 30 nucleotides. A primer neednot match the exact sequence of a template, but must be sufficientlycomplementary to hybridize with the template.

[0094] The term “primer pair” refers to a set of primers including anupstream primer that hybridizes with the 3′ end of the complement of theDNA sequence to be amplified and a downstream primer that hybridizeswith the 3′ end of the sequence to be amplified.

[0095] The terms “protein”, “polypeptide” and “peptide” are usedinterchangeably herein when referring to a gene product.

[0096] The term “recombinant protein” refers to a polypeptide which isproduced by recombinant DNA techniques, wherein generally, DNA encodingthe polypeptide is inserted into a suitable expression vector which isin turn used to transform a host cell to produce the heterologousprotein.

[0097] A “regulatory element”, also termed herein “regulatory sequence”is intended to include elements which are capable of modulatingtranscription from a 5′ upstream regulatory sequence, including, but notlimited to a basic promoter, and include elements such as enhancers andsilencers. The term “enhancer”, also referred to herein as “enhancerelement”, is intended to include regulatory elements capable ofincreasing, stimulating, or enhancing transcription from a 5′ upstreamregulatory element, including a basic promoter. The term “silencer”,also referred to herein as “silencer element” is intended to includeregulatory elements capable of decreasing, inhibiting, or repressingtranscription from a 5′ upstream regulatory element, including a basicpromoter. Regulatory elements are typically present in 5′ flankingregions of genes. Regulatory elements also may be present in otherregions of a gene, such as introns. Thus, it is possible that THBS1 orTHBS4 genes have regulatory elements located in introns, exons, codingregions, and 3′ flanking sequences. Such regulatory elements are alsointended to be encompassed by the present invention and can beidentified by any of the assays that can be used to identify regulatoryelements in 5′ flanking regions of genes.

[0098] The term “regulatory element” further encompasses “tissuespecific” regulatory elements, i.e., regulatory elements which effectexpression of an operably linked DNA sequence preferentially in specificcells (e.g., cells of a specific tissue). Gene expression occurspreferentially in a specific cell if expression in this cell type issignificantly higher than expression in other cell types. The term“regulatory element” also encompasses non-tissue specific regulatoryelements, i.e., regulatory elements which are active in most cell types.Furthermore, a regulatory element can be a constitutive regulatoryelement, i.e., a regulatory element which constitutively regulatestranscription, as opposed to a regulatory element which is inducible,i.e., a regulatory element which is active primarily in response to astimulus. A stimulus can be, e.g., a molecule, such as a protein,hormone, cytokine, heavy metal, phorbol ester, cyclic AMP (cAMP), orretinoic acid.

[0099] Regulatory elements are typically bound by proteins, e.g.,transcription factors. The term “transcription factor” is intended toinclude proteins or modified forms thereof, which interactpreferentially with specific nucleic acid sequences, i.e., regulatoryelements, and which in appropriate conditions stimulate or represstranscription. Some transcription factors are active when they are inthe form of a monomer. Alternatively, other transcription factors areactive in the form of a dimer consisting of two identical proteins ordifferent proteins (heterodimer). Modified forms of transcriptionfactors are intended to refer to transcription factors having apostranslational modification, such as the attachment of a phosphategroup. The activity of a transcription factor is frequently modulated bya postranslational modification. For example, certain transcriptionfactors are active only if they are phosphorylated on specific residues.Alternatively, transcription factors can be active in the absence ofphosphorylated residues and become inactivated by phosphorylation. Alist of known transcription factors and their DNA binding site can befound, e.g., in public databases, e.g., TFMATRIX Transcription FactorBinding Site Profile database.

[0100] The term “single nucleotide polymorphism” (SNP) refers to apolymorphic site occupied by a single nucleotide, which is the site ofvariation between allelic sequences. The site is usually preceded by andfollowed by highly conserved sequences of the allele (e.g., sequencesthat vary in less than {fraction (1/100)} or {fraction (1/1000)} membersof a population). A SNP usually arises due to substitution of onenucleotide for another at the polymorphic site. SNPs can also arise froma deletion of a nucleotide or an insertion of a nucleotide relative to areference allele. Typically the polymorphic site is occupied by a baseother than the reference base. For example, where the reference allelecontains the base “T” (thymidine) at the polymorphic site, the alteredallele can contain a “C” (cytidine), “G” (guanine), or “A” (adenine) atthe polymorphic site.

[0101] SNP's may occur in protein-coding nucleic acid sequences, inwhich case they may give rise to a defective or otherwise variantprotein, or genetic disease. Such a SNP may alter the coding sequence ofthe gene and therefore specify another amino acid (a “missense” SNP) ora SNP may introduce a stop codon (a “nonsense” SNP). When a SNP does notalter the amino acid sequence of a protein, the SNP is called “silent.”SNP's may also occur in noncoding regions of the nucleotide sequence.This may result in defective protein expression, e.g., as a result ofalternative spicing, or it may have no effect.

[0102] As used herein, the term “specifically hybridizes” or“specifically detects” refers to the ability of a nucleic acid moleculeof the invention to hybridize to at least approximately 6, 12, 20, 30,40, 50, 60, 70, 80, 90, 100, 110, 120, 130 or 140 consecutivenucleotides of either strand of a THBS1 or THBS4 gene.

[0103] As used herein, the term “transfection” means the introduction ofa nucleic acid, e.g., an expression vector, into a recipient cell bynucleic acid-mediated gene transfer. The term “transduction” isgenerally used herein when the transfection with a nucleic acid is byviral delivery of the nucleic acid. “Transformation”, as used herein,refers to a process in which a cell's genotype is changed as a result ofthe cellular uptake of exogenous DNA or RNA, and, for example, thetransformed cell expresses a recombinant form of a polypeptide or, inthe case of anti-sense expression from the transferred gene, theexpression of a naturally-occurring form of the recombinant protein isdisrupted.

[0104] As used herein, the term “transgene” refers to a nucleic acidsequence which has been genetic-engineered into a cell. Daughter cellsderiving from a cell in which a transgene has been introduced are alsosaid to contain the transgene (unless it has been deleted). A transgenecan encode, e.g., a polypeptide, or an antisense transcript, partly orentirely heterologous, i.e., foreign, to the transgenic animal or cellinto which it is introduced, or, is homologous to an endogenous gene ofthe transgenic animal or cell into which it is introduced, but which isdesigned to be inserted, or is inserted, into the animal's genome insuch a way as to alter the genome of the cell into which it is inserted(e.g., it is inserted at a location which differs from that of thenatural gene or its insertion results in a knockout). Alternatively, atransgene can also be present in an episome. A transgene can include oneor more transcriptional regulatory sequence and any other nucleic acid,(e.g. intron), that may be necessary for optimal expression of aselected nucleic acid.

[0105] A “transgenic animal” refers to any animal, preferably anon-human animal, e.g. a mammal, bird or an amphibian, in which one ormore of the cells of the animal contain heterologous nucleic acidintroduced by genetic engineering, such as by transgenic techniques wellknown in the art. The nucleic acid is introduced into the cell, directlyor indirectly by introduction into a precursor of the cell, by way ofdeliberate genetic manipulation, such as by microinjection or byinfection with a recombinant virus. The term genetic manipulation doesnot include classical cross-breeding, or in vitro fertilization, butrather is directed to the introduction of a recombinant DNA molecule.This molecule may be integrated within a chromosome, or it may beextrachromosomally replicating DNA. In the typical transgenic animalsdescribed herein, the transgene causes cells to express a recombinantform of one of a protein, e.g. either agonistic or antagonistic forms.However, transgenic animals in which the recombinant gene is silent arealso contemplated, as for example, the FLP or CRE recombinase dependentconstructs described below. Moreover, “transgenic animal” also includesthose recombinant animals in which gene disruption of one or more genesis caused by human intervention, including both recombination andantisense techniques.

[0106] The term “treatment”, or “treating” as used herein, is defined asthe application or administration of a therapeutic agent to a subject,implementation of lifestyle changes (e.g., changes in diet orenvironment), administration of medication, use of medical devices, suchas, but not limited to, stents, defibrillators, and angioplasty devices,or any combination thereof or, surgical procedures such as percutaneoustransluminal coronary balloon angioplasty (PTCA) or laser angioplasty,defibrillators, implantation of a stent, or other surgical intervention,such as, for example, coronary bypass grafting (CABG), or anycombination thereof, or application or administration of a therapeuticagent to an isolated tissue or cell line from a subject, who has adisease or disorder, a symptom of disease or disorder or apredisposition toward a disease or disorder, with the purpose to cure,heal, alleviate, relieve, alter, remedy, ameliorate, improve or affectthe disease or disorder, the symptoms of the disease or disorder, or thepredisposition toward disease. The medical devices described in themethods of the invention can also be used in combination with amodulator of THBS1 and/or THBS4 gene expression or THBS1 and/or THBS4polypeptide activity. “Modulators of THBS1 and/or THBS4 geneexpression,” as used herein include, for example, THBS1 and/or THBS4nucleic acid molecules, antisense THBS1 and/or THBS4 nucleic acidmolecules, ribozymes, or a small molecules. “Modulators of THBS1 and/orTHBS4 polypeptide activity” include, for example, THBS1 and/orTHBS4-specific antibodies or THBS1 and/or THBS4 proteins orpolypeptides.

[0107] As used herein, the term “vector” refers to a nucleic acidmolecule capable of transporting or replicating another nucleic acid towhich it has been linked. One type of preferred vector is an episome,i.e., a nucleic acid capable of extra-chromosomal replication. Preferredvectors are those capable of autonomous replication and/or expression ofnucleic acids to which they are linked. Vectors capable of directing theexpression of genes to which they are operatively-linked are referred toherein as “expression vectors”. In general, expression vectors ofutility in recombinant DNA techniques are often in the form of“plasmids” which refer generally to circular double stranded DNA circleswhich, in their vector form are not physically linked to the hostchromosome. In the present specification, “plasmid” and “vector” areused interchangeably as the plasmid is the most commonly used form ofvector. However, the invention is intended to include such other formsof expression vectors which serve equivalent functions and which becomeknown in the art subsequently hereto.

[0108] Polymorphisms of the Invention

[0109] The nucleic acid molecules of the present invention includespecific allelic variants of the THBS1 and THBS4 gene, which differ fromthe reference sequences set forth in SEQ ID NOs:1 and 3 respectively, orat least a portion thereof, having a polymorphic region. The preferrednucleic acid molecules of the present invention comprise THBS1 and THBS4sequences having one or more of the polymorphisms shown in Table 1 (SEQID NOs.:5, 6, 7, 8, 9, 10, 11, 12, or 13), and those in linkagedisequilibrium therewith. The invention further comprises isolatednucleic acid molecules complementary to nucleic acid moleculescomprising the polymorphisms of the present invention. Nucleic acidmolecules of the present invention can function as probes or primers,e.g., in methods for determining the allelic identity of a THBS1 orTHBS4 polymorphic region. The nucleic acids of the invention can also beused, singly, or in combination, to determine whether a subject is or isnot at risk of developing a disease associated with a specific allelicvariant of a THBS1 or THBS4 polymorphic region, e.g., a vascular diseaseor disorder. The nucleic acids of the invention can further be used toprepare or express THBS1 or THBS4 polypeptides encoded by specificalleles, such as mutant alleles. Such nucleic acids can be used in genetherapy. Polypeptides encoded by specific THBS1 or THBS4 alleles, suchas mutant THBS1 or THBS4 polypeptides, can also be used in therapy orfor preparing reagents, e.g., antibodies, for detecting THBS1 or THBS4proteins encoded by these alleles. Accordingly, such reagents can beused to detect mutant THBS1 or THBS4 proteins.

[0110] As described herein, allelic variants of human THBS1 or THBS4genes have been identified. The invention is intended to encompass theseallelic variants as well as those in linkage disequilibrium which can beidentified, e.g., according to the methods described herein. “Linkagedisequilibrium” refers to an association between specific alleles at twomarker loci within a particular population. In general, linkagedisequilbrium decreases with an increase in physical distance. Iflinkage disequilbrium exists between two markers, then the genotypicinformation at one marker can be used to make predictions about thegenotype of the second marker.

[0111] The invention also provides isolated nucleic acids comprising atleast one polymorphic region of a THBS1 or THBS4 gene having anucleotide sequence which differs from the reference nucleotide sequenceset forth in SEQ ID NOs:1 and 3, respectively. Preferred nucleic acidshave a variant allele located in the coding region of the THBS1 or THBS4gene. Preferred nucleic acids can also have a polymorphic region in anupstream regulatory element, an exon, or in the 3′ UTR.

[0112] The nucleic acid molecules of the invention can be singlestranded DNA (e.g., an oligonucleotide), double stranded DNA (e.g.,double stranded oligonucleotide) or RNA. Preferred nucleic acidmolecules of the invention can be used as probes or primers. Primers ofthe invention refer to nucleic acids which hybridize to a nucleic acidsequence which is adjacent to the region of interest or which covers theregion of interest and is extended. As used herein, the term“hybridizes” is intended to describe conditions for hybridization andwashing under which nucleotide sequences that are significantlyidentical or homologous to each other remain hybridized to each other.Preferably, the conditions are such that sequences at least about 70%,more preferably at least about 80%, even more preferably at least about85% or 90% identical to each other remain hybridized to each other. Suchstringent conditions vary according to the length of the involvednucleotide sequence but are known to those skilled in the art and can befound or determined based on teachings in Current Protocols in MolecularBiology, Ausubel et al., eds., John Wiley & Sons, Inc. (1995), sections2, 4 and 6. Additional stringent conditions and formulas for determiningsuch conditions can be found in Molecular Cloning: A Laboratory Manual,Sambrook et al., Cold Spring Harbor Press, Cold Spring Harbor, N.Y.(1989), chapters 7, 9 and 11. A preferred, non-limiting example ofstringent hybridization conditions for hybrids that are at leastbasepairs in length includes hybridization in 4×sodium chloride/sodiumcitrate (SSC), at about 65-70° C. (or hybridization in 4×SSC plus 50%formamide at about 42-50° C.) followed by one or more washes in 1×SSC,at about 65-70° C. A preferred, non-limiting example of highly stringenthybridization conditions for such hybrids includes hybridization in1×SSC, at about 65-70° C. (or hybridization in 1×SSC plus 50% formamideat about 42-50° C.) followed by one or more washes in 0.3×SSC, at about65-70° C. A preferred, non-limiting example of reduced stringencyhybridization conditions for such hybrids includes hybridization in4×SSC, at about 50-60° C. (or alternatively hybridization in 6×SSC plus50% formamide at about 40-45° C.) followed by one or more washes in2×SSC, at about 50-60° C. Ranges intermediate to the above-recitedvalues, e.g., at 65-70° C. or at 42-50° C. are also intended to beencompassed by the present invention. SSPE (1×SSPE is 0.15M NaCl, 10 mMNaH₂PO₄, and 1.25 mM EDTA, pH 7.4) can be substituted for SSC (1×SSC is0.15M NaCl and 15 mM sodium citrate) in the hybridization and washbuffers; washes are performed for 15 minutes each after hybridization iscomplete.

[0113] The hybridization temperature for hybrids anticipated to be lessthan 50 base pairs in length should be 5-10° C. less than the meltingtemperature (T_(m)) of the hybrid, where T_(m) is determined accordingto the following equations. For hybrids less than 18 base pairs inlength, T_(m)(° C.)=2(# of A+T bases)+4(# of G+C bases). For hybridsbetween 18 and 49 base pairs in length, T_(m)(°C.)=81.5+16.6(log₁₀[Na⁺])+0.41(%G+C)−(600/N), where N is the number ofbases in the hybrid, and [Na⁺] is the concentration of sodium ions inthe hybridization buffer ([Na⁺] for 1×SSC=0.165 M). It will also berecognized by the skilled practitioner that additional reagents may beadded to hybridization and/or wash buffers to decrease non-specifichybridization of nucleic acid molecules to membranes, for example,nitrocellulose or nylon membranes, including but not limited to blockingagents (e.g., BSA or salmon or herring sperm carrier DNA), detergents(e.g., SDS), chelating agents (e.g., EDTA), Ficoll, PVP and the like.When using nylon membranes, in particular, an additional preferred,non-limiting example of stringent hybridization conditions ishybridization in 0.25-0.5M NaH₂PO₄, 7% SDS at about 65° C., followed byone or more washes at 0.02M NaH₂PO₄, 1% SDS at 65° C., see e.g., Churchand Gilbert (1984) Proc. Natl. Acad. Sci. USA 81:1991-1995, (oralternatively 0.2×SSC, 1% SDS).

[0114] A primer or probe can be used alone in a detection method, or aprimer can be used together with at least one other primer or probe in adetection method. Primers can also be used to amplify at least a portionof a nucleic acid. Probes of the invention refer to nucleic acids whichhybridize to the region of interest and which are not further extended.For example, a probe is a nucleic acid which specifically hybridizes toa polymorphic region of a THBS1 or THBS4 gene, and which byhybridization or absence of hybridization to the DNA of a subject or thetype of hybrid formed will be indicative of the identity of the allelicvariant of the polymorphic region of the THBS1 or THBS4 gene.

[0115] Numerous procedures for determining the nucleotide sequence of anucleic acid molecule, or for determining the presence of mutations innucleic acid molecules include a nucleic acid amplification step, whichcan be carried out by, e.g., polymerase chain reaction (PCR).Accordingly, in one embodiment, the invention provides primers foramplifying portions of a THBS1 or THBS4 gene, such as portions of exonsand/or portions of introns. In a preferred embodiment, the exons and/orsequences adjacent to the exons of the human THBS1 or THBS4 gene will beamplified to, e.g., detect which allelic variant, if any, of apolymorphic region is present in the THBS1 or THBS4 gene of a subject.Preferred primers comprise a nucleotide sequence complementary aspecific allelic variant of a THBS1 or THBS4 polymorphic region and ofsufficient length to selectively hybridize with a THBS1 or THBS4 gene,or a combination thereof. In a preferred embodiment, the primer, e.g., asubstantially purified oligonucleotide, comprises a region having anucleotide sequence which hybridizes under stringent conditions to about6, 8, 10, or 12, preferably 25, 30, 40, 50, or 75 consecutivenucleotides of a THBS1 or THBS4 gene. In an even more preferredembodiment, the primer is capable of hybridizing to a THBS1 or THBS4nucleotide sequence, complements thereof, allelic variants thereof, orcomplements of allelic variants thereof. For example, primers comprisinga nucleotide sequence of at least about 15 consecutive nucleotides, atleast about 25 nucleotides or having from about 15 to about 20nucleotides set forth in any of SEQ ID NOs: 5, 6, 7, 8, 9, 10, 11, 12,or 13, or complements thereof are provided by the invention. Primershaving a sequence of more than about 25 nucleotides are also within thescope of the invention. Preferred primers of the invention are primersthat can be used in PCR for amplifying each of the exons of a THBS1 orTHBS4 gene.

[0116] Primers can be complementary to nucleotide sequences locatedclose to each other or further apart, depending on the use of theamplified DNA. For example, primers can be chosen such that they amplifyDNA fragments of at least about 10 nucleotides or as much as severalkilobases. Preferably, the primers of the invention will hybridizeselectively to THBS1 or THBS4 nucleotide sequences located about 150 toabout 350 nucleotides apart.

[0117] For amplifying at least a portion of a nucleic acid, a forwardprimer (i.e., 5′ primer) and a reverse primer (i.e., 3′ primer) willpreferably be used. Forward and reverse primers hybridize tocomplementary strands of a double stranded nucleic acid, such that uponextension from each primer, a double stranded nucleic acid is amplified.A forward primer can be a primer having a nucleotide sequence or aportion of the nucleotide sequence shown in Table 1 (SEQ ID NOs: 5, 6,7, 8, 9, 10, 11, 12, or 13). A reverse primer can be a primer having anucleotide sequence or a portion of the nucleotide sequence that iscomplementary to a nucleotide sequence shown in Table 1 (SEQ ID NOs: 5,6, 7, 8, 9, 10, 11, 12, or 13).

[0118] Yet other preferred primers of the invention are nucleic acidswhich are capable of selectively hybridizing to an allelic variant of apolymorphic region of a THBS1 or THBS4 gene. Thus, such primers can bespecific for a THBS1 or THBS4 gene sequence, so long as they have anucleotide sequence which is capable of hybridizing to a THBS1 or THBS4gene. Preferred primers are capable of specifically hybridizing to anyof the allelic variants listed in Table 1 (SEQ ID NOs: 5, 6, 7, 8, 9,10, 11, 12, or 13). Such primers can be used, e.g., in sequence specificoligonucleotide priming as described further herein.

[0119] Other preferred primers used in the methods of the invention arenucleic acids which are capable of hybridizing to the reference sequenceof a THBS1 or THBS4 gene, thereby detecting the presence of thereference allele of an allelic variant or the absence of a variantallele of an allelic variant in a THBS1 or THBS4 genes. Such primers canbe used in combination, e.g., primers specific for the variantpolynucleotide of the THBS1 and/or THBS4 genes can be used incombination. The sequences of primers specific for the referencesequences comprising the THBS1 or THBS4 gene will be readily apparent toone of skill in the art.

[0120] The THBS1 or THBS4 nucleic acids of the invention can also beused as probes, e.g., in therapeutic and diagnostic assays. Forinstance, the present invention provides a probe comprising asubstantially purified oligonucleotide, which oligonucleotide comprisesa region having a nucleotide sequence that is capable of hybridizingspecifically to a region of a THBS1 or THBS4 gene which is polymorphic(SEQ ID NOs: 5, 6, 7, 8, 9, 10, 11, 12, or 13). In an even morepreferred embodiment of the invention, the probes are capable ofhybridizing specifically to one allelic variant of a THBS1 or THBS4 genehaving a nucleotide sequence which differs from the nucleotide sequenceset forth in SEQ ID NOs:1 and 3. Such probes can then be used tospecifically detect which allelic variant of a polymorphic region of aTHBS1 or THBS4 gene is present in a subject. The polymorphic region canbe located in the 3′ UTR, 5′ upstream regulatory element, exon, orintron sequences of a THBS1 or THBS4 gene.

[0121] Particularly, preferred probes of the invention have a number ofnucleotides sufficient to allow specific hybridization to the targetnucleotide sequence. Where the target nucleotide sequence is present ina large fragment of DNA, such as a genomic DNA fragment of several tensor hundreds of kilobases, the size of the probe may have to be longer toprovide sufficiently specific hybridization, as compared to a probewhich is used to detect a target sequence which is present in a shorterfragment of DNA. For example, in some diagnostic methods, a portion of aTHBS1 or THBS4 gene may first be amplified and thus isolated from therest of the chromosomal DNA and then hybridized to a probe. In such asituation, a shorter probe will likely provide sufficient specificity ofhybridization. For example, a probe having a nucleotide sequence ofabout 10 nucleotides may be sufficient.

[0122] In preferred embodiments, the probe or primer further comprises alabel attached thereto, which, e.g., is capable of being detected, e.g.the label group is selected from amongst radioisotopes, fluorescentcompounds, enzymes, and enzyme co-factors.

[0123] In a preferred embodiment of the invention, the isolated nucleicacid, which is used, e.g., as a probe or a primer, is modified, so as tobe more stable than naturally occurring nucleotides. Exemplary nucleicacid molecules which are modified include phosphoramidate,phosphothioate and methylphosphonate analogs of DNA (see also U.S. Pat.Nos. 5,176,996; 5,264,564; and 5,256,775).

[0124] The nucleic acids of the invention can also be modified at thebase moiety, sugar moiety, or phosphate backbone, for example, toimprove stability of the molecule. The nucleic acids, e.g., probes orprimers, may include other appended groups such as peptides (e.g., fortargeting host cell receptors in vivo), or agents facilitating transportacross the cell membrane (see, e.g., Letsinger et al., 1989, Proc. Natl.Acad. Sci. U.S.A. 86:6553-6556; Lemaitre et al., 1987, Proc. Natl. Acad.Sci. 84:648-652; PCT Publication No. WO88/09810, published Dec. 15,1988), hybridization-triggered cleavage agents. (See, e.g., Krol et al.,1988, BioTechniques 6:958-976) or intercalating agents (see, e.g., Zon,1988, Pharm. Res. 5:539-549). To this end, the nucleic acid of theinvention may be conjugated to another molecule, e.g., a peptide,hybridization triggered cross-linking agent, transport agent,hybridization-triggered cleavage agent, etc.

[0125] The isolated nucleic acid comprising a THBS1 or THBS4 intronicsequence may comprise at least one modified base moiety which isselected from the group including but not limited to 5-fluorouracil,5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xantine,4-acetylcytidine, 5-(carboxyhydroxymethyl) uracil,5-carboxymethylaminomethyl-2-thiouridine,5-carboxymethylaminomethyluracil, dihydrouracil,beta-D-galactosylqueosine, inosine, N6-isopentenyladenine,1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine,2-methylguanine, 3-methylcytidine, 5-methylcytidine, N6-adenine,7-methylguanine, 5-methylaminomethyluracil,5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine,5′-methoxycarboxymethyluracil, 5-methoxyuracil,2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v),wybutoxosine, pseudouracil, queosine, 2-thiocytidine,5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil,uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v),5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w,and 2,6-diaminopurine.

[0126] The isolated nucleic acid may also comprise at least one modifiedsugar moiety selected from the group including but not limited toarabinose, 2-fluoroarabinose, xylulose, and hexose.

[0127] In yet another embodiment, the nucleic acid comprises at leastone modified phosphate backbone selected from the group consisting of aphosphorothioate, a phosphorodithioate, a phosphoramidothioate, aphosphoramidate, a phosphordiamidate, a methylphosphonate, an alkylphosphotriester, and a formacetal or analog thereof.

[0128] In yet a further embodiment, the nucleic acid is an α-anomericoligonucleotide. An α-anomeric oligonucleotide forms specificdouble-stranded hybrids with complementary RNA in which, contrary to theusual β-units, the strands run parallel to each other (Gautier et al.,1987, Nucl. Acids Res. 15:6625-6641). The oligonucleotide is a2′-0-methylribonucleotide (Inoue et al., 1987, Nucl. Acids Res.15:6131-6148), or a chimeric RNA-DNA analogue (Inoue et al., 1987, FEBSLett. 215:327-330).

[0129] Any nucleic acid fragment of the invention can be preparedaccording to methods well known in the art and described, e.g., inSambrook, J. Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning:A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold SpringHarbor, N.Y. For example, discrete fragments of the DNA can be preparedand cloned using restriction enzymes. Alternatively, discrete fragmentscan be prepared using the Polymerase Chain Reaction (PCR) using primershaving an appropriate sequence.

[0130] Oligonucleotides of the invention may be synthesized by standardmethods known in the art, e.g. by use of an automated DNA synthesizer(such as are commercially available from Biosearch, Applied Biosystems,etc.). As examples, phosphorothioate oligonucleotides may be synthesizedby the method of Stein et al. (1988, Nucl. Acids Res. 16:3209),methylphosphonate oligonucleotides can be prepared by use of controlledpore glass polymer supports (Sarin et al., 1988, Proc. Natl. Acad. Sci.U.S.A. 85:7448-7451), etc.

[0131] The invention also provides vectors and plasmids comprising thenucleic acids of the invention. For example, in one embodiment, theinvention provides a vector comprising at least a portion of the THBS1or THBS4 gene comprising a polymorphic region. Thus, the inventionprovides vectors for expressing at least a portion of the newlyidentified allelic variants of the human THBS1 or THBS4 gene reference,as well as other allelic variants, comprising a nucleotide sequencewhich is different from the nucleotide sequence disclosed in GI 12583762and GI 14916146, respectively. The allelic variants can be expressed ineukaryotic cells, e.g., cells of a subject, e.g., a mammalian subject,or in prokaryotic cells.

[0132] In one embodiment, the vector comprising at least a portion of aTHBS1 or THBS4 allele is introduced into a host cell, such that aprotein encoded by the allele is synthesized. The THBS1 or THBS4 proteinproduced can be used, e.g., for the production of antibodies, which canbe used, e.g., in methods for detecting mutant forms of THBS1 or THBS4.Alternatively, the vector can be used for gene therapy, and be, e.g.,introduced into a subject to produce THBS1 or THBS4 protein. Host cellscomprising a vector having at least a portion of a THBS1 or THBS4 geneare also within the scope of the invention.

[0133] Polypeptides of the Invention

[0134] The present invention provides isolated THBS1 or THBS4polypeptides, such as THBS1 or THBS4 polypeptides which are encoded byspecific allelic variants of THBS1 or THBS4, including those identifiedherein. The amino acid sequences of the THBS1 or THBS4 proteins havebeen deduced. The THBS1 gene encodes a 1,170 amino acid protein and isdescribed in, for example, Wolf, et al. (1990) Genomics Apr;6(4):685-91.The THBS4 gene encodes a 961 amino acid protein and is described in, forexample, Lawler, et al. (1995) J. Biol. Chem. 10;270(6):2809-14). G334u4of the THBS1 gene and G355u2 and G355a11 of the THBS4 gene are missensevariants which result in the change of an amino acid in the amino acidsequence of each gene (see Table 1). G334u3, G334u5, and G334a16 of theTHBS1 gene are silent variants, and G33a18 and G334k2 of the THBS1 geneand TSC0656290 of the THBS4 gene are non-coding variants.

[0135] In one embodiment, the THBS1 or THBS4 polypeptides are isolatedfrom, or otherwise substantially free of other cellular proteins. Theterm “substantially free of other cellular proteins” (also referred toherein as “contaminating proteins”) or “substantially pure or purifiedpreparations” are defined as encompassing preparations of THBS1 or THBS4polypeptides having less than about 20% (by dry weight) contaminatingprotein, and preferably having less than about 5% contaminating protein.It will be appreciated that functional forms of the subject polypeptidescan be prepared, for the first time, as purified preparations by using acloned gene as described herein.

[0136] Preferred THBS1 or THBS4 proteins of the invention have an aminoacid sequence which is at least about 60%, 70%, 80%, 85%, 90%, or 95%identical or homologous to the amino acid sequence of SEQ ID NOs:2 or 4,respectively. Even more preferred THBS1 or THBS4 proteins comprise anamino acid sequence which is at least about 95%, 96%, 97%, 98%, or 99%homologous or identical to the amino acid sequence of SEQ ID NOs:2 or 4,respectively. Such proteins can be recombinant proteins, and can be,e.g., produced in vitro from nucleic acids comprising a specific alleleof a THBS1 or THBS4 polymorphic region. For example, recombinantpolypeptides preferred by the present invention can be encoded by anucleic acid which comprises a sequence which is at least 85% homologousand more preferably 90% homologous and most preferably 95% homologouswith a nucleotide sequence set forth in SEQ ID NOs:1 or 3 and comprisesan allele of a polymorphic region that differs from that set forth inSEQ ID NOs:1 or 3. Polypeptides which are encoded by a nucleic acidcomprising a sequence that is at least about 98-99% homologous with thesequence of SEQ ID NOs:1 or 3 and comprises an allele of a polymorphicregion that differs from that set forth in SEQ ID NOs:1 or 3 are alsowithin the scope of the invention.

[0137] In a preferred embodiment, a THBS1 or THBS4 protein of thepresent invention is a mammalian THBS1 or THBS4 protein. In an even morepreferred embodiment, the THBS1 or THBS4 protein is a human protein.

[0138] The invention also provides peptides that preferably are capableof functioning in one of either role of an agonist or antagonist of atleast one biological activity of a wild-type (“normal”) THBS1 or THBS4protein of the appended sequence listing. The term “evolutionarilyrelated to,” with respect to amino acid sequences of THBS1 or THBS4proteins, refers to both polypeptides having amino acid sequences foundin human populations, and also to artificially produced mutationalvariants of human THBS1 or THBS4 polypeptides which are derived, forexample, by combinatorial mutagenesis.

[0139] Full length proteins or fragments corresponding to one or moreparticular motifs and/or domains or to arbitrary sizes, for example, atleast 5, 10, 25, 50, 75 and 100, amino acids in length of THBS1 or THBS4protein are within the scope of the present invention.

[0140] Isolated THBS1 or THBS4 peptides or polypeptides can be obtainedby screening peptides recombinantly produced from the correspondingfragment of the nucleic acid encoding such peptides. In addition, suchpeptides and polypeptides can be chemically synthesized using techniquesknown in the art such as conventional Merrifield solid phase f-Moc ort-Boc chemistry. For example, a THBS1 or THBS4 peptide or polypeptide ofthe present invention may be arbitrarily divided into fragments ofdesired length with no overlap of the fragments, or preferably dividedinto overlapping fragments of a desired length. The fragments can beproduced (recombinantly or by chemical synthesis) and tested to identifythose peptides or polypeptides which can function as either agonists orantagonists of a wild-type (e.g., “normal”) THBS1 or THBS4 protein.

[0141] In general, peptides and polypeptides referred to herein ashaving an activity (e.g., are “bioactive”) of a THBS1 or THBS4 proteinare defined as peptides and polypeptides which mimic or antagonize allor a portion of the biological/biochemical activities of a THBS1 orTHBS4 protein having SEQ ID NO:2 or SEQ ID NO:4, respectively, such asthe ability to bind ligands. Other biological activities of the subjectTHBS1 or THBS4 proteins are described herein or will be reasonablyapparent to those skilled in the art. According to the presentinvention, a peptide or polypeptide has biological activity if it is aspecific agonist or antagonist of a naturally-occurring form of a THBS1or THBS4 protein.

[0142] Assays for determining whether a THBS1 or THBS4 protein orvariant thereof, has one or more biological activities are well known inthe art.

[0143] Other preferred proteins of the invention are those encoded bythe nucleic acids set forth in the section pertaining to nucleic acidsof the invention. In particular, the invention provides fusion proteins,e.g., THBS1- or THBS4-immunoglobulin fusion proteins. Such fusionproteins can provide, e.g., enhanced stability and solubility of THBS1or THBS4 proteins and may thus be useful in therapy. Fusion proteins canalso be used to produce an immunogenic fragment of a THBS1 or THBS4protein. For example, the VP6 capsid protein of rotavirus can be used asan immunologic carrier protein for portions of the THBS1 or THBS4polypeptide, either in the monomeric form or in the form of a viralparticle. The nucleic acid sequences corresponding to the portion of asubject THBS1 or THBS4 protein to which antibodies are to be raised canbe incorporated into a fusion gene construct which includes codingsequences for a late vaccinia virus structural protein to produce a setof recombinant viruses expressing fusion proteins comprising THBS1 orTHBS4 epitopes as part of the virion. It has been demonstrated with theuse of immunogenic fusion proteins utilizing the Hepatitis B surfaceantigen fusion proteins that recombinant Hepatitis B virions can beutilized in this role as well. Similarly, chimeric constructs coding forfusion proteins containing a portion of a THBS1 or THBS4 protein and thepoliovirus capsid protein can be created to enhance immunogenicity ofthe set of polypeptide antigens (see, for example, EP Publication No:0259149; and Evans et al. (1989) Nature 339:385; Huang et al. (1988) J.Virol. 62:3855; and Schlienger et al. (1992) J. Virol. 66:2).

[0144] The Multiple antigen peptide system for peptide-basedimmunization can also be utilized to generate an immunogen, wherein adesired portion of a THBS1 or THBS4 polypeptide is obtained directlyfrom organo-chemical synthesis of the peptide onto an oligomericbranching lysine core (see, for example, Posnett et al. (1988) JBC263:1719 and Nardelli et al. (1992) J. Immunol. 148:914). Antigenicdeterminants of THBS1 or THBS4 proteins can also be expressed andpresented by bacterial cells.

[0145] Fusion proteins can also facilitate the expression of proteinsincluding the THBS1 or THBS4 polypeptides of the present invention. Forexample, THBS1 or THBS4 polypeptides can be generated asglutathione-S-transferase (GST-fusion) proteins. Such GST-fusionproteins can be easily purified, as for example by the use ofglutathione-derivatized matrices (see, for example, Current Protocols inMolecular Biology, eds. Ausubel et al. (N.Y.: John Wiley & Sons, 1991))and used subsequently to yield purified THBS1 or THBS4 polypeptides.

[0146] The present invention further pertains to methods of producingthe subject THBS1 or THBS4 polypeptides. For example, a host celltransfected with a nucleic acid vector directing expression of anucleotide sequence encoding the subject polypeptides can be culturedunder appropriate conditions to allow expression of the peptide tooccur. Suitable media for cell culture are well known in the art. Therecombinant THBS1 or THBS4 polypeptide can be isolated from cell culturemedium, host cells, or both using techniques known in the art forpurifying proteins including ion-exchange chromatography, gel filtrationchromatography, ultrafiltration, electrophoresis, and immunoaffinitypurification with antibodies specific for such peptide. In a preferredembodiment, the recombinant THBS1 or THBS4 polypeptide is a fusionprotein containing a domain which facilitates its purification, such asGST fusion protein.

[0147] Moreover, it will be generally appreciated that, under certaincircumstances, it may be advantageous to provide homologs of one of thesubject THBS1 or THBS4 polypeptides which function in a limited capacityas one of either a THBS1 or THBS4 agonist (mimetic) or a THBS1 or THBS4antagonist, in order to promote or inhibit only a subset of thebiological activities of the naturally-occurring form of the protein.Thus, specific biological effects can be elicited by treatment with ahomolog of limited function, and with fewer side effects relative totreatment with agonists or antagonists which are directed to all of thebiological activities of naturally occurring forms of THBS1 or THBS4proteins.

[0148] Homologs of each of the subject THBS1 or THBS4 proteins can begenerated by mutagenesis, such as by discrete point mutation(s), and/orby truncation. For instance, mutation can give rise to homologs whichretain substantially the same, or merely a subset, of the biologicalactivity of the THBS1 or THBS4 polypeptide from which it was derived.Alternatively, antagonistic forms of the protein can be generated whichare able to inhibit the function of the naturally occurring form of theprotein, such as by competitively binding to a THBS1 or THBS4 receptor.

[0149] The recombinant THBS1 or THBS4 polypeptides of the presentinvention also include homologs of THBS1 or THBS4 polypeptides whichdiffer from the THBS1 or THBS4 protein having SEQ ID NO:2 or SEQ IDNO:4, respectively, such as versions of the protein which are resistantto proteolytic cleavage, as for example, due to mutations which alterubiquitination or other enzymatic targeting associated with the protein.

[0150] THBS1 or THBS4 polypeptides may also be chemically modified tocreate THBS1 or THBS4 derivatives by forming covalent or aggregateconjugates with other chemical moieties, such as glycosyl groups,lipids, phosphate, acetyl groups and the like. Covalent derivatives ofTHBS1 or THBS4 proteins can be prepared by linking the chemical moietiesto functional groups on amino acid side-chains of the protein or at theN-terminus or at the C-terminus of the polypeptide.

[0151] Modification of the structure of the subject THBS1 or THBS4polypeptides can be for such purposes as enhancing therapeutic orprophylactic efficacy, stability (e.g., ex vivo shelf life andresistance to proteolytic degradation), or post-translationalmodifications (e.g., to alter phosphorylation pattern of protein). Suchmodified peptides, when designed to retain at least one activity of thenaturally-occurring form of the protein, or to produce specificantagonists thereof, are considered functional equivalents of the THBS1or THBS4 polypeptides described in more detail herein. Such modifiedpeptides can be produced, for instance, by amino acid substitution,deletion, or addition. The substitutional variant may be a substitutedconserved amino acid or a substituted non-conserved amino acid.

[0152] For example, it is reasonable to expect that an isolatedreplacement of a leucine with an isoleucine or valine, an aspartate witha glutamate, a threonine with a serine, or a similar replacement of anamino acid with a structurally related amino acid (i.e., isostericand/or isoelectric mutations) will not have a major effect on thebiological activity of the resulting molecule. Conservative replacementsare those that take place within a family of amino acids that arerelated in their side chains. Genetically encoded amino acids can bedivided into four families: (1) acidic=aspartate, glutamate; (2)basic=lysine, arginine, histidine; (3) nonpolar=alanine, valine,leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and(4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine,threonine, tyrosine. In similar fashion, the amino acid repertoire canbe grouped as (1) acidic=aspartate, glutamate; (2) basic=lysine,arginine histidine, (3) aliphatic=glycine, alanine, valine, leucine,isoleucine, serine, threonine, with serine and threonine optionally begrouped separately as aliphatic-hydroxyl; (4) aromatic=phenylalanine,tyrosine, tryptophan; (5) amide=asparagine, glutamine; and (6)sulfur-containing=cysteine and methionine. (see, for example,Biochemistry, 2^(nd) ed., Ed. by L. Stryer, W H Freeman and Co.: 1981).Whether a change in the amino acid sequence of a peptide results in afunctional THBS1 or THBS4 homolog (e.g., functional in the sense thatthe resulting polypeptide mimics or antagonizes the wild-type form) canbe readily determined by assessing the ability of the variant peptide toproduce a response in cells in a fashion similar to the wild-typeprotein, or competitively inhibit such a response. Polypeptides in whichmore than one replacement has taken place can readily be tested in thesame manner.

[0153] Methods

[0154] The invention further provides predictive medicine methods, whichare based, at least in part, on the discovery of THBS1 or THBS4polymorphic regions which are associated with specific physiologicalstates and/or diseases or disorders, e.g., vascular diseases ordisorders such as CAD and MI. These methods can be used alone, or incombination with other predictive medicine methods, including theidentification and analysis of known risk factors associated withvascular disease, e.g., phenotypic factors such as, for example,obesity, diabetes, and family history.

[0155] For example, information obtained using the diagnostic assaysdescribed herein (singly or in combination with information of anothergenetic defect which contributes to the same disease, e.g., a vasculardisease or disorder) is useful for diagnosing or confirming that asubject has an allele of a polymorphic region which is associated with aparticular disease or disorder, e.g., a vascular disease or disorder, ora combination of alleles which are associated with a particular diseaseor disorder, e.g., one or more of the variant alleles at nucleotidepositions 55322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ IDNO:1, and/or nucleotide positions 105290, 118019, and/or 89835 of SEQ IDNO:3, or the complement thereof. Moreover, the information obtainedusing the diagnostic assays described herein, singly or in combinationwith information of another genetic defect which contributes to the samedisease, e.g., a vascular disease or disorder, can be used to predictwhether or not a subject will benefit from further diagnostic evaluationfor a vascular disease or disorder. Such further diagnostic evaluationincludes, but is not limited to, cardiovascular imaging, such asangiography, cardiac ultrasound, coronary angiogram, magnetic resonanceimagery, nuclear imaging, CT scan, myocardial perfusion imagery, orelectrocardiogram, genetic analysis, e.g., identification of additionalpolymorphisms e.g., which contribute to the same disease, familialhealth history analysis, lifestyle analysis, or exercise stress tests,either alone or in combination. Furthermore, the diagnostic informationobtained using the diagnostic assays described herein (singly or incombination with information of another genetic defect which contributesto the same disease, e.g., a vascular disease or disorder), may be usedto identify which subject will benefit from a particular clinical courseof therapy useful for preventing, treating, ameliorating, or prolongingonset of the particular vascular disease or disorder in the particularsubject. Clinical courses of therapy include, but are not limited to,administration of medication, non-surgical intervention, surgicalprocedures such as percutaneous transluminal coronary angioplasty, laserangioplasty, implantation of a stent, coronary bypass grafting,implantation of a defibrillator, implantation of a pacemaker, and anycombination thereof, and use of surgical and non-surgical medicaldevices used in the treatment of vascular disease, such as, for example,a defibrillator, a stent, a device used in coronary revascularization, apacemaker, and any combination thereof. Medical devices may also be usedin combination with a modulator of THBS1 or THBS4 gene expression orTHBS1 or THBS4 polypeptide activity.

[0156] Alternatively, the information, singly, or, preferably, incombination with information of another genetic defect which contributesto the same disease, e.g., a vascular disease or disorder, can be usedprognostically for predicting whether a non-symptomatic subject islikely to develop a disease or condition which is associated with one ormore specific alleles of THBS1 or THBS4 polymorphic regions in asubject. Based on the prognostic information, a health care provider canrecommend a particular further diagnostic evaluation which will benefitthe subject, or a particular clinical course of therapy, as describedabove.

[0157] In addition, knowledge of the identity of one or more particularTHBS1 or THBS4 alleles in a subject (the THBS1 or THBS4 geneticprofile), preferably, one or more of the alleles at nucleotide positions55322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ ID NO:1, and/ornucleotide positions 105290, 118019, and/or 89835 of SEQ ID NO:3, or thecomplement thereof, allows customization of further diagnosticevaluation and/or a clinical course of therapy for a particular disease.For example, a subject's THBS1 or THBS4 genetic profile or the geneticprofile of a disease or disorder associated with a specific allele of aTHBS1 or THBS4 polymorphic region, e.g., a vascular disease or disorder,can enable a health care provider: 1) to more efficiently andcost-effectively identify means for further diagnostic evaluation,including, but not limited to, further genetic analysis, familial healthhistory analysis, or use of vascular imaging devices or procedures; 2)to more effectively prescribe a drug that will address the molecularbasis of the disease or condition; 3) to more efficiently andcost-effectively identify an appropriate clinical course of therapy,including, but not limited to, lifestyle changes, medications, surgicalor non-surgical medical devices, surgical or non-surgical interventionor procedures, or any combination thereof; and 4) to better determinethe appropriate dosage of a particular drug or duration of a particularcourse of clinical therapy. For example, the expression level of THBS1or THBS4 proteins, alone or in conjunction with the expression level ofother genes known to contribute to the same disease, can be measured inmany subjects at various stages of the disease to generate atranscriptional or expression profile of the disease. Expressionpatterns of individual subjects can then be compared to the expressionprofile of the disease to determine the appropriate drug, dose toadminister to the subject, or course of clinical therapy.

[0158] The ability to target populations expected to show the highestclinical benefit, based on the THBS1 or THBS4 or disease geneticprofile, can enable: 1) the repositioning of marketed drugs, medicaldevices and surgical procedures for use in treating, preventing, orameliorating vascular diseases or disorders, or diagnostics, such asvascular imaging devices or procedures, with disappointing marketresults; 2) the rescue of drug candidates whose clinical development hasbeen discontinued as a result of safety or efficacy limitations, whichare subject subgroup-specific; 3) an accelerated and less costlydevelopment for drug candidates and more optimal drug labeling (e.g.,since the use of THBS1 or THBS4 as a marker is useful for optimizingeffective dose); and 4) an accelerated, less costly, and more effectiveselection of a particular course of clinical therapy suited to aparticular subject.

[0159] These and other methods are described in further detail in thefollowing sections.

[0160] A. Prognostic and Diagnostic Assays

[0161] The present methods provide means for determining if a subjecthas or is or is not at risk of developing a disease, condition ordisorder that is associated a specific THBS1 or THBS4 allele orcombinations thereof, e.g., a vascular disease or a disease or disorderresulting therefrom.

[0162] The present invention provides methods for determining themolecular structure of a THBS1 or THBS4 gene, such as a human THBS1 orTHBS4 gene, or a portion thereof. In one embodiment, determining themolecular structure of at least a portion of a THBS1 or THBS4 genecomprises determining the identity of the allelic variant of at leastone polymorphic region of a THBS1 or THBS4 gene (determining thepresence or absence of one or more of the allelic variants, or theircomplements, of SEQ ID NOs: 5, 6, 7, 8, 9, 10, 11, 12, or 13). Apolymorphic region of a THBS1 or THBS4 gene can be located in an exon,an intron, at an intron/exon border, or in the 5′ upstream regulatoryelement of the THBS1 or THBS4 gene.

[0163] The invention provides methods for determining whether a subjecthas or is at risk of developing, a disease or disorder associated with aspecific allelic variant of a polymorphic region of a THBS1 or THBS4gene. Such diseases can be associated with aberrant THBS1 or THBS4activity, e.g., a vascular disease or disorder.

[0164] Analysis of one or more THBS1 or THBS4 polymorphic regions in asubject can be useful for predicting whether a subject has or is likelyto develop a vascular disease or disorder, e.g., CAD, MI,atherosclerosis, ischemia, stroke, peripheral vascular diseases, venousthromboembolism and pulmonary embolism.

[0165] In preferred embodiments, the methods of the invention can becharacterized as comprising detecting, in a sample of cells from thesubject, the presence or absence of a specific allelic variant of one ormore polymorphic regions of a THBS1 or THBS4 gene. The allelicdifferences can be: (i) a difference in the identity of at least onenucleotide or (ii) a difference in the number of nucleotides, whichdifference can be a single nucleotide or several nucleotides. Theinvention also provides methods for detecting differences in THBS1 orTHBS4 genes such as chromosomal rearrangements, e.g., chromosomaldislocation. The invention can also be used in prenatal diagnostics.

[0166] A preferred detection method is allele specific hybridizationusing probes overlapping the polymorphic site and having about 5, 10,20, 25, or 30 nucleotides around the polymorphic region. In a preferredembodiment of the invention, several probes capable of hybridizingspecifically to allelic variants are attached to a solid phase support,e.g., a “chip”. Oligonucleotides can be bound to a solid support by avariety of processes, including lithography. For example a chip can holdup to 250,000 oligonucleotides (GeneChip, Affymetrix). Mutationdetection analysis using these chips comprising oligonucleotides, alsotermed “DNA probe arrays” is described e.g., in Cronin et al. (1996)Human Mutation 7:244. In one embodiment, a chip comprises all theallelic variants of at least one polymorphic region of a gene. The solidphase support is then contacted with a test nucleic acid andhybridization to the specific probes is detected. Accordingly, theidentity of numerous allelic variants of one or more genes can beidentified in a simple hybridization experiment. For example, theidentity of the allelic variant of the nucleotide polymorphism in the 5′upstream regulatory element can be determined in a single hybridizationexperiment.

[0167] In other detection methods, it is necessary to first amplify atleast a portion of a THBS1 or THBS4 gene prior to identifying theallelic variant. Amplification can be performed, e.g., by PCR and/or LCR(see Wu and Wallace, (1989) Genomics 4:560), according to methods knownin the art. In one embodiment, genomic DNA of a cell is exposed to twoPCR primers and amplification for a number of cycles sufficient toproduce the required amount of amplified DNA. In preferred embodiments,the primers are located between 150 and 350 base pairs apart.

[0168] Alternative amplification methods include: self sustainedsequence replication (Guatelli, J. C. et al., 1990, Proc. Natl. Acad.Sci. USA 87:1874-1878), transcriptional amplification system (Kwoh, D.Y. et al., 1989, Proc. Natl. Acad. Sci. USA 86:1173-1177), Q-BetaReplicase (Lizardi, P. M. et al., 1988, Bio/Technology 6:1197), andself-sustained sequence replication (Guatelli et al., (1989) Proc. Nat.Acad. Sci. 87:1874), and nucleic acid based sequence amplification(NABSA), or any other nucleic acid amplification method, followed by thedetection of the amplified molecules using techniques well known tothose of skill in the art. These detection schemes are especially usefulfor the detection of nucleic acid molecules if such molecules arepresent in very low numbers.

[0169] In one embodiment, any of a variety of sequencing reactions knownin the art can be used to directly sequence at least a portion of aTHBS1 or THBS4 gene and detect allelic variants, e.g., mutations, bycomparing the sequence of the sample sequence with the correspondingreference (control) sequence. Exemplary sequencing reactions includethose based on techniques developed by Maxam and Gilbert (Proc. NatlAcad Sci USA (1977) 74:560) or Sanger (Sanger et al. (1977) Proc. Nat.Acad. Sci 74:5463). It is also contemplated that any of a variety ofautomated sequencing procedures may be utilized when performing thesubject assays (Biotechniques (1995) 19:448), including sequencing bymass spectrometry (see, for example, U.S. Pat. No. 5,547,835 andinternational patent application Publication Number WO 94/16101,entitled DNA Sequencing by Mass Spectrometry by H. Köster; U.S. Pat. No.5,547,835 and international patent application Publication Number WO94/21822 entitled “DNA Sequencing by Mass Spectrometry Via ExonucleaseDegradation” by H. Köster), and U.S. Pat. No. 5,605,798 andInternational Patent Application No. PCT/US96/03651 entitled DNADiagnostics Based on Mass Spectrometry by H. Köster; Cohen et al. (1996)Adv Chromatogr 36:127-162; and Griffin et al. (1993) Appl BiochemBiotechnol 38:147-159). It will be evident to one skilled in the artthat, for certain embodiments, the occurrence of only one, two or threeof the nucleic acid bases need be determined in the sequencing reaction.For instance, A-track or the like, e.g., where only one nucleotide isdetected, can be carried out.

[0170] Yet other sequencing methods are disclosed, e.g., in U.S. Pat.No. 5,580,732 entitled “Method of DNA sequencing employing a mixedDNA-polymer chain probe” and U.S. Pat. No. 5,571,676 entitled “Methodfor mismatch-directed in vitro DNA sequencing”.

[0171] In some cases, the presence of a specific allele of a THBS1 orTHBS4 gene in DNA from a subject can be shown by restriction enzymeanalysis. For example, a specific nucleotide polymorphism can result ina nucleotide sequence comprising a restriction site which is absent fromthe nucleotide sequence of another allelic variant.

[0172] In a further embodiment, protection from cleavage agents (such asa nuclease, hydroxylamine or osmium tetroxide and with piperidine) canbe used to detect mismatched bases in RNA/RNA DNA/DNA, or RNA/DNAheteroduplexes (Myers, et al. (1985) Science 230:1242). In general, thetechnique of “mismatch cleavage” starts by providing heteroduplexesformed by hybridizing a control nucleic acid, which is optionallylabeled, e.g., RNA or DNA, comprising a nucleotide sequence of a THBS1or THBS4 allelic variant with a sample nucleic acid, e.g., RNA or DNA,obtained from a tissue sample. The double-stranded duplexes are treatedwith an agent which cleaves single-stranded regions of the duplex suchas duplexes formed based on basepair mismatches between the control andsample strands. For instance, RNA/DNA duplexes can be treated with RNaseand DNA/DNA hybrids treated with S1 nuclease to enzymatically digest themismatched regions. In other embodiments, either DNA/DNA or RNA/DNAduplexes can be treated with hydroxylamine or osmium tetroxide and withpiperidine in order to digest mismatched regions. After digestion of themismatched regions, the resulting material is then separated by size ondenaturing polyacrylamide gels to determine whether the control andsample nucleic acids have an identical nucleotide sequence or in whichnucleotides they are different. See, for example, Cotton et al. (1988)Proc. Natl. Acad Sci USA 85:4397; Saleeba et al (1992) Methods Enzymol.217:286-295. In a preferred embodiment, the control or sample nucleicacid is labeled for detection.

[0173] In another embodiment, an allelic variant can be identified bydenaturing high-performance liquid chromatography (DHPLC) (Oefner andUnderhill, (1995) Am. J. Human Gen. 57:Suppl. A266). DHPLC usesreverse-phase ion-pairing chromatography to detect the heteroduplexesthat are generated during amplification of PCR fragments fromindividuals who are heterozygous at a particular nucleotide locus withinthat fragment (Oefner and Underhill (1995) Am. J. Human Gen. 57:Suppl.A266). In general, PCR products are produced using PCR primers flankingthe DNA of interest. DHPLC analysis is carried out and the resultingchromatograms are analyzed to identify base pair alterations ordeletions based on specific chromatographic profiles (see O'Donovan etal. (1998) Genomics 52:44-49).

[0174] In other embodiments, alterations in electrophoretic mobility isused to identify the type of THBS1 or THBS4 allelic variant. Forexample, single strand conformation polymorphism (SSCP) may be used todetect differences in electrophoretic mobility between mutant and wildtype nucleic acids (Orita et al. (1989) Proc Natl. Acad. Sci USA86:2766; see also Cotton (1993) Mutat Res 285:125-144; and Hayashi(1992) Genet Anal Tech Appl 9:73-79). Single-stranded DNA fragments ofsample and control nucleic acids are denatured and allowed to renature.The secondary structure of single-stranded nucleic acids variesaccording to sequence, the resulting alteration in electrophoreticmobility enables the detection of even a single base change. The DNAfragments may be labeled or detected with labeled probes. Thesensitivity of the assay may be enhanced by using RNA (rather than DNA),in which the secondary structure is more sensitive to a change insequence. In another preferred embodiment, the subject method utilizesheteroduplex analysis to separate double stranded heteroduplex moleculeson the basis of changes in electrophoretic mobility (Keen et al. (1991)Trends Genet 7:5).

[0175] In yet another embodiment, the identity of an allelic variant ofa polymorphic region is obtained by analyzing the movement of a nucleicacid comprising the polymorphic region in polyacrylamide gels containinga gradient of denaturant is assayed using denaturing gradient gelelectrophoresis (DGGE) (Myers et al. (1985) Nature 313:495). When DGGEis used as the method of analysis, DNA will be modified to insure thatit does not completely denature, for example by adding a GC clamp ofapproximately 40 bp of high-melting GC-rich DNA by PCR. In a furtherembodiment, a temperature gradient is used in place of a denaturingagent gradient to identify differences in the mobility of control andsample DNA (Rosenbaum and Reissner (1987) Biophys Chem 265:1275).

[0176] Examples of techniques for detecting differences of at least onenucleotide between 2 nucleic acids include, but are not limited to,selective oligonucleotide hybridization, selective amplification, orselective primer extension. For example, oligonucleotide probes may beprepared in which the known polymorphic nucleotide is placed centrally(allele-specific probes) and then hybridized to target DNA underconditions which permit hybridization only if a perfect match is found(Saiki et al. (1986) Nature 324:163); Saiki et al (1989) Proc. Natl.Acad. Sci USA 86:6230; and Wallace et al. (1979) Nucl. Acids Res.6:3543). Such allele specific oligonucleotide hybridization techniquesmay be used for the simultaneous detection of several nucleotide changesin different polylmorphic regions of THBS1 or THBS4. For example,oligonucleotides having nucleotide sequences of specific allelicvariants are attached to a hybridizing membrane and this membrane isthen hybridized with labeled sample nucleic acid. Analysis of thehybridization signal will then reveal the identity of the nucleotides ofthe sample nucleic acid.

[0177] Alternatively, allele specific amplification technology whichdepends on selective PCR amplification may be used in conjunction withthe instant invention. Oligonucleotides used as primers for specificamplification may carry the allelic variant of interest in the center ofthe molecule (so that amplification depends on differentialhybridization) (Gibbs et al. (1989) Nucleic Acids Res. 17:2437-2448) orat the extreme 3′ end of one primer where, under appropriate conditions,mismatch can prevent, or reduce polymerase extension (Prossner (1993)Tibtech 11:238; Newton et al. (1989) Nucl. Acids Res. 17:2503). Thistechnique is also termed “PROBE” for Probe Oligo Base Extension. Inaddition it may be desirable to introduce a novel restriction site inthe region of the mutation to create cleavage-based detection (Gaspariniet al. (1992) Mol. Cell Probes 6:1).

[0178] In another embodiment, identification of the allelic variant iscarried out using an oligonucleotide ligation assay (OLA), as described,e.g., in U.S. Pat. No. 4,998,617 and in Landegren, U. et al., (1988)Science 241:1077-1080. The OLA protocol uses two oligonucleotides whichare designed to be capable of hybridizing to abutting sequences of asingle strand of a target. One of the oligonucleotides is linked to aseparation marker, e.g,. biotinylated, and the other is detectablylabeled. If the precise complementary sequence is found in a targetmolecule, the oligonucleotides will hybridize such that their terminiabut, and create a ligation substrate. Ligation then permits the labeledoligonucleotide to be recovered using avidin, or another biotin ligand.Nickerson, D. A. et al. have described a nucleic acid detection assaythat combines attributes of PCR and OLA (Nickerson, D. A. et al., (1990)Proc. Natl. Acad. Sci. (U.S.A.) 87:8923-8927. In this method, PCR isused to achieve the exponential amplification of target DNA, which isthen detected using OLA.

[0179] Several techniques based on this OLA method have been developedand can be used to detect specific allelic variants of a polymorphicregion of a THBS1 or THBS4 gene. For example, U.S. Pat. No. 5,593,826discloses an OLA using an oligonucleotide having 3′-amino group and a5′-phosphorylated oligonucleotide to form a conjugate having aphosphoramidate linkage. In another variation of OLA described in Tobeet al. ((1996) Nucleic Acids Res 24: 3728), OLA combined with PCRpermits typing of two alleles in a single microtiter well. By markingeach of the allele-specific primers with a unique hapten, i.e.digoxigenin and fluorescein, each OLA reaction can be detected by usinghapten specific antibodies that are labeled with different enzymereporters, alkaline phosphatase or horseradish peroxidase. This systempermits the detection of the two alleles using a high throughput formatthat leads to the production of two different colors.

[0180] The invention further provides methods for detecting singlenucleotide polymorphisms in a THBS1 or THBS4 gene. Because singlenucleotide polymorphisms constitute sites of variation flanked byregions of invariant sequence, their analysis requires no more than thedetermination of the identity of the single nucleotide present at thesite of variation and it is unnecessary to determine a complete genesequence for each subject. Several methods have been developed tofacilitate the analysis of such single nucleotide polymorphisms.

[0181] In one embodiment, the single base polymorphism can be detectedby using a specialized exonuclease-resistant nucleotide, as disclosed,e.g., in Mundy, C. R. (U.S. Pat. No. 4,656,127). According to themethod, a primer complementary to the allelic sequence immediately 3′ tothe polymorphic site is permitted to hybridize to a target moleculeobtained from a particular animal or human. If the polymorphic site onthe target molecule contains a nucleotide that is complementary to theparticular exonuclease-resistant nucleotide derivative present, thenthat derivative will be incorporated onto the end of the hybridizedprimer. Such incorporation renders the primer resistant to exonuclease,and thereby permits its detection. Since the identity of theexonuclease-resistant derivative of the sample is known, a finding thatthe primer has become resistant to exonucleases reveals that thenucleotide present in the polymorphic site of the target molecule wascomplementary to that of the nucleotide derivative used in the reaction.This method has the advantage that it does not require the determinationof large amounts of extraneous sequence data.

[0182] In another embodiment of the invention, a solution-based methodis used for determining the identity of the nucleotide of a polymorphicsite (Cohen, D. et al. (French Patent 2,650,840; PCT Application No.WO91/02087). As in the Mundy method of U.S. Pat. No. 4,656,127, a primeris employed that is complementary to allelic sequences immediately 3′ toa polymorphic site. The method determines the identity of the nucleotideof that site using labeled dideoxynucleotide derivatives, which, ifcomplementary to the nucleotide of the polymorphic site will becomeincorporated onto the terminus of the primer.

[0183] An alternative method, known as Genetic Bit Analysis or GBA™ isdescribed by Goelet, P. et al. (PCT Application No. 92/15712). Themethod of Goelet, P. et al. uses mixtures of labeled terminators and aprimer that is complementary to the sequence 3′ to a polymorphic site.The labeled terminator that is incorporated is thus determined by, andcomplementary to, the nucleotide present in the polymorphic site of thetarget molecule being evaluated. In contrast to the method of Cohen etal. (French Patent 2,650,840; PCT Appln. No. WO91/02087) the method ofGoelet, P. et al. is preferably a heterogeneous phase assay, in whichthe primer or the target molecule is immobilized to a solid phase.

[0184] Several primer-guided nucleotide incorporation procedures forassaying polymorphic sites in DNA have been described (Komher, J. S. etal., Nucl. Acids. Res. 17:7779-7784 (1989); Sokolov, B. P., Nucl. AcidsRes. 18:3671 (1990); Syvanen, A. -C., et al., Genomics 8:684-692 (1990);Kuppuswamy, M. N. et al., Proc. Natl. Acad. Sci. (U.S.A.) 88:1143-1147(1991); Prezant, T. R. et al., Hum. Mutat. 1: 159-164 (1992); Ugozzoli,L. et al., GATA 9:107-112 (1992); Nyren, P. et al., Anal. Biochem.208:171-175 (1993)). These methods differ from GBA™ in that they allrely on the incorporation of labeled deoxynucleotides to discriminatebetween bases at a polymorphic site. In such a format, since the signalis proportional to the number of deoxynucleotides incorporated,polymorphisms that occur in runs of the same nucleotide can result insignals that are proportional to the length of the run (Syvanen, A. -C.,et al., Amer. J. Hum. Genet. 52:46-59 (1993)).

[0185] For determining the identity of the allelic variant of apolymorphic region located in 110 the coding region of a THBS1 or THBS4gene, yet other methods than those described above can be used. Forexample, identification of an allelic variant which encodes a mutatedTHBS1 or THBS4 protein can be performed by using an antibodyspecifically recognizing the mutant protein in, e.g.,immunohistochemistry or immunoprecipitation. Antibodies to wild-typeTHBS1 proteins are described in, for example, Lawler, et al. (1995) J.Biol. Chem. 270(6):2809-14. Antibodies to wild-type THBS4 proteins aredescribed in, for example, Dixit, et al. (1985) Proc. Natl. Acad. Sci.82(10):3472-6. Other antibodies to wild-type THBS1 or THBS4 or mutatedforms of THBS1 or THBS4 proteins can be prepared according to methodsknown in the art.

[0186] Alternatively, one can also measure an activity of a THBS1 orTHBS4 protein, such as binding to a THBS1 or THBS4 ligand. Bindingassays are known in the art and involve, e.g., obtaining cells from asubject, and performing binding experiments with a labeled lipid, todetermine whether binding to the mutated form of the protein differsfrom binding to the wild-type of the protein.

[0187] Antibodies directed against reference or mutant THBS1 or THBS4polypeptides or allelic variant thereof, which are discussed above, mayalso be used in disease diagnostics and prognostics. Such diagnosticmethods, may be used to detect abnormalities in the level of THBS1 orTHBS4 polypeptide expression, or abnormalities in the structure and/ortissue, cellular, or subcellular location of a THBS1 or THBS4polypeptide. Structural differences may include, for example,differences in the size, electronegativity, or antigenicity of themutant THBS1 or THBS4 polypeptide relative to the normal THBS1 or THBS4polypeptide. Protein from the tissue or cell type to be analyzed mayeasily be detected or isolated using techniques which are well known toone of skill in the art, including but not limited to Western blotanalysis. For a detailed explanation of methods for carrying out Westernblot analysis, see Sambrook et al, 1989, supra, at Chapter 18. Theprotein detection and isolation methods employed herein may also be suchas those described in Harlow and Lane, for example (Harlow, E. and Lane,D., 1988, “Antibodies: A Laboratory Manual”, Cold Spring HarborLaboratory Press, Cold Spring Harbor, N.Y.), which is incorporatedherein by reference in its entirety.

[0188] This can be accomplished, for example, by immunofluorescencetechniques employing a fluorescently labeled antibody (see below)coupled with light microscopic, flow cytometric, or fluorimetricdetection. The antibodies (or fragments thereof) useful in the presentinvention may, additionally, be employed histologically, as inimmunofluorescence or immunoelectron microscopy, for in situ detectionof THBS1 or THBS4 polypeptides. In situ detection may be accomplished byremoving a histological specimen from a subject, and applying thereto alabeled antibody of the present invention. The antibody (or fragment) ispreferably applied by overlaying the labeled antibody (or fragment) ontoa biological sample. Through the use of such a procedure, it is possibleto determine not only the presence of the THBS1 or THBS4 polypeptide,but also its distribution in the examined tissue. Using the presentinvention, one of ordinary skill will readily perceive that any of awide variety of histological methods (such as staining procedures) canbe modified in order to achieve such in situ detection.

[0189] Often a solid phase support or carrier is used as a supportcapable of binding an antigen or an antibody. Well-known supports orcarriers include glass, polystyrene, polypropylene, polyethylene,dextran, nylon, amylases, natural and modified celluloses,polyacrylamides, gabbros, and magnetite. The nature of the carrier canbe either soluble to some extent or insoluble for the purposes of thepresent invention. The support material may have virtually any possiblestructural configuration so long as the coupled molecule is capable ofbinding to an antigen or antibody. Thus, the support configuration maybe spherical, as in a bead, or cylindrical, as in the inside surface ofa test tube, or the external surface of a rod. Alternatively, thesurface may be flat such as a sheet, test strip, etc. Preferred supportsinclude polystyrene beads. Those skilled in the art will know many othersuitable carriers for binding antibody or antigen, or will be able toascertain the same by use of routine experimentation.

[0190] One means for labeling an anti-THBS1 or anti-THBS4 polypeptidespecific antibody is via linkage to an enzyme and use in an enzymeimmunoassay (EIA) (Voller, “The Enzyme Linked Immunosorbent Assay(ELISA)”, Diagnostic Horizons 2:1-7, 1978, Microbiological AssociatesQuarterly Publication, Walkersville, Md.; Voller, et al., J. Clin.Pathol. 31:507-520 (1978); Butler, Meth. Enzymol. 73:482-523 (1981);Maggio, (ed.) Enzyme Immunoassay, CRC Press, Boca Raton, Fla., 1980;Ishikawa, et al., (eds.) Enzyme Immunoassay, Kgaku Shoin, Tokyo, 1981).The enzyme which is bound to the antibody will react with an appropriatesubstrate, preferably a chromogenic substrate, in such a manner as toproduce a chemical moiety which can be detected, for example, byspectrophotometric, fluorimetric or by visual means. Enzymes which canbe used to detectably label the antibody include, but are not limitedto, malate dehydrogenase, staphylococcal nuclease, delta-5-steroidisomerase, yeast alcohol dehydrogenase, alpha-glycerophosphate,dehydrogenase, triose phosphate isomerase, horseradish peroxidase,alkaline phosphatase, asparaginase, glucose oxidase, beta-galactosidase,ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase,glucoamylase and acetylcholinesterase. The detection can be accomplishedby calorimetric methods which employ a chromogenic substrate for theenzyme. Detection may also be accomplished by visual comparison of theextent of enzymatic reaction of a substrate in comparison with similarlyprepared standards.

[0191] Detection may also be accomplished using any of a variety ofother immunoassays. For example, by radioactively labeling theantibodies or antibody fragments, it is possible to detect fingerprintgene wild type or mutant peptides through the use of a radioimmunoassay(RIA) (see, for example, Weintraub, B., Principles of Radioimmunoassays,Seventh Training Course on Radioligand Assay Techniques, The EndocrineSociety, March, 1986, which is incorporated by reference herein). Theradioactive isotope can be detected by such means as the use of a gammacounter or a scintillation counter or by autoradiography.

[0192] It is also possible to label the antibody with a fluorescentcompound. When the fluorescently labeled antibody is exposed to light ofthe proper wave length, its presence can then be detected due tofluorescence. Among the most commonly used fluorescent labelingcompounds are fluorescein isothiocyanate, rhodamine, phycoerythrin,phycocyanin, allophycocyanin, o-phthaldehyde and fluorescamine.

[0193] The antibody can also be detectably labeled using fluorescenceemitting metals such as ¹⁵²Eu, or others of the lanthamide series. Thesemetals can be attached to the antibody using such metal chelating groupsas diethylenetriaminepentacetic acid (DTPA) orethylenediaminetetraacetic acid (EDTA).

[0194] The antibody also can be detectably labeled by coupling it to achemiluminescent compound. The presence of the chemiluminescent-taggedantibody is then determined by detecting the presence of luminescencethat arises during the course of a chemical reaction. Examples ofparticularly useful chemiluminescent labeling compounds are luminol,isoluminol, theromatic acridinium ester, imidazole, acridinium salt andoxalate ester.

[0195] Likewise, a bioluminescent compound may be used to label theantibody of the present invention. Bioluminescence is a type ofchemiluminescence found in biological systems in, which a catalyticprotein increases the efficiency of the chemiluminescent reaction. Thepresence of a bioluminescent protein is determined by detecting thepresence of luminescence. Important bioluminescent compounds forpurposes of labeling are luciferin, luciferase and aequorin.

[0196] If a polymorphic region is located in an exon, either in a codingor non-coding portion of the gene, the identity of the allelic variantcan be determined by determining the molecular structure of the mRNA,pre-mRNA, or cDNA. The molecular structure can be determined using anyof the above described methods for determining the molecular structureof the genomic DNA, e.g., see Example 1.

[0197] The methods described herein may be performed, for example, byutilizing pre-packaged diagnostic kits, such as those described above,comprising at least one probe or primer nucleic acid described herein,which may be conveniently used, e.g., to determine whether a subject hasor is at risk of developing a disease associated with a specific THBS1or THBS4 allelic variant.

[0198] Sample nucleic acid to be analyzed by any of the above-describeddiagnostic and prognostic methods can be obtained from any cell type ortissue of a subject. For example, a subject's bodily fluid (e.g. blood)can be obtained by known techniques (e.g. venipuncture). Alternatively,nucleic acid tests can be performed on dry samples (e.g. hair or skin).Fetal nucleic acid samples can be obtained from maternal blood asdescribed in International Patent Application No. WO91/07660 to Bianchi.Alternatively, amniocytes or chorionic villi may be obtained forperforming prenatal testing.

[0199] Diagnostic procedures may also be performed in situ directly upontissue sections (fixed and/or frozen) of subject tissue obtained frombiopsies or resections, such that no nucleic acid purification isnecessary. Nucleic acid reagents may be used as probes and/or primersfor such in situ procedures (see, for example, Nuovo, G. J., 1992, PCRin situ hybridization: protocols and applications, Raven Press, NY).

[0200] In addition to methods which focus primarily on the detection ofone nucleic acid sequence, profiles may also be assessed in suchdetection schemes. Fingerprint profiles may be generated, for example,by utilizing a differential display procedure, Northern analysis and/orRT-PCR.

[0201] B. Pharmacogenomics

[0202] Knowledge of the identity of the allele of one or more THBS1 orTHBS4 gene polymorphic regions in a subject (the more THBS1 and/or THBS4genetic profile), alone or in conjunction with information of othergenetic defects associated with the same disease (the genetic profile ofthe particular disease) also allows selection and customization of thetherapy, e.g., a particular clinical course of therapy and/or furtherdiagnostic evaluation for a particular disease to the subject's geneticprofile. For example, subjects having a specific allele of a THBS1 orTHBS4 gene, or in combination may or may not exhibit symptoms of aparticular disease or be predisposed to developing symptoms of aparticular disease. Further, if those subjects are symptomatic, they mayor may not respond to a certain drug, e.g., a specific therapeutic usedin the treatment or prevention of a vascular disease or disorder, e.g.,CAD or MI, such as, for example, beta blocker drugs, calcium channelblocker drugs, or nitrate drugs, but may respond to another.Furthermore, they may or may not respond to other treatments, including,for example, use of medical devices for treatment of vascular disease,or surgical and/or non-surgical procedures or courses of treatment.Moreover, if a subject does or does not exhibit symptoms of a particulardisease, the subject may or may not benefit from further diagnosticevaluation, including, for example, use of vascular imaging devices orprocedures. Thus, generation of a THBS1 or THBS4 genetic profile, (e.g.,categorization of alterations in THBS1 or THBS4 genes which areassociated with the development of a particular disease), from apopulation of subjects, who are symptomatic for a disease or conditionthat is caused by or contributed to by a defective and/or deficientTHBS1 or THBS4 gene and/or protein (a THBS1 or THBS4 genetic populationprofile) and comparison of a subject's THBS1 or THBS4 profile to thepopulation profile, permits the selection or design of drugs that areexpected to be safe and efficacious for a particular subject or subjectpopulation (i.e., a group of subjects having the same geneticalteration), as well as the selection or design of a particular clinicalcourse of therapy or further diagnostic evaluations that are expected tobe safe and efficacious for a particular subject or subject population.

[0203] For example, a THBS1 or THBS4 population profile can be performedby determining the THBS1 or THBS4 profile, e.g., the identity of THBS1or THBS4 alleles, in a subject population having a disease, which isassociated with one or more specific alleles of THBS1 or THBS4polymorphic regions. Optionally, the THBS1 or THBS4 population profilecan further include information relating to the response of thepopulation to a THBS1 or THBS4 therapeutic, using any of a variety ofmethods, including, monitoring: 1) the severity of symptoms associatedwith the THBS1 or THBS4 related disease; 2) THBS1 or THBS4 geneexpression level; 3) THBS1 or THBS4 mRNA level; and/or 4) THBS1 or THBS4protein level, and dividing or categorizing the population based onparticular THBS1 or THBS4 alleles. The THBS1 or THBS4 genetic populationprofile can also, optionally, indicate those particular THBS1 or THBS4alleles which are present in subjects that are either responsive ornon-responsive to a particular therapeutic, clinical course of therapy,or diagnostic evaluation. This information or population profile, isthen useful for predicting which individuals should respond toparticular drugs, particular clinical courses of therapy, or diagnosticevaluations based on their individual THBS1 or THBS4 genetic profile.

[0204] In a preferred embodiment, the THBS1 or THBS4 profile is atranscriptional or expression level profile and is comprised ofdetermining the expression level of THBS1 or THBS4 proteins, alone or inconjunction with the expression level of other genes known to contributeto the same disease at various stages of the disease.

[0205] Pharmacogenomic studies can also be performed using transgenicanimals. For example, one can produce transgenic mice, e.g., asdescribed herein, which contain a specific allelic variant of a THBS1 orTHBS4 gene. These mice can be created, e.g., by replacing theirwild-type THBS1 or THBS4 gene with an allele of the human THBS1 or THBS4gene. The response of these mice to specific THBS1 or THBS4 particulartherapeutics, clinical courses of treatment, and/or diagnosticevaluations can then be determined.

[0206] (i) Diagnostic Evaluation

[0207] In one embodiment, the polymorphisms of the present invention areused to determine the most appropriate diagnostic evaluation and todetermine whether or not a subject will benefit from further diagnosticevaluation. For example, if a subject has one or more of the variantalleles at nucleotide positions 55322, 53502, 60793, and/or 58445 of SEQID NO:1, and/or the variant alleles at nucleotide positions 105290,118019, and/or 89835 of SEQ ID NO:3, or the complement thereof, asdescribed herein, that subject is more likely to have or to be at ahigher than normal risk of developing a vascular disease such as CAD orMI. If a subject has one or more of the variant alleles at nucleotidepositions 52861 and/or 49556 of SEQ ID NO:1, or the complement thereof,as described herein, that subject is less likely to have or is at alower than normal risk of developing a vascular disease.

[0208] Thus, in one embodiment, the invention provides methods forclassifying a subject who has, or is at risk for developing, a vasculardisease or disorder as a candidate for further diagnostic evaluation fora vascular disease or disorder comprising the steps of determining theTHBS1 and/or THBS4 genetic profile of the subject, comparing thesubject's THBS1 and/or THBS4 genetic profile to a THBS1 and/or THBS4genetic population profile, and classifying the subject based on theidentified genetic profiles as a subject who is a candidate for furtherdiagnostic evaluation for a vascular disease or disorder

[0209] In a preferred embodiment, the subject's THBS1 genetic profile isdetermined by identifying the nucleotide present at nucleotide positions55322, 53502, 60793, 58445, 52861, and/or 49556 of the referencesequence GI 12583762 of the THBS1 gene. In another preferred embodiment,the subject's THBS4 genetic profile is determined by identifying thenucleotide present at nucleotide positions 105290, 118019, and/or 89835of the reference sequence GI 14916146 of the THBS4 gene.

[0210] Methods of further diagnostic evaluation include use of vascularimaging devices or procedures such as, for example, angiography, cardiacultrasound, coronary angiogram, magnetic resonance imagery, nuclearimaging, CT scan, myocardial perfusion imagery, or electrocardiogram, ormay include genetic analysis, familial health history analysis,lifestyle analysis, exercise stress tests, or any combination thereof.

[0211] In another embodiment, the invention provides methods forselecting an effective vascular imaging device as a diagnostic tool fora vascular disease or disorder comprising the steps of determining theTHBS1 and/or THBS4 genetic profile of the subject; comparing thesubject's THBS1 and/or THBS4 genetic profile to a THBS1 and/or THBS4genetic population profile; and selecting an effective vascular imagingdevice or procedure as a diagnostic tool for a vascular disease ordisorder. In a preferred embodiment, the vascular imaging device isselected from the group consisting of angiography, cardiac ultrasound,coronary angiogram, magnetic resonance imagery, nuclear imaging, CTscan, myocardial perfusion imagery, electrocardiogram, or anycombination thereof

[0212] (ii) Clinical Course of Therapy

[0213] In another aspect, the polymorphisms of the present invention areused to determine the most appropriate clinical course of therapy for asubject who has or is at risk of a vascular disease or disorder, andwill aid in the determination of whether the subject will benefit fromsuch clinical course of therapy, as determined by identification of one,or preferably, both of the polymorphisms of the invention. If a subjecthas one or more of the variant alleles at nucleotide positions 55322,53502, 60793, and/or 58445 of SEQ ID NO:1 and/or the variant alleles atnucleotide positions 105290, 118019, and/or 89835 of SEQ ID NO:3, or thecomplement thereof, that subject is more likely to have or to be at ahigher than normal risk of developing a vascular disease such as CAD orMI. If a subject has one or more of the variant alleles at nucleotidepositions 52861 and/or 49556 of SEQ ID NO:1, or the complement thereof,as described herein, that subject is less likely to have or to be atlower than normal risk of developing a vascular disease

[0214] Thus, in one aspect, the invention relates to the SNPs identifiedas described herein, both singly and in combination, as well as to theuse of these SNPs, and others in these genes, particularly those nearbyin linkage disequilibrium with these SNPs, both singly and incombination, for prediction of a particular clinical course of therapyfor a subject who has, or is at risk for developing, a vascular disease.In one embodiment, the invention provides a method for determiningwhether a subject will benefit from a particular course of therapy bydetermining the presence of one, or preferably both, of thepolymorphisms of the invention. For example, the determination of thepolymorphisms of the invention, singly, or in combination, will aid inthe determination of whether an individual will benefit from surgicalrevascularization and/or will benefit by the implantation of a stentfollowing surgical revascularization, and will aid in the determinationof the likelihood of success or failure of a particular clinical courseof therapy.

[0215] In one embodiment, the invention provides methods for classifyinga subject who has, or is at risk for developing, a vascular disease ordisorder as a candidate for a particular clinical course of therapy fora vascular disease or disorder comprising the steps of determining theTHBS1 and/or THBS4 genetic profile of the subject; comparing thesubject's THBS1 and/or THBS4 genetic profile to a THBS1 and/or THBS4genetic population profile; and classifying the subject based on theidentified genetic profiles as a subject who is a candidate for aparticular clinical course of therapy for a vascular disease ordisorder.

[0216] In another embodiment, the invention provides methods forselecting an effective clinical course of therapy to treat a subject whohas, or is at risk for developing, a vascular disease or disordercomprising the steps of: determining the THBS1 and/or THBS4 geneticprofile of the subject; comparing the subject's THBS1 and/or THBS4genetic profile to a THBS1 and/or THBS4 genetic population profile; andselecting an appropriate clinical course of therapy for treatment of asubject who has, or is at risk for developing, a vascular disease ordisorder.

[0217] An appropriate clinical course of therapy may include, forexample, a lifestyle change, including, for example, a change in diet orenvironment. Other clinical courses of therapy include, but are notlimited to, use of surgical procedures or medical devices. Surgicalprocedures for the treatment of vascular disorders, includes, forexample, surgical revascularization, such as angioplasty, e.g.,percutaneous transluminal coronary balloon angioplasty (PTCA), or laserangioplasty, or coronary bypass grafting (CABG). Medical devices used inthe treatment or prevention of vascular diseases or disorders, include,for example, devices used in angioplasty, such as balloon angioplasty orlaser angioplasty, a device used in coronary revascularization, or astent, a defibrillator, a pacemaker, or any combination thereof. Medicaldevices may also be used in combination with modulators of THBS1 and/orTHBS4 gene expression or THBS1 and/or THBS4 protein activity.

[0218] C. Monitoring Effects of THBS1 or THBS4 Therapeutics DuringClinical Trials

[0219] The present invention provides a method for monitoring theeffectiveness of treatment of a subject with a THBS1 or THBS4therapeutic e.g., a modulator or agent (e.g., an agonist, antagonist,such as, for example, a peptidomimetic, protein, peptide, nucleic acid,ribozyme, small molecule, or other drug candidate identified, e.g., bythe screening assays described herein) comprising the steps of (i)obtaining a preadministration sample from a subject prior toadministration of the agent; (ii) detecting the level of expression oractivity of a THBS1 or THBS4 protein, mRNA or gene in thepreadministration sample; (iii) obtaining one or morepost-administration samples from the subject; (iv) detecting the levelof expression or activity of the THBS1 or THBS4 protein, mRNA or gene inthe post-administration samples; (v) comparing the level of expressionor activity of the THBS1 or THBS4 protein, mRNA, or gene in thepreadministration sample with those of the THBS1 or THBS4 protein, mRNA,or gene in the post administration sample or samples; and (vi) alteringthe administration of the agent to the subject accordingly. For example,increased administration of the agent may be desirable to increase theexpression or activity of THBS1 or THBS4 to higher levels than detected,i.e., to increase the effectiveness of the agent. Alternatively,decreased administration of the agent may be desirable to decreaseexpression or activity of THBS1 or THBS4 to lower levels than detected,i.e., to decrease the effectiveness of the agent.

[0220] Cells of a subject may also be obtained before and afteradministration of a THBS1or THBS4 therapeutic to detect the level ofexpression of genes other than THBS1 or THBS4, to verify that the THBS1or THBS4 therapeutic does not increase or decrease the expression ofgenes which could be deleterious. This can be done, e.g., by using themethod of transcriptional profiling. Thus, mRNA from cells exposed invivo to a THBS1 or THBS4 therapeutic and mRNA from the same type ofcells that were not exposed to the THBS1 or THBS4 therapeutic could bereverse transcribed and hybridized to a chip containing DNA fromnumerous genes, to thereby compare the expression of genes in cellstreated and not treated with a THBS1 or THBS4 therapeutic. If, forexample a THBS1 or THBS4 therapeutic turns on the expression of aproto-oncogene in a subject, use of this particular THBS1 or THBS4therapeutic may be undesirable.

[0221] D. Methods of Treatment

[0222] The present invention provides for both prophylactic andtherapeutic methods of treating a subject having or likely to develop adisorder associated with specific THBS1 or THBS4 alleles and/or aberrantTHBS1 or THBS4 expression or activity, e.g., vascular diseases ordisorders.

[0223] i) Prophylactic Methods

[0224] In one aspect, the invention provides a method for preventing adisease or disorder associated with a specific THBS1 or THBS4 allelesuch as a vascular disease or disorder, e.g., CAD or MI, and medicalconditions resulting therefrom, by administering to the subject an agentwhich counteracts the unfavorable biological effect of the specificTHBS1 or THBS4 allele. Subjects at risk for such a disease can beidentified by a diagnostic or prognostic assay, e.g., as describedherein. Administration of a prophylactic agent can occur prior to themanifestation of symptoms associated with specific THBS1 or THBS4alleles, such that a disease or disorder is prevented or, alternatively,delayed in its progression. Depending on the identity of the THBS1 orTHBS4 allele in a subject, a compound that counteracts the effect ofthis allele is administered. The compound can be a compound modulatingthe activity of THBS1 or THBS4, e.g., a THBS1 or THBS4 inhibitor. Thetreatment can also be a specific lifestyle change, e.g., a change indiet or an environmental alteration. In particular, the treatment can beundertaken prophylactically, before any other symptoms are present. Sucha prophylactic treatment could thus prevent the development of aberrantvascular activity, e.g., the production of atherosclerotic plaqueleading to, e.g., CAD or MI. The prophylactic methods are similar totherapeutic methods of the present invention and are further discussedin the following subsections.

[0225] (ii) Therapeutic Methods

[0226] The invention further provides methods of treating a subjecthaving a disease or disorder associated with a specific allelic variantof a polymorphic region of a THBS1 or THBS4 gene. Preferred diseases ordisorders include vascular diseases and disorders, and disordersresulting therefrom (e.g., such as, for example, atherosclerosis, CAD,MI, ischemia, stroke, peripheral vascular diseases, venousthromboembolism and pulmonary embolism).

[0227] In one embodiment, the method comprises (a) determining theidentity of an allelic variant of a THBS1 and/or THBS4 gene, orpreferably, the identities of one or more of nucleotide residue 55322,53502, 60793, 58445, 52861, and/or 49556 of SEQ ID NO:1 (the THBS1gene), and/or residues 105290, 118019, and/or 89835 of SEQ ID NO:3 (theTHBS4 gene), or the complements thereof; and (b) administering to thesubject a compound that compensates for the effect of the specificallelic variant(s). The polymorphic region can be localized at anylocation of the gene, e.g., in a regulatory element (e.g., in a 5′upstream regulatory element), in an exon, (e.g., coding region of anexon), in an intron, or at an exon/intron border. Thus, depending on thesite of the polymorphism in the THBS1 or THBS4 gene, a subject having aspecific variant of the polymorphic region which is associated with aspecific disease or condition, can be treated with compounds whichspecifically compensate for the effect of the allelic variant.

[0228] In a preferred embodiment, the identity of one or more of thenucleotides present at the nucleotide residue 55322, 53502, 60793,58445, 52861, and/or 49556 of SEQ ID NO:1 (the THBS1 gene), and/orresidues 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof.

[0229] If a subject has one or more of the variant alleles at nucleotide55322, 53502, 60793, and/or 58445 of SEQ ID NO:1 (the THBS1 gene),and/or residues 105290, 118019, and/or 89835 of SEQ ID NO:3, or thecomplement thereof, that subject is at a higher than normal risk ofdeveloping a vascular disease such as CAD or MI. If a subject has one ormore of the variant alleles at nucleotide positions 52861 and/or 49556of SEQ ID NO:1, or the complement thereof, as described herein, thatsubject is less likely to have or to be at lower than normal risk ofdeveloping a vascular disease.

[0230] A mutation can be a substitution, deletion, and/or addition of atleast one nucleotide relative to the wild-type allele (i.e., thereference sequence). Depending on where the mutation is located in theTHBS1 or THBS4 gene, the subject can be treated to specificallycompensate for the mutation. For example, if the mutation is present inthe coding region of the gene and results in a more active THBS1 orTHBS4 protein, the subject can be treated, e.g., by administration tothe subject of a modulator, e.g., a therapeutic or course of clinicaltreatment which treat, prevents, or ameliorates a vascular disease ordisorder. Normal THBS1 and/or THBS4 protein can also be used tocounteract or compensate for the endogenous mutated form of the THBS1and/or THBS4 protein. Normal THBS1 and/or THBS4 protein can be directlydelivered to the subject or indirectly by gene therapy wherein somecells in the subject are transformed or transfected with an expressionconstruct encoding wild-type THBS1 and/or THBS4 protein. Nucleic acidsencoding reference human THBS1 and/or THBS4 protein are set forth in SEQID NOs.:1 and 3, respectively.

[0231] Yet in another embodiment, the invention provides methods fortreating a subject having a mutated THBS1 and/or THBS4 gene, in whichthe mutation is located in a regulatory region of the gene. Such aregulatory region can be localized in the 5′ upstream regulatory elementof the gene, in the 5′ or 3′ untranslated region of an exon, or in anintron. A mutation in a regulatory region can result in increasedproduction of THBS1 and/or THBS4 protein, decreased production of THBS1and/or THBS4 protein, or production of THBS1 and/or THBS4 having anaberrant tissue distribution. The effect of a mutation in a regulatoryregion upon the THBS1 and/or THBS4 protein can be determined, e.g., bymeasuring the THBS1 and/or THBS4 protein level or mRNA level in cellshaving a THBS1and/or THBS4 gene having this mutation and which, normally(i.e., in the absence of the mutation) produce THBS1 and/or THBS4protein. The effect of a mutation can also be determined in vitro. Forexample, if the mutation is in the 5′ upstream regulatory element, areporter construct can be constructed which comprises the mutated 5′upstream regulatory element linked to a reporter gene, the constructtransfected into cells, and comparison of the level of expression of thereporter gene under the control of the mutated 5′ upstream regulatoryelement and under the control of a wild-type 5′ upstream regulatoryelement. Such experiments can also be carried out in mice transgenic forthe mutated 5′ upstream regulatory element. If the mutation is locatedin an intron, the effect of the mutation can be determined, e.g., byproducing transgenic animals in which the mutated THBS1 and/or THBS4gene has been introduced and in which the wild-type gene may have beenknocked out. Comparison of the level of expression of THBS1 and/or THBS4in the mice transgenic for the mutant human THBS1 and/or THBS4 gene withmice transgenic for a wild-type human THBS1 and/or THBS4 gene willreveal whether the mutation results in increased, or decreased synthesisof the THBS1 and/or THBS4 protein and/or aberrant tissue distribution ofTHBS1 and/or THBS4 protein. Such analysis could also be performed incultured cells, in which the human mutant THBS1 and/or THBS4 gene isintroduced and, e.g., replaces the endogenous wild-type THBS1 and/orTHBS4 gene in the cell. Thus, depending on the effect of the mutation ina regulatory region of a THBS1 and/or THBS4 gene, a specific treatmentcan be administered to a subject having such a mutation. Accordingly, ifthe mutation results in increased THBS1 and/or THBS4 protein levels, thesubject can be treated by administration of a compound which reducesTHBS1 and/or THBS4 protein production, e.g., by reducing THBS1 and/orTHBS4 gene expression or a compound which inhibits or reduces theactivity of THBS1 and/or THBS4.

[0232] A correlation between drug responses and specific alleles ofTHBS1 and/or THBS4 can be shown, for example, by clinical studieswherein the response to specific drugs of subjects having differentallelic variants of a polymorphic region of a THBS1 and/or THBS4 gene iscompared. Such studies can also be performed using animal models, suchas mice having various alleles of human THBS1 and/or THBS4 genes and inwhich, e.g., the endogenous THBS1 and/or THBS4 has been inactivated suchas by a knock-out mutation. Test drugs are then administered to the micehaving different human THBS1 and/or THBS4 alleles and the response ofthe different mice to a specific compound is compared. Accordingly, theinvention provides assays for identifying the drug which will be bestsuited for treating a specific disease or condition in a subject. Forexample, it will be possible to select drugs which will be devoid oftoxicity, or have the lowest level of toxicity possible for treating asubject having a disease or condition.

[0233] Other Uses For the Nucleic Acid Molecules of the Invention

[0234] The identification of different alleles of THBS1 or THBS4 canalso be useful for identifying an individual among other individualsfrom the same species. For example, DNA sequences can be used as afingerprint for detection of different individuals within the samespecies (Thompson, J. S. and Thompson, eds., Genetics in Medicine, W BSaunders Co., Philadelphia, Pa. (1991)). This is useful, for example, inforensic studies and paternity testing, as described below.

[0235] A. Forensics

[0236] Determination of which specific allele occupies a set of one ormore polymorphic sites in an individual identifies a set of polymorphicforms that distinguish the individual from others in the population. Seegenerally National Research Council, The Evaluation of Forensic DNAEvidence (Eds. Pollard et al., National Academy Press, DC, 1996). Themore polymorphic sites that are analyzed, the lower the probability thatthe set of polymorphic forms in one individual is the same as that in anunrelated individual. Preferably, if multiple sites are analyzed, thesites are unlinked. Thus, the polymorphisms of the invention can be usedin conjunction with known polymorphisms in distal genes. Preferredpolymorphisms for use in forensics are biallelic because the populationfrequencies of two polymorphic forms can usually be determined withgreater accuracy than those of multiple polymorphic forms atmulti-allelic loci.

[0237] The capacity to identify a distinguishing or unique set ofpolymorphic markers in an individual is useful for forensic analysis.For example, one can determine whether a blood sample from a suspectmatches a blood or other tissue sample from a crime scene by determiningwhether the set of polymorphic forms occupying selected polymorphicsites is the same in the suspect and the sample. If the set ofpolymorphic markers does not match between a suspect and a sample, itcan be concluded (barring experimental error) that the suspect was notthe source of the sample. If the set of markers is the same in thesample as in the suspect, one can conclude that the DNA from the suspectis consistent with that found at the crime scene. If frequencies of thepolymorphic forms at the loci tested have been determined (e.g., byanalysis of a suitable population of individuals), one can perform astatistical analysis to determine the probability that a match ofsuspect and crime scene sample would occur by chance.

[0238] p(ID) is the probability that two random individuals have thesame polymorphic or allelic form at a given polymorphic site. Forexample, in biallelic loci, four genotypes are possible: AA, AB, BA, andBB. If alleles A and B occur in a haploid genome of the organism withfrequencies x and y, the probability of each genotype in a diploidorganism is (see WO 95/12607):

[0239] Homozygote: p(AA)=x²

[0240] Homozygote: p(BB)=y²=(1−x)²

[0241] Single Heterozygote: p(AB)=p(BA)=xy=x(1−x)

[0242] Both Heterozygotes: p(AB+BA)=2xy=2×(1−x) The probability ofidentity at one locus (i.e., the probability that two individuals,picked at random from a population will have identical polymorphic formsat a given locus) is given by the equation: p(ID)=(x²).

[0243] These calculations can be extended for any number of polymorphicforms at a given locus. For example, the probability of identity p(ID)for a 3-allele system where the alleles have the frequencies in thepopulation of x, y, and z, respectively, is equal to the sum of thesquares of the genotype frequencies:P(ID)=x⁴+(2xy)²+(2yz)²+(2xz)²+z⁴+y⁴.

[0244] In a locus of n alleles, the appropriate binomial expansion isused to calculate p(ID) and p(exc).

[0245] The cumulative probability of identity (cum p(ID)) for each ofmultiple unlinked loci is determined by multiplying the probabilitiesprovided by each locus: cum p(ID)=p(ID1)p(ID2)p(ID3) . . . p(IDn).

[0246] The cumulative probability of non-identity for n loci (i.e., theprobability that two random individuals will be difference at 1 or moreloci) is given by the equation: cum p(nonID)=1-cum p(ID).

[0247] If several polymorphic loci are tested, the cumulativeprobability of non-identity for random individuals becomes very high(e.g., one billion to one). Such probabilities can be taken into accounttogether with other evidence in determining the guilt or innocence ofthe suspect.

[0248] B. Paternity Testing

[0249] The object of paternity testing is usually to determine whether amale is the father of a child. In most cases, the mother of the child isknown, and thus, it is possible to trace the mother's contribution tothe child's genotype. Paternity testing investigates whether the part ofthe child's genotype not attributable to the mother is consistent tothat of the putative father. Paternity testing can be performed byanalyzing sets of polymorphisms in the putative father and in the child.

[0250] If the set of polymorphisms in the child attributable to thefather does not match the set of polymorphisms of the putative father,it can be concluded, barring experimental error, that that putativefather is not the real father. If the set of polymorphisms in the childattributable to the father does match the set of polymorphisms of theputative father, a statistical calculation can be performed to determinethe probability of a coincidental match.

[0251] The probability of parentage exclusion (representing theprobability that a random male will have a polymorphic form at a givenpolymorphic site that makes him incompatible as the father) is given bythe equation (see WO 95/12607): p(exc)=xy(1−xy), where x and y are thepopulation frequencies of alleles A and B of a biallelic polymorphicsite.

[0252] (At a triallelic sitep(exc)=xy(1−xy)+yz(1−yz)+xz(1−xz)+3xyz(1−xyz)), where x, y, and z andthe respective populations frequencies of alleles A, B, and C).

[0253] The probability of non-exclusion is: p(non-exc)=1−p(exc).

[0254] The cumulative probability of non-exclusion (representing thevalues obtained when n loci are is used) is thus:

[0255] Cum p(non-exc)=p(non-exc1)p(non-exc2)p(non-exc3) . . .p(non-excn).

[0256] The cumulative probability of the exclusion for n loci(representing the probability that a random male will be excluded: cump(exc)=1−cum p(non-exc).

[0257] If several polymorphic loci are included in the analysis, thecumulative probability of exclusion of a random male is very high. Thisprobability can be taken into account in assessing the liability of aputative father whose polymorphic marker set matches the child'spolymorphic marker set attributable to his or her father.

[0258] C. Kits

[0259] As set forth herein, the invention provides methods, e.g.,diagnostic and therapeutic methods, e.g., for determining the type ofallelic variant of a polymorphic region present in a THBS1 or THBS4gene, such as a human THBS1 or THBS4 gene. In preferred embodiments, themethods use probes or primers comprising nucleotide sequences which arecomplementary to a polymorphic region of a THBS1 or THBS4 gene (SEQ IDNOs: 5, 6, 7, 8, 9,10, 11, 12, or 13). In a preferred embodiment, themethods use probes or primers comprising nucleotide sequences which arecomplementary to a polymorphic region of a thrombospondin gene, e.g., aTHBS1 and THBS4 gene. Accordingly, the invention provides kits forperforming these methods. In a preferred embodiment, the kit comprisesprobes or primers comprising nucleotide sequences which arecomplementary to the one or more of the variant alleles or referencealleles at nucleotide positions 55322, 53502, 60793, 58445, 52861,and/or 49556 of SEQ ID NO:1 (the THBS1 gene), and/or residues 105290,118019, and/or 89835 of SEQ ID NO:3, or the complement thereof. In apreferred embodiment, the invention provides a kit for determiningwhether a subject has or is at risk of developing a disease or conditionassociated with a specific allelic variant of a THBS1 or THBS4polymorphic region. In an even more preferred embodiment, the disease ordisorder is characterized by an abnormal THBS1 or THBS4 activity. In aneven more preferred embodiment, the invention provides a kit fordetermining whether a subject has or is or is not at risk of developinga vascular disease, e.g., atherosclerosis, CAD, MI, ischemia, stroke,peripheral vascular diseases, venous thromboembolism and pulmonaryembolism.

[0260] A preferred kit provides reagents for determining whether asubject is likely to develop a vascular disease, e.g., CAD or MI.

[0261] Preferred kits comprise at least one probe or primer which iscapable of specifically hybridizing under stringent conditions to aTHBS1 or THBS4 sequence or polymorphic region and instructions for use.The kits preferably comprise at least one of the above described nucleicacids. Preferred kits for amplifying at least a portion of a THBS1and/or THBS4 gene comprise at least two primers, at least one of whichis capable of hybridizing to an allelic variant sequence.

[0262] The kits of the invention can also comprise one or more controlnucleic acids or reference nucleic acids, such as nucleic acidscomprising a THBS1 or THBS4 intronic sequence. For example, a kit cancomprise primers for amplifying a polymorphic region of a THBS1 or THBS4gene and a control DNA corresponding to such an amplified DNA and havingthe nucleotide sequence of a specific allelic variant. Thus, directcomparison can be performed between the DNA amplified from a subject andthe DNA having the nucleotide sequence of a specific allelic variant. Inone embodiment, the control nucleic acid comprises at least a portion ofa THBS1 or THBS4 gene of an individual who does not have a vasculardisease, or a disease or disorder associated with an aberrant THBS1 orTHBS4 activity. Yet other kits of the invention comprise at least onereagent necessary to perform the assay. For example, the kit cancomprise an enzyme. Alternatively the kit can comprise a buffer or anyother necessary reagent.

[0263] D. Electronic Apparatus Readable Media and Arrays

[0264] Electronic apparatus readable media comprising a polymorphism ofthe present invention is also provided. As used herein, “electronicapparatus readable media” and “computer readable media,” which are usedinterchangeably herein, refers to any suitable medium for storing,holding or containing data or information that can be read and accesseddirectly by an electronic apparatus. Such media can include, but are notlimited to: magnetic storage media, such as floppy discs, hard discstorage medium, and magnetic tape; optical storage media such as compactdisc; electronic storage media such as RAM, ROM, EPROM, EEPROM and thelike; general hard disks and hybrids of these categories such asmagnetic/optical storage media. The medium is adapted or configured forhaving recorded thereon a marker of the present invention.

[0265] As used herein, the term “electronic apparatus” is intended toinclude any suitable computing or processing apparatus or other deviceconfigured or adapted for storing data or information. Examples ofelectronic apparatus suitable for use with the present invention includestand-alone computing apparatus; networks, including a local areanetwork (LAN), a wide area network (WAN) Internet, Intranet, andExtranet; electronic appliances such as a personal digital assistants(PDAs), cellular phone, pager and the like; and local and distributedprocessing systems.

[0266] As used herein, “recorded” refers to a process for storing orencoding information on the electronic apparatus readable medium. Thoseskilled in the art can readily adopt any of the presently known methodsfor recording information on known media to generate manufacturescomprising the polymorphisms of the present invention.

[0267] A variety of software programs and formats can be used to storethe polymorphism information of the present invention on the electronicapparatus readable medium. For example, the polymorphic sequence can berepresented in a word processing text file, formatted incommercially-available software such as WordPerfect and MicroSoft Word,or represented in the form of an ASCII file, stored in a databaseapplication, such as DB2, Sybase, Oracle, or the like, as well as inother forms. Any number of data processor structuring formats (e.g.,text file or database) may be employed in order to obtain or create amedium having recorded thereon the markers of the present invention.

[0268] By providing the polymorphisms of the invention in readable form,singly or in combination, one can routinely access the polymorphisminformation for a variety of purposes. For example, one skilled in theart can use the sequences of the polymorphisms of the present inventionin readable form to compare a target sequence or target structural motifwith the sequence information stored within the data storage means.Search means are used to identify fragments or regions of the sequencesof the invention which match a particular target sequence or targetmotif.

[0269] The present invention therefore provides a medium for holdinginstructions for performing a method for determining whether a subjecthas a vascular disease or a pre-disposition to a vascular disease,wherein the method comprises the steps of determining the presence orabsence of a polymorphism and based on the presence or absence of thepolymorphism, determining whether the subject has a vascular disease ora pre-disposition to a vascular disease and/or recommending a particularclinical course of therapy or diagnostic evaluation for the vasculardisease or pre-vascular disease condition.

[0270] The present invention further provides in an electronic systemcomprising a processor and/or in a network, a method for determiningwhether or not a subject has a vascular disease or a pre-disposition tovascular disease associated with a polymorphism as described hereinwherein the method comprises the steps of determining the presence orabsence of the polymorphism, and based on the presence or absence of thepolymorphism, determining whether the subject has a vascular disease ora pre-disposition to a vascular disease, and/or recommending aparticular treatment for the vascular disease or pre-vascular diseasecondition. In one embodiment, the processor implements the functionalityof obtaining information from the subject indicative of the presence orabsence of the polymorphic region. In another embodiment, the processorfurther implements the functionality of receiving phenotypic informationassociated with the subject. In yet another embodiment, the processorfurther implements the functionality of acquiring from a networkphenotypic information associated with the subject. The method mayfurther comprise the step of receiving phenotypic information associatedwith the subject and/or acquiring from a network phenotypic informationassociated with the subject.

[0271] The present invention also provides in a network system, a methodfor determining whether a subject has vascular disease or apre-disposition to vascular disease associated with a polymorphism, saidmethod comprising the steps of receiving information associated with thepolymorphism, receiving phenotypic information associated with thesubject, acquiring information from the network corresponding to thepolymorphism and/or vascular disease, and based on one or more of thephenotypic information, the polymorphism, and the acquired information,determining whether the subject has a vascular disease or apre-disposition to a vascular disease. The method may further comprisethe step of recommending a particular treatment for the vascular diseaseor pre-vascular disease condition. In one embodiment, the network systemcomprises a server and a work station operatively connected to theserver via the network.

[0272] The present invention also provides a method for determiningwhether a subject has a vascular disease or a pre-disposition to avascular disease, said method comprising the steps of receivinginformation associated with the polymorphism, receiving phenotypicinformation associated with the subject, acquiring information from thenetwork corresponding to the polymorphism and/or vascular disease, andbased on one or more of the phenotypic information, the polymorphism,and the acquired information, determining whether the subject hasvascular disease or a pre-disposition to vascular disease. The methodmay further comprise the step of recommending a particular treatment forthe vascular disease or pre-vascular disease condition.

[0273] E. Personalized Health Assessment

[0274] Methods and systems of assessing personal health and risk fordisease, e.g., vascular disease, in a subject, using the polymorphismsand associations of the instant invention are also provided. The methodsprovide personalized health care knowledge to individuals as well as totheir health care providers, as well as to health care companies. Itwill be appreciated that the term “health care providers” is not limitedto physicians but can be any source of health care. The methods andsystems provide personalized information including a personal healthassessment report that can include a personalized molecular profile,e.g., a THBS1 and/or THBS4 genetic profile, a health profile, or both.Overall, the methods and systems as described herein providepersonalized information for individuals and patient management toolsfor healthcare providers and/or subjects using a variety ofcommunications networks such as, for example, the Internet. U.S. PatentApplication Serial No. 60/266,082, filed Feb. 1, 2001, entitled “Methodsand Systems for Personalized Health Assessment,” further describespersonalized health assessment methods, systems, and apparatus, and isexpressly incorporated herein by reference.

[0275] In one aspect, the invention provides an Internet-based methodfor assessing a subject's risk for vascular disease, e.g., CAD or MI. Inone embodiment, the method comprises obtaining a biological sample froma subject, analyzing the biological sample to determine the presence orabsence of a polymorphic region of THBS1 and/or THBS4, and providingresults of the analysis to the subject via the Internet, wherein thepresence of a polymorphic region of THBS1 and/or THBS4 indicates anincreased or decreased risk for vascular disease. In another embodiment,the method comprises analyzing data from a biological sample from asubject relating to the presence or absence of a polymorphic region ofTHBS1 and/or THBS4 and providing results of the analysis to the subjectvia the Internet, wherein the presence of a polymorphic region of THBS1and/or THBS4 indicates an increased or decreased risk for vasculardisease.

[0276] It will be appreciated that the phrase “wherein the presence of apolymorphic region of THBS1 and/or THBS4 indicates an increased ordecreased risk for vascular disease” includes an increased or higherthan normal risk of developing a vascular disease indicated by a subjecthaving one or more of the variant alleles at nucleotide residues 55322,53502, 60793, and/or 58445 of SEQ ID NO:1 (the THBS1 gene), and/orresidues 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof. The phrase also includes a decreased or lower than normal riskof developing a vascular disease indicated by a subject having one ormore of the variant alleles at nucleotide positions 52861 and/or 49556of SEQ ID NO:1, or the complement thereof.

[0277] The terms “Internet” and/or “communications network” as usedherein refer to any suitable communication link, which permitselectronic communications. It should be understood that these terms arenot limited to “the Internet” or any other particular system or type ofcommunication link. That is, the terms “Internet” and/or “communicationsnetwork” refer to any suitable communication system, includingextra-computer system and intra-computer system communications. Examplesof such communication systems include internal busses, local areanetworks, wide area networks, point-to-point shared and dedicatedcommunications, infra-red links, microwave links, telephone links, CATVlinks, satellite and radio links, and fiber-optic links. The terms“Internet” and/or “communications network” can also refer to anysuitable communications system for sending messages between remotelocations, directly or via a third party communication provider such asAT&T. In this instance, messages can be communicated via telephone orfacsimile or computer synthesized voice telephone messages with orwithout voice or tone recognition, or any other suitable communicationstechnique.

[0278] In another aspect, the methods of the invention also providemethods of assessing a subject's risk for vascular disease, e.g., CAD orMI. In one embodiment, the method comprises obtaining information fromthe subject regarding the polymorphic region of an F7 gene, throughe.g., obtaining a biological sample from the individual, analyzing thesample to obtain the subject's THBS1 and/or THBS4 genetic profile,representing the THBS1 and/or THBS4 genetic profile information asdigital genetic profile data, electronically processing the THBS1 and/orTHBS4 digital genetic profile data to generate a risk assessment reportfor vascular disease, and displaying the risk assessment report on anoutput device, where the presence of a polymorphic region of THBS1and/or THBS4 indicates an increased or decreased risk for vasculardisease. In another embodiment, the method comprises analyzing asubject's THBS1 and/or THBS4 genetic profile, representing the THBS1and/or THBS4 genetic profile information as digital genetic profiledata, electronically processing the THBS1 and/or THBS4 digital geneticprofile data to generate a risk assessment report for vascular disease,and displaying the risk assessment report on an output device, where thepresence of a polymorphic region of THBS1 and/or THBS4 indicates anincreased or decreased risk for vascular disease, e.g., CAD or MI.Additional health information may be provided and can be utilized togenerate the risk assessment report. Such information includes, but isnot limited to, information regarding one or more of age, sex, ethnicorigin, diet, sibling health, parental health, clinical symptoms,personal health history, blood test data, weight, and alcohol use, druguse, nicotine use, and blood pressure.

[0279] The THBS1 and/or THBS4 digital genetic profile data may betransmitted via a communications network, e.g., the Internet, to amedical information system for processing.

[0280] In yet another aspect the invention provides a medicalinformation system for assessing a subject's risk for vascular diseasecomprising a means for obtaining information from the subject regardingthe polymorphic region of an F7 gene, through e.g., obtaining abiological sample from the individual to obtain a THBS1 and/or THBS4genetic profile, a means for representing the THBS1 and/or THBS4 geneticprofile as digital molecular data, a means for electronically processingthe THBS1 and/or THBS4 digital genetic profile to generate a riskassessment report for vascular disease, and a means for displaying therisk assessment report on an output device, where the presence of apolymorphic region of THBS1 and/or THBS4 indicates an increased ordecreased risk for vascular disease.

[0281] In another aspect, the invention provides a computerized methodof providing medical advice to a subject comprising obtaininginformation from the subject regarding the polymorphic region of an F7gene, through e.g., obtaining a biological sample from the subject,analyzing the subject's biological sample to determine the subject'sTHBS1 and/or THBS4 genetic profile, and, based on the subject's THBS1and/or THBS4 genetic profile, determining the subject's risk forvascular disease. Medical advice may be then provided electronically tothe subject, based on the subject's risk for vascular disease. Themedical advice may comprise, for example, recommending one or more ofthe group consisting of: further diagnostic evaluation, use of medicalor surgical devices, administration of medication, or lifestyle change.Additional health information may also be obtained from the subject andmay also be used to provide the medical advice.

[0282] In another aspect, the invention includes a method forself-assessing risk for a vascular disease. The method comprisesproviding information from the subject regarding the polymorphic regionof an F7 gene, through e.g., providing a biological sample for geneticanalysis, and accessing an electronic output device displaying resultsof the genetic analysis, thereby self-assessing risk for a vasculardisease, where the presence of a polymorphic region of THBS1 and/orTHBS4 indicates an increased or decreased risk for vascular disease.

[0283] In another aspect, the invention provides a method ofself-assessing risk for vascular disease comprising providinginformation from the subject regarding the polymorphic region of an F7gene, through e.g., providing a biological sample, accessing THBS1and/or THBS4 digital genetic profile data obtained from the biologicalsample, the THBS1 and/or THBS4 digital genetic profile data beingdisplayed via an output device, where the presence of a polymorphicregion of THBS1 and/or THBS4 indicates an increased or decreased riskfor vascular disease.

[0284] An output device may be, for example, a CRT, printer, or website.An electronic output device may be accessed via the Internet.

[0285] The biological sample may be obtained from the individual at alaboratory company. In one embodiment, the laboratory company processesthe biological sample to obtain THBS1 and/or THBS4 genetic profile data,represents at least some of the THBS1 and/or THBS4 genetic profile dataas digital genetic profile data, and transmits the THBS1 and/or THBS4digital genetic profile data via a communications network to a medicalinformation system for processing. The biological sample may also beobtained from the subject at a draw station. A draw station processesthe biological sample to obtain THBS1 and/or THBS4 genetic profile dataand transfers the data to a laboratory company. The laboratory companythen represents at least some of the THBS1 and/or THBS4 genetic profiledata as digital genetic profile data, and transmits the THBS1 and/orTHBS4 digital genetic profile data via a communications network to amedical information system for processing.

[0286] In another aspect, the invention provides a method for a healthcare provider to generate a personal health assessment report for anindividual. The method comprises counseling the individual to provide abiological sample and authorizing a draw station to take a biologicalsample from the individual and transmit molecular information from thesample to a laboratory company, where the molecular informationcomprises the presence or absence of a polymorphic region of THBS1and/or THBS4. The health care provider then requests the laboratorycompany to provide digital molecular data corresponding to the molecularinformation to a medical information system to electronically processthe digital molecular data and digital health data obtained from theindividual to generate a health assessment report, receives the healthassessment report from the medical information system, and provides thehealth assessment report to the individual.

[0287] In still another aspect, the invention provides a method ofassessing the health of an individual. The method comprises obtaininghealth information from the individual using an input device (e.g., akeyboard, touch screen, hand-held device, telephone, wireless inputdevice, or interactive page on a website), representing at least some ofthe health information as digital health data, obtaining a biologicalsample from the individual, and processing the biological sample toobtain molecular information, where the molecular information comprisesthe presence or absence of a polymorphic region of THBS1 and/or THBS4.At least some of the molecular information and health data is thenpresented as digital molecular data and electronically processed togenerate a health assessment report. The health assessment report isthen displayed on an output device. The health assessment report cancomprise a digital health profile of the individual. The molecular datacan comprise protein sequence data, and the molecular profile cancomprise a proteomic profile. The molecular data can also compriseinformation regarding one or more of the absence, presence, or level, ofone or more specific proteins, polypeptides, chemicals, cells,organisms, or compounds in the individual's biological sample. Themolecular data may also comprise, e.g., nucleic acid sequence data, andthe molecular profile may comprise, e.g., a genetic profile.

[0288] In yet another embodiment, the method of assessing the health ofan individual further comprises obtaining a second biological sample ora second health information at a time after obtaining the initialbiological sample or initial health information, processing the secondbiological sample to obtain second molecular information, processing thesecond health information, representing at least some of the secondmolecular information as digital second molecular data and second healthinformation as digital health information, and processing the moleculardata and second molecular data and health information and second healthinformation to generate a health assessment report. In one embodiment,the health assessment report provides information about the individual'spredisposition for vascular disease, e.g., CAD or MI, and options forrisk reduction.

[0289] Options for risk reduction comprise, for example, one or more ofdiet, exercise, one or more vitamins, one or more drugs, cessation ofnicotine use, and cessation of alcohol use, wherein the healthassessment report provides information about treatment options for aparticular disorder. Treatment options comprise, for example, one ormore of diet, one or more drugs, physical therapy, and surgery. In oneembodiment, the health assessment report provides information about theefficacy of a particular treatment regimen and options for therapyadjustment.

[0290] In another embodiment, electronically processing the digitalmolecular data and digital health data to generate a health assessmentreport comprises using the digital molecular data and/or digital healthdata as inputs for an algorithm or a rule-based system that determineswhether the individual is at risk for a specific disorder, e.g., avascular disorder, such as CAD or MI. Electronically processing thedigital molecular data and digital health data may also comprise usingthe digital molecular data and digital health data as inputs for analgorithm or a rule-based system based on one or more databasescomprising stored digital molecular data and/or digital health datarelating to one or more disorders, e.g., vascular disorders, such as CADor MI.

[0291] In another embodiment, processing the digital molecular data anddigital health data comprises using the digital molecular data anddigital health data as inputs for an algorithm or a rule-based systembased on one or more databases comprising: (i) stored digital moleculardata and/or digital health data from a plurality of healthy individuals,and (ii) stored digital molecular data and/or digital health data fromone or more pluralities of unhealthy individuals, each plurality ofindividuals having a specific disorder. At least one of the databasescan be a public database. In one embodiment, the digital health data anddigital molecular data are transmitted via, e.g., a communicationsnetwork, e.g., the Internet, to a medical information system forprocessing.

[0292] A database of stored molecular data and health data, e.g., storeddigital molecular data and/or digital health data, from a plurality ofindividuals, is further provided. A database of stored digital moleculardata and/or digital health data from a plurality of healthy individuals,and stored digital molecular data and/or digital health data from one ormore pluralities of unhealthy individuals, each plurality of individualshaving a specific disorder, e.g., a vascular disorder, is also provided.

[0293] The new methods and systems of the invention provide healthcareproviders with access to ever-growing relational databases that includeboth molecular data and health data that is linked to specificdisorders, e.g., vascular disorders. In addition public medicalknowledge is screened and abstracted to provide concise, accurateinformation that is added to the database on an ongoing basis. Inaddition, new relationships between particular SNPs, e.g., SNPsassociated with vascular disease, or genetic mutations and specificdiscords are added as they are discovered.

[0294] The present invention is further illustrated by the followingexamples which should not be construed as limiting in any way. Thecontents of all cited references (including, without limitation,literature references, issued patents, published patent applications anddatabase records including Genbank™ records) as cited throughout thisapplication are hereby expressly incorporated by reference. The practiceof the present invention will employ, unless otherwise indicated,conventional techniques of cell biology, cell culture, molecularbiology, transgenic biology, microbiology, recombinant DNA, andimmunology, which are within the skill of the art. Such techniques areexplained fully in the literature. See, for example, Molecular Cloning ALaboratory Manual, 2nd Ed., ed. by Sambrook, Fritsch and Maniatis (ColdSpring Harbor Laboratory Press: 1989); DNA Cloning, Volumes I and II (D.N. Glover ed., 1985); Oligonucleotide Synthesis (M. J. Gait ed., 1984);Mullis et al. U.S. Pat. No. 4,683,195; Nucleic Acid Hybridization (B. D.Hames & S. J. Higgins eds. 1984); Transcription And Translation (B. D.Hames & S. J. Higgins eds. 1984); Culture Of Animal Cells (R. I.Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells And Enzymes (IRLPress, 1986); B. Perbal, A Practical Guide To Molecular Cloning (1984);the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); GeneTransfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds.,1987, Cold Spring Harbor Laboratory); Methods In Enzymology, Vols. 154and 155 (Wu et al. eds.), Immunochemical Methods In Cell And MolecularBiology (Mayer and Walker, eds., Academic Press, London, 1987); HandbookOf Experimental Immunology, Volumes I-IV (D. M. Weir and C. C.Blackwell, eds., 1986); Manipulating the Mouse Embryo, (Cold SpringHarbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).

EXAMPLES Example 1 Detection of Polymorphic Regions in the Human THBS1and THBS4 Genes Variant Allele Discovery, Validation, and Genotyping

[0295] This example describes the detection of polymorphic regions inthe human THBS1and THBS4 genes through use of denaturing highperformance liquid chromatography (DHPLC), variant detector arrays,polymerase chain reaction (PCR), and direct sequencing. Cell linesderived from an ethnically diverse population were obtained and used forsingle nucleotide polymorphism (SNP) discovery by methods described inCargill, et al. (1999) Nature Genetics 22:231-238.

[0296] Genomic sequence representing the coding and partial regulatoryregions of genes were amplified by polymerase chain reaction andscreened via two independent methods: denaturing high performance liquidchromatography (DHPLC) or variant detector arrays (Affymetrix™). DHPLCuses reverse-phase ion-pairing chromatography to detect theheteroduplexes that are generated during amplification of PCR fragmentsfrom individuals who are heterozygous at a particular nucleotide locuswithin that fragment (Oefner and Underhill (1995) Am. J. Human Gen.57:Suppl. A266).

[0297] Generally, the analysis was carried out as described in O'Donovanet al. ((1998) Genomics 52:44-49). PCR products having product sizesranging from about 150-400 bp were generated using the primers and PCRconditions described in Example 2. Two PCR reactions were pooledtogether for DHPLC analysis (4 ul of each reaction for a total of 8 ulper sample). DHPLC was performed on a DHPLC system purchased fromTransgenomic, Inc. The gradient was created by mixing buffers A (0.1 MTEAA) and B (0.1 M TEAA, 25% Acetontitrile). WAVEmaker™ software wasutilized to predict a melting temperature and calculate a buffergradient for mutation analysis of a given DNA sequence. The resultingchromatograms were analyzed to identify base pair alterations ordeletions based on specific chromatographic profiles.

[0298] Detection of Polymorphic Regions in the Human THBS1 and THBS4Genes by SSCP

[0299] Genomic DNA from an ethnically diverse population (as describedby Cargill, et al. (1999) Nature Genetics 22:231-238) were subjected toPCR in 25 μl reactions (1×PCR Amplitaq polymerase buffer, 0.1 mM dNTPs,0.8 μM 5′ primer, 0.8 μM 3′ primer, 0.75 units of Amplitaq polymerase,50 ng genomic DNA) using each of the above described pairs of primersunder the following cycle conditions: 94° C. for 2 min, 35×[94° C. for40 sec, 57° C. for 30 sec, 72° C. for 1 min], 72° C. 5 min, 4° C. hold.

[0300] The amplified genomic DNA fragments were then analyzed by SSCP(Orita et al. (1989) PNAS USA 86:2766, see also Cotton (1993) Mutat Res285:125-144; and Hayashi (1992) Genet Anal Tech Appl 9:73-79). From each25 μl PCR reaction, 3 μl was taken and added to 7 μl of loading buffer.The mixture was heated to 94° C. for 5 min and then immediately cooledin a slurry of ice-water. 3-4 μl were then loaded on a 10%polyacrylamide gel either with 10% glycerol or without 10% glycerol, andthen subjected to electrophoresis either overnight at 4 Watts at roomtemperature, overnight at 4 Watts at 4° C. (for amplifying a 5′ upstreamregulatory element), or for 5 hours at 20 Watts at 4° C. The secondarystructure of single-stranded nucleic acids varies according to sequence,thus allowing the detection of small differences in nucleic acidsequence between similar nucleic acids. At the end of theelectrophoretic period, the DNA was analyzed by gently overlaying amixture of dyes onto the gel (1× the manufacturer's recommendedconcentration of SYBR Green I™ and SYBR Green II™ in 0.5×TBE buffer(Molecular Probes™)) for 5 min, followed by rinsing in distilled waterand detection in a Fluoroimager 575™ (Molecular Dynamics™).

[0301] Direct Sequencing of PCR Products

[0302] To determine the sequences of the polymorphisms identified asdescribed above, the regions containing the polymorphisms werereamplified using the identified flanking primers. The genomic DNA fromthe subject was subjected to PCR in 50 μl reactions (1×PCR Amplitaqpolymerase buffer, 0.1 mM dNTPs, 0.8 μM 5′ primer, 0.8 μM 3′ primer,0.75 units of Amplitaq polymerase, 50 ng genomic DNA) using each of thepairs of primers under the following cycle conditions: 94° C. for 2 min,35×[94° C. for 40 sec, 57° C. for 30 sec, 72° C. for 1 min], 72° C. 5min, 4° C. hold. The newly amplified products were then purified usingthe Qiagen Qiaquick PCR purification kit according to the manufacturer'sprotocol, and subjected to sequencing using the aforementioned primerswhich were utilized for amplification.

[0303] Case-Control Population

[0304] A total of 352 U.S. Caucasian subjects with premature coronaryartery disease were identified in 15 participating medical centers,fulfilling the criteria of either myocardial infarction, surgical orpercutaneous revascularization, or a significant coronary artery lesion(e.g., at least a 70% stenosis in a major epicardial artery) diagnosedbefore age 45 in men or age 50 in women and having a living sibling whomet the same criteria. These cases were compared with a random sample of418 Caucasian controls drawn from the general U.S. population inAtlanta, Ga. Controls representing a general, unselected population wereidentified through random-digit dialing in the Atlanta, Ga. area.Subjects ranging in age from 20 years to 70 years were invited toparticipate in the study. The subjects answered a health questionnaire,had anthropometric measures taken, and blood drawn for measurement ofserum markers and extraction of DNA.

[0305] Statistical Analysis

[0306] All analyses were done using the SAS statistical package (Version8.0, SAS Institute Inc., Cary, N.C.). Differences between cases andcontrols were assessed with a chi-square statistic for categoricalcovariates and the Wilcoxon statistic for continuous covariates.Association between each SNP and two outcomes, CAD and MI, was measuredby comparing genotype frequencies between controls and all CAD cases andthe subset of cases with MI. Significance was determined using acontinuity-adjusted chi-square or Fisher's exact test for each genotypecompared to the homozygotes wild-type for that locus. Odds ratios werecalculated and presented with 95% confidence intervals.

[0307] Genotype groups were pooled for subsequent analysis of the toploci. Pooling allows the best model for each locus (dominant,codominant, or recessive) to be tested. Models were chosen based onsignificant differences between genotypes within a locus. A recessivemodel was chosen when the homozygous variant differed significantly fromboth the heterozygous and homozygous wildtype, and the latter two didnot differ from each other. A codominant model was chosen whenhomozygous variant genotypes differed from both heterozygous andhomozygous wild-type, and the latter two differed significantly fromeach other. A dominant model was chosen when no significant differencewas observed between heterozygous and homozygous variant genotypes.

[0308] Multivariate logistic regression was used to adjust for sex,presence of hypertension, diabetes and body mass index using the LOGISTCprocedure in SAS. Height and weight, measured at the time of enrollment,were used to calculate body mass index for each subject. Presence ofhypertension and non-insulin-dependent diabetes was measures byself-report (controls) and medical record confirmation (cases).

[0309] Results

[0310] In the population screened, one SNP in THBS1 (G334u4) and one SNPin THBS4 (G355u2) emerged as important SNPs associated with CAD and MI.G334u4 is a change from an adenine (A) to a guanine (G) in the THBS1gene at residue 55322 of the reference sequence GI 12583762 (THBS1).This polymorphism results in a change from an asparagine (N) to a serine(S) in the amino acid sequence of THBS1 (SEQ ID NO:2) at amino acidresidue 700. G355u2 is a change from a guanine to a cytidine in thereference sequence 14916146 at nucleotide residue 105290. Thispolymorphism results in a change from an alanine (A) to a proline (P) inthe amino acid sequence of THBS4 (SEQ ID NO:4) at amino acid residue387.

[0311] For CAD, 146 of 342 subjects carried at least one copy of theTHBS4 (G355u2) variant allele compared with 140 of 406 control subjects;odds ratio 1.42, p<0.03. For premature MI, the association was evenstronger: 90 of 184 cases vs. 140 of 406 controls had the variantallele; odds ratio 1.82 p<0.001. The THBS1 SNP (G334u4) was rare.Nonetheless, homozygosity for the variant allele gave an odds ratio of7.15 (p<0.08) and 8.76 (p<0.07) for CAD and MI, respectively.

[0312] Several other SNPs were identified in the THBS1 and THBS4 genes(see Table 1, below). A second polymorphism in the THBS1 gene is achange from a thymidine (T) to a cytidine (C) at residue 53502 of thereference sequence GI 12583762 (polymorphism ID No. G334u4). Thispolymorphism does not result in a change in the amino acid sequence ofthe THBS1 gene (it is a “silent” variant). A third polymorphism is achange from a cytidine (C) to a thymidine (T) in the THBS1 gene atresidue 60793 of the reference sequence GI 12583762 (polymorphism ID No.G334a18). This polymorphism is not present in the coding sequence of theTHBS1 gene, and therefore does not result in a change in the amino acidsequence of the THBS1 gene (it is a “non-coding” variant). A fourthpolymorphism is a change from a thymidine (T) to a cytidine (C) in theTHBS1 gene at residue 58445 of the reference sequence GI 12583762(polymorphism ID No. G334u5). This polymorphism is a silent variation. Afifth polymorphism is a change from an adenine (A) to a guanine (G) inthe THBS1 gene at residue 52861 of the reference sequence GI 12583762(polymorphism ID No. G334a16). This is a silent variant. A sixthpolymorphism is a change from a thymidine (T) to a cytidine (C) in theTHBS1 gene at residue 49556 of the reference sequence GI 12583762(polymorphism ID No. G334k2). This is a non-coding variant.

[0313] The second THBS4 polymorphism identified is a change from aguanine (G) to an adenine (A) in the THBS4 gene at residue 118019 of thereference sequence GI 14916146 (polymorphism ID No. G355a11). Thispolymorphism results in a change from a glycine (G) to a serine (S) inthe amino acid sequence of THBS4 (SEQ ID NO:4) at amino acid residue718. A third THBS4 polymorphism identified is a change from a guanine(G) to an adenine (A) in the THBS4 gene at residue 89835 of thereference sequence GI 14916146. Although these SNPs were not associatedwith CAD and/or MI in this population, it is expected that they may beassociated with CAD and/or MI in other populations. TABLE 1 8 3 4 5Genbank 9 10 1 2 var Type of Geno- 6 7 Accession/nt Flanking SEQ ID GenePolyID freq var types Ref Var position sequence NO: THBS1 G334u4 0.1 MisGG A G GI 12583762/ CTGGCCCA 5 (NIS) GA nt 55322 gTGAGAACC AA THBS1G334u3 0.1 Silent CC T C GI 12583762/ CAGATGAA 6 CT nt 53502 cGGGAAACCTT THBS1 G334a18 0.15 Non- TT C T GI 12583762/ ATGGGCTTGAG 7 coding CTnt60793 AAAA CC tCCCCAGGATC ACTTC THBS1 G334u5 0.15 Silent CC T C GI12583762/ CATCAGTGAGA 8 CT nt 58445 CCGA TT cTTCCGCCGAT TCCAG THBS1G334a16 0.11 Silent AA G A GI 12583762/ TCAGGAGTGTG 9 AG nt 52861ACAAaAGATGT GG AAGCATCTT THBS1 G334k2 0.11 Non- TT C T GI 12583762/CCTCTGCACCC 10 coding CT nt 49556 AGCCtGTTAGC CC ATGAACCCT THBS4 TSC06560.50 Non- AA G A GI 14916146/ GAGAGCCGAGC 11 290 coding AG nt 89835ACCCaCTGACT GG GACAGCCTT THBS4 G355u2 0.19 Mis CC G C GI 14916146/GAAATGGA 12 (A/P) CG nt 105290 cCGTGCGTT GG THBS4 G355a11 0.02 Mis AA GA GI 14916146/ AGACCGTGaGC 13 (G/S) AG nt 118019 CTGGAT GG

[0314] All of the SNPs in the THBS1 gene were found to be in linkagedisequilibrium with each other (see Table 2, below). G334a16 and G334k2were found to be in negative linkage disequilibrium with G334u4. Oddsratios for the variant allele of each of these two SNPs show aprotective effect, e.g., subjects with the variant allele are lesslikely to have vascular disease. This is consistent with the G334u4variant as a risk allele G334u4, i.e., the variant allele of G334u4 hasbeen shown to be associated with increased risk for vascular disease.Any of these SNPs (e.g., G334u3, G334a18, G334u5, G334a16, and G334k2)can act as markers for G334u4. TABLE 2 OR CAD OR MI D' with P vaulue SNPhomozygotes homozygotes G334u4 for D' G334u3 1.99 2.20 .96 <.0001 G334u51.19 1.25 .97 <.0001 G334a18 1.02 0.93 .97 <.0001 G334a16* 0.49 0.45−.97 .02 G334k2* 0.64 0.60 −.97 .02

[0315] The three SNPs in the THBS4 gene were in linkage disequilibriumwith each other (D=1.0, p<0.0001) (see Table 3 below). Therefore, eitherof the G355a11 or the TSC0656290 SNPs can act as a marker for the G355u2SNP. TABLE 3 OR CAD OR MI D' with P vaulue SNP carriers carriers G355u2for D' G355a11 1.04 0.97 1.0 <.0001 TSC0656290 1.18 1.07 −.93 <.0001

[0316] Equivalents

[0317] Those skilled in the art will recognize, or be able to ascertainusing no more than routine experimentation, many equivalents to thespecific embodiments of the invention described herein. Such equivalentsare intended to be encompassed by the following claims.

1 13 1 185695 DNA Homo sapiens 1 aagcttcctt tgtagaggag aatgagtacttactgcttac aagacaagca gcatgttttt 60 caaaagtcaa aagaaagctt gtgaggaacaaggtaatact ctctagatag ctggccttgt 120 cttatgcaca ttcattaatt cattttgtgcacagaaataa catgtccatg aacctggggg 180 aaccactctt ttgagagacc ttgatcttggagttcctctg actcaatatt aggagcaaga 240 tagagtaaga cacctatttt tttatgtttgccattacata agaatgaatg aaacaaaggc 300 atagttctgc attggttaat aaaacaaaaagcccgctaaa aggcaatgtt atatattcgg 360 caatcacatt ttgaagacca actatattcaagactcatgg caggttctgc atgccactga 420 tgcatctgca tgacctttcc aaggctatttgtcacagcag ccccgaaatc gtggcctcta 480 agggcctaag tgttctggag taaataggccaggccagcac ctctgagaaa taggggctca 540 ccccatcctg agccaccttc ccagtctctgagcaacctca gcatcagtgg ccctctgacc 600 gcatcagcct gtgggtccct acttgtctgaacacatacaa ttttctttct tttaattggc 660 aagctttgaa aaacaagtac atgagtgaacacagagcaaa gagtttagtc tgggagctat 720 ttctcgtaag tctcttttga gaaagagtttctagcccttc cccactttca tgagattaga 780 gagataagaa caggaacatg gagggaggggtccacactct ggaaaacact tttggatctg 840 ataaccaccc taatgaagcc ccaaagctatcgcctttgga tgggtttctc aaacatggaa 900 ttctaccagg atttctgtca acaggcctcctaatcccctc tcgcctgcca agccacatgc 960 tacacaaagg tgaagaacaa gaaaggcattgagacacgtg ttctatccag ccaggggatg 1020 cacaatgtct gcccacgcag gctgcgttattatcagcagg aactgacaga ggggtctgtt 1080 ctcccctctc caggcagtgg aggggacgcttcccacctct gacctgtggc tgagcccagc 1140 ccgagcagaa atcccagaag aggtcccggccttgctcgca tcctgcttcc tgtgctaaga 1200 cctggtcgga atgaaagata gtaaaagttctttcacattg ggaaggcctt tacaaagctt 1260 gcctttacta ttttataatc tctacattttattgtccttt taaaatcaca aaagcaagca 1320 gaaacatgag gaggctgctt tatttgctctcccattcaac aactgggctg ctgagttacc 1380 agttccccct actacttgat ttggaaatggacactagaaa tttttcccac ttgtgactga 1440 agctgcagaa acttcaacct gttttcagttaccctgttgt acctaataag agtccatcct 1500 cttttcagtg gaaagaaagc catggacgaggcattgcagg tctagtgtgg ctctcagact 1560 ctctgggagc agctgacgca catttaagaacagggagtcc ctgaagggtg gctgaggaca 1620 gatcacgaga ccagaggtga gaggcgcccgacagggttgg agccctactt ttgggtgtga 1680 gcagtggggt ctgatgcacc acggtgaaggcattcagcag ccaggtcctg agtagcagaa 1740 acacaggata cacagagggc aatgtcttagagccacacca actgggtagg actgaacaga 1800 tggtcattca acagatgata cacctttagggtgtatcagc aatccattgg aaaacttgtt 1860 aaaaggcaga ttctagctca gccctagattctgatccagc aggcaggaat ctgcatgttt 1920 ccagtttttc cagctgattc taatgtagatggactctgga ttgccatttg agagacactg 1980 atgtagaaca agatcggtta gactggagacattggccaag gtaatttcta gagaaaatgt 2040 gcttgctcaa agtcctgagg acaaatttaagatcagagtc tagagaaatg aacatgaaca 2100 tactcacagc ttaaaggcta ggttgggaggcccaaaggaa gcacatgttc aaggcacaag 2160 cgagtcatta cagtgaacaa gcctcagagaagcttctgga agcccatgat ctggcagatg 2220 gcacacacaa tagtatggca gatggtacataccaggtaaa ccctaaatgc ataaggttcg 2280 tattacacgt aggaaaacaa ggcataagcctaagcaaggg gcctggcccc tccaaatcta 2340 caggtaatgg gcagagtcct ttagaagcatgggtgcatta gggaatttta ccttgcctca 2400 acagaaacta tggtaagcgt aggtgctgcaaggaagccat acttcaattg ctctacatct 2460 attattccag caaagatgat gctaggttctatgagaaatt ctaaagacga cacagctctg 2520 ttgtcaaaga gcttacgata taatcataaagcactttaga aaacttggct atttaagtaa 2580 gcccaaatac tggataatat tgaagcagttgactgcaatc tagagtacaa agtgggtcct 2640 gtgcagccag gaaaaagctc acagagtaggcagagggtcg agactaagtg atttaaagcc 2700 ctcataattc tttctgcatg gcccctttttccagcaggcc tgaccatgtt ctatgcaatt 2760 cacaccatct gaaaatacat cgcagcccccctgttcccct cacagcatta gttctcacct 2820 tgaagcttct gggactcaca ttcagcttcttgccctgttc ctagtctcat cttctgatac 2880 tccaccttcc ctgttaaggt tttgccatgcccttgaacat gacattagat acaactccag 2940 ttttgactct tactaccctc tctctccccattcacggcac aatgatgatt tgcaaactcc 3000 ctccagccac cttggatata taatcacggtccctgatcca accttgcccc tgacctttgc 3060 atccttgtac cttggtatgg caccttgtacctccatgggc agcagctcca tgggaatttt 3120 ccagtcccag agaattcatg cattcatgaagggaaagaac aaatgggatg agtatgaagg 3180 cagaaaggag agttggaaga taaggagcaatagtcagaca ggagaaccta cattttgaga 3240 tcaacgataa tgtcttccct ctcaatcaggatacttctta atctcaagta ctctattggg 3300 aaggaagaga tcagctgttt ctgggctcaaataaaggtaa tctttggctg gaagcactag 3360 gcaagcacag tcttctacat cttgatggagcctcatagga aggtggaggc taaagcacaa 3420 atgacatgta aaggaaagtc tggtgagattgagcattagt ggaagaactt tggtggcctg 3480 gcctgatttt cacctgagcc aggttgctggaggcgtgttc ttgctgagtt tcatctgtgg 3540 ccccaaagcc tttggggatt cagaaccaatgggtactctg cagtctggcc tccacatgac 3600 ccccaactgg ctttgtgatg aaaggaatcatgttccatgt aagaacccat cttctgaaca 3660 ccccactgca ctctcactgg tagaagacaaatctgatgct ctagggagtt atatagttta 3720 tttcttctca tttcaacctt tctatttctcagacacatat gcacaggtgc acacacacac 3780 aagtgcacac acacacgtgc ttgtgcacacacatacacac acagaggact ttgaactgcc 3840 taagggcaag aataattccg tattcatgttctacaatgcc tggagaaagc ttttcacaca 3900 gtaggccttc aaaaaattcc actaatgagcaggattcata atagtaaagt aaatgatgct 3960 gctacaaatc tgagccatgg gaggatccatgaagaatgcc aggtacgaat atcttcaggg 4020 tccgtctcat agggatgcag tcccatagatgtctataagc cccttaactc acagcctcaa 4080 attaatcaag caaagagact aagagaaacttgtcaagtct tgtatctcct taattttacc 4140 ttatgctaag tttccatgac agggagcagctatgactggt cagcagtcac cagccatagt 4200 ccagcccttg gaggcttgga gatctatagcctagattgaa gagtgtggaa atggctagga 4260 aagtaaggat aggtaagaat tatctattgtatctactcta taaaattata gaaaagccac 4320 tcttcacttt cccaagaacc cctcattgataaagatttgc agaaagtgtc tgtctggggt 4380 ccctgttgct tcctttactt gtcctgagtcagccacagct gtttccctcc ttataatcat 4440 ctcattgtca ttggatggca gcagagactttatgagaaag ctcatgtggc tacggccctg 4500 cctcctttgc acgaggataa gaggtttcaagaagaccttt ttcacaaggg cttgtccagg 4560 atttgggcca gtcccacagc attccaggtccttgaagtct ggctgaaaac cttgtgggaa 4620 gagaaaacct tctaagcaag agaaagccaacatatattaa aatgttcttg tcttcccgga 4680 taggttgtgt tttgtgaata gggcaatatgtcatttctcc caggaaaaga aaacacaaaa 4740 ctgattcaaa atgtactgag gtaaaaagagttgcagaaga aaattaatct tcagattccc 4800 taactttttg tgtcccataa ataatctgtggccttgtccc agagagaaat gtgtctgcca 4860 ttttggcaaa ccaggtatct gagagagagaatagagttgc cctgtcttga ggaaagacca 4920 caaggcaaac ataaatgaaa agaacaaagactctgtattg ttgagctgaa tggccaccca 4980 aatatcccaa agaatgaact ttaactttctcaacagaaag gcaaccacag gaagtttggc 5040 agctcatgga gaaagttcct tcatgaatatgtggccttta cagtgcttcg tctttacaca 5100 gaattaactc cgggcctgaa agatgtgtgccttttcctgt tttgttttaa tggcagactc 5160 agaacacagg gtacagtttt gcttctgtctttaaagttgt ccttgctttg ccctccaagg 5220 cctgtagcca gtcgagcatt tttggcaagacagaaacact ctccagtccc aagaatgccc 5280 aggaaccaac agacatagat ggttcctaagcttatttcct gtgttcagat tcaagagtgg 5340 aatcccagtt tggcctggct acagaggctggaaggtagaa aaggtgaaaa attctctaaa 5400 atgtttccag gttttctatt tactggataagcaacaaacc aatagctgtt tggagtagct 5460 agctggctgg cagcaatttt ccaagaatttccaaatagca gtacgtggcc taagaaaggg 5520 aggtaccagg aaaggagtaa gaagggagcaaagtaaaaaa tgatttattg gtgcccttgg 5580 caatataaag ttgaagtgga cagcgcagccaaaagtatct tgctactgca gtagggaaat 5640 tagccaaggg tggtgtcaat tagagatacaaggggtggta atttcactgg agaagccaac 5700 tctaggtatg ggtgctgagc aattgccccagcaacctgcc aaggtatgtt ctatttgttt 5760 tcaccaggac tgtggatagg ggtgtgtgtgcgagagagag agagagagaa aaaaaaaagt 5820 aggtcatcct atcaaaagag aaggtgaaagggttgaggaa ggcctctctt ctctctgatt 5880 tattgtagag caggaagagt cactgtctgcaatacacaaa ataatacatc aggaaaacag 5940 gaagtcaaac tttcaagaat gcaaggggaaatcaaaacca gagataggac aaggatgaat 6000 gaaaacacct ggcatagaag tgaaacgagaaaaaaggaaa cttttgcttt cttggaaata 6060 cacaatcggt ctgagcctca ttctttggaagttaagtcca gtccttctga aaaagatgtg 6120 tggaccactg aagaggaacc atgatgataattcaccccct gcaggctgtg ggagaggagg 6180 tttggaagac aggtaggaag ttggctctctgaataatgta gacaatgagg aggtacatcg 6240 tcctttgaga ggtgataagg aggcggccatcatttagcaa aattctatgc ctcctaatga 6300 tagatgtgct taggtatttc tataccaatggttgtaatga aaagagtata aacttgagtc 6360 aaacagaaac aacttttctt tgtgtatacaacaggaataa tgctgattac cccaatcttg 6420 gctacagagt tagaatgaaa tggcacctggaacataatag atgttctttg gatttaattt 6480 attgtctttt cctcttcatg ccgagataaaagagtaccag tagaagaagt ttgagacaca 6540 ttctctagag acagaaaatg taaatggcataagatgatca gtggctttac ccattgattc 6600 ttccaacgaa aggtcgggtg taatggacaagaaaggaagg gatgagaaac aaaagacagt 6660 ggacaaacgg aattcaaaac cagttgtgtatttctagttc tggatttcag acactaaatg 6720 tagagcctct agttaagagt ggaacatctctcacaatgcc tggagaccaa atgaactaat 6780 tcagaagctt taactaatgt gaaagaaatgccagataaaa aaatgaagag aagctattaa 6840 gaataagaga tgtggcaaga aatataaagttggaaaggtg atcagtgaac cacatgaaga 6900 aataactaaa aatcaggcaa aaacaacactaatgaactca gatctctatg ttccaatgtc 6960 agagcatgga caacaaaaaa caagattccaacattgaatg gaaaatctga tctcaaaagc 7020 ttcaccagtg gccaggtgtg gtggctcacacctgtaatcc caacacttta ggaggccggg 7080 actggcagat ctggcctgag gtcagaagttcaagaccagc ctggccaaca tgctgaaacc 7140 ccatctctat taaaaataca aaaattagccaggcatgatg gtacacacct gtagtcccag 7200 ctactcagga ggctgaggca caagaattacttgaacctga gtggtggagg ctgtagtgag 7260 ctgagatcat gccactgtac tccagcctggccaacagagt aagactctgt ctcaaaaaaa 7320 aaaaattacc agaacttctt ggaatggagctcaacattga aatattgcaa acatgctgag 7380 aagggaagac aaagtagtta taggcctaaataatgctctg tgttgctgat ggcattactc 7440 acacactgta ggcagagata taaaatggtacatatttcat agagggtaat tgaaaatatg 7500 tagtgagtgt tctgatgtgc catcagactcttccttcaga accaagggag ccattctcct 7560 agttgcaaga ggatagcctg ctgcattggtttctgatagc tgctctcaca aatattacca 7620 caaatttcat ggcttatgtt ttaggcaaatgctaaaactg tcttaaacaa atgtattaac 7680 ttatagttct aggcatcaga agtctgaaatgggtcttgtg tggctaaaat caagacgctg 7740 gtagagctgc attcctttct ggaggttcttgggaagaatc tattttgttt gtttctgttg 7800 gttttctggg tttgtttttt ttttttactttttttttctt ttgtcttttc cagcctttag 7860 aggctgcctg ccttcctcgg ctcatggccccattccatct tcaaagttga atctttctta 7920 cattacgtca ttgacaccct ctcccttctgccttcctctt tataaggatc cttgtaatta 7980 tactggacct acctggataa tccagtataatatctacatc ttcatttcat ctgcaacctt 8040 cattcttctt tgccatgtaa tctaacatattcatagattt caggaattag gatatagaca 8100 tctttggggg ctgttattct acccgccatgtctgacagct gctcacagct gagtctctcc 8160 ccaggagtta tctttggttg aaagcagctgcctcacacaa ggttatgctc cacaggaaaa 8220 actggcatcc aatgactggc tgatttgaaggcccaaaggt ccagcccccc tcaatgcctc 8280 agtatggaac aatcctcact agccatcccagctccagggt gcccttgaat caactgaagc 8340 cactgctgca atgtattcca aaattcagcttttttctggg actagtcttg taattttagg 8400 tccctccaag ttgttgttct caagagctctctccaatatc ttcctgctaa caaatctctg 8460 tctcaaagtg tttcatggga aaaccaacctaaggcagtgg atgtcaggaa tggtcagagg 8520 aagctgactc taaaatagaa cttaggagatgggcacctcc tagcccatta acaatgagga 8580 ccccatcact agtgaaaact gaagtttgtatggccctcac atgttatact ggagtgttaa 8640 aatattcctt ggtggtcagt gggatgggatataagtggaa ggaaatgcac caaaggatgc 8700 aatatctttg acatttgagc cattctggggaaactgggat tgctgatgct gaatattatc 8760 tatgtgctgg gaaaaaagaa gggaaatctgggaaagggga gctgatcaat tgtctattga 8820 aatcaaattg tgatagccag aggacttccttggcagcact aaaaagtgac tctcatcttg 8880 tgtagtttga aagcagaaaa atctgagaatcaggccaacg acttaattat aagtatagta 8940 caactcaaaa aaggtagaat tcttaatcccagcaaatctt gaaactccag atcccctcag 9000 aaccatttgc tcctgtagaa gtgatgcactcctcttcaag taaagacccc ttagttgaag 9060 acaacacagg gttctctgcc tgtaaaacagtatgtcttct cttgggattt ggctcacatc 9120 taccctcctg gccactagag agtcgagttgcaatagaagc tggccaggaa agtgctgagt 9180 cagctaggaa aataaagagc ccaaaccccaaaggggttgt aggtcccagc caaaatgtgc 9240 cagcagaggt cagtaaactg taaatgggactaaagcctga gaagcctgga tcaaggggag 9300 tataatacaa atttgaataa gggagagattgttgctgtga agctactctc ttacaataca 9360 gggttcaact ctggtaggca cccaggcagatggtggttaa tgttctgcta gcatagctct 9420 tggaaactac catattctac cataatatattataaaagaa gaaaccaaaa gactcaaagg 9480 ggtgggcata aaatgtagag ataaaatgggagctggaaaa cctaccagca gatgatgttc 9540 taagacatat cagaaagaca ttgtgctcaccagagcgatg agagaggtaa tgggagcaca 9600 gcagcaacag cgtcaagaaa ctcagtgttgcctgtcctct gtaggccaag ctgattataa 9660 aaattgaaaa gtgggccagg catggtggctcgtgcctgta atcccagcac tttgggagcc 9720 tgaggcaggc ggatcacgag gtcaggagatcaagaccatc ctggctaaca cggtgaaact 9780 ccgtctttac tgaaagtaca aaaaaattagctgggcgtgg tgacgggcgc ctgtagtccc 9840 agctactcag gaggctgagg caggagaatggcgtgaaccc aggaggcgga gcttgcagtg 9900 agccaagatc gcgccactgc actccagcctggggaacagt gcaagactcc gtctcaaaaa 9960 aaaaaaaaaa ataagaaaaa aattaaaaagtgataatcct ttacccagtt tccaggacta 10020 aaccagtttt cagatcttcc agtgtttgactgaaggagag accaggtacc cagaaggaac 10080 agctctgcag tgccacagca agtgtgtaaggtaatgactg cctcagtttt tgcctaaagg 10140 acctatagcc gtttactcaa gtaactctaaactgaggaaa ggggaatatc ctgacacttt 10200 gaggttgatg gatgcagggt caaaaataacatggatacct agggaactga agcatcatca 10260 tgcttccctt gttagaggac ataaagagggcagataatag agctgggcaa gttggaccaa 10320 tgaatcttac agtgggtttt ttcaaaagataaacaaaatt gacaaaactt taattagatt 10380 aaccaaggaa aaaagaaagg actcaaaaaaaattacaaat gaaaggaggt attaaaactg 10440 ataccacaga tgtacaaagg atcacaagagactatgatga acaattacac accagcaaat 10500 tggataatct agaagaaatg aagcatacattcctagaagc atacaacctg caaagactga 10560 ctcatgaaga aatagaaaat ctaaacagaccaataagaac taaggagatt gaatcagtaa 10620 tcgaaaacct cccaacaaag aaaatcctagaaccagatgg cattattggt gaactcaatc 10680 aagcatttaa atgattaaca acaatccttctcaaattctt ccaaaaaaat gaagagaagg 10740 aaacacttcc aaattcattt tatgaggtcagcatcacact gataccaaag ccagttaagg 10800 atgctacagc aagataaaat tacaggttgatattcctaat gaatgtagac acaaaaattc 10860 tcaacactag caaaccaaat tcaatagcacactaaaagga ttttatgcca taatcaagtg 10920 ggataatccc aggaatgcaa ggatggttgaacatatgcaa atcaataagt gggataaact 10980 acattaacag aatgaagatt tatgatcatctcaatagatg tttaaacaaa agcattcaac 11040 aaaactcaac attgttttat aataaaaactctcaaacaag ttgggtacag aagaaatata 11100 cctcaacata ataaagctat atatgtcaagcccacagtta acattatact caaatgatga 11160 ataatcagga acaagataag ggcacccactcccaccacta ccaatattat tgaaacttat 11220 agccagagaa attaggcaag aaaaagaaataaaagtcatc taaattggaa aggaagaaat 11280 aaaattgtct ctttgtagat gatgcgatcttacacattta gccagaaaaa cattagaact 11340 aataagcaaa ttcagtagag ttgcagaaaacaaaatcagc acacaaaaat cagtgtgttt 11400 ctatataata acaacaaagt atcagaaaaagaaattttaa aatatgttat ttacaataga 11460 atcaaaaata aagtatctag gaataaatttaatcaaggag agataaaaga tctgtacact 11520 gtacataaac ataaaaagat ctgtacactgtacataagga attgatgaaa caaactgaag 11580 aagacataaa taggaaggta acccgtgttcatggattttt aaaaattaat agtattaaaa 11640 tgtctatact acccaaaata atctattgattcaatgccat tcctatccaa attcttcaag 11700 catccttcaa agaaatagga aaaacagtcctaaaatttgt atggaaccac agatgacccc 11760 aaaaaagcca aagcactctt gagaaagaacaaagctggag gtattacatg tcctgatttc 11820 taatcatatt acaaagctaa agtaatcaaaacagttaaaa cagacacata gatcaatgaa 11880 acagaataga gagcccagaa ataaacctacacatataggg taaactaata tttgacaagg 11940 gtaccaggaa tgcatattgg ggaaagaataatcattttca tacacagtgt tgaaaaactg 12000 gatatccaca tgcaaaagaa tgaaactggacctctgtctc atagcataaa aaaaaaaact 12060 caaaatcaaa acacaaatat aagatctgaaaccataaaac tcctacaaga aaacgtagaa 12120 aaaacttcct tgacattggc ttcataattacttttttgat atgacaccaa aacacaggaa 12180 acaaaagcaa aaataaataa ataggactacatcaagctaa aaagcttatg catgacaaaa 12240 gaatcagcaa aatgaaaagg cacctatagaatgggagaaa atattggcaa accatgtatc 12300 tgttaagggg ttcatttcca aaatatataaggaactcata caactcagta gcaaataata 12360 ataataattt aactaaaatt aaattaaatttcaatttatt aaattaatac tcaaattaat 12420 tttaatgtat taaattaaca gcataattaaaatggtaaaa agacctgaat agacactttt 12480 ctaaattcat aaaaatgtcc aacaggttcatggacaggtg ttcaacatcg ctaaccatca 12540 ggcaaatgca aatcaaaact aaaataagacatcaccatat acctgttcgt atggctatta 12600 ccaataagac aagaggtaag tgttggtgaggatatagaga aaagagaaaa cttgtgtacc 12660 cttggtagaa tgtaaattgg cacagccgttatggaaaaca gtatggtgtt gcctcaaaaa 12720 attaaaaaaa gaactaccat atgacccaccaatcccactt ctgtgtctat atatccaaag 12780 gaaataatat caatctctca aagagatatcttcactcttg tgttcactgc agcattattc 12840 ataatagcca agacatggga acaacttaactgcctgtcaa caaatgaata aagaaaatgt 12900 ggtatacatg tagttaaata ttattcagccataaaattgg agaacatctt gctatttgcg 12960 acaacatgga tgaaccggga gggcattatgctaactgaaa taagccagaa aaatgaggac 13020 aaatactgta ttatctcaca tatggaatctaaaaaagtca cactcaccaa aacagaaagt 13080 tgaatcatgg tttccagggg ctggaggttaggggaaataa gagtatgttg gtcaaaggat 13140 acaaactttc taatataaga tgaataagctctgaggatct aatgtacagc atggtggcta 13200 tatttaatga tactgtattg tgtatttgtaatttgctgag aatagatcct gtgttctcac 13260 cacaaaaaca aaaatggtaa ctatctgaggttagggatat gttaaccaac ataattgtga 13320 taatcattcc ttcacaatat atacttatatcaaataatca tgttgtatat tttaagtgta 13380 tacaatttta tttgtcaatt atacatcaacacaagtggga gggaaaaaac gagaaaattg 13440 catgagctaa tgacccaagt cctcatgtcattcaccactg ttgcaccagc tatggctata 13500 tgggcaccac ttatgttcag ctgatagaggaagggaaaag ccaagctttg ttcaaaacag 13560 ttgggccagt ttgatgtagg aatacaaaccagaaaaggtc tttagtattc tcagtcccac 13620 tcatgaataa ccctaaaaga cagcagtgaggggacatcct cccaatggac ttcaagtgat 13680 gcacctggcc actcactttg tgtggaaagtggctcaaggt caaatatatc taaactcatg 13740 ggcaattgta aatgggttgg ctggttgcttagtggcctgg aaggagaaag agtgaaagat 13800 aaggaataag gaggtctggg gaagaggaatacggaagtgg acatgaagtg caaagagctt 13860 catgaagatc tttgtatacc atgttaacgcctaccatgga agaggcaatt tctagtcaaa 13920 tggacagact gatttggcca ggtgatgtcagacagccctt tctcattaat caccccagtg 13980 ctggcaagca ggcatatgaa cagagtaatcatggtggcag gcacggaggc tgtgcaaggc 14040 agcttacaaa ataggtgatc tagtttctgctgctgctgaa tatctatcag gaacagagat 14100 tactgccgag cccctgacaa agaacaattcttcagagagt ccaatcggcc atgtggtggc 14160 acagcaatta cattaaacct ctttctccatggaagggcca gcaattcatc ttgaaggaaa 14220 tcaacacata cttaggatac aggtttgccattcctgccca tagatcctca gccggcacca 14280 ttatctaagg acttacttca tgtacgattcactaacacag gagcccacag aacatcctgt 14340 tgtacccaga aactcacttt agaagaaaagaggtacaacg atgagcacag gaccagctgc 14400 tgactttata aggtaaagga acacccttttgaaagtattg ctgaagtacc acattcaata 14460 taatacttaa ggctggagtc cttaagcatcccgcatcctc caggatgcag tgtccaccct 14520 taataataag tgactattat acagtactgtgtctcctcta tatagattac atggttcagg 14580 gtggaaatag ggctaatccc tcctaccatctcactcaata acctacccag ggaattcatc 14640 ccttccatct ccacgactct gagctctgcagctctagaga gtctggttcc aagaacagaa 14700 acacttatac caggaacact acacgaatcccattaaactt taagctacag ctgttggcac 14760 tttatgtttc ttgtgccaag aggtcaacaggccaaaaaag gcatcaccat tttggcagtg 14820 gtaattgatc aggagaaggt tgagctattgttacacatgg tgacagaaaa gactatttgg 14880 ctcccagaag gacttcttgg gcatctcttggtaataccct agccaatcgt aatggtaaat 14940 ggataaaagt agtagtcatg gcttgagaagggcatgatga ctacaggctc agatccttca 15000 tggatgaagg tttgggtcac ccaaccaggtaagccacctg gagcagagct ttcagccaag 15060 gggaagagaa ctctacactg ggttgtaaagaagggatttg atgagtgtca gttgtgtccc 15120 tgagaacagc tgcattgttg agagctacagtttgtcccat taatcttcct cttgtacatt 15180 tccctaggaa aagaagcaaa ccagagttctagaggagctc ttcccagata gggtgatttt 15240 tttaaataag aaatatgtgg tccaagagttgaaaggggta actgaaacaa tcactttgat 15300 gcaccacaag cttccccgct tcagttctgagggcttattc ccccagtggc cagatatgtt 15360 atctgctgac agctagctgt tgagtctttcttttggaatt gctcttggcc aaagggagat 15420 gcctcaccca aggtcatgac ctctccctggagacaacctg catccaatga gaagtcaatg 15480 caagagcaca aaagctcggc ccccttgcctcagcttggga caactcacag gagagctcca 15540 aactccagcg ctcctcagga tcagctgaggccattgttgc aatacatcat agttcatctt 15600 cttcctttgc ccagtccttt tcctcaccctctcaagggcg ttgagggcac tcctttaaaa 15660 aattcccaca cagagactct ctgtctctgagtctatttca ttggaacatg atccaaaaca 15720 taatatatat caaaatcctt gaaaacattcatccatttta acccagtact ctcacatcta 15780 agatagtcta aggtaatgag agattaaatcatagttttga gtttgctgtt gtaacattat 15840 aattttttaa aaactataaa gaatctaaatgttcaaaaag aaagaaatag ctaacataat 15900 ggtatagaat tctttacagc cattacagttcatatttttg agaaatatag tgacatagga 15960 aaatgttcac aatataattt taagtgaaaacagcaggata tagaactata aaattataaa 16020 tggtttgcaa acatattact taaagaactatcacctttaa gagaaatgca atgaccccag 16080 tggtaaatat atttaaaaaa atctaaggagatagtcaaat tttgtttaat tgagtaaaat 16140 ttgatctcat agccagacct atttttaggtaaaataaaga tagtaaattc tctagcactg 16200 gatatttgaa gaacagcaca aatgacacttggcttgctgt agagtagaat taagcatgaa 16260 tttaaaatgt ttaaggtacc attttgccctccggaattcc attctgcagt gagtgtctta 16320 gagatgtgtg gttttggtcg gcttgcctctgctttccctt ggctggcctg gctttggttg 16380 atttgtctta gttaacaact taactcttgaagacatctga cttctttggc taactttgac 16440 aattaggcaa gaagaaattt gtttgatcatctccacctca aaaccctact acctttagac 16500 ctttatcaca agcatagagg aaaagaattcaaagaaaaga aactcatgtg agatgttact 16560 accaagtatc tctaggcaat aggaaggagtaacccagacc caccattaag ggaaggggag 16620 tgcttttaga aaataagatt gtatattgagacaaactaat tgtgatgttg ttatgtcaca 16680 tgtttaaata atcccaggtg cacattctctcactttcatc agaacacaac cacaccaaag 16740 caggtcaaag aatctgactc ctttccaccaccaagcctct taacattttc tagacctgaa 16800 tacaaaccaa acactctcat tttcccaatttcttactccc actcaagata tttcaaacac 16860 gtgaaattga ttatttgaag gtttaagggagtgaaattta agattaaaga cattccttcc 16920 ccaaccactg ctccgcactc acatggcaacttggcctatg ctatagacag gggagatgac 16980 atttaagctc catgcagaga tgagccctgtctttgagttc tttgtggcct ttccttcagt 17040 gaagagtgtc agtccctgtc tgtccctgccctgccccttc taaccacaga tgtcctagcc 17100 tggccatgga tgtggagttc ctcagagcaaatagagtcca tgattttgtg ttctcagggg 17160 tcatcaaggt gtgctgatag ctttatcttcccttttcact tcatttttct ctaatatgtg 17220 attaaaatgc agggctatcc cagaaggagttgactagaga actaaaaaac aaacaaacaa 17280 aacaaaggga ccccaaaatg acttcatggttaaaagaaaa acataacagt gaaaaaataa 17340 aggcattata atgaactctt ctccatactctctagagccc tggggaaatc tctttgatgt 17400 agaatcactg aatgaagaaa agcccatgtgaaaccagaac acttgggttt atccaacaga 17460 tatttttaaa gcacttgcta tgtatcaggcactgtgctag atactgaaga tacatagacc 17520 tggacactgc cctcatggaa attatactctagcaactcag atttatcagt cccacaacac 17580 tgggtagacc gcacaacaat atcaggcacagtagtgccgt gagcttatcc atcccgtaca 17640 tgttcccagg agtgctaaga aagggaaatagcagccagaa acatcctatg aaaagattac 17700 ctggaacgac tcaagaaaat ctgcccaggtgcatctgttt acctctcctc ctctgccaag 17760 caggagaaca atattgaatt ggagaacacatatgttaata actccaaaga gaggctagta 17820 tctcagcttg agcaccagct ccctggatgctactatagtt ctgggcaaaa ctgcaaagca 17880 gccaaagttg gtcttgcaac catcccaactttagcccatt atcaatctcc cagacatcac 17940 atgcacatag cctaccctaa atctgccccaaatccttcaa tcagcctaga catgcctcta 18000 gactagtgtc tggtttaaaa tgtaaatgaggtttaacctt accagggatg cctcaacatc 18060 ccctggcaga ccttccaccc agacaggaagaggtggcata atggagcaag catgggtttg 18120 gggccagaaa gacctggttt tctaccccagtttcacaact tactattttg tgaccacaag 18180 caagttactt aacctcgatt aagcctcaatgttcttggcc acaaaatgca gataataatg 18240 tctgcccaaa tatatttcgg gaagattaaatacaaacaag ttcttcattt cttatcctat 18300 ccttcctcat ctcataaggg gaaccaataaagctcagcaa aaacacaaaa aagacattga 18360 ttttatttat ttataggcat tggcagaaaccccatcatga acagggaaac cctttggaat 18420 tctgctgcaa ttgattgcct tggctgggagatgtgaggtt cctggagcaa tgtgaagaaa 18480 tccaggggat cgcctgggca ggacccaggcctctaggcaa aatgtgtgga acaagccatc 18540 ggttttataa tcctcctaag aaagatcatgaaaagcaaag tgcattctta attatttcaa 18600 agagctctgg attctatata atctgtcctggaaataatga agagaagaaa gacaaaactt 18660 ggctttattt acagaggtga cgttacagggattcaccatc tattattacc tggcaatacc 18720 ttagggctgg cctccaccac tgctttttatgaagagtaaa gtacacgtga agtctgttgt 18780 tcaaggccct gacccacatc caacctggactcccaccact ctctctcagg cattctgggc 18840 taagctgtaa ctcagccgtg tcttccctacactagcgaca gtcttctgct tcatcctgcc 18900 cttctctcca cctaccaagc cctcctttcatgtcttccca tcaaccttct ccatatagcc 18960 aagcccctgc tcaaatggac cctctccatcagggagaggt aacagaagct agaacccagg 19020 ctcagggtcc atagaccagg ccccagggcctactggggcc agtcagtatg gtggtctcga 19080 taccacagac ctgggactca gtccataatgtggctatcaa ccagggtgcc cttagaatta 19140 actcaggagc ttttagaaac atataaatgctcatatcccc accccaaacc ttctaaatca 19200 atattccata ggggataaga aaggtatctgggtatctgaa ttttttaaag actccaccct 19260 ggattctaat gcacggcctc agctgaaaaccatggctcac agataggaac tcctctataa 19320 aaaggaaact agatactatg ccatgacttcatagactcta caaatagagc tgatggaaaa 19380 taatacaaaa aacagtaatt ggacaacctgaacatttaca aatcaaaggg gccaggtgat 19440 aagtagtttg gggtgttcaa agatctgagtaagaaaccag tgaaaagttt cattatttct 19500 cacaacttac tgggaagcag atgggtggaggggagtggaa actgaaaatg tctggacatt 19560 tgttctgaaa ctaaccagca cctgtaaatctgtaattagc aaagtaagga tagttgtggc 19620 taaaaatata gtttgtaaat gtatcaaggacagtggaatg tctcaagcaa taagcaaaac 19680 ggctttctta aggacaaaaa aatccaaaacaacatgatat cttgttttgg aaagggtaac 19740 catatgagaa gacaaaagaa acacagcagctgctgtgttt ggggcttgag ttgcagcatt 19800 tcatacaaca gctcatgaaa ttttaattgctgacatttaa aggttcaaat tggcagggag 19860 agcaagtcac atgggttgaa cactaacaaaagattacgaa ggaaaggtga ggctctgtgg 19920 cacatctcca gcaagaagag gtgcttagcagggtgccctg gggtcagtgc ttgctcactc 19980 cagattggat tctgcattct aattaacaatgtggaagaga gaacaaaaga cccctgaaag 20040 ataccagaag aagacttgca aagggggatgagggcaaccc ccagagagaa gagcaaaatg 20100 agacaggtga gcccacaagg cttaatctataggaaaaaac agcagacagc tgagactact 20160 tacaaaaagg caaactagct cattggaggaaaattcacta gaacccctta tattttagaa 20220 acaaaaggac acagaccaca aaaatgtctcaactatcaac atgggtaaga gtgtccagca 20280 ccctggagag gaggtcaggc tgtacaggaggcagccaacc caccttcaga tcaggatcag 20340 cagaggcatt gccaagacaa agtacattcagaatactaat taatatctat caatggtttc 20400 tctctagaaa aaaaaatgaa atgccacagggaatgtctat taaccaaata atccacttga 20460 agatcttttt gcccaactgc tgtcataatttggtttctct ttctaatttc aggttaagtt 20520 cacactactg tcataatttg atcagcatgtgcaaacagcc aaaaccagaa acacatccat 20580 acctctacag cagcctaatc taagaagaaaaaggagaggt tgggttggtg gtcaaaggga 20640 gaaaggaatt ttcattccct gtatctgcccctgggctaat aacagccacc atgagctgag 20700 tgcctggtaa gagtcaagcc ctgtatgaggcacatcattc gtattgctca ctttaactgc 20760 aatggcagag ttgtgaggca gttattagacttatgacttg gagaaactga gcaacttcca 20820 tacacatcta gcaaatgcaa ttgtctggatttgaacttgt gtccctcgga cttctcaatc 20880 tatgctcttt ccctgcattg ttcagtcagcccctgttctc tttaagaggg ttttctcccc 20940 agttccccca gctccccact gccctctattctgcctctgt gcctgagagg ccttgagagc 21000 cttgagagca tcactggaca gccctggcatacagaccctt tcactggggc ccggccaata 21060 agcaagcttt tcttttacac agaaatgtttgtggcagtct ccaccaatct cactgtccca 21120 ctagctccct gtggagtcag gaagggcaaagatccccttc ttctgggtgt gatcctactc 21180 cttggaaatg cttcacgtag aattctatggattccaactg ctggtgagtc aatctctgcc 21240 ccccctggat taagaatcag gtagaaggaattgaaaagga gctctgcagc cagcaagcat 21300 ttaaaaggcc atagagaaaa atgcaggaccgcttgagaag ggcagatagc agagcagcta 21360 ggagtgcccc tgctggagct agagtgtgagggtgtaaatc ctggccccag catgttctat 21420 gtgcacttgg gcaacctgcc caacctcgctcagcctttct tttcatatca gcaaaatagg 21480 ttgtagtgag caacagggta aagcacttagtaatgctcaa tggatgctag agaaacaaaa 21540 acggagcaga ggcaaagatt ccagaagcctcaggatgaac tttcggcatc cctagatgga 21600 gaactttcat atacttagtc tcccttgttcacttctccag ggactagtcc ttgcttctct 21660 tgtcttgtgt agactaatat acctctgaagttgttcttgc aaccatttcc cctaagtaga 21720 tgagggataa caaacaagca aaatatgtactgaagttggg tggctgcaag caatggactt 21780 ctttgagtgg agaaaaaaag ggaaggagatgtggggtggg gaagaaggag aaattatcat 21840 aaaattggat tccaaagcat tcagtatgaacattcccagc aatagcagat gctttttgcc 21900 tgctttagat aagacgtcta tgatttagacctctgcgcaa actgagaagc tgttggatgt 21960 tgaaacatta aatctaaaca ccgaatgcctgaacccaagc agaagaacaa gcccaaacaa 22020 gcctgcaggc cccgctaccc atcacatctcaccatgaatc aaaaacgcac tacttaggcc 22080 agctgccact gcagctgctg ccaaagcacaaggtgggtgg gacctgagca gtgttctacc 22140 atcaaggaaa gaactggatg agggtcctgcttactcggtg ggtgggttcc acgtggaagg 22200 gtttcctgcc acacacaggg tccagcatgagaaatggcaa aggcggggaa caattcaggg 22260 gtgcccaagg gagagaatac agtcacaggttcttagttgc tgtttctggt taggccagta 22320 aagccccttc ctcatccctc ttttccatttatcactagag acagaaacta aaagccatgg 22380 cttcaggctg ctaaaagcct aaaacaaaataaaaagaaca acaaaataag gcgggttgga 22440 cgtgccaact aaatgattgt atatactattttactatagc tactgccccc aaaggcacct 22500 ttggatgcca tctttggatt tctcacgatttttcaatatg tagaacatag tagattctca 22560 cacaatatct gcagaatgaa aaaataaaagtgtgcatgca aaggggtggc tgggtcactg 22620 tagtccaccc cagagaagac gggaaaatgcattttgctaa ggcacacagt gatgagaggc 22680 atcccacatg gtagaaagag aaccagactcggcatccagc ctctaaatga gtatgtacca 22740 gaatttccca aggctcttgt taaaaacgcagatcctggtc aggcgtggtg gctcatgcct 22800 gtaattccag cacttcggga ggcctaggcgggtggatcac ctgaggtcaa gagtttgaga 22860 ctagcctggc caacatggtg aaaccctgtctctactaaaa atataaaatc ggccaggcat 22920 ggtgacgtgt gcctataatc ccagccactagggaggctga ggcaggggaa tcacttgaac 22980 cctggaggcg gagcttgcag tgagccgagatggtaccact ggactccagc ctgggtaaca 23040 agagtgaaat tccatctcaa aaaaaaaaaaaaaacacaaa tcctaagtct tccctctcca 23100 ggaactgaaa ttctggagtg gggcccaggaatttgctttc ctaacaagct cctgggatga 23160 ttttgatcca ggtggcctgt ggttcatattttaaggaaca accattccaa gtctcactta 23220 atggtagcct taccttgagc ttcaaagatgaccagcatgc tggccacatt ttcccccaag 23280 ggggtctctt tagcctctta aatgctaatggataccagca gcctctaaga cctatgtgcc 23340 atgttatgtt ctggaggaga tgacaggcaaacccactcat caacaaaggg cttatttgta 23400 agagaagttc ctggtggggc cagtggtaagaaagcagggg tagcattatc tgaaccatag 23460 tccatgcccc actaaaattt ctgtcacaggaagtaagagc cttctgcgta aaagcatgtg 23520 tcccttggag agaacattcc tggaaaacatagaggttttt attgcttagt tttagaaagg 23580 ggacagggtc aatcccccca ttactatgaaatatgggtaa attgagagcg tagtatgctg 23640 caaagcatca taaaaataat tttacattgttactggcttg actttaatgg tgggttttta 23700 ggaggaaaaa aataataaga gaaatttttctgagtgctcc gacaggagag agaactctct 23760 tcagagttaa gagacatctg tcgcaaagctgactgatccc aagaggggtg aattcttgga 23820 agcccaagtc caagacgcac aaaactctgagttcaggaaa cagcccctct ctggagaagg 23880 agcctcacca ggtgtgtgag agtgaagaaaccaaacacaa gactaaggag gaactattaa 23940 tagcaagctt agctcccaac ctaaagccaatgaagtgaaa tgcaatctgc accctgtatc 24000 taggccaagg aaccaggaag acaatgctgtgccctcaccc gtgctggatg cagtctgtca 24060 gcctcaggct cagccctctg cagcaacctgatgccctgcc ttcttccaaa ccaagactgt 24120 cttctatttt gttctactag tgtttattgagtgcctactg tatactcttg gaagtgtgaa 24180 gaaatgatcc tacctgccta gcagtgtgtatacctgtcat tccagaccag tttccagtct 24240 catctgagat cataagacac ccataaagtcccctgcagaa tgttaaagaa caataaatga 24300 tgagaaattc acattacaag atctagaagcacaagatacg cataaatgag ttgtatccca 24360 aaactttctt tttaaattca tagtttaaaggtcagaaccc attttccaaa gggacagtgc 24420 tgtaaatagt caggtgtgta gtctaaacctaacaaattca tagtatagct aaaattatac 24480 acatgtgcca taaaaacaag gaaaacatttctgctacatt attataatta catttaaaat 24540 aactcaaaaa tatttttgag cagttatccttagtgatgac atccttggga aataatgttt 24600 aattagaatt taatgaaaga gagatttctgtagaaagtat gaggaaactg gactcataag 24660 gactttagat tcttatctca cattctttaaaaattttttc tctttgtaaa aatactacat 24720 ggttttgtgg aaattttggc aaatacattaaaattttaaa tacagatata aaaaataaaa 24780 tttaatatga aaccaaaaat taaaagctaatatgtatttc tgtccaagat tttttaattc 24840 taaatacata tgcatgtatt ttttacttgaaaaacaagtc acaaatattt ttgtaatcta 24900 ctttttcacc tttctattat caaattatctattttatcat agtcttctca acatcgtgta 24960 ttagctttct tcttaatcct tgccaaattgctcaattaaa aattatatct cattttaatt 25020 tttatttatt cgatcactgt tgaaattggtctttactctt ctccaattta atggccactt 25080 gtattttatc tatatgtaat tataattaattatcttttgc attttttacc tacttttctg 25140 ctggactgct tatagttttc ttgagctgaaaagattgtga atatataata atacctcctt 25200 ggttatttac atattgtaaa tattttcatggtttgtcatt taatttttat ctggttcatg 25260 gtgttaacat acagaaattt taagctttatacagtcaaat ctatccagtt tttccctttt 25320 agtttctccc tatgttttta aatattgaaactattttccc gggtgatcca agttcatttg 25380 cgcttatatg atgaaaaggt caaatctatatagatagaaa tacatcgaca ggccttgcct 25440 ctacccctac tgtctgcacc tcatttgtccccatcacctg tagggaacca attttatttg 25500 tgtctcactg atccttctag cacatctttttgcaaacttt aaaacaatta tctaattgat 25560 ctttctggaa taggtacatg gtattgcattttgaaaaggt tgttttttcc attaaatttt 25620 gtaattgttt acagttgata tcaaagaaaactgttgattt ctgaaccttt attttgccta 25680 cattcatctt gttgatctag taattttttagttgattgtc tcgggttttt agatggacaa 25740 ttatatcatt tttatgattc atattgataattttgtcttc tacctccaaa tagtatgttg 25800 ttattatagt tcttatttta ttccatctaaataagccatg attttacttt gtgatggctt 25860 tataattagc actatttatg gtttttaatcataattgtta tctatctttt ctagttctta 25920 aagggcatgg ccgtgccaca tctggagttgctgttatcat ttatgttaga taggatgggc 25980 taaactgtgt tgcttttagt aaaacaagcctaaaatctca agagcttggg acagcgaaaa 26040 cttatttatt acttaagcta caagttgatcatgggtcatg gtcaggtcag gtcatctaga 26100 tccaggccca ttccaccaga gcttatacaaccaaaggctg tcatttcggc cgggtgtggt 26160 ggctcacgcc tgtaatccca ccactctgggaggccaaggc ggttggatca tgaggtcagg 26220 agatcgagac catcctggct aacacagtgaaaccccgtct ctactaaaaa tacaaaaaaa 26280 aattagtcgg gcgtggtagc gggcacctgtagtcccagct acttgggagg ctgaggcagg 26340 agaatggcgt gaacctggga ggcggagcttgcagtgagcc gagatcaagc cactgcactc 26400 cagcctgggc gacagaggga gactccttctaaagaaaaaa aaaaaagaag aagaaagaaa 26460 aaggctgtca tttactgtga ctgaagctagaggaaggaaa gctggaaaat tttgcactgt 26520 cttttacagg tttctgtcca gaagtgacacacatcatctc caatcacatt ttactggcca 26580 aatcaagtca cttagccatg ttccaaaagggcatagaaat tcattcctac catttgccta 26640 aaagaacagc ttggaaatat gggtggacagagctaataag ttaccatatc actctaaagc 26700 acacacattt gtatacacac cacaaatgcacagaagagat tccttcacta ttggcgatag 26760 gttttcaata tgtcctagat ttttggaggagacagagtga tctaaactag ttgcacagat 26820 taactagatg attgttaaat gaactctaatccaataatac tcacctttta taaagattag 26880 agtcaactca aagataaaaa ttttttaaaagcatgagtta agagctttta cttaattctt 26940 ctcttcccct tggaaagcat aaattaactaaagtttacta tgattactag tggctgaatt 27000 ccatatttaa atttataatc atgtacagcatagctaataa tttaattata agtgtaaaca 27060 attcccaaga cgccaatagt tcattgtcaaatcttacctg cttcacaaaa tttgggtaat 27120 ctatggtcta gaaaagacac tgccttttataacattgaat aataacaaaa tggccatata 27180 ttcaataaat actaagtaat tactaaataattcagcctgg taaaccattt ttaattgtgt 27240 caaaagccaa aggtatcatt caatctttcctcatttgaga tgtattgcat ttcttatatg 27300 tgatggttaa ttttaggtat aaacctgactggattaatgg atactcaggt gcttggtaaa 27360 ataatatttc tgagtatgtc tgtgagggtgtttctgagag attagcattt gaatcagagg 27420 actgagtgag gaagattgcc ctcaccaagaagggtgagca tcatccaatc catcaagggc 27480 caaagtagaa taaaaaggca gagaaagggcgaatttgccc tcctggatct gggacatcca 27540 ccttctcctg cccgcagaca tcagaactccaggttctggg gctttaggac tcagggattt 27600 acaccagtgc ctcccacccc ccagattctcaagccgttaa cctcggactg agaattatac 27660 catcatctcc cccagttttc aggcctttagactcagactg aattatacct ttagcttccc 27720 tggttctcca gcttccacct ggaatatcatgagacttccc agcctccaga ataacatgag 27780 tcaaagctca tgtaccccgc tcttagagagaaagagaaat tctattggtt ctgcttctct 27840 ggagaaccct gtctaataca atatacaaggcaactcttta ctgtgaggaa aatggagtca 27900 ttcaatcctc accatcccaa acagaacaggctcttccacc acagcctcta tcaggccctt 27960 gtcggcaggc ctggggcatg aaatcacataaagtggactc tggatggaga aggcattcag 28020 gaaccagtgc accggtctgc agtgtgcaataacctcactc atgtgcccct atacctccat 28080 tttctacctc tctctaataa gaacatgagggattgctttt aatacacaga ttagcatttg 28140 ggccattcat ataaacatac aacaatcctgtcaacagtat tatagttggt gtcctatagt 28200 tgtgcctcct ctaacctgtg aattagcaatagatcatcct atccagaggc ttggtctgtg 28260 tgcttcttct aaaggtatca aaataaagaagttgtttctt cttttctctg ctccataatc 28320 aatatacaac acaaagcttc attcatgcatagtttgagct aggtcatagc atgaccatgt 28380 cctagaactt actttctgcc ttccgatatccatagtaaat agccaaaata tgtactcaat 28440 acatccattt ttaaaactag tcaatttaagataagaaata tcacaaatgg gtttttggca 28500 ttataagata aaaagatcaa ttgattgaaagtctcgttcc ttaagattga atttaattgg 28560 tttgattgac atcatttatc cacagtccataaaccaaagt tatccatcat cattgatgaa 28620 cggcatttga aaactatcat caaattagttgcttagaaat gccaagatgc caccttagac 28680 aaggaaggcc tctggcaaaa tatattcatgtgctgcctaa tgacttttgt ccaccatgga 28740 ctgcatatat gacagtagac tcataagattataatactgt attttattgt atctcctcta 28800 agattagata tgtttagcta cacaaatacttaccattgtg ttacaatttc ctacaatatt 28860 cagtacagta acatgctgta cttgtttgtagctcaggaac aataggctat accatatagc 28920 ctaggcatgt aatagcctat accatccaggtgtgtgtaag ttcactctat gatgttcaca 28980 caaagatgaa atcgcctaag gaaggacacaactctcagaa cgtatctgga ctgtaggaga 29040 acaacaatct gattatcaga tcttcaaaacgtgggcagcc ttacaagatt cagagtttaa 29100 ctgataagca tgtaatttgc aatttcctaaccctggaatt tgtcaagata atgttataat 29160 agtcccttat agaactaata gactcttccaataagtagat gctgctttgt gaattcagtc 29220 ctatcgtgag acttctgaat tgggatcttggaatctcatt agcctcagaa tcctaaattt 29280 ataataaaga tgcatagagg catagtgagtagcatttgga agatgctctt aattctcagt 29340 tctccagttt gtcttaatct ttttatataaactcctctaa tcctcaacct ggcttctgcc 29400 tgtgcaaaag tcaatatatg agttagttactactccccag tgaaagacga taacatttta 29460 ggagtgatac tttatgtata tttagcatttgtcatcccta aacttcagtt atagaaattc 29520 tcgcatgaat ccccaaaata tgtcagtgaactaacagtct gtaaacatac aatcatggca 29580 tttaatatcg tattagctac acagaaaaataaggtatttt atagcacatg ctcaagagat 29640 ttcactattg acattaaaat ctccaatggaaatactcctt ggaattgagg atatattgca 29700 gtagtggtaa aaaatattaa tatttgttgagaactcacca tagccctggc attctgctaa 29760 atattggata tccttttctc atttaaacctcactgctatc ctaggaagta gaaactatta 29820 ttacccctat tttaccagaa gcaaattgaatcaagagagt aactgtttta cccaaaatca 29880 tcagcaggaa tgttgccaaa caatggcctcactgcctaag gtacacagaa gtcagtacta 29940 tggcaccagc ttttgagaaa agaaagggctttattgcaag tcaactgcca aggagacagg 30000 agccaacact caaatctgtc ttcctgaacaagggtttgag agcaggtttt ccaggcaaac 30060 agcaacaatg caagggacag gaaaatgaaacaaggcatga tctgattgga tcatgcaaag 30120 aagtcacgcc aggtctttgg ttaagtccatacggcagcaa aacagggcac cccttgcttc 30180 ttaatttggc ccttgctcct tgatccaagcacttgggttc cacacatcat tgactttttt 30240 attccagctg gctccagggt cctgaatcaggtacccttag ttcatttggg catgctcagg 30300 taatgtgacc agcaacctgg gggtcccttgcactgaaaaa ctcatcattt tgttactgac 30360 aacgttgaat tggttctgcc attacagaatgacaaattaa caggaactgc agtgatctac 30420 atgaggtgtt ggtatcttgg gctaggtcataacaatggaa atagaaatga gtggatctgg 30480 ggtcactttg caggcaacag cagctgctgatagactgagt gtagcaggtg gagaagcagc 30540 agcaatcggg gtggctgcta ggattctgcttgagtagttg ccctgatggc atttatgtat 30600 atgagagaga agtaggttga tgcgtgttcccaagaggaga ataaaacaca ttgtagtcat 30660 gatctacaaa ttaaacatac aattatacacacagacacat gtatctatac atatttaaaa 30720 catgatccta gagaaggatg ttttttctcttgctgttttt catactgtcc tatcatggga 30780 gacccacaat taggactctg aagacagctgtttgcttggg gttttgctaa tgcttcggtg 30840 tatcaaagta aggcacacac tcctgaggagaaatctggca tggaaacatc cttaaatgcc 30900 accctacccc tcatggttta tataagccttagctacagca ttgtctagaa atggctattc 30960 tttgtctttt ctttggaaca catttctcacttcaaatgca actacccttt gccagcccag 31020 gattttccca atactgaacg ggtgcccatcttcaaggaca attccaaatg tgtgcccctg 31080 cctttggaac aatgaagagg gtttcgctcattgtttttct tgtcttatct gcatccagaa 31140 atttctggct tgtgccattt taattttatatacttttact ttatttcatt tgaattttag 31200 aaaataatga gtatttaaga gaataagtgtttgaatttaa acaactagga gtaggattac 31260 tgcatgtaga gcctgggatt gccatttgaagtcattaacc agcctgagac caacctttcc 31320 agcatgcctg cagggatgtc ttcccgggttctgatacatc gcacattgtg tgactcccaa 31380 gcaagctctt ggccactcaa agaaaaaccactgaatcaaa ggagatggca tatgagtcat 31440 ttcatcaatt acttatttca catagatttaaaactatgtc ttgctgagct caacttgact 31500 gcatgtttga atagtgaaag acattgtgtgaaaaagaaaa ggggcaaatc taatcacagg 31560 cacaaatacc cttctagaac cacattttagataagagtgt cccgcagagg ccgggtgcgg 31620 tggcttacgc ctgtaatccc agcactttgggaggccaagg cagccggatc acgaggtcag 31680 gagatcgaga ccatcctggc taacatggtgaaaccccgtc tctactaaaa aatacaaaaa 31740 attagcaggg tgtggtggcg ggcgcctgtagtcccagcta ctagggaggc taaggcagga 31800 gaatggagtg aacccaggag gttgcagtgagctgagatcg cgccactgca ctccagcctg 31860 ggtgacagag tgagactccg tctcaaaaaataaataaata aataaataag agtgtcccgc 31920 agaacaacag ttccttggga tattaacaggtattctctct tttttttgca aattttattt 31980 cttttaaaaa attattgtag gcacatagtaggtgtatata tttatggagt acatgagatg 32040 ttttgaaaca ggcatgcaat gtaaaataagcataccatgg caactgggtt atccgtcccc 32100 tcaagcgttt atccgttgag tgcaaacaattcagttacac tccatacatt attttaaaat 32160 gtacagttaa gttattgact atattcacccgttgtcttat tcattctttc tattttgtgt 32220 acccattaac catttccacc cattcccagccccccactta acagcgtact ccaaaagggg 32280 aaaaatgatc tgtggtcacg tgagtaatgctaggttaagt aaatttgaac aggtttcttt 32340 actatagact tctctagatc tttttgttgttgatgctttt ttgttttgtt ttgttttgtt 32400 ttgtttgaga tggagtcttg ctctgttacccaggctggag tgcagtggtg caatctcagc 32460 tcactgcaac ctccacctcc caggttcaagtgattctcct gcctcagcct ctcaagtagt 32520 agttgagatt acaggtccgc accatcatgcctggctaatt tttgtatttt tagtagagat 32580 ggggtttcac catgttggcc aagctgttctcaaactcctc aggtgatcca cctgcctcag 32640 cctcccaaag tgctaggatt acaggcatgagctaccgcgc ccggccgact tctccagatc 32700 tttaatgtga tgctaatagg ttctgtatctccaagaaagg agagagggca tttaactgtt 32760 tcccaaactt atttaatcac agaaagctatttttgagaat gttttgtggc acatattttg 32820 agaatctcaa ttccaaagga aagaaaaacataaataaggg cttaattgaa aggacaacct 32880 catttaaaag ttgacataga ggccattatttgggtcacag aaaactaagg cctgagattt 32940 gtcatggagt tcatgtcccc atttggtcactaagatagag acttgtcatt gtaatggctg 33000 ctcagcactg gtgaacaccc gccatgaactgacccaaacc agtgtggcca cacatgaggc 33060 gaggcatgcc cgttatcaca ctaccaaatacggaaaccaa gtcttaaacc aattttttgc 33120 tccatataca gacatttact ttacaattgcttattaacaa ttcatacaac acgtttggtt 33180 agctcaaaga agtcccattt tctgtccttttaggggctct cagcaccacc ttgactgaaa 33240 cattttctca aggcagacat ggcaacatgcccgaggcagg attctggaag gttcctaggc 33300 catgccgggc aacttggaat tctccaactaagtgggtgga cactgggcca gttagcgatc 33360 aagtctcagg cactatccct aagaaaaaggctgcagtctt tgctggagcc ttataccctc 33420 ttcattaaaa acaggaaggg ctctaatattttagccaatc gaggtagcag tattagtttg 33480 tgagggacca caggctggta acaatagaaatttattttcc cagagtgctg gaggctggaa 33540 tgctaagatc caggtgttgg caggcttggtttctcctgag gtctctcccc ttgttttgca 33600 gatggtcatc ttctcactga gtcctcacatggccttttct ctgtacatgc acatccctgg 33660 tgtccctgag tgtccaaatc ccctcatcttataaggactt cagtcagatt ggattagggc 33720 cacaataatg gcctcatttt aatgtaatcacctcttcaaa aatcctatct ttaaatatag 33780 tcacattctg aggtactggc gcttagggcttaaacatatg aattggtagc agggacacaa 33840 ttcagcccat accagtatcc taaatattgttttttttatt ataatgatat cccatatcta 33900 atgttcattt tgctaattaa tgtcactagtttccacagtg aaaactcctt tcggatattc 33960 actatgctaa ttagcaaagt ctcatacacttctcttcttt tttaatataa tttcaacttt 34020 tcttttagat gtgggggttc acgtgcaggtttgttacctg ggtatattgt gtgacgctga 34080 ggtttgaggt atgaatgaat ccatcactgagatagggagc atagtaccca ataggtcgtt 34140 tttcagtccc ttactcccct ccttctcttcccccgctagt agtacccagt gcctattgtt 34200 gccatctcta tgtccatgtg taccaaatatttagccccca cttataagtg agaatatgtg 34260 gtatatggtt ttctgttcct gcattaattcactcagaata tcagcctcca gctgcattca 34320 tgttgctgca aaggacatga ttttgttccttttcatggtt gcatagtatt ctatggtata 34380 tgtgtaccac attttcttta tccaatccaccattgatggg cacctagatt ggttccatgt 34440 ctttgctatt gtctcactca cttccttacatcactagctc cataatcagt caggtgcaag 34500 ggcatctgat tgactgagcc taatagcaagggaggctggt gaagtatgtt tttggctttt 34560 atcctgggag gaaaggactc atataattgggctctttttt gcagtatctg tatttctttg 34620 catatttcag catagacaat gtgatatcatctgtatgcca gcttcaatgc taagggcagc 34680 caattacctt aatagagaat gtacactggagggttgctta cgtagcaaca ctcaggagac 34740 ttcaacaaat gctccgttta tacctataaactgagtagtg tcctcatagg atcagcgttg 34800 gatggggttt ggccaaatgt cattatttcaactggttctc tgtcttttgg agattctgtg 34860 ttacctcaca gataattctt gtaataaatcccttttctgc ttaaactagc cagaattcat 34920 ctttgcagcc aagacacctg atacagttaccaatccttag attttcagaa agcatatgca 34980 agatatgtta tggtatagtg atctcctctctcataaaacc agggaatcac aggttgacca 35040 gctgacctgc ctaatttaga catctctatacctatagcag ggagaattag agagtcatat 35100 catgttggaa ctagaaggga ctgtagcaatcatctaacac agatgaagaa atagtcctag 35160 agagaaaaga ttgctccagg ccacatccatttttatccct acccccaaaa ggatgtttta 35220 aaataacccc tgtaacaatc ctcagcctggagagaacaat tttccatttc tgtttaaacc 35280 catatcccaa ggtggcggag acaacatcaggaaaaagctg cacagccagg tcttcattaa 35340 agacacgctg gagcccaggt gggcttgcagagtgacctat cccccagact cggcagtgag 35400 tcactacagg gtcagttcag catgtttttctctgtttcag actacagttc agctaggctt 35460 taggtcggca tctttttagg ttgctagggaggcctctctg gtaggggtat ggagaggaaa 35520 acaagatctc ccagcctaac cagaccaggctagacaggag agaggcgctg ctgtttggtt 35580 gtgggtgtca tccagggtaa ttcacttttctgtgtttgcc acccagtcat atctatggtt 35640 gtaggtttgt tgttgttatt gttgttttcctttgagtttt ttttgtgtgt taaaacctaa 35700 actgttcaaa gaaaatgcat gctgttctcatccctcaacc aagcatgttt tctcaattag 35760 acgcattttc acagagaatt agtcttaaaacccctgtgag cttaaaaata gattaagaac 35820 tatttcttaa gctccctttc tttggaatttaaagtgtttt tgagtggagt ttttttgggg 35880 ggtggaggag aggaagttta gcgtaattaagtagatcaag aaaaaaaaga aaatcattta 35940 actatttctt atatgatcct caggctttgaggttcactca tgtaaataaa gctcatcatg 36000 gatattaatt cattcattcc tatactccttcattcacaac agactgtgta tctatcaggt 36060 tcaaggcact gtgctaaaag aaatatacaggaagaagccc ggagcattta aaagcaggat 36120 gttgacaaac acacacattg aagatactatgatttgagct tctgacatct ggactaatta 36180 aagctgagga aattggcatc tatttggcaaaatggccaac aaagaagaca ccaggcagaa 36240 caacccacag aaaatttcat cagccaggaaattagaattc actctcccca tccaaagaga 36300 aagttaatta ctggctttgg tggattgcattcctggctct gcactattat cttaacacag 36360 aaggcaagag aaggcaagaa ggggctgaaagagccatcct tccaaaatat acttgggaga 36420 attaaaacac cgatgttctt ctctggggcccttgtgactg gtcagatcct cttgcccaga 36480 tcataagagg gcttagtgga aacaaaaatggtggttgtga atgtgaacac tattaaatgg 36540 tctttcttct gtatccaaag gtctaagcctttctgaatgg agtctttcaa gactcagctc 36600 agacactacc ttttcaagga aaccttttgtgacctctctg ccctcaaccc cacacacaca 36660 accatgccag ctagaccata gatcttccttagcatttcca cacatctgca ttacccctca 36720 tggcccagcc tcattatcag catccctcatgaggctggtg gacacttggg gtgtctctgg 36780 cacattgtaa acccttcata aatgatttctgaaggactgt aatacatagt ttatacaatt 36840 ttcagcatac gaatttaagg gaaagcataaaagaaaaata cccatggtgt acatttaaat 36900 aataataata cataaaggaa atttcacatatttaaacaga caaagactga cttatactag 36960 aagagaaata atgacctatc ccacagggtatatgtaacac catacgtttt ccaaactgct 37020 aaaagagagg tagtttgcct aaagttaactcccaactcta ctcacttaaa ggtggagggt 37080 ccatttattc ttaacagtga ggagcatttcagaatagaat gttctcacac acacacacac 37140 tgaaggcaca taatttgagc tactgaaaccttgaactcgt taaagcttag ttgttggaat 37200 gcatactcct aactttgcaa gattattacacttagagaac atctgagatt gcagttaaat 37260 aaaacttagg tttgtctgaa aaattaattagtgtacataa ttattctgcc acataaagca 37320 aggtatcaga tgtaagcaat tcatgtatgttattttaata attagcacca ctaagttatg 37380 ttccttttcc acctctggct taatttattctctaatatgt gtacaagtgt ctggtctact 37440 gtgttcccca caccattaat gccaaccacacacacgtaag cacaggcagc aaaagattaa 37500 aatcctgcgc accctgtttc gcctgcctcttcacactcct gcctctacta tggccccggg 37560 tggtgctccc cagtactccc aagctctctgcctcctcatt ctctctcttt tcccctactt 37620 ccaggggagg attcacatcg gatgggactgaaacgtatac aatttagggt ttcttatttt 37680 caaaaaataa tattaaatta ttaattctactgggtgcggt ggctcacgcc tgtaatccca 37740 gcactttggg aggccgaggc aggcggatcataaggtcgga agttctagat cagcctgacc 37800 aacatggtga aaccctgtct ctactaaaaatacaaaaaat tagccaagta tggtggcaca 37860 cacctgtaat cccagctact caggcaggagaattccttga acccgggagg cggaggttat 37920 agtgagctga gattgcacca ctgcactccagcctgggaga caaagcgaga ctccatctca 37980 aaaaaaattg ttttaaaatt aggtagaaataaatatttat ttaaaaagag aagtttcatc 38040 aaactaaaac acaaagtaac aaatatcacaaaatctaagg gaaaaaaatc tcaaaataaa 38100 tgtattagat agttctttgt gtagcactggtccacggact gccttctgac tatgccatgt 38160 atatttcaaa ccttgtctct cctgtatgacccacatgcat ccaggggaag ccactgtagg 38220 ttgacatgac catcatgata agacctctagcactacatct tcaggtcagg acagcaggtg 38280 ggcgatagga agatgaatgg aagccattccaacacaagga gggctagccg ttacctcaca 38340 cagtgactgc aaaccacaca gatatatcttcacaacctgt cccctgagcc agatccaaaa 38400 attgcctgta gtcactctaa ctctctaacagagaagtgtg actgaggcaa gttaaaatgg 38460 aaaagtcaga ggatgtaagc agttgtagctaacatgtctt acttgtgcaa atgttgccaa 38520 acatgaccac atgaacgcat cactagggcctctctagggc ctgggaagga acccaggcat 38580 gtgagggacc ctgacaccta agcctctacagcttcttggt aatcccacct ctgccttcct 38640 cccacacatc ttcccatgga ttccagtccccaagcttctg ggtccagcaa ggcaatgagg 38700 ctcaaggcac tgagcgccat gccttagggcaggatggaca aactggcaaa ggaaacagga 38760 agagtaaatc acacagatgc cctctgccttcaagtcttgg gacaaagagg gatggaggga 38820 gggattagag ttctgagcaa agacaggactttgaactcaa gcaagtggac actagctcat 38880 tgaataacca tgagctaagt gcttcacacggattactgct ttcatcctca ctaccaccct 38940 aaggaaaagg agagagggaa ggagaagagaaagaaaaaga ggaaggagaa aggtaagaga 39000 aaggatgatg atgaaaagga ggaggaagaaggagaacatg gagaagaaga atgtaaaaaa 39060 gaagaaggaa gacagaaaga agaggggtaggaggagggga ataagaaggg ggaggaagag 39120 acacagagga agaagagaaa gaagagaaagagatcaagtg aaagagaaaa agggaagggg 39180 agggaaaaga gcaaagaaga aaacgtaagctgatagagag agataaaaga aaaaagagaa 39240 agtttactcg tgaactcctc ttccctaggaagcagcagtg tttatctttg ctgtcataat 39300 aaaattaaaa gctttttttc agactatacactcatcctct taaattagaa agagtgacaa 39360 tttcttttgg ctcagttctg caaaatgcaagttgtcatga tctcacctct taaaaaaagg 39420 aaaaagggaa aaaagcaacc tcccaagagatggctatcac ttcaagcgaa tgcccagcgg 39480 gccagccatt tggttgctgt ccatgttttccagtggcatg tgagcctttc acccagacca 39540 caatcccatg ccaagctcca tgtcatgttcaatcttcaaa atcttttgta caattccaac 39600 actttccagc aagagttgat tttctttatgtttgcttgtc ttttaactga ctgacactca 39660 taattttctt ccaaggaaaa aagaatagcagaaaacttga aaatatcacc taatctgtac 39720 tgcacatgtt aagactgttc agaaaagttgaaaaattata attcgcaaat gggtggcttg 39780 aagtaccagt tgaacctcag aaaaattaaattctttagga ggaatgcctt taattagaat 39840 cagaagtaga ctctgtcatg gaagtcctggaaactccatt atttcacaga tttgcagact 39900 catagacttt cttatttatt gtcagaaacttctggaagtc tctcaatgaa tctccaagtt 39960 actgggtgag accacaccta gcccaggattccagatgaac tgcctagtcc atattattgg 40020 tgaagacacc caggaaatat ttcacaacctatctcagtaa cattccaatg tgttacaatc 40080 ctcgctttta ggaaatcctt cattatttccaaagatactg ctactgctct acatcctttg 40140 tgaaaataac aagatcaaaa cctttataatctcaaaaaaa aaaaaaaaaa aaaaaacagg 40200 aaaagaatta aggaacattc tctcttcttttttttttttt tttttttttt tggaaatgga 40260 gtcccatcct gtagcccggg ccagagtgcaatggcgtgag ctcagctctc tgcagcctcc 40320 gccttccggg ttcaagcgat tctcatactcagcctcatga gtagctggga ttacaggcac 40380 ccgccaccat gcctggctag tttttgtatttttagcagag acagggtttc accatgttgg 40440 ccagcctagt ctcaaactcc tgacttcaagtgatccacct gccttggcct tccaaagtgc 40500 tgggattaca ggtgtgagcc actgcgcccagcccacattc tcttcttctg caaactaaca 40560 tgtctatacc tttttcagtc ctttaatgacctgtgtggac cctgtacata acaacgtgct 40620 ctgtgggccc ctccgttgtg ggtcgaacaattaaacacct cactctggtt ggtgccagcc 40680 agatgagttg atgaaacctg agaggccagattgcttccag ttctgtaaaa cctgtttgag 40740 aattaaaaga gatgctccat catgtcctaggctgagtaaa tatgaaggaa cagtgtaatt 40800 ctatggcaac tgacaatccc aacagtaagccaacacagca ccacccccag gagcaacagc 40860 aagtgtttga gtgggttcaa aagagagttggggaactagg gggattgggg ctgcacgtga 40920 cgaggagaaa gggtgtggga taacatgggttggccttggt tgataggcta tgtccagtct 40980 ctgctgcctc tgtctctcct tcctcctccttacctcactc ttgagccccc cacctttctc 41040 tgtctcccta tctgaaacac ataccctgctcttaattggg aaactagtaa acatttctgc 41100 tgattttaat cctgaaacat cattgagaccatatgaaatg agtcttaagg atcagaacca 41160 tatgaaggga aggactgtgg gctggcagccggcgcacctg tgctgtcttc tggacttcca 41220 ccaccaacag tctccaggac ctgtttcaagttgcttcacc tctttgaacc gacttcatct 41280 attaactgag tggttgaact agatggctctttctggctta aaaattctgt gatttcattt 41340 ctggaggaat ttcaagcatg ggtagagagagaagaaactg agaggcaaga ggtatggttt 41400 ggactcatca aattaaatca caggtgactttccagtcccc ccaccacccc gatctgtaag 41460 gctgcctttg ggtgcgtggc cttcctgcaaaatggcaaac acttgaaaaa gcttccacgc 41520 tgaaacccaa accagaccat atttaggatgccccataagg tggcattttt tactaagatg 41580 ataaaggata aacattggtg gagccagagaggctgctccc aaatacagtc ctccccatca 41640 gcaacactgc agtagcctgc acctatcgtccagagtatga aaaagacttt tgccaaagac 41700 ggcttttggg gatttatcat tattacatgttaagagcctg ttctccatgt cctcaccttc 41760 ctaccttgct aatcttcccc atttccatctttcggcccag tgtgagacct caccatctcc 41820 ttttcccatc ttctttcctc catacccttggtttcctagc aggtcaatgc ccctgcccct 41880 tcactggaat ccatccatcc tggttattcttttctttatg aacctgctgg actacatctc 41940 aaagttaccc aaaagaggga tacaaagacacaataaaatc ggaaataacg ctcaccctta 42000 ccagcctgca gttaagagtg tgtggaactagggtggctgc aggcaaagag atagggggaa 42060 gggctgcctc atgccatggg caggacagtaaatggtgcaa attttctgga aagcatctta 42120 gtaatatagg tgaagagcct ttacaatgtgtttatcctat gacctatcct tacttctctt 42180 attctaaagg aataagaaga ggtttaatcaaagcatgagt gaaaaggcca tccaaacaca 42240 gggaaagggg ccgttatgat gtctacggttctaagaaata gaaaacaaca acttggctta 42300 aataataagc acataaccaa agaaatctagaagtaggatt cccttaagag ttgagagatt 42360 tagcagctca aaaatgtgac ctgacatacgagttctttct atgcttttgt tcttcctcct 42420 ccaacactgg tttcacacta aacttggctccccagtggcc aatagcacca aacagcactt 42480 atcggcattc ttgttggtat ccaaaagagatggagaaagc ctgatttcac atggctcttc 42540 cttaaaagca acaaaggact tttcccggaagccttcagca aacctcgtca gatttgaatc 42600 acatgccaat tcctaaatca gtcactggcagggaagtggg aaaaagggga ggaggtgttt 42660 acccttaacc aatatgccta cccctgcccactgagatcaa ttccataaaa gtattgcttc 42720 agttctttga agaggagtac aatgtttgtgagtcactcac cacatttacc caccaagagc 42780 taagggataa ctgagtaaaa tggtagagctggtaggaaaa tggtgagtag tcccgtcctt 42840 ctgtgccata attgacttgt acacaaagagatttggaaat ttaaacataa gaactctctc 42900 ttcccaagcc cagggctttg ggcacttcaagtctggtcct tgcctgggca cagtggctca 42960 cgcctgtaat ctcagcaatt tgggaagccaaggagggtgg atcacctgag gtgatgagtt 43020 tgagaccagc ctggccaaca tggcgaaaccccaactctac taaaaaatac aaaaattagt 43080 tggatgtagt ggcacacgcc tggaatcccagctactcagg aggctgaggc aggaaaatcg 43140 cttgaatctg ggaggcaaaa gttgcagtgagctgagattg taccactgca ctccagcctg 43200 ggagacagag caagactcca tcccaccccccaccaaaaaa aaattctggt ctttacatgt 43260 gaaaaaataa aagctaattt ttaaaaactaaataataaag agtgttaggt gataagtttt 43320 atgatagaga taatgtgtgc tttaaaacacacagcataaa acagatggaa aacacctcat 43380 ggagaaggga gagcttgaat tggtgtttgattgaaggaca agaaaaatct gttggtaact 43440 tcctcctggc tctgacctct gatgcagttaaagtaaagca acttagaatt atgatctagg 43500 gaacatattc agagagaaat gaatttatgatgtcttcaat ttgaagatgc tatgatttta 43560 gactattcta gaatacaatg atttttcattgcttcacact tgtaagaaat gttccactac 43620 tgaaaatttg caacacttaa aaaattcctacacaaaatca cagcccacct tgctgttcta 43680 tgctaaataa acaaaatatt ttttctcaattatgttgctc tccttaatta cacgcaattt 43740 taaaactagc tgtaactgac agccctttcctcacacttac tgccaatgta tttaggtcaa 43800 tcccacctcc aattagagaa acttagaaaaaggaaatcct acggtgaaat gccatatgcc 43860 ttacaatttt cagtaactaa tcctcaaggatgacaacttg gcaaaaaaga gaactggacc 43920 agctccctga aaggtccatc acttcggcccatgttaattg attgccttaa aatcacagtg 43980 cttccaggtt gtagagacac actcaaaatggactattgcc ctcacattaa aggggatagg 44040 ttttcttttc ccttttccca tccctgcttctctcaaagct gcacagaggt atatgaggtt 44100 tggggaatat ttgcagcaca tgttggctggcaagaatgtg ggtgaattcc tgttaagggg 44160 cgcagacact ccctcttcta tgacctttcctttccaccca gaacaactga agtatcatga 44220 taagaggaag ggctttgtgt ttgagataagcagctcctat acctgtcagc tttctctaat 44280 aagaaagctc tttcctgcca ccgtataggtcaccttctta ttggtaaagc atgatgaagt 44340 ttggaaactg ttgtccttgt tttgttgaaagaagccaagt tccaagaaat ctgcagaggc 44400 attcctacaa tccctcagcc atggcagcatgaatcctgaa ggggcagtga gtgctggttt 44460 ctactctgtc tgcacagcta agcctaggggagataatctt ttcatacaag atgcattctg 44520 cttttgtggg cctcttgcag ccctcaagcccccatctgat ttgtacacaa tgatccagtg 44580 ggccagagga gcccagagcc atgagcggcccatccctcca agaactattt ctgactgtcc 44640 agtatcatgg agcaagtgga aagaagaaaaaaaaaaccca attacttttc gaagagcaag 44700 atgaatgctg tagaaggaga aggaaggggagggagatgga tgggtgccga ttccagaatc 44760 ttcagatctg cttggatgaa tcattacctatgatttgcgg gacaagaatc tgattttatt 44820 catcaaccag tagaaacttt tctttctgcctcccaacatc tgaaatccaa caaacatgtg 44880 ccttaggaac ataccggtca tcttttagaggcattttata tacatattga gtaactagaa 44940 aacactcttt ccgtaataca cacacacacacacacacaca cacaccatct tgtcatacaa 45000 cactcccacg caagaaaagc gaaactgctgtttgatgaat gtaaacactt ggctgtttgc 45060 agcagtcggg agtcctgcca ggtttaagtgctaagatggg aggtgaaccc caggggtttc 45120 cccctgcccg tgctgagatc cttatttggtcaagcttcta cctatgccct ggcctcggag 45180 cgagcccgat agcgctggat cacagcagagggagcgaggc ggctgacgtc ccatcccgaa 45240 gagatgaatg gaattccagg aagctagagtcatgctggct tgggacagtg gcttggagac 45300 cagacttcaa tgacagaagc actaggcagcggcactcatg gcaatgtgtg cacccacaga 45360 aatgtaaccc acacctcggg ttcaggagccgaaaaatgaa aagaacgttt agggaggaaa 45420 aagggaaata caataatagg cagagagtaatttattactc tatgggtctg ctctgtaaat 45480 agctgaagac tctggagcca gatggttctgcaaattctcc aaacaggagt cacgttaaga 45540 agcacgagtg ggcacaaaaa ctgtttttcaagacacaatt tcaatttggc ttgtggaaac 45600 tggatacgag taagtttcct taaaattcgagtagaaagca gctgtcctcc ccgggcccct 45660 tgatgagaat acgcacaccg cccccaagcggccggccgag ggagcgccgc ggcagcggga 45720 gaggcgtctc tgtgggcccc ctggcagccgcggcaggaaa gggcccgaag gcagcgaagg 45780 cgaacgcggc gcaccaacct gccggccccgccgacgccgc gctcacctcc ctccggggcg 45840 ggcgtggggc cagctcagga caggcgctcgggggacgcgt gtcctcaccc cacggggacg 45900 gtggaggaga gtcagcgagg gcccgaggggcaggtacttt aacgaatggc tctcttggtg 45960 tcccctgcgc cccgtcggcc catttttctttttacaaaac gggcccagtc tctagtatcc 46020 acctctcgcc atcaaccagg cattccgggagatcagctcg cccgaaagcc cctgcgccac 46080 cccgcgggcc ctcctaggtg gtctccccagccccgtccct tttcgggatg cttgctgatc 46140 accccgagcc cgcgtggcgc aagagtacgagcgccgagcc cgtgcgcgcc aaggctgcgt 46200 gggcgggcac cgacttttct gagaagttctagtgctccca agccccgacc cccgccccct 46260 tcactttcta gctggaaagt tgcgcgccaggcagcggggg gcggagagag gagcccagac 46320 tggcccccac ctcccgcttc ctgcccggccgccgcccatt ggccggagga atccccagga 46380 atgcgagcgc ccctttaaaa gcgcgcggctcctccgcctt gccagccgct gcgcccgagc 46440 tggcctgcga gttcagggct cctgtcgctctccaggagca acctctactc cggacgcaca 46500 ggcattcccc gcgcccctcc agccctcgccgccctcgcca ccgctcccgg ccgccgcgct 46560 ccggtacaca caggtaagtc gcccccggcggccgccgagg accaaagctg cccgggacat 46620 ccacctggag cgctgaggct tcagtccctctggtggaccc cggaacctac actctccccg 46680 ctcgcctacc ccagcccgct cctctcagccgctggaggac tcttcagggc aaggctccag 46740 agccatcctc tccagccttg aggttcacaaaccaactcat caggacaccc caagatttcc 46800 ttactctctg aagtcctcct taagcctttgtatcagcact ccagggaaga gtctgtactt 46860 cccctgccct ccctgcaacc ccaaactacagttcctgatc ttgctcacct tcgacttccc 46920 aaaagccccc aaattgttgg tcttgcgccccccacacttt aaaaccagca tctctttcct 46980 ccacctctct ctctctctct ctctctctctctcatgctct ctggaaagta atcctgttgt 47040 ccttcatttc agcaaacgcg gggtcaaagagggatggtcc cggccctgtc cccatgccca 47100 gccccgtttc tgcgcaccgt ccctccctgcctagctgatc tctgaccctc ggctcttgtg 47160 cttcctgcta caggatccct gctgggcaccaacagctcca ccatggggct ggcctgggga 47220 ctaggcgtcc tgttcctgat gcatgtgtgtggcaccaacc gcattccagg tgagtttgtg 47280 tggcaccttt aggggaagga gggacaaggaagaggtgctg gctatcccag gtgtgccccc 47340 tcacgtgctg tcctctccct cttgagcctgactggacatc aggacgcagc ttcactctga 47400 tcctggtatt tattcacctc tttcagtggttgccaggagt tttcacccca accctttgtc 47460 tccaccccta aggactcagc cccctactgctggtcccagc ctagaaagct cactttgtgt 47520 tctctcctgt ctaacagagt ctggcggagacaacagcgtg tttgacatct ttgaactcac 47580 cggggccgcc cgcaaggggt ctgggcgccgactggtgaag ggccccgacc cttccagccc 47640 agctttccgc atcgaggatg ccaacctgatcccccctgtg cctgatgaca agttccaaga 47700 cctggtggat gctgtgcggg cagaaaagggtttcctcctt ctggcatccc tgaggcagat 47760 gaagaagacc cggggcacgc tgctggccctggagcggaaa gaccactctg gccaggtctt 47820 cagcgtggtg tccaatggca aggcgggcaccctggacctc agcctgaccg tccaaggaaa 47880 gcagcacgtg gtgtctgtgg aagaagctctcctggcaacc ggccagtgga agagcatcac 47940 cctgtttgtg caggaagaca gggcccagctgtacatcgac tgtgaaaaga tggagaatgc 48000 tgagttggac gtccccatcc aaagcgtcttcaccagagac ctggccagca tcgccagact 48060 ccgcatcgca aaggggggcg tcaatgacaatttccaggtg aggcttcttc tctgagccct 48120 gctccgtggg atcatctgct agacaggtgacctgccagga gggctacagg aaatcctgtc 48180 tgtaaactaa cgcagcagtt ctcagattttaggcagcatg agcatcacct ggagggcttg 48240 tgaaagctca ccctgctgca ccccagccccagagtctggg attctgtaaa actgcaactc 48300 taacaagtac caggtgatgc tgatgccggtggtctgggaa gcacactttg aaaactactc 48360 aaggaaacaa gaaatggttt tcctttagcagtcagcctgt tcaagcctta gatactcctt 48420 aggctcttaa gaaaagagct tatttcaaatgcattccccc aattttaatc ttgtttgaaa 48480 tggatacttt ctatgggcag aatcagtaccactttgctat tggattcctc atggggatgc 48540 agctggtctc acataaaagg ttaaaatagcagttagttgt tgctgttaca gcttcatctg 48600 tgccttaact tctagatctt tgcaacggtggctttgaact cccacattta actctttgac 48660 actggaaagt gtcattatat cctctaggcttaggatcaga ttccatttcc tgtgtaccca 48720 agtaggctga taactgaatt ctttgtaaaatactcaagac ttggcagaag tcacaattaa 48780 gattttgaag ttgtttggcg attaatgttgctgtgttatt ttgacatagt gatcttaaga 48840 cagctctttg aagcttttag aacatctattgtatgctttt cctagcatac agacagtctc 48900 ccccaacccc atccccaccc cgctctgcattctacaagta atgtgtgtcc tctgcccaca 48960 gggggtgctg cagaatgtga ggtttgtctttggaaccaca ccagaagaca tcctcaggaa 49020 caaaggctgc tccagctgtg agtacccctctatttttagg gcacataggg aatcaggggg 49080 aattccacca aaaacaaact gaggaatttagcaatagtgg ttatacataa attaccccca 49140 gtgagatcat caagaggcat acagaagtgactccaagggg tattatacac acacacgtat 49200 acacacgtgc gcatacacgc aacccctctacctgcatcct tcacaccaaa tgaaacgtct 49260 tctaccacta agaactcaag aggctggttggtaagagtat ctatttgaag ttttcatttt 49320 gtttcttgcc agctaccagt gtcctcctcacccttgacaa caacgtggtg aatggttcca 49380 gccctgccat ccgcactaac tacattggccacaagacaaa ggacttgcaa gccatctgcg 49440 gcatctcctg tgatgagctg tccagcatggtcctggaact caggggcctg cgcaccattg 49500 tgaccacgct gcaggacagc atccgcaaagtggtcagtgg cctctgcacc cagcccgtta 49560 gcatgaaccc tggaaggttt atcgcagatggtcccaaatg actgaaaatg cggggggaca 49620 ctaatgatat tctctcccat ttagactgaagagaacaaag agttggccaa tgagctgagg 49680 cggcctcccc tatgctatca caacggagttcagtacagaa ataacgagga atggactgtt 49740 gatagctgca ctgagtgtca ctgtcaggtaagggaccttc accagccaga ataagaatcg 49800 acggcttttg tttgaaccta catctttggggaaataccct aatcgccaca gagattcttt 49860 ttatgttcca tggcctgata acaaagtgttttttttttct ttaagatgca attatggcat 49920 ctataagttc ttctttaaac tctcagatggtttatcaaag tattaattat accccaggct 49980 aagtatctaa gagaatgaat ctactaagacatctgaataa tttacctacc ttagttcaaa 50040 gactaagtgt tgaatgtcag gtaaataaaacctggttgga acattcattt ttttttccca 50100 accctgattt atattcagct ttggcataggttcttttagt acagtagcaa ttgtttttca 50160 aatcacttag tctgacactg ttaggatgttactgtctgct ttgctcagat ggcactgcca 50220 ggtgtgtgat tcactctagc atccccgcatgccagtggcc agtgagtttg gaggttcacc 50280 tggcttatct attaatagca aagtatttgaatttgcttaa atacaaatca cagatgactg 50340 gctgtgggaa ccaacttcat taaattccctttcttttcca atataaagac agatcacaga 50400 tctatagatg aggaaaaagt gcttatagatcttccttcct ccccccaaac acataatata 50460 aaaatgtaaa agtcctggct gaattttctgagtttgattt cagacataga tttgacccat 50520 gatgtttgtt ggttcctctg ctataaattctttgtactgt aacttccacc cttgactaag 50580 tagcaattaa aaagaacttt ccaaggaatggagtgacacg taaactagca ctagaactgt 50640 ctctacgata gtggtggctg caaatatgacagagacagat ctatgaaaat cattccacta 50700 gagcattgac agccctgacc atgttttggcttgtaaagga aatgttacag gatgtagact 50760 tctgtatgca acatgctcag cagcctgttcccctctcact ctcttgccct gcagaactca 50820 gttaccatct gcaaaaaggt gtcctgccccatcatgccct gctccaatgc cacagttcct 50880 gatggagaat gctgtcctcg ctgttggcgtaagtttctca aatggcatag ctattcttca 50940 gtgactaggt cagttctaca tacatcctagacagccgagc acaacactgg aaccccaccc 51000 ccatctccat catgcttcca ttcagtttcttgctgcagca gctggttaac tgacagctta 51060 tctgctgtac agagccaaaa ctccattatgagtacatcgt aattacccat ctggattgca 51120 gtaatggcag tgaagatctt ggtatagctcattaattctg attttttgct ttgaggaaaa 51180 gagatgaaga cgtggcagca acagctctaagttgactgct tttagaattt gaccttagtg 51240 gcacagagtt aagaaaaatc tgctcaccagagatattgac aggcttactg tgtaagcatt 51300 ggcctgatcg ggcctggaag ttttgcccctttcatctgta gggataagag ggcctaagag 51360 aatagcagca atctgagctc tagagcaccaaaagaaataa ggcacgatat cgttccatgg 51420 cacgttatcc ctggcttttg ttaccagtaaaatgaatgac tttgctgaga caatacttag 51480 tgtagcaggt acaagttata aaagtgattcattacaacta agataaggaa gaaaacagga 51540 aaacagcact tgcccttcac acacacagaaacacacatgc acacacacac aaacacaccc 51600 ttgtcacagc tcagaataac caattccatccagggatcat attagcgata ttggcactgt 51660 aatggtcgtt tttacattta ttttctgttccgcattcact taacatgtgc gttaaaagga 51720 acaaagctag ctggaggtag caaagttttgaacttgaatc agggattgct aagaaaactt 51780 acaactaatg accacagaaa gtaagttctttgtcttctgt ctgatacagc tagatgtgat 51840 tgtagccgtg gtgcctgggc agatggtaaacaaacatgct gtcctcttat gacaatatgt 51900 gcagagaagg ccccaaacgc tggggtgagaggaacaacaa actgactaac tcacggccga 51960 gttggagcca gaaccaacaa cagctcggctgccttcacac tcatctgtca aacacatggt 52020 gtatggctcc cctgacagaa atgactcctataagcttcct catactcgcg cattcttgga 52080 agtctgagac tggaaatgcc tacccagatatgttaagtca gtaaaggaaa actcatttgc 52140 aatgctgaga ggagaaagaa aatcaatgaataggagagca tacactgtgg gaaggctcag 52200 ctgtactgag ccaggcaaag catccgattaccccactgct gtacacatca tgacctggga 52260 taaacccctg ggccgttggc ctaagaaatatgttgctttt catcctgtat aactgcaact 52320 ttgtgccggc tcatattatg ttctagagagctcatgtaca ccatggtgac catagttaat 52380 aatactggat tgtatgctta aaatttgctaagagagtaga tcttaagtca tcaccaaaaa 52440 aagtaactgt aagatgatgg agatgtgttaattagcttga cggtggtaat cacaatatat 52500 gtgtatatca aaacatcaca ttatacaccttaaatgtata taatatttgt caattatacc 52560 tcagtaaagc caaaaagaaa taaatatattgcttttcacc ctctggcaag tggagggccc 52620 tgaacagagc cctctaaaga acagcttgtcctaatcatca cgtacctgat gaacctctgt 52680 ttcccctgca gccagcgact ctgcggacgatggctggtct ccatggtccg agtggacctc 52740 ctgttctacg agctgtggca atggaattcagcagcgcggc cgctcctgcg atagcctcaa 52800 caaccgatgt gagggctcct cggtccagacacggacctgc cacattcagg agtgtgacaa 52860 gagatgtaag catcttagcc tctcagggacgtggagaact gacctgtcct tgttgtcgtc 52920 accaagggca gctccacagc atgtatattaagaacacggg ttctaggatc tctggatccc 52980 aatcccactg cttagctgtg cacccttgaacacaacttac cctctctgag gctctttcct 53040 catttgcaac ttggagataa taatagttcttgataagttt atcttgagca ttttatttag 53100 atactgcatg taaagcagtt agcactgagttttggtttat ggtaagcacc ctgtaaatgt 53160 taggatatta acagcaaccc ataaaaacagcgaacttatc cgttctctgg tagaaaacat 53220 tccatgttga catttgggca tgtggccaggagccaatttc taccgtacac tgggtttagt 53280 tgccattgac catttcctgg aaatacttctgcgtgctcct gatgggagcc tctatttggc 53340 tgtctctaag ccaaaaccat ttgtgaccatcaactctgta ctttagttaa acaggatggt 53400 ggctggagcc actggtcccc gtggtcatcttgttctgtga catgtggtga tggtgtgatc 53460 acaaggatcc ggctctgcaa ctctcccagcccccagatga acgggaaacc ctgtgaaggc 53520 gaagcgcggg agaccaaagc ctgcaagaaagacgcctgcc ccagtaagtg tgaggtccgc 53580 tgcaagggtg agcatgggca gcagctctgcccagctggtt gcctggcatc tgcagcctgc 53640 agttcagtgg gtcatagagc aggaaggttacctactagag aaacaaacag aagcaaagtc 53700 ctgcaggctc agcaacttct tttaatgaaaaacaaactca ccctcttccc cagcattctt 53760 tccatgtgtc agagaagcag aggtttcttgaacgggctta ggagagtcta tgacaaggga 53820 gggatttgaa agttgatctt aattgttgcctgtggttcat cttcttacag tcaatggagg 53880 ctggggtcct tggtcaccat gggacatctgttctgtcacc tgtggaggag gggtacagaa 53940 acgtagtcgt ctctgcaaca accccacaccccagtttgga ggcaaggact gcgttggtga 54000 tgtaacagaa aaccagatct gcaacaagcaggactgtcca attggtgagc cacgcagccc 54060 aggatgaaac gacccaggag ctttgctcttttactgaatg ctgcagtcag cattcgagga 54120 gattccagct tggttagtcc tgagcgatttgattgctcta agatgcaggt ggacaacata 54180 atcccaacaa gttatcggtt ccctataccctataatatct tacactgtgt taagtgccca 54240 gcatggcagt atggcagctt agaccaaccatttactgtga ctgtctctct ctccttgtct 54300 cagatggatg cctgtccaat ccctgctttgccggcgtgaa gtgtactagc taccctgatg 54360 gcagctggaa atgtggtgct tgtccccctggttacagtgg aaatggcatc cagtgcacag 54420 atgttgatga ggtgaggaac tgatggggctccgagtttct ggatctagga aagcagctaa 54480 cctgtgcagt cgcttcctta tggcagtgacttctaaacat gatgcacgct cttatttcct 54540 ccatagtgca aagaagtgcc tgatgcctgcttcaaccaca atggagagca ccggtgtgag 54600 aacacggacc ccggctacaa ctgcctgccctgccccccac gcttcaccgg ctcacagccc 54660 ttcggccagg gtgtcgaaca tgccacggccaacaaacagg tacagtcaac tagacgagta 54720 aaccagagga caggagagct gtccttgaccaaaataactg ggagcgggag gaatgtaatt 54780 tcataccctt caccaaaaaa aaaagggcgaggagatgaat gtacggtcta gttttagaaa 54840 cgtgattaga aaatccatgg taaatcctgcaggggaaaaa cagtcttcca tatttaaaaa 54900 tgctgctctg gaataagttg tgagcagatggacttgtaaa cgcctaggtg ctgagcaaat 54960 tcaagaaaaa taaacataaa gcaaagtttgcttatagcct cagggagaat ggggagggac 55020 agaggtaacc cacactcttc caaatggagcctctgtctac tcagagatga cagggatctg 55080 gattcttgtt tccatgatat ctgaggattctcaaaagctc tgtgtaacag cagcatggtg 55140 taccctcagg tgtgcaagcc ccgtaacccctgcacggatg ggacccacga ctgcaacaag 55200 aacgccaagt gcaactacct gggccactatagcgacccca tgtaccgctg cgagtgcaag 55260 cctggctacg ctggcaatgg catcatctgcggggaggaca cagacctgga tggctggccc 55320 aatgagaacc tggtgtgcgt ggccaatgcgacttaccact gcaaaaaggt agagccaggt 55380 cctttgtgtg cctccagaaa gagggcccatcaccttatca aaacacaggc taaggaattt 55440 taaatactta aagtttggac atgaaactgaaagattaaat ctcccaaagg gagaggggca 55500 ctaattccta ttaaaattaa atcacttggtgccaaaaaca tgaacagggc tagctctttt 55560 tgggcaactg tgttccatag tcaactcttctgtccaaaaa aaagttcagt actctatcca 55620 aataccagca aagcatgact tgtgttcacaacatctataa agcctagagc atttacacaa 55680 attctgtatg tatatccccc aagaaaagttttattttctt gtcttcccat aaaaaaaata 55740 tgctgttact gagccaaatt ctgttctttttctatttgga attccaggta cactccctcg 55800 tgcatgagct cagcatgagg aaggcaactgaagcagtgat atatatcttc tctttcctag 55860 gataattgcc ccaaccttcc caactcagggcaggaagact atgacaagga tggaattggt 55920 gatgcctgtg atgatgacga tgacaatgataaaattccag atgacagggt aaaaacagtt 55980 ttctatccct ttttcatctt ttcagttcagcaacagcctg aaacactttg ggattcaagg 56040 aaattacatg gctatagcaa aaaatataccaaatcaatac acaggataat tagaaattat 56100 tcattgtgtt ccagtagttt aaggatgtagatgttgccaa gagaattttt aaatgagggt 56160 tttgtttttc atcagaactg tttttctctgtacttgagaa attataatgc ataaacaaat 56220 gccactttgt tccctagatt catttcaaatgtcacatcga aattacagta aaattgactt 56280 tgggcacact atgaactgag atgatgggattatattctac atctcactaa cttctaaccc 56340 acagggatcc atttttttaa ctatgtccttttaacttttg tagtgatcgt tttacactga 56400 gtgatcaatt agcctatcca ctaggtagaaagtattgctg attttcacag ttttagacat 56460 attatgcaca tggtttgagg cttgagctgttttcaaggac aacattgtta agtgctccat 56520 ttcttctctt tgcaggacaa ctgtccattccattacaacc cagctcagta tgactatgac 56580 agagatgatg tgggagaccg ctgtgacaactgtccctaca accacaaccc agatcaggca 56640 gacacagaca acaatgggga aggagacgcctgtgctgcag acattgatgg agacggtaag 56700 gtgctgcctg atcagagggc ccgcgggagacagggacatg cacagctttc caaacgtact 56760 tctgtctagt cctggctatt agtatgcactttggtggaaa catccaaggc tggagagccc 56820 agctcttatt tgtcccttgt tctcttcaggtatcctcaat gaacgggaca actgccagta 56880 cgtctacaat gtggaccaga gagacactgatatggatggg gttggagatc agtgtgacaa 56940 ttgccccttg gaacacaatc cggatcaggtaggtggatgg actcctttca gagtctttca 57000 gtaaactgtt ggaatatccc tttcatggcctttgaaaaat gagcttaaca aagttcaaac 57060 actttcatta ctgtggctcc ctggcttctcatagccaact ggaatacgtt catgtaaata 57120 atttgcctga tgtgagctcc ttgcacagagtctcacagat ctttagcatg aagatctggt 57180 aacttcagtc ttaccttctg taagaagggctactggactt gctcagcatc gtctaccatt 57240 gttaagacaa attatacaaa gcagacatctgtgactgaca actgtacaat aatattaatc 57300 atatgtaata ctaagagatg gaaaaataaatgtgcataag caaaataacc attttcatgg 57360 ctaatgatgt aatctagcta taagaaaatcatcaaactaa ggatacatcc aaaaccaaag 57420 taatttatcc ctgtcataaa cattacccagacagtataca acacagggac cagtggtctg 57480 tttccatctt ttcaggactt ttcagagaagtcgacgtgtc aacagtttta caactgaaac 57540 cttctaagga aattctctgt tataaagtatccagaaaatt tcatttaaca ttaagatgta 57600 aacagttaac atttcatttc atctttagtaattaaatatc actctatttg accttattta 57660 gaaagtttta tatgtaaaac atttttaaattatcccttct cagattttta tacgaaaaca 57720 aagataaagg aaaaaaactt ttaaaatgtaattgctacta ttgctatctt tctgcaggag 57780 tgtgtaaata gacatgacac cccactggctgtatcaaaca atggagaatt ttccctgtgg 57840 tgggggcata agttatcttt aacatgaatggtttatactg caatttaccc tccatttaca 57900 tctctctttc agctggactc tgactcagaccgcattggag atacctgtga caacaatcag 57960 gatattgatg aagatggcca ccagaacaatctggacaact gtccctatgt gcccaatgcc 58020 aaccaggctg accatgacaa agatggcaagggagatgcct gtgaccacga tgatgacaac 58080 gatggcattc ctgatgacaa ggacaactgcagactcgtgc ccaatcccga ccagaaggac 58140 tctgacggtg agtcatggga gccactttctaagacaggga ctgctggcac agctgtgtag 58200 attgaagaaa tgaaaccaag gctcaaagcatttgacagga tgaagggacc aaatgccaac 58260 ttagacaaga tagtgacatt tctgacaccagtaataataa tagcacttta gaattttgct 58320 gaactcttgc ttttttgacc tcaggcgatggtcgaggtga tgcctgcaaa gatgattttg 58380 accatgacag tgtgccagac atcgatgacatctgtcctga gaatgttgac atcagtgaga 58440 ccgatttccg ccgattccag atgattcctctggaccccaa agggacatcc caaaatgacc 58500 ctaactgggt tgtacgccat cagggtaaagaactcgtcca gactgtcaac tgtgatcctg 58560 gactcgctgt aggtgagtag cgagttcttagatcctaaga gactgatgca tacatgggga 58620 aaaacaaata taaaacctgg cagttgtacctatccctgtg ggtgctgagg atgtctagga 58680 acatgatgga gaaccttctg aaggctgcaggttttaacct ggctctgggc tcttcttcca 58740 ggttatgatg agtttaatgc tgtggacttcagtggcacct tcttcatcaa caccgaaagg 58800 gacgatgact atgctggatt tgtctttggctaccagtcca gcagccgctt ttatgttgtg 58860 atgtggaagc aagtcaccca gtcctactgggacaccaacc ccacgagggc tcagggatac 58920 tcgggccttt ctgtgaaagt tgtaaactccaccacagggc ctggcgagca cctgcggaac 58980 gccctgtggc acacaggaaa cacccctggccaggtaagaa gcaaagccct ggaacagaga 59040 gagagcttat gggtgcctga ctagcactggggatgctgtg ctttgaccaa gactctgacc 59100 agggagtctt agaaagttcc cagcatcaccagctgcagca ttgaactctg ctttgtaaaa 59160 acataatagt gttgaaaagg gagcttgaccaagaattgcc ctgcaaatcc taaggtgcct 59220 tcagcctttt caaacaaaaa aacctccttccctcctctct gtctgcttta tatgtgtgct 59280 cagtggcaca caacaaatat gagaggacttggaaaaattc cccattgcag ccctctaact 59340 tagatcagct cagtaccttt caagcattgtttctgatgga atgaaataga aatctttacc 59400 tgaaggagct gtgtttcaac ctttccttttccttttcctt caggtgcgca ccctgtggca 59460 tgaccctcgt cacataggct ggaaagatttcaccgcctac agatggcgtc tcagccacag 59520 gccaaagacg ggtttcatta ggtacgatcatactgattca ctttcactta cagtcacact 59580 gagggacaaa aagacaaaaa gtattaaatagcattgtcac taaacaagat tttttttccc 59640 tgcagagtgg tgatgtatga agggaagaaaatcatggctg actcaggacc catctatgat 59700 aaaacctatg ctggtggtag actagggttgtttgtcttct ctcaagaaat ggtgttcttc 59760 tctgacctga aatacgaatg tagaggtaagagcaacatca ccatgaatgt acactggaca 59820 tctctatttc agactaatat caaggatgacggttatgggg gagtccagtg taaagactgt 59880 tttggagaca gggttatttc tattttgcttttgaggacac aaggacaaaa atggaataat 59940 gcctaggcac tgtggcttat gagttcctgagtccttagct ataatattag tttgcttagc 60000 aatctctgtg ctcctcatac acagtgcaaaggtaagctgc agaaaagctc ctatataatt 60060 tggacttcat taataatact gttctttacaattatatatt tatatatctt actgcttatt 60120 gttttatgca tgccatcaca gcacgagttagcattcccaa cttttccctg tatacaaaga 60180 agggagagga atgttgcttt catattggcatgttaaatta atgttcacta ttaaacttag 60240 catttttttt cctcattctt ttttacttagtcattactta agctcgctga gtccaacact 60300 ggctctacca caaaataagt gcttaatatatatttactgg gccaaggcca gatacctaaa 60360 agatcaatga cagtagagaa agatggttgattttactcaa aaaaatctaa attattaatg 60420 taatttttga gtccaaattt ttaaaataagactccctaaa ctgttaacat tgaaagcctt 60480 tggaaagcat aatatatgtt ctggaaggttcacgctgtgt cggtctccta gcatcaatgt 60540 cagctaataa aattaaatgc taatgtgcttgaacaacctt aaaattaggc ttttgtcatt 60600 agaaaagtag agctattcct atgtggttaacttattaact aagatgtcta tgcttttatg 60660 aattagtttt catttgtata tttatttatatttgtttatt taacagatcc ctaatcatca 60720 aattgttgat tgaaagactg atcataaaccaatgctggta ttgcaccttc tggaactatg 60780 ggcttgagaa aacccccagg atcacttctccttggcttcc ttcttttctg tgcttgcatc 60840 agtgtggact cctagaacgt gcgacctgcctcaagaaaat gcagttttca aaaacagact 60900 cagcattcag cctccaatga ataagacatcttccaagcat ataaacaatt gctttggttt 60960 ccttttgaaa aagcatctac ttgcttcagttgggaaggtg cccattccac tctgcctttg 61020 tcacagagca gggtgctatt gtgaggccatctctgagcag tggactcaaa agcattttca 61080 ggcatgtcag agaagggagg actcactagaattagcaaac aaaaccaccc tgacatcctc 61140 cttcaggaac acggggagca gaggccaaagcactaagggg agggcgcata cccgagacga 61200 ttgtatgaag aaaatatgga ggaactgttacatgttcggt actaagtcat tttcagggga 61260 ttgaaagact attgctggat ttcatgatgctgactggcgt tagctgatta acccatgtaa 61320 ataggcactt aaatagaagc aggaaagggagacaaagact ggcttctgga cttcctccct 61380 gatccccacc cttactcatc acctgcagtggccagaatta gggaatcaga atcaaaccag 61440 tgtaaggcag tgctggctgc cattgcctggtcacattgaa attggtggct tcattctaga 61500 tgtagcttgt gcagatgtag caggaaaataggaaaaccta ccatctcagt gagcaccagc 61560 tgcctcccaa aggaggggca gccgtgcttatatttttatg gttacaatgg cacaaaatta 61620 ttatcaacct aactaaaaca ttccttttctcttttttcct gaattatcat ggagttttct 61680 aattctctct tttggaatgt agattttttttaaatgcttt acgatgtaaa atatttattt 61740 tttacttatt ctggaagatc tggctgaaggattattcatg gaacaggaag aagcgtaaag 61800 actatccatg tcatctttgt tgagagtcttcgtgactgta agattgtaaa tacagattat 61860 ttattaactc tgttctgcct ggaaatttaggcttcatacg gaaagtgttt gagagcaagt 61920 agttgacatt tatcagcaaa tctcttgcaagaacagcaca aggaaaatca gtctaataag 61980 ctgctctgcc ccttgtgctc agagtggatgttatgggatt ctttttttct ctgttttatc 62040 ttttcaagtg gaattagttg gttatccatttgcaaatgtt ttaaattgca aagaaagcca 62100 tgaggtcttc aatactgttt taccccatcccttgtgcata tttccaggga gaaggaaagc 62160 atatacactt ttttctttca tttttccaaaagagaaaaaa atgacaaaag gtgaaactta 62220 catacaaata ttacctcatt tgttgtgtgactgagtaaag aatttttgga tcaagcggaa 62280 agagtttaag tgtctaacaa acttaaagctactgtagtac ctaaaaagtc agtgttgtac 62340 atagcataaa aactctgcag agaagtattcccaataagga aatagcattg aaatgttaaa 62400 tacaatttct gaaagttatg ttttttttctatcatctggt ataccattgc tttattttta 62460 taaattattt tctcattgcc attggaatagatatctcaga ttgtgtagat atgctattta 62520 aataatttat caggaaatac tgcctgtagagttagtattt ctatttttat ataatgtttg 62580 cacactgaat tgaagaattg ttggttttttcttttttttg ttttgttttt tttttttttt 62640 ttttttgctt ttgacctccc atttttactatttgccaata cctttttcta ggaatgtgct 62700 tttttttgta cacattttta tccattttacattctaaagc agtgtaagtt gtatattact 62760 gtttcttatg tacaaggaac aacaataaatcatatggaaa tttatattta tacttactgt 62820 atccatgctt atttgttctc tactggctttatgtcatgaa gtatatgcgt aaataccatt 62880 cataaatcaa tatagcatat acaaaaataaattacagtaa gtcatagcaa cattcacagt 62940 ttgtatgtga ttgagaaaga ctgagttgctcaggcctagg cttagaattt gctgcgtttg 63000 tggaataaaa gaacaaaatg atacattagcctgccatatc aaaaacatat aaaagagaaa 63060 ttatccctaa gtcaagggcc cccataagaataaaatttct tattaaggtc attagatgtc 63120 attgaatcct tttcaaagtg cagtatgaaaacaaagggaa aaacactgaa gcacacgcaa 63180 ctctcacagc gacattttct gacccacgaatgatgccttg ggtgggcaac acgattgcat 63240 gttgtggaga cacttcggaa gtaaatgtggatgagggagg agctgtcctt gcaatgttga 63300 gccaagcatt acagatacct cctcttgaagaaggaataat aagtttaatc aaaaaagaag 63360 actaaaaaat gtaaaatttg gaaggaatccataaatgcgt gtgtgtctaa atacaaatta 63420 tcatgtgaag aaaaggccca agtgtaccaataagcagacc ttgatttttg gatgggctaa 63480 ttatgaatgt ggaatactga ccagttaatttccagtttta atgaaaacag atcaaagaag 63540 aaattttatg agtaggttaa aggtctggctttgaggtcta ttaaacacta gaaaggactg 63600 gctgggtgag ataaaatctt ccttgttgattttcactctc attctataaa tactcatctt 63660 tctgagtagc catgatcaca tacaaatgtaaattgccaaa tcattttata gtaccaaggt 63720 gaagaagcag gaactagaaa gtgttgataatagctgtgga gttaggaaaa ctgatgtgaa 63780 ggaaataatt ctttgaaatg gcaaagaattaaataccatc attcattatc agaagagttc 63840 aacgtttgaa gtgctgggag ataattctaattcattcttg gatagtgaag caaaactgat 63900 tgaaaatacc aagataagac agaaaaagtgactggaaaga ggagcttttc ttccaggcat 63960 gttccagttt caccctaaga ctgaccttcaaataatcagg ttgtactgaa ataaaggact 64020 tgttaaaaat taaaattatg tcatcgagatgatagctttt ttcctcctcc aacagtttat 64080 tgtcatgtgt tgtgggagag ctcgagtgaagagcaataaa ctccaggtct tataagaatg 64140 tacatacaat aaaggtggtg ccagcagtttttttttttct aaagagtcac atgtagaaaa 64200 gcctccagta ttaagctcct gaattcattccttaaataaa ttggctctct ctctcttcta 64260 taatttcttt ttctttttat ttttgagatgaagtcttgct ctgtcgccca ggctggagtg 64320 cagtgacaca atctcggctc actgcaacctctgcctcccc ggttcaagca attctccctc 64380 ctgcctcagc ctcccaagta gctgggactacaagcgcccg ccaccaagcc tggctaattc 64440 tgtattttta gtaaagacgg ggtttcaccttgttccggac aaacactaag ccctaaaggg 64500 aaatccaaaa taaaaacatc tatttttaataacactttct atctaaatca gggtgacttt 64560 ttaaaaaaaa tccggaagct ttttgttgaattacgttaca gacttagtta ccagtccttg 64620 ttagagttac cttcagttga catgctgtgaatggtcccac ctcttttatg gcagaattca 64680 ttacttaaaa taactctatt ttcttcccccttacctaaat aacagaaagg ctcactatgt 64740 cccaaatatc attggcagaa gcaaactataaagtcataag ccctttgcag tgcaagtcta 64800 gaaataattt tactctgtgt ttctttctgtctccctcttc cctccccttt ttccttccta 64860 ccctcccctc ccctccctcc tccttctttctctctctctc tctctctctc atacatacaa 64920 acatcaaata ccagaaaagc atagatttagaaagctgaac tcctctgaaa cgctgacgga 64980 caacactggc ccctctctgc cccacaacaaattctcaccc atatcacctt tgccctctga 65040 ttcttcccac ccaaacccta aaacacagagtgtgcctcat ggcattccta tgagaatcta 65100 aaatcacaac ttttaactta tacttccatcccacagcaaa actcttggca tcaagataaa 65160 ttatacgtag cacattgaca tcagcatgaacaattgctat tttaaatgaa caaatagtaa 65220 actgaaatag tgtctggaaa aagaaactgcctatcaatac ctctgaaaat agtagctata 65280 gaattcctca ttttaaaaag tcaaggaaattccatataaa caatcattaa tgttattcag 65340 tatcagtgga tgaacatgtg ccagcaaaaacaatgttatg tatacatcac tcaaatttta 65400 ttaaaggcag cacaatagcc cgctcattttttgattcaga aataaactgg acttccaatt 65460 ctaatgccaa ctcatcttat ggaatgtcaacacaaaattt tctcatgaat ctaagttact 65520 gacttgtaac taaaccatct ctcttcaaatagtaccgcag actgtctttt aaagtagctc 65580 atctttattt gatgcaatct aaaaggcccaaactctgaaa gaaaataaga aactattgtt 65640 aactattatt aacaaagtaa tatatcaaataattcactca aactcctact taagaaacgt 65700 gtcatcacca ataagatagt aaaggctcttctaatattgc tttgttgaga caattcattt 65760 catcttatgg caggttgtac cagtttcacagacatgagac aagtgggaag aaggaaaagc 65820 agggccacag caaaaattcc cagtgaccaccatgatctgt ctgaatcaca gtagaggtga 65880 aacatacaaa attgaataca ggacaatcacagcacttcaa cgagttttac cctaacacgt 65940 atacattaaa atgttggttt ttataatgaatcctaatact gtaagattag caataaattt 66000 ataaacaatg gtcccagaaa ttttaatgagcaaaaaccac tgaacaatta caccccaagt 66060 ctcaaaaata tcaaggaaat ataatccacaaagagcgact ccaaatgtca aaatcaataa 66120 agaatagtct ctgcagtgca ttcattgaattcaaataatt caataagaaa tttaatttaa 66180 cttcattacc aactttctga aaagcacacccagcaagtcc ttgattaggg ttctttacat 66240 tcttctgctg catctttagt ctcctgctcatctgcacata aaaacaataa ccacagttat 66300 tagagattca gaaatgtatg cataattaagaaaaacaact cagcctataa aatattaaat 66360 ccctttacac atgaggcaat aatcacactctcatttaata cttactgaac agtcaccacg 66420 agctgcagac aaagtttacg agaaaaatagaaagatcagg gcgcctactc tcattgagct 66480 tacagctcag ggagaacact acaggttttggagctaaaat gattatggat gtggagacac 66540 acacagaagt taatattttg ctttctactaagtttctggg aagtacagtg agaccaaaag 66600 taaatgttat atgtagaata gataagagctgatcgccttt catttcttga agtgagaaaa 66660 aaaattgtga cattaacaga aaaggaatggaaatattttt ctacccttgg tgagaaggtt 66720 atcagaaaat gcagaattga caatagaactaaattttaaa aagacaaaga gaaacaggag 66780 aaactggaac cttcatgcac gactggcaggaatggaaaat ggtgcagtag ctttagaaac 66840 agtctggcag ttcctgagaa ggttaccatagacctggcaa ttccacttct aggtataagc 66900 ccaagagaaa tgaaagcata catccatacaaaaacttgta cacaagcgtt catatcaaca 66960 ttatatgtaa tagccaaaaa gtggaaacaacccaaatgtc catcaaatga tgaatggata 67020 agcaaaatgt gatatatcca tataattgagacttattcaa caatagaaaa gagtggagta 67080 tggatacatg ctacaacatg gatgaatcttgaagacataa tgtaagaagc cagtcacaaa 67140 aggccatatg ccgtatgatt ccatttatatgaaatgttca gactaggcaa attcacagag 67200 acagaaagac tggtgattgc ctagggctggggttggggaa aatgggaagt gattactaat 67260 acccaaaatg ttgcgttgtc gggggtggaaaaaataaaaa attgatagtg gtgatggagg 67320 tacaactctg taaatatact acaaacaattgaattgcaca cttttaatgg gatgaattgt 67380 aggaagctgg tgaacagtgg ctccccaaaactcacatcct catccctgca acctgtgaaa 67440 ggggtctttc tagatgcaat taaattaagcttctcgagat aagaagatca tcctggattc 67500 tctgggtggg ccctatattc aataagaagtgttgttatac aaaaaagaca gagggagatt 67560 aacacacaca gatggaggga ggcaatgtgaagacggaggc agagactgga gtgacgtggc 67620 cacaaagcca ggaatgccca tagccaggagaagctggaag aagcatggaa gcgttctagc 67680 gttctctcct agggcctcca ggtggcgtgtggccttggtg acaccttgat ttcaaacttc 67740 tggcctccaa aactgtgaga gaataaatttctgtcatggt aagttgttgt gacaaccata 67800 ggaaactaat acaaatatat gaattatatctcaataaagt tcttattaaa aaaagactca 67860 accttgtaat gaagggcaga tgacttgcagtttcagagca aagtgataaa tgtagtggta 67920 ctgtcaacac atggagaaga aatattaaacaggatgtaga ggcaggtggg atgaatgaaa 67980 agtggatcac aataccccca tgcatgaggtgggagatgat gggacataat tccacgcctt 68040 cgtaagtgct attctctcac tatggagtgttctttttgcc cacccagcaa ataaccattc 68100 atccttggag gcacaactta aatgccacctcccatccctc tatagtgttg tctatgtact 68160 ttgtcctgaa gctcttacta tgtttgtcccaccctactgt aattaagtgt ctgtctttag 68220 gcacttaaaa attttcatca atagaactagattgagtaac aaaaaatttt tcatcaatat 68280 acctagaaca acggtcctca actgggggaaattttgcccc caaagaacat tttttgcaat 68340 gtctggagac atgtttgtct gtcataactgcagaggacac tactgacatc aagtcagtaa 68400 aagccaggga ttccattaaa cacccgacaatgcacaagac agcccttcaa atgtccaact 68460 caaaatgcta acagtgctga ggtgaaaaactcttggaact tagaaaagca tctaatacac 68520 aggagaatct catgaaatgt ttgtggggtaaatcgatgaa tgaatggatg gcagatggat 68580 gatgaatgaa tagcatggag ccttggggttttaaagcatg gctgcacagc agaatcaccc 68640 agaccctgat ctcagaaact gacttaaatgactgctgcca gaacccaccc ctagaaattg 68700 acttaattca tctgggatgg aaccaagctgctaatctgtt ttaaatctca ccaggtgatt 68760 ctaacatgca gccagaggta agaggcagcactggctaagg aacagactca gtatagctta 68820 aatgccaggc cggctcaagg cagacggaagtagagagact gggcaatcaa aagtatgcaa 68880 agcatagata gttccatact ggttatatgtgtctccaaac aaaatattaa cgaagaaaga 68940 gtagagaact caagggaggg tcccagggcatgcttgctct aagaggaagg ttgcccaaaa 69000 gaatgacaag atggatggct ggagagatcacaagacaatg tggatgcagc agtatcttcg 69060 ttaaaaagaa atttaagaag aaaaaagcagacattcagca gatgggaaaa aaggtacttt 69120 ctgtacatta ttttcactac caccatttatttttgataat cctaaagtag aagtaagtag 69180 taaaccattt agtccggagc ttctcactctgtaacatgca cacaaatcac ctgcggggta 69240 tcttaaaatt caggctctga ttcagtaggtctgcagtggg cctggcactc tacatttctt 69300 tttgttgttg ttgttgccca ggctggagtgcaatgtcatg atctcagttc accgcaacct 69360 ccacctcccg agttcaagcg attctcctgcctcagcctcc caagtagctg ggattatagg 69420 catgcaccac catacccggc taattttgtatttttagtac agatggggtt tctccgtgtt 69480 ggttaggctt gtctcaaact cccaacctctggtgatctgc ccgcctgggc ctcccaaagt 69540 gctgggatta caggcgtgag ccacagtgcccggctgagac tctacatttc taacaagctt 69600 ccagttgatg ctgacaccac tagtccatagacctagttta cagtcacaat agcggatctt 69660 cagtaacttt ttactttacc caatttgaatgaagcaacag aaccagaagt ttccaataac 69720 tgataaagtg ataaggacat tacaattaaatatcaaaact ctcaagcatg cctttgttca 69780 ttttatactg ctaaaatgat agagaaaatgatcaacagca aatatttata tgaaaagtct 69840 acctcaagaa gaattaaaga aaattagttgaaagtaaatt gaagatgtaa attaaccaaa 69900 tataaactta ctatacacta aggttttatattaaaaaatg aattcacatt agaaatttct 69960 aatatggcat ctctgatatt ttgaccaaataaactctgga agagcattgg tttgactaga 70020 caaagggagg ttaagtcact aagggcattgaagaagcaac gtgtagcctt ggaaaagtaa 70080 tttaacaaat agtaactatg aggtctgaaggcacaccctg tttgagacaa acagaaaact 70140 attaaggcaa tgatgagtta aggacttccctaagagcaat ccaaaatatt tttaatggaa 70200 aagatcagct aatagggtca ctgtttctagaatctcaccg ctctcctgca aaccagtaag 70260 actatactat aacaaagaaa attttctcaaacaatctagt tcccttgaaa ctgttcaggt 70320 ggtatgacta aggacatata ccaacacacacccacttcaa accactgcaa cacccttacc 70380 aaaagtcaat ctgccggcaa ccaggcctcatccagcaagt tacaatcaat gggcaaaatt 70440 tcagcctcaa gtatattcag attatatagtcgtcattatt agtagaactt tttttttatc 70500 tgaggatccc aataagataa catacttagctttctttcat ctaaactgaa atctatagtt 70560 ctcaagtgtt cacagtgaaa tcctgtccctagcattaatt tagcagcctg gacattccat 70620 cccaggaact ctgatttcag caagtctggataggtccttg gacacagcta gaaaacaaac 70680 aagcaaaaaa ctccccagat gatcttgatactcactgtgg ttaggaataa tgctctgcag 70740 tttgtaacag ggcttctcaa actttattgccgggaagtat caccagggat cttattaaaa 70800 tgaaaattct catgccttag gttgtgggtgggcccttagg ggccctgcat ctctaacaag 70860 ctcccagtgg ttggattgca ctacaagcagaggagactta caacatggaa tggaaattca 70920 atgtctccaa aggaccaagc ccatgaagtagagagggcta ggtgggggct gtgtcctctt 70980 ggagaataca catgccttct aaggggagcatctgctactc agctcccgtg ggtggtggta 71040 gaacttgctg atttgtcaga aatgtcagaaatctagcctt ttatggaaaa atctcctgac 71100 aatgttcaat gttaaacgcc atgtaagccaaccaagtcca gtcccagagc taatcatttt 71160 atttgaatgt tctcatttga tcctcattacaaccctacag ggtattaagc catcttatat 71220 gatagacaag gaaaccaagg tgtagaggagagagagagaa attaactaac ttacctaaga 71280 tctccatcta gcagcagagc aaggattcaaacccaggtct tccctaccca gaacccatgt 71340 tcctaaccac taggccactt cccactggtgggtggtaggc caccctccct ctgctaatct 71400 aggttctagc ttgggattct gctacttacttggactccct taatttggtc ctttgagtaa 71460 ctaatctgct ctttggtttt atgttcacagtttcaattta gagtgaccaa ccattccagt 71520 ttgctagaga ctgaggattt cctggaacatggaacattca atggtaaaac caggacactt 71580 ctgagcaatc caagacagtt ggccactatagttttcattt aggggtatta ttttaaaaag 71640 aaaacaaaac ttacatgact cttgttgcatttactatagg cttttaaact gacacacaat 71700 tgtacatatc tatgggatac acagtgatattttaatacat acaatgtcta gtggtcaaat 71760 caggtaaatt gcatacccat cacctcatttatcaattctc tgtgttaaga acattcaaaa 71820 ccttctcttc tagctatctg aaaatatatattattgttga ctatagtcac cctacagtgc 71880 tatagactat tagaacttat tcctcctataaagctataat tttgtatcct ttaaccaata 71940 actccctatc ccttcctccc tgctaacttatacaattttt caagaatact gttcactcaa 72000 caggtccaac gagaaattcc actttgtttttcttaaagaa ttagtgcagt tctgtcccca 72060 ttttttactg cacaactgat tttgtgtccgtttttgccct attttactat gtttgttatt 72120 tgctaatacc atgttcatct ttattttatgaatttctgtt aataattagc tcaaatgctt 72180 agtaggaagg gagaatacta tacaagcataggtggctttt gctttgatta cctagaaagc 72240 aactcagtat tagataattt gattaaatactagactaact cacagacctg atcctttttg 72300 cttcggaagt gccagtacat tcaggattgtctctgaggtt aagtgtgacc tgacaacact 72360 taatccccac tctcctcatg ctcccacaaggaggaacttg cagtggaagc atcctggcct 72420 cacttcacac cctccacact gtccgtgggaagctaagttt gggagatgaa atggcatgct 72480 cagctcagcc cctgcctcct caggagtcagcacccagctc ccacctgacc ctgctcccca 72540 aggctccatg cttcgctcct ccaccctattggttttacat ggacaggtct ccacagatga 72600 tgcctgagat tttccatctc tgccagtgactgggtgagta cgtttatttt aaaggcatag 72660 cattggaagc ctgtctggct gcccacccggatcatgacaa tcctctacat ttcattagca 72720 ataaagcgaa cctccttgat ctccatttgtcagaatctat ctctctctca gtcggttctg 72780 acctgggtgg ggaagagaag agtgctagttatttgtcccg taaaacccca gtaactagtg 72840 agtacaatgc tgaatttact gaaccttgtattcaaaaatt caaataatac tggttaccat 72900 gaagcttaaa gcacaaacat tgataatgtgtttcacattg aaaattttta atacaactga 72960 taggcaaatc cttatttttc aataccaccatttaaattgg tcttcaaggt cactgagttt 73020 ggttttagtt ttgaagcctt atctgactctgccttttaga agcacaattg aaatagttgg 73080 aacagacctc agcacatcaa ggaaaagacaactacgtatc agtaatactt gctattgaaa 73140 gagaaaacaa taaatggctt ttcttattttcttcctgtct gcttccagaa atctaatccg 73200 aatcacactc ttgtgtatct atttcctgagaaggaggtag aacagaaatg taacagccaa 73260 acacgcagca actcctcatt agttatgtcttcaaagggct ttttgtcaag aaaactgaca 73320 cagcattcag ttttgatgca cacttttgtagaaagcacat aatggacaat ttagtatttt 73380 agaataaaat tgatcagcaa gctattttaataattataaa taatgaggtt agtaaaactc 73440 caacttcaga tattctaaca tgctcaaagtaataatgtaa gacctattcc ttagagctta 73500 ttatgtaata aacagaaagt aaaatatggttcacatattc acacctaaga caccttaatc 73560 atagtcacag ggttaattgt tataattgcatattaacatt atctggtata atcagagcca 73620 tgttctatgt ctccatagct tctttccattaagtcacatt aagttccaat tcattccatc 73680 ttgtcatgct ctacccagtg acagatatgcatcagtgtga ctcatgttca cataaaaata 73740 aagtcatatc ttagcaaatt acttttctaaacccccctat atatggactc catgggaatg 73800 agatcccata cacaagaaat gattgcatccaattttgtaa gtttgatttc agaagtcaga 73860 ggagattcag atactttcta ataaaaacactttatccaaa aagaaggaca atatacttca 73920 atttcagagt agaacaaagt tttcacagttcatttattaa atgaagatct ttggtgtgaa 73980 gaaagcaaac cacacccatc taactgaattgcatttgtaa gtgaactctg ctgctaaata 74040 aaaacattac agctggaagt tctactcaggaagatggcag aatgggtgtt gctcagctcc 74100 cataccccca acaaaaatcc aaccaactatttacagacaa gaatatcttc attaatactc 74160 ctgaactccc tgaacgtgaa aagctgagacacccccttgg accacataac tgaaaaaagc 74220 ctcattcaag gggtaagtgg aatagaggaatggtttcact ttgaccacat caccactccc 74280 ccaaaccctg ccacacctca agccagcacagcaccacaca cagaagattc ccctgggccc 74340 agagtttcta cagtgggaaa agagagaggcaaacattcag cttccccaac attctgggac 74400 cctgagcagg aagcccactc attattatctcccgggaaac actgggagta caggcagggc 74460 tagaccacct aggatcagcc agaaacaaagaacaaaagtg agactcacag aaacgagcac 74520 atggatcttg cagtggctct gcattctagccaatggaggc actccaccag agaaactggc 74580 caacagcatt agcactgtcc tgcaggaagtatagtcaact gatctgccag gcttcattcc 74640 ctagccagct tccccaccta gctctggtgctctccttaag ccttccccag gccaggcagc 74700 aagggcaggt cagtgattac ctaaggagggtagcaacggg ccccacccaa ccccagcagc 74760 caactagcct aaccctacca agccttcatgttctgcttaa ggcttgccag gctaggaggc 74820 aagtgcaggt caatgaatga atatctatggacaaaagtac ctggccccac ccaaccccag 74880 cagctgcatg gtgaccccac aaagcctcagtgctttgctt aaggcttccc tgagggaagc 74940 ctgcatccat gtgtttctct agacgatagcctggcccatc ctgaatggct gagcacaaca 75000 gcagaaacct cacccagtcg ctgagcccagcacacagacc tgcccagcta cagattaaaa 75060 acagcagtac ttcccagcca gggaaaacaacatgcaaccc tgtgcaatca gaggctatta 75120 cagagcccag ccagtagctc caccttaactgtggagtcca gctagtgatc tgtctcatca 75180 gaccacagag cacagccaga agttccacttgacctcaaag aacagccagc aggctggccc 75240 aacagcaaca ccaacagcaa ggtgtacctgtctggggttg ttaccagctg gcctatccat 75300 aatcaaatgc taaactaact agtgaaggtctaccactgtg aaagaaaacc ttcaaggcca 75360 aaagaggtgg ccatttcctc aaatgtgtagacaacaaggt ttagaaagaa tcagggaaat 75420 gtgacacaac aaaaagaaac taataaagccccaataatgg accctgaaaa aaatgtagag 75480 ctatgaaaaa agaatgaaga aagcctacataaatctatgg gacaccatca agaaaactaa 75540 ctttcttata ataaaattct aagaaagatatgataaagaa aaaggcccag aaaatatatt 75600 taagaaaata tggctgaaag tttccaaaatttgtggaaat atgacaacat ccaggtatag 75660 aaagctcaga gatctccaat taaattcaagccaaagatga gtgcaccaag acaaatcata 75720 atcaaactat taaaaaacaa agacggtcaggcgcagtggc tcatgcctgt aatcccagca 75780 ctttgggagg tcgaggcggg cagatcacgatgtcagcctg gccaatatgg tgaaacccca 75840 tctctgctaa aaatacaaaa atttgctgggcatggtggtg cgtgcctgca gtctcagcta 75900 ctcaggaggc tgaggcagga gaatcgcttgaacccaggag gcagaggctg cagtgagcca 75960 ggatcatgcc actgcagtct agtctgggtgacagagcaag actctgcctt aaaataaaat 76020 taaattaaaa ttaaaataaa acaaagacaaagaatcctga aagcagtaag agatcaggaa 76080 tgtataatat tcaagggagt cccaaaacagcaaccagcag atttctcagc agaatccctg 76140 caagccataa aagggtggga taatataatcaaagtgtaga agaaaaacaa aactggcaac 76200 caagaatact taactcagca aagctattcctcagaaataa gggataaata aaaactttcc 76260 caaacaaaca aaaactaagt aagtttatcaccactaggcc tgcctaacag gaaatgctaa 76320 agggagctaa gctgaaataa aaggctcctaattgatacat aaaacttata aaagcaaaaa 76380 gcactaaatg gtgtaagtaa gacatagtcatattcagaat aatactgtaa ggatgatgtg 76440 taaaggaatt ttctctgtat tatgagagttaaaaaacaaa actattaaaa acaaatgcag 76500 ctataataaa ttgttaaagg aatccaaattacaaaaagat gtaaaattta catctaaatc 76560 ataaaagttg tcggggggag ggcgttaaagtattgagatt ttatatgtga taaaagttat 76620 cagcttaaaa tagcctgtta taataagatattttgttgtt gttttttttt attttttaag 76680 gagatggagt cttgctatat tgcccaggcagggctcaaac tccttggctg aagtgatctt 76740 cccacttcat ccttccaagt agatgggactacaggtatgc acaaccatgc ccagcctgta 76800 caagatgttt tatataaacc tgatggtaaacacaaataaa aacctgtaat agttgcacaa 76860 aatataaaag gaaaagattc aaagcataccatcacagaaa attatcaaac cacaaaggaa 76920 agtagcaaga gaggaagaaa gaaacaacagctctacaaaa caaccagaag caaattacaa 76980 aatggcagta gcatgtcctt acctatcagcaattaccttg aatgtaaatg gattaaagtc 77040 gctagtcaaa acacatagag tggctgaatgggttgaaaaa atagtaataa aaggccaact 77100 atatgctacc tgtgagagac tcactttactagtaagaaaa tacatggaca aagtgaaggg 77160 atggaaaagt tactccatgc aaatgaaaaccaaaagagag caggggttag ctatacttat 77220 atcagacaaa atagacttta agtcaaaagctacacaaaga gacaaagaag gtcattatat 77280 aatgataaag ggatcatttc attaagaggacataattgta aacatataca cacccaacac 77340 tggaacacct aaatacataa agcaattattacatgatctg agagatagac tgcaatagta 77400 taagagtagg ggacctcaat accccactttcaatgatgaa gagatcatct agacagaaaa 77460 tcaataagaa aacattggac ttgaattacactttaaatca aatggacctc acattcatat 77520 acaaaacatc acaccctaca gcaagaaaatacacattctt ctcaagcaca catgggacat 77580 tctccaggat aggccacaaa acaagtcttaatgaactcaa gaggatttta atcatatcaa 77640 gtatcttttc tgatcacagt ggcatgaaactagaaatcta taacaagaaa tcttggtgaa 77700 ttcacaaata tgtggaaatt aaacaacatgctcctaaaca aacaataggt cagagggggg 77760 aaaaaaggaa atcaaactat attttgagacaaacaaaaat ggaaacacaa catatcaaaa 77820 ctaatgggat gctgacaaaa gcagttctaagtgggaaata taaaacaata aatgcttatt 77880 atcaaagaaa aaatctcaaa taaacaatctaatgttaaac ctcaaaaaac tagaaaaaga 77940 accaactaag tccaaagtta gcagaagaaagaaaataaca aatattagag ccaaaataaa 78000 caaaatggag actagcaaca atagaaaaaatcaacaaaac taagaattgg ctttttgaaa 78060 agataaacaa aattgacaaa cccttagctagactaactag gaaaagaaag aaagaaaact 78120 caaataaatc aaatcctctg aactcatcacaccatcatgt aatgggaaga taatgattat 78180 gaccatattt ttgtctcatg atagttttagcttatacaaa aagtcttaat ataaacgacc 78240 aaaaataaag aaatgcttaa attatgatacatcataagat acattgcagc catcataatg 78300 aatgatttag aagaatattt aataacacagaatgctcaaa atataaaaga aaaaaatatc 78360 agaatatatt ataagaagaa tatgaagacaatttaaaata cacaactaga aaaaagatga 78420 atgttaatat taacaatgaa taggtctggacaatgatttt atcttctaca cttttctgta 78480 tgtttcaaat agccttcaac aaatttacatttgttgtgcg attagaaagc aataacaaat 78540 actttttaaa gtctttaagc tcaaaacttctgcttattta aaacaaaacg aaaatgaaat 78600 gaaagaggag acattacaac tgataccaaagaaatacaaa gggtaattat gtaaaattat 78660 atgccaacat ataataacct agatattttattggataacc tagatgtgaa cagattcctg 78720 gacacataca tcctgccaag gctgaatcataaagaaacaa aaatctgaac agaccactaa 78780 tgagcaagga ggttgactca gtaataaaaagcctcctatc aaaaaaaggc ccagggccag 78840 atggctttac tactgaattc taccaaacatttaaagaaga actaatacca gtgcttctca 78900 aactcttcca aaaaactgaa gtggaagaaagccttctgaa ctcattttac aagaccagca 78960 ttacccagat accaaagcca gagaagaataccacaagaaa aaaaaattgc aggccaatat 79020 ccctgataaa cacagatgtg aaaatcctcaacaaagtact agcaaaccga attcaaaagc 79080 aattaaaaga atcattcaca tgatcaaatgggctttatcc ctggtatgca aggatggttc 79140 agcatatgca aattaataag tgggatataccacattaata aaatgaagga taaaaaccat 79200 atgataatct caatagatga agaaaaagcatttgacaaaa ttcaacacca tttcatgata 79260 aaaattctca acagattagg tatagaggtaaagtacataa taaaggccat ataagataaa 79320 tctgtaacta acatcatact caacggtgaaaagtagaaag cttttccaca aggatgccca 79380 ctctcaccac tttttttcaa catagtacttaatgtcctgg ccagagttac gcaagaaaaa 79440 caaataaaag gcatcctaat aggaaatgaagaaatgaaac tatctctctt tgctgatgac 79500 atgatcttgt atatagaaaa tgctaaagactccaccaaaa aactgtcaga agtgacaaaa 79560 aaaaatcagt aaagttacag gatataaaatcaacatacaa aaatcaggag catttctata 79620 cattaagaac aaactatcta aataggaaattaagaaaaca atcccattaa caattgtctc 79680 taataaaaaa aattaaccaa ggaggtgaaagaactatata ttgacaacta tagaacactg 79740 atgaaagaaa gtgaagaaaa agcaaatcaattaaaagatg tcccatgttc ccagcctggc 79800 ccacatggtg aaatctcgtc tctactaaagatagaaaaaa ttagccgggc gtggtggcac 79860 gcacctgtaa ttttggctac tcgggaggctgaggcaggag aatcgcctga acccaggagg 79920 cggaggttgc agtgagccaa gatcatgccattgcactcca gcctgggtga cagggcaaga 79980 ctccatctca aaaaaaaaaa aaaaaaaagaagatgtacca tattcatgga ctgaaagaat 80040 taatggttaa aatgttcaca ctatccaaagtcatctatag atttaatgca attcctatca 80100 aaaatccaat gtcattttca cagaaatataaaagaaacaa tccttaaatc tgtatggaac 80160 cacaatagac cctgaatagc caaagaaatcttgaccaaaa aagaagctgg aggtatcaca 80220 ctccttgatt tcaaagtata acacaattataattgcaaca acatgctact ggcataaaaa 80280 cagataagtc aatcaacaga acaggacagaaagcccagta ataaactcat gcatttatgg 80340 tcaattgatt tttgacaaag atgtcaagaatgcacaatgg ggaatggtgt tgggaaaact 80400 gaatatccat atgcagaata ataaaatttgatccttatct cactccatgt ataaaaatca 80460 actcaaaatg gattaaagac acaaatataagacccaaaac tgtaaaacta ctagaataaa 80520 acaggggaaa tgctccatga cattggtctgagcagtgact tttttttaat acgaccttga 80580 aggcacaggc aaaaatagac aaatgtgattatatcaaacc aaaaagtgtc tgcacagcaa 80640 agaaaacaat gaacaaagta aagagacaatctgtgaagta ggagaaaata ttttcaaact 80700 acacatctga taaggggtta atatccaaaatatataagga actcaaacaa ctcaatagca 80760 aaaaaaaaaa aaaaaattaa aaaataggcaaaagacctga acagacattt cccaaaagaa 80820 gacataaaaa tggccaacag gttcatgaaaaaatacttag catcattaat cattaagaaa 80880 atgtaaatta aaatcaaaac gagatgctacatcacacctg ttataacaac tattatcaaa 80940 agatgaaaga gactgggtgt ggtggctcacgcctgtaatc ccagtacttt gggaggccga 81000 ggtgggcaga tcaagaggtg agatcaagaccatcctggct aacacagtga aactccatct 81060 ctactaaaaa tacaaaaaaa aaaaaaaaaagtcaaactca tggaagtgga gagtagaatg 81120 gcagttacca gtggctgggg gatgaagagggggaaaaagg gaatggagag ttgttggcta 81180 aagggtacca gttttcactt aggaagaataaattttgaga tgaattgcac agcagggtaa 81240 ctacagtcat taatcacata ttgtatgtttcaaaataact aagagtaaat ttcaagtgtc 81300 ttaccacaaa aaaatggtaa gtgaggtgatagataatgta aattagctta atttactcat 81360 ctcacattgt atacatacat caaatcatcatactttaccc cacaaatgta tataattatg 81420 ccaatctaaa aaatattaat ttaaaaattacagcttgcga aatttcatgt aagaaacaaa 81480 aaatccaaat atttatatag aatgtgtgacttacataatt cttaagcaac attacctact 81540 ttcattggta atgtaaagaa taaatgtgaaccatattttc agtgttttat tctaaaactg 81600 gttatacatt taaattcttt tatttattaaacaggcactt ggcttaaggc agcactttag 81660 acaccatgga aaatgtaaca atgaattgcaagatagagtc tctgttttta aaaatgttat 81720 gcagttttaa ggaagagaag gtgagtgtgtatgtgatcct ataggaaacc aaagtgcttg 81780 gacattcaca gaagacagaa aacttaacagaggggaccag ggaaggctac aatcagagag 81840 gataaggttg gaattcaaat accattgaaggggtacctga acatttctgg gaagaagagg 81900 gcctgaacaa cagcagaagc taggaaatcaccgaaatcat tagaccagat tagctacaca 81960 ctgaataaca tttaggtgac tacttagagattgattagga aaagaacttc aggtcagatc 82020 atggagacac tgaacaagtt aaggaattctgattttgatc aacaggcatt ggagagccat 82080 agaaggaagt gtggttatca aagctgtgctttaaggaaat tcgtttagtg gttgtcaaca 82140 atggtaggaa gagaagagag acttgaggcagggaggtctg ggagaagggt tatggcattg 82200 gtccagttga agttgtagga agagaaaggaagggatgggc atgagagaca ccgcaggaat 82260 agaacaaaca acacctgaac actggaagtgggaagcaaga aagagaagta attcagtgat 82320 gactcagatt ttgctcctgg gtgactaaggaaatggaagt caaaaccgaa atctgactca 82380 gttcagagtt aatggattat agatttaataaggaatagat taatagatag aaatgtaata 82440 gccaatatat tttagagtaa tctacaatagaggtaaaaat tgaatctatt taaatagatt 82500 caccaattgt tacacagatt tctaacataaataattgaat atttgtttgt gtcgaaaact 82560 agttattgtt gtatcctgta ccatgaccattgcaagatga aacactagat cgtgacgaaa 82620 cacctgagaa cataggagtg gctcaggagaggaggtttgc atacagaaac taatataacc 82680 acaatgagtt acaagtttta cattttaaattcattcctta gaacaaattt tcatcactaa 82740 agtttgaaaa tactaaagtt tgaagttgaaatactaagca aatctattca aagtatttct 82800 ctccattcaa actaatttct cttttcctgtgaaaccaaca ttattttctg gatgacacaa 82860 ttaattacca tcaagaataa cgtatttcatagagtactaa cctcaacttt gatttcataa 82920 gagcattggg aaataggttt tatgtttgcatactcttatt gacgctacct gtgacttaca 82980 aacatacctt cttacccgac tctaatttaaaccataatac ctttatattg aggtgctttt 83040 aagaattatt taccaaaaca catgttcgccaagcctcacc tgctattgta ttctctggag 83100 aactgagttt cagatgttgg tcttctgatgaaaggctcac actgatacca tacagtggat 83160 catctaagaa ggagggcctt ttcagtctcccaatgccaag agtcttcgac ataaaatagt 83220 cttttgtgtc actaataata acgtccttggaaaattgaag gcatttcata ttctctgctt 83280 cagtgacaag agcttctgac atgttatgtccagtcaaggc tttagtgaga tagtagcctt 83340 tagaagcttc acattcttca ctctcaagcatatctgcatc ttctacctca gttttctgga 83400 cctttgtttc tgattcattt agcaatttagagatgatgga cctggaaagc tgggggaaca 83460 caggtgttac gtcctcaatg tcttccttttttctttctga agaaagttta atgatggatt 83520 tttgtttaag tatgcattca tccagaagacactttaactg ttcttcagag agacatgatg 83580 tggactctaa ctgatgaaac aaaccaaaagattacatagt cagttgactg agtaaacaca 83640 tttatttttg gtaggcatcc agtaatacaaatataaatgc aagtaacccc ttacacaaca 83700 cataaaaata gaaaaataat tcattgtaaagtatccaaaa ctgtagggga aatgtcataa 83760 tcctggcaaa atctttggtt ggtgtttctctgaccccacc catccctcag catgaatccc 83820 accaccatcc aattccttac tgggaaaaaaattggcatac aaggcagact acccctacct 83880 ctggggattc agcactccca tgtctgtccacaactatcag tggacaatac tgctgaagac 83940 agaaaaggaa gggtgaaggg tggtgcatcctgcaatgtgt cgttgctgca aagctacagg 84000 tctaggcaga ggccaggggc attccagaggcggtgtattt ggggagaatg atttgatcat 84060 gcatacttaa tctctgcttg taaaggttaccagggctaag tgtttcaacc aagaccttaa 84120 atatttaaaa acattttttt tctttcagcaatattctcag gaaataaatt tttaaaaatc 84180 aaatgaaagt agagagccat gtcccacaagacttcagctg aataccataa catttgagtt 84240 gcaacaatct caaaaaggaa tgagattaaaatatagtgaa acacactgaa aaaaaaatgt 84300 ctacttcccc aaaaaatcag tgtcttccatggtttacatg tggattatct aggaaaagac 84360 taatcctcct ttacaggggg atgaattatctgagaacaag aagtccccat aagaaaactt 84420 aatcatgaat ttcaatgact ttaatttctacttcagcaaa gtaaaataat gttattggcc 84480 tactctgaag gtacatagta aaaaataactaaatattctg catatgtaaa gtactgaaat 84540 aaaaagctat tactgatact agtatagttaattctccact attaggtaaa gcttctaatg 84600 caactaataa atactaatat gtaaaacaattttgtgccat gtttaaaaaa tattgtttaa 84660 aattatgttt aacttaccta tattaaaactagtttttaca gtggcaagtg tcactatgag 84720 tatgaggata ataagaaatt gtctggaaatatattcttag taattatttg gaattcaaaa 84780 ttcaggtgac tgaccctaag gttaatatacatgtcaagaa ataggatttg agatttggaa 84840 ttcaaaattc aggtgactga ccctaaggttaatatatatg gcaagaaata ggattagaat 84900 tatgtattag caaattctac ttttagtcactaggcatttc aggaatccag atatagtatt 84960 ttatccatct gtatccagaa tcaggagcattatctccacc ctactatgga aacctagtca 85020 agtagaaaat gaaggaagtc cttctatgacattagatacc taaaacacac tttcaaactt 85080 tgcttatgta aaattacata taatgtgtagacaattccct acgatttaga aagagcccgc 85140 tgagtgtcat ccttaccaaa catcctcatcaaagccttat tatgatgaaa tttttagaca 85200 ctagaaaaaa aatcagtcca tattagagcaggaggaggtc tctagggaaa aaagggtagg 85260 agggaggatt ggtagattat ccaatatgcttgaccatttc caaaagtaat attgatatga 85320 tagatgtttg gcagttctga tgatgtatttggctaaatac acagaaaatg aagcagaagg 85380 aaaatcaaag caattatgaa ctctggggaaaacaaaaagt tttacaaaaa aaaggaaaag 85440 cagtgacata tacacagaaa attaggcaaaagaaactaag ataattatga agtctaggac 85500 aaacaaaaag ttacatgaga aaggatgccataggctaata attgcttctg ctgtgaataa 85560 tatttacaca gtcaatgata atataaatactgatgactaa ggtaagcaaa aattgtgact 85620 taactatttt ggggaaatgt gacgagaaacaaggggtgtt tgcactttat tagttaaatt 85680 attgcctacc attacagcaa cccaatatataatgtctaaa actaatcaat taagaaagta 85740 agttattaat aaataatata gaggtaaaattaggaaaaac acaaaagatt taaaagcagt 85800 taactggaga aaatatcaag gggtagggaaggctgaggca aggggctgcc atttttattt 85860 aaaacatttt aacacaattt tttctttattactataacaa aagttaaaat taaatataaa 85920 aataaaagta atttaaatgt attcttttttttttgacatg gagtcttgct ctgtcgcgca 85980 ggctggagtg cagtggcgca atcgtggctcactgcaacct ctgcttcctg ggttcaagcg 86040 agtctcgtgt ctcagcctcc ccggtagctggaattatagg cacccaccac cacacctggc 86100 taatatatat atatatatat ttatatttttagtggagaca gggttttacc atgttggtca 86160 ggctggtctt gaacttctga cctcaagtgatccacccacc tcggcctccc aaattgttaa 86220 gattacaggc gtgagccacc atgcccagcccaaatttttt aataaaaatg cacttagaaa 86280 taaaatttaa aatatttaaa tgaaattcccaggtgattgt atgtttggac atcagaagct 86340 gacccatgtt tcacagagcc agaaatttatataatcttga agcaagagaa gggcatttct 86400 taaaaaaaaa aaaaaaaaat acaaaattacaaattcaaaa ttaggtacaa aagcgagttt 86460 cttttatttt agaatgtgaa aaagatcacaacaaattact ggggtcttgg ggagggttga 86520 gtccatttct tacagtatct ctttaggtcattgaccagac gtgcttacag aaatgtttgc 86580 tgattgcaac ctggcttcct cttcccaccccaaagctcct ataagtgaga ggccctgagc 86640 tgaatcttca ctggcttcat ggtaaattgcttctgggcac tatgagataa aacattacac 86700 atttcagctt gtgtgtacac agaaataagctggactacaa ttaccctgat cctcctgggg 86760 gaagcagctg gataggaaag aaaacacagaagactagaac ctgggcttct atccaagctt 86820 tgctgccttg agcatgtcac ctgagttctcaggggctcat ctcctttatc tgtaaaatga 86880 ggaactttag aagataatct ctacattaaattctaactct aaaattgctt tttatgaaca 86940 actacactaa aaataaaaat aaattttaaaataaactttt ctactcactt tagagtgtag 87000 cctgggtaga gttatgaaaa caaaataatagcaaaaagag aacttttatt tatttagctc 87060 cttcacaata tcaatcccga actcttataaaataactaaa aattctctgg cctcaaaata 87120 taagtaagag caaatgaaat acattaagccctcaaattaa tttaatttgc ttctgggtgc 87180 aatacagatt ggaaacttca acacgacccatctttctcat cttttgattc ccagcagagt 87240 cttgcccaca gtcaaagctc agtcaatgactatcatgaga attgaatgat tagagtgctt 87300 aaaccatttt ttaaggcact atattgatataatttattta caaatactga actgaactaa 87360 actagggctc catcacaaac atccctaactgccctcaata gttaaggaat catctaaccc 87420 acagcctttt tccagattgc tctcaaaaattctgagctaa taagcaccat aactgaacca 87480 ctcactgtgc aggcactgtc aagtaaccatctgttcaagt attcacctca aagtattcat 87540 ctttcgtgcc agctctaatt tcccagtattccataaacaa gttattattc atactattcc 87600 tatccttcat aaaactcaac atagtgcatctcagtcctca tttttccagg ccagaaccca 87660 atctcttttc actctgtata tgaaagcccctctcttccca ccttcatttt agtagctctt 87720 ttatgaatct tctttctcgt ttcagccaatacacacacat ttcatagtaa aagagtaaat 87780 ctattggatt tttcatcagg aaaagaatagaatacacatg cagttttagt tctgctgcat 87840 cttgcatctg gttggcacac taggttatgtcttacagaat aataataaat aggtagcatt 87900 cactaagcag actagactct gctctaggcataatacatgt attgattcct ttaattttgc 87960 aataaaccta ttaggtagaa actattgttatttccacttt gcagatgaag aaactgaagc 88020 actaagaaat gaagtaacct ctccaaggtcatatagcttc taaaccgtgg acttgaaatt 88080 tgaatccagg tagtctgggt ccagaacctaagtgtctagc cacatggcta cactgccact 88140 cgctgtaaat gagttctccc tctaccaccctccaagtgtc cttttcccaa ctgctgctca 88200 tttttctgtc cattctatca gatacagaaactctccccgc agaacagttt acttaacttg 88260 ggactttatt gcccagattg gcttttgtgtgtacgtttta tgcaaatgta cacagtgtat 88320 gttttatgca aacatatata gtgccctctcttttagctca ttaaaaaaaa aaacccagcc 88380 acagctccat tcccaggata cccactgtttacacatctcc acccagataa atgccagttt 88440 gatgtagaag ctaaatttga tgaggaatgcaacttgtaat tgctggagat ggaacacaga 88500 ctgtggatta cctcactctc atccctgccccacctcctat gctatatatc cggagtttct 88560 ccagccataa aaatctccag ttaattgggcaatggatttc ttgacaaggc aatcaagatt 88620 atgtccctgg tgcagataat cagaaagtctacccattata aaccagcaga agcaatcttg 88680 tcctttgatg taagcttact tcgggtgttttttatttccc ccaaacagca ctcctaaaga 88740 cccaccctat gaaaccaata ccattatcacaagtgagaaa agggtggaaa gctagaatgt 88800 tctaaatgat taacaaggag aatatggatgtacacagatt ttaatgacac ctagttctac 88860 ttccaaataa gcaactaaaa aacattccaccgtttgctta atgacgaaga tataagttta 88920 tagctctcta ttgcaaccac tactggcaacacctccgtag tagtctattt tagtcaaggt 88980 tttttggcta taaggaacag aaacccttatgtcaaaaaaa gaggtggtag ggttgaggtt 89040 tgctgtaaga tcaaagaagt catttcatggacaacgacaa gcaggaaata agagtataga 89100 ctggcctttc agagtctaga gctgagaaaccaccaggcac cagtcatgtg ccccatctgt 89160 ctttctgggt ccatgtcatc tcttcactcactgcctgcct gatgcatcct cttctctgca 89220 aaccagcttt ctctgcttat tgatggtttctgctttctca caacctcagc ttgcctatgt 89280 agcatgaact gactctgtaa gagtatcacacccaccaaca actgactatg tcctttagct 89340 ctctatattc ttttgcttaa attttcaagcaagataacct ggttggtcac tggccaggtt 89400 gatggactgc ctgtgtcagg ttcaagtgtccaccactggt ccaaacatct gaactgaggg 89460 tcacatggta gaaacacaac catccaagcccaaggttttg gcagggctgt gggtaaggtg 89520 atttcccaaa caagaaggta tcaacagaacagggattcaa aaatatcata tttttgaact 89580 ataattacta tgaagtacgt aaccatcagaaggaacctgc cacttaattt agttctatag 89640 tcaactacaa gattaatgat ggaagtgtttccaaacttca ctgatctcat taaatctggg 89700 tactcactga agagttattt gagatataaatgtagaacta gttgtcagaa aatacctacc 89760 tgtgcataga aaaataaagg cactccagttaggctacaga acagcactat cgagtctgaa 89820 tgctcacaat aggctcacat cacttgctatagctcttttg gattaaacac atctaaaaat 89880 caagggggaa aaataattgg tgttcattttaatgtctgta tcaatggcgc taaggattca 89940 taggagaaaa gggcttgttc ttccttcctctcctagctcc ttccctccct tttccattct 90000 cccaccttcc tctcttacac tgaggctaccccaatcatat aaaggcaagt ccggcaggta 90060 gactgctctt aagaagccaa accaaggacaaagggtcctg ggggccaggg acaatctggc 90120 atatttttca ccagaatagt cagaggctgtctggccagaa gagaatctga actctagtca 90180 aaaaggaagc aaatatcatt gccagagaaactatgtaaac caccatcaag agtgagggtt 90240 gaggcaaggc tgtaaacagg aaagtgagtcagctcccagg tcagcagcca ggaggctcag 90300 tggacagaat aatactgagt gtgaagtaggtcacggcaaa cagctctgaa gcgaggtcat 90360 gaacgttgtg gtggtgtggg aagccctttttatgtggggc ctggcatggc aaagtcaccg 90420 tgcagaatca gaccccaccc atgcctaggctacccatctt ccccaaacag ccgggtgagg 90480 cagggcttgc cttatgggtt tgcctggtgagaggctgctg tggccctggg cattctgtga 90540 tggctcgtct cccgtggccc aggtgcccgggctggctcca tggcttcaag gatccaagct 90600 ttctgtttct atgtaccaga atgagagaggtgggctgcca gcgggacccc agtgtgtaag 90660 ttagggatga gcatggatcg agggagctagaagcaggagg taggattctc aagtcctact 90720 atttgaaaaa gggctagaaa catggcttgccggggacata agcagaaaag tgtggaggtg 90780 ctcggcagga gagccagact gtgctttctaacagggtgac tttgctttct aacagggtga 90840 ctggtgaaaa tggactctta tgttttcattttgtgggaca attaccattt gaggattgcc 90900 aacagtagct gttttctagc agtagagacagcaagtccac ttggatcaga tgccctttgt 90960 tctgagcaag tcgccggaat ggccctgttatcacatgtct atcacttcct ttcccgaaac 91020 aagtctgggg cccagctcta cagctggagggggcctggca cctctgcccc aaactcccag 91080 ctaaaaaagc ctggccagga gatgaggtgcacagatttgc ataaagagcc taaaaagggg 91140 cccctgattg agtagttctc atgctccgagggaactggaa cctactccac cccctactcc 91200 gggaagtaga tccagtttgt tactttagagcagctagctc ccactgcttg gcaagactgt 91260 ttattggcta ttattagtta tgagtatcatttgcatgggt gaaggccttc tgtactctca 91320 gtttgatgat gcctattttt actgtgtagagataagttgg gtcaaggaag gttgtgatgg 91380 ggtcagtagt caaggaggcc attagctatttaaatacctt aacctccaat ggatccagat 91440 taaacctcac cctcttgcag gaaatcagctgatagatgag tcacattgga gaaatctggt 91500 ataaatgaaa cctaaagaac ttatgtatgcccacaggagt ctctagaact ttaaatactt 91560 tgcttgatat catctaaact tcacaattgaagagaaggta tcctcagaac tctgtctctg 91620 ggatcttcaa catgtgtctt ctgtgttgagctggcctgaa gatggaggtg ctgtgtctgt 91680 aaaggatctt cccaaacagg ggttcatttaaggccaggtc aggggagtgt atccaaaacc 91740 ctccaccttg tactggtgct gaaagaaggtggagatgtgg catggcctgg aaggtagtac 91800 ctggccttgc acagtctgcc cgtaggtcagcgatatggtt tggctctgtg tccccaccca 91860 aatcttatct caaattgtaa tcctcatgtgctgagggagg gatctagtgg gaggtgattg 91920 gaacacaggg gtggtttccc ccatgctgttcttgtgatag tgagaaagtt ctcacaagat 91980 ctgatggttt taaaagtgac agtttcccccgtgtgctctc tctcttgcct gctgccatgt 92040 aagatgtgcc tctgccttcc gccatgatcctaagtttcct aaggcctccc cagccattcg 92100 gaactgggag tcaattaaac ctcttttctttataaattac gtagtatttt tacagcagtg 92160 tgaaaatgga tgaatacagt tagacaaggcttcaggtacc aggacttctg ctttccagag 92220 acatgagccc ttaatacttg atcagtgccacgatcaccag cagctcctct gcctcttatt 92280 gtgagaagca tctcactagt gtagcactatctggaccctt cccccctcac cccaatcaca 92340 gagaacactc agctaggccc cacccatataagagagaccc ctcaaaaaga tgctcaaccc 92400 tgggaactgg aaaagaaaac tgagtatttccttacatgcc caagaagttt cccagcagca 92460 tacacaagtc aaacggccag gctgcactgacattacactg ctcaacgtga ctggattcag 92520 attacccatc atccagacct gtgcacttgcctgctgccgg gctgagcact tgcctgctgc 92580 caggctgagc agatctaaca tggcctgtgctgctctcaca ggtgtagacc tggatgtcac 92640 ttctgatgcc actgccatgg cccctcaagtcacccatggg tacagcaagc ccctcttggc 92700 tccttcccag gatttcttgt ctattgctgatgaggtgtga gttgctaata aggtgtggga 92760 tgatcagctg gccacttcta ggcttgtacaaaccagaagt gcaagagaat tcatgcccct 92820 tggggcagac tttgaccaag gggagagggaaggcagtgaa ggagttactt tcccccagaa 92880 agactccctg aagtgcagtc acccacatggcagctctctg catatgtccc acaagtcacc 92940 cacatggtat ctctctgcat atgtcccacaagtcacccac atggcatctc tctgcatgtg 93000 tcccacaagt cacccacatg gcatctctctgcatatgtcc cacaagatca atcagctgta 93060 cttgctagca agcagtagtc aggtcaatgatacagctctt atatttactc tctttctttg 93120 cttcacttct cttgttcccc acttctgtttctctggtgat gctttcacta atgaagcagc 93180 agcaatatac ttttgcctca ggccccactgctccacacta agacacaata agcaacagct 93240 tccaagtcag ggctgggcag ggctctgaagggcaactcga accaaaccaa acagcgtggg 93300 cctggaagca cagggttagc agctccaaccagcctctgca gactgtcaac aaggctcaca 93360 tgctggcact ggctgggtgc aggtactgcagggttatggc caacactgtc aacgccatag 93420 gagataggag gcaaagtgat gcttcaggatccttggggct catggattaa taggtggttt 93480 ctactggcat atctgggacg agagcagcagagtactgtcc aagaaggcaa gagtcaaagg 93540 agaaaatcct ttggaggcct aggatttacctagggcagcc agaggcagca ccactagagg 93600 gagaatatga attggagcca gggagagaaagccaaggtca cagcttaggt tggagtgtcg 93660 agataaacag aaagaatgac aagcatgaggtataaaagag gtcacggcat gttgttcagt 93720 gccaatgcat gtccactgcg aagacgtaggaggcctgctt tcattctgga tgggcagagt 93780 gtaagaccag gccagggggc taaaaggagtgaggcaggag aagaccctaa aagccactga 93840 ggaatcaaaa tgaggtgcga ggtgagccatgcacatgtgt gacattcctt cacatcttag 93900 acgcatctcc agcaccctat ctttaagttatttcctcgtg gcattttata ctccatttgg 93960 gaaagcctct caagttaagt gttccttcagcttgcaaact ctctctctga aagcaataga 94020 aagccccaga gggagaggaa atacccttaccaacagcaga agttagcagg gggaggctga 94080 catttctaac tttagattcc ggctctctcattccccagct gtttggtatt gggcaaatca 94140 cttaaccttt caaagtctca gatttctagtcagtagagta aaaatactaa caactagctg 94200 actccaaaca actagttggg aggaatactaacaagcaaca atttgcaata gcatgaaata 94260 cctgcgtgaa gctgcgagga ccatgcctgatgcagacgag gcaggagctc aatcaatgct 94320 ggaacatcaa tagcaaatct cagcttccgtctggaagctg gcagcagcag cagcagccag 94380 atggcctctc ttggaagctg tacactgaagcctgtacttt ccaagttcaa ctggccccta 94440 cttttgaaga tctctcttgg ataccaggaatggagcttta tggacgatct cacaactagc 94500 aattcacaat gagtaagctg tcatgtgtactcttgtagat tcctggcgct ggggaggggg 94560 tgctacagag gccatgagat gctgggagagggcacccaag aggtaaagat tggcatgtga 94620 ttctctatca tctatcatcg ccctcggaaggtttacactt caaggctgac actattaagt 94680 cttcagcatc agaaagtact gtatgcagtaaccacaaagt aaccagaaag gggctggctt 94740 ctagatactt ttacatttgg ttaaaataatcccagtcagg gcaccaggtg agtctcttca 94800 agcgcagggc tgaagctaag ccaggttcatcccatgttcc acgcacaggg gcacgtgccc 94860 cattagtcag tttttctctt actcctcaggctgcccctca gccctctctg cctgcgcaca 94920 ccctcctcag gaagcaaact ctatttcaaatgtttactca tttcctgttg ccctcacatc 94980 aaatcctgag gtgtgcttcc tcactactgtccctgagtct aagcaatcta ggtcaatgta 95040 caactctctc aaggaacaaa ctcacattttataggagcct cgttgaatca gaatgactta 95100 acctgaagag gtgattctaa gaatagaatccaagccccat agctggatga tagaaagata 95160 tttgaaagat atcactttga gcaccctcaaatcaggccac actgggcaaa gttaggccta 95220 gtctggttcg acaaatggtc agtcctgacaaatttcatca gatctgattt tcctcttggg 95280 tataaatcta gtgccataca gttaactccacatttaactt gttaatcaca gagagctttt 95340 tctggtcaca aatgggtgac ttgtagcatttcccagggtt gagcaagtgc tcctgaagct 95400 ctgccctgtc tagacacgta tctgaaatatctaattaccc ctgtacattc tgtagagcac 95460 acaaagccca acgtcattac ataaacatgaagctccatat taatatattt gctcagtgaa 95520 ctgtcctttt acatttgcca atctgaacgtgaaatcatgc tactgctgag aataaggata 95580 ctatattgtg aataagaagt tccaagttgtgctatgatcc aaattatgaa agttaaatta 95640 tctagagagc aaaccaaaaa agctgtgtagtgaactgaat ccaaattcag aagaatctaa 95700 atagcttgat gcataccctc tcttaaatgacacatttaaa tagtttccct ttcacacaga 95760 gcaagaaact ttaagaaaga gatgaatccgtccacctccc tccctgtcag cgggtccctt 95820 attaggtggg tatccagacc agcccaggtcattagtcagt tgcactgggg gtggggcatg 95880 gagggaacag ggctgatagc cctggaccatctactatgca ccacatacag tatttattcc 95940 atttacctct tccaactacc tagtggggaaaattggaaaa tcattctcca cattgaacag 96000 ataaggaaac taagcctcag agaaattgaatggtccaggg cccagaacta ataaaaaaaa 96060 tgaagccaga ctataaagtt agggccttttcacactaatt gtcttcagac agatgcttac 96120 aagagttagt taaactcctt tttcgagggggagcacctgg cagcagcatg gtaccataac 96180 atgccccctc cacactctat ccggcatctgaaaatgtgat accacagagc agcctagtaa 96240 ggggagcccc tctcaactca taaatctgaggcaagtgtat aaacaagaat ccctcaccag 96300 tgggtggcgg ctgccctctg tgtctcccaaacctggggca tcaggctgtg ccatcccagt 96360 accctctgca tctactttcc ctgctggatgtaagctcctc gggctggcac caggtcctcc 96420 tcatctctgt gttcccatac ctagcacagagccttggtat aagatgcaca catggcaaag 96480 ctttgttgac tacatgatgc tttctttgggtgctcctcca ccctctgcag tgacatcgca 96540 cccctaaaaa ctcagtgctc ttagtctgtccccttactga ccagtggtcc taggccatgg 96600 cccatcctag gcccaactcc tcttctctccccgatgtctt gttccttcca gagcgcgcac 96660 tccccacatg actttgtatg acaatggtttaagcaattaa tgaacctttg tttatgattt 96720 gggcacagca gatagcaatg ctttaactcctggagagctc cccaatgtga gaaacctcag 96780 atcccagcaa gggcagacag acagacaaactggttgaaag gaagggtgct gaaagtattc 96840 tccatatcac cctgtatagg caatgactcaccccacctac aggcagaggc gaagtaacga 96900 agacacctct tcccagactt catgtccactcaccatggaa aaaatgcttc agatttgcaa 96960 aaatgcccaa gcgtaataaa aagcctgcattctgggccta ctgctgtgat attcactaca 97020 agtctgacat agaggtttaa tttggtctgctgtgaaaata gaactgaata ataatcacag 97080 tctttgattg tggttaggtt taattatagaaacctattca agtacatata tattaataga 97140 aagcctcaca aacatggcag aaaaccatttctgttcataa gtatttacag ggaaagctct 97200 ccttatttac tttatttaaa taaatatttcaaatcagcaa accaaaaaag agcccccatc 97260 tttcatgtga acattttcta atcatgtcatgttaatattt tctcctttga tttccaatga 97320 gctagcacaa gctctggcct ctgcactagatggtggtgct gagtcactta tttagaatgg 97380 aattatggat tatataaaga ctgacgagtacaatagatgt tcaatgtggt ggttgtcgga 97440 aacatcacct aattaaatat gctaaaatattttggctgaa aatttgtttt aaatcttcaa 97500 atttttatac cttctgattt tctttgttgttgttgttgtt gagacagggt ccgactctgt 97560 cacccaggct ggagtgcagt ggctcaatcttggctcacta caatctccac ctgctgggct 97620 caagttattc tcccacctag cctcccaagtagctgggact acagatacgc accaccatgc 97680 ccggctaatt ttttaatttt tttgtagagatgaggttttg ccatgttgcc caagctggtc 97740 ttgaactcct gagctcaagc aatcctcccacctcagcctc tccaaagtgc tgggattaca 97800 ggcgtgagcc actgcgcccg gcaacttctgattttgtaat atagaaataa ctgtcttggc 97860 caggcatggt ggctcaagcc tgtgatcccagcactttggg aggccgagga gggtggatca 97920 cgaggtcagg agatcgagac catcctggtcaacatggtgc aactctgtgt ctactaaaaa 97980 tacaaaactt agctgggcat ggtgacgtgtgcctgtaatc ccagctactc aggagtctga 98040 ggcaggagaa tcgcttgaac cagggagtcagaggttgcag tgagccgaga tcggccactg 98100 cactccaacc tggtgacaga gtgagactctgtctcaaaaa aaaataaata aataactgtc 98160 tttggaagtc agacaaaata atgagcagcctttatttcct tttaatctaa tttcaaatta 98220 atgttttaac ttcaagtagg cagggcaaaaacacaatttc tctcaaatcg ggtaataaga 98280 attaattgaa gggtgatagg tcgaaatacaaggagcataa aaatgaaaac acagagatat 98340 ggactcagaa ttgtacttcc ctttgctagacataaatttc actcctataa ttatcatttt 98400 taatgtccaa aatcttcccc acataggctatacttctttt tttttttttt ttttttgaga 98460 tggaatcttg ctatcgccag gctggagtgcagtggtgcga tctcggctca ctacaacctc 98520 cccctcccgg gttcaaccat tctcctacctcagcatccca agtagctggg actataggca 98580 cgcaccattg cgtccagcta atttttgtatttttagtaga gacggggttt caccatgttg 98640 gccaggatgg tctcgatctc ctcacctcgcaatctgcctg cctctgcctc ccaaagtgct 98700 gggattacag gtgtgagcca ctgcactcggcctggctata ctttttaaat gtccaaacaa 98760 ttaattaact taatctagtt ttttttattcgtaaaatgca tttcttaact gaattcaaca 98820 gttaattaag ccaggtgact tcttcatgactgacccgtta aaatcaattt tgcatttata 98880 aattaccatg aacctattaa tgcacagtataattactgta ttataactaa cttctacact 98940 aaaacttgca atatggactg tgaagccagtggagaggaaa atctttagac gtagttaatt 99000 gaataaatat ataaatttga agattcattagggatttaaa cattccgttt caaaactgaa 99060 gccatcacat actattattc taatttatttcaacctatag ttcatattta agtgctaaat 99120 attaaaatgt tgtaatagac gctgattatatcatcttcat gtaaaatgaa acaccagact 99180 ttgctgtcat acacactgga caataggtgaaaaaactcca acagcagagt aaaccaacaa 99240 cattcctaaa cgatctaaag attctcacagcctccaaatt tgcagataca gaaacttaca 99300 tataggccct cagttcttcc acaagctccaccacccatct accagcacct gtacccagtg 99360 ccccaccatc cctcatgtca ctaaacggactccctgtgct cctcttgatg gctgtcccct 99420 ctacctgcac acaagagacc accttgtcttgcctactcaa ggtcatcgct ttggcaatct 99480 ccccactctc ttttgcgtca ctgctttccccctctctact ggatttttcc catgagtata 99540 caaaaagctg ctattctcca atattaaggaaaagccctct tttgatcccg ttcctctctc 99600 tgcccaccac tgcatttctc ttactttctttacaataaaa tttcttaaaa taattggtct 99660 tactatcacc aaatccctct ccttctattgtctcttaaac tcaacccaaa cagggtttag 99720 tccaccactc cgttaaaact gctcttgtctatcacaccaa tgccctgcac atttctacag 99780 cagctgttca ttctcagtct ccatcttactggacctctca gcactgtata acacagctga 99840 acactcttca tctggcaaca cctggatagagcatattata catttcataa ctcagcccta 99900 tttgtcaatg taactttcta ctttgatttatccagcaatt tcattttttg gccaagaact 99960 gcaactttct caaaccattt tcccctttcttcattgtctt caccacacta gcatgtggcc 100020 tgtttagagc tacttctcag aacaaaataaagtatctaaa acacagtgat cattactgtg 100080 aaaatggtga ggcatagaga atgaagcttcactgtcatgc ggacgccatg tctcctttcc 100140 actagtacat gtgcatctta caccaagacatcagtggtac ctaatgtatc ttactcccag 100200 gatcctatga gcacaatgtc acaatatattggactaacat ggtgccctgt gtgaaacctt 100260 gcagacaaaa tggtaacaca gagccaaaaagaagatagag gcctaaaagc aaggcaatga 100320 agttattctg ctgcccctat gaggtacaagcaaactgttt attctctgct tatgtgcctt 100380 tagtaaaaaa agcatttggg ctgggtgcggtagctcacgc ctgtaatccc agcactttgg 100440 gaagccgagg cgggcggatc acaaggtcagaagattgaaa ccatcctggc caacatggtg 100500 aaaccccctc tctactaaaa atacaaaaattagctggagc atggtggtgc atgcctataa 100560 tcccagctac ttgggaggct gaggcaggagaatcgcttga accagggagt cggaggttgc 100620 agtgagctga gatcatgcca ctgcactccatcctggcgac agagagagac tctgtctcaa 100680 aaaaaaaaaa aatccatttg ccagatagatacatgcatac caaagatcaa ggcatgtatt 100740 gagttactag aaactttatc ctgaagaggacacatgttcc agtaatctct ccatatttcc 100800 atttccccaa ttactctagt aagtacactctttcaaaggg ccggatctag caagttcttg 100860 accttagttc catgtaaggg atgttgggcttatgaactga cctgtctggg aacaatcgga 100920 tgggggctat gatttcctat tgtaatagcttaaatcaagc ctccatttgc cagaagaccc 100980 agagtttttt ggttgtaaaa atcaggtggcatagctaagc acctatgcat ttcagtcaca 101040 ggaatcccat tatcagtagc cattcccaaagattcctggt catcctgatt aaatacccat 101100 cctgctgcct attatggaag ccatgctgcctacccacctt gctttctgat ggtaaggtgc 101160 tgctgcttaa gctctattac cccaggatcctaatatccct agtaagatta gggagtccca 101220 tttcaacggt accatacttc acaagcatcagaggtctaca gagaacagcc aaattttttt 101280 tttccaatga agccagttgg tccctcctcactgtatctct taggctcttg ttgaagagca 101340 tctcatcagg accctctcaa ggaaaacaattggaatacag ctctccatac ccatgatata 101400 tcccctccaa caatattatc tccctaagtgcttggacttc ttgctcttat agtagtacgt 101460 gacagaattt ctagcagctc attggtgccacagtcccagt tttggtcaac cagtcaagaa 101520 aacataaata gatcaatgcc atctatttatgctagtacac tgaattcaga acctctggtt 101580 aagtgctctt atagcaataa attcaagccatctaaagtgg tatttttctc tatttgacct 101640 aaaactttca aaatccatta tcatacatattcccccagaa tattttccag tatcaattgg 101700 gaaagtcttg caacttttta gcaccataagctatcacctc ctacctttga ctttgtcatt 101760 ggtgtcttga ggcattctgg gatctaagtttagttacaca tctgggagta gggcagaagg 101820 agaactgacc tacctttgta atgcaactccttcagatggc agaaaattcc atcaagacac 101880 gaatagcctc atcagagaag gtcacagggctgctctaact ggcaagggtg gattgatgga 101940 ctttagggat tcggggtgcc ctgaattcaccaaatcctac catatcccct gttctagttc 102000 ccagagtccc actctttctc taaatacccaaaccaaatac caaaataagg gacctagctg 102060 tgaattcaaa tgacatcctt agttgacaacttgcattact gcatttggct tttgactttt 102120 taaccctgaa agagttacaa aataaatttgtaatatttat atatagtaca aaagagattc 102180 ttttagtgta ctcgtagaaa gtccctatttttcaatccat ccttaggtaa gaatatttag 102240 gaatctgagc ttatattccc tttctttaagctgtctgcca ccgttacatg cagccatcac 102300 accagcccag tttttcaagt cctcattcctatgcaattgt tgcatggaag tccctgcttg 102360 gttcccaaaa ggttttcttt caattagcaactcattctag aagttcacag atgatttatt 102420 tgctaatttt ccaccaaatg ttatggggtgtcagtcttat cccttatgca gctggatttt 102480 tcactacctt cagctccaac taacccagacaaaccaatcc cagcttcccc caatttttct 102540 gtggttctat ttcccggaac cattcctggtttcaaactcc ctatcaatct aggttcttac 102600 ttgcaaataa cagaaataac aaaactaagactggctgctt taagcagaaa ctgaattaat 102660 tgaaacgatg gtggatattc ccacataatcattgagaagg ctatggaagg tactggaaaa 102720 caatcaggaa caacagtagg ctacccaatcacaaccaaaa tcatgccaca gaatttaagg 102780 ctgctccttc cactggacca ctcagctagctagctgagct aactacacca acatcagaag 102840 cagagacagg atgcgactac tggaactgccaccactgcca cttcagtaaa ctgatgtttc 102900 tatcatcact gtacacattg agtagaccatcacccattct ctgtagtctg tccattagta 102960 ccgagtgggt attcctgata ggctaggtctagcacatgtc ctgtagccaa ggtggctgtg 103020 atactgaata gtggaacttc ctagattcaatgatgagagg caagctctga atcctgctaa 103080 gattcacaaa ctggggaact tcccaaacaagggaagagaa atcatatgta aggcaaccag 103140 gaaaaaaaaa aagacaaata cccctgtaggtatcttcttg gtattttggg gaagcatcat 103200 tacagaaaaa gaagatgaat ttttatgtgagccaaagttg gaaaattttc atgctaaggg 103260 aaaaacagaa tgttgcaaaa gtttcatttatttgtaggtg ttttgcatct atggcagtta 103320 gtgatatttt atagtgaata aaaagaaagcaagtaaagag aaaagactcc cttgagtttc 103380 accatgcaaa ttagagaatc tggaaataatactcttgatc cctcattctt ttttctgccc 103440 tcaataaggc tgattcagaa aaagtatttataatatctgt taggatttcc caagaatatt 103500 ttaaaattct acaaggactg acaagagaacatttgctacc aattagaatg gatttgagca 103560 tcaaagaaca gaaaaattct tataataacctgatttcaca cttcccgtta agcatgttta 103620 gagtacatta tagcaaagca tgttaaagcaatctgccctc caaaaagtgc aactaagagg 103680 agagccaatg gagcaacact ggagaaccagaaattcaatt ctaggagaat gacaggagat 103740 atgtgagtgg tcagaggaat tagtgagtactcagtgattt tagacgcttt gccattggtg 103800 aggagcaggg aagttctttc tccttgttaaaaaagaggat ctggaagcta tagaaacaca 103860 cggcgatttc tcatcttaag caggtatgtagtcttctctc ttgctacaat cttcagagta 103920 ttatataagt attttaattt atccatttgttaacatttgg atatatgcat ccatttcact 103980 gtgtgtgttt tgacaacatc tagaagacaaataacttaac aaaatgtgct tagatgggcc 104040 ataaggaaga gtgaaaaaga agaaaggggaaaagaaggta ataacaagga ggcaaacagg 104100 gaaccaaaga gactatacta ttttcctatttcatgcacat gtgggtgcgt gtgtgggtgc 104160 atgtgtgtgt gcgcgtgtgt gtgtgcatgtgtgtgtgtgg tggggggtgg ttttgcctag 104220 caaatttgct ccatttcaat agaaactttaaacttcaaaa ttcctttttt tcttattgct 104280 aagtataaaa gctaatactt atttaagacccagtgcttta tattaagaaa aatataaagg 104340 tatacattta gatttgaaat gtagtctatgatatcggaga tatatcatga aatcagaagt 104400 aatactaaaa gcaataaaag aaaacaaagagaaaaaagag caaatggctt ttacatagag 104460 gttaaaaatt tactgaattc tcttggttcccaactaacac caaagaccaa agttcaccat 104520 tttgcttttt tctgaggcta cagagatagtggtcattgcc aaagctagaa ggcctgtggg 104580 gagtccagag gaatttaaga gaaatgactgagcactggag gtgatgaagc agaagcagca 104640 ttttccttgt tcctctgact accttttttttcaagcgttt ttaacatggc tcttgtaggt 104700 cttctttgca agcccttgcc agcaagtgaaaagcaatcta ggaaaccaca agcttggtct 104760 gtaattgaaa caaacaattt tagttcaaggcgataaaagc agtaacaacg aaaatggatt 104820 cgaagttggt atacaaaact ccacaattatgtttctttaa aaggctacag tcaggaacta 104880 catacaatat attttgtata ggaactctgcaacacaagtg aatatgctag ggtttctcat 104940 gagtcctgtg actctcagaa accttctgtcttccaaagag ttctttggat tctaccataa 105000 agtatacagc agtatatgga gagcatttttactgtttaaa atcacaaatg caaataagaa 105060 catactgtaa agggttgagt atccagtagcacactctgga tacccaaaaa tgtacaacag 105120 ggaagaacgg ggactatttt ttcatataaattcttaacaa tctgtaaaag gaaaaaaatg 105180 aaagcccaca aataagcaga gtaacttagttataaaagtt tagtcatttc aaattcatct 105240 caaaacaaca aatatagtat tttattcccactagactgta agtctcattt gtacaagagc 105300 caagtttgtc tttgtattat tgtaatcctagcacctaaca gggtacccag caaagagaag 105360 atgctcagta aatatttgtt gatgaaataaataaatactt aacgtgtaaa acaataatta 105420 aatgaatact tgtgttaacc ccatgtatgtacataacata cactaaaaca cgcttaggac 105480 cccaaataat gtcaattaac agggagaggctgaaaggtga gaaaagagga gagatgaaag 105540 cataaaatag acagagaatt gtcacttaatctctggaaat tcctgtaagc agttatccat 105600 tgttcatgac accttgaaaa ttctcatctttaacctatta gaatttcttc aactggaata 105660 gccacagcag cctccagaga catcattttctctgggcata attagtggtc tcctccttgc 105720 tccattatcc tgataacatt caatgaggtctcatctggct gcattatgtt tgggacctgt 105780 ggtaatgtca cagaatcaga aagccttacacatgaaccat ctaattcaac ctccttaatt 105840 cactgatgag taatttccca gaggttctgagtgccttgcc ccgggtcgca gcggaaagaa 105900 aacttctttt gttccacaga gttctggggtacgtgataaa agaagaactt cagctgaatt 105960 aaatttaaag gagtttgagc aatgaataattcgcgaattg ggcagccccc agaatcacag 106020 cagattcaca aagactccag tgcaaccacgtggtagaaga agatttacag acaaaaaaaa 106080 aaaaaaaatg gaaatgacat acagaaattggaaggagagt acagaactgc cggttggtta 106140 caggtcagtg tatatgcctt atttgaacatcgtttgaaca ttgagcagtg tatgaaaggt 106200 tgaaatctgg cctctggcca ctgggattggccaagacttg gctattgtta caggtgcata 106260 ctactaaatt aggttttcaa tctcatctgcctttaagcta ggttacagtt caccacaagc 106320 actcaaatat aaaagtacag agtccttctcaggccatatt tagtttgctt taacatactg 106380 caaagaatac taacttatac atcccaaaaggctcatggac agaaatgatt tgttactgaa 106440 tataaagatc tattagacac ctggaaatctttggagacaa agatgcttca aatagccata 106500 gaagggaatg cataaggaag ctaacacattacaaatacaa aatttttcta tgcctaattc 106560 aaactgaaaa aaaaaatgtt tttttaattcctgtcattct ccagagagag gaaagcaaaa 106620 attgttctct acatctgagc tgtggtgtcagcatattacc caccactaga gcacaaaagc 106680 aaagttcagg ccctcatttt agaatcaacatgggtggctg gtgaccgtca atttcaccaa 106740 tgggtaaaaa cacacgactt ccctggatgcctgtcatttg cctctcatcg catagtaacc 106800 agctgtataa gtaagcaaaa aaatcctcgaaactgtttat tgactataat taacagggtt 106860 tatggtattt gaaagcaaat aagcactatgacaaatataa taaaatatga tgcataaaaa 106920 tgatgtaaaa ccgacttgat gttctgactttccagtgaaa gtcgttttcc tggtttacct 106980 gatgaaatgt ttcagaggtt cattgcatgaactatgagtc atagcttcaa gtctctggtt 107040 gccactttac catttacatg atttaaggccaaaatctgct ctccttccta tgtcataaca 107100 aaaccgcgca agactataac gtaagcctcactacccatat cctagagctt tgcagcaacc 107160 agcaaacgta aaaacggaaa acaccagagctctaaacaga ttacccagct gctgtgtaaa 107220 tttacagcag tctataagcc ttcataatattagtaatatt tataaaatta ttgagcagtc 107280 cttgattata gtgtattata gaaatgcaaattgcagtcac aagaatagga tgtgggttgg 107340 gactcttgaa atcaaccttc attttttgacctgacatttg atattttgct ataaagcctc 107400 cagtttgaac gttctacttg tcaagagttaatgaaactga aatgagtcac agatactatc 107460 tatgactcat attattataa tatcacaattatataatact gcaagtattc atggtgcaaa 107520 aaagaatgcc ataggacaaa agaccttccctgtagatcca gtcatcacaa tgaataaagc 107580 cctaagcatg cttgctaatt ttaatgggaccgaagagtct taacgcaagt ttcccctctc 107640 ccacccctgt tttttattta attatttttaacctgcttct ggtccaagaa accatttcct 107700 agcctggagt gctaggcatt tactaatggcctttccagca gggctgacat acatgagtgg 107760 ctgtctccag ctcactcaca gacttgggggcggctcttag cagcctgttt ctacctgagg 107820 gtctttgtat ttaactgaat tctttagtgacagggatctg cagaataaag aatgctgcat 107880 ggggtaggaa tgcacacacg tgttttcagctgcttcatat tcaggcctgc tgtgactcag 107940 cttacaagat caaagttata aactcatttttcatttctta ggggaaaatg tctcagtaaa 108000 agtacgaaaa cctgaaacct tttttcaaggaagatacaaa acaaactttc aaagtttttt 108060 tgtttgtttt gttttgtttg tctgttttgctaagtagtgg tcagtatcac aagatgacat 108120 ccatgaagct ccactgggga ctctaacattatatataagt tgattcctat ggcaaagtgt 108180 ataaactcac tggttattgt cattgtgttcatccaccatc attgagcata tctctaaaac 108240 aatgttgtac tataattttt tcccaggagttttagagaga aaataaaatt cctctaatgg 108300 agacagtttc ttgacacaat tgttcagtcctcgaagcact tcattattag gtggtagcca 108360 tacatttgaa tcaataaatt cctattcagactctgccttc agagaaaatc ctgtagcagt 108420 ataattaatc ctttgcacct gaggttaaaaatgtggccga ataataacat catttttagg 108480 cactggcaga gcagaattaa gttcaataaaaagtgggaaa gataaaagga aaaaaaggaa 108540 gatccaacat atgaagtggc tgctattttatttcaacatt gcttatatgt ttaggaaaac 108600 atttaaaaga tgagtagaca agaatgaaatgatgtatcac aagaagtttg tttccttctt 108660 acggtttcaa aactgaaatt gttagagaaaatgaaaaatg agtttttaag tgttgagagt 108720 aattctagaa aatatttgtg cacacaaagcctgactagac ctattccaaa tgatgttctt 108780 atctggagtt tcccagctag gttaatgcgggcattaccaa atcctgaggt acctaaaagg 108840 agttgtcaga gaaaagataa aacaaaaataaaagatggag aaacaaataa tatagagtgt 108900 tttctgatca gaaataagcc tgccaactacccattagaga ctggttaata cttgtgagtc 108960 tcaaattgcc tgaatttatt ctggagcttctagatatatc tggggaaatt gagacaatgt 109020 acttctttct ttttgtaaaa aaacagtaggtgttacccaa ataagtggga acatcaagtc 109080 tggtctttat ttctgaggtt gcctgcaagttgctacatcc ataacaaaag tgcctttgca 109140 gtttactctt cagcatatat tatttcataagcaaggtact caaattgaat ctcagcttca 109200 aaacttagta tcaatcacat ttttcaaccttccagtttag ttttggtatg actggaaaat 109260 tctgcaatgc taaggaagtg tgccaaccataaattaaaat aacatctgaa tgtaccctac 109320 atagtttata agcctacaaa ggacctaacgtcatgaggtt tatgagtaac aaaccttggt 109380 gacagtaatt ttccttagct gaaactctctatcacttaaa atattctgtc agacacccag 109440 ttacactttg agatttctca tcaggcttttagtattttaa aaactcaatt tctgaaattt 109500 cgcctatagg ctcgatcctt ccaaacacacctccctcctg aaatttcatg ctcatcacag 109560 ctgaatgcct gcgactgtcc cgaacacttcccctcgctac aggaaaatgc attaacttcc 109620 agctgcccgg ccgagtgtgc catgtccgtatcaattaggc ttgagcattt aaaacggctg 109680 ttcctgaggt gattaatgac tgcaaatgagaagacctttc gtttcccggg gacaggtcac 109740 cccagaccct gtgttatcgc caccagtcaaggcatcctgc tgaaccaggc tgccatgcat 109800 cccctttcca gcccatttat cagtcaaacctgccaggatg attcttttca ggtgtgcatc 109860 tgagacacga ggggagagac ctttgttttaattgcccatg atttgcttta tggtctccaa 109920 gaactcatga gaacttctgc caaagtagtttcaaattcag aagctgggac aaatgagcat 109980 ctccaccatg gatgaggagc agtatctagatacacaatgt tcaaaagcac ttttcagcac 110040 tatgatttac aggcaagtac tgaaaaaaaaaaacttagcc aagaggattt tttgaatttg 110100 taactaaagg taaacctgtt ttatagctggcatctcattg ttcaggaaat tccaaggccg 110160 gctgccatca aaggttgaga aaataaaatgatttaattca agttttcaaa ttgctgatgt 110220 aaaatggtat cattccattt ctcatccacatataagtgag ctgaacaatc atcaggcagt 110280 tatagtcaaa agaaactttt cagaacaaagctgtcattaa gagatataaa aacatttatc 110340 caaagaagac ataacaatgg ccaacagctatgtgaaaaag tgctcaaaat cactaattat 110400 tagagaaatg caaatcaaaa ccacaatgagatatcacctc atgcctgtta gtataactgt 110460 tatcaaaaag acgagataac aagtattgatgagggcatgg agaaaagaga aactttctac 110520 attcttagtg gaaatgtaga ttggtacagccattgtggaa aacagtatga aggatcctaa 110580 aggaattaaa aatagaacta ctgatacagtggctgctgcc atcatgtcag ctgcagcagg 110640 gaggcgtggc tggggctgca cactccatggagccagtggg agccctgcct cttctgagtt 110700 ggaaagagag ctacgtgcct ctgcagccacccaaactgca gctacagacc caggcctcct 110760 gctctagtga gcaagcagga gcctcgccctcctgggtgag gctacaggcc acctaaacta 110820 cagctgtgaa tccaagcctc ccttgtgctcttgggggagc caggaacagt caggatctgc 110880 cctcctgggt gcagctggag ccacggcactggtggctgca gatctggaac tcccagttgg 110940 gaagcaggca ggagccgggg acaagcgggagccctgcctc ttccgagttg gccaggccgg 111000 agctcctggc tgcagctgct gctgccctcccacgtgcagg gcgtctctgc agcctgcacc 111060 ctcgggcacc ccaggaaggt tccctgcccctgtccctgaa ggctcagggg tgtctgctcc 111120 cactgcctgg cctctctccg ctcccagtccccagctccaa tctcggagcg gggttggggc 111180 cgagccccag ggccatgaac ggcagcaggaggcagacaga gtccggggtg gaagggggca 111240 gggtccccag taaggcccca ctttcaggccgtccctggcc tgaacactgg gggccgggct 111300 gccagtcctg tggacgggag aggagactcatggtgcctct ttggcttgcc catggaccaa 111360 ctggcatgca ctctgtcctc tctgaactccataaaagctc tgtactcagc cagagcaggg 111420 cagaggatgg ctaaggacga agagggcagagagaatggga gcagaccaca aggtgcagag 111480 aagactaccc tgtctgctga tagctggagacgatgggaag accagctgca gagaggagta 111540 ccctcactgc tgagagctgc agagatgacctgccagtaga gaggagctac tcactccttt 111600 gagctgttct aacactaaat aaaactctgcttcttcaccc ttcacttgtc tgtgtacctc 111660 atttttcctg gatgcaggac aagaactcgggcaaaggtgc tggggccaca gaggtttccc 111720 accagaaaaa tcgacaccca agagatctcctaacactact ggtttgggcc gtaatcccag 111780 cactctgaga agctgaggca ggaggattgcttgaggccag gagttcgaga caggcctgcg 111840 taacatagcg agacctcatc tctacaaaaaaaaaaaaaaa aaaaaaaaaa aaggctgcgt 111900 ttggtgatgt gtgcctgtag tcccagctaccctggagact gagatgggag gagtacttga 111960 gcccaggagt ttgagcttta cagtgagctatgattgcacc actgcattcc agcatgggat 112020 acagagtgac actgtgacac actgtctcttaaaaaaaaaa aaaaagtcta atggaaatga 112080 aatcaccact taacttcata aaggtatccacatttccttg ttcattgcag cattattcac 112140 aatagtcaag atactgaaac aactgaaatgtctgttgatg gatgaatgga tacagaaact 112200 gtgatataca cacatacaga gaaatattatttagccttaa aaaaggagat cctgccattt 112260 gccacacgaa tgaacatgga tgacattatgctaagtgaaa taaaccagac acagaaagaa 112320 aaatactgca ttatttcatt tatatatggaatctaaaaaa agaaaaacaa agttgaatac 112380 atgaagatag aataaagtgg ttgttaccaggggtgataga agtagttgtt accagggatg 112440 tggtgagact ggtaggaaat ggggagatatacataaaagg atacaaagtt gcagacatgt 112500 aagatgaaca agtctggagg tctaacatacaacatgagga ctatagttag taatactgcc 112560 ttttatttga gattcttgca aaaagagtagattttacgtg ttctttccac acatacgcaa 112620 agagggtaac tatgtgaaat gaatgtgttaatttgcttga ctataataac catttcacaa 112680 tgtatatcaa aacatcatgt tgtatacttcaaatatatat aattttaaaa aagtggtttt 112740 tttggtggtc aaaaaaaaga gatacaaaaacaaaagccct gtggatgcat catctggggt 112800 ttattccact tgacatatga gactctgccttatggtaggg ctcaggtatc cacctctgtc 112860 ccttctggcc ctgggcatgc caaacctacacccagaagag acttgtgcta ccctttccac 112920 atcttttcta ccccatgaca tcactggtatgttactcaga ttttggtgtt ttctaacagt 112980 ctcaggacat aggaaaaaaa tttacacttaaagcttcaga acagccattc aaaaaattat 113040 cacttaatag aatgaaaaca cacatgtaccatgaatagga gagggaaggc attaaaaaaa 113100 aaagacttct taaggtcaat tttccaaaaggtctatgttt aaatgttcta acaaaatcta 113160 ctgcataagg acaaatagat attaaactgttatattggga aaaatactgc atgacataga 113220 atgtctgaaa tccaaaaata aattaatatcactacgcttt gccctttaat caaaagtaag 113280 ttagtcactg gtatttaatg cacacaagaatctagtataa catggacaca tgaaggggaa 113340 tatcacactc tggggactgt ggtggggtggggggagcggg gagggatagc attgggagat 113400 atacctaagg ctagatgacg agttagtgggtgcagcgcac aagcatggca catgtataca 113460 tatgtaacta acctgcacaa tgtgcacatgtaccctaaaa cttaaagtat aataaaaaaa 113520 aaaagaaaaa aaaagaatct agtataaaattgctaccaaa aaaaaaaaaa agaaaaatta 113580 gagaaacttg agattgtact ggcagcttgaaattttctag aattctaaat tttaatttta 113640 gtctctacct taatataaga tatttgccaaatgtccatag aattgaaggt tttgtactac 113700 tcactaacaa tatataatgt ttgcttaacttgggtttgat gtagaccagt ggttccacaa 113760 actagatcat aaccttagaa ggtgagacatacgcatcgta agttcttaaa agttctccag 113820 gtgattctca tctgccgtgg ttgggaaccactgacacaga ccaatgcttt ccaagacccc 113880 accttgttag gaaaagaaaa cacaagtatagcagttaaga acccaggagt aactcagaag 113940 aagtaagtta aacagatttt taaaattatagtgatgtaaa taagatacta acattggtgc 114000 ccaaaagtaa aggtaggttg gaaatggtgaaatgtgtgat gtaattggta gtttggggcc 114060 tatcagggag acttcttggg aaagtcttaattataaactg aattataaag gtcaagagtc 114120 agtaaatcac agaataaaga ggcaggcattttaaagatga ccccttcaaa taatctgagc 114180 attcaagcca ccaagtaata atgactataattatcatgtg ttctatcttt attttatatc 114240 attatttaaa aaggaaagct atttcagactgggatttctt gtgcaaagtt tttttttttt 114300 atttgggttt tttgtaactt atggaaaatatttcacccaa aaaggaatgg aaatctttat 114360 ccagcctaag agtgagtgga agagaactaagcaaacggaa acaaccaata gctcaaaagc 114420 tgagtaccat aaacacctac acatttgactggacacgatt tcagtcttct ttaagaggag 114480 ggacttaaga gtcttaagag ttttcagctggaataagatg tataaggata aattatgttt 114540 attcacataa actgttttgc aatatgaaaagaccaaaaac acctgtaaag ccagtgtggc 114600 tcccactcca ggaactctag ctggtaatgtttcctgacag agtaatttat cctaaatttc 114660 acatcctgca agcataaggg tcaaatatattttagtcata aagacactat aagtttccga 114720 gtagaaatgc ttcaagtttt caatctccaagaacatttgt cccacctatt ctgataatac 114780 tttactatcc ctttgttttt caagataccagaaatgactg agatggaatt tactctagct 114840 tacttcttat atttaaaaag atctcttgcttttgcattta tctaggcaca tataaaaatc 114900 aatatgtgac ctactactac ccagacagaggttagcatca ttttctaagg ctccttgctc 114960 ttcactgtac tttggtcttt tccatccctgcctgactcta caccactctc aggctattct 115020 tcccagacac tttttctact ctgccgtcgtcctctgccca ggaaccaaca aagcgttccc 115080 tgtcggatca agtcctgaac actcagcttgaccctctcta atcagaagaa tctgcctctt 115140 caactttatt tccacgttcc ccaaccttttcattctaatt aagccaatat cccgattgcc 115200 ttccatagaa atcatgtttt cactcacatacctactcagt aaacacttat tgagtgccag 115260 ctgagtgtca ggcctcgtgc tcatttctggccccacactt ttgttcctgc tggcctggct 115320 caaatgccaa gtcctccaca aagctctcttcagctgtgct gattccccac tcagcgtcct 115380 tatcctcggg attccccaca tcagcattcgcaacctgaac tcacacagca cttttctaga 115440 atactttcag acattctgtt tcaaataaatttggaaactc tggttcaaac tcagttggac 115500 aggtttctct actatggaat tctcagaggctttaataagc taccatcagt tgtgaattcc 115560 taagagggtt atataatatt cagaatttcacaaaattcct tggccataaa aattttacag 115620 tataattgct gaaatcccag gacaatcttatttgggaaat gttgcctact attatttaaa 115680 attggtacca cacaatttat cacttaatcacattgctggc tatatggtat catcctgtta 115740 cttctctgtc ttctttctat acatctttgtccttttcaaa tacccaaaat attaagtcct 115800 cccttatgac taaaagtaaa gagtaaaccaaaaaatactg tctcaaccct tggggaaagt 115860 tttctaatat gtatttattt acctccctgccttagtaagc agtacaatca atggatcttt 115920 gatggctgag acaggttgaa atggcaaaaagacaacagca ccacttttga aaacgacttt 115980 atgtagacca cctccctggc ctcagcgtcctcatctgaac aagaagggaa cttcccagtt 116040 ctgacggtct atcgtacagc atctcagcgttgtgattctt aaataagtat tcactgtaca 116100 atcctataaa ggtaagagct aaacaatcagatatcacccc cagaacattc ttcggaagtc 116160 tctgtgctct gctttcttac taatataataatagcttgtt ttcttatgat tttcctcctt 116220 cgaataaaaa caattcctat taatttaggtttcctgtcat ttggctttga gttaacacag 116280 gctttacttg acagggaagg gaatgactcggcaatcacag caacagccac acctgcagtg 116340 ctccttcgtt tcccaagtgc agccagtaactggtcagatg gagtcccaga gggcatgggc 116400 cacagagaga ggaagtcatc tgatgggagggtgggcatga acccatggac atgaacccat 116460 gaaaaacagc tgtcatgtgt ctccatatctagttatactt caggttctag tggatgaaca 116520 accaaccaac caatttgttt tataatgtagggtttcctaa gttaacaaat cttaaatttc 116580 ttaagcttcc agagcccact ggaaaaagatcatcacaatt ctaagactct ggaaatgatg 116640 aatgtcaaaa caatctctat tccattattccaataaattt tgtgacattg atgtgatttt 116700 cacacaatgt ttaatgcaaa atgacaagcaactcaaattt ccatattatt cttttaacca 116760 tctgtaaaaa atagataaat atgacatctggtgaaagctc taatgaatga aaagtgcatt 116820 atttcctcct cacagcaggt gtgtgactttgggcaagtta cttctctaag cctcagtttt 116880 ctcatctgta aaatggggct tatttcatagagttgtcctg ggtgttcaaa aaaataatgt 116940 attcaaaaca cttaacacat cattgtgtgactgttattgc ttttttgctt atggttgttg 117000 ttactatgat tatcgtttga ctagtgttattgttataaaa aggacagtta gaaagaattc 117060 tattaaacct ctggagatta cctcacttcgatttgaaagc caattctttc tgcactgttc 117120 taagtcaacc tatacaaatt gttagatacaagttggaaaa gcccataaaa tcgtgtggtc 117180 tcttttgcct ttaatagatt cctcctttcaaccactacgt atctatctcc cccaattcac 117240 atcatcaaag aaagcttaat gcaattaaatggggaaagaa gttaagatga aattagacaa 117300 agccacatta aagcagtcag ctcaattcttgacacacaga ttggaggaca aataaagggg 117360 tgaatggaag agatgttttt cttcctaggcagcaggcata agttacactc ccataaatgt 117420 caataaccag ctgtgtgact ctgagtgggttgagaaacta ttagaataga tgacctccat 117480 agttcccttc cagctcagcc tcagagcccacactgttgct ttctgtaaag ttaagtctga 117540 aggggaggat cgggctaaat attaccattttaaaaaagaa tgctagtgct attcaagaat 117600 gaagtttaat tctcagtcct ttggaatacaacttcaactc atttacttag ataaatgctt 117660 tccagtacat ttattaagag agcaagaagtgatgttttga ttaaaagtga attctaaaca 117720 tttgctacat tcagagcagt actttgttagttatattttg aacttgcatc tgtatttata 117780 gaagcaagtt caacttatat atataggactaagagctgtt gaaataaaaa tttgctatag 117840 attttgcaca tttcgggtga ttaatcaaaagatttcatct acctaaacat ttctttccat 117900 tttttttctt aatcttggat cctacttatctaagcaggct tcctttctac tggatataat 117960 ttcaataact tcaaaagcaa acacaaagtaatcttatctg tgacatttct caaacacttt 118020 tttctaagtc tttcttattt catttgttatacacaaacct agatggtgta gcctactata 118080 cacctaggct atatggtatg gcctattgctcctaggctac aaacccgtac agcatgttac 118140 tgcattgatt actgtaggaa attacaacaaaatggtaaat atttgtatat ctaaacacag 118200 aaaaggtaca gttaaaaata tttttttaacggtatgcagg ctgaatacag tggctcatgc 118260 ctgtaatccc agcactttgg gaggttgaggtgggtggacc gcttgagctc atgagtttga 118320 gatcagccta agcaacatgg caaaactctgtctctacaaa aaatacaaaa attactcagg 118380 cgtggtggtg tgtgcctgta gtcccagctacttgggaggc tgaggtggaa ggatgtcttg 118440 agcctgggag gcagaggtta cagtgagtggagaccacgcc agtgcaccgt agcctgtgtg 118500 acggagccag actttgtctc aaaaaaaaaaaaaaaaaccc aaaaacgaaa aatggtatgc 118560 ctgattactg attagggcac ttaccatgaatggaacttgc tctgggtgag tggtgagtga 118620 aagtcaaggc ccaggacatt actatatactaatgtatatt ttataaacac tgtacactta 118680 ggccacacta aatttatttt ttaaatatttcttttttcaa tagtgaatta acctcagctt 118740 aacataactt ttttaccttc taaacttttaaattttttaa aactttttta ctgtcttggt 118800 aataacattt agtttaaaac acaaacacattagctgtaca aaaaaacttt ctttctttat 118860 atcctaattc aataaacttt tttccatttgatacagtttg gctctatgtt cctgcccaaa 118920 tttcatcttg tagctcccct aattcccacctgttgtggga gggaccgggt gggagataat 118980 tgaatcatgg ggtcaggtct ttcccatgatgttctcataa cagcgaatga gtctcaagag 119040 atctgatagc tttaaaaggg ggaatttccttgcacaagcc ctttctcttt gcttgctgcc 119100 atccacgtaa gatgagactt gctcctccttgccttctgcc atgactgtga ggcttcccca 119160 gccatgtgga actgtaagtc caattaaacctctttctttt gtaaattgcc cagtctcagg 119220 tatgccttca tcagcagcgt gaaacagactaatacactat ttttatttta ttttttttac 119280 ttttaaatgg tactgttaaa aactaaatagaaacacacac attagcctag gcctacacag 119340 ggtcagggtc accaacatca ctgtcttccactctacatct tgtcccactg gaaggtcttc 119400 aggggcagta acaagcctag agctgtcatctcctacgaca acaatgtctt cttctggaat 119460 tcctcctgaa ggaactgctc gaggttgctttgcagttaat ttattttttt aataagtaaa 119520 agactctaaa ataatgatta aaagtatagtataggaaatc cataaaccag taatatagtc 119580 atttattatc actgtcaagt acatactatgcataattgta tgtgccatac ttttatacga 119640 atgacagcac agtaggtttg tttacaccagcttcaccaca aacatgtgag taacctgttg 119700 tgctgtgatg ttacaacagc tacctagtcactaggtgata gaaacttttc atctccatta 119760 taatcttatg ggaccaccgt cgtatatgcagtacttcaat gaccaaaaca tccttatgtg 119820 atacctgtgt actaaatgtt tgaaatattgcaactcagca gaaattttaa atggatcatt 119880 tatagaaaaa ctgctagagc tctttgagagacaataaatc caattcagtc tgcccagtca 119940 gagcaaagac aaccccttca gaagttttaagaggaagcaa gagcatatat tctctcagat 120000 ataacacgta tattcgtttt tcagcagttgcaggactact ggctggctgg agctagttca 120060 caaggacaac cacatcagtc aggaagacctgtgacccaat ggctctcatg tgctcatgga 120120 ctaaacagga aacaaaattc tgcgaccccaagtatggtaa atgatgaatg gcaagaaagc 120180 aactggtcta tcttctgatc atgtaaattcctttccttta aagggaggtt tgtctgccca 120240 gaggcttaaa ggcaaatcat gaaatcacgtgtcttctgga aaaagacaca ccagctgata 120300 aaaacatggt ttctccaata atcagttaaaaacatcaaaa tatttataat tatgtgctat 120360 tgcttgagct ggcattctct atttctcatcactttcatca accaaagttt attagggtat 120420 ggaagttgat aaaacttaga gaaaaatcccaaggaaattc catgtctttt gtgttccctc 120480 aaactgaagt tttcccagta tctgttttctccttattggc aatggtgaga aggaatacta 120540 tatgaaaaga gatttaaaat ctatcatagactttgtcata tgagaacaat cctttctgga 120600 aaagaaatgt aaacttacag ggatttcttaagggtctcca tggatataca ccatccccct 120660 cctccagtag ctgttagctt ttatgtgcaaatgtactgaa ctagagacct attatatcaa 120720 aaggacattt tcatcctaga atatttttcagaaaaacaag cctcaaaatg taactcttct 120780 gtgcagatgc aatcacccaa tcaattcttctgctgattca acattctaat aagactaaca 120840 atccattcat tcattcattc attcatccagcaaacattta ttgaatgccc actacaaagt 120900 tcagtgttgt aacgactgag gctctgaaaatcatggctcc aaaactcaaa acctatgaaa 120960 ccttggataa gtttcttaaa ctctcaagtttcggtatcct tatttaaaat actagttgct 121020 ggtatgcagc tactttttat gaaccaacctagagaaaccc catcctcctt attcagatgc 121080 cctactggga ggaaggtgcg tgggtcaacctagaggaaga ctatgatata ttttgaaatg 121140 atcttaggat aggacacaga atctcaagcaggttgctctg gatctttcca ctgttccaag 121200 aacaaggatc actcaccaag gcactaaatgcaaccatttc tgttgcatac agacagagaa 121260 aattaataca tttaacaaat gattaaaagaagtgttcagg aagaattcaa tatagaatca 121320 cataactaga atggcaacta ttgattagatgaggctgaaa gggcagctgt gtgtatttca 121380 aagggctctg aatgccagat gaaaacatctgtacacagag aggggaagac atgaataggt 121440 ttgaacccta aaaagatgtt tgtcgacaccaagcagactg gactggggta gggagtgagt 121500 ggaggctgtg aagccaatta agtttgtggtcacagttgac ataagaatgt aaggaagaaa 121560 aaaggcttca atagcataat gctttaataccatgcttctc agccttgagt attgcatggg 121620 ccatttgtaa acaatttaca aagttaagttgttattatca tagtgttatt taaaggtgta 121680 caatattcct tatacctata gcgccaatgaaagtaaaacc aaaacgaagt tacaaaccca 121740 ggtaatattg ttctaaatct tgtcctctttctggcttttc gacacctcta ggtcttccaa 121800 cctccttcct ctaaaactaa cgattgtctgagagtgtggc acttcaagca aaagacctgg 121860 gttcacactc tgactctgcc acttactagctggagactta gggaaattgc ttgatcaata 121920 tgagcctcaa tttcttcatc tataaagtcagcagaataac ctaccccata cagtttttgt 121980 aagaatcaaa acaattatgt ggaaagcactctgttaccca tgagttctca ctaacacaat 122040 cttgtggact tgcattaata tgcaaaagacaacagaagtt tactaaagta agtgacattt 122100 tttggaaggg ttctgaacta ttaatattttaaaagagaaa aaaatcaaaa aatgtttata 122160 agaacaagac aatcatttat tcttaactactcaaactcag aggatcatgt gtgcctccat 122220 ttcatgccat catttgcaga ggaggaaaagtagacaggtt gagggcccgt gtgtgttgct 122280 gacaaaggca aactgagccc aggccttgtggctcccagat ctgttcacca attaaaagct 122340 acaaacttca ttgataatgc ttatagccagctaaaacagc accttctttg gaaagcagca 122400 cctgggaccc ttccacaaac tctctgcaatgtgctaacac aagtctcaaa gaccgggcat 122460 ttcccaggga agatggagga caccacacctcagaaccttt gctaccctct gagacaaggc 122520 tcactctcca tgtgagagac ctcacttaagtgaaaagagg gaagcagtaa tccagctcag 122580 gatcaataga aagaatcaac taagtcttcctgtgtagatc tatttcatac caaggacttc 122640 atgtcttcaa tttggaaaga agttcaaagaaaaaatcagg gacaagatta cacaatttac 122700 aaactgactt cacacacatc atatttgatccacatggtca ccatgctaga ttgatattat 122760 gacccaaaaa cactctccaa caaaaacaacatgtttattt ctattgtctt aatcttaagc 122820 aaaaaatttt acttgatttt tccgtttcttctgttcttgg agaaaaactt ggcacacctt 122880 taaagcagta gtgaacttcc catttttgaggaggcttaaa tattatttat aaaaatagca 122940 aataaaatgt taccccacca ttcctccttcttgaaattaa tatggtacag tgccaaattc 123000 cattcatcct ttcacttatt cataaaacaaacacattggt agcacccagg atatgccacc 123060 attgttttat cctggaggac ataaaaatgaaaacaaccaa agtctctaca ctctgatcat 123120 aaaatcaata ttaataaaaa attatagcccgcaatgtgga ctgatgtcaa aaatcatgaa 123180 ttcagggtaa aaagaaagtt actaaagactgggatgactg tacaaataca caggaaagaa 123240 gtcatcaaca aaaaattcta aaaatgcctcaaggtttggg ccattccttc gagaacagca 123300 tatatagtag ggcctgtgat ggatggccaatacaaaaacg ctctgacact aacttaattt 123360 gcaacttaac aagagtatta actgtgtacttttaacataa aataaaatgg gtgactatct 123420 ttccacaact tttaatattt attctgtgttttttagcttg tcctttaaaa aatgaagaat 123480 ccattaacat tagttttcag gttaccatcttaggcaacta aatatgaata atcaatgcaa 123540 attaaccaat attatataag gaaatttgacttatctttca acctaagcag acatgatttt 123600 tttttttttt tttttttgag atggagtctcgctgtcaccc aggctggagt atagtggcgc 123660 gatctcagct cactgcaacc gcttcctgggttcaagtaat tcttctgcct cagcctcctg 123720 agtagctggg attacaggtg cctgccaccatgccaagcta atttttgtat ttttagcaga 123780 gacgggtttt caccacgttg gccaggctggtctcaaactc ctgatctccg gtgatccgcc 123840 cacctcagcc tcccaaagtg ctgggattacaggcgtgagc caccgcaact ggcctaaaca 123900 gacatgataa tctatttctt taataaagataacttaagtt tgcctaaatt ctctataatt 123960 acaaaataag aatgttgtac taagataagatttttaagat agacatagca atacgtagct 124020 catcctgaat gatctaattc caaaatcttgcatatatttg actgcttcct atttagaagt 124080 tgtattaaaa gtcaatggat tcttcaaaaagcaaaacatt gagctgccgt atgacccaga 124140 aactccaccc ctaggtatac acccaagataactaaaaaca tgtccacatg aaaattagta 124200 taccaatgtt catagcagca ttattcataatcgccaaaaa gtggaaagaa tccaaacgtc 124260 caagtgacta atgagtaaac aaaatgtggtatatccatac aatgaaatat tatttggcaa 124320 taaaaaggaa tgaagtacta atacatgctacagaactgat gaactttgaa aatattatgt 124380 ttagtgaaag aaactaaaca ccaaagaccacatattgtat aattccatgt atatgaaatg 124440 tccaggctat gaaaatccat agaggcagaaaaaaatcagt ggttatgcca tgaaatgggg 124500 agtgactgct aatggatata aggtttcttttgagggtaaa aaaagtattc cagtatttga 124560 tagtggtgaa ggatgtaccc tctgtgagtacagtaaaaaa cactgggctg gacgccgtgg 124620 ctcacacctg taatcccagc actttgggaggctgaggctg gcggatcaag aggtcaggag 124680 atcgagacca tcctggctaa catggtgaaaccccgtctct actaaaaata cgaaaaatta 124740 gccaggcgtg gtggcgggcg cctgtagtcccagctactca ggaggctgag gcaggagaat 124800 ggcgtgaacc caggaggcgg agcttgcagtgagccgagat cgtgccactg cactccagcc 124860 tgagtgacag agcaagactc ctcctcaaaaaaaaaaaaaa cactgaattg cacacttttt 124920 aaaaagtgaa cttcactgta tttgaattatatctcaataa agctgttatt ttaaaaaaaa 124980 aaaaaaaaag caatggcttt tcaaatccttcctgaacttc cttggcctct ttttgcctgt 125040 ttttatttgt gtctggttaa tttcctcatatataataaat aaaaataatt acaggaataa 125100 atatgagaga tttgtcagtg tgtatatcactataacatgt tcattctgga atttttattg 125160 caattttgct tctaaaattg gaacgtaataatgtagaaaa ttcagtgtca ttgggtactg 125220 ttatgacagc aagaacttct gaaatgttttctaaatatgc agtatctcta agtccaagga 125280 tcatttcaaa atatgaaata aacacaagacttagtgtgac ttctttttta cttctatccc 125340 ttctcccatc ctcacaaaac cagtacccatccatgggaaa acagaaaaat ggccaacctc 125400 caactaactc caggggcaag aatgaccagcatgccctagg attgagagag gaaagagaga 125460 aggaggggcc tgtatttctg ccttgacaacgtagtaagta aaacgtagca cactgttgcc 125520 cgtagtttca accagtggat tagcagccccaccttctagg tgaggctaga gagggtgaag 125580 aaaggggcaa catgtccttg agttctgtccccatgcctag agagaggaag aacaatctcc 125640 tcccagacag aagcactcag taaatgcaggcagtttttat atattctcta gcccacacga 125700 cattcaaata aggctcaaga aaggttagctgaattgaatt atcagaaaag ggtaggggga 125760 ggagagatca ctgagtattt atgatgttgtaacagtttta attgaatata agtcaatgga 125820 tcaatttctt gattataaat cttacaattagatttcttca atcagcaagt aacttttggc 125880 agatttcagt aacattaatg cactttctagcttaaatgaa aaatggctga taaaagtcca 125940 aaagataaag tacataagta agaacttacttggttaaaac aaaagattat ttagagtaac 126000 attttattta tttagaaaaa gaaaactggaaattagtatt atctaaattt ttaatatctt 126060 catgctaaag tttagtgaat tttcttatgtgaagtagaca ccttaatctt aaagcaataa 126120 ttggctgggc atggtggctc acacctgtgattttcccagc actttgggag gttgaggcag 126180 gtggattacc tgaggtcagg agttcgagatcagcctggtc aaaatggtga aaccccatct 126240 ctactaaaac tataaaaatt agttggaaatggtggcgcac gcctgtaatc caagctactc 126300 aggaggccga ggcagaagaa tcacttgaacccagggatgg aggtttcagt gagccaagat 126360 cacaccactg cactccagcc tgagcaacagagagagactg cgtctccaat aaaataaata 126420 aataaatcaa taaataaatg caataattagaaaattaacc atttgaacag cttttgacgt 126480 ctctagtgga aaaacacact agattgttggtatgcaaact agggacttca aaatattaag 126540 gagctacata tttccctggc aattttttctcccatttatt ttcttaacaa actctatttc 126600 aatatataga acagggttaa cttctacttacgtcacatcc aaatgatgag atggctttct 126660 gtatacaata gatctggaca gataattgggaagcagagca tctgctacaa attttgactc 126720 atctgagcct agcttagcta atgtaaaagtttaccactta aattaatccc agattatttt 126780 gtacaatctt attacttgga tcattttttatactaggacc taataacatg attacattaa 126840 gaaaaaatat gaattatatg aaaattagaaaataattcca aatggatttg gttcattcac 126900 cttgaattca aagatgacca ggtatttttctggacagtac gagtggaaat tcattttgcc 126960 caaaatgctg aaaatatgac aatctagtcccaaagcacta tgggtttggt tatagattgc 127020 agaactaatt aactttatta ccttattctttccctcaagt catacaccag agtaaaactg 127080 cctctataag agatacagct tcctaaccacacatattaat ttttgggtca tatcaagtag 127140 agttctgccc agctggaagt cactctttttttccactaag actgcatggt tttaacatta 127200 cttgaggata taaaaatatt agttaatcagccattcagga gcctgctctc ctgcattcca 127260 ttaacacctt ctaatacagc ataaacattgtaaaggttat attcttggac aggccagaat 127320 gtctttgaaa aattattaga tcacccccagaagagaacta taacacaaat gaatgagtca 127380 caggactaac tagaagcagg gcagtatctagttttcagaa atacagttaa ctaccacatt 127440 aaaagacttc ttttctaatg ttcaactgctttcacctctc tttctggcta tagttggagt 127500 gggggaaagg gctgtgagga ggggaggaagatcattattc aaaggcaccc aagcaaaaaa 127560 ctaggcaagt tgtattattt taaaatattaattaataaag ttatactggt tagcaggagt 127620 ctagatgtgc catacctaaa ataaatgtaatcaagccccc caaaattata aagttttaaa 127680 gtttagctac aagtataaag tttagagaagttgaaaaccc agcacccaga agagttcttt 127740 agaagagttc ttttatagtt ccattatctacccaaaaaag gaggcggaaa gatttagcag 127800 gtaagatgtt ttgaagtaac agatgttctggaaattaagg atatgtagtc acaggccctg 127860 caactctcaa ttaatttgca caatgttgctcaatgaaaaa taatgaggtg gatacattaa 127920 tcagcagtga ggtcttctct agccttccacactcaacagt actgagaaat caaatcgact 127980 tccttctgcc tcagctggat agggatgattatcttactat ccaacactag actgtaaggt 128040 taagaaaaac ccactaagtc actgaaaaaaaaaaaaagac tataattaat gctttgtatt 128100 aactttaaaa gaaagtccat taatttggtaacagaatgca agtgtgaatc ctattaaata 128160 gcactcagga aaggcaaaca attcacttctaattgcatat ataaacgaat ataatgtcat 128220 aaaataagag accataaatc aaaatacagctaaagcatta ctgtcctgta tattctatgc 128280 attttctcac tggcaaaaaa actagcaaaaacccaaaatg acatattcaa agcataatct 128340 tagacagcaa ccttgctaaa ctcaaacaagcttccttgtt taaaattttc ttctgaaaaa 128400 attaattctg cccaaaataa caagattcataatgtgcaaa ataaaaaatc ccttactggt 128460 ctattgttgt ttattattgt tatctattattgttgttttc aaatcctcta aataccatat 128520 aatagtgaca aaatttaatt cgactttttcctacttgaaa acgagtagcg cacattatta 128580 aacccactga cacttgtgta tatgttttcaacaaaatcta cagattcgcc aacgttgaat 128640 ttctaagtac accacaaata attgatatcacaagggaaga aatgtactcc ccagagaaca 128700 taagatataa atataaatag tatataaatcttaattgtat ctaaacagat acaaataacc 128760 tctatatctg tctatgtgta ttcaaatctacggtaaatat atccaactca atacccagtc 128820 cgatttaatt ccaacatgga gtaaagggaaggtaatgaaa attcccttag acctctatgg 128880 tagctgcagt tatattcaaa tcaaaacaataggaactgta cttggcacaa tcataataca 128940 gccttataat ataaggttag gaatgaaaatggactaaacc attttcattg aaggtaactt 129000 gggcagaagt aacaatgtgc aacgtattgagaaaaataag gtgtgaaaca aaaggggttg 129060 cagaattaag ttccaggata aaatactgtccaaggggaga taaaaaagga aatagacaaa 129120 agtctcaaag ggacaactag gtttttaaaatgtttcttac aggcattatt cagtgatcct 129180 atgctctatt taaactctac ttcatcggcaagaaaagaat ttcatgcaga tagatattgt 129240 gcaatggaat gtaacaaagc attgctccaaggtattgttt attaatttct gtcactgatt 129300 ttggatccat tcctaatggt aactaaaagctttttgaatt gaaaacatta aagggctgtt 129360 aaaagaaaca aatgcagaga gcagtacatcccgcagcata accagccagg gtgggtgggg 129420 gaagggtctt cacatttaga cagcactggcaagaaagcag gtttttttaa tactactaaa 129480 attatgttag ttttcaacaa ctaaaaatattctgcaatag ctgtttcacc taaggtaatt 129540 tattctactt agaaaagcaa ctaatgaattgataagtcaa ttgaaaagtg agtgagtcat 129600 atttttaatg tcaaattgca aaattctttatgcttgaaaa gaattagaat taagaaatct 129660 gctaaaattt tggctaaatg agcacattttaaggggtttt taaaagaaaa atttaagttc 129720 cctcagacct atataaaatt ggtctagaatccatccattc atcatattta tttacagcca 129780 agccaaagct cattccttta ggactacagcaaaatccagc aatcaacctg tagccaagga 129840 gctgaatatt catagagaaa aatcacataagcaagcgaat gtattttatt ttagattcat 129900 tatcgccacc ccaatacctg ctcttctctccttcaaccac cccaacacac acacctgacc 129960 ccaatctcag atgatgacct tacatttcactgggaaaaca aaacccttta ggtaagagct 130020 cccccaatcc ccaccatcaa gttacaaaccttggagcatc catttccagt gtccctttgc 130080 cccctcctat taccagggag agatgtccctctctccaaac aatactaagc tcccctacat 130140 gtgctgtcga ccttcctcaa agcacaaaagacccatccta gggaagaaag tgcagggatg 130200 cctggaagtg atctccctcc tctctaactgtaaaaatact acctaatttt ttaatgtagc 130260 tatttcctcc cactagaata taagctccatgagagcacag acattatctt gttcacctgg 130320 ctgtctaaca catgcaaggg tgtccatttttggagctgtg aaataaaaat tccttaaata 130380 tttgttgcaa gtcctatctt ctattaagccttccccttct cccaccactc ccatcgaaag 130440 cttcattcat tagcatgacc ccagctgcatccagggaaaa ttaattacta catcttttgg 130500 gctcccataa catttgaatc cctcctacaacacagaagag acatttttat atacattctt 130560 cctaactccg attctcattc cacactataaaacaaggtac ttaagaggaa cgactgcctt 130620 atttatcggt tattagatcc taaatcaatgcctgaatgag ctcaagagag tttctttaac 130680 tgtactgagg agaaagtcca aactactttatgaattgatt tctaactcat cccctacttt 130740 ttcaaaagaa atatgtaaga taaaatacctatttaataaa taacaaaata ttacttttct 130800 ttacttgcag aagttattta gtcatgaagtagacaagcag ggtaccactt aacactaatt 130860 taatggaaag atagtctaag tttgtaatgagaaaacggaa aatggttttt ccccaaggtc 130920 acagactaag gcagtggcaa agtcaattaactactcttta tcttaccaac accaaaaaga 130980 gctttcattt cccaccaacc cacatccatagatttcaaga tttttattat tatggaagta 131040 gttgataatt ttgtctatta gtaaaagaagagtaaagttt taattaatta tactgaataa 131100 aagtgcaggg gtgggtggag ctattaaaagaacaaacagg ttttcttctg ggctatacca 131160 cagagagctg aacaaaagcc acttgaagaaaactccctca gagtacaaga gctgcttttc 131220 tctgtagatt cttacaagat aatgatcaagcaagaaaaac acctaatttc ctttttttct 131280 tggattctta tatttcccag agagattatattcacaatgt ggaaagtgaa actgttagaa 131340 gaatctcact tacttattaa ccccagtaaacttaaaaatt aaataaaata cggcatcagc 131400 aatttggagc ctctggttaa taataaagggtataaacagt gaagcaagaa actctgcgtt 131460 aatcaactat gtcgatctgt cattctgtgtttccttaaag tgaaaatggt gaactatgct 131520 gaaaaacaaa ataagataaa cctttgtgtgtgactaaaaa tctagccagc cgagtttctg 131580 gctagcagat atggcagaat ggcatattcaagacttggaa agactaacat tatctataag 131640 tccgcaattt acaagaagta gttttaacagtgacatgaaa aagataagaa aaggttccaa 131700 ataaagttat tcctattatg atttcaatattgttatttgc atgaatgatt atgaactatt 131760 ttttcatcca ttcgttcagg cacttatttataggaagtac tagaatgtca cagaggtaat 131820 aactaaactc ccaattgttt aaattttttcatagtgaaaa taaatattca tatggaaagc 131880 ttcataaagt aaatagtttc aaaataaaacagaatgacaa aataaccagt taaatcacac 131940 tatcaaagtg aatgaaccca aattttttaatttttcaaac tatacagagt ccctcatttt 132000 cagctgctcc acatctaagt gttctcgtgttttcttagcg gcagcactga acttcagagt 132060 caatcaagtc caatttttag gatgcctttggaattgaagt cttaagacca caactaacaa 132120 aaagcaagta ctttgctcct tcacagcaggatgcagattc ctttttctaa gcagaattcc 132180 agacattcca tgagtttcct ataaaaatagatgtccattt taaagtaggc cagcctgaac 132240 tcataccagc ataaaatagc caagaagcacacttatgact gtgaggttta catctgctct 132300 tgtaagcaaa ctctagagat taccattcttcagctccacc ctaaatctgc ttctcagctc 132360 ttccttacta tcacaagaca gtgggcgctgtgaaaagatg gcctagtggt tcaaggaaaa 132420 accattgatc aggagagctc tgaggacaaacatggacgag tgcttgactt tcccttcccc 132480 ctccaaataa actgctgcat tagaaaactgatattacatt taaaggttat cacttctcat 132540 aactcaatga cttgattaaa gaaaataatttttttaataa atcaaaagta ccatgagttt 132600 tttttaacat gacacaacag catacactataatatcatga agagtacaaa gcttaaaaaa 132660 agaatttaag tgtagacatc agagtaaaggcctggttctt agtctctggt agcacatgta 132720 gagcttgact taagtgggat ttacaccggtctctgtctat ccttagatgc ccccaggagt 132780 gagatggaaa gcataggctt atcagaagaggattgtgtga cccttttaag ccctttattc 132840 aaaaagactt atcagatccc tactctgtgtcaggaccgtg cttggcaact gtaagccgaa 132900 acagacccag tcactgccct tctggagctgaagcttgctg agggaaacag acaatcacac 132960 aaaggaacac attattacct cccatgagcagttctaggag gcagtcttag cgggagagtg 133020 agagagagag acccaatcta ttccagggatcagggaaagc tttcccaaag ggagaaatgt 133080 ttacaccaag tggaagttaa ccaggcataaaaggaaaggg aaaggacatt ccaaaattag 133140 aaatcaattt aggcaaaggc ctgaagcaagaggggcaaag cctgtcagag gaattagtga 133200 gtttgaaaag gcaggtgttg agggttggtgtctagtaatg taactagtga aagttgaggc 133260 tggtgggtgc taggccacag ttcatgtctagtctttcagg ctttattaaa gtcctgagaa 133320 gcagagaata acatgaccag aatgtctgtagagtgaagaa cggggttgaa aataaaccta 133380 cagcagacag gaggaaaaca gtggctggcctattgcagta aacttaggat aataatggta 133440 gcctaaacca agagggtggc aatggaaaagagagaagtgg gccgttttta atgatctcag 133500 atacagtact tttgatgatc ttctaatgaactatatcatt aaatttccaa aaaaaaaaaa 133560 aaaacctggc ccgtaataag cattccatgaatatttgttg attcagttaa tcttcttcat 133620 tccatgcttc ttccttcttc ctcgccttttttgtgaagtc ccctccccag gtctgtctgg 133680 agtggaccca ccttgcagaa gcaatttctccagaacaaaa atctgtcaac tcatatctta 133740 atatgtcgac actaaaggaa taagaggatacaaaggaaaa atactaggga gaaaaattct 133800 ctctgtcttc aagttgaaat tttcatgtctctaaccagca ttttgcagct tgacttcttt 133860 tgggtattta agaaaattag cctatattgtgtttcctgct atgaacacca ggtgccatac 133920 tatgtttcat ctacactatt taaatgacatgccaacttca ttccttttat tgccagatca 133980 gctccctaga ggtcagagtg accgtgtcacttgtctggtc acagagccag agagaacatg 134040 aactgggaga tcaggtcagt tggaatctaaacccagcctt tccttgcttg cttaccacac 134100 tagttctcaa gtagctacaa ttccttcgcctcgctttttc ttcacatata ttgactggcc 134160 acccatttaa aaataataaa tacgtccactgcagcatgat gttagtttgc tgaaagctag 134220 gaataagaca caggttattc tttctagtgtggcccagaat taaccataag atcttaagca 134280 aagtattctg cttctatttt ttcgttggtcagatgaagat aatgatataa gtatatgaat 134340 tatgagtatt attgttagta atgagagttctaagcagtag acacagagag gtgccactcc 134400 atgcccttca agaaaggcct ttgtgcccagctgtggagtg ggtggtcagc agacagcctt 134460 caggaggcag caaccttagg gccagcctcagctgcagagt gctgcttgcc tgagctctcc 134520 cactttccaa ggtggcccat attcagtccctgaacaaggg tgacagaagg gctgtccact 134580 tcagcctaca caggccgcca ctgactagaaacccttgctg cattcacaag gcttgccagg 134640 cctgcatggc agttcagctc ctctctctacccaaaactgc ttcctccccc ttccttttac 134700 aagtaatgat tcctaataaa catcctgtaccccaaattct atcttggcag tgcttctaaa 134760 gaacccaaac catgacactg tgctttcttactttggagat attaactagg tcatcataaa 134820 acttaattac atatttgaag tgccaagaacaggcctgttg agtaacttga tttgaagttg 134880 ttctattaat taacagaaag atatctttatttcatatttt aaacaaatca agtcctagtt 134940 gatagccttt tttaatctca atttaattatatgccaagtt tgaacattca agtttttatc 135000 ccagataact ttggaagaaa gttatgaggctaaaaattaa aggataaaac taaattggga 135060 catctaatat aattagagtg tcatttcagtgacaacaaat acatttcagt ctagttttta 135120 atgtgttaca caaagctggt tctataagtgcttggcatca ataaattaac aaccagcatt 135180 tttgccagca aagccagcag catgcatataaaaccaacaa gggatattag gcccactgcc 135240 aaagctttat acaactagtg tcacttaatttagttctcat atcagagtgg aatcatgctc 135300 gttctggaaa agtatttctt ccaaccctcccggaaactgg aaattaatat atttctctgc 135360 ttgcttatag ccaacactac aattttacttttatctaaat ctggaggtaa tgtttaaaga 135420 ctgtaatttt aagctatcca agtgaggataagttttaaaa cattacatgg atagtggtga 135480 taacaagtga aatggtcatt ctaaacccctaaataatatg accactgaat cagatatgca 135540 tagagcccaa aaggtccttt cattcaacgtccagaatgta ttcattcaaa tattatttag 135600 tgagcaccaa ctatgtgcca gtcaatgtctgggtaggcag agctattccg atagtggtta 135660 tcttgcttgg tgaacagaaa caggaatgcaggaaacaaat tctgccaaaa gatccctaca 135720 cttaagaaat ggcaaaaaat atactcctacatattaaaaa taaatgaaaa gaaccatata 135780 ttagatgcaa acaaaagatt catgattattttctctcttt tgtctttttc tttttctttt 135840 tttagagaca gagtctcgct ctgtagcccaggctggagtg cagtggtgcg atcatagctc 135900 actgcagcct caatattatt ttcttaatggtgtccaaaag aatactgaag aaaggtcagc 135960 ctggaatgtt gaaccctact tatttcataggcagaaacag ctgccaggtt ttggtcaact 136020 gctgtcttac taagactgcc aaatggaaagcccaataggg ctgccatgtt atactgcatg 136080 tcattcaaat gttaccaatc acttaacattcattgtaaat actgagtgtt taggaatgcc 136140 acagaaattc tggagagtca caaacttctaaaggtgtcgt aagcacttac gcaacatttt 136200 tataagcaaa atctgagaag tatgatactatttttcattt tagaattcag tcatattctt 136260 cactaaattc aaaaccaaac cacatatataccactcaggc cagagggagg ctgaaaaaat 136320 aacacttgac aaactgacag aaaaatgctacctcctgcag agaaatagag gtgttaaaac 136380 aaatcaggtt gattcttgtt tttattaatgtgtaatcatt tacaggacat acttcatttg 136440 caaagaaaat acttgttcat caagttgaaagatgaatgat aatcgctttt tgagcatttt 136500 atattaactg gattaaggta gggaactagcagaagggcaa aaggcaaaat ttaacaattc 136560 ttcctaaaat ttatttgtca attttttggtgttccagaga agaaaataat tttttttcac 136620 gttttccatc actcccaaac aattttaaacagaaaatttt attattttat cctttagttc 136680 attatgatga attaaatact tgataaaaggagaaaagtaa actatctttt tatgttgatt 136740 ttttttaaaa agaaaaccaa ctgtgtggaatgagggaatc agagactaat tatatgaaga 136800 ctatgttaat gtttaatttg gttatattttaaaaaacctg aaggctcctg cagactgttt 136860 tcattctatg ctactacccc actcctgggcgcaaccacca aattttagtg ccaccctcat 136920 aaatgccaag ggagtaagtt tatttttcccaaggttcaac agagatctct tgtgcttagt 136980 ctgagggcag tggagtcctg cattaatgcatatttttatt aagctctcac tttagtttag 137040 catgcaacag atattttcca aaggtttttataaaccttcc tagttttctg acttaaaaac 137100 ccaaaataca gtcatgaagc ccacgtatctaaatattcct gctccaaagt gaataatgca 137160 aaaatctctc aaagttacca ttctcatcattaaatcattt tagatttaat tttcagtgca 137220 aaataaccaa atagccccca aagtttccagaacctgttgc ctcagcaggg agctagcctt 137280 cccaaagagc acagagaatc caactagagtcacctaccca ccaggtctca ccagatacag 137340 gttccacaaa ctttcctgca tccagaaaatataggaagag ataaagacct gggggaatag 137400 catttgcata agcagcggtt atgactgtgggttttggagt caaggagact agcgactacc 137460 tcctgatttt gccaattact aattgtatgacactgggcta gttgctgaat ctgtccaagc 137520 tcagcttccc tgtgtcaaga tgaaggcgtcaagatgaaga tctgctttag ggaattcggt 137580 gagaatccag caagatgctg agggtagggcattcagcaca gcggctgcct tagcagtgct 137640 ccatgaagag gcgattgctg tgattactgtcgtcctcaac attgtgatca gcccaaacac 137700 ctacatctgt cagctatgct tagaaagggcttggctgtga gcaacactat tgactgtctc 137760 tcccttctct ccaggaatag tacagacaagatagaagagt cagtggttga gaactaagag 137820 gtaagaatat tttggcactg cggttctttataacacttag gaattaaagc aattggcacc 137880 ttgagtacac tcaagggaaa atcagatcagtggcttgaaa gtgcatccag tatacttgga 137940 aatgcaaaaa ttaattatat ttgatggcgatataataata atagtaataa agatacactt 138000 taacacaaca acctcagagg taatatcacacagatttttc agataactca cggtagttat 138060 taaaatttct gaagaccaca tagctggtaaggagaagagc caaaattaga atctaagtct 138120 gtttcattcc aaatgttcat aatcactgtaatctgttcca agtgtcctcc caacaagcta 138180 gaagcctcat acaaaaccac tacatgatggaatcagatgg atgagatggg gtaggatgga 138240 aactcatggc atgctcaata cagaactatgtggcctaaaa aaccagaaga aatggtcagg 138300 ccttctacag aacattccgc acaaagtgtcaatctaaaat gagggccatt aatcatctgg 138360 taattttaag atcaaacaac tgagaccacaggataatcac ggaacttgtc agtcctagaa 138420 cgtatggact ctaacgcata tgtcagtcatgacatcaagt ctcacaacca aaaactgccc 138480 aaaaggtttc tgaaaaagag aaactttaagaataacaatg aagaacaaat acggacattt 138540 tttaaaattc tctttttact aacctaggagtaaacgcaac acagtctcat aaaagcaaat 138600 atcaaatttc cttttttaac tacaaagttagattaatcat aatccttagt tcgtaacaca 138660 tatgcttttt gtttaagagc cagggtctcacttggtcatc caggctagaa tacagtgacc 138720 caatcatagc tcactgcagc ctcaaactcctgggctcaaa gaatcctcct gcctcagcct 138780 cccaagtagc tgggaccaca ggcacacgccaccacacctg gttatttttt tttatttctg 138840 gtagagacga ggtctcgctt tgtttgcctagccttgtctc agactcccgt cctcaagcaa 138900 tcctcctgcc acagcctccc aaagcactgggattacgaag atgagccatt gagcctggct 138960 cgtaacacat acatttatct ctccttaatggtccatgcat ggcctgcctt tatatttaat 139020 catttatatt ttatgcatgt atttgtatgttttagataat acgatacacc ctcatgaacc 139080 caccacctga cacaagaact agaacattaaaaatttacac ctatcaagtg tttctcttca 139140 acccacctgc attaactgct atcatgaataataataattg gcttattttt ctatatatat 139200 atcatatatt ttgtatatat cttaaaagcatttttttagt tttagtttta attttaacac 139260 attaaaagtt taaaggagaa aaaggatgtcatataaatag attcataaaa gcatttaata 139320 aaattttata taaatccctg atataaactttagtaaatta agtatagaga agggaacatc 139380 cttaacccga cccgatttaa tttttttttttttttctgag acagagtctc gctctgtcgc 139440 ccaggctgga gtgcagtggc gcgatctcggctcactgcaa gctccgcctc ccgggttcat 139500 gccattctcc tgcctcagcc taccaagtagctgggactac aggcacccac caccacaccc 139560 agctaatttt ttgtagtttt agtagagacagggtttcacc gtgttagcca ggatggtctt 139620 gatctcctga ccttgtgatc cgcccgcctcggcctcccaa agtgctagga ttacaggcgt 139680 gagccactgc atccggccaa aattaagtacaattctagtc aaaatcgtac atgggtcgtt 139740 catggaactt gagaaggtga ttttaaaattcacatggtaa aacagccaag atacttttga 139800 aaacgaaaag caaggagggg acctgcaccctatcagatat cagcatttct tacaaggcta 139860 agacaatcta actctggaca gggagagacatattgaccta tggagtagaa gagggcccag 139920 aaaaaaaaaa atcccacaca tatgcataactttaatatat gacagagatg gtgagtttat 139980 gtgaataaaa atgaaaactg aacctccattttacattaca caaaaattaa ctccagatgg 140040 attaaggact taaaatatca gaagcatatctttaaaatgt ttagtggaaa atatgtttta 140100 aggacttctt tctgaatctg ataaaaaagtattttttaaa gacacaaaag tacccattat 140160 aaaacaagat acagtattag taaatttgactcttttaaaa acaaggacat acgaccatca 140220 aaagtcacct tataaaaagt ggaaaaaagctacaaactgg gagaggatag ctcccatctg 140280 tataaccacc aaaagattag aatcaagaatatataaagaa cttctataat aacaagaaaa 140340 actcagacaa ttcaaaaaaa actgggcaaaagacatgaat aggctttttt tttaaagggg 140400 gggtggaata tattagacat aagtatttgaataaatattt gacctcatta gtgatcaggg 140460 aaatgcacat caattataaa ctcacataattggcaaaaat ttttaagtcg gatagtagcc 140520 agtgtttcaa gagaatatgg atgaaccagatttcttaaat attgctggta ggaatgtaaa 140580 ctggtacaac tactttggaa ggcattattgcattatctta taacattcag atactctata 140640 tctgtatata tacattcctc tgtgccattcctctgtgaaa tgccattcct ctgtgaaaac 140700 tagaggaaat tttgtacatg tagagcagataacatgtaca agaatgttta tcacagtagc 140760 attcataacg aaaatctgaa aataacccaaatgtctattt gagaaaatgc agaaataaat 140820 ttaggtacat tctcactatg gaatgttatacagtcctaaa aaagggtgca ccactgcata 140880 caaaaatata gatagatctt ggcagcataatacagactga aaaattaact cctgaaagat 140940 gaccaatacc aagatactct tttatcaaatataaacttaa atataaaatg taaggatata 141000 ttaaaccagg gatcaacgta acaatcaaactgaaatccct acctcaataa aaactgatct 141060 taaaagtaca aacctccaga atttccatggtgtcatatgc agtctccttc acttatttca 141120 tgtgataaag ttctgcattt catcaagatgaagtgagatg caaatagata aaaccacaac 141180 tgtacagcag tagatattac accctctctcattatttatt aggaatagtt tgcctgctca 141240 cttaagaatg aatatcctag cgctttggaaggctgaggca ggtggattgc ctgagctcag 141300 gagttcgaga ccagcctggg ctacacggtgaaaccctgtc tctactaaaa atacaaaaaa 141360 aaaaaaaaat agccgtgcgt ggcagtgtgtgcctgtagtt ccagctactt gaaaggctga 141420 ggcagaagaa tggcgtgaac ccggaaggcggaggttgcag tgagccaaaa tcgcgccact 141480 gcacttcagc ctgggtgaca gagtgagactccatctcaaa aaaaaaaaaa aaaatgaata 141540 tcatacctca tacactcagt cataaagagtcttgagaaac tagtggacca gaaaaatact 141600 aggtgtatta tcctgaaaaa gcacactctggaaaacagct cagtcacatc attcatcttg 141660 ccacaataca gtgtccatct gtgaccatttttgagtgaat aaggtcatac atttgcttaa 141720 agataatttt tgggcccagt aaggaaatctctttagctcc taaattagaa gccagaggag 141780 cctggcagcc ttcacatgtg agaaacctattgagcagtga atagaataat tattcctgaa 141840 gactgtgtgc tctggatata ttcctgaagactgtgtgttc acggagcagc tcagtgctcc 141900 gtgaaaggca catttgcctt cccaactctcagaatatact aaaaatgagt tttttcccct 141960 cctaaatgtc attagcatat tctgataataaacaactgtc tttaatagcc agatatttag 142020 tatagtagtt atacatatat agagatgtagccactcagcc tattgtcctg aaaactggaa 142080 aatctgctct tcctgtccta aatattaaggaagtacatag cagatgctgg agaatctgta 142140 aaattaaatg taacaggatt accatgagttttcacttact ataggtattt agattccatt 142200 cttacatgac ttatccacac tgataaagaatacgcataaa attagtgtct tttctgcctt 142260 tgataaagaa caaaatagtg gtaagatagttttaaacaat atgagttgag ttcttaatta 142320 actgttgcaa gtgtcagagt agtgttctatttgtgagtgg cagaaagtat caagacacta 142380 ggagagggca gttaaggaag cccccataaaactatggaaa tgagaatatg gtacaaaggt 142440 agacaaattc cttgttacag aaataaggatatcgaaatga attaggtttt ttggatcaat 142500 aatgtcaaaa accattacgt atgtgattcattaaatgcaa taaccaaaat aaaaatattt 142560 ttcttttcag aaaagtattc catgatgaaatcagtagatt tcaaaagaac aaacatacac 142620 attccaaaac cacttgcatg tattgacaatgttggtgaca tatgaattca tatgagcttt 142680 ctggagagaa caggcttgag cttccccatatatgtttctg tcctcccaac tgggctgtga 142740 gttaagaaca gggactgtct tattccttgttgtatcccaa gggtgtgatt caattctggt 142800 gcttagaaaa tattttttga ctgaatgaatgaacttggta ttatatacca agcactttta 142860 aaattatttt cacacccctt taaaccagtatttctacccc taggaattta tctgaaggaa 142920 ataaccagag acacaaagac acatatacaggatgtttgtt agagtattat atctaatagc 142980 acaaaatcta aatgttcaat cacagagaaatgataaaatg tcataaatca cacaaggaac 143040 gtagtactag gcagttattt aaaagtgtgtttacgaccgg gcgtggtggc ccagcacttt 143100 gggaggccga ggcaggcaga tcacgaggtcaagagatgga gaccatcctg gccaacatgg 143160 tgaaaccccg tcgctactaa aaatacaaaaattggctggg tgtggtggtg tatgcctgta 143220 gtcccagtta ctcgggaggc tgaggcaggagaatcacttg aaccagggag ttggaggttg 143280 ccgtgagcca agatcgcacc actgcactacagcctagtga cagagcgaga attcgtctca 143340 aaaaaaaaaa aaagtctgtt tacgaagtataattattgac aaggaaaatt ttcataatgt 143400 aacataatat gtccagtata tttaatacatatatacacct agattctaat gtattatata 143460 catatgcatg agtatattta tatctgtaggaaaaaaagtg tgaaaataca ccaaattgtt 143520 aacatatttt gctaataaat agaacttagctgatatactt taaaatttac aaaagtaact 143580 aaaattttat taaaaatgaa atataaataattttaattaa taaacatact ccatttgcgt 143640 atgtgctcta tctagaaact atccgacaaccacaaaaaag catttgatat ttagtcttat 143700 aattcagaaa tgagaccttc ataaggaaaaggaaaataca caaatatggt ttaaccacca 143760 caagagcaac tccaccaaaa tgagcacatactttaaaaat agtcatgcaa gtaaaatatc 143820 tcattggaaa ggtaaatatt agtgaatattttattggaaa tataaaaaca ttcgtattta 143880 gagtaatgta atcgggaaac ataccttacaaaaatattct aaaatatttt aaaataatat 143940 acactagaaa acaaatacag ttgtttccaaattttacttt ctaaaaataa aatcctacta 144000 ctaattctta acaagggtat ggtattttccttttttaatg ctgttttacg ttatcactcc 144060 ctaaagtcaa taactgtatc agaattctgggctcatctga tgaaaacatt ccatccctat 144120 tggaaaacaa tctgaaatca catagcctcagtctgcttcc ctgtatagtc catacaaacc 144180 aaaccacaaa agctactaaa attaaacagtggagaattga ttcactgata ggccagcaaa 144240 aggtttttag gttttttaat tctttgggtattgttttgtt attgttgtta tcttttttct 144300 ttaatccagg gaaagtccct actacaaaggtctcaaaaca gaattgaaaa gaatggatct 144360 tgtctgggtg ttcagctatc catatacacaggttcattct tcagcagagt ttactaagct 144420 gtttagtgtc tttgaatcag tttttctctttgttaattaa aacaagctaa atttgccttt 144480 tatgaattct taggaagttc tattctcagataacctatat taaactttta agaaagatgg 144540 agaatggttc agaataatta ttacagtagccaagaagtcc aaaaacgcta acggctaagg 144600 aataaggaac cctgaatatc ttaaaatctagctttacaag ccaaagacat caagacaaat 144660 attgtaaggc cttgtgcatt tgtctgacctgttgcgccga caaactgaca ggggaagaga 144720 atacccaaat tctggtcata ggctcagctactattcagcc gagacaacct tggaatctaa 144780 cttaaaaata agagttctgg gccaggtatggtggctcgcg cctgtcatcc cagcattttg 144840 ggaggctgag gtgggaagat catttgagccaaggagcttg aggttacagt gagctatgat 144900 caggccactg cactctggcc tgggcagcagaatgaaaccc tgtctctgaa aacaaaataa 144960 aacaaaaaac aaaaatcctc atgtaaagataacctgatca aacaggagaa tttactacta 145020 tatgcaagaa tattgcagtt actgacaggaatatttatgc taaagcattt taatgattat 145080 tcaatgcaaa aaaagtatta attgattaaggggggaaaac aacattatca caaacaacgt 145140 catcacaggc atctccctag ctacataattttccacttgt cacaaatgca aattttaaca 145200 ttactatttg aaatagccac aaggtaccaaatatattatt tatagattat aaaccttcag 145260 ctttttcagg cctgaaactt agcattcatttgaacgttta taaccacgta actgaagtct 145320 atacaacatt aatctgaagt agtgaactcttgagcaagct cccacagtac ttaactaatt 145380 tcagtgaaga aagtgcacct cttaaaacatgccagtgata gaactgggga aatgcaaaga 145440 ataggcctga aattctagac catggggagatcttaaaaca tagcaagact ctgtctcaaa 145500 aaaaataata ataatgtaag aaaaaaaattctattaataa acatctagca atcactatct 145560 caaaaaccaa caaaagatga gaatataaacaagtttacaa aagtatctga taaatgcata 145620 gataatagag caggaaccag ttcttagagtgtatttattc agagcatctg acagacaatg 145680 ctatagatgg ggtggcaggg actgtgagttgcctcccaat gtctgttttc ttttgacatg 145740 gtatcataat ttctagttgc ccatacagtcacttaactca agactcattt cccagccttc 145800 cttgctgctg tgtatggtca catgactaagatttggccaa tgggatgtga gtatacatat 145860 tacgcacaac ttctcagtca tgatctttgccctagttaga atctgattga aatggtcaga 145920 gccatcttag gccatgaaat agtagatacatgagcatcaa ggtacaagga gcctgggtcc 145980 ttggtaactg tagaaccaca gtcaggcctggaccttcatt ctagccatgt agatagtagg 146040 gaaataaata tctatcttgt ttgtaccatgattacttgag ttttctctat aaataacaat 146100 tattagtcaa aataatacaa tagctaacatttatttggca tttactacat accagacact 146160 gtgctaagag ctttatataa actctctcatttgctcaaga cagtaacagt ttcaagcaat 146220 tattcctatc ctcagaaagc caggccttatggagaaaaga taactttctc agagtcattc 146280 agctactagg gcctgaacat aggcctatctgatgctgatg ccatggtcag gctgtgagcc 146340 acagccacat acacaataaa ttccatttgcctgggatgga gaccagccac gacccccttc 146400 tacaaagcat tccaagaacg ttgagttttatgtcccccag atttaactgg catatcttca 146460 ggactgtgaa gaagaaaaaa aaaattgcatcatagcacag aaatgaagaa agagttatac 146520 agccaagatt cacaggggaa aaagaaatcattcccactgc cactccaata caatgcaact 146580 taaagtcatg ccataaagtt atacaccaaaaggtttatag ttttttggct ttggtttttt 146640 gttttttgtt ttttttcagc aggaggagattatagaggac ctttattttc tacattatat 146700 gtttttataa ggtttgaaat ttttataatcatccagtatt acttttatca tctaaagaga 146760 caataaagat tttttaaaac atgtcataatgggtatcaga aattttcctg cttggaattt 146820 tattttttat gagaatgcaa ttgtaagttatgattaaaag tgtcaaagtg ctagagaaat 146880 gtaattatag agataaaata ataaaagaaacataccaatt tttaaaaatc tatttccaac 146940 tatattattc caatatggat ttaaagcagcttataacaac aactacagta taacacagaa 147000 ggagagttaa gtgaaaaaaa ttgaaagcaaaaaggaaagt aaaggtgaaa gaaataagat 147060 gaaaatgatt ccagatgtac agtcagtgtgcaaaacacat gggctcaggt tctgtagcct 147120 tgttacagat accctgaatt ttttttctaagcttcctagc agacaaggca aaaaggagct 147180 cagagtgtcc atcagataat aataagtgaatggttcagaa ggaagcacaa ctgattttgg 147240 caccaggacc tactgagatg ctttctctaatgagtccaca gaatgaaggt gctttttcac 147300 aaaatgaatt gtgtgtccag acacaaacaggatttttttt aaatgttggg gcccagttgc 147360 aagtataaaa gtttaataat ttgggggccaaaatttaact gaaagctgaa aggcatgcat 147420 attatataga aatgcaaata aaatggtctcaatatagttc ctggacaagt ttccaaccac 147480 cttctccttt atacaaatga tatactgtaatttactttcc ataattcact ttctttgcag 147540 atacttatgt cttaaatatt actgaccatttttaaatatg caacactaaa atcattatgg 147600 tctgacattt ttatatgctt ataaaacacattttgttcta actgttttta ttagaagaaa 147660 tgtggcctgc tgcaaaccaa aacctacatattttactttg gaaaaaaaaa agcagctgga 147720 aatataaaac cccacacatt tgcttttaacactgcttggg aactaaaacc tatcatgtgc 147780 acttgactgt gttttcacag aaaagcagcattctcagtgc aggcacagcc cacggcattc 147840 tcactgctcc ccaggaaggg gtgagggggtgactgtgcaa gctggggctc cccggatgag 147900 ggctcccttt cacagggatc tctggaccattgtgtctcta gacttaagaa tacagaaaac 147960 aaaattcctt ggaatttagc ctctgaggaggcaggcaaat tatgttaacc acaagcaaac 148020 atctacatta ctttctggtt tgtttccactaccttccaaa ataaattgca atttaaaatc 148080 agatcttaga agactacata taacaagacacccgtatatt ttcttttctt gcctcatgaa 148140 atgacaaaaa ttgcaaaaac ttttaatagcatttaaattt taatactcta atcacatgtg 148200 tttatattta aataaatgtt ggtttcttaaaatacgatta ttcccaaact ctcttgctat 148260 aaattaaaac acaaatggaa gacgaagatgcccagagatg attccagttg atggaagttc 148320 ctggtccagt acagaaaggg aaagagaataacaataaata taagtcaaaa gaagggtgga 148380 aacaaaatac actggcagta aaggcatgttggtaccaggt gggttcttgg aaaacaggtc 148440 agcagaagtt aacagggtgg tcactagcccagtgagccaa ctgtttccaa aacactggtc 148500 caaacacaag cctacttgtt ctctgctggctggtccctta ttagagattt ccagctggaa 148560 cttcaaaact tgatacaaac catgaccaagtgggctttgt catgggaatg caagactgga 148620 tgagtactca aaaaacaacc gatgtaatccaccagattat cagattaaaa ggaaaaacag 148680 tcatcatcaa tcaatgcaga aaagcatttgacaaaattca acatacattc atgatttaaa 148740 aaaaaaaact ctcagcaaat taggaatagaagggaccttc ttcattctga taacacctac 148800 gtctaacata atacttaatg gtgaaagattgaatgctttc cccctaagac cagaaacaag 148860 gcaaaaatgt ccactcccac cacacctacttgacattgta ctagaagttc tagccaatgc 148920 aagaaaaaaa ctcaaggaaa agaaataaaaggcatgcaga tttgaaataa attaagttgt 148980 tcctattcac agacaacatg actggctatggagaaaatcc caaggaatca aggggaaaat 149040 aatttctaga actaataagt cattttagcaagttcacagg ataaagttca acacaaaaat 149100 taaccatttt tctatatata cacttcttgtatacatagaa accaaaagaa aaaacacaca 149160 tattagaaac caagaaaaaa taccacttacagtgttacaa tttatactta agtacaggtc 149220 tcaaaaagca catacaggat ctacatgctgaagatgacaa aatgctgctg aaagaaatga 149280 aagaagacta aataaataca gaggcataccatgttgatga attggacaat tcggcataat 149340 aaagccatct gttctcacca aacttatctataaatttatg caatcccaat aaaaatccca 149400 gcatgatttt ctgtaggtac agacaagctgattctaaaat ttatatgaaa agacagaaga 149460 actggaataa ccaaaacaat tttgataaaggaaaaataag attggaaaaa tcatataagc 149520 tgattttaaa atttaatata aagcaacagcaataaagaca gtgtggtatt ggcaaaggaa 149580 cagacacatg tgtgtctgaa atttttcatcataaacaatt tttaaaaata aaatttaaaa 149640 taaaccaaat ataaggaatc tggaaaggtcactgaggcac atgcggaaca ttattaaaag 149700 gctggagaac agacactaca gctgtgattcctgcctgtct cattcgctca ctcaaactca 149760 atgcttggca tagagcacat agtccataaatagctgctca atgaataaaa agtgaatgac 149820 gcaccaaggg aaatcttact ctttttccttaaccctatag aaggaaatag caccaagaat 149880 cctaatgaat tgatctcctt aattgaaaaccaaatagaga aagctgcttt aaaaataaat 149940 tttattttag aatgtcaaat cctaaaatagaatgtctttt cctaaatacc ccagcctaca 150000 ctgctttctc ttttatgaac tcctctagtaagtggcctta acatttctta atcaaataca 150060 gtgttaaact gtcctcctgt gtgtggtgcctgggctttac catgtggtga ggatggaaat 150120 agctgacttg tattgatgga gttcctgccatatatcctga cactgttcta agtgctttcc 150180 actcaaccct gcaaagcaag ttattagtatcccctctccc agatgaagaa actggggcat 150240 agagggtcaa gtaacttctc caaggtcacacagctagtcc agggcagagc agggactcaa 150300 acacaggcaa tctggctccg tacttgcctcagtactgcct cttctgagga ccttgagaac 150360 ccagactcct acaaatcaca ctatttttgtgtatttttct cagcaatgtc cacctaggac 150420 aaggttgggt atgcagcaca tactttaaaatctaaatgac tggtgtgcta tctgggctgc 150480 atttgcaaat tccaagagca tctttagggaaagtttttag ttaaagtttg tgttgttctg 150540 aagagccctc ctgaactttc tataatgttttagttaattc caccatgagg atttccatga 150600 caaaaaaaaa ttgttcatgt gtgataaagataaaatgtta gtacttacca gatgttaagt 150660 ttttaatgta aatattgccc aaaatagataccaaaacaag actagcaaag taaaaatcct 150720 cctggggaaa attattttat atctcagtgaaagtcctttc ctataaatcc ccccgccatc 150780 cccaacaaac acacatagaa agcctttgatggcaaaggaa acctcaaaga tgtcttgcac 150840 tgagaaaaca aaacatacaa caccctccaggaattctggt ccttcttaag atctctatca 150900 ctaattgttt tggaaaacct caatacaagtatatgtttca attctgggtt acatgtagtt 150960 taagggtaaa gaaaagaaat gactgtagtcagccatccat tgccatctct ctaatgaggt 151020 atgtgcttac tgaacacatc tcatctgaaaaaaaaatttt tatacaaaag atgagttttt 151080 ctattgatct tttttcttct acagcattctactaaagaga gattaatccc aaaacaacaa 151140 gaaaggcctt taatgaaacc tcacagcagctggccatcac ccaggcttta gctgtgtccc 151200 acgatacact ccagacattc tgcatgctctctgtacatta catgtcagac cattccaatg 151260 gaatgggaaa ctatgattac actataattttaacatttaa cccaagtttt tgctgacata 151320 attagctaaa aatattagca aaaagggtaacaagagctgt ccagttaaca aaactaattt 151380 caaataacaa atttggagga tgataatttcagtgaccaca aagtaaaagt aaaaacttta 151440 tcctatcaag gacagcaata ggctaaccgaattcaccacc tataagggga ttctctagga 151500 aacccaccac gggtacccaa tttccactggcaagaaagag aacacagacg tgattatgtg 151560 accactaggt ggcaccttca cactgcttcatctcaaaacc atatttccac ctccactatg 151620 gccttcccaa agtattagca gtataaaattcaaacccagg tgcctcactt taacaaaaaa 151680 taaatttaaa aaatgagaaa atacattttcaattaaaaac ttttcccatt aatttctaca 151740 tctaaggctt tggtgtataa tcacagaagaatatctcacc aaactgtcaa tataaattgg 151800 tattctgact ttatcgcccc ttggcaatgcattgttccag tattacaatg tagaactgag 151860 ataaatttgt agcttcaact atatgtgtaactgaattaaa ataatttcaa aagccctgtg 151920 agcaaaggct gtgactaact gaaaaaacaaatggtggcct ctaatacttc ttgagtccca 151980 gcctatgacg gcaagtgtca agtaatcctgcagactgggt agaatgtata tcatttcttc 152040 atccctttca aataaatata ttgaagttttaaaagatttt ttaaaacagg tctagattat 152100 gaccttgtag gatctacaaa caattagcaaattcctcaag acttgggtga cattattaga 152160 ttcaaaggga aagtctgagt aatgggcatttcccttattt ggaattatgt tttatgtttt 152220 gtttgttgtt ggcaggagaa tggggcagacagttctgcct acttgtctgt atctatttgc 152280 aaaatatcat cccagtttca ggtagctacaaatggctggg tcccaagtaa accttttaaa 152340 tgtgaggttt ctccttggca atctcaatctctccttttct ctccaatctg tgtccttccc 152400 tgctttgcag cctcttactc aataacactcctgcttttga cacccaatgc cctttcacct 152460 ttctttcttt cttttctttt cctttttttttttttttttt tttttttttt gagacagagt 152520 tttgctcttg ttgcccaggc tggagtgggagtgcaatagc gtgatcttgg ctcagtgcaa 152580 cctctgcctc ctgggttcaa gcaattctcctgcttcagcc tcccaagtag ctaagattac 152640 aggcatgtgc caccacaccc agctaattttgtatttttag tagaaatggg gtttcaccat 152700 gttggccagg ctggtctcaa actcctgccctcaggtgatc cacccgcctt ggcctcccaa 152760 agtcttggga ttacaggcgt gagccaccgcacccagcttt cacctttcta aactgactga 152820 ggcaagcgtc tagaatcagc catttctattctaaggtaaa catgagccag tcacttgcag 152880 agttggtccc tacctcccat ctggttccctggcactttgt atccacctct ttttcaggcc 152940 tgaatacctc acattgtaac tctctgtctacatgagggcc tcctctacca agctaaaagt 153000 ttgtcaagaa agaagaccag gtcttaatctgtatgcccaa aacctaccca tggtgtttgt 153060 aaacattaag cccttaatca atgcttgtttaatgaatgta tcctcttgcc ttgcctgatt 153120 tacattttaa gcctcttctc taactgtggaaagactttac ctctacctaa cacaggagtt 153180 cttaatttta gagttcaaga acacatttttatgtacatct atatgagcat ttttccagga 153240 aagaggttca cagccttaat gagattcttaaaggaatctc taactcccaa aatttggaac 153300 caaaccaagc caaagaactt caagcactaggaatcagaag agaggcatat tattaggcta 153360 aggggcctgc caagcatcca tggaactatattggtaggaa gctaccaact ctgcttatag 153420 gtaatctctc tgaatacaaa gtagaagtagacagactagt cactgctgac aaaatcagac 153480 cagtcgatgc aatcgtatgc attcacaaaatacacaccta aaagaaacca caacttcagg 153540 gagatgacaa tgatcagaat acagatgctcctcaacttac aatgggctta catcctgata 153600 aacccattgt aagttgaaaa tacagtaagccaaaaatcca tttacttaat acacctaacc 153660 tactgaacat catagtttag tccagcctaccttaaacatg ctcagaacac ttatattagc 153720 ctacagttgg gaaaagtcat ctaacacaaagcctatttta taatcaagtg ttgaatatct 153780 catgtaattt cttgaaagct gtacctaaagtgaaaagaag aatgttggta taggtattcg 153840 agttaaggtt tctgctgaat gcatatggcttttgcaccat cataaagttg aaaaatcatt 153900 aaattgacca taataaatca gggaccatctgtactatgat ttctagaaag ccacaaactt 153960 ttaaaactgg gtagcaaagg tataggactgcagagaaaaa tatctatttt tattccagat 154020 tctaagtcct atgcagaagc acagccctgttccacattga gtgtccaaat acatacaggc 154080 ctatgccctc taaaaccctg cttcatgtggctctgaccca gcacccaatc tctttgccta 154140 acacctgtgg gtgaggtggt gtccttctccagttttgtgc ctgctaagta gagcccacca 154200 ctggaaaaat tttcataggc taaattttagtgaattacta agaacaattt aaaattgggg 154260 aagtgtattt tctagaggtc tgtttcaccaagaattttca gtggcattaa ctagtttgga 154320 tgagttattt gacatatgaa gatgtcaggtagatacatcc caggcctctc tgacctggac 154380 tctctgactg actaattata aatcagaggttcccatgatc cactcttcag gttcaacaac 154440 tagaatgact cccagaactc aggaaaatagttaacttact attactgctt tactataaag 154500 gatactactg aggaacagcc aaacggaacagatgcatagg gagatgtacg tggaaggagc 154560 acagaatgtc attgtcctct ctgtgcaccccaccctccca gcacctctac atgtatacca 154620 acccaaaagc tctccaaaac ccatgatttaggggtgttta tgaaagcttc attatatagg 154680 catgattgat taattctttg gtcattggtgatgagcttaa tctccagacc ctctcccctc 154740 cctggacatc tagggtggac tgaaagttccaaccttctaa tcatgctctg gtctttccag 154800 tgaccagcct tcatcctgaa attatctaggagcctccagt caccagtcat ctcattagca 154860 aacaagacac tcttttcact ccagagagtccaagggtctt agaaagtctt gtgtcaggaa 154920 ccgaagacca agaccaacta ttataacaaaagttgctcct atcacccctc tcactcagga 154980 tattacaaag gttttaggaa ttctgtgccaggaactgggg acaaagacta aatatgtatt 155040 tcttattata tcacaatatc acacatacttaatcaaatta ctgtatttat aataatagta 155100 agattagtta tctaaatgtc cacacaaatggcataaatca ctgaagtcct caaaaagaaa 155160 atgttagaag caaatggaga atcatttgagtccattatca ttttctacct ttcttcttct 155220 gatacggctt ttcttgagtc attcatttctcatactgaaa atcactcatt caaaaccagc 155280 cttttaggta gaaggtcagt acccatgtattaggctttaa tgttgggatt cagatgatat 155340 ggagtggagt ttttctacca taaaggggaagatatttgaa taagccttta agaatgagaa 155400 agtgaaaaac agggaagaat aacatgtgcaaagattcagt ggcataaaga aggtttgtat 155460 gttagggaac agcaagtagt tcacagtggtgggcacgctg ggtggactgg gagctatggc 155520 gagagatgag actgaaaagc gtacagccagccagggaatc tggattcatc ttaaatggtt 155580 acagggccat tataggatgt taatcaggggagtgacatat catatttgca ttctggaaag 155640 atcattcttg cagcagtgtg gaaatggactgaggagacta gttgcagaag gagcagttaa 155700 tgtggtaaga cagagataaa ggatttgaaagacattaagg aggaagaata cacttatatg 155760 actcccttca taactgtggg gtaaaaatatagtccaatct cttcttttcc cagttcattg 155820 gttttgagac cccattagtc tatgttattagcaacaattc cagtatctcc ctcctgaagc 155880 tgctccacag ccctacccca aattgttgaaaaatgctgag gtctgtcctg gttccaaata 155940 ccaggtcgaa acagaataga aagcctgatgagtccaagat gagtccacat ttctagctgc 156000 tgcagctcct ggtaaaacca tagagttgttacttttgtcc attgctgcca caaaccctgg 156060 tgttccagat ccctgcaaag acaactgtgatgcctcacac ttgggatccc aggggactgc 156120 tgctttacct taactcatat acactgtcaaaggatcgata ctgtgccaga aacccaggct 156180 cagccacaag ccctccttca tgagcctagaaaggccctaa tgctagccag ttcaacattc 156240 tcctttctcc atagtgagtt tccctttgggccccttggag tcctttcttt ccaagggacc 156300 tctgctggga caacatcaat ccatcagagaggttcgagta aaactgagcc aaaaaagaga 156360 aaggagctag agttggactg cctttcctccaggaagttcc ttggccccta tacaggcaat 156420 cagcactgtg agactttgtc tcgccaccaatttcctgtca ctcttctccc agaatcatgg 156480 ttactcattt gcactatatt tgcataattaatagggttaa gtccagtctt tggggaaggg 156540 tgactttcca tgtccaccta aagagcaagtgcctggacat tcctcacaat ttagaaaaga 156600 aaagaaaaaa agaagtgaaa tgtctgtctttagtgactaa gtagatgggg atgcaactgc 156660 tcctgttctg agtgccagca acagggcagtggccccagat gaaagtcgcc ccactgcaag 156720 gcttggccag tgactggagc ctcctgtgtaagaagaagca gtgtgccagc aagcaccatt 156780 catcaaatgg aacacagcag atttcagccacttctttccc tcagccagtc acctctggac 156840 agtgtacagt gacgcagtcg tcctgcctgaaactaaggca gaatgttctt cttcaagaag 156900 ttaggttccg ggatgagtct gtggcatcagccaattaaag tcctttccaa agagtctcat 156960 ctattaaaga agagagatcc tgataagataaagatggcag aaggggactg tttgatgggt 157020 acactttttt tcatggggct ggggaagtactggactgaaa gtgataaagt ctctcaacta 157080 atgtgactcc ttcctagctc agaaaagagaattatctgtg aattctctca ccctctctga 157140 agtgccagtt accaggggga cagtcatcagtctatcttct ttatacttct ttcaagaggc 157200 ttaaagccct gtgagggggt agatagatagatagatagag agcgagtgat agatagagaa 157260 ttttaaaagc tagactagca attactcaccaaaaagaaaa aacaatttcc tgttttgtgc 157320 tgtcagtaaa atgtaaaatt ttacagaatgcccgcaagtg attctcttta agtgctgcaa 157380 aaggattatt ttctacaaag tcgaaaaaaaaaatttttaa tgctgatagg aaaagatcat 157440 cttattaggt gctttgtaat gtagggggaactccagtcaa taagcagtgt gaggtggaag 157500 gaaagaaccc ccagagggag atgagacttgtacgccacac cagctgcact accaactatg 157560 tggccttcag tgaccacctc acaggaatccttcagttacc cacctggagg gctacttaaa 157620 taccaaacag aatagcttat gtgtgaaatgctttgcaact ataaacgatt attcacctgc 157680 attataatta tgtttctaca aattgtgcttgtcagttcac atcccaaaag gacctaaaca 157740 ttagacacca ttttacaaat tataaaaggtcattatgaag ttctgaaata cactctggct 157800 gaagaaaata cagagcactt taggaaaaaaaataacacat catccacgtc ttcaattcta 157860 catcctaaat aaacagttta atcccatcacataacacagc aaagcctgtt atccgataaa 157920 aggagagtac taaaccctca atttaaggaccagttacaac tgattaaagt aaggatactt 157980 ataatttagt gtgtaagaat gtatgctgaaatataattag tgcctatgaa ttaaagcagg 158040 atggattagg ctaattcctg gaatgctattagagagaaga ccctaggttt aaaaaaaaaa 158100 gaaagaaaga aagaaagaaa agaagaagaaaaaagaatga tctttgtgtt tgcacagcag 158160 ctaagccgct taatgtccct ctaatggatctgaatgggaa gaaaggggag aaggggagtg 158220 gagccctctc catggcgtca gcccacgctacgcctgcctg gcactgcagc cctggaaacc 158280 tgggtacaag gcagaagggg ctgagttgcatgccctccgc tcacttccca gcttaattta 158340 tatggtaatt agagctctgc tgccagaagggagagcatct gccaaaagta aagtcctcac 158400 aataacaaaa ctgacattag ctgtgatgccgccaaagtgc tgactaagaa atttctttta 158460 tctccttttg aagccataaa gtgcattcatgttattacta gtcgggaatt tttaacagtc 158520 ttgcactgac agcaattatt taacacatgcacaaaacaat agagtttcac accacgtaat 158580 caacctttga gctccgtgac ttccaatgctctcactggct ggattttatg gtctggtagc 158640 tcctgaacag ccaagtatat aagccacaaaaacagtataa aaatgatttg aagcaaccac 158700 ttcttgtgcc cttgcagatt tatttaatgactgaggcaca ctccatgatc atgggccaat 158760 taaaagattt gtctgcccac cttattcgctgtgtttgact gctacaataa gtctctgcga 158820 aggaatcaag ctgaacgcat ggttgttgacctgtccctat atccaatttt tccaaaacat 158880 cagccagaat aagatttaag tattttagcaatctatttaa ggtggactat attactacct 158940 ttctcagtaa aagaattcat tcaaatattgtatttgaata tcaactctgc taaggcccaa 159000 aatagaatac tatatacaaa ggagaagaaaatcaagaaaa ctgctttcag gtatcatcta 159060 actgaggagt tgtaatcaaa taatagtgatagaggctgtg tgtgtttaaa gaactctcct 159120 ggaagtcaga tgtgggttat ggacaagtcaccaaagccct atgtctggtg tccttatctg 159180 taacaataga ggtagtgata gctatgccacctgcctctaa ggttttcatg aagatgaaac 159240 aaaaactgaa ggaacataga agaatagattttaaatattc tcatcacaaa aaaaaatgat 159300 aagaaggtga agtgatggat atatatttatcagcttgatt taatctttct acaatatata 159360 tacatatcaa aagaccacac tgtaccctataaatatattc aactattatt tgtcaaataa 159420 atttaaaaac tgcagtaaca agcactttgcaaactatgat gcactatata aataaaggaa 159480 taattattct ctgcaaatga taccaagagatttatttttt ttttttggcc actctaattc 159540 aaggatttta gtatgtaacc atcatagccatggagacctt gaaatctaat tgcatttact 159600 aatttactat gagttatcac taggaattccctaactcagt atcaagaata tagaaatcag 159660 aatatcaaag ttttaagaat tatgaaatttgtctaaaggg aacatatggt ttaaaataga 159720 tggctaaaac cagcaagcat tataaaacaaagtcaacttt taaaagactc aaattatata 159780 cacaaaatcc tcagtcacta tgtaagttcatgactttata catatttacg tgcaaagttt 159840 gaattatatt atgactcatt tttggccggtcatggtggct cacacctgta atcccagcac 159900 tttgggaggc gaaggcaggt ggatcacttgaggtaaggag tttgagacca gcctggccaa 159960 catggggaac gccgtctcta ctaaaaatacaaaaatcagc tgggtatggt ggtgggtgcc 160020 tctaatccca gctactcagg aggccgaggcaggaaaatcg cttaaacctg ggaggtggag 160080 gttgcagtga gccgagatca cgccactacactccagccag tgagactccg tctcaaaaaa 160140 aataaacaaa caaataaaat aaaaaataaaaaaaagatat atatatataa agactcattt 160200 ttaaaggcat ttttagtcaa ctagttttgagtattaaaaa catacttaat gtaagtcagg 160260 tacaatatag aaaggtcata atcaatcacataatctcttt cttagcccat ttatctgccc 160320 atacatctgt aacagagatg tgctgtcagtagggtttaca ttttcccagc aaacggaatc 160380 atagaacaat agggttttag agctctgtcttgcttttatg taggttgtac ttccctaaca 160440 tttgcatatg ccagcatttc acctattttcttaaactgca tgcaataggc atgccatgcc 160500 caccattttg aaaaggtact agctgcctacctgggattag tccttgacct ctacatcaaa 160560 atacacttct ggtcattgcc tttggtgtttaattgccttt ggtgttctct gtcagcctcc 160620 cctccatggc tgccaatccc catctgtaccccgtggcacc cagtacccaa ctgttcctct 160680 tctgcttgcc tcactctgct ccttctctctaaatgataac tcacactctg cctataaagt 160740 tttcttgcca aagtatcaca ttctactttacacactttac ccttcaaagc ctttaaatta 160800 ttgtttatat actcccatga gttatatttatttgtgtccc ctatataatt ttaaattctt 160860 atggacacaa atttaatgtt actatttttcacagtcaaat tcaatattac catattactt 160920 tcatatgcag aagtatgaca atgggtagacttcccacact atgcaaattc ctgctttttg 160980 acagatagtt aaatcttaat acactgttaggagcccactg actacgggac attgtttgag 161040 ttgctaagaa aattttctga aggataatcttaggccattg atgttgatca tgtgtgtttg 161100 cacacaacgt agagatagaa taaataaaaattaaaccagt gagcttacta cttcttgcta 161160 acattcaatt caaatattaa aagtagactcactcaagaaa aaaattacat tcctctatgt 161220 ttttaattgt cactgcttat ctaaaccttagatactaaga aatgaaggta agaagagatt 161280 agcagcaaac ttcaaaggta gtagcaggcgctttcgcaag aacataatta gatggtgatt 161340 tcattgacat tacttactca aaacacactctagagatgat gcagggctta tacatgaaag 161400 tatataaatg cttttgatgg atcacttcaagaaacaaaaa gcaggctgca atacccagca 161460 tatatttctc acaaatgact ccatatgaaaataagagaga aaaatagcac cttgcaaaac 161520 ttttcaatat tgtagaacag taaataaaattaacacttta gaggtagtaa aatctgtaac 161580 atatgtgaca aagagtatat gttttaaatatataaagaca gctttccaat acaaaacgaa 161640 aagaatacct taataaaata aataaatgaaaaaagataca aatgggcaat gcactgaaga 161700 agaaacacaa actgccaata aacaactgaaaacatgtcca aatttactag gaaccaaaaa 161760 aatataaatt aaagcaacta tatcatttttaaactctgag attggcagaa aaagaatact 161820 aacactatag gtatagcact ctagaatattcttagtgcaa gaataaatta cacaacctct 161880 ctggaggaca atttggcaac acacagccaaactctacaca tacactctca gcaattccac 161940 ttaagaatat atcctaagac aataattaaacaaatgctca ctctgtcatg gtttataata 162000 acaatattgg acattactag aaatgaacagagaaaggcct cattcaatag acacactcat 162060 atagtaccat aatgtattgt taagtgaaaaagcaggctat aaaatgtatg tagaatatga 162120 tcctggtttg taaaaagaca aaaaaaggaggaaggaagga gaggagaggg aaggagattg 162180 aagaagagag gaaaggagga gggggaaggggtgtctgtgt gtgtgtgtgt gtgtgtgtgt 162240 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgtgttgggatgg gggaaggtaa gcatttgggg 162300 tatgtgtgaa tagggaaaac ctcagacaggatcatgacca agtgttaact atgcttgtat 162360 gcataatttt tcttttcttc tttatatttttctagaatgc ctgaatgccc ccaaaagcta 162420 ttttcactac gaaaataaat ttaaactgtaatcacatttg catagccact ttataattaa 162480 agagaaatct tttaacaaat tctccatcattatcttcaga ggcagtcagt gattatttct 162540 agttgggtac aagagaacct ttcacattcacaaagtgtgt ttctagatat accaaacaaa 162600 aacaaaaaca aaaaaaccta aaacttgctgtgtgtccaca gagactcttt aaaaatattc 162660 tctagtactt cccagtggga agcgcctcagtggaggcagg gcctgaaacc tcattccatg 162720 tattagatac tggttggcca ataagaatgtgaagagttaa atacccttcc agaatactta 162780 atgtatggaa tgaactggga taaataataattacattagc aaatactgcc caaagaaaga 162840 tttctgagat attctaactt caggaatcaaatacaaactg agtcgacatc aagctcagca 162900 gcatataaga atgaagtaag acactctgccctgtgcttcc caaattcgtc ttcgctcgtc 162960 actcccaagt taagaaaagc ctataacagaaaaataataa aacaagaaag tttccatcat 163020 tttagataga cattgtgaat aattctacaaagacacactt ctcctacttc tcaatatgtc 163080 caagatgtga aattgagcgt attctttcttttccaagatt acacatatgg tttcaataaa 163140 tcttttattt caattttcat ttcttggccatcatttttta gatcaaatct aacaacccat 163200 gtcagactca ctttaaaaac acacagctagacacttctat gggacctctc tcttcactat 163260 ttttagactc tcctagaatc ttactaatgcctgtttcttc tataccccta gaaaattaga 163320 atgttgggga aatgtttact ttaaaaaaaaaaataaacca atgtatctga gtgctacata 163380 aatcaccttt ttctactctg aaactgccctgtcaatagtc actagccaca tggggcaatg 163440 gagaccttga aaggtggctg agtccaaatcaagatgtgtt gaaagtgtac aaaacacact 163500 ggatttcaaa tatatatata tataaaatattaattgtata tatatataat atacaattaa 163560 taatgtttat attaattaca tgtcacaatgatgtttgact atagtgagtt aaatataatt 163620 tcatctgctt ctttttgcct ttttgatgtgactagcagaa gatttaaaat tacttatgtg 163680 gctgcattgt gtttctgttg gatggtgctggtctggaatg ctagttcatc atgtcgagat 163740 tcttactgcc atttctgtaa ctctgcaaatactcagtatt ctctttaaat aataggtctt 163800 catcagtggc attactcaag agatttgctccctctaccac actcggtaaa acacctgcca 163860 ctagttactc cttttcccca aaattaacgtctgtgcttac tagaagcttt agctctactt 163920 taatctgagc actctgcact cctcgtgatcagtgttttcc ccagcggtgt tctgcccctt 163980 tgtgccacat acacttgagc agtggagtggcgttatagtt tgccaatgct ggtggatggc 164040 gtaactttca gtttggggtt caaactcgcttcccacaggg caaacacact aaagtgccac 164100 cacaattccc ttgcaaaact cattcattccctgaattctc ccagtgcacc aggaggaatg 164160 ttaaccattt tatacctgcc caaaagtggcctactgtatc agttaggatt gctttcataa 164220 aagaaaccca atgacctgcc tggcataaccaaggttggtt tattttccaa cttaagaaat 164280 tggtcagagg taaacagata agaggtgatgcaatagcttt atgatgggac aactgcccta 164340 gcctcctctg tctttccgac ctgcccttgtggtctcaaga tgcctcctga atgttcaggc 164400 agtgtatcta tgctccaact aagaagggaaaagatgaagg gcaaaaggca catgtcagcg 164460 gtttttcttt ttatcaggag acttctgcttttatctgact ggccagaact aacttatgtg 164520 gtcaccctag ttacaaggga gtctgagacagcatttttaa ctagaatttt atcagtaagg 164580 aagaacggta aatggatttg cagttgttaatgtgtaccat agctatattt tcacaaatat 164640 tattgcaatt ttgttagaga tacgaataaatgcctaggga taaggggatt aaagaaaaat 164700 ccaacaaaat tcacaatctt aatgttggatacaaagttgg gagaatggaa actttgagaa 164760 tataatgtct attatgtgga tcatttcattttattgaacg tccacttttt tgaatagttc 164820 ttacaacaac tttcagcatg tatctttgttgatagtaaaa gtataatcaa cctgccagta 164880 atatcttttc taaaagttta gttcatattaaatcaaatag ctgcttagaa aactttatcc 164940 accagtcaca gcctgctatt caacatggcctcactctact tttataattt tacttccttc 165000 tatttcctgt catccagagt ggatgaaagcagacctccaa tgtgaaaacg agtttcttcc 165060 tgagtcatct gcaaaattcc acactcctgtcacccatctg atccaatacc aaattcagca 165120 ttccactcaa atttctgaag cactgttgttgctaataaga accactgtct gtttttcaat 165180 cactgccact agaagatatt tttttaaaagacatagaaac atagcttcag tagttcacaa 165240 gtattaaggc ctacaccatg ccaagctgagaatgggagag ctgcactgcc tgaggatgcc 165300 aggccctcag ctgggagcac tcactcactcactacgtgat tcactcccaa acaagagccc 165360 tgcagaaagc ctgattcaag ttcttggttcagcaaaattc aagtttctaa aatgtcatat 165420 atctttatgg cattggcaaa aagtcagaactttctatgat taattcataa atgttactga 165480 tcaattctgt tccctgtaat ggcccaagctttccaaaagc tggccctttt ccacaaagtg 165540 tcttctttct gtaacaccca cccccatctataccaaatga aaccctactc attcctcaag 165600 gttcaattct gatttcacat tttccacaaagcctttccaa atcgctctgc tataactgtt 165660 ctctattttg atcaaatata gcactttatttctatttctc tcatgtcatt tatttctgcc 165720 ttatattttc gatcagtctc agcctctcccactagatcaa aagcttgact ctatactctt 165780 agcagagcat tttatttatt tgtttgcattgaattaaatc tggatttgtt ttgtctatgt 165840 gacagacaaa aagtaattgt gcaacatcattgaccacaat gtttttttaa agtctggtca 165900 caaattatga ctgtcaaaat aataatcagcccattgcttt ccattttgag ctccagagaa 165960 aattactaca aacatatgaa gggatgtcctatggccttga gcacagagga aagattgccc 166020 accccagtgt ctatacaagg ctttcactactccactatgc agcccatagg tccccatata 166080 aattcagcag aagaatccag ttacacaacgattcttgagc atctgcaaag gatgaataga 166140 tttcctgaac ccaaaaattg ggacacagaatctttgtgcc attccaggtg agttcccatt 166200 taaaatacag ctttgattgg atgcagtggctcactcatgt aatcccagca cttggggagg 166260 ccgagacagg cagactgctt gagcccacgagttcaagacc aacctgggca acatagtgag 166320 acctcacctc tacaaaaaat acaaaaattagctgggcatg gtggtgcgtg cctgtagtcc 166380 cagctatttg gggggctgag gtggaaggatcacttgagcc caaggggcag gggttgcggt 166440 gagtcaagat tgcaccactg cactccagcctgggtgacaa agtgagaccc tgtcttaaaa 166500 aaaatttttt aatggatcag ggagcacacttatttagtaa acatagcatg tattccaatt 166560 aataaactgt tcaatgtaaa aagttggggtgataagaatt aacattctaa ggaacaaaga 166620 gcagaagcta agaagggcaa taagaagggcaaaaaaaaag ttggaaaaaa atccacctta 166680 ttggcaaaaa aataatattc ttgcacatgtaggttgtgtg tgattataat gtatctcttt 166740 ttcttccttc tgccccaggt ctcagaggctaacgtgctat tctagagaaa catataacaa 166800 cttttctctg actggtttga aaaactgatgttgttctcac tagagggatg gtgcaaagct 166860 ttaaaaaagc tatagagtga tgaagggaaaatagatgtaa gtgccaggat cctgtttaaa 166920 ggatcaagga aggtctaact ccaagaatgtccacttaacg ttatattttc atttctacaa 166980 attctatctg gtttcatttc aaatctttgtcatattctca aagctcattt tatttcttta 167040 aatatattaa acagacatat tttacattctgaaatagacc actccaaact ctgcagtcta 167100 cgtggaactg actctatagt gtgttttttcaattaatgat tgctcatgat agtttatttc 167160 tttatgtgtt ggggatggga ggagtcctctaccgtggatt caaggccctt cagttttatt 167220 tgtgggaatt tattaaggtc tacgtttaaattattgtgtt cttccagaga gatttccctt 167280 tccttctacc aggagctggg attactgccaatccaagacc attttcaact gaacattcaa 167340 cttggcccta caggtggtat gaactcaaacccagagccta ccttcatgca ggcttatcgt 167400 tggtaactct catgggggac ttctcttttccattcctccc acaaccaaag cagagacagt 167460 tatatatgtc cccaccatgc cctctccagagcagattctt cctagtgttt ccactcgggg 167520 ttaaccattt ggtgtgctga ctctatgtgggagctctgac ttgaccttta ccctgtcttg 167580 ggctctgttt cctgtgctcc ctacacatgcctggttaaaa cccacacttt aggcctttag 167640 aggttaacag ataccacggg gaccaatgcttgttccaatg gtcacttacc cctcctgaca 167700 catagtgtct tgtcattcct accctccaaggaattccctt acatttctgc cagctcagcc 167760 atgcttttaa aactactttt taaaaaatattttatctagc atgttcaggc ttgctttccc 167820 aagcacggtc tcccttaaca tctagtctgccatatagcta gaactagaac tctcccgtcc 167880 agagatttgc acaggggctg taaacagtcccatgtggcat gttactaccc tgggtcaaca 167940 tccaaataac aggcttcatt tcaacctctaaaatcagtat aatgtttatg tatacatttt 168000 tcttttacct caatgctata ctgtttaaatatggtgtgag tgtgtatcac atattataat 168060 ttttacaata attattttat ataattatacttaattatat tcacatttat ttatttcttg 168120 aagcaagtac ctgtacttgt tccatgtaatcacacctaca gcacatggcc tctaccaatg 168180 aactttaaaa accaattcag aaagcaaaaggtaagttatg ttaaaaccta actagtcgta 168240 ttacctattt ctagagcctc tgctcaacaataagaattta aggaaaaata agtaatatta 168300 taaaacaaag ttactatcat aattgtcttttttttttttt tttttagatg gagtttcatt 168360 cttgttgccc aggctggaat gcaatggcacgatttcggct cactgcaacc tccccctccc 168420 gggttcaagc agttctcctg cctcaccctcccgaataact gggattacag gcatgcacca 168480 ccacacccgg ctaattttgt atttttagtagcgatgaggt tttgccatag tggtcaggct 168540 ggtctcgaac tcccaacctc aggtgatccacccacctcag cctcccaaag tgctgggatt 168600 acaggcgtga gccaccgtgc ccagctcataattgtcattt ttaaggtgcc tctcaagata 168660 tggcacatta ttattcagaa acagcacaatgcctacacct tctataacat atcatctgca 168720 ttatcataac acatgtgaaa aaccataatccagaaccaaa catctttaat aatttttaaa 168780 atgtttacag tataggtaga atacaaagtatatttataac ctctcttgtt ccaaaataaa 168840 tttgaaactg aaacctacta atatatttataaattgatta taaatgatag ttgaaacaaa 168900 gtaagttaat ggaaaaatat ccaactatccttaacatttt catctcaaac attaggagaa 168960 atatctaatc catgcagggc ttaaaacctagatgatgggt tgataggtgt agcaaaccac 169020 catggcacat gtatacctat gtaacaacctgcactttctg cacatgtatc ccagaactta 169080 aagtaaaatt ttaaaaaata ataaataaataaataaaaac attttcctct caaagaactc 169140 cagctaatta tgttttaaat ttctcataaattatattgat ctatgtagtc aataactttt 169200 aaaaggttac tgggtcattt attcattgcaggataattca tgatcatcag cgaaatacct 169260 ataactgtac tacattctca actattaaatgataatattc cattgcaagg agttttgtta 169320 aggtttctgc attaaatgat gtagaaatatataattctga aaattttatg ttctacttgc 169380 ttgtttcaaa atgggcctgt gattaagagaacaaattttt taacgtttct ctgttaatgt 169440 acaaatctca ctctccaaag tttacaaccaaatgttttgc atcattacac acacctgcca 169500 ccacctacag aaagaacaaa agttgtatactctaaatacc aattgtatgt ggcagtgtta 169560 ttattttatc cacatttcta aaacctgttacaagtgaaaa aaagattaaa ataatttctg 169620 cagttctaaa caaagacacc aaagtataactatttttcca attcggacct tcattaatgt 169680 acgaatgcca aagcacacta gttggtcttcctatttaacc acaaacatgt caggtctgtt 169740 tggagtagtg ttgaatgtgg aaaagatgtatatccggttg gtaacagctt aaaggtttta 169800 gaataaagcc tacatttgtg ggtggccacaaatcttagct aaaaattggc tgaactatac 169860 ggacgtgact tagagaggtt ggatttattgaaatgttcac aggctgaggt gtcagaagag 169920 ttggccttct gattatttct gaaaaatcatttagctgctc aagactttgg tttctaatcc 169980 atggaactgg cccaatgacc tccaagcccctttagctgtc acattcaaga attctgaatc 170040 tatagcatgt gtaacaattg aaggtagactttccacccca ttctctaagc agaaggaaca 170100 ctccagtctt ctgactgggg ttcatatatgcttatatcag gttaaatgac aagagcatgt 170160 cgaaataaga atcaaaatga gaggatttatcatcctttgt cagccacatt cttatcctca 170220 atgctcagca acagggctta gagtgctaaacattttgatg caataatgac aatgcagttc 170280 accaaacaag gccaagctca gtggtttccaggaaactgaa aagagaaaga atgtggatag 170340 aaatagtaga gacttcaaat gatgaagaaaatgaatgaag ttcaaactga gaatcaaaaa 170400 gagacagaaa aaagacctaa aaagtgtggtcaatgcattt ggaatgacta gaaccaggga 170460 aagtttttcc taacaatact aataaaacatctttgagagt attttatcat tagtgggatc 170520 aatgggataa ggtcagattg attattttttatttaaaatg caaaaactaa aatggaattc 170580 ttttgatttg tgaaagatag gatcattatgcaacttcatc aaaggtattt ttacctaacc 170640 ctagaagtca aaaatgttct ttattttttaaaaaaaacaa aaaatcttta ttgtcacagt 170700 ggaacactca cacctctgaa aaatgagacaaatcatgaat tattgttact attcatttaa 170760 attactcaaa aagtccatca ttatgttaatggattaaaat atctttaaat aagagaatga 170820 tataataaaa ttgccttaat tatggctcccctttctttag gaataaaggc aatttaatag 170880 aaagtagaaa attatctaat aaaggtaagttacagcacat attctatttc ccttcagatg 170940 gttgttagta tatatgcgcc aatatacgaatatatatata taacatagtt gataaaaatc 171000 tatcttaaat gtttattttt atgtttcgtatcatatattt tcaattaaaa gtcatatatt 171060 aatcaggtgc agtggctcac acctgtaatcccagcacttt gggaggccaa ggcaagcaga 171120 tcacttgagg tcaggagttc gagaccagcctggccaacat ggcaaaaccc catctctact 171180 aaaaatacaa aacttagccg ggtgtgctggtgcatgcctg tagtcccagc tactcaggag 171240 gctaaggaag gagaattgct tgaacctgggaggcagaggt tgcagtgagc tgagatcatg 171300 ccattgcact ccagcctagg caacagagtgagactcggtc tcaaaaaaaa aaaaaaaaaa 171360 aatcttctaa aagtcttcta ttgacaaagtccacgtactt gtcattttta tcatcctacc 171420 atagaattaa atgggttaat attatataagttgctttgac attcccttct ttgagaacat 171480 ttatttcctg ggttttttgt ttggttggttgtttggtttc ctcgggcatt attactttga 171540 aaatattcct aaaagtagaa ttatacaggtcaaaaatatg aatcatatta tagctgttgt 171600 tacatttatt gaccaattgt tttccaggagaaaaaataac ccattcaaac agctaccaat 171660 aatgtccaaa tatcgctgtc ccaatctttacaccactctt actgacacat aatgatatca 171720 catttgtcat tcttttggct ggcctaacgggtgtatatag ttcctacagg ttgtctgaat 171780 atgaatttta attgctgata aggctgaatattattccttg tgatgactca ctctattttt 171840 ctaaatatat aaaaaccact tactgagatacaataagcaa tgatctcttg tacatgtaga 171900 tgctttccat atttttaaac attaatttatttctgtttat agacacaaca tatgtatatt 171960 acagacagtc tgagatataa acaaaagcacaagaataata taaaaatcac ccataatccc 172020 aacacacttt tccctttaaa tactgtgtgttacaaaaagt taatttcctt tctccgcctc 172080 cccatccatc ccgatccccg ctaaaagtaattactgttaa gcatttggta tatgtatatt 172140 gttccaggac tttcctatgt ttctttctataaaaatagaa tcacactgat cattgtcttc 172200 tgcaacaggc tctttttcct taatactctatcacagacat cttttcaaga tacatagacc 172260 tatctcattc ttttgatagg cagatggtattctaatctgt gtacagtaag ttatgaaact 172320 gggtcaaagc aaggcaaaag ggtcccagctgatatctgag gtatatacat tacaattttt 172380 cagttacggt ctcataggta actgccaccagttcccctgc ccccataggt atctgccacc 172440 agttcctctg cccccttgat ctttcactgcaatcacccaa cctctcctca cccttgcctc 172500 aactcatcaa ccctcatctc cagctttggtggactgctga gggctgtcag agaaaccttt 172560 cagccttcct gactggctcc atttcgcagcagggtttcca acctcccctg ggctacctga 172620 ttgtttacca ttgctttttt ctcatcttcactagctctat tccagtccct tcctaccctt 172680 cttcaatccc cactctaccc tcccactctgaccatacgga gaggtcgcta atttacagag 172740 taagtagaga acattcaaac atggcctcacccaccttctc ttcatctctc atcctgcctt 172800 tcttggccca cccttccttt ctttcaccccaagtcaggaa aagggtacct tggttcttct 172860 ccaaggctta cccctccatc ttcaatgttgccctccatac gatgtccttc cccccttatc 172920 ctggaaacaa gcttatgtaa atgccatattaattatgtta ataataaaaa tgtatatagc 172980 acttaaaatg tccagaccct cttctaagtaccttatatat actaatttaa acttcataac 173040 agccctatga ggtgagtgtt acagtaattgtaattatctc cattctacag attagtgaat 173100 tgaagcacat tgggctcagg ggacctgccaaggtcacaca gctactaagt gagattcaaa 173160 ccccaagcag tctggccctt aaccaccaagccccctatat aaagcttata taataggacc 173220 agggaaatga tttagagcag agtggagttagaagactgga tgggcagaga agcctgtaga 173280 gcgccccaac caacagaaaa ctactctcttgtttgtctgg tttgaagtac cagagacaaa 173340 catcactggt atgggaaatg atggagagactgtctgtggg ggctgtctta tggtgcctat 173400 tctatgaaag cggatactcc cattatctcttcattctaac atctcagatt gtctccatac 173460 aagtagttaa cctggtggaa aaactcttatccatggctaa agccactaca cctaatacca 173520 tgggaaccaa ctaggcaagc tgcaggtggctcagcacaaa ctccctgaca tgtgaaaaga 173580 gctcaaagca cagctcctcc acctactcaccatctacagg ctgcctgctt atgcctgaga 173640 cactcaccta aaccatccag gcaccccatgatacttcaac actctctcta cccagccagt 173700 gtcactccag tctactttga atacttagtcccagacagac catttccaat gagataattc 173760 taactttctt cctcaatatt taccagaaatacgtctcttt ttggcttccc aatcaccatg 173820 cattttcctc ctccactata aagaatcaaattaacattta acatgttagc cctttaaatc 173880 tctttgggaa gttaccatct gtgatttttctaccaaacca ctccccttcc ctcctacccc 173940 aaccaacaca cacaaaacac actagtcccttctttgctgg gctaaataaa tacatgtagc 174000 cccattaaca atccttcata tgacctggttttacatctct tcctcatctt ggtcactcta 174060 atatctaaat atgatccatt ttgcttacttctctcttaat gtgtcccaga gcagctgtat 174120 gacactgttg actcacacta aggaaatcctacagatgtgc ttcacacatg ctacttccag 174180 cacccaaggc tcatccttcc tccactctcagagctgcttt tcaaggcccc tatatgccta 174240 ctgagtttca tattaaatgt gtgagcttcaacctcattcc attgtgtcta cttcccctag 174300 ctcctaatag ctaaatccac tgatgccaacactaccacct cctacagact aaatcaaatc 174360 ccacttcctc aaccagtctt gaccaagacaaatgtgtatc agcagcctca gcctaaccct 174420 gaaacacaga attaagctac ttttccagcatttactgaca aatcaatcct agatgcaggc 174480 gtttccagta acccccaggg ttcctatactgccccttagc tcactctttc ttctcccatc 174540 gctaaaacag gatatgggtt tgatccctagatcaccaatt cagtggccac agattacctc 174600 ttccaccagt aatcctgaaa ggatgttccattgcaggagg cagaataagg caaaactgca 174660 aagttggcca tttcctatca ttcagaaaccacattctctg gttggagggc agtagactaa 174720 ttttgtatat agaaaagaca atctcgcattctcctaaaaa gtgttactaa actttcaaaa 174780 gtaactttca atagaagatt taatccaattaataacaaat atttaggaaa cacaaactag 174840 ttttagatac tatatattac aaataacatcacttcttatc aaaaataagt gtgccaagaa 174900 tctctggcaa aagaacagag gtcatcttgcactactctct tgattttaaa gcaatgaaat 174960 gaagtctctc agacagtggt ccccaaccctttaggcaata gggggagggg tgggggaggg 175020 gtatggtttt tggatgaaac tgtcccaccttaggtcatca ggcattagat tttcacaagg 175080 agcatgcaaa ctagacctct ggcatgtgcagttcacaaca ggactcgcgc tcctatgaga 175140 atctaatgcc actgatctga caggaggcagagctcaggta aagcaagaga tggggagtag 175200 ctgtaaatac aaacgaagcc accctcacctgctgctcacc acctgctgag cggcccagta 175260 cctggtcctt ggcccaggcg tgggggacccctgctctaag aaactatgta atttattcaa 175320 ggtcacagag caggctatta acaaaactgaaacaaggact caggtctcca gattcccggc 175380 tgaaataata tctagtcatc tatgaggtacagtgcgtttt cagagtttga aacacacagt 175440 atcttcccct caacaggtca cgtggtacacaacatgaatc cacaattatg aaatcagtta 175500 tgaatctaca gttatgaaac actaagcaatgaaaacatac aacagggcca ggcatggtgg 175560 ctcaagcctg taatcccagc actccaggaggccaaggtgg acagattgct tgagcccagg 175620 agttagagac aagcctggga aacaaggcaaacccccgtct ctcctaaata taaaaaaatt 175680 agctggtcat ggtggcacac acctgtagtcctaactactc aggaggctga ggtgggagga 175740 ctacctgcac tccaacttgg gcgacagaagtgatactctg tctcagaaaa aaaaaaaaaa 175800 aaaaaacata aaacagtaca gatcaacattcatgggcctt tatcatgcag acaacagcct 175860 ctgaaagtgt tgactataaa acacaaaactatgaaaatta aatttgtata ggggaaccca 175920 aacattttgc tcatgattac tgattataacaagtttatca cactttaatt cacaaaaaaa 175980 gacattaatc atgaaaagga aatcaagtctcagaaaggca gtaatagcat agttattaaa 176040 tgttaaccat ttgaatccta cctcctccacttactagctg tataacctca aggaattagt 176100 caacctctct ttcgctccat ttcttcatctataaaatgag gactgtggta tttatctcat 176160 ggagcagttg caatgattaa atactttagtatctgtaaaa tacttgggac agtgcctggc 176220 tcatagttgg tattcaataa ttgctcttatctactcatgt attattcttt tccacttgga 176280 taacaaccaa ctcttgacat ttgtcatatccctttaagtg tagccaaatt ccataaggca 176340 ctggaatgac atacgatttc ttaaaggaccctggttttga gccaaaaatt aaataatggt 176400 agagatgtgg tagaaagtaa cagctacatgttcaaggtat gtgataaatg gccaatgtgt 176460 tagtctgtct gggcaacaaa ataatggtattttgggtggt ttataaacaa cacaaattta 176520 tttctcacag ttctagaagc tgggaagtccaagatcgaag agctggcagg tttggtgcct 176580 agtaagggcc catttccttc ttgatagggatgacttctca ctacgtcctc acatggtaaa 176640 aggggcaaat gagtcccctg gggcctcttttataaagtca ctaatttcac tcacgagggc 176700 ttcactgaca tgacctaatc acttgcccaaattccccacc tcctaatccc atcaccttgg 176760 ggactagggt ttcaacaggg attctgaggggtcacaaaca tccaaaccac agcagtcaga 176820 aatcaggaga atggatcatg gggagtgacaggcttagccc gccttggatt ggcagatatg 176880 gagtgagagg aagtaccaac agttttcaaaaccaatcaaa ccaaaacatt tcatttatca 176940 catcgaattc acaatgtact atcgttaccactacaatgac tggccaagag cctccttaca 177000 cttccctacc attcctactt cttttttttttttttttttt tttttgagac ggagtctcat 177060 tctgtcaccc aggctggagt gtgcagtggcgcaatctcgg ctcactgcaa tctctgcctc 177120 ctgggttcaa gcagttctcc tgcctcagcctcccaagtag ctgggactac gggcgcacac 177180 tgccaagccc ggctaatttt ttttgtattttagtggatac ggggtttcac cttgttaccc 177240 aggctagtca cgaactcctg agctcaggcaatctgcccgc ctcggcctcc caaagtgcta 177300 ggattacagg cgtgagccac cgcacccagcccattcctac ttcttctact tcatcccatc 177360 aacagaatca gagaaatctc tctgaaaatggaaatagagg tctccctgaa atataatgcc 177420 catgagattt ctcatgcaaa gcaaataaaaatgagctata aaaaaagcac gatgtattca 177480 ttccctttag atctgctgcc taactctcattagggggcct gagctcctgt aggctctgtg 177540 tcagtgctgc tgcagtgggc ctgcaggcagtgagggcagt tcagctgggc ttgattacaa 177600 ggtgtgaaga tgggattgtg gcacactctcaggggctctg aagggcatca cattttaaaa 177660 tcactcacct gatgtttact ccatggtgcagcatattaag tggaataatg agagaaggga 177720 gtgactggaa acaagaaatc ctacaaaaaccaggttataa gtggctaagg catgaggtga 177780 caacactgag agaagggtga taaaagtagggaccaaaaaa aaagattttg agtcttaaga 177840 aactcaggaa gaatatggca acaaactagtcacattggtt aaaactcaaa gaagcttgaa 177900 gatcacccaa gatgacagaa tttagtaggagaccaaaagg ggttactgca ggtaattacc 177960 gcaagaccac aatcacaagc tcagccttgacatgtttaat tttaggtaaa atccaggtat 178020 ccagctgaaa acattctatg ggtagtgggaaaatcatagt atgacagagc aattctaaga 178080 ggttcagagc atcaaccagc tcacaacttagttgctttaa gagttttcac tcatcaggaa 178140 tgacatgtct ttatgttgag caagaaaaacatgaagtagg ccaagaaaga tttggaatgc 178200 attaaaatta actagttact aataatatattagtaattac aaacaaaaag agaatttaag 178260 ctataagttc aaaacatagg gaagattttagggagaacta ccaaattttt ccacgaaaaa 178320 caaatatttg tgtcactgtc agaatattaggattatggat tattattctg acttcacata 178380 gtatcttaca cactatctga agtcagaatatgttttatat gttatttaat gttttatatt 178440 atataaattg cttaaattaa gaataatatcaaagtcctac gaattatatt tttagctttt 178500 attcacattg ttactgacaa attcacatggctgatttaac acatgagaac agtcataaaa 178560 cagtcaaaag agactgaaac cttttttggtacaagtgttg gttacaatta aactttaaat 178620 aagagagatg gacgggtgcg gtggcccacacctgtaatcc tagcactttg gcaggctgag 178680 gtgggcaggt cacttgagcc cagaagttcgagaccagcct gggcaacata gtgagaacct 178740 gcctctattt ttttcttaaa aaaaattaattaaaacaaaa agacttacca cattttcctt 178800 catcattttc agcttttcat caatctctctcagccgatta tgcagatcgc gttgctcttt 178860 ggtgttccta agtatctttt ctcctggagttacttccata tttctttcac catcacggtc 178920 aggtttctaa tttaaagaaa aaaaaaaaacatcattcaca ggctggaaat gtgctgtcac 178980 ataccacctc aaagcaactg agccttgagggttagctttg cttctcaaac aattatttct 179040 tcccttcaaa acaatatgta taacgctttaaagtcttccc attttaggag gtaaatgtcc 179100 aactgtacat ataacaagta ggaaaggaacccaacttctg taatcatttg ggaagcagaa 179160 ggagacagtg aaaacagctg gagccaggaaacaggaacct gggttctggt ccctgctctg 179220 atacttacca gcttgagacc ttggacttttctttggactt caagtttatc atgtataaat 179280 aagaaggaaa aaaccctgcc catccatattattagaagca gctgtgtaat aatgggaaga 179340 aaactggatt tggcaaggtc aggagttttgaatctttgcc ctgcctggtt cctgactaga 179400 ctgacctgag ggaaatgaat taacctctctgagcttcagt ttcatcatct gtaaaacagt 179460 aacagtaata atagatctag gctaaaagtcatcacagtat gtagtaggca agcaacaaat 179520 cttaatgcca aacaaatgcg agctgaatatgtcctttgaa aagactaagt attacataat 179580 gaaagtactt cattattgtc tgcaatatttatattgacag aaccatattt tcccaaagtt 179640 aatgttattt ctgggtgata aaaatgggcatttcttgata ggaatttggt cctgtatcta 179700 agattaaact tcctttctca catacatctgctctgtgttg ccatataagt gatgggcagt 179760 aatgacattt ttgaaatcac tcaagccataatcctgctac tccctcattc cttacataga 179820 gatggtcacc aggaatacta tctagttgccaatccctaat acctctctaa tcaaggcact 179880 tgtttctttc tcaccgtctt agttaagatatcctcatcat tcaccagcag tttttaaact 179940 ccagtgagct tcagaatcac ctccagagcccaggaaaaaa tccgaaggac cagcctctac 180000 ccaggaggtt ctgattcaaa agggaaggacaggacaggcc tggtggctca tacctgtaac 180060 cccaacactc caggaggtca agtcaggaggatcccttgag cccagaagtt agagaccagc 180120 ctgggcaaca cagtgagacc ccatatctacaaaataaaaa ataaaaaaat aattagccag 180180 acacggtaac acctacagtc tcagccacttgggaggctga ggtgggagga tcacttgaac 180240 ctgggaggtc gaggctgcag tgagctgtgatcgcaccact gcactgcaga ctgggcaaca 180300 gaatgagact ctgtctcaaa aaaaaaaaaaaaaaaaagga ctaaggacta ataaactgaa 180360 gcacacctac ttgagaattc aagaactactgacttaggtt gctgccagag cctcctggct 180420 ggtcttgatg ctaccagtca ccctgttgattccattcttt gcagggaaga cacagaacaa 180480 ggtgcaaagt attcatgctt taggccctgccaatctcttc atctcatttc ccactgcttg 180540 agactcagac tcaaatgtgc agcaatattcactactacca gttcccccaa ttccaaccct 180600 ccaactcctg agactcctac ccattttgggcccagttcaa gcatatttcc ataatattcc 180660 ctgacatacc cacaaaggac aaattattctctcagccacg ccacatgctc ctttattgca 180720 cttaccacac tgtcaccacc tagacagtgacatggctgac cctattctct gcatcccaat 180780 gcaaactata atcctgaagc tgtgtaagcactggccaata gactgtaatt tgggttttcg 180840 aaactttcat caaactagca atgaatttcagtaaaattct tattgtatac agttctacca 180900 agcaaattat taagagtgcg tcaatttcaagaagtgatta ttttatattt ggcttcattt 180960 ctcacttctg gtagctttca atctctagaagcatcctgat atcgtttgga tctctgtccc 181020 taccaaatct catgtcgaat tgtaacccccagtgttggag gtggcgcctg gtgggaggtg 181080 attgggtcat gggggtggag ttctcatgaatgggttagca ccatccctca gtactgctct 181140 catgattgtg agtgaatgag ttaccatgaggtctggttgt ttacaagtgt gtagcacctc 181200 cctcttctct ctctcttcct cttgctctggccatgtaaga agtgcctgct tccccttcac 181260 cttctgccat gattgtaagt ttcctgaggcctccccaaaa gcagaagctg ctgtgcctcc 181320 tgtacagcct gcagaaccgc gagccaattaaacctctttt ctttataaat tacccattct 181380 caggtatttc tttatagcag tgaaagaacagactaataca catctagagg ctgaattatt 181440 atcagaaata ttttttaaag gaaatctaatgttaggggga agtttggaaa aaatggtatc 181500 aaggtctctt tgcaatgcta agcagtcagcattttataaa ctttcatgaa gatacaccag 181560 ctatgaagta ggacacatgg taatctgcaaaacaccccgg atttcatttt taaattgaaa 181620 ttattttaat aatctacaac tatgattttttctaaatctt ggggcaagca aagagaaggc 181680 acagaaagaa aaaaattata agttgcacttaacaacattt ttaaatgttg cttaatcaaa 181740 accaccactg tgaaaatgaa caaaccaccaactgagagaa aatgatcata acacacaaat 181800 ctgacagagg acttgtatcc agaatatgtaaggaactcct acaactcgat aataaaaata 181860 atcaaataga caataataat taatcaaatgacaatcaaat tctataaaga cttgcacaga 181920 tacttcataa aagctatgtg catgacaaataagaacatga aaacctcctc aacatcatta 181980 gtcactgggg aaatgcaatt aaaccacaatgagacactac tacacagcca ctagcatggc 182040 ccagatgaaa aaaactgtga acaccaaatgttggaaatgt gtgaaacaac agggactctc 182100 ttacattgtt tgcaggacta tacaaaggtacaaccacttt gaaaaacagg ccatgtcttt 182160 tttttttttt ttttttttga gacggagtctcgctctgttg ccaggctgga gtgcagtggc 182220 gcaatcctgg ctcccggcaa cctctgcctcccgggttcaa gcgattctcc tgcctcagcc 182280 tcctgagtag ctgagattac aggcatgcaccaccatgcct ggctaatttt tgtattttta 182340 gtagagacag ggtttcacca tgttggccaggatggtctca atctcttgac cttgtgatcc 182400 acccaccttg gcctccaaag tgctgggattacaggtgtga gccaccacgc caaaacaggc 182460 catttcttaa aaagttaaat gtttgtcgatcatgtgatcc aaaaattcaa ctcctatgaa 182520 tttatcaaaa agaaattaaa atatataactaaagccttgt acacaaatgt tcatggcagc 182580 taaaaactga aaacagccca gatgtccatcaatagagtat gataaactat ggtgagcacc 182640 tgccacagct gggacaatca gagtgtttctccttgagctc catcttctgg ttaggtttag 182700 tcaaagtgag gggagagtac aggacagaaggagagagagc ttagggtatt ttttcccctt 182760 cccctaatgc ttttgcatca cagtttgggcagtgatgtat ccctgagatg tgctgagttt 182820 tcttagattc aactgcatat tcatgctcaaagactaacag aggacacact tagagccact 182880 ctactcagaa ggttataaga caggaaacacagcaggccag tatcaggtat cccttttcaa 182940 tgggagtgtt tgctacagtc catgccaaaacaatataggt ataaagagca aaacagctat 183000 aagctgactg tcttaaaatc ctcatatcctacaggagcca ttatctcttg aaatggcccc 183060 ataggcacac cgagtccaag gacccagcaagaaacgtgcc cagccacaag aatcactgca 183120 gtcagagatg gccaaaatac agcttctccatcaggtcttt ataattctcc caatccaggg 183180 atcattttaa gcataagcaa agtcgcctcctaacatattc agcatgccaa cttttctcag 183240 attgccaagg aaacagagtt aggaaactaattaccgctca agcgctcacg ctgagggggc 183300 tggggttggc taaccctttg cccatgtcctctattattac aacttcagat ggccagttct 183360 tctcccacaa ttccagctct tgctgagctcccacacactt tttcttcccc catccacggt 183420 ggctagtctc tcggtgtccc aacatccactgttagctcct ttaaatctat ataaatagtc 183480 ccttcatata attatcttcg aaatccaagctaggtattcc ttctgtttct tgtcaaaaac 183540 tctaacatag atatatacat agatagatagatagatatag atacagatat agatatacac 183600 acacacacat atatatacac acacacaaatataattatat acatatatat gtgtgtgtac 183660 atatatatgt gtgtatatat atatagtctgtgtgcgtata gagaaataga ctggaaggaa 183720 atttaacaat aattaacttg gcaaaaatcctgatgattta atcttatttt tcttattttt 183780 ttgtagttcc tgaatttcct acaataaacatgttatcaat ttcataagca gacaaacctt 183840 tttttttttt tttgagacac ggtcttattctgccacccag gctggagtgc agtggcacaa 183900 ccacatctca ctgcagactc gacctcccaggctcaagcga tcctcccacc tcagcctctc 183960 aagttcaagt ggctgggact acaggtgcacaccacctcgc ctggctgatt ttttttgtat 184020 gaagaagaaa tgggtttcac tatgttgcccgggctgattt tgaactcctg ggctcaaggg 184080 atcctcccgc ctcagcctct caaagtgctgggattacaga tgtgagccac tgatttttta 184140 tttttaataa acttttctgc caaagccatttttgtcctgc tctgacaatg tgactctcca 184200 tacctaagag gtatacaccc gaaggttggattctagcttc caatccaatc agctgtgcac 184260 ttttgaggaa gtcacctcct ctttctgggtctcagttact tctgagaaag ttatttcccc 184320 ttctaatagc ttatgagtct ataattctctacagttagac ccaaaggata tataacagct 184380 ttcttcagtc atggcctccg atggcataaccatatagttc aaatattcga aaaatactgt 184440 atgatgaaag cagaggccaa gagggggcaagaaaaggttg ccactgatgc tgatgaaaat 184500 tgcccagctg caaaaagctc tgtggtgccaggaaagattc ttttttcaca acaaagtgcc 184560 aaccagtact atctgtcctc gtgatcatcaggaaatatct tgatttctca tagtagaaat 184620 atttaatcag attatctgac caacatatcatttctgtgtt tctgggtcat acaggaatgc 184680 cttcatggag aattcaataa aagctctcctccatatgcca caagaagaga actgtccctg 184740 aggccaagat caatagatac ttgttgatttgactaattgg ttgccaagag attctgagtt 184800 tatccttaga aaaattatct gtggtgttgactaggccaaa agggatcttt gaagcaaaat 184860 aaactcattt tcaaaaatca aaaacagcaaattacccgca gcttcgtaag ttatctgata 184920 tgcaaataag gcatgcgtta ggattgggaaaatacaaacc tgcctaaata aagacagctt 184980 gtcctatgtt ttcgagacaa tgctatacatttctgtaggt tcctgctatt tcttgtcatt 185040 agatggatcg tgtgtctggc agtaccaaaagcagggaaaa agatacttca ggcagattgt 185100 ttttatcacc ttggaataat gccataaaatgaaggaaacc ttgactacat cagaagctta 185160 acagaggaaa gcaaagaaat ataaacttcacagtaatctg taaaatggag gtggcaaggg 185220 cagtttccca gatttaactg gagatctaacagattggcac tggaggtaaa gagctctgaa 185280 gtaacttctt gtttgttcct caaacccagaaatgcatgct cctgtggctt ctatgggttt 185340 tggaaatgag cagatggtca ataagatttctgtgcctgtg actagcatac atttaaaaaa 185400 tcctaaattc ctgccctcaa ccaacacacacctaagtaca tttggggtca agtagagtcc 185460 atggccctta ctggaactat acaaatccctggacccaata ataaaacatc tttcagaatt 185520 acatgtcaaa caatggtctg tatttcctcaattttagcta atattgagac aggaagacat 185580 caaaaaaaaa ttaattgttg ctgtcagcaataatagcatg atcttggcaa gaaaggagat 185640 caatacaaac tacaattttc ctcaatcaaatgtttgctaa ttttttctca agctt 185695 2 1170 PRT Homo sapiens 2 Met Gly LeuAla Trp Gly Leu Gly Val Leu Phe Leu Met His Val Cys 1 5 10 15 Gly ThrAsn Arg Ile Pro Glu Ser Gly Gly Asp Asn Ser Val Phe Asp 20 25 30 Ile PheGlu Leu Thr Gly Ala Ala Arg Lys Gly Ser Gly Arg Arg Leu 35 40 45 Val LysGly Pro Asp Pro Ser Ser Pro Ala Phe Arg Ile Glu Asp Ala 50 55 60 Asn LeuIle Pro Pro Val Pro Asp Asp Lys Phe Gln Asp Leu Val Asp 65 70 75 80 AlaVal Arg Ala Glu Lys Gly Phe Leu Leu Leu Ala Ser Leu Arg Gln 85 90 95 MetLys Lys Thr Arg Gly Thr Leu Leu Ala Leu Glu Arg Lys Asp His 100 105 110Ser Gly Gln Val Phe Ser Val Val Ser Asn Gly Lys Ala Gly Thr Leu 115 120125 Asp Leu Ser Leu Thr Val Gln Gly Lys Gln His Val Val Ser Val Glu 130135 140 Glu Ala Leu Leu Ala Thr Gly Gln Trp Lys Ser Ile Thr Leu Phe Val145 150 155 160 Gln Glu Asp Arg Ala Gln Leu Tyr Ile Asp Cys Glu Lys MetGlu Asn 165 170 175 Ala Glu Leu Asp Val Pro Ile Gln Ser Val Phe Thr ArgAsp Leu Ala 180 185 190 Ser Ile Ala Arg Leu Arg Ile Ala Lys Gly Gly ValAsn Asp Asn Phe 195 200 205 Gln Gly Val Leu Gln Asn Val Arg Phe Val PheGly Thr Thr Pro Glu 210 215 220 Asp Ile Leu Arg Asn Lys Gly Cys Ser SerSer Thr Ser Val Leu Leu 225 230 235 240 Thr Leu Asp Asn Asn Val Val AsnGly Ser Ser Pro Ala Ile Arg Thr 245 250 255 Asn Tyr Ile Gly His Lys ThrLys Asp Leu Gln Ala Ile Cys Gly Ile 260 265 270 Ser Cys Asp Glu Leu SerSer Met Val Leu Glu Leu Arg Gly Leu Arg 275 280 285 Thr Ile Val Thr ThrLeu Gln Asp Ser Ile Arg Lys Val Thr Glu Glu 290 295 300 Asn Lys Glu LeuAla Asn Glu Leu Arg Arg Pro Pro Leu Cys Tyr His 305 310 315 320 Asn GlyVal Gln Tyr Arg Asn Asn Glu Glu Trp Thr Val Asp Ser Cys 325 330 335 ThrGlu Cys His Cys Gln Asn Ser Val Thr Ile Cys Lys Lys Val Ser 340 345 350Cys Pro Ile Met Pro Cys Ser Asn Ala Thr Val Pro Asp Gly Glu Cys 355 360365 Cys Pro Arg Cys Trp Pro Ser Asp Ser Ala Asp Asp Gly Trp Ser Pro 370375 380 Trp Ser Glu Trp Thr Ser Cys Ser Thr Ser Cys Gly Asn Gly Ile Gln385 390 395 400 Gln Arg Gly Arg Ser Cys Asp Ser Leu Asn Asn Arg Cys GluGly Ser 405 410 415 Ser Val Gln Thr Arg Thr Cys His Ile Gln Glu Cys AspLys Arg Phe 420 425 430 Lys Gln Asp Gly Gly Trp Ser His Trp Ser Pro TrpSer Ser Cys Ser 435 440 445 Val Thr Cys Gly Asp Gly Val Ile Thr Arg IleArg Leu Cys Asn Ser 450 455 460 Pro Ser Pro Gln Met Asn Gly Lys Pro CysGlu Gly Glu Ala Arg Glu 465 470 475 480 Thr Lys Ala Cys Lys Lys Asp AlaCys Pro Ile Asn Gly Gly Trp Gly 485 490 495 Pro Trp Ser Pro Trp Asp IleCys Ser Val Thr Cys Gly Gly Gly Val 500 505 510 Gln Lys Arg Ser Arg LeuCys Asn Asn Pro Ala Pro Gln Phe Gly Gly 515 520 525 Lys Asp Cys Val GlyAsp Val Thr Glu Asn Gln Ile Cys Asn Lys Gln 530 535 540 Asp Cys Pro IleAsp Gly Cys Leu Ser Asn Pro Cys Phe Ala Gly Val 545 550 555 560 Lys CysThr Ser Tyr Pro Asp Gly Ser Trp Lys Cys Gly Ala Cys Pro 565 570 575 ProGly Tyr Ser Gly Asn Gly Ile Gln Cys Thr Asp Val Asp Glu Cys 580 585 590Lys Glu Val Pro Asp Ala Cys Phe Asn His Asn Gly Glu His Arg Cys 595 600605 Glu Asn Thr Asp Pro Gly Tyr Asn Cys Leu Pro Cys Pro Pro Arg Phe 610615 620 Thr Gly Ser Gln Pro Phe Gly Gln Gly Val Glu His Ala Thr Ala Asn625 630 635 640 Lys Gln Val Cys Lys Pro Arg Asn Pro Cys Thr Asp Gly ThrHis Asp 645 650 655 Cys Asn Lys Asn Ala Lys Cys Asn Tyr Leu Gly His TyrSer Asp Pro 660 665 670 Met Tyr Arg Cys Glu Cys Lys Pro Gly Tyr Ala GlyAsn Gly Ile Ile 675 680 685 Cys Gly Glu Asp Thr Asp Leu Asp Gly Trp ProAsn Glu Asn Leu Val 690 695 700 Cys Val Ala Asn Ala Thr Tyr His Cys LysLys Asp Asn Cys Pro Asn 705 710 715 720 Leu Pro Asn Ser Gly Gln Glu AspTyr Asp Lys Asp Gly Ile Gly Asp 725 730 735 Ala Cys Asp Asp Asp Asp AspAsn Asp Lys Ile Pro Asp Asp Arg Asp 740 745 750 Asn Cys Pro Phe His TyrAsn Pro Ala Gln Tyr Asp Tyr Asp Arg Asp 755 760 765 Asp Val Gly Asp ArgCys Asp Asn Cys Pro Tyr Asn His Asn Pro Asp 770 775 780 Gln Ala Asp ThrAsp Asn Asn Gly Glu Gly Asp Ala Cys Ala Ala Asp 785 790 795 800 Ile AspGly Asp Gly Ile Leu Asn Glu Arg Asp Asn Cys Gln Tyr Val 805 810 815 TyrAsn Val Asp Gln Arg Asp Thr Asp Met Asp Gly Val Gly Asp Gln 820 825 830Cys Asp Asn Cys Pro Leu Glu His Asn Pro Asp Gln Leu Asp Ser Asp 835 840845 Ser Asp Arg Ile Gly Asp Thr Cys Asp Asn Asn Gln Asp Ile Asp Glu 850855 860 Asp Gly His Gln Asn Asn Leu Asp Asn Cys Pro Tyr Val Pro Asn Ala865 870 875 880 Asn Gln Ala Asp His Asp Lys Asp Gly Lys Gly Asp Ala CysAsp His 885 890 895 Asp Asp Asp Asn Asp Gly Ile Pro Asp Asp Lys Asp AsnCys Arg Leu 900 905 910 Val Pro Asn Pro Asp Gln Lys Asp Ser Asp Gly AspGly Arg Gly Asp 915 920 925 Ala Cys Lys Asp Asp Phe Asp His Asp Ser ValPro Asp Ile Asp Asp 930 935 940 Ile Cys Pro Glu Asn Val Asp Ile Ser GluThr Asp Phe Arg Arg Phe 945 950 955 960 Gln Met Ile Pro Leu Asp Pro LysGly Thr Ser Gln Asn Asp Pro Asn 965 970 975 Trp Val Val Arg His Gln GlyLys Glu Leu Val Gln Thr Val Asn Cys 980 985 990 Asp Pro Gly Leu Ala ValGly Tyr Asp Glu Phe Asn Ala Val Asp Phe 995 1000 1005 Ser Gly Thr PhePhe Ile Asn Thr Glu Arg Asp Asp Asp Tyr Ala Gly 1010 1015 1020 Phe ValPhe Gly Tyr Gln Ser Ser Ser Arg Phe Tyr Val Val Met Trp 1025 1030 10351040 Lys Gln Val Thr Gln Ser Tyr Trp Asp Thr Asn Pro Thr Arg Ala Gln1045 1050 1055 Gly Tyr Ser Gly Leu Ser Val Lys Val Val Asn Ser Thr ThrGly Pro 1060 1065 1070 Gly Glu His Leu Arg Asn Ala Leu Trp His Thr GlyAsn Thr Pro Gly 1075 1080 1085 Gln Val Arg Thr Leu Trp His Asp Pro ArgHis Ile Gly Trp Lys Asp 1090 1095 1100 Phe Thr Ala Tyr Arg Trp Arg LeuSer His Arg Pro Lys Thr Gly Phe 1105 1110 1115 1120 Ile Arg Val Val MetTyr Glu Gly Lys Lys Ile Met Ala Asp Ser Gly 1125 1130 1135 Pro Ile TyrAsp Lys Thr Tyr Ala Gly Gly Arg Leu Gly Leu Phe Val 1140 1145 1150 PheSer Gln Glu Met Val Phe Phe Ser Asp Leu Lys Tyr Glu Cys Arg 1155 11601165 Asp Pro 1170 3 175561 DNA Homo sapiens 3 aagcttctct aaactgaccttggaatttga accaccattt ataccatcat ttctgcatgg 60 aagtgtaatg taatttccaagaaaatgact tggagatgaa ttgtgagctc agtctgatca 120 gaagttggag gcagcctagattcctttggc ctcctggtcc ctttggcctc aacgtgcatt 180 gttatcctca ttcctaatccccagatgcaa atattgattg atgtttaaat ggcaactttt 240 cattaatgag cttagagcactttgtgaaaa tgtgtgctct tgccacctct gggacatctg 300 cctaagttgt ggtaagggttctggctagga gttaagagcc ctggttctag ttctgaagct 360 gccatacatt agcttaaagcctttgtgcaa gagagaggag aagagctata cctttgaggt 420 ccttgcattg ccattgtctatgatttatga ttttatggtg agtagatcac agacagtgac 480 aggatctttc catagctcccttttctggtc aaaatgcagc aagaatagtc caaccacttt 540 actaggatgt ctttatttttatggcttttc caccaaatgt attgctttcc ttagctggcc 600 tgggttttgg ggacagatatttggctatta tgtccacaaa tgcccttgac aacaggcaac 660 ggaggaggta ggagccagaagctttgtaag tttcataact tacagttcct gctgggattt 720 tgatttgatg aacctgtgtcaccttaataa attatgaagg cttctctttt ccttgaatct 780 ttccttgtgt ctttgttccaaaacaggcca aagaatgtgc tctagaaatt ggccatctcc 840 ctgttttgaa ggtgaaagattgctgggtgt cacccccaaa aggagacctc tctattatat 900 gtttctgtgg ttccaagctcactgtgagct gtgcctttga cttggcaaag gaattcatcg 960 tttgaaaagc tatttctagcccagcacagc tgttcactgc ttaagttcag caaatgccac 1020 catttttgca gactgtcctaatacccccag agaaattatg gcaacctaag tgtgtgactt 1080 tggtgagtga acaaagaatgaccacttgtc caaggtctcg cagaagtcat ccattgtaca 1140 tttgattata ttctctcctctcttatctgg tgcaatgtag gctcttaaaa gcaaaactca 1200 aaagaggttt tttgcactacgttccttccc tgatctttgt gaaatcaagg aatcagatga 1260 taatagtgat aaactaataacagcaacagc aattctgatt tctttgatgc ttcttatgtg 1320 gcaggtaata tactagatactttataaaaa ttatcatgct catttacaga tgagagaaaa 1380 gaggcatagc aagatcgtgcaatttgccaa agaaacttct taactaggaa atgtcagatg 1440 tggaatttaa acctgggttcaccttggtag aaggctcatc gtattccact atggcatgtg 1500 gattaaaata cggatgtggagatatggatg tggatgggga gctctgtaac tgccctggct 1560 ccgtttagct tacagatctttgttaaggtt ctttagcagt ttgcccctaa ttctgctaaa 1620 ataagatccg cctctctagcccgttcccca cctacacaat gacagaactt cttccctcag 1680 cctttcttct gtatctctgggaatacccca gtggcagtgg caggaggttg cagcacacat 1740 aactcatgcc actgcttgcccttccctagc ccgctctgct ggcctcctga tcacctagat 1800 gatgctgtct ggagctcccgctccagagcg cctgtcaatc tgataaagct ctgggatggc 1860 gctacactgc ctgtgacctctagttgacct tgggccatct ctcctccctt ctccagctac 1920 ttacaagccc ttgatgtccctgctcatccc aggtctctct ctggctggag gggaaagaac 1980 tcaaccttct gtaccctgcccttttgtgtg tttcattttg tgtgtggatg gtggaggtgg 2040 gaaggtgcag gccggcagcggccttccttg ggcccactct ggggttacag tttgttctgt 2100 ttctccctat actcctcccatccagtgcat gtaagccccg ggggtgctgg atcagaatgg 2160 ttcacatggt ggagtgctgtgctcagatgt gtggctcttt ttttggtcct gctactgaat 2220 gactctccag ggctccttctcctttctcag ttaaagggaa gtttctctcc cagggctcca 2280 tggtggacat gtctgtatatgaaaacccac agactccagg aaccacagaa tcaggaagtg 2340 tggctcaagg gagcaaggtggcatttcctc acagacactg ggtgtccaaa ttccaggggt 2400 agtcagggga ggtgtatgtgtgtggagtgc tggctggata atatttcgag ctagctagct 2460 attgtgattt tatccattttatccctgctt tttctcttgc cctcctgcac cggcccctac 2520 ccgccacctc ccattcaagcagataatagc tgactctgtg ctcaggcctc acaggaggtt 2580 tgcagcaagg cacaaagcaaagactttgct tctaagcttt ggaaacaaaa gcctaagccc 2640 tcagcagaag aggattcgatggaaaacaaa aacaaaaaca aaaaacaaaa gacagattgt 2700 cctgaagaaa catggaaggaaagaagcaat ttccccaaac tagaaagaaa tcccatttgg 2760 ttaacaggag gtgaatgcagaggtgacaga gagacagatg gggctccagg agcacagaac 2820 caagcactgc ttcctgggagagtgatgtca gcatgactca aaggcggcct cagcagagat 2880 gcttggggac ccgggagccacagcacagag tgactagacc tgaggggtca ggacagccag 2940 gcaagggcaa ggctgtctcagggatctgta gacagagaag cgaggaccag gtgggcctca 3000 ccccagaggc cttggcactgcgtgagctcc agaaactctg gcgttaataa ccacgggcga 3060 gggactcctg ggaaaactgaggttatgcat ttttttgttt attttttatt ttttctgcca 3120 gccagaagaa ataagagcttggcattataa attaagttgt tacagaaaac aaaagaaaac 3180 tcgtcaacct tgtgtttgtggactgggatt cacgccctct gccacctgcc ctcgtacccc 3240 tcgtgttcac ccagaaggagataagagagc ctgtgctcgt actctatcct gggcatttgg 3300 ctagacgcag gccttctgtcctggacccag gaataggtag cattgaccac gaatgcttcg 3360 tattacactt tcttttccatttcaataatg attcaacaaa acagttttaa atgaaaagca 3420 ttcggtaaac attgttcagtttttccagga ctttaagctt cagagggatg ccacaaaatc 3480 atcttcagag aagtttatggtgcttgtaag gctgagaaaa caagagtcct taagaaaagc 3540 tctgattaaa cattgtagagcattgtattt cctgccttgt aattgtgata gatggtatgg 3600 ttttgttttt aaacaacacctcatctttga aatgactctg ctggaaacct agcatttttt 3660 ttttttttca gaatatttccactgtgtata ttaaattgac taaacattca gaagaaggag 3720 cacaaatcag acccaggagagtctgttgca aagtgtaagt aggagagagg agatgttacc 3780 aatactgaaa atacactaaaattctgatga aagtaccaga ttcaatccca tgcaagcctc 3840 ttaggaacac tcaaactgttgaaaataaaa tggaaaaaag cgattacaca ctggcaccag 3900 catctaattg tttaatgaacattacatggc ctggatccta ttactaatgt ttctcttctt 3960 tgacatatgt ccactgatgaaggaaagttc tttttgggtt gcaaagtggc tttcagaatt 4020 tggacgctct gaaagataacaaacaggcaa catcttttac ttcttgctca caagtaaatt 4080 tctggcagtt tttgacaggtcataaacaca catgaaatgc taaacgtagt gggttgttag 4140 atgctttcct taaagttgaaaaacattttt aaaataagtt tccttatgtc tacttttttc 4200 cacattctca actctgacattcaaatgcac ttcacgaacc gacacgtaat aacatttgaa 4260 aaccaaattt ggaaaaccttcacgaattca gacttcactc ttagagaatt ttcaaagcat 4320 tcttgagaca agcaaatcgaggctgtgaat aagattactg agaataatgc ttacttaaga 4380 gaaatattca tgtttgttagatatgcacaa gcagttaatt acatttggag ttgtgcttgt 4440 ttaggtcctt ttgtgagagcactgacactg tttagaactt tctagaaaag ctttcatcca 4500 ttggtttctg tgacttcactcttccttgta tttccaatgg gctccctgga cgcatctcct 4560 caagctcttt tgtgggtttttcttcctcac ccagctactt caaaggtgga gttacttagg 4620 gttccatcca taaacctcttcttttctcac tccacaaact cacccaaagg tttgaattaa 4680 ctatatgcag ataatgctcatgtttatgtc tccagcctca atctcttcct tgagctccct 4740 catgtgtatc ctgtcttagtgggtgactcc actatctatt gctagtctaa gccttaaacc 4800 tggaatttat gcttaattcctctctccctt cccatcatca tcctaaatat cactttaaac 4860 atccaccctg tctcttcccactgttgtcaa cttattcctg ccatcatcct ttcttgtcca 4920 aatttctata gcaatcttttaactgatcca tctgcttcta aaacctttct cctgctaaga 4980 ccacagaaat ttttctagaatgcaaatctg aacgtgtcac tcctctgcta aaaaaacttg 5040 ttcagggccg ggcatgatggctcatgcctg taatctcagc actttggaaa gctgagacgg 5100 gtggatcacc tgaggtcaggagttcgagac cagcctggcc aacatggtga aaccccgtct 5160 ctactaaata tacaaaaaaaaaaaaaaaaa aaattagccg ggcatgctgg tgggtgcctg 5220 taatcccagc cacttgggaggctaagcagg agaattgctt gaacccagga ggtggaggtt 5280 gcagtgagcc gagatcgcgccattgcactc caacctaagc gacagagtga gactctgtct 5340 caacaacaac aacaacaacaaattgttcag gactctcatt gccctcaaga taaagcccac 5400 acttcgctta ggctgacaagggccttcatg atggagacca cattccaccc tcagcataac 5460 tcttgcacac cagactgaatgcactgtttg cattttctgt tccatctgat tagaatgcca 5520 tccccctcac ctccactctcccctggatct ataaagcttt cttccatcta agatatcacc 5580 tcccctggga agcttctcatccttcccaag attgaataag ggacctctcc tatatgcttc 5640 tatagtactt acatcctatattacctatat cacagcactc aacactctga atttcagcag 5700 cagctttatt catcagttttctcagatgat tgtaaaaacc ttcaaggtat cttcctcatc 5760 atatctccaa taatcagctcattctagatt cttgaaaaac atttgacaga tagatgaatg 5820 cagttacttt aaagatctgtttctcagttt cctgattcta aattctgaac agggagaatc 5880 taatttgccc agcttgggtcagtttccatc cctggtccaa tgagctgagc taggggtgtg 5940 agagtatgga tggaatctcttagaaaggag tgtggggtta gaagcaatgg gtggcatgca 6000 taaccaaatt atacaatgttcttcattgaa acaataaaac tgcattgtag tttttaaaag 6060 tcatatgtta tatatttttcattaattcat gagcctcctt tcattagttc attataaaaa 6120 ggtatcactg taccttcattttaatacata aaataaaaat tatacagtca ctttttactt 6180 attattaggg actcattcatccaactgttc acaaatatta ctaagtacct actatgtgcg 6240 aagctctgtg ctagggactcggtagatagc agaaaataaa atcaaatccc tgctgtcatg 6300 gtgcttgctt taaaaaatgtaaaacacaga tgcagcattt taaattagaa aacttaatat 6360 gaaagaagac aataggatgttctgcggaaa ataactgggg ggatgtgatt cagttatgag 6420 agccaaggaa ggattcccaatcgggggaaa aaattacatc aaagctaaga tctggcttct 6480 ctgggaagaa tgcctgggattggagagttg cgggaggaca aggacggaac tggagatccc 6540 tgtggagtta ttatgatacttgcagcaaaa gactatgacg cctatgactg ttgatgaatt 6600 caggagattc tgggagccagaactgattgc actttgattg tttctatgta gggtgaatta 6660 aaggtgctaa gaataacagttggggtagag gttaagcaca tgaactctga agctagattg 6720 tctgagttta aatcccagttccattagtaa gttatgtgac cttgggcaaa ttacttaacc 6780 tcactgtgcc ttggtttcctcatctaaaaa gtgggtgata ataatagtag ctaccttgaa 6840 gatttgttat gaggattaaacaagttaaca tgcataaaac atttcaaaca ttgcctgcat 6900 gtcggtttac ttacaaactgaaaaagaggt aagtttccat gcttctggct tgagcaatgg 6960 agcgatacag gtgacatttattgagatggg aaagactagc agaaaaagag actttgatgg 7020 actgaagatc acagtttagccttagataca ggcttgagat tgctcagacc tcttggacat 7080 ctctctttgg gcaggagtctgatctcagaa gagatccctg ggctggagat gtcaatctgt 7140 aagacttcca aatacaggcaacatttgaag cccagggatt ggaaaggcaa gcaaggaaga 7200 gtgtgtagag tgagcaaagtgtgaatacag ggccaaagac tgaccatgag agttccgaaa 7260 gccaagtgga gaatgaggggccaactactg gtttatgtgc agtgctgtta tgcacatgca 7320 ttagacaagt ggaaatagaaacatctggta taatttgaat ataaggaata ttcaaacaaa 7380 ggacatcctt gtatttctattttaatggag cttaaaactc taattttctg tttagccctg 7440 ttacattgat tttatggcttctcctatccc tcattggaat ctctgatcca aagatttaaa 7500 agaaagtctg gagaatttggagatatgagt agtttcatgg ctggatcttc aatggcgaag 7560 acagaaggtg gggcataaactagaaccagg tgatgaaatg tcttataagc tgtcatagga 7620 gtttaaactt gataacagtaaggagccatt caaagtgtta agcaacagtg aagcacgatt 7680 atttcgtatt tgagaaagatctttgttgaa caaggtagaa aatgcattga aaaggagcaa 7740 atccagaagc tgagaggccaatggtgcatc tgtaacagta acccaggaaa gaaaaggcaa 7800 cacaaaggca aagaaaagacaaaccaggac tgtgggggag gccaacggtt ccaatttaag 7860 taatgaccac agcaagaaacagacaggatg aggtgattga ctagttagag aaggtaagag 7920 agaggatgac gccatggcctctgcttttgt tcagactatt ctgtgccctt aaaatattac 7980 atttttccta atccatccttatgctccatg cagcaccctc aatgtcttct gtagggataa 8040 ccctttaatt ttcaaatatgttctcatcct gtctcctgat gaatgcatgc agtcattaat 8100 tagctcaagt ggaaaataaagatgcgactt acttcatatg cactctaggt cccaggcctg 8160 ggtagaaaaa aaaatctattacctaaacca cagaagctaa aatcctccac aaagaagaaa 8220 ataccccagt gattgatttggtctccacat cacctggctt aactaagtat agaagtatta 8280 atatgctaga ccctatttattaaaataatt tagctcatca ttgtgacaga aagcccatgt 8340 ttcaaacagc aatatttttgtaataaatta gaagtccctc aacaaaccct gttaaacccc 8400 atgtggattt ccgttaatggggaagatttc ccagtgacct agcttaacac tgctgctgca 8460 cattaatttg ctttccagctatacataaat aaataagtgg ctgcctcctt gatgtgagct 8520 ggaggaccca ggggaaggcatttttacctc taggtatctt ccccagactt cttatgctgt 8580 ttataattat catattcatcatttttttct tctctctctt cccaatttct ttcttcctag 8640 tctctcttga acctattcctatcagactta tgcccccacc attccatcaa aactgcttgt 8700 caaggtcacc aatgacctccattttgctaa atccaatggt caggcatcag tcctcattta 8760 acatgatcca tcaattgcattcagtgcagc aaatcaccac ctcttccttg acacacattc 8820 ttcatttggc atcaagaataacacattatt tccatttttt tcctacctca ttgggcttag 8880 ccttccttgc tggtgttctccatggtctct tagtgttcag aatacctcag ggctcagttc 8940 ctagtcctct tctctataacttaaaccctt gatcgtctca tcccgtctca tggtattaaa 9000 taacaccttt accctaacaactcttcagtt agtacctcct ggtctccagc cttactcagc 9060 atctccactt ggatgttcaatagacatctc aaactcaatg tgtcaaatat ggaactcctc 9120 atctcccttc aaaaactgttctactcatag ccttccttat ctcagttgat agcaacttga 9180 ttaagccagt gctcatgccaaaaatcttga agtcatcttt ctctctttct ctcacaatcc 9240 acatctaatc ttgttgatagtgtctgcaag gtacacccag aatccattct cttcctacct 9300 ttccactgct accatcctgatccaccaact cttggctcaa agtcactaca atatactccc 9360 agcttctctt cctgcttatacacccttgtc tagcaccaga accatagctc aagtaatcct 9420 tcttaaaaag taatcagtggcatcacttct ttactcaaaa actgcaatga cacagggtaa 9480 aagccaaagc cttaaaaatgccttcaagct cctacctggt ctgctccctc ccctgagttt 9540 caacctcatc tcgtggtctcccctgctcac tctgctcctg accactggcc tccttgctgt 9600 ttctgagaca tgccagacaagctctatgtg tgccctctgc cttgaatgct gtcggatact 9660 agaggccagc cccacccttctttcatggct ttgttcaaat gccatcttcc caatgccact 9720 tatcctgcca tatttcatactgtcacttgc cccagcccca gtggtcccag cctccttgcc 9780 ctgctttact tttttctttttccatagcac atatcacttc ctattatgtg atgcaatctg 9840 cttatttatt aggttcactatcaactgtca tctctgctgg agtgtaagaa tcacaaaggc 9900 agaggtcttt gttttgttcactgatgtatc ctgaacacca agaacagtgc ctggaacata 9960 ggaggcactc agtagatgcttattgaatta atgttttagg cttcaatcac aaggggccct 10020 agtataaggc aaatattattggacaggtga tacattcagc ttcaggtttc caatctgagc 10080 cattaacata tcttacaagtttgaacgaac tacaaatgag atgtctttga tccatttcct 10140 ttctagctca ctccttcaacacatttttca tcccagtgct ctggtcctct cctcaggaca 10200 gggaatggat acagtcaatagtgtctgttc agggtttatc ctccctagag taatttggtc 10260 ctctttctcc ttattcccatgaaatctgaa ctgtgatcca atttctcaag accttaaaac 10320 taaaaaaaat gagaactagtgaccccattt taagaaatat tgcagattgt aaattaagcc 10380 aatgctgctg ttaaaccagatttagctcat tgacatctag aggaaataca aacgattgcc 10440 agaactttat agcaagagagaaggatacgg tatagtggga aacccaagca aagggctgga 10500 ttgggagctc tatgttactagaggctaaca tgaatgtccc agaggagcga aaaagaggga 10560 cacaaatgga aggaacagatcatggggcac acaccttatt tttgcctaag gtggacaact 10620 gggctaattc accaaaggttttgtgagccc cccatcgtgc tgctggcacc taggatagcc 10680 gggtggaggc ttctccaacaattctaagaa atacccatgg aatgagaaat gcccaggcag 10740 atatgtggca agcgctatctcccctggagc tcttctggag accctgggcc acttccacaa 10800 tgctttctac actgagaacactctacactc tcactctaca ctgagaacac tcattaggat 10860 caacactgag aacactcattaggatcaact agaggcaggt tgagttttta tttcactaag 10920 tacccaagct cttgtgcataattaatcctc aaaaaactaa tttctttccc agcactgaat 10980 tatccttggt caaaaagaaatcaacatgat aaataccagg cacatagtgg gcatttgaca 11040 tccttcttcc catgtctctcgttttcctct agtacccacc ctcccaacct cctttgagat 11100 gactattttc catatttgcttctttcatgg catatctgta ttgcttcgta tttggcccca 11160 gatgtcagat tggcttgcattactccaggc tgaatcattt gatttcctgt ccatgttcct 11220 aacctttcca gatcctcttgtatcatttct cagtcattcc cgctgtttac aatacctctc 11280 aatttaaagc catctgcaaatctcattaac aaaccactga ctccttctcc ctcacgatca 11340 taggacatgt tgaatgaaacaagacctgac agcaatcctc tcctctccat ttgaggcacc 11400 atttctctgg gttactttaacttattatca tttttatatt atattgttat tgcacatgta 11460 ctcccagcct aggcatatctaatatgtgaa aatcgggatt catcattttt tgaaattaag 11520 ttattcacag gctgggcgcggtggctcatg cctgtaatcc cagaactttg ggaggccgag 11580 gagggtgggt catttgaggccaggagttcc agaccagctt ggccaacatg gtgaaacccc 11640 atctttacta aaaatacaaaaagtagccag gtgtggtggc ccacgcctgt ggtcccagct 11700 actcaggagg ctgaggcacgagaatcgttt gaacccggct ggtggaggtt acagtgagct 11760 gagattttgc cgttgcactctagcctgggt gacagagcaa gaccctgtct caaaaaaaaa 11820 aaaaaaaaaa aagttattcaccctacaaaa acttaattat attaatccct ttactagtga 11880 cttttcctag tatacttaaaatactttgct tacaaagtaa taattaccca acttctcaag 11940 gctggttaca gccttgaatataggttttta atacccttta gctacatctg tctgtaagaa 12000 attttttcct agtagagtttttgaataaga ttcaaattct ttcacatcaa tattagtaag 12060 catctgagga acacaccagttttctcactg gcgattcatt ggatcttggc ttcaactttt 12120 ctcacccctt ttcctgatatgctcaaaata tttccaaatc tcactattct tccttcctta 12180 aaactttttg tgtcccttctatgtgccaag cactgtgctc agtgctgggg tacaataaag 12240 aaaagagtct ccattctctggagctgttaa tccaagaaaa aactaaaggg aagtagtact 12300 tttgagtacc tgccatattctggacctgca atgtatataa tcccttttac accccacaga 12360 aaccccatct aatagctgagaaaacaggag ttcagagaag ttaattaatt tgcctgtgat 12420 gcagaataca aatagacaatttacagaaga aatacaaatg gacgatagac atatggaaaa 12480 aatactcaac catgcagttattaagtaact ggtttattaa gtaaataaaa tgctactttt 12540 ttacctatca aggtggtaaagagaaaaata atactctacg ctggtggggt tgcagtaata 12600 caggctctct tgcatagggctgcagggaac ataaaggggc aaaatcctcc tgagaaactg 12660 tataatatgt aacaaggaccattataagag gaactcacct tagtgtccat cgcaagggaa 12720 tgaaataaat gatgttacatccatccaatg aaattgtaca tagccactaa aaatcatata 12780 gaatatgtaa tgacctagaaagatttacca aatattaaat gaaaaaggca tattacagaa 12840 tagtagatac tactggatgacaatatatac aggattgtgt gtatgtgggt gtggagaaaa 12900 taaaagactg gaagaatggataatatgtta acaggggtta ttcataaaag atacgacaga 12960 gttctttcta ttggtatcttctacatttca attatttaaa aaacccaaac ataaaaatgt 13020 tcatacattc agatctagttatgcaactgt attgcaataa acagcaatgc agactaagag 13080 tcaagtaact tgctcagaaccacccatcgg gtatattact ttaagtctcg ctttctaagt 13140 ctgaaatcca ttttctttctacaacctact cattcagctc cataacccca attatatcct 13200 aactagagta atcccttgatgacctttcta gtttctaggc tttttatcac gagattaatt 13260 tctcccacca aaatggtttgctgttctaaa atctctaagc tggcactctt tctgcgataa 13320 gcaactcttt cttttctcaaagcccgcttg ctggcaggtc atcccgattt tcatcatctg 13380 cttttgttct gtgaaatctgatgagggctt acgaggtctt cagcatctct caacagcaaa 13440 cattggtatt tgggtttgaacaattctttt tgttgagcag ggctttgtta gccctagcca 13500 gcttaaatgc cttcaaggcttcactgtgac aacagaactg ccaccacaca tttccaaaca 13560 catcccaagg gtggccaattgagaaccacc ctatgatcgg ccacagctaa ccctctgacc 13620 tcatctttct ctactccctggctttctggc tccattccag ccacactggg ctccttgctg 13680 cccttctcat aagcccacaaaagcagggat ttggtggtgg ggattggggt ggaggggtgt 13740 tcctttcatc tgtacaaccagttcctgtat tcaattccct cttttaagca cttgatttgt 13800 ttttgatccc ttttaaatttgattcagctt ccccaactgt aaatgaacta accaatgaaa 13860 ctaatcactg attccacatgttcccaagct gtgcacttct accgcaggat cttccaaggt 13920 ctgaactgaa aactgcgtctgcatttcctt ttaccttcta tgttttggga aggtatctct 13980 aaactatctc ttcaggacagaagagaaaaa ctacatggat gcaaatcagt catactgcat 14040 ttaaattatg tgcatctctctgttattgtt agttccttga gggcagtctt tgtaaccttc 14100 tctttatatt ctccccaaataaatgagtgc ctggaaatca taggtactca agacctccac 14160 agcagcctcc tccctgagagattaggtggc tggaatgtga ttagacacgt gggcatctcc 14220 aggcttccac acagcagtcattactgagtg acagatgtca atcctggcat gaatgtgatt 14280 tgcaaggctc caaaacggagattggattaa atttgcttaa atttccatta ggctgtgtgt 14340 gtgtgttatt ttttcctttctttttggtct tttttctctt aatttctaca tctgctatat 14400 acactcccca ccgttttctaaaccatttta gcagcagtca accctgtgaa ttggatgtca 14460 tatgcaatac aaaatgtaaatcatgttcat tagatgaaaa acgcaatttc ccccctcatg 14520 gagaaaactg ggacaacacatatcagaagg ggcacaatat gacagtcttc aaataagtca 14580 gtgtgcttcc tgcatttaaagctgaaatct gtttggtctt aattgtgata tttgcaaagt 14640 gtcatgcttt cttctacattgaaagatttc ttagctactt caaaatgtga attcgtgagc 14700 ggactacctt ctccaaactaggatctgggg aaccaatcct aattgtattt atacatgcta 14760 aagttcccct gttctttccactcacgcaaa acgcaggata aatataatag cttctcttcc 14820 acttggaaaa gcacaagttttggagccttg agttggctgc actggaaatt agaaatgtct 14880 ttgatgaggc acagctgtcttcatttctta gtcacaaatg tctaaagcat tccaggaggc 14940 tgactcatat acctggagagggaccatggt ttctcatttt ttctcctgaa gcccttgact 15000 ggtcaggaag agaccagtttctcctttgtt ctacagctag gaacttaact acaggatgat 15060 cttaagtaag ataaaacctgacatttcttc accaccattt gcttttcatt ctcttacatt 15120 tgtctcaaac ataatgtgcagcacttagtg atctttctgc tatggagact cactgaagac 15180 aagatcccaa catggcaaaacttaagagtt ttctcaccaa tagctgctat ctgcagcaaa 15240 ggaaagagag caccttgtgttgtagcctac cgtccactga aggagatcag tatggacatc 15300 caggagtttc tgagttccccagatagtctc agacgaaagt caaacctctg agatgagcaa 15360 ttaagaacca acatttttgaaactctttgt catatggttc gcagctttgt gactctaaat 15420 agttgtcttt ccgcaaataattccattatt ccacaacagc aactcctctt ttctgttctc 15480 ttgcagcaag caagtgatcattactcacca cctcaatcca cccaagggct ttcacctgga 15540 acccaaagtg cgtaagtaattaagatttgt gttctttggg gaaaggagta tcgacaaaga 15600 tgtagcttgc ttctcatgtgagaaaagtac ttacatacct acacagttct acttggaagg 15660 cctatgcatt ttactatatgtaaattacaa ctcaatttta aaaaaatcta ctagcaaaga 15720 cacccattct ctcagggaacttgcccttat ttaacctaca gctataaatg acagggttct 15780 ctagaaccat atactttgtcccataaacga agcagtaaaa aaacctgaat atcttccctc 15840 caactaaaat tacattatttttctcagtta aatcttccat tcaattacaa tggactatca 15900 ctatactatc tgggtgagcaaaaggtataa tttacaagaa acttacagag ctgaagggat 15960 gagagaacta taaccctgcaaagttgtgtg agcatctgag gtcctacaca aaccagtggc 16020 ctagagaaca ggcctctaacctcccttagc ctggagtggc cagagcacaa gtggtctcaa 16080 cacataacag aaagaatggacattttctgg ccaggcacgg tggctcacgc ctgtaatccc 16140 agcactctgg gagaccgagacgggtggatc acctgaggtc aggagttcaa gaccagcctg 16200 gccaacatgg tgaaaccccgtctctactta aaaaaaaaaa aaaaaatacc aaaaaaaaaa 16260 aaaaaaaatt tagccgggtaaggtggcagg tgcctgtaat cccagctact tgggaggcta 16320 aggcaggaga attgcttgaacccaggtggc gaaggttgca gtgagccgag ttcacaccat 16380 tgcactccag cctgggtgataagagcaaaa ctctgtctca aaaaaaaaaa aaaagaatgg 16440 acatttttta aaaacccactatgtctatag ctcagtccca gccctgaaca accacattca 16500 accagcaaaa aattcttcgcgatgaaaaga tttacctcaa agaataaata acattaaatc 16560 gagaattcat aagcagtcaggagatttgag atttttattg agaaaatagc tattttgaca 16620 ttatatccag tttctcatttatcagaataa attctttaca atattgatca ccatcaccac 16680 tttagtgtat ttgtacatagtctctgagta aaatatattc acactcggca aggctagaat 16740 attgaaatta tggccaacattgcttacttt aagattgttt actttataaa gaagctagag 16800 tagttgtgca actagaacagatgtttttaa aatgtttgcc attcaaagat aggcttggtg 16860 ggacaaaact aatatgcatactacatacat atatttcttg tcttctttac tgtcaatctt 16920 tcagaacagt aacatgacattacaaacacc tcaaattccc acttcaaaat gaacagaaaa 16980 atggaaaaac attatttcccatttcataaa attaaaaatc aagtcagaag agaagtaaaa 17040 ctcattttta tgcatttaacttaaaagctt gaatacacga ctcctcctag agagaaggaa 17100 gccagaactt cagaagtagccagtggtcca aagaataaat ggccccatga ccttctctat 17160 ggttcatgac ttactgagggctgatgcaaa ctctggcaag ttatttttca tgatttccaa 17220 ggatctggga tatgtaaacgaaatgattaa aagacatttc tctgaatttg caagaagacg 17280 actgaagaat cagaacaaagatccaacggc ctttcacgtg gctacatgtt caccattaca 17340 ccacaactca aaacccacaggcgagctttc tctcaaatac acattcaaaa tggtgttcca 17400 aataatccaa ttgtatacattgcaaagaca cacaaaatgc ctccttacac acgattagta 17460 atctggtttt agactctgaagtgataccaa acatatttag ctaaccctct caaaattgca 17520 ggcctagtga ggtttaaatgttttaggata tagaatctgc ttagaattta gtgaaaacta 17580 tgaaccgtag actgtcttcggtaatgaaca aaactggaaa atattaaact tcctttgctt 17640 tgtcactgag caactataagctttctatga tgtatgtatc atactagctt gtatgtgact 17700 ttagtgtcat aaacacccattgcctttttg gcacttttaa agtacagcca gaaaaggttt 17760 ggaaaattgt tctggaaatacaagttggtt ccctctgtct catggatgct taggaactgg 17820 gtagtctgct gaaaccaccagttcagcaga cccttgtaca gctgttcccg gccaactggg 17880 atcctaagga aacaggctggaagagccagg ggcctccctc acaaatgcct aaataaccaa 17940 catatctatg ccgacagccatccctctctc aatgccatgg tccaagggag tcttgacctg 18000 tctcttccca ttttactaccactggctaga ggggcaccca acaacaagaa acctttgtaa 18060 tgagtatgaa ctagcaaaaacactgtaggg cagcatttcc ttaggtgtat ctctcagaac 18120 ataaatccca caagatacctacttttaaaa aaatctggcc ggggacagtg gctcatacct 18180 ataattccag cactgtgggagggtgaggcg ggcagaatca cctgaggtca ggagttcaaa 18240 atcagcctgg tcaacatggtgaaaccccat ctctactaaa aatacaaaaa ttagctgggc 18300 gtggtggcag gtgcctgtaatcccagtaaa ttgggaggct gaggcaggag aatcgcttga 18360 acctgggagg cggaggttgtagtgagccga gatcgtgcca ctgcactcca gcctgggtga 18420 caagaccaag actccgtctcaagaaaacaa aaacaagaaa tccatattaa aataaattag 18480 ggaaatgatg tataaaatagaggtgcttct tggagattta aaatgcacat tagcgtatca 18540 aaggtctgaa aggccaacagtaactcaact ttgtctaacc cactatttcc caaacttact 18600 tggccacaga accctctattctcctaacaa tatattctga caaaacactc cttcaggaac 18660 tgcttcaccc agaaccaaacaggtgagttt taaaaaatat tatagcataa tttcaaatga 18720 ttaataagga tacaaagagacttattttcc tgtcttttgt tattttgtct acttattttg 18780 ttctgaataa tcttctatcacatttcccct tctattcaca aaccttcaac tcgagttacc 18840 ctttctgata cctttgttcaagaatgttaa ttcttatcag aaatccatgg cacactagtc 18900 tggaaaaaaa taattttgtgtctttttaaa ttcctagagt acctctccac ttcaatcaca 18960 atttaagtcc agggcaggtatcctgggctc ctagcaaaca ctacacccca acaagagttt 19020 gggagccaaa ttattttgcaaacccttaag aacacaaact aaaactaatt tatccttata 19080 tctctgcaaa aattagcattttgaacatcc aacgaaactt caagtttgag gataatgggc 19140 ttactgacct gacctttgatttcacgtaac taacaacaaa tttctttaaa atctggttaa 19200 gaaatatgaa aatattgaaaagtaaacata aattccacaa agctgaatgg cagcaaaaaa 19260 tatttcttaa catcaagaacatatacaaaa gatgatcatt accacccaaa ttatctctgt 19320 tgctcaggat ttcagttataaaaatagcca aatcaagaca aagacaatta cagcttaaaa 19380 cgaaaagcta caccttcttcattaaaggtg agcatcttgt cacactgtgc atatggagag 19440 agaacaagta caaggcacgggagaactacc atgtcaaggg acagagcttt ttcaaaatat 19500 tttatcctca gaagtaaaccccactaccat agaaagaaaa aaatataatt agatatttta 19560 aatcacagca tttttgacttcctagggaaa aggagttttt ggttttaggg tttttttttt 19620 cttttctggt aaggcaaaatagatctttag atatcatttg ttacaaacat gagtttttct 19680 taagaatgaa ataaaaattctatttaactt ctatcatata tttattcaac taagttgaat 19740 actatgttta gaaaattaaagttttaaatc atggtttata tatagataat gatacattta 19800 atctatgaat ttactttaaaaccactttta ttttgaaata cttaagactc acaagaagtt 19860 ataaaaatag ttcagagagatcccaggtac ccactgctta gcttcctttc tttacatgta 19920 agcatttata tctaggttttaaaagggttg aagtttttga cagttgatga aaaactgtca 19980 aaaaattgta acaagcctggtaaatgcata tttttggagg aggttctaag gaaacactac 20040 caagttcaag taccacttttatcacatgca gctgccttaa ccaacaggtt ttctaagata 20100 ctatccccct tacctgtttctgcctctttc aatggtgttt ttccattttt acagacttct 20160 gaaaatttta gctttgattgaaataagctt ccccattcct tcatgttaat atatctagca 20220 atattgaata gaaattataaatggaaataa aaatgcttgc ttttataaaa tctccagtct 20280 cgcagcaccc ccaatataatacaaacaaac ttaagttgaa atttggtttg ttaatgccca 20340 ccttgtgtgg tcaaaacacagttttgaagg aatgaccacc ttcaatgttc tttacagctt 20400 ctttagtgtt acttaaaaaaaaaaaaaaaa tcaatctgat ggatgattga tggtagtttg 20460 ttcatggaag atcttcatcttatgggaatt atctagtttt tctaatcata tactaccaac 20520 aaaaataaac acaagcatgttccctttaat catattatcc tccaccatta cttccaaaag 20580 gcattggctc acagtattctgtttaagatt gtggaccatt tatcccaatg aactcactca 20640 aactttatct aaaacatatcatgacaggct ggtccaacag tctgttcagt ctgaaaaaat 20700 gttctgtgct ctgttcagattgacaaaatg ttctttttgg ccgggcgcag tggctcacgc 20760 ctgtaatccc agcactttgggaggccgagg caggtgaatc acttgaggtc aggagttcaa 20820 gaccagcctg agcaacatggtgaaaccccg tctcgactaa aaaaaaaaca aaattagctg 20880 ggcatggtgg cacatgcctgtaatcccagc tacttgggag gctgagacag gagacttgct 20940 tgaacccagg aggtagaggttgcagtgagc cgagactgcg ctactgcact ccagcctggg 21000 caacaagagt gaaactctgtctcaaaaaaa aaaaaaagtt cttttctaag agcttcctgt 21060 atgtgtggct gctccctaaagtactttgtg ctcagttaca gcttcctgtc atctcattta 21120 cttatacata aatagctcaactaagtaacc caacatataa actctcacta aactcacatg 21180 ttgcatctta gaaaaggtgatctggaagca ttatggacta tagtgagaat gtaaggctaa 21240 cagcagttca aagtagggatgacacaaaaa caaataaatc atattgacag tcattccctc 21300 tactaaaaaa gaataatacttgttattcta aaatgacctc ttttgattaa aatttcagtt 21360 cataaaacat tacagtatgcttctttatca aagtctacat atcagaagaa catgagctcc 21420 acgttcctgt aaaactttaaacacattttt cttcctaata gacattagca gttttgttga 21480 aaagaaaaat tgacataacctagcattatc ttttgacttt gcccatggag aaacttggag 21540 ttaaatttac tacttaattttagaatctgt cagtgttgat atggttctat aaatttttaa 21600 tttttttaat ttaaatttttaaaattttta attctttaaa atttaaaatt tttaaattgt 21660 cctgttttac atgtaatatacttgtaatat acatgtaatg tacttgtacg ttataactgt 21720 aatatatttc aaatactttttggagggtct gatataaatc tgatataaat taaaaatgac 21780 tggccttgac cacagcataagcagatgtct gatgaatgtg ctaaccaccc ttctccaata 21840 tacatccatc ctagaggacaataaagtctc acagtcatga tttccaatat aacatattta 21900 ggtttcagac tgctggaataatagatttca gattgctgga atctaataat ctgcctttga 21960 agatgactga cacacacaaacacacacaca cacaccctgt tgtagaagta atgtgcaact 22020 catgaggtac tttccctcatccaaacagat gaccaagatt ttgaagacta ccttatttct 22080 cacttagtta aaactttcaagaaaaaatat ttagaatgac ttaaagactc ttgtataaat 22140 aactacctaa atacagaacaaattgggagg atgtgggtca aacacagctg agctccactc 22200 agctcaaatt agatgagtcttttgactaca aagctcattt tcttaacata tgggttcctg 22260 cacgacttga taagatttgctgagcaccac agtaatcttt taagacacta atcaaaaaca 22320 tggttctaca ttttaaaaacctcagaagta gttttaggac aacagaagca cctcaaccac 22380 taaaataagc aagtttctattataagtaaa attaccacac agcaaatccc ttaacagttc 22440 agcaaccaat aaatagaaaatattcattaa aagctgctat ttctgattgt tcctttgtat 22500 tcttcgacta taagtgaaacatatgaaaaa taattggttg tcagaaagct tttgactaca 22560 tgatatgcat cttatgaaagaatatgcatc ttatgaaaga atatgagcca agcatagaaa 22620 acttggacca gatgcacagtatttctacta ccctggccat gggctcctcc tgagtagtgt 22680 ctaacaagtg ggcaatttcagtaagaattt tacagcctag catagtaacg ataaccagta 22740 cattcacttc ttctggaattaatacaagaa agacaactga agatgtacta caaagacata 22800 ggtctaaaac tgccacatttgcccaagtaa actaaaagaa ctcttcctaa cctatatcga 22860 acacatcagc aaagaccaatgttatactga aatgagtatt atcagatgtt attatatgta 22920 accaccttta atatttatctatgtgctact aaacccccaa aagattagac ttatgagagc 22980 ataattaaaa agcacattcctcctcacttt ttaaagttaa atatggtctc ttctactgaa 23040 gcaatttatc tttttctggttcctaaaata ggcatcaaat ttctaaacag aagtaaaaag 23100 cctcagccaa aaagccacatattaggtatt cagcctggtc taagaaaact aagttaataa 23160 attgaataaa ttaaatcaaataaattagga gtgcacattc agaacagaat tgtagccttt 23220 ggtcagcaac atgtgaaccaaaggcaaaaa tctgccatga ttaaggcagt tctttgtata 23280 cagtacgatg tgtttttcttccccgccccc ccaaccaagt tcatttagca ttctctttgt 23340 tattaaaaat gcagtgccaagctagaatac aagcttccta ataataatag taaaaataga 23400 tggcatagaa agttccttttggtttagagt cttccttaat acctattatg gtatcttctt 23460 tttgccttca cacacttggtgtaagagatt actgcaacaa tcgtttgact ttggccacaa 23520 accatgacta ctctcagggcgaaagccgtt ggaaggaatt attttcttct tctgctggag 23580 tcaatttaag tgttggcagtttccgaggag gaagctgagg gctttggcga agattgtcat 23640 ccatctgtag aaagtacaaaagatacatct gactaatgct gtggcaccgc ttatgtggac 23700 tgtggcctcc ccatccaaactatagcctag aagagtacag aaccatatgt ttcctgggct 23760 gctagagaaa cctttttcttacaaataatc tcaagaattc taaatgtcat acctcaaata 23820 ccagctgact cagtttctgtaaaaagcaac gtggctcatt cctgatacct tcacagaatt 23880 atagttaatt ccacaaaataaatattctac tgaactcaac cataacttaa agaggtcttc 23940 tgatagtaag tgtaaaagcggtactgttac actaagctcc agtttgcaaa ctatgttcac 24000 gtacagtatc ttatttgatcttcccgatac gctatgaaga aaaggcagac acagaccttc 24060 tctgttgtac agatgagcaaaacgagactc tgtaaggtta agtgatttgc cccacgtcac 24120 aagctagtat gagagctgaaacttgaaccc aggtcttctg attccaaaac tattattctt 24180 tcaactatac ttctttacctcaatgaaaat gacctgtatt ccaatcaaaa aagaccagct 24240 ttttaaaact gagaataggtagaccaaagg aagctagtac attaggcaaa aagaaagata 24300 aattctttaa atgtaatccagtggttttcc aaacactgga tcacttctaa atgaggtaac 24360 ccacatcaag ctcaaataacacagtggtgg acctcaaaaa ctgttagcca gtgtcatcag 24420 gcctcattgc cctatgagtttgtaaaatag taagtattac tatttgtatc aggaaaaaaa 24480 aaaaaagcaa gcttatcagttacaggggaa aaaatatgta acctctttgt atttttccta 24540 gtagaatcac acttgaaatttcctttgttg gttctctccc ctacccagtt cctcaatgtt 24600 tctctcatga aaagaacaccccaagtggca aaatatctac actggtgaaa aaactataaa 24660 ctatatctag agcagagaagaacaagaagg agaaaaaaat aaaactttca tgtggaaaga 24720 agaaaagaac acggtgctgttaataagaaa ggataagccc agtgcttaca atcttaagaa 24780 acattaagag acagagaggcagacacacaa gcaagaagtg tttttgcttt gttttttttt 24840 gtgttttttc tgatggagtctcgctccgtt gcccaggctg gagtgcagtg gcacgatctc 24900 agctcactgc aagctccgcctcccgggttc atgccattct cctgcctcag cctcctgagt 24960 agctgggact acaggcgcccgccaccatgc ccggctaatt ttttgtattt ttagtagaga 25020 cggggtttcg ccgtataagccaggatggtc tcgatctcat gacctcatga tctgcccgcc 25080 tcggcctccc aaagagctgggattacagga gtgacccacc gcgcccggcc gctttgtttt 25140 tttaaagcaa atcttttggatttcctagct taaagggtac taactgtgtg gcaaaagtaa 25200 acccaatact cttatctgatctttccccaa tcaggccact gttggcttca aaacactatg 25260 ttacatacaa ctttagtgaaagtatattta aacagttttc tgaaaaacaa actctataaa 25320 aaaaactaaa agggtacagttaatgtgtga cccaaatgac aatattagct accgaaagga 25380 aaatactgaa ctatgataaaatgattgaag tagacttgac cttttcaatc aagttggatt 25440 ccttatttac aagttgtgtgagtttctgca gatttgcatc taccgtttct tgtccagctg 25500 gagagatgcc taagaagccaggacagaaat cagagaaaga caggaaattt ttaaagcctt 25560 taaagctatt tagattgaaagaattttgag tctacccacc ctgtccagtg ccccaccctc 25620 cccaaaaaag gcaaagaggaaaaccattct gggtttaaag gcgaagagag ggattcgaat 25680 gctctaaaac taaaccctgagcaaaacgga caaaaattta tccactaagt agacactgct 25740 aaccaagtgt tctgcaattggccacaataa ttagtttttt tttttttttg aaaaaaaaag 25800 cacagtagtc ctcatttccttctcaagttt aagctattcc tttctcacac ccatttcaaa 25860 taggtctttg gtcacaactatctggatagt tttctaaggc agtaactccc aaaaagttgc 25920 ccaccagaca tccaaagtattcagaagaac ttaaaaatac aaattcctag gattgatgcc 25980 taaaagattc taatttagtaagtctggaat aggacccaag attttttatc tgtcaaataa 26040 tcaggtttgc taaccactgctcccaagtta acacggttga acattactta atttcatgac 26100 tcccaaacta ctaaccccttcctgtataca gaatacataa cccaccaaaa aataataaac 26160 tgtccaccta agccagctatctttataacc tgtttcttaa aaggacaatt tcagccaacc 26220 atcccaatag aaatttagtatctgaatcac ttaagcacaa ggctccactc tctcaatgaa 26280 gcacatccta agtaacaggtgccctctatt tactatactt ccattctgat gcaatcagtt 26340 ttcaagtagt ggaatcatggcagtgtacct caccaaaatc aatctgctgc tgatttctgt 26400 tcatgcagga aaatgctagaagcagcaggt aaaaatcatt taaagtaatt gtccatttgc 26460 attaggataa atactgtttttcagcaaaga cttaatacct gtggttttaa ccttttagaa 26520 gaaaatgacc cacttcaaaggggtaatatt taaagaacaa gaagaagaaa cgtaaaaatc 26580 cagcggaata gataaaaggcaaatatctat ttatctattt aaataagaac taacatgaga 26640 ataaaaatga agtcggaaaaacacaaatcc ttgaagtgct ataaaccact atttaaagac 26700 aatacacaaa cttctaaaattaaaataagt agtggatttt gcttatacat acataggaga 26760 atatgcaaac aaacattagctgaaaaagag ccagcttacc tccaagacta agcctaaaat 26820 aactgctcag gatgtcatcacaaaagcgac agaggttgga gagctgtttc agatgttctt 26880 gtagctgaac tttaggaaaccgtactttgt aaagaggtgc aagaaaacca aaaacatagg 26940 catccaaggt agaaggcctagaaataaatt aaggattttt aaagcacata agacaatatt 27000 aactgaaggc cgggtgtggtggctcatgcc tgtaatccca gtactttggg agatcaagcg 27060 ggggcagatc acttgaggtcaggagttcca gataagcctg gccaacatgg tgaaaccctg 27120 tctctactaa aaatacaaaagaagtcgggc gtggtggtgg gcacctgtaa acccagctac 27180 ttgggaggtt gaggcaggagaattgcttga acccaggagg cggaggttgc aatgagccaa 27240 gatcatgcca ctgcactctagcctgggcaa caagagcaag actccatctc aaaaaaaaaa 27300 aaaaaaaaaa attaaattaaggattttttt aagcacataa gaaaatacta acttgggatg 27360 gtgtttaatt tttttttcaaaattctcaat tctgaattac acagtatagt ctcttaaaag 27420 gtttactatc catttcagtttacaaaaatt atgcaaccaa ggttttactc attgagtagc 27480 ttagactcta ggagaaaatgaaattgtctt tatagattca tttttactca caaatgttag 27540 actggaggca gagtgggaacagtctgagat gttttaaatt cacatatgca gcctattaag 27600 tcaatgttag attatcactggtagaaaaag tttcctataa taaaagccca ttaaattttt 27660 ttcttgtttg ttttggttttttgacatgga gtttcactct tgtcacccag gctggagtgc 27720 agtggcgtga tctcagctcactgcaacctc cacctcccag gttcaagcga ttctcctgcc 27780 tcagcctccc aagtagctgggattacaggt gcccaccacc acacccagct aattttttgt 27840 atttttagta gagatggggtttcgccatgt tgggcaggtt ggtcttgaac ttctggcctc 27900 gggtgatctg cccaactcggcctcccaaag tgctgtgatt acaggtatga gccaccacac 27960 cggcctaaat cttgttttttttaagtaaaa aactcatctt ttggggagat aagaggactt 28020 gctttaaata gctgctcactgaatatatgt gcacaaaata ccaaaatgat ttttaaggga 28080 atactcacgt atctccaaagaaaaactgag atgttcccaa tctgtttgat agaagattta 28140 ggcactcctt ggcatctctgtatatctaat aaggatgaaa ttatatctca aaagtagaaa 28200 agatactcta aaactacttttgaggaaggc atctctaggt aaactcaagt ctgcatttta 28260 tcttgtcctt tctctctagggcccttgctt gaagaataat ccatactata cctgtgcttc 28320 cacttctcgg aggtggtagaggggaggctg tcctctggtc aggagaatcc tattcagtgc 28380 tcccttagac attcttccaggcaggatcaa actcaaagga aaaggaattt gtgaagcaaa 28440 ccatggcttt gtcacagtaaagtaattgtc actctcaacc cagaatgtgt gaagctgcaa 28500 aagaagagaa aaaacactacaaggatgttc ttttattaaa tgaaagatca ctcaatcaac 28560 atgagagttt ttcataaatatctttatatg aagtgtcttc atacaaattt tccagttaga 28620 atgactcagg ataaaacaagattattttaa atgtgtgagt tttgttgact gctatcttcc 28680 aaacacccag aacaaggcctgacacatagc aggtgttcaa taaacatttg ttgaatgaat 28740 gaattacacc caattctatacatggagcaa aaagatccat ctcccacaaa gaatttccaa 28800 catgacattt tattttactttgttgtttca catactagtt gaatgcctaa cagaatccag 28860 tttcacagta atatgaaggaattaagatta acatataagt aaaattttct cttaagactt 28920 ttctcattgg tttttttctaaacagactat gtacatttgc aactaggcta ctaaaatact 28980 gtaatattta agaacactcaccactgcagg gagaagcttc tcttcgagga gagcaatata 29040 agccaatgta tctgccccttgttttgctga gagttcataa tcagcattat atttctatta 29100 aaaaaaaata aaccaaagaaactcagaagt caaagagcag cccaaagaca gtaaaactaa 29160 tcaaatcagg attcatatttttccttacaa atagcaaacg tggtattaac atgggagaaa 29220 gagaaataca aatccagtgaaacctattgc ttaatgcaca taaagttctt agcacagcat 29280 cacatacaca atatggtcaataaatgttac aatattaact actattattt tcatgcttgt 29340 taatgaccta gttcagtgcatggcatgtag tacaaacaaa gcaactatta tttttaacaa 29400 gaaggaaaca gttccaattggctgtgtttc tccccaagac aaaagaaaaa accttccaat 29460 ttataaaagt tcaaaatgttattgacacaa tttttttaag tacagtattg gcaaaacaaa 29520 taacttatcc attcatataaatctttacat gtttaaacat tttggataca taattctcta 29580 ctgttaaaac atttagatacattattccat aaaactgcta ttaagagctt tttaaaaata 29640 atatagttgc tagttggtgagagctcaggt gtcatgccca gcacaaaatg tgtaccaact 29700 atgtgacaaa atacacaatcctatcaataa gtttaatagt gaaaatggct aattaaatct 29760 acttacatac aaatttcaataaaattcaag caaagtttat taaaatataa actgactacc 29820 tcttcatatg actttcaaatcacagaactc atgctttaga gcagtgattc tcaaatctat 29880 ttttacatct tgtcccaggtatctccaatt gtctaatgag ttggcaaaaa aaaataaata 29940 aataaaataa agcccaacatgcagggatca acaatctacc aatctacttc ttcaaagtga 30000 accacccacc tgttttcttaaaaagtttag tatttttgct ggctgagaaa ccatgtcgtc 30060 ttcagttgtc aaaattggtacatcgcctat aatcaaaaaa cagtttacat acaaggacac 30120 agtgtgattt cctctgagagattccaatat cctaaaaact atagcagttg cacatttaga 30180 ggggaaaaaa tgagtaagcttgtcttggtt tttttgctgt ttcacaaaga gcggttctaa 30240 agaacatttt cgtttctcataatttccagt gatttcgctt gcactttgag gtattactca 30300 gaatgatcaa accataaacaacacagagcc aaggtcacgg taagaaacta aatcctcaat 30360 attatagcta tatacctcacagctttaaat ggaagaagaa aaaaaagagt gcagaaaggg 30420 gagtgtaaag gtttacactatacctcttga acctctccag gtgttatcta tcacattgac 30480 tttcaagggt gcaccagaaaatttggcata agcctgaaac agagtttgca atcaaacatt 30540 ttagaccaag tgcgtaatgcaaagctgcag agcagcttta cttcacagcc ccatccccca 30600 aaggcagaag gcgactgatactgaggccgc atccctcccc gtgatctcaa gtagggacgc 30660 gggcgccagc cacccggctttcgaggactt tgctccccaa aaggtcctgg ggatgcagag 30720 tagggagggc gcgccccttgcttcggggtt tcactccacc gcccagacag ctttgtgagg 30780 cggacaaaac tcggagccaagcggagaagc cttgatgtcg ccgccggcct tcggcggggg 30840 tatcggccag gttgggactcgtcggtggga gggagcccag ggcaatgcag cgtgcagcgg 30900 ctcggccctc ctggaccccgcagcgcagcg ggaaactggg gcaagtcagc ctggcgcctc 30960 cagccccgcc ccttcctcctgcgccctggc ccgcgaggcg gcctcaccat caccaccagg 31020 gactcgctgt gaaccgatgggagtccccag ccgcctcccc agcaactgag ttccaagggg 31080 gccgccatct tgcgcgggccgacctttact atcccggaag tacttttcct cctaatttcc 31140 ggcacgtgaa acgcgggggcgtggctaagg gagggaaggg taatttgggg gctcttgaga 31200 gatgagagaa acaacgactggagggattaa gaagaactct taggagcagc ttggcctaaa 31260 gggtaagagt tgtgtgccttaagtcagacc caccagtaat tcttcaaata ttaatattta 31320 ttagatccct agagtgttccaaaccctttg aacattaaat ctgtgtgatg cctaaattaa 31380 cctgtttgta aaacagggatgacccagtat ttacaagatt gtggtattta caatgtcgga 31440 gagaaagaac ttggcacacaggaggcactc agtaattgtt caaccccttc tcccatgtat 31500 gtggcctctg cctgcctttccagcctcaac ctgttctcac cctcatgttc actggcacaa 31560 ccctgcttaa aagttacagtatttctgaac gtcaggtaaa ttgtgcccac cttcagtgct 31620 ggtccttcaa tctggaatatctttcttccc tatgcagctg ctccctcaaa aatgagctta 31680 actcaccttc cctgtgatgtttttacaaag ttaggagctc tgggttcttg tagccccttg 31740 tgcatatttg aattgtagcttctctcccta gctacagacc ttttgccttt cacagaagag 31800 ggctagcttc ataggtttgggacctgtgca gccacacaag gctctgtgct taaaaaggct 31860 cctcatttgg ctgatgctcttccgtcactg tcttgaaatt cttaagtttt gaacaagggg 31920 ctctgcattt ttattttggactgggcccct caaatcatgt agctcgtcat gtctgtgggc 31980 ctaatgagca cagactaaatgtttcttgac gttaaacctg acagtttctc agaaaaacta 32040 ttaaagagga aattttggtcttggaaatgc tatgatccaa acatatgact gggtctcaga 32100 gttagaactt gggtgctgggtcttttttct agaaggcaca gtagtctttc tgggacaccc 32160 tcgcagtcat gcctctgagttctttcctct attccactga gatggtcgga tcttcttaac 32220 taaaactcaa aagaaacctctgcaactttt cgtaaaatta ctattcaaca aatatttgct 32280 aggcactccc ttactgtttacataatcatt caaattacag tgccagcctg ctaaagtctt 32340 ttttacatgt ttcaaaaccaagagttctaa gaaatgaaaa agagatatgt cagaaatgag 32400 aattcattaa taataactgtataattggaa aatgcataac atatgtacca ttaaaactgt 32460 cgaaacattt attgagcagctcctatgtct tagacatcat aaggttctgg aacaatacag 32520 aaatatataa aacatgaattctgtcctcgg gagtttacag tgcacaaagg agatatacac 32580 acaccaaggt gtaacaagtcagataatcac gagtagggtt agaaatataa gcaaaatact 32640 acagaaacac aaaggagggacagtgcatcc tctgtgtgat cagtggagac tatctgttga 32700 gtcttcagta taaagaaagtaaagtaattc aactttcaga ttgtaatgga acctccctgt 32760 tttgagggtt agaaatgtcaaaccatagac tgacaaaggc aaggcagggg gaaagaaaat 32820 cctaagccat aaagctgagggtccatatac tacattcagg catttaaaac aatcatacag 32880 aaagaatgtg gatttcctgagccttgtttg tagcaggact gagaggagtg aatgaacatt 32940 cttagtggac acataacacagttactcagt ttggccttct tccgtactgc tgctactaca 33000 tacagtgatt tcatttgcattaaagagaaa ttaaatctct tcccttagtg ttgaattgag 33060 acagaatagc atgtcattgcatctgatgaa agaatcctta cacacaggtt tattagtgca 33120 tagataatag caatcttttattgctgcagt tcctcttgag agtctgtatc tgttagattg 33180 aatttaaaaa actctaacatagctgattgc acaaacaggc tgaatactgt tccagagaac 33240 cacacagaaa attgaagccttcagaaatta tgcctggtat ggttagttgg ctcctgtgcc 33300 ttttcctggc tgttttagagtattgttcag agttcatcta aatcataact tcagattcct 33360 accgggaaga atactgtctgcaaaacatcc atgtttgtgt gggatggcct gaatcacagc 33420 tttataatga gtatcccagacagtgttttc cccaaaaaag aaatcttatt tttcaaaaga 33480 tgatcattaa gaatggtttcatctggtgat agtcatctga actcaggcaa cctgaccttg 33540 gcatccatac ccatccttcatgtgtttctt aacttgggag ccacagtgca aggtttgtta 33600 ggctcttttt gtctgggtgattaagaatct gggaaatggt gctgttggga aagatgaaag 33660 gtttgtgaca aatgatggtacagtatatgg tcccttatgg gaaaattaaa ttgtaaagtc 33720 ttaaatatcc ctttgaacttgaagattctg taatttgtgt tcagatataa aatccttaag 33780 gaggggaaag aacaaacactgaatagaaaa aaggagagct gagttcttga cttggcctct 33840 gattagccat atgcgcttgggccaatcatt ttacattgat acctctgctc attcatttat 33900 aaagcaaaag ttcttttatctctgagatgc tatcatttta tgtgtaatga acatttaata 33960 atttctgaca tttatggagcaatttctaca tgccagaagt tgtttcaagt atctaacata 34020 acttgcttta tctccataatgatttcatga cacaggtacc ttcctaaggt taactaacat 34080 ctttgtgtcc ctccttgaaaatagaagaaa atatcagtac ccatatagcc agtaggtggt 34140 agaaccaaga tttgaacccaggcagttttg gcttcagagt ttatactctt aaccactgtg 34200 tcatactgcc tcatgtagcctcctgtgtag tccattaaaa agcccagttt cacccaaagt 34260 ggagatatca atgccatgatattattgtta ttatttatgt aaataatatt tgtttagtat 34320 tacaccatta aacgaattcattaagcagct tctgtatgcc tagcacactg caagcctgtc 34380 agggaataaa aatttatgttcgcctgtaat cctagcactt tgggagggag gatcacttga 34440 acccaggagt tcaagacaagcttggacaac atgatgaaac cttgtctcaa cagaaacaca 34500 aaaattatag ctgggcatgatggtgtgtgc ctatagtctc agctacttgg gaggctgagg 34560 cgggaggatg gcttgagcccaggaggagaa gattgcagtg agccgtgatt gtgcaaaaac 34620 ttatgttcaa gcttttagaggcacagttga tgaaacaagg cccacatatt tgaactaact 34680 agagactatt aggaagtaccattttgtcaa gaagacaagt ctagtatggt tttagacaaa 34740 ggcttgaatg tactgcaatgcttggcaaag gcctaatgaa agaagatgat agctgatctc 34800 gaagggtggg tacgttgcatttagattggc acagagtaag gcattgcata tgggctgtgc 34860 tatataagtg aaggcatagaaacaggactg actatggcaa ggaatctgaa gatgagtaaa 34920 cgaatgctgg gtttggttataagctggaga ctgctcaggg agagtattgg ttgactaact 34980 tgagaaccag aattggaaggcatgggaatt tatagttttg agaaagtaaa gacaagtaaa 35040 agtccaaata caatattagtggatttacat agtcaaggat gggaggtaga cagctattgt 35100 tacatggaca ttataagaagaagcatttta taaacccata aactatatta aataaagtaa 35160 aagtgtggtg gaaaatgctgctgaatatta ttggaggaag gtcacttggc acccttccgc 35220 tgagcatttt tgatagaggaggttgttaga aatccagtgg caagggatta ctggagaaaa 35280 gggtataaac gataaggacaggtctaagag catgaggaat atgactgata aaggaagaag 35340 agaaatggga caggactgaaagactcaaag tttttgaaag agacgacatg gtgaatctac 35400 acacatatga ggacaaaattggctattatg agtaaaagtc tgaagatgct ggagagaaga 35460 aggatgggat cctagaaattgccctaagag ttgaggctga gatccggagc ttgagcagcg 35520 gggctggcag gcattgaaaatgagagacat gctctctttc tgacactgga ggaagtttca 35580 aggggaacta tgggatgctgagtagataaa tcagttacct tcaaatttcc ctattacatt 35640 agaaaaaaaa taaaagtgcctgagagaaag agattggggc tttaaaaaga cagctgggca 35700 cagtggctca cacctgtaatcccagaattt tgggaggcca aggcaggcag accacttgag 35760 gatcacttga ggtcaggagttccagaccag cctggccaac atggtaaaac acaatctcta 35820 ctaaaaaaaa aaaaaaattagctggatgtg gtggcgcaca cctgtaatcc cagctactca 35880 ggaggctgag gcaagacagtcgcttgaacc caggtggcag aggttgcagt gagctaagat 35940 cgtaccactg cactccagcctgggcaacag agtgactctg tcacacatac acacaaaaaa 36000 aaaggcggaa taatttagagactatatcag gggttaataa aaagtaaatg ggagcattgc 36060 tgacagtcgt aaaggtcaaatgtaaattag tattaaggcg ataaatcaag ggagagccat 36120 gggcaggcta atgattaggtgcctctttaa acagatattc tttaaatatt cattcagttt 36180 ttttaaatgg aaataaagttggccccagaa tgtaacaaga tcaatttata ttttaccaat 36240 tattttaaaa gttttatttggaaatccgga ttttagtacc acttaaaagt tggtcctgag 36300 gatgatagaa gagggatggtaccctaggag ttgccccagg aattgaaaga agatgaactc 36360 tgtagcttca gcatgatgggggctcagcca tcctgacgtc tagctaaatt tgtacagttg 36420 tgtggcttcc tctaaagatactttgttacc cagaagcata aaaagccaag ttcatgtatt 36480 tggtagctat gtgaaggtgatcaggcagta atatatactg atagggattc attttgctcc 36540 tccagcatag taaacctgctgtcttttgta tgaaaaagaa ttaaatgaaa ccaaaatgag 36600 aatattataa tctgacattgtgctgaattt atgttgagga aataggacaa ttatacttaa 36660 aaaatgaggt ttgtaaagggtcctaggtgg aaataaggac cttataatgg aagtaaataa 36720 tggaaataac ggaagccttatttctatact tcactcaaag tggtaaaacc taatatacca 36780 atagactgtg atgttaaatatgtatattat aatactgaga gtagccacta agagaactat 36840 acaagtgata tactcaaaaatactaaataa atcaaaatag tttttttttt tttttttaag 36900 tccaagtaac ccacaagaaagcaaggggcc agcaggggag tgataggttg gaagtgggga 36960 ggtagtcaga gagatgagaagttgagaata aacagaaaac aggtaacaaa atggcaggct 37020 taaatcctaa aataccaagacttattctaa atgtaaagtt gatctcaata caccaattaa 37080 aagacagaaa ttggcagagtggataaaaaa cctaagctaa atgtatgctg tccctcagaa 37140 acttccttca aacatgacattggtagatgg aacctaaaag gatggaaaag ttataccatg 37200 caaacattga tcaggaaaaaaaaagtagta tgcgtgtatg tgtgtgtgtg tgtgtatata 37260 tgaaggacat tacataatgataaatggatc agtccacaag acaacatatt aatcccaaat 37320 gtgtgcacca aacaacagtgcttcaaaata catgaagcaa aaactgatag aggtgaaaga 37380 agaaatagac aaatccataattttaactat aacataagct atagttatac ccgacactca 37440 gcaattgata gaactactggacaaaaaata agcaaagata tagatgaatt aaacaacagg 37500 atctaataga catttatggaatgccccacc cagtgacagc agaacataca ttatttttaa 37560 gagcctatat aacattcaccaaagtaggct attatgagtc ataaagcaaa cctcaacaaa 37620 agtttttaat gaaataatgcagaatatatt acttgactgt gctggaatta aactagaagt 37680 caataacaga aagataacagtaaaatctcc aaaaacttgg aaattaacat acttctacgt 37740 aatcagtggg tcagagaggaagtgtcaaca gaaaagaaat atatacaatt gataataaaa 37800 atacaacatt tcctattaagagaatgacaa gaagccacag actgggagaa aaaaatttgc 37860 aaaacatata tctgttaaaggacttgggtc caaaacatac aaataactct taaaacttac 37920 aacaagaaaa caacccaatcatactggatt aggatagact gcaaatccag tgattggtaa 37980 ctgatatggt ttggatttgtgtccctgccc aaatcacatg tcgaattgta atccccactg 38040 ttggaggagg ggcctaatgggaggtgattg gatcaaggag ggattccacc cttactgttc 38100 tcataataat gagttctcatgagtcctggt tgtttgaaca tgtgtcgcat ctcccctttc 38160 tctctcttcc tcctgctccagctgtgtggg acgtgcctgc tttgccttcg ccttccacca 38220 tgatcgtaag ttttctgagaccccctcagc catgtttcct gtagagcctg tggagttgtg 38280 agtcaattaa acctcttttcttcataaatt acccagtttc aggtattcct ttgtagcaat 38340 gtgataacag cctaataaaggtgaccttat aaggaaaaag agacatagca acacagacgt 38400 agacccagac ccaaaattggtgaaatctga atgaactctg tggtttgtac caatgtcatt 38460 ttcatgattt taatatcgtacaatagttac agtagatgtt acaattgggg gaaacatccc 38520 tgtacattta ttttacaacttcctgtgagt ctatacttaa ttgaaattag atgtttttaa 38580 aaagaattaa aggtaatgtaatggcatgtg atgcaaaaaa tgggcacatg aaatatccag 38640 agcattcaac cagatggcagctaaaaatgt ggaccatctg ccttcatgga actttgtatt 38700 tcggacatct tttcatcagcaagaccatgc catttaaatg ttttcccaga agtgatgctg 38760 agctatgttc agtggccacatggctctcta gaatcagtaa caatgaataa ggatggacag 38820 ccgggagtaa agatgctctccaggaaatca tcttaggact gggtaacccc actacacccc 38880 tgggattcag tcaaccttggaaatcactgg aatccttaat ataaactaca aacaatccag 38940 cattctctgt ttggctaaagatacatttca aagttttctt taagaaaaaa aagcaatata 39000 ttaaaacgct ttgtttgaatatatgtatat ttgctggatc aatagttctt acagccatat 39060 tactaaaatt aagggctatggcataataag aattactata attctaaacc cattaacagt 39120 ttatagctta tccatttcttatactatccc tgtgaggaat aattatatca agctttattt 39180 gtttacacca aacaggaaaaaatagatgta tttattcata tttcctccat tggattagcc 39240 aagtctacta gggagcaaaaatagtaaatt cacatgcctc ctttttttat ggtagtaatt 39300 gaaaaagtta tggatatgatcccattttct gaaatgttta aaaatgagtg tataaaatga 39360 tcttattttc agaacttaagtcattatgtg tattgtttat agggagttat attacttttc 39420 cagattaata ggctgccatcgataatgaag aacaaataaa tcctgccttg tgaaatgctg 39480 ccattttaaa atttgcctgaggaaagattc attattggag gattctttgc tttatacata 39540 aatcgcttta aacagcaaagtctcctagca tttaaaaata gtccatatat aaaaattctc 39600 tcaatccaca atcatgtcagttgtgtaaaa tgagatatat ctggtctgat acctcagttt 39660 atggaaattc ctcttgaaatgtcagtcatt actcacatca gtgctttaaa tgccatctta 39720 gcaaactaaa agaaattagcgtacattttg agtaatggaa gtgagttagg aagaggaaag 39780 aacagggtcc aatgagtttgaaatggtgtt tacagttggg tattgttcat agcttttgga 39840 actgtcttgc ttcccctattcatcaaggtg ctgaggcaac aacattaaaa tcaacccaag 39900 aagtatactg agtaggtatatgggagaatg gcatattaac ttcttgataa aatgtctcag 39960 tatgtaaata cttatgaattattattaatt tatattactg tacttacacc atttgattac 40020 acctgcataa agctgcatactcatttttac ttcaaatgca ggaactaact cttatatccc 40080 ccacagcatt tagttggcacataatagatg cttatagttg atggacacaa aacatattca 40140 actattacaa gaacagaacttttattgctt cttcagtttc aaatgtctca aagttaccct 40200 ttgcttttga ccacaaagtctacccctcct tatggtagtt tcctcaggtg tctgtgtcct 40260 ctctttccat tgcattttcttctcccacat ctacccagta actggagtct tcctcctttt 40320 gctgcccctt gactacaaggcatttccatc ttttggcttc ctaatatgtc atgccttctg 40380 ttattgccac cacagccttccatttcccag ctctcatttc catcctaggg atgggacagt 40440 aaggctgtca tgaataagcataatttatat gcctttttct ccatttgttg gtatggataa 40500 ttaagtgacc ttcaactttcattcaaggtt aatcatagcg ctgaaaacca aaaggataag 40560 atgagagtga gcaaatcaagtccagagagg tgtatttcca gtgtggaaca gtaactcagt 40620 gtaaatatcc aggagagaggcttccaagag agcgtatgtg tgtgcactgg tgaggggagg 40680 aagggttacg ggcgggaattgtggtagaag cttcctgagg ccaaagaaaa cattcccaga 40740 gggtctctat tttaaagaaaagattttctt tctctgattt tttttatatg tagatgtctt 40800 cattatatat ttggacctgtttttaaaaca gcttagtttt agatttattc ttcgagataa 40860 cttgtaccta tggacatcaccaacagctta caaaaattag accagttgac tctcaataag 40920 acttctcacc tgatccatgagcatcatctc tttatttgga tttcataagc cagatgatat 40980 cttaaaattg gcaaatataattacaactcc gttgaacata tttttctcca gaaaatgttt 41040 tgagtacaaa aacgacatctagaggtgact gtcaatatat cttttctttg cacgtactac 41100 atatttgttg ccgtgttttagaaatatttt agaagtttgg gtaacagaac cggcagtggc 41160 atttattgaa ttcttcctcacaataactga agggatagta ttatactcgt tttgcagata 41220 taaccctttc aatccaactaatatttatta agtttactaa atgctggcac cagtgatacc 41280 aagatcaatc agatgtagctcttgttactg aatggcgtac atggaggaga taaacacagt 41340 aacgtccact cttgtgtggtgagtgctgtt ggagattaat taagtgttat gaggttatag 41400 aagaggaatt attcacccatctgcagaggg cttggggatg aaacagtgtc agtgaagcac 41460 tcctggagga ggtgtaccaggggaggtgtt ccagacagaa aacagctgaa gtaaaaacat 41520 ggaatagagc aatagtatattgtggtctga gaactacgta taattcaaca tttcaggagg 41580 gtaaagtgag cggtagataatgatgagaaa taagactgca ggcacagtca gaggcctatt 41640 tacccacaca aaggaatttggagttggagg caccaagggt aaatatcttg tttcaaaaag 41700 tgattacatg aggatttttgcccagttctg tttgtctcca aagccatgat ttttacatta 41760 aaccatattt acttcatagacattcttcaa attaaccatt taccaaagat gtaaataagt 41820 tataagaaga aaaacaatactaatttggaa gataataaaa gcattatttg gaaaaagcca 41880 gtgtagtatc tctattttcaatttgaactt tgagtccaat gattagacat tttctagtcc 41940 catattttat accataccatgcagtctgct tttgttaact atcattaata gtgaaatgac 42000 aaactgacca gcatctaatactggggttac actggtgggt accctacaac aggcgttttg 42060 ttctttggta tctaaccactaccttcagta gctgcctgac aaagatgtga atctgaggcc 42120 acactacctc aagggcacctctaccaagac acatctgctg ggcctaatga aaacagaggt 42180 ttctcctagc cctagcattggagactgaca taccctcaat ggatgtcagg ggagacccag 42240 tcactaagga tgactctcctgcccctaacc cattagcatg tcccaaaaag aaacaggaga 42300 ccaaagacct ttgccttgtataacagtcat gctgcctcgg aaaagctggg aagtcccctc 42360 ccagtgaccc catgctctatcctaatgtga acgatgttca tgctttttca tcaaatcttt 42420 taaatagaaa gggtgcagagaaagtcaact gtaatcactt ggtgcagagt gcagggagag 42480 agagcatttc atattcaagaagaaattgtc caagcagtaa atggcattgt ggtgagtcct 42540 gtaattggag atagtagagaaacactggac agctgaggaa actccagaag ggagaaaaaa 42600 aaaaaaaaaa aggaaaacctaagaacaaaa taaaagagtc taaaatttac acagaatcag 42660 aaaaagactt ctgaacaaaatagcattgat ggtgctggga aacttaatta gtgcataccc 42720 cttacagcag gaggtgggtagaggaactgt tatgttaacg aaagatcatt tgaaaaaaaa 42780 aaaaacaaaa acatgttgtctttctcttta aaattttaac taaattccct gattcttaac 42840 agaacaggga atatacatttttcactaagg aacaagtgtg ttttcttaaa attccatatt 42900 atgatctgtt acaggtgagtgggtggcaag tagaatattg tggttaactc ctgggatgga 42960 gaaagggtag gggaatcgatattattaaga gagggatata ggagaggctt cagtaatttg 43020 cagtttgaat actgcaaacttaagtagtag gaactaatgt ttctggagtt attccttata 43080 cctttataaa tgcctaaatactacaaacat ttcataaact tttaaaagtg aattggagat 43140 tcgggcaata tttagggaaatgagagggcg caagaaagag atcagagggg cagaggatga 43200 gaagaagagg agaagtaagtagggagagag agaaaaaggg aaggaatggt tttgaaccta 43260 gtgagctttt ggaagggttaggttagaggt cacatcatgc tgttttcctt atcaattctt 43320 accctgattg agtagctctctacctgtttc tcaccaaatg gttcaagcag ctgccatggt 43380 attctcatct ctacttcccagccacaaaac agctccagcc aacataaagc agttcttcca 43440 gtagaaaaaa gaaaaaaagagcacctgcaa ttccctcaag aagaaaagaa caaaaacaat 43500 gagtatatat gtatgaatgaacctaaagca gctctaaact actccccttt ggggacagaa 43560 gcagatgcct tcgccaaattcaaaatgaaa tcccatatct ctgaagaccc ccttcttatg 43620 ttactgtata atttacatgcaagttgtctg catagttaat cgtcctctat agcatctggt 43680 atttatcatc aaaagccgcttgttgagagg ttctggagaa gctcttcatg tatcccgggt 43740 tttaaacatt tttctacaagtctgtttctt taaaattaaa agtgtagctg agcacagcga 43800 ggatctggta gtaagtagtagggagggtca ttcatacttg ggatgttgct aatgtgacct 43860 gcatgattca gggccagagaggtgaatgaa gataaaggga actaagcgtt acagccaaaa 43920 gggttctgaa aatctcctagtcagattcct cagtttccac atttgacagt agctttcgca 43980 gggaacccca tccaccatgactgagctgaa actagaagcc aggtttttca ggtgtcattc 44040 agggttgatt tagggttttggttttctcct gcaaaatgga gaaaatggga aaattttaga 44100 ttatccatgt tagaaggatgatgaatgtgt gtaaatgata ttgttcagat tctgtgagtg 44160 ggaccagcac catattggactggcctaggg aagtcaggat tctgccttct aggtaggtgc 44220 tcccatctgt tggtgacctggaacatctct ctgtgttcat tctggagatg cagatctggg 44280 tcccgcagac tcagacctttgcaggacact agggtggccc cttgttgctt ctcatctgct 44340 atagtctcct gccactgtggcctacgggtt tgatttagaa attcaggaaa gactagaagc 44400 aagcaaacag ataaaaggttagtgaagtgt ttatccacta gagcaaagcc acaacttttc 44460 gtattattta gctcttattattctaaattt taaattgttt tttattgttt cacttaccat 44520 gtgtaacttt cacttttcaattacacatgg caagtgaaag aataaaaaac aatttattta 44580 ttttttattt ttattttattttttgagaca gagtcacgct ctgtcaccca gggtggagtg 44640 cagtggcgtg atcttggctcactgcaacct ccgcctcccg ggttcaagtg attctcctgc 44700 ctcagcctcc tgaatagctgggattacagg cacccaccac cacacccggc taatttttgt 44760 atttttagta gagagggggtttcgccatgt tacccaggct ggtctcaaac ttttgacttc 44820 aggtgatcca ccctccttggcctcccaaag tcttgggatt ataggcatga gccaccacgc 44880 ccagccaaaa aaaacaaaacaaaacaaaaa actaaaattt agaataataa taagagctca 44940 gaaaatgaca gtaattaaacaaaggtcaca cggaaaagtt gcccttgata tatcttggga 45000 cagggtgggt tttccttattgttcttagct ccttttgtta atgtttattt tagtgcctta 45060 atgctactaa acagaaaattcagaaaacat tgtagatgtc ttccttttac gctaatattc 45120 tccagctttt catacatttgcaaaaagaaa tagattatgg atttctcaag gtttagccgc 45180 ttgttatttc cgtgtgtaatacaaataagc atagttaaag gttaatttaa ttagcaaatt 45240 tgcagttatc tgaattaagtgtactccaaa tttagtttac aagacctgac aagtttgtga 45300 ataagttaat tcacttctaatgtattttaa tatacccaaa ccacaaagca tcttaaactt 45360 tgtaaaaaga gtgtgcacggaggaatgagc actagatgtg aattctagag aactggggtc 45420 caggctctgc tgtgtattggcctgagcagt tcacttaact tccctgagtc ttggtgtcct 45480 gttttgtata aaggggaacctgatccctct tctgcctggc tggcagagca ctgtgagact 45540 ctggagaaat gacatatgttagagatgttt ataacctgga acacggtaca cggtatgttt 45600 tgtttgtggc attttttttttttttttttt tttgagacgg agtctcactc tgtcgcccag 45660 gctggagtgc agtggcgcgatctcggctca ctgcaacctc tgcctctcag gttcaagcaa 45720 ttctcctgcc tcagcctcccaagcagctgg gactacaggt gtgcgccatc atgcccagct 45780 aatttttgta tctttagtagacatggggtt tcaccatgtt ggccaggatg gtctcgatct 45840 cttgacctcg tgatccactttcctcagcct cccaaagtac tgggattaca aacgtgaacc 45900 accatgcccg gcctgtggcattgtttttaa actaaagcat gtattatagt tttcctgaaa 45960 agtaaagatt tccagaaaaggaattgggaa actcttttac cctcatagtt ataaaatatg 46020 gaggtttcct tcctccaggttggtcagctt ccctcttgat atggtttggc tttgtgtccc 46080 cacccaaatc tcatttgaattgtactccca taattcccac atgttgtggg agggacctgg 46140 taggagaatc atgggggtgctttcccctgt actgttctca tggtagtgaa taagtttcac 46200 aagatctgat agttttatcagggatttccg cttttgtgtc gtcattctct ctttgcctgc 46260 tgccatccat gtaagaggagacttgctcct ttttgccttc tgccatgatt gtgaggcttc 46320 cccagccatg tggaactgtaagtccaatta aacctctttg ttttgtaaat tgcccatcag 46380 cagcgtgaaa atggactaatacacctctgc tctagttctt tttggaacta ggcctcttcc 46440 ctttggttgt cttgccactcactgtcagat tggacacttc tccacactgg acctcaacat 46500 caccctgctg gcagtcccacaggagttaaa atgtctgaag agggaatggc ccaaaaagga 46560 gtctcactgc cattgcaagagctggtttag aataaagggg gcagggtgga tgatgaagga 46620 ttacttaatg ggtacaatgtatgttatttg agtgatggat actctaaaag ccctgacttc 46680 cccaatatgc aatctatgcatgtaacaaaa ttacacttat accccatata tttatacaaa 46740 taaaaaggaa ttttaaaaaagaataaaggg gtatgctgtt cattcattta ataaacatgt 46800 attgagcacc aactatgtgccaggcactct tctagatgcc tgaggtgcat aaatgaacaa 46860 agtggacaaa aattccttcccttgtggagc ttacactcaa gaatatgaga ccaaggagat 46920 acagtctcag aaggagcagagacaaggaag agtcaatgat gcccagtgaa caatgggata 46980 aagcttagga gtgttcatcctcattcataa ttccatagcc cataaacgtg cagacccaga 47040 cttgaccata atgtgactagatactaacag ggagtaatta agtttatttt tttcatatga 47100 atttcacaga catatacaatagcatggtta aaagaactaa attaatttta tggtagtaat 47160 tttttccaac tctgatattttgtgtcagct cctacttgtc ccagccatgc ctatctgtgg 47220 tctgcgggcc ttcaaagggcagcctttgtg ttgcccaaag ccagcacagt ggtgctttgt 47280 gcaggggcat ccctgtgcctcctggaattc gtctgcccca tgaagctagg tggctggtgt 47340 tcgtggccag tcctctaagaagcaggtgtc cccactaccg tccaccatgt gtgtcctgtc 47400 tgcagaacgt ggctactactcagatcctgt ttctcactca agttctacct caaggctcgc 47460 actctgtcaa gtgatgtacttcttggggtc catctgcctc gggggtggaa attgtgaata 47520 gaactgtagc ccagagaatcattgagagac taggtataag ccagtggcag aacctcaacc 47580 aaaatgccgg gcttgtgacccctagccctg ggctcgtgca cataacacac agtgctctga 47640 aatatcattg tttggggcattattgtcact tttcttctac tgatgtggat ggcatgttgg 47700 attttatgaa tatcaattattcagattctt ctcaaatagt aattagttgg ttttgagagt 47760 aattaaggca atagatgctcctacaagaaa agagaatgaa agaccctgat ttgtgtattt 47820 ggggtaagat cttacaacaaactgggccca ggtctggaat aaatgtggac aacttctagc 47880 acagcataaa agcattaaaaagtttattaa cacatcatgt cggtgtcaat gaaaatcaaa 47940 ttggtataat tttcaaagaagaaactaatg atactttaag gctgtgttta tagtaatact 48000 atctttctga aagatactttgtttatgtac tatattaaca tagaaaaata gtaagcagtt 48060 tccagctaca atcagtggtttaaattaaga tgtaaatgtg ggtctaccag tgagaaacta 48120 aggcaaatgt ctccatctaagacagagttg ccagataaaa tataagatga tgcccactta 48180 aatttgaatt tcatatgaataacaggtaat tttttcttat aagtaaaaat ttcaagctta 48240 accgggcttc ccacattattgttttttttt aattttgttt tgagatggag tctcactctg 48300 tcgcccaggc tggagtgcagtgacacgatc tcgactcact ggcttcccac atttttattt 48360 gttaagtatg gcaactataatctaaaggac aaaatttgct tccaaacact gcccctgaat 48420 aagatccaaa gtacatatgatagccaatta atttggaaaa tacctcaata caatttcaaa 48480 tctcacagag gcagggtttctcaacattgg cactattgat tttggggcag ttaattcttt 48540 gttgtgaggg gctctcgtacattgttcaat gcttaacagc acccctcgcc tctaccacca 48600 gatgccagta gcataccctggttgtgacaa ccaaaaatgt ctagacattg tcagatgtct 48660 ggtggggagg gggtcacaaaatcatccctg ggtgaggacc cctgttctga gggaagaagc 48720 ttatttataa gtacagtatatgtattaata tcttagtaga aaactaactt gtcctgatct 48780 tgaagaaaga agaggaaagccatattttat gtagccttta ttttaaaata tcatgggaga 48840 agcaccaatg gaaatggctgaggaactaga gagctggtag acgtgggatt attttgcagt 48900 ctgaggctct atgacagaagcttctgtttg ctccagcagc tttgagagaa aaaggcagta 48960 gaaagtactc atgacacggctcaggttccc aatatagact tcatccaagg ctttgaaaga 49020 acagttagta cagagtctgctgatttctgg aaaaggagtg ttttatttgg atagcaataa 49080 ctgaaattcc aacctagaccacctagctga ataattatat gtgtgtgtcc aaaaaacaaa 49140 ttgccaagag atgcattgagaatcagaata ggaaaactgt ttactgtttg atttttcaga 49200 aaataattca acagggaattttgctaaagt gttgttcctt ctattttacc caagaaaaca 49260 tatgaattta ttttgctgtctaaaccaaga aactaatgtg gttatacata ttatggggaa 49320 gtttttaagg aattaacgtttagagtcttt gtggctctaa ttactgctct tcggttgtgt 49380 agtgagagtc actaagcattccttcctcca aggaatttcc agcataatcc tgttttccac 49440 tcaaattctt tgtgattgccagattggagt gaaaaatcaa atctacttcc aatgaagaaa 49500 aactcaaaaa aaaaacagtgattcaaacaa gtttccaaag agaatacatg aacttcttat 49560 tctgccttta ttaattgacatctacaattt tgtatttttc tcaggataat gcaaaaaaaa 49620 ttcttttgaa tcattctcactaaatttagg ggccaagtgt cctagctata ttgtttttta 49680 aaaaaagtta tgttaaagcagccagacttg gtggctcaag cctgtaatcc cagcactttg 49740 ggaggaggaa gcaagaggattgcttgaagc caggagttca agaccagccc aggcaacata 49800 gtgagaccct gtttctacccacaccctacc ccctaaaaaa aattagctgg atgtgcctgt 49860 agtcccagct actcaagaggctgaagcagg aggattgctt gagcccagaa gttgaaggct 49920 gcaatgaacc atgattggaccactgcactg acaaagcaac tcagttctga tctacagttc 49980 attcttggat aaacataatacacaacacct agaaatacag caaaaaccca ttacccttaa 50040 gacaaagtcc agaatccttactgcagtcta caaggtccta aattatctga ttggagcaga 50100 ctacactttc cccaactccaagttatatgc taagctgctc taagttagag atcaaaaaaa 50160 tagagtggct caaatgagatcaaagttgat aattctctca cctaccaggg tagaagtatg 50220 cagtggtcag tggttcagagtttgtggaaa gtttgatcca tgaagccatc taaataaggt 50280 actttgaccc aagtactttctatcttgttg cttcccaccc tttaagacat tctcctcatc 50340 cacatggtca aagctcactcaccactaccc tcactctgtc ccaatctgta gaaaggtagg 50400 cagagaggcc agcaaatagctttcttttaa atttgtgttg ctgggtgggt cccccaggaa 50460 acagactctg agatggagattagtgtacag gaagttcact atggggtgct ccatgaatta 50520 tcacccactg gaggaaaggaaggaagcaga actgggaaga tggaaaagtt aagctgcagt 50580 acagtcacaa caaggtctcatccaaattga tacggggcgt tgaagctgag ctggtccttc 50640 agagctggga tgagaccagatattggctgc aagccattcc aggaaaggga tgtgaccttg 50700 aatgagatga cttttcttagctgaggcaat tctcaaaggg agctgtcagc cagtagcctt 50760 gcagttggac agatggcagcacagcagagc atctgggaca ggatgtgacc tggaaatagc 50820 attgatatgg tttggctgtgtccccacccc aatctcatct tgaattccca tgtgttgtgg 50880 gagaaatcca gtgggaggtaattgaatcat gggggaaggt ctttcccatg ctattcttgt 50940 gatagtgaat aagtctcatgagacctgaca gttttaaaag ggggaatttc cctgcacact 51000 ttctctcttt gcctgctgccatccatgtaa gacgtgactt gcttctcctt gccttctgcc 51060 aagattgtga ggcttccccagccacgtgga actgcaagtc cattaaacgt ctttcttttg 51120 taaattaccc agtcccatgtatgtctttat cagcagcatg agaacagact aatacaaggg 51180 taaaccactt ccattcacccctcattatct agaatgtcta gaacagccac catagttgcc 51240 acggaggctg ggaaatacagtctctagcta ggaggcccta tgcctagcta gcactaaaag 51300 gaagaaggga gaagggatactggggagcaa tcacaagagg gaaggaaaat cattctttga 51360 tttgttcagc ttattgactgttgtagatca aaaaccactt cattgaggaa agcaacccac 51420 caaacacaga agcagaaagagagctaaaaa tgggataaga acaactgaga aactgcttat 51480 ccagtaatat attttaaatagtgaggacta caccaaactt aggtaggctt cagaatgagc 51540 cagggacaac tccccagaactctgcaggaa ggggctcctg aggacacacg tccaacctgc 51600 acctgaaacc ccaagtcacaaggacaactg atatttagat gcttttcttc ctaagacctg 51660 ctacatatca gaaaaagtcacttaaggtaa aagataaaat atgctgagaa acgattctgt 51720 ttcttaaaaa aaaagaaaaaaaagaaagaa agaaacccaa acagtaattg tattgaccca 51780 agaaagcact attaaaatgtatcactttct gttagtgttg cttggatttt caacataata 51840 atgttctgtt taaaagaaggagaccaaagt atgcttatta cctgaatgac agaatattct 51900 gtgcaccaaa cccccatgacatgcaatttc cctttatata acaaacctcc atgtgtaccc 51960 ctgaacctaa aataaaaattaataaataaa taaaatttaa aagtaaaaat aaaaataaag 52020 gaaggagacc aagaaatacttaagggccca ctccccttct ttctgaacaa aatagccaga 52080 ccagcctctg ccactgattgaaccagggat tggaaactaa cataaagcag ccaccacctc 52140 taggatgagc ataagattatcagtagcctg tgaggaaact tggtaataaa actctgactg 52200 gtagtgacac tgtatgaggtagtgtgggca tctaaccaac caatgtggtg gtcctcttgt 52260 ggacaggaga gggtatgtggtgtgtacaac aggaaaaaca cattaaaact ctagaaaccc 52320 aaacagaggg agtaggaggggccataaaat gtctgacttc tcaaatggct gatgagctcc 52380 tagaggtgtc cttggtctcttccccagagg caaatttcaa ctagaggttg aagctaagaa 52440 gcttatcaag actcagggcttctcactttt cccctcacag gcccactcca aggctctgat 52500 ttcctgggct ctctttgccttggcttcctg gccatcagtg tccatcactt gagacaatct 52560 gagtgtcccc acaccaaacacctgaaatac acctaataag aaagcctcat cccatctgca 52620 aagtatgctg gaactaagatccagattcac aactcaggaa atttcactct acttaagtag 52680 tggcattcta tcttcagattaaagtgtgtt tgaaaatctc tacccctact gactggggct 52740 gtcacacgag gtcccacttggccccgtctt gggactctcc tgcacagcac ttgactctgc 52800 tagacatcct ctctcctctcccctgtgccc tcatccccat catctcccag aaactcagtg 52860 ctggaacaca tgagacttctcagctgcata tcggcaaggc caagggatcc tcaaaacaag 52920 gcaagtttct gccctctcccgccatcaaaa gtgtgtaatt tctttgtttt gttttttgtt 52980 ttttgttttg ttttgttttgttttttgaca cagagtctcg ctctgtcacc caggctggag 53040 tgcaatggcg caatcttggctcactgcaac ctctgcctcc tgttttcaag tgattctcct 53100 gcctcagcct cccaagtagctgggattaca ggtgcccacc actacgcctg cctaattttt 53160 ctatttttag tagaaacaggtttcaccatg ttggtcaggc tggtctcgaa ctcccgacct 53220 caggtgatct gcctacctcagcctcccaaa gtgctgggat tacaggcatg agccaccatg 53280 cctggccaaa tgtgtgtaatttcttaaacc caaaggggtc tagccaggat cctgcattgt 53340 catattttaa gaataaagtgatatctccat gaagggcccc actttgagtc ctatttatgt 53400 ttgcctctgg gatttagctgtagctgggca tgtgtgatag tgggtaggtg acagcctgcc 53460 cagggtatat ttgcaccctggccatgtagc atggggttga gaaagcaggc tccagtattc 53520 cacaggtctg ggtttaaaccccagctctac catttattag gtttgtggcc ctggaagagt 53580 ctgtatcccc tctaagacttcatctcctcg tttgtaaaat agagatataa taatactttc 53640 acaaggctat tgtgagaattaaatgatgta taattcgttc ttggcatagg gcctaaaacg 53700 tagtaagcac tgataaatgacagttctaat agtagatacc ccataactaa gcgtctttga 53760 aggaagttta ccataaggtaagaagaaagt caacttgatc cagtaacaaa accccagaag 53820 gagagattcc agacatagcttggagcccag ctcttggcta tgtctccttg cccagcttgc 53880 ctgttattca tcactgagtcccatgtagag gtgggattgt aagtaaattg tgaaaagaat 53940 ggtttcagta aaaactccttaaggctttgg gatacagcac agtgttgtct tgataccacc 54000 accaccactt cctacaatacatatgtccca cctaaccagg aaagagataa cgtctcaaac 54060 tttgctttaa atgctgcccagctttggaat ctcacagtgt ccaaagcatc agaggatact 54120 caatggatgt aacggtggcctccaacagat atgaccatgg tcttcaggtg atgtgccaat 54180 ggtatgtagt agacacagctctggttccca acagaggtac tgatgattcc agcagtcatg 54240 acagtggtcc ctcgcagatgtggtgggatc tctggaacaa gcagaataat gttctgtgga 54300 tacagcagga cccttggaagcatcaacatt ctcagactga ttgagatgtt agggagttca 54360 gatatctctt aggctcagcaaaagtggtac tcgagcccaa ggatgtggtg ttagccagta 54420 gttgggggaa gagagggagaaagtgtctga taccatggag tagctgcagg tatttattta 54480 tgaccatgtg tgggttatagcccaaggtac agacatttgg ggtatgagga atgtgaagaa 54540 gagactttga attggacaagagtgaggaaa atagccaagg aactatggag tatacttcta 54600 ttacctgggg tcaccataagcataaaacta agagcttcat ttacagccct gtatttcaat 54660 gagaaagtgg aaagagcaccggaccagcag tctggagaac caggtgttaa tccaagtctg 54720 tcacttaatt tcttcaaaccttattgtgta tagaaaggag gaaaaagagt agcactcaat 54780 aactgacgtc ttttctttgataattttatt tcatcactac cagattcaga taaagccaaa 54840 gtcttgctaa cactccttgccagttgaaat gtggtgactc tcaaaaactt ggaaatgtca 54900 ccattctggt aagaaatcttttaatggaaa ctttacatct tctaaaataa ttaacattca 54960 gtactcaacc ttagaacattttccaaattt agatgagaag agatgaagca aggaaaaaag 55020 gtgtaaccct atcatacttttccttttggc gttgaaataa atatccagtt aggggatggg 55080 gaaagttttg gtttctttagtactgagttg gtggcagcaa gagctaagta ggggctgagc 55140 atctcaggta tgggcttcccttagatgatc cagctatcag gttctgccaa ggtgagtatg 55200 cccttgtggg gcttgcaaggcctagtcatg gtgggagacc ctctataaag gcctacaagt 55260 acttagggag gtaagaggacaccccctcaa gggctgaagc ctgtctcacc tttgagggca 55320 gtgagatcac cagtatccctgtgagctgta taacaggaag gataagtcca tgttctggtg 55380 ccgatcccta tgccttttggaacatttctc cctctgataa gaaacctcct tataatactt 55440 tgtttcactg gaacaacatcatcctcatca ttagtaaaat gattgctggt tgtcaacatt 55500 cacggagaag gtccctggggaaggtcagct gttgcaggga agactggcat gctggctccc 55560 aaggcaagtg ttcctttccatgatgaaggc accagggacc agggtcaggc cttcagtctc 55620 agagatgcag aaactcttgtcagtgcctga aagaagctga agaataataa gcagactgcc 55680 aatgttgtca ttaaaaggcaaggtatcttt tacgaggtga gcattagcat cagcctggca 55740 agtacattct tagacaaactgcacggaagc caatttaaca gaaagttgct tggcaagcaa 55800 caagggtgat gtttaatggggtcagcctat aattacatga tgcatccatt tccaccatgt 55860 gtttctgtct gcctcttggaccagaagggc tgtctttggg gccaggaaga cccttccctg 55920 gacagtattc acgcatctactgccagactt tgcctcactg actcttcctg tgtcacacat 55980 tcccaaacct caatgactcaccaattccct ttctgattga catggtcttg ccttaaacgt 56040 gttgatgtct ttaatcaagacagattaaag tgaagtttct tttttctgga agatgtgact 56100 gcatactgtt ttgaccgattatatgtgctc agctggcaaa gggctatgtc ccttcatagc 56160 ctaattcttg gggtcaccccattgttgcct tcctcccata taacacctac actgtagtgt 56220 tgaaatgtca tcatgttttattttatttca tttttatttt ctgtgttctt tttctgtgtt 56280 ccactgattt ttttgagtgggattggtctg cgaaatggta ttatttttag cacaaaatga 56340 atccagtgag acaaaatcttgtaacaaatt caactgtttc actgtatgaa agcagggcag 56400 gttctttact gatgacaatttctgtttgag aaaccaaact gagttcttca taaatgatga 56460 acaaataata tacacatattattgtattta ggattatagt attgctagcc ctaaaaaatg 56520 atcaatgaag tattggcaatgtgaggtgat aattttttgt ttctcctttt cataatagat 56580 tttgcctgtc ctgttagtgatttgcactgc ccgcagctac ccaaaacctc aagcatagac 56640 aaactgctta aaattccaacgtttgatgaa ctgattcaaa acggatagat taacccatac 56700 cacactccct gtgctgacactaatgaaaaa acaaaacagc aaaagagttt ccacttccca 56760 gatgtcaatc ttctatttgctttaatcatt ttatatttat ttattgatcc attcaattat 56820 gttggtttat tcctacttaagttgttccaa agcatttaag acagatgata agaacacaag 56880 ataaaaatca ggatcaagaaaaataaaaat aaatgttaga tgacaaagat gggaggagga 56940 attagaatta caaagatgtgtagaatgcta gttcctacat aattattaaa atggaggcca 57000 atgttcaagc tgggcttcctggtggcaaaa gcatacatga aattataata ggtacaaaat 57060 aataccgtaa agaaaacctccccaaataaa gaaagctttt tcttggcact cgaatctgaa 57120 agaaattttt tatttagataagaaaatctc agtgatatgg ataacacaat accacaatac 57180 aatctaacaa catgcaacccaacaaaatgt gtgttttttt aaggaatttt gttctttgct 57240 caatttcttc catctactaatttttaaata atgcatcctc tgtctgttca tttgaggtta 57300 acccaaatat tttaatatttaataaaatct aagtttaaat ctttttacct gcttctgaaa 57360 aatacaaggg gattataagactaatcaaat tgcttcccat gccactttat ttgttgtttt 57420 agttgagtgt gttagttctattttgctttt taattctaca aattaaacac tattattatt 57480 gtcatcacca ttgttttatatggccagtgt tgaagtgtat ttatttataa atgtctttgc 57540 tcaccatttc ttcttgaatttcagactttc aatttggatt cattttctgt gagcctcaag 57600 tatatccttc gaaattttcttttgtgagga tctatactca gttttgtttg tcttaaatgt 57660 cttttatttt actcttattcttaaacgatc tttttactaa tcgtgggatt tgggattgat 57720 cttttttttt ctttgacacgttgaaaatga tattctggct tccattaatg ctgttggaaa 57780 cactgctgta cctctaatttttattccttt gtggtgattt gttatctttg gctactttta 57840 agatagatcc tctctttgaccttgttgttc tgaacttcca ctctaatgtg ccaaggaatt 57900 attttatttg tatttatctgcttgtgattt ggcttatcat ctttcagcaa ttctgaaaaa 57960 ctctcagcca tttttccttggaatattgtc tcttttccat tctttctact gtgtttttcc 58020 agagctccaa ttaggtgtatggactttttc aaaaactctt tctttttctc ttattatatt 58080 ttgaaatctc atttctctgtactatatttt atatgatttc tttagattta tcttatactt 58140 cactagttat ctctttgtgtccagtctgct gtttaattca tacattgatt ttaatttaaa 58200 ttatatttcc atttacagaagttccagttc aaatatactt tttttttttt tttttttttt 58260 gagacagagt tttgctcttgtcacccaggc tggagtgcaa tggcatgatc tcggctcact 58320 gcaacctctg cctcctgggttcaattgatt ctcctgcctc agcctcccta gtagctgaga 58380 ttagaggcac ctgccaccacacccggctaa tttttgtact tttagtagag atggggtttc 58440 accatgttgg ccaggctgttctcaaactcc tgactttagg tgatctgccc acctcggcct 58500 tccaaagtgc taggattacaggtgtgagcc accgtgccca gcccaaatat acttattttt 58560 taaacatttt ttatttcttgtcattatttt caaatgtctg ttttatttct taaagcatat 58620 taagtatatt tattgtatagtttacttctg atatttctag tatctgcaag tctgtgaaag 58680 tgtgatgttg ttgtctgctatttcctctgg ctcttacttg gtgtgccttt tgcctagtat 58740 actttgtctt tgtgtgttgggaggggagtt catgttcttt agaatgttat ctgcaggatt 58800 ttttgagaac tggttaaagttggtgtcctt cagaaaggat tttcttttac ttcgattgcc 58860 tgggaggact accagttctctctgtgattg tttttttttt tttttcttgt catgctttcc 58920 acttccactg catttctcactggctcaact atagtagcat gaatttggaa ctacaaaacc 58980 acaagaagca acatcaactatgaagtatca ttgataaaaa tatttaactt gaatctaatc 59040 atgagaaata attgaccaaatcaagactat aggacagaat ctaggacatt ctatagagcc 59100 tggattcttc aaaaaagtcatacacacaca cacatgtacg tacaccttaa tttataagat 59160 agaaatcaaa agaaactacagagatgaaca gcccaatgca aggcatgccc ttcattgatt 59220 tctggatttt tttaagtcataaaagatatt tgggagtcac ttaagaaaat tagaatgcaa 59280 actgtatatt aaatggcattatggaattat cattactttt cttaggtgag ataaggatat 59340 tgtaattata caagagatgtccttcatcta ggagatggct gctgaagtat tgaagggtaa 59400 agtgtcctgt gcttataactggctttcaaa cagacaaaca aaaaaagctg gtccaaaatg 59460 gcagtggcaa tggatgtggataccccaaga agtaccaaca gcagtgcttt gaagtaaaaa 59520 agtgaaatgc agtagctagccctctgggct tgggatgttg tggttaataa ctgtgccatc 59580 tgcaggaatc acagcatggatctgcattga atgtcaagct aaccaagagt ctgccacttc 59640 agaagtgtgt accgttgcacggggagcctg taaccgtgct tttcacttca ctgtctctct 59700 cactggctca aaacacaacagctgtgcctg ttggacaaca gacaatagga attcccaaag 59760 tatggacacc agaaaaagaattcttccatc aagcttaact gttttgtgat tcatttaatg 59820 acttgccctt ctgtcacttaattataaatt agatagaact gtgtcttttc tgctttgtct 59880 ttgccatttg ctgttctcgcagccacattg tattctgtgt caaataaagt ccagttggat 59940 tctagagcga atgctgtctcctgtgtatgt gaacaaaagt gaacgtaaat gaagggtccc 60000 ctcttccaag gctgaaaagccaccttttaa aagtgaaatg tgccgggcgc agtggctcac 60060 gcctgtaatc ccagcactttgggagccgag acaggtggat cacgaggtca ggagatcgag 60120 accatcctgg ctaagacggtgaaaccccgt ctctgctaaa aatacaaaaa aaattagcca 60180 ggcgtggtgg caggagcctgtaatcccaac tactcgggag gctgaggcag gagaatggca 60240 tgaacccggg aggcgaaggttgcagtgagc caagatcgca tcactgcact ccagcctggg 60300 tgacagagcg agactccatctcaaaaaaca aaaaacaaac aaacaaacaa aaaaagaaag 60360 tgaaatgtgt gtccattagagccagaacag gattgtcctg tgagtgcatc gcttagtcag 60420 aggactccaa ctgtacaagggggaatgtgg atcgctgacc ctgaaaacag cagtcagccc 60480 tgtcttcctc tggtgtgatagcagcaggca gctgaatgag gaaagaacgt gggattcaga 60540 gtcatcacac ccagctctgagtcctggttg cataggctgg ctactgatgg caatctcggg 60600 cacgttgcct gtctaccctgagcttttctc ctctataatg caggcttcta aggactacat 60660 ggcattacat ggggcacagaaagaggcttt ataaaatgca aaaccactat agaaatgtaa 60720 acattgcatg catatgacatgttgctttct cttcttagct tagaaaaata aattttaaaa 60780 aattgtaata tgtaatcttggctggcaaat gtatgaactc aatttaaaaa ctgatgtcac 60840 ctaaaattga attaacacaagagttcaagt ttcctttact accactacct catcacaact 60900 tgttaaaatg ttttgggccacagaaggaat tgccaattct agtactaatt tggtagctat 60960 agagaatctt caaccgagaaacagttttgt tgcagtggtt ctcgaaatat ggtctgagga 61020 ttcctggggg tacctgagaccatttcaggg ggttcacgaa gttctccctc caactgcaag 61080 tccatgtgaa actagtttttcttcatatac tttaaccaaa gcagtgcatc aaatgaagaa 61140 gcagacttga gaatccaactgtcttccatt gtcagacata aaagagattt gcaaaaaggt 61200 aaaacaatgc tagtcttatcactaaatttt ttcattttga aaaatagttg attttaataa 61260 aagtatgtta ataagtgaaaaaacattatc tatacacaga cacatacaca catatgtata 61320 ggcatatgag ataaagtagtgactctaaga gcactgttca tagtactttg cttcttagag 61380 acagggtctc tgtcatccaggctggagtgt cgtggtacag tcgtagctca ctatagcctc 61440 aaactcccag actcaagggatctttgcatc tcagcctcta catgcgccac tacatccagc 61500 ttagattttt gtattttttgtagacacggg gtctgtgttt tgtatatttt gtagagacgg 61560 ggtctgtgtt gccctgactggtcttgaacg cctggcctca aagaatactc ttccttcggc 61620 ctcctaaagt acaaggattacaggcaggag cagctgcact tggcctcatt gtacttttca 61680 ttcaactttt ttgtaggctcaaaatttcca caaattttta aattttgtgg gaaagtgtaa 61740 aaacaaacca gaaaatcacatgaattcccc tagaggtgtt ctcctctact ccacagccaa 61800 aatcgggaga ggcaaatttcttgttctctc cttttgggag gagggtttat gtctaagcta 61860 ccctacactg agggtgcgactcctgatgtc tcagttttct atgaaaggtt tcctactggg 61920 cttttcaccc taggatgtctttttcttaat ctcttcaacc cattatgctg atacaacaga 61980 agtttaaggt cccaggttttatcaggcact ttcagggtaa aatctagctt tggcactcgc 62040 tttccctctc aggactcaggctttcatttt atatttggtc tctatacgtt tcttgctttc 62100 ttgccagctc aataattcatttaattttaa aatttctacc ccaagttttt agttattttc 62160 actgggagga tcattcagggtatgtcatca cattacacta ctagaaacaa tgcaacagca 62220 ttttaaatgg aagttaaattgcaatcactt ttcttccaat tcttgcagag attcaaacag 62280 tggttacagc cacaagcaagagattcaaat agtggttaca accacaagca agttttgaaa 62340 aaaaaattct ttaccataatactgtaaaaa agcttgtggc cctaaggcat aaatccagca 62400 tgttcacctt tcaattacacagttgaaaat atagttgtct caacacaaaa aggtgctcta 62460 tcaaaccaat ctttctccctaatttgtgca gctcatttat tcaaattatt gtgaattcag 62520 aattctacat tctgtacaaaaaaaagtatg aagataggcc agatcatgag aaaacacctt 62580 ctcaaaagtc ataaatacgttcataacaat gtggaaatag ccctgttgac aagtgcagta 62640 catctctttt ttcatgctttttaatttgca aggacatttt tatataagaa gcaggtttct 62700 aaagccaatg tcattcatggtttccttttt agtgtagtgg gcttgggatc ctcgggatta 62760 gactactgcc tgaggatggtccactcccct ttactcaagc ctccccttaa gatttaggat 62820 agacctttcc cctggataactgagtaggtt atcatcaatt tccttagttg caagaacacc 62880 agcctcaatt atttattaaagaaaaataaa gaaaaaggta aacagacatg attctgcaaa 62940 aaacaaatct cagaattataacatcacatg aaaacccagt ccctgattat catggaataa 63000 ttattcccga gtgtccattacaggttgtag atgctggata tttttatcct gagaccttgt 63060 ttggcctgaa ccagcccttgaactctattt gtctcatctc tacattggac aatataccta 63120 gctgtacctg catatacacatgtgagatat atcgtatctc tatataaagt aacacacact 63180 ccacctgagt ttggagctcagtatttgctc tgttacaaaa gcagctgaaa tcactcattc 63240 tacttgtagt cagggcaattaaaatttact ctaagtcctg ctgtattttc cttccattta 63300 caagagggtt aggtgccccttccctaacag agtatgctct acttgttcaa agattgtcaa 63360 caataaccta cagtttccccgtttcctctt atttaagctt tgggtgataa tagattaaca 63420 aactcaatta caaatggaaacatctgaact ccactgtctt aatttattta gtgttgctgt 63480 aacagaatat ccaagactgggtagtttatt ttaaaagaaa gagagagagg tctatttggc 63540 ttatgattgt ggtggctgaaaagttcaagt ttgggcatcc acacacatgt ggtgatggcc 63600 tgaagctgct tcaactcttggtggaaagca aaaggggagc tggtgtgtgc aaagagatca 63660 catggtggga ggggaagcaagagagaaacc aaggagccag gctctttctc acaacccatc 63720 ccattctaat ccatcccacgagagtaagaa ctcgctcacc tttgtggaag ggcattaatc 63780 tattcctgag gaatccatccccaggattca cacacctctc actagggccc acctccccac 63840 accaccgcaa aggggatcaaatttcaacat gagtttgggt gggacaaacc atctccaaac 63900 catagcaccc acaaaaataaagataataat aaggaaacca ccatgtgcca ggcactgggt 63960 taagtgttta tattcatcctccaactaaga acagtactat tggcttttcc actcacctga 64020 aaacaggtcc cattagcatcttcatcttat gaatgaccac tctaaggaac agaaccatta 64080 aatggcttcc caaggtcatatagtcatgga gccagtatta aaggctgttg cactcctaac 64140 caggacacta ccccttcttgtttttaccgg ccactctcag aaatgtttca agatcatgca 64200 tcctgggaat cagtttcaggtccctgaagt cagaagccca atgctgttca ttgagagcca 64260 tgatatgtta cgcaccaccctagagctggt tcttaaccca taaggtagct atcgttccca 64320 tttcccagtg aggaaagtaagacaccactc ctttattcat ccaagagcgt aaaggacttc 64380 cccaaggcca cacagctagtaagtggctgg gccaggtttc aaacccaggt gtggctccac 64440 cgtgctgcct ctccagatcatacatagcat tatgattcct tcacgggaca gagctatatc 64500 atgatgtagc atgctggtgaccacaaccat tttggcaatg gctttctaaa aatacagaga 64560 aaaaaagcaa atactaaaaaactgagtttt tggcttcaca taatatgtga agaaggaagt 64620 acgttaaatt aattacaagaaatgcattct catggcctca attcacgtag tgtgccccag 64680 aagcaaagtt ttatgcattgattagacatt cactctccct aaggcagtgc aggcaagtag 64740 aaggacactg tgtctagggtcagaagacct cagttcgagt ccactttctg gcatttcctt 64800 gggaaggttg ttcaatctccctgaccttca gttttctaat ctgtgcagtg gaaattataa 64860 ctatagtacc tgctgaaaaaattggtgcgt tataattata actatggcac tcatctgtcc 64920 cacctcattg ttgtaaagattagatgagat caaatataag acaaagctcc ctatctgaat 64980 gagatattta aagttggttattattatgat gtgggtttct aggcagtaaa aacttaccat 65040 actcttccac agaaaaagaaaaagatgacg tgtggatata tgtttactta taataaacat 65100 gcatatatat gcatgcatgaatatgagtac aaggacatat gtattagaac attgtggtgt 65160 gcaaaagaag atgagaaaaactagtcataa tctcgcagaa gaagccctga taaagtatgc 65220 tagggcaagt ctgcaggcctctgattttag ctgcttaact attggataca cagacagtgt 65280 tgaaagattg tgttcaaggcactgcagccc tcagagaact agccccaaag cccatgaccc 65340 cacaagcaac tttagggagctgctgtggat gaagtacctg gccccactgg aaaggcacta 65400 gaaaatgagc tcttactttgcagggagtgg tcaggggaac atcaaggaaa gaagaaggaa 65460 tcatcaatgg ggcttggggctcactcagga ggagctcttc ctgataggtg ctttaaagga 65520 atcagtgctc aaaacccctggatgtgatga gctcattcat ccttgaggga ccctttgtcg 65580 cctctcacct gtccttctgaggtcctccac gcgttcccct tctctgttct tattccaaat 65640 tcctctacta catttactcaccctggattt ctgggtaaac tttattagat ttgttacatc 65700 tattttcctt tcccattagtcaatttttaa atgtggtagc caaaataatc aacatcacta 65760 gtaattatac ccatactcaaaaactcttct ggtgaagagg catggcagaa ctccacaggg 65820 aaatcctatt gtgaggattatataggtcgg aaagtcattt ttaactgaca tgcatgataa 65880 agcacatgct acataccaatggaatttaac gttcaaatgg aataataaac tatgtttgca 65940 gtatacattt ctagatccatacatgaaatc acatttccat taaacagttt tcaagtcagt 66000 gaccaattcc ctcctacttctcctgaaatt tatatgaacc tggatcattt taataccact 66060 ggaaagaaac actatacttagaaggagatg aacaacagat ttaatatttt ttcgtttgcg 66120 gtactaggag gcaagctggtaactctaatg ccctgtgtgc ctcgactcct agatctgagt 66180 agtcaataag aaagtagggaagggactacc agcaagaaaa gaagcccact aatatttcct 66240 tctccttcct tctgtgttttcttctgctta ataattgagt ctaatattaa tggacaagga 66300 ttgccagaaa aggcatgatctagaacaggg gtcagtaacc ttttctgtaa aggaccagag 66360 agtaaatatc tgagcccttcgcaggccaca tgggctctgt tacaactact caactgtgcc 66420 attgtgccac aaaaatggacacagcattgc tgtattccaa taaaacttta attatgagcc 66480 ttgaaatttg aatttcacatgattttcaca tcacaaatat taaattccaa tcatttaaaa 66540 ctataaaaat tattcttagctcactgatcc tataaaaaca agcagtgggc cagattcagc 66600 ctataggcta tagttgccagtccctggtct aaaactttta atacattaag agtattcttc 66660 tcattaaaac cactgaaattgaagtcattt tctctctttc tgcccattta cttttcccta 66720 agactttaat ttttttccccacactctcat tcccattctg atttaccagg acattacttc 66780 tcctctctac ctcacatatttgttttattg cactataaac ctcactggag aataaaacta 66840 tttgccaaag acccacctgtaaacacccag cactgcagca gccactaaca aagaggaaga 66900 cacagaacct gtggccaatcatgtggacat tggcaataat ccattccctt ctccttcttt 66960 cttcctggta gagtccacctcttataatag tctgaagaaa acagattgtt acttttccac 67020 cttcctggca aacaaaggggtatgtctgtg accaactcct ggacagtaag acctgaagag 67080 aagtctgttg gggtgcttctaggaaaggtt tttctcactg gaaaaaagaa agctacaaga 67140 gcagaatccc caccctctccttgtttccta cttttagatg atatcatgta aagagacagt 67200 gtttagagct gcagcagccattttgcaacc atgagggaaa tccaggaatt ggtccacagg 67260 cataacatca ttaattcaccaaagcacctc cacagctctc tccccctgga cttcctgttg 67320 taggcaataa taaatccccaagatgggaat tttgttgcca tttattacac cgacagactt 67380 cattcttgca aagcttacattgctagagga gtgatgtgac acataaaatg aaaaacccgt 67440 tacaaaaaaa cataaaatatcaggcaggac acagcggctc acacctgtaa tcccagcagt 67500 ttgggaggcg gaggcaggtggactgctcga gcccaggagt tcaagaccag cctaggcaat 67560 atggcaaaat cccatttcgacaaaaaaaaa tacaaaaatt ggccaggcat gtagtggaca 67620 catgtaatcc cagctacttgggaggctgag atggatcact tgaggccagg aggttgaggc 67680 tgcagtgagc cgaggtcgtgccactgcact ccagcctggg tgacagagtg agaccctgtc 67740 tccaaaaaaa aaaaaaaaaaaaaaaggaag aaaaaaccta aaataccata actcctccct 67800 ccttggcagc tctattcgacctaaatcaaa atacgttcat atctctccca cagagttact 67860 gctctatata tactttctgctttacagaca aacttcttaa aagttaatct gtccttagca 67920 gggcacagta gtgcacacctgcaatcccag ctacacagga gactgaggca ggagtatctt 67980 ttgaacccag gagtttcagaccagcctggg caacacagca agacatcccc accccacccc 68040 acctccgcca ctgtctctaaaaacccacca acttatctac ccttaccatc tccacttcct 68100 attctgtcat cctcactcctcaaatagcaa tactctgccc ttctttcacc cacattactc 68160 catcaaactg ctttcaacagagtcaccagt gacttcccag ttgataaacc caagcacagt 68220 ttttagctct tatctggcctgacctctcca cacaggtcaa aatagtcgat actatacact 68280 atacctcccc cgcaccatactttttttttt tggtttgttt gttttgagac agagtcttga 68340 tatgtcgccc aggatggaatgcagtggtgt gatctcggct cactgcaacc tccacctccc 68400 gagttcaagc gattctcctgcctcagcctc ccaagtagct gggattacag acgcccacca 68460 ccatgcccag ctaatttttgtatttttaat agagatgggg tttcaccatg ttggccaggc 68520 tggtctcaaa ctcctgacctcaagtgatcc acccatctcg gcctcccaaa gtgctgggat 68580 tacaggcgtg agctaccatgcccggcccac aaaaccatac tttccagatt ttcttttgca 68640 tccttaagta tttatttctggcttctcctt ctactaggct cttaatacta gtgttcccca 68700 gtgttcctgt acccccctccctgttttttc ttattttacc cactcttttt atatagtctc 68760 atctgtacct acagctttagtgaactaatt agaagtaatt tgtaattagc agcactgatt 68820 ttcctgttga aagttaactactaaaatccc tcgatgtgac agttctttta gttaggacaa 68880 acatgccccc aggaaatgaagtaatactat tcatttgctt gcaaaccatt ttttccccat 68940 agatgaactt cagcccaaataaaatcaaac tatccaaaat tcccagctac ttgggaggct 69000 gaggcaggat ccaaattcatgtaattttcg tgtattttgc aagtttgttt tatgccatat 69060 taattcatta cactccacatcatattttct tagcaaatac atctagacac ctggcactca 69120 gtaagggata ttcctggcacgataatcatt gttatcatta gacattgcag gaaccaccat 69180 atggatggat aaatgtgttgtttaatgaag gcaagcaatt actttaagag atcctagttg 69240 ttataaactc tctctggacttcaagtttgg tatatacata attattaaat gtatacataa 69300 ttattaattg taacatgtcctcacaattga tttctggcta tttttctaag gattttacac 69360 tttatttagt aaaatatattaaagaggtta atgataattt ttttatagag acccttagat 69420 tgaaaatgac tgatttttcacacctctttt cttgctttac taatcgtcag tgcctaattg 69480 ctaaagcaaa atatcttctaaattctgggg aggaaattct actttgggcc aggcgcggtg 69540 gctcacgcct gtaatcccagcactttggga ggccgaggcg ggctgatcgt gaggtcagga 69600 gattgagacc atcctggctaacatggtgaa accccgtctc tactaaaaat acaaaaaaaa 69660 aaaaaaaatt agccgggcatggtggtgggc acctttagtc ccagctactc gggaggctga 69720 gataggagaa tggcgtgaacccgggaggcg gaggttgcag taagccgaga tcgcgctgct 69780 gcactccagc ctgggcgacagcgagactcc atctcaaaaa aaaataatag gaaattctac 69840 tttgaattgc acactaatcaaaagactcca gcttcattta cagagacctt atctgtgcta 69900 agtagttttt ggaatagcttttttcctgtc acttaagcac cttcctttat tattaatagt 69960 aaactaaata ggagtccaaagtctgccttt cccagtgcta gagtataaat gtcatcacac 70020 ggtagaatgt aaacatcttttttttttatt atttttattt ttttggacag ggtcttgctc 70080 cgtggcccag gctggaatgcagtggcatga tcatggcttt cactgcagtc tcaacctcct 70140 gggcccaagc aattctgtcaccccagcctc ccaagttcct ggggccacag gtgggcacca 70200 ccatggctaa ctaattttttaatttttttt tttgagacag agtctcacac tgttgcctgg 70260 gctggagtgc agtggtgcaatctcggctca ctgcaacctc cacctcccgg gttcaagcga 70320 ttctcctgcc tcagcctcctgagtagctag gattacaggt gcccaccacc atgcctggct 70380 aattgtttgt atttttagtagagacggggt ttcaccatgt tggccaggct ggtctcgaac 70440 tcctgacctt gtgatccaccggcctcggcc tcccaaagtg ctgagattat aggtgtgagc 70500 tactgcgcct ggcctgattttttaattttt atttttatag agatgaggtc tcactatgtt 70560 gcccaggttg gtctccagctcctggctcaa gtgattctcc caccttggca tcctaagtgc 70620 tgagattata ggcatgagcctccatgcctg gcccctttat ccatctttta atgttaataa 70680 ttatatccat ttctgtttccttcaaaaatt caagtggaaa acctgacata aataatggta 70740 acttttttat tatacctatatcaattaaca catatgtata aaacttctac ttagttgtgc 70800 caaatatcag tgcacagtatgggtgatttc ccccacttgc gaagaatggt gctatagtca 70860 ccgcacagga gcaggatagatccccactct caaaattggg tttggatatc aaaatcaata 70920 atgccatcac acatgcagcaaaggggtttg aaaggtttat tgctcacata atacgttttt 70980 ttgggggaga gtaagatgggcttccaagct ggtctgaaaa ctagagagaa caagaaagga 71040 gaatggcttt gggattcttattgtggttag gagtggggct gtggtgaggg ttcctgtaca 71100 tgggctaggg cttactgttggaactttgtg ctagtgccaa ggaaggagca cctaggcatt 71160 cttatcagtt tgctcagatgtggggcagag aggaagggaa gtggtggggc ttgagaactg 71220 tctgcagtca aacatcaaaaattaagtcat actctgtatt acaacaatta gaaaagaaat 71280 aaaatgtagt atatcagttctctattgcta caatattgtt acataacaaa caacccccaa 71340 acctcaatgg tatacaaaaataaatgtgtc ctgctcacac atctggagtc aactaaacag 71400 ctctgctgat cttgcctgaactcattcatc tgtcagcttg atcttggctg gctttgccag 71460 gggtgggcta taccctgctctatgtctctc atcctccagt aggccagcac aggcatgcta 71520 tcatggcaaa ggcaaaggtgcaggagtaaa ccagcccaat aacacaagca ctttttaagc 71580 ctctgcttgt ggcacttttgccaaaatcct gttggccaag catgttacgt ggccaagcca 71640 tgagatgtgt acacagaaaaataaaagatg tgtacacaga agagcaaaga attggggcca 71700 tttttgtaac ctactgcacctattaatgga ttactaatat gtcacaactc ttatcctaga 71760 ttacagtatt aagagaaggaaagtcaattc aatgatatat ttactactaa atgttaggta 71820 ctgtacttgg aattagagttacaaaactaa atgagataat cctaaggagc tcacagttgg 71880 atactggaaa aaaaatcaaacaaattaata cattattaaa ataaatgaaa taaaatccaa 71940 tgtaagtaca tgaatagatattttaataag aagtggcatg gaaatgaaaa taaactcttt 72000 tgtctccttt atcatatatttcatagctaa ttaaattaat tttattataa tcaaatttac 72060 tgtagacctg aggacataaagtttactgag actccctagc gaatggctgc ataaaaatgc 72120 aagccctcta aaagcaaaaccagattgtaa attctgttag gctaacagaa agtcatttga 72180 ttcttttgtt tttccgttacacttagtagt caaccaaaat ttattgtgtg cctatatgtg 72240 tgtattagtc agagttctctagagggacag aactaacagg atagatagga aggggagttt 72300 attaaggagt attgacgcacgatcacaaga tgaagtccca caataggccg tctgcaagct 72360 gaggagcaag gaagccagactgagtcccaa cacctcaagt agggaagctg acagtgcaac 72420 ctttagtctg tggctgaaggcccaacagcc cctggcaaat cactaatgta ggtccaagag 72480 tccaaaagct gaagaacttggagtctaatg tttgcgggca ggaagcatcc agcacagaaa 72540 aagatcaagg ccagaagacgcagcaagtct gctcttccgt cttctcctgc ctgctttatt 72600 ctagccatgc tggcagctgattagatggtg cctacccaga ttgagggtgg gtccgccttt 72660 cccagtccac tgactcaggtgttaatctcc tttggcagca ccctcacaga cacacacagg 72720 agccatactt tacatccttcaatccagtca agttgacact caacattaac catcatgatg 72780 tgcaagctat tttgctgggaatttgctagg atcgaaaatt cctagttttt ttgttgtttg 72840 tttgttttgg ttttgaatctttaatgagaa aaaatactat tgtgctcaag aacaccgtgc 72900 gtctggtgtg tcattgggtcaagggttggg gacacacatg gcagaattca gaaacagggt 72960 aacatcaaca aaaatacacacggtcataaa aagaaaaact ggcacctggc atgatcaact 73020 ctgtttcctc atcattcagcagcatgttgg ggatcctgcg gctgatgggg aacacacgtc 73080 cagactctgg gcactgcagggtgccctcca acacatctac ctccagcagc acgtggtgca 73140 ttgtcctcac catggagattcctagcaaat actctaggta ttttaaatag aagaaattta 73200 atacagggaa atgattacatatatagtaga agaactgaga tgtcaaacag aagagtgagg 73260 caatccacat tttagcaactgcagtaagtg acctccaatg tcatggctgg agggtgcaaa 73320 tggtgagaag gcagaagctagaatcatggt ggaccccacc tgcaagagtc acaaagaaga 73380 tgcatccatt caggtcacagagaagtaaat actctagtgt ctaccttcta ccttcttatt 73440 gctctctagt ctcccattttatctagactg ctgacaagca tgcagtggaa ggtgagcacc 73500 agatctgaga ggaaacaggcagtgtctggt ggcacactac acaagaactc tcacaggcat 73560 ccactaggct tgcttggtgactcatttaag gagtaaccct attttatgta tcagagtagt 73620 ctatggtgtg atatagcatattgctgcttt tgatgtttgg tttcatttgt tccctgtagg 73680 cacctaatca ctgttttttaaattccccag gagattctgg tatacccaat cagctgagaa 73740 ccactgaata gtggctttcaaaattttgag catccaaata ttgtcttcac agaaaatctt 73800 aatctctgaa gtctactgtaaaaacataga aaggcaaggc tgttctgctt gaagcgtagc 73860 ttgggagcct agattcctctcaaacccagg ccactcctgg gaacatggtc tggagatttg 73920 aatgttccca aatctagtttatctcattag tcgatattta tgcacatcct ctgtgtctct 73980 cagtgttctc tcatgtgtagacatatgccc cattccaaag agtgactgta gtgtaatgtg 74040 gcattaaagg aaccctggatagacctgtat tggaccttac atattagaga gctctgcaga 74100 tagctcacaa atctccaagtcgctagctgt aaattgagtg ataattggtg tcaggttatt 74160 ttagcacagt ttggggataaaatggtgcat taatctccca gtttgtggct tattaaactt 74220 tgaaatggaa ttatttgagacataaaagag atagcaatac tttgctggat gttctccatt 74280 agtcgagatt gttacagaggagaaattttt gccactgttt atcaaatatt ttttcctcta 74340 gttaatccat atcattccgggagctttgcc caggaaaagg acgaattact tgcgttcctc 74400 cgatctcttt caagttctactcccgtacct ttaaactgtc acacggaatc tttggtgaca 74460 gaggaataga ggtgggcaagcagggcgttt ggaaaccata ctgaaaacga attatccata 74520 aagcacagat tcaccagcctagaaatggct ctgtctggat ggattccttg agatgtgttt 74580 tatctccatt aaaaacacaacaactttcaa actttcactg tgtctcaaaa tgatagagaa 74640 aaaaattcag cctttcaagtattcaagaaa agcttgaaga aataaaagaa actcgctgct 74700 ctctgggcca ctttaggaggcagagcgttc ctggagaact tcaggattca ttcgcaaggg 74760 aacccaaggt gggcattcaaagccgatcca aaggatattc ttctttttag aaaacaaaag 74820 gagcgcaagg agtaggggactgaaggtgcc caaagctagt gggtcagtgt cactttcttc 74880 ctccctttcg cccctttcctcccgctcctt tctcccctgt ggtctctgct cagcacccct 74940 ttctctccag ccccgactgcccatcttcgc ccctaccctc gcgcgtcggg gccgtgtcta 75000 cacccacccc aggacctggtccgtccaggc tccttcccat cctcacaccc gcgcctttct 75060 ccctgcggcc ccggctcgctgctccagctg cccagctctt cccaggacag gggatcccgc 75120 caagggggcc gcccccgccccggccctccc ccgcccggcc gcaccataaa gcgcccggcc 75180 gctgccgcgg agcccagcagccagctcccc agcaccgcgc ggcggggacg cgagcgcgcc 75240 cccgacggca gcccggacgccgagcacggg tcacctgcgg cgccggcccg ggcgccgacc 75300 gaggttcaac gcacggcccggggaccccca ggcggggcca acgccgccgt cgcccccggc 75360 ctcgcgggga gcaggaagagccaacatgct ggccccgcgc ggagccgccg tcctcctgct 75420 gcacctggtc ctgcagcggtggctagcggc aggcgcccag gccacccccc agggtaagtg 75480 ggttcgggtc gggcctgggagcgccgggca ccgggtgccc catctgctga gtgagtggag 75540 ggacttgctc ggccctgtgctcctgtggcc ttgctcagcc cctctctgtc cctcccgggc 75600 ccaatcaaag catattgtgattggaggtag tgattagtct agaattttcc gaaattcgat 75660 tatccaatga tgagaacaattgcctaaata gttgggggat gcaaaattgt ttcagactat 75720 gcaaagatgt ggggtggggttgccttcaaa acttgctaca cggtcctaga cctcttaaca 75780 tgttaggctc tggtgtagggtgaattccgg gtcctgcacc ctgtgccggt tccctccagg 75840 cagccttgct ccctaaatccttcctcaggc ggagcgatgt atgcccagag tctgctcttg 75900 acatccctga gaaagacgcagaattactct acttgccctg ccacctgcat tttctgctct 75960 gactttgctt gaacgacaaaaagaaagttt atttcaagga ttggcttgat gtcttaattt 76020 ctgaggtaaa taggaagggctgagaaactg gtctttttca atgaaatcag ttctttgcag 76080 caagatggat gcagctggaggccattttcc taagtggatt aaggcaggaa cagaaaagca 76140 aataccatgt tctcacaagcgggagctaag cagtggatag atatggacat aaagatggaa 76200 acagtagaca ctggggactccaaaagtagg ggggaaggtt gggaaaacta ccaattgggt 76260 actacgttca ctgggtgatgtgttcactag aagctcaaat cccaccttat gcaatgcacc 76320 catgtaacaa acttgcacatgtaccctctg aatctataat ttaaaaagaa aaaagaaaga 76380 aattgatctt tttctatgaaacggtaaaaa ttcaacaaat aatgtgaaaa gatacttgga 76440 caatcctcaa accccttggtgaaatggggt gaaatagcca ttgggtagaa agcagttaaa 76500 cagaattttt tgtaaagaccaaaaggaaga catgatttaa gcaaagaaag atcttagggc 76560 tataactggc cctaactggtcaggcgtgag cttcatctgt ttgaacttgg tgcctgggtt 76620 gatttattac tgactgtggaagtaaaactg gaagagacgc acaacttgaa gtaaaagttg 76680 tttttcctct tgtagccagagcaaagactg cctatgaggg cgttctcaac ttccctgcct 76740 ctcgctgctg gaatcacccatttaaactgc atttcgccaa ataactcatt ttcattccag 76800 accagacaac cagaactgtggtttcagggg ttagagtact gctaactttt agtttgtgtt 76860 gaagtatttg aaatgctgtagtccgtgccc catacttcta ggaaggtaaa aaaggaagct 76920 tttaaacact tcaaaagtcttattgtgatg atatttggca agttcaaatt tataggtata 76980 atttttttgt gttatatacgttcttgactt ttgaacatac agctttatgg tgtgccttag 77040 gtatgtctca gacatacctgaccattttat ggagatttga actcggccta tacaacctaa 77100 attatctggg taatttagttgctgtagatg atctgattaa gttaagtttc tgatagattc 77160 ttttattgca ttatctagtctgcaattatc aggcttaaca ggataatttg attatctcct 77220 tcttcccctg agcttcattgttggtcttaa attagtatat attcaattta cataccatgg 77280 tagctgcgct cattggtagcagtaatttca cctctaacag tagagggttg tgctttgatc 77340 ctggcactta aacgtaataacattaataga aaatggtagt gtctcatttc cttttcaaaa 77400 cactctctat gttcattactaactaatccc atctctgccg cttgagagag atgcaaatga 77460 atcagacgaa ccatagattgaaataccgca agtttagctt gagttacatc atgaacctga 77520 tcatagtcaa aggaagaatcaagagtacta agaattttgt tttgaatgta gttcccatac 77580 tttaaataca tatctcttttctgaagatgt tttgtcctga atggaacttt aaaacatatt 77640 gccttatttc cctttgccctcattagtgga aatgtaaagt ttccaattct cttgaaaatt 77700 aaaaaatcca atttcccttttgaaatgcag taggcaacag tgtttatggt ttagggatat 77760 aggcactaag aggagcacagtaaatgttct gctttccaac acatctcatg ttaatttcat 77820 tattgtgtaa ggacccctggtacatgtgaa aaatacagta tagatccagt ttgaagacaa 77880 attgtctttt aaaaggggtaaatattcttg agtaactagg tttttgcctg tttgctttag 77940 aacttttatt cattcttattacaaaagcaa tacctgccca ttgtagaaaa gattaaagtt 78000 agataagcaa aaaataaaataaaaagaaaa gaggaaattt aaaacttata tttatgcaac 78060 ccggggatag cttctattaacattaacatg ttgttgtatt gtctttcatt tcaaatattt 78120 ttctatgcaa aaatatatttcaaatggaat tacgttatac atatagtttt agaatccttt 78180 tttcttaatg tatcatgaacatcttttcac atctgtaagt attcagctac attttaaaaa 78240 taggtagtat tctgctttttggaagaatca aaatgtattt agccagggtt gtaatataga 78300 acccttaagt tgttttcggttttgatattg taaatattaa tttattgaac aagtgcaggt 78360 gtcacaggat ggtttgggatgagtttaacc atcccctcaa tctcagccaa gcccttctct 78420 ttttttttgc taaggacagttaatgttttc atagcaaaat gctatgccaa cagagctcag 78480 gtttacctct cttccaattagattttcacc taattctttc ctaaatagaa attctagaat 78540 taccactagt gtctaatattcttgtaccta aatcatttac catgttatcc attatttccc 78600 taggataaat tcccataagcagaattgcta gatcaaggag cacatatgtt ttagggcctt 78660 tgatactgtt gtttaaactgtcttcaaaat gtttatacta gtttaaatgt tacacagtgg 78720 tacagagagt gcccatttccctgaaccctc acaaacagtt gagtactgtt gtatttacta 78780 attttccaat tcgatggtgaaaacaatacg ttattgttta agttagcaac tcctgcatca 78840 ttaggtaaat acatgaacatttatttatat acttaatggt cattgtaatt ctttttatgt 78900 aaattgcctt ttcatattccttgcccattt ttctattaag atgcttatca ttttattgat 78960 ttgtaagaac tctttatcaattgtcaatat tacccattta ataaccagat tattatatcc 79020 aagagacagc tgtaggtgctgaagtttctg cagcctatgg aattgttcaa atgatatttg 79080 cgtctgctaa tagatatactaaataacttc cactgagtat tacccaaatt tggcctttgc 79140 cttttaaatg actctgggcatactctctca tagctttcat aaatattaca aagtttgtga 79200 gagaaagtac tgcttgcttgatcaaaagca acttatacaa atacaaatgc agattctgat 79260 ttttgtagag caattctatcagtcaagaga cagaaaccac atcattttgt tttgttttgt 79320 ttccacattg ggatttttttaaacagagaa atgttggaga attgtaaagg cacaaaggga 79380 acacttaggt ctcacaaaggtggcaaatca ctttctacca gcacatctac caccatagcc 79440 atgttttctg ctgcatttcctgtcatttgt cactgaccag gaaaacacaa ttggctctgg 79500 tcctggaggt gcctatgggactccgtgaca gaaattcact gcttggggac atcagtaaag 79560 caatggcttg gatgctttcaccagtccccc ttgtacttta agcctttagt gcttttcctg 79620 gtgggcattt tcagagctacttgttcagcc aaacccaatt atgtgtttgc aaattcagtc 79680 ctctctcttg agggctacacccagtgatga atattgtgct gtatttcttc tggctcaaat 79740 gtccagccac tataaaagtgacagggaaga actggtgagt ttggcaatac ggaaaaggga 79800 ttacaagatt agtttacattactttgtagc ttactgtcaa attgcacccc ccccaaacaa 79860 agaaaccctg ttttccccaaaattgaatct ttcacttata ccccttcttc tcccttcttc 79920 ccagtctttg accttctcccatcttccagt cagaggctaa acccaggcgc tctgctgcca 79980 gtcctgacag accccgccctgaatgatctc tatgtgattt ccaccttcaa gctgcagact 80040 aaaagttcag ccaccatcttcggtctttac tcttcaactg acaacagtaa atattttgaa 80100 tttactgtga tgggacgcttaaacaaaggt aagcaaattt gctgaaatta ttttcttaaa 80160 aatctccttt ttctattccaggattagggg tatactgtct ttgagatggt ttccttgtac 80220 aattatattc tcactcactagtcatttcag tttttaatac tgaattcttc ttactgtaca 80280 tttacctata ttggcgggggggtggggggc ggttcaggaa gaaagtgaca ttgggagaaa 80340 aaaagaattg ctgtgaccaagatgcttttt gacattgtct catcctgatg ttattctgaa 80400 tccagttctg gaacagagaggacaagggac aattcagcca ttatcttagg cagctcaggc 80460 tgctataaca aaataccatagactttgtgg cttaaacaat agacatttac atctcacagt 80520 tctagacagt ggtaagtctaagatcatggt gccatctcag tccattttgt gttgctataa 80580 aagactaccg gagactgggtaatttataaa gaaaagagat ttatttagct cacagtcctg 80640 caggctggaa gttcaagggcatggccctgg cttctgacaa ggactttctt gtgtcacaac 80700 gtggtggaga aggtcaaagagggagcagac acatgggaag aggggaaaac ctgaggggca 80760 ccgtagcttt ataataacccactctctcaa gaactaattc attcccttga gaactaatcc 80820 attttcacaa gagctgaaactcactgtctc gagaacagca ccaagccatt catgagagat 80880 ccaatcccat aattcatatgcctcccacaa ggccctacct cccaacaccg ccacattggg 80940 gaccaaattt cagcacgagttttgacaggg acagacatac catatccaaa ccataccacc 81000 cgcagatttg gctccttgtaagggcccagt tcctggattg tagatggcca tctttttatt 81060 gtgtcctcac atgatgaagacagacagcaa gctctcttgt gtctcttctt ataaagtcac 81120 tattcccatc atgagggccacaccctcagg acctcatcta aacctaatca tctcccaaaa 81180 gccccacctc caaataccatcacactggag attaggagtt cagtatatga atttgatggg 81240 gcgggaggaa ggggacagatacaaacattc agcccatgac agccacccca tactattctt 81300 atgttccctt tgaagccagtgctcagtaga cctagctcct aactccttaa gcacctacct 81360 ggccgtgatg gaaagaatatacctcaacca gtagaacagg atgaataggg ctcttttctc 81420 cctgtgcgtg ccaatttacacaggcctgtc actggtttga tccctctgct gtcatttgtt 81480 ttgttgccgt agtgagttactataagcact gagaagtgag tcccaaagaa aatggtcact 81540 atttttcatt gccttctcctccactccttc cccgggggac aacacctgtt cctgtttatt 81600 aaggaatgtg acagatgcgtgttcctgctg cttcatgcat ttaataaaat aggagggctg 81660 gttcttctgg ctgggttttatgtttatttc agtttgcttt acaaagtgga cacttcctag 81720 tgcttcaaac tctctcttagattatatttg tgattgtaca cttaaatctg caggcattag 81780 gaaatgaaag aagtttctaattcttaactt tcataggact ctaaagttaa aagtataaat 81840 gaatgggact ttaaccccttcttttctgtc agattccaac atgcttcttg cctgcttcca 81900 cgctactccc aagtctgctttctctgtatg tgcctcatag cctgtcaggg gccttgagga 81960 ggtgtcagat aataaagtgagtctgaggac atggccagca gatgggtcaa ccgttcctgt 82020 ccccctttcc ccatcttcagccctaatgag gggggattct gcccatgtct ctgccttgaa 82080 tcaaacagcc ccattttgcctagcatctgc tgaaggaact gctcatcttc tgacaaccca 82140 gctactcagt ttgtgatcgtaaaaatcacc gttcttcatc tgtggatcag cagcttctcc 82200 taacctgtct atttcagaggcattgcaaag atttttcttt cggttcatcc agcttaatgg 82260 ccagaagttc ctctgcagagtaaaaaactg ctccaagctt ttgccctcac tgcctctcac 82320 cctctatttc taaattggccagtatgtgtg agcccattgt ggaaagtctg ttgctgggaa 82380 acacaaggaa gcaacttcccctgaacggat cctttcagtt tgtatagaca agagtaacac 82440 tggatttaat gggcagtaggtagagggtaa ggggatcgtt agtatgtttc ctctgaagaa 82500 tcaccagtaa tatgagtcagctgtaaatac cagttgatca aatattttag gggctgtaat 82560 cccagcactt tgggaggctgaggcaggcag atcacctgag gtcaggagtt cgagaacagc 82620 atggccaaca tggagaaaccccatctctac taaaaataca aaaattagcc aggcatggca 82680 gtatgtgcct atagtcacagctactcggga ggctgaggca ggagaatcac ttgaacccag 82740 gaggcagagg ttgcagtgagctgagattgt gccgctgcac tccacacttc agcctgggtg 82800 acagagcgag actctgtctcaaaagaaaaa aaaaattaga ggaaaagatg aaagggttga 82860 gatgaagaac tgaaagatgaacatctccct accctccaac ctattaggaa agataaattg 82920 ttcttagaaa ctgtgggggcagaaggagtc ctggaagaat ctagaaatga gaattctcat 82980 aaggcaaaga gggatttatgctgttattag agcttgaaga gttgaagaat gacacagaga 83040 agttgtttca ctggagcagtcggaaaaaat acgaagattt gccagcactg gtgaatataa 83100 aagtttcagg aaagagaagtccattctgac tggaacaata aaaagaagtc actgggtcca 83160 tcagatccaa ggaaaagacagagcctgtgg ttgagactcc ccactgcagt gttcagtggt 83220 ggccatgctg acttgacataattctcccca caaccctcca caaattatct acctggacat 83280 ggtaggctcc agacagcattcatctggcca aggttcagct gatctcctct tttgctgatt 83340 tgcgtggaaa acggtgaaacagaagaaatc caagtggatc tctactgact taaaacacct 83400 gagtaggaaa taaaacacacccttccctcc attaaagtcc aggactggga gatgaaggag 83460 ggtgactaca cagcatcaaagagcagaatt tggtcatctg ggcctcatat gacctcctga 83520 aacaaattaa aatgtagctaatgaagactg caaagaaaga gtttgccact gagctctctg 83580 cttctactct caccctctccaagtcagcct accccaggcc acctcattac tgttcacaaa 83640 cataatttgg atcaagatacatctgacctc tgagacctct agtggccccc tttccaaatt 83700 gaataatgta cccatgtctcagcaaggcat tcggggcttc cactctgcat ggccccaagc 83760 agcctttcca gtttatccctcattccagcc tccatgcagc ctgagcttca ccaagatagg 83820 ctattgccat tccttggggccgcacatgct gcctctcctc catccttttg ttcctgctgt 83880 ctgctccctc gacacatatcctgcatcacc tcttgacacc caaatcctac acattcaaga 83940 cccatctcca gctccttctgatattctttc taatatgact ttgtacctct taaggaagac 84000 attcatctta gctcaccttgtacttgaatt acttctaggt cttctctcca tttggttggg 84060 agctctcttg agacagggactatacagccc ttatctttgc atctgctgca gcacctagca 84120 aggtgccttg tacatagaagtagttcatgg atagattgaa aagaataaat ggaggctgag 84180 aagagtagac ttgtttgtgaaaaatgacat acaccattgt gaaattctgg gattccacaa 84240 tatctgacac atagtaggtaataaaaaaaa aattatttat ttatttatga gacagagtct 84300 tactctgtca cccaggctggagtgcagtcg ctagatatcg gctcactgca gcctctgcct 84360 cctaggttcg actgactctcctgcctcagc ctcccgagta gctggaacta catgtgcgca 84420 ccactacgcc cagctaatttttctattttt ttagtagaga tggggttttg ccatattggc 84480 caggctggtc tcgaactcctggcctcaagt gatcctcctg tctcggcctc ctaaattgct 84540 gggcttacag gtctgagccaccacactcag ccaaaaaaag attttttttt ttaatgaata 84600 aatattgaag ggcttttggaataggatgta agaggataca ggtagtattt ctggtagaag 84660 tgatctgcca ggttatgagtaatacagcct ttaaatcttg atagaaataa gcatcctgca 84720 gaaaccacct ggctggatagaagatatggc tgatgctctt ctagtatctg gcgtaaagac 84780 aactttccag tttgtatcctgagtttgagt cacaaactgc atcaccatga atacatctct 84840 gaatctctct tagcccttgttgattgccta taaaatgggg ataataatag ctacctcaca 84900 ggctggttgc aagaactgaattatataagg tataataact ttcttcagac tatgaggctt 84960 aataaaattt tcatactcttaaacagtatt agagacagat ttccactatt taggcaactg 85020 ttttctaatg atgatggtttggtgaactct tggcttgcct tgatgacaat ttcacctttc 85080 agaaaaagaa acacaagaactgcttcttta gtcttagtat gattgtctca gaacaacttg 85140 gtgctgtgaa gtgaaggaaacccaaagtgt ttgctgtaat gaccagagag tgtcagtgaa 85200 actccttagc ctaaggataggacatgtcaa atagttcaga gtagtggttc tgtagcttgc 85260 aagctaaaga gaaatatagattaagagaga tggtagagct taaaaaataa aattatagac 85320 acaataataa gtggaaaaaatgaggggcct caaacattaa taataatatt tatggagtct 85380 tttttttttt tttttgagacggagttttgc tcttgttgcc caggttggag tgcaatggcg 85440 cgatcttggc tcactgcaacctccgcctcc cgggttcaag tgattctcct gcctcagcct 85500 cccaattagc tgggattataggcatgcgcc accagaccca gctaattttt gtatttttag 85560 tagagacagg gtttctccatgttggtcagg ctgatctcaa actcgcgacc tcagatgatc 85620 tgcctgcttc ggcctcccaaagtgctggga ttacagccgt gagcaaccat gcccggccct 85680 atggagtctt tttaatgtaccaggactgtg ttaaaagctc aaagcctatt tcccctttta 85740 actttcacaa tattcctatgacatgccctt gaattagctt aattttaaag tggtcatgtg 85800 cagaagatgg aaagaattttaaaatttgac acaaaactat aataattagg acaaaaaagc 85860 taaaacctaa ttgagtcaaagttggtgatt cacacataag ataataaaat ggatgttttt 85920 agccatgttc agaataagatatggaagata tggaagaaat aagcctacct cctggaatag 85980 gtaatgggag attaacaggtgatagaggta agtcatagat ggctcttttg tttctgtctt 86040 acccatcaag gggagtgttcttcaaattgt caagaacagt taaaagaaaa caaaaatcca 86100 agctagatga ggagatagcagaaaaacgcc cactctcttt agtgagatta actattcagg 86160 agcagatgaa ttacacattgggatatttag tgaagaaagt catagtggag ccactgttag 86220 ttcatttaga aatcatggagaacagaaagg tcctaaaaga caggacaggc aaatgtttcc 86280 atttttataa agaagggaagggtggattct gaaaaccaca ggctactgaa atttaggaca 86340 gagctccaga gggttatatgattattcatg tattttggga ttgcccagaa aagaaatctg 86400 ggattttttc taagacaaagtggatttact aagaataaat catgccaaac tttttttttt 86460 cagtaaagtt tcaaagctgatagattaaaa aaacatgctg tgagcataat gtatctgact 86520 tcagaaaaga attggatgaagtcgcttatt atacctttct aaataatata aaaaagaacg 86580 agctaggtaa tagtaccgctaggggttgct aagtcactgt atagcaaatt atctagtggt 86640 ttttgacagg gctctgtcttgggtattgaa ctgtccaaca tgtcagtgac ttgagcaaag 86700 acagcatttt atcgtgtttgcaattcaaag ctagaaaaca gttgaacatg ttggatacta 86760 tcattcatat ttacaaagtcctaccagata atacaatggg ccaaaatcaa tcagatgaaa 86820 tttaacaaga ataaatgtaaagctatcaac taggagtgaa aagcaaattg cagaagttca 86880 ggatgtgaga catctgtcctaagagttctc taatcaacaa caaatcctaa tcagtgttga 86940 ggatagtgcc tgacacataatggatactca aaaatatttg ccgaatgaat gaatgaacag 87000 atactgtgat gcaactgtttataaaaagct ccttacattt taggtttcat tagtgcatac 87060 atatggccag gttaaagaagctaagagtcc cactgtactc ttaattgtcc aggccacatc 87120 taatactgtg tctagtcctggacacatttc acaaggacac tgtcaagtca aagattacct 87180 tgaaaagagt caccaaaagagcacctatct ggaccccgtt tttttgtttt gtttggtttt 87240 gttttttgtt ttttgttttctgttttttgt ttttaccaca tttaagtaga aaagttaaag 87300 gaactagaga aatgaagaagccagacagaa gacatgaaaa atatgaaaat tgccttacaa 87360 tatttgaaaa aagggctaggtgccgtggct catgcctgta atcccagcac tttgggagtc 87420 tgaggcagga gtatcacttgagcccacaca ttcaagacca gtctgggtaa aaaattaaga 87480 attttttttt tttttttttgagacagagtc tcaccctgtt gcccaggctg gagtgcaatg 87540 gcgcgtcctc agctcactgcaacttccacc tcccaggttc aagcgattct cctgcctcag 87600 cctcctgagt agctggggttacaggcaggc gccaccacac ccgcctaatt ttttttgttg 87660 ttgtcgttat ccttagtagagatggggttt caccatgttg gccaggctgg tctcaaactc 87720 ctgaccacgt gatccacccacctcggcctc ccaaagtgct gggattacag gcatgagcca 87780 ccgcacccag ccaaaaatacattttttaaa aaattagcct ggctcgctgg tacatgcctg 87840 tagttccagc tactcaggaggctgtggcag gaggatcact tgagcccagg agtctgaggc 87900 agcagtgagc cgtgatcatgccactgcact ccagcctggg caacagggca agctcttgtt 87960 tcaaaaaaat aaaaataattaaaaataatt tttaaataat atttgaaaaa tgtcatttgg 88020 aatgaagatc agactaaatctctgtctagc tctgaagagt aaaattagga tccattggta 88080 gaagacagag gaaacagatattggctctgt attaggaaaa tcttggagag tgatccaaaa 88140 gtgaaatgga cttctctgcaaagtaatgag ttccttgtta ctggaaatgt gctggtagag 88200 gctgcacttt tattgaaggaatttgtgaag tgagatatta agctggatga cttataatgc 88260 taatattttg tcattgtaccaaggctagag aagtttcata ggcacttaag aaattgcttt 88320 gcacctcttt cctgtaacatgaccttgggc ttctgtgcac actcactgct ttgttgtcta 88380 ctagatagtt gcatactatgatgaatctca tactttttca aaacacaaag tcctctgcag 88440 atgtttagca taattgctgtgtttcctttc actagataga tgtgtgtgtg tgtgtgtgtg 88500 tgtgtgtgtg tgttactctgtatactttac atttattcaa attgccataa gaaaactttg 88560 aaacagactg catttatcacagctaggagt cacccagagt actttcagaa aaagcaattt 88620 gctgatttac cttttgtatgtatttccaaa gcactaaagt tacaaagacc tagtgtatag 88680 ggcatgaaga tgaataagcaaaggcaaaca agaatatcca tcacaagaga cattaatgtc 88740 aaggaggcaa tttttaaatgtacaattatt taaaaagaag aggaaagatt gacaatgatg 88800 gcttccatca tatgtttaaaagtgccaaag agcaaagcca tttgccaaac aaagcaaaga 88860 gctccagact acctttgtgagtttgctgcc tgcaggccag gccactctgg actcctttgc 88920 ccaagcagac aaaatattatcatttccagt tctttaaagc tggcaccctg cttcataact 88980 ttgacattca acatatcataatatttttga ctaccaggtc cacttttcaa aaagcatctt 89040 ttgaaaattc tttctgaagatagcaatatt tcaaatcttc attgcctggg cttgcttttt 89100 catctcctgc cagtcactgaaatcgtattt aatcagactg ggctatgtgg ttcctgtgtc 89160 cggctggtcc caagcatggaaatgtagctc ttggacagtg ccataaaatc aggcaatcct 89220 gagtagctgg ggttacaggcaggcgccacc acgcccacct aatttttttt gttattgttg 89280 ttatccttag tagagatggggtttcaccat gttggccagg ctggtctcaa actcctgacc 89340 aaattgtcaa ttacacaaagatgccaagtt tagagggaaa ggatttatat aaccaacaaa 89400 gtgttccaca ttttaaaaaagacataattg tgagtaataa aagtcagagt ggacacactc 89460 tattttatct attttttttcacttcccata agtagttgta gtggtgccct cagatactat 89520 gggatgtggg aaaatcagtaagatatagac ttggcagcat gaagggactt aaggtggtgg 89580 gaagattagg gccagtcctgggataaggag ttgaaacatt atttagaatc cactagggag 89640 ttattgaagg atttcatttgcttgcttgct ttgaggaggc aataacgatc agaacagtcc 89700 tataagaagt tcaatctctcagtccaaaaa gtccgtgcag aaagagacct aaagtaggaa 89760 ggggagtcag gaatctaacagagcagccca gaggtggagc agagtcagag acaccgtttg 89820 agatccgagc acccactgactgacagcctt cagttccaat ccaatctgca ctgcattctg 89880 ggtactaacc ctgcacaataaccagctgca attgccaaga aaacctgtgc agctggtggt 89940 gagtgaaaac caaggatgtgaactcaatgt gctgcagctg aagaaacaga gttgtacctg 90000 gaaccgccca gtgggttcaccttgcccact gcctacacag aacccattta tcaagacagg 90060 ggaattgcaa tggagaaagggtaattcacg tagagctggc tgtgtgggag accagagttt 90120 tattattact caaatcagtctccccaagca ttcagggatc agagttttta aagataattt 90180 tgcaggtagg ggcttgggaagtggggagtg ctgattggtc aggctggaaa tgaaatcata 90240 gtgggtcaaa gttaggttttcttaacgtct tctgttcctg ggtgtgatgg cagaaatgat 90300 tgggccacat tactggtctgggtggtgtca gctgatacat ccagtgcagg gtctgcaaaa 90360 tatctcaagc actgatgttaggctttacaa tagtgatgtt cccccaattt ggaggtattt 90420 ggggagcaat ttggggattgctcagactct tggagccaga ggctgcatga cctctaaact 90480 gtaatttcta atcctgtagctaatttatta gtcctgcaaa ggcagactgg tccccaggca 90540 agaagagggt cttcgggaaagggctgttat caattttgct tcagagtcaa agcatctact 90600 gaattccttc ccaaagttagttcggcctgt gcccgggaat gaacaaggaa agcttaagtg 90660 ttagaagcca gatagagtcggttaggtctg atttctctca ctgtcataat ttcctgagtt 90720 ataatttggc aaagacagtttcatacccag tgactgtttt cctgagtgct ttccagggtg 90780 gtggccaggc agaggtgtggtcgagtcggg aggtgggatg gttccagcct caggggcctg 90840 gcttcttctt ccttcctggagggacaaaat ggtcatcttc ctcctgctgt agtgttttcc 90900 cctgcgtttt gttccttctgctatgtgtgg atgtgttggg ataagatagg gcaagatgag 90960 caccgggaaa gggggctggcaagaggaagg gaaccctgtc tgttctcttc cccttcccag 91020 tgatgctgcc tctggagaaggtgaggaaaa ctcgcaccca acaggctgca ggctcctggg 91080 gccagagctt tctgctttttccagactcca tatgactgat gtgaaaacgt tgagtttcct 91140 gagcctgctt gccctctgggtagtctaact gctctgataa taaaataagt gtagaggcac 91200 tgaagtaaag aatttgcaataaaaaagaaa atgaaaaatg gaagccataa gagaaatgag 91260 tattggatca agtgtgaagaatgggaaatg tcaaggcact tgataatttt tctgctaaca 91320 aacagacatt gtgaaggtggaagtttccat ggttttcctt ccctgcgttt tcttttgcta 91380 ttgtcatgag atctaggggcatgagggatt tgtcatttga gtgttacatt gcaacaaaaa 91440 gcaaggcaga agtacagaatccacttaaat gagagtatga acacaatagg aggttttgct 91500 acctggaatt agatgttgaattgctgctct ctaaacttta ttttctatat aaaaagcttt 91560 aaatacaatc atttaattattcatttaaag tttatattaa caacatttag tgtgcacctg 91620 ctatatgtaa agtatcccactaggtactat tcatcaaatt aaagccagct atttagttat 91680 gaaaataaac catggcccacataccctgtg aagccctgtt aaaagcaggc aacatgccca 91740 agaaaatatg tttcctttgaatttcacaga aatcttagtt tgaggaagaa aaatcaagag 91800 tttttgttat aagccactatgtgtatgctc tgaaacagtt aatgtagaga acagaggtag 91860 tggccataac tgaaataaggagatagtgag tgagtgtgtg tgtgacctaa ttcaagtttg 91920 gtggtccaag tggtatttttcttggcctgt aaaaactaca ggtatttgca aaactgttct 91980 aaatagtaat gtaatagaagttttttaaaa attgagttag tgcatatgtt tggactcaat 92040 ttctccctct tttggttactatatatggtt ctaatttcca gctgtgcagt tactattgta 92100 aattctcaac attaaaaaaattttcagata caaattattt ttattgatac atacttttta 92160 catatttaag gggtacatgtgaattctcaa caattaatat taaataaaag gagaagaatt 92220 ctgttctatc ccagattagaaataccttag aatttctatt tgtgtctatt tccctccatt 92280 ccattatttt cttcctcttcataccacatt tatatacgtc ttaccattta tttatattta 92340 tttaatactt tacttcaggaaacttagtat tgttctggaa tcagaaagcc cttctagcta 92400 gtctgtcttg ccaggtctttccgattcttc tccctcaaga agcaagtggt ggtgaccacg 92460 tcgacataag agatttcccgtctgactcct cccaccatct gtgtaggtca cagaacctca 92520 cgccaactgg ctctcttcagactgtggtca actggaaaat cgagtcagat ttcagaaggg 92580 tgggaaaatg aacctagaaagtctaatgtg gagaaagcct taaaatatat ccttcatgtt 92640 ccacaatagt gattattatctttggcgata ctgcaacata aataggtatt cttggctagt 92700 tcatctctgg cggcaccatcttggcattta cactctacag aaaaggtacc tgaggagaaa 92760 gatggtccaa gttacttgccacagatcaca cagattaaca gcagcacagt ctgggtgtat 92820 atgtgtaata tatatctttttattattttt atatacaagt aatggttgtt tcttgtagaa 92880 aagttaaaac aatgaagatgagcaggaaga aaaaaatacc atccaaacac ccagcaaata 92940 accatactaa tgtagacatattttcttcta atcttctagg tatgtatgta tacatatatg 93000 tacatatata tacacatgcacacttttttc taaacccata ttgacctata acctcctttt 93060 ttttttactt aacaaaatactgggaatgtc tttttgtgtt tttaatattc agctattcca 93120 tagtattcca ttttataattatactgtaat ttaagtagcc agccctctcc ttttggacat 93180 atatgttgtg ccaagtgttgattacaataa gtatttctga aatgaacatt ctcacccata 93240 catcttggtg ctgcttttttttttttttct ataacccagg acagtgctga agtgcttctt 93300 ttctttaatt tcctcagttttagttcctag aagtggcaat gctgggctgt tgggtttaca 93360 catgtttaag gttctgatgcatgtgactaa attaccctcc agaaaagttc tgctttaagt 93420 tctacttaac gctttcccaccctgggacag ctgaagactg gccagagctt tgcagctgca 93480 atgatgtgtt taccacctcccttatccagc aacatttgca attagcctcc gaccttgttc 93540 ctgggattgt ttccggtgaaaattagccat gcctggctaa tttttgtatt tttagtagag 93600 acagggtttc accttattggtcaggctggt cttgaactcc tgacctcgtg atctgcccac 93660 ctcggcctcc caaagtgctgggtttgggaa tggtacctgt ggatgcacct agaggtgtgt 93720 ttagacacta gctgaaatcactttggtctt tctagcaaac cttagccaat gggctccact 93780 cacaaagggt cagcttccaagagcagattt tagctcaggg cctcttatgt tcaggacaat 93840 aatggagtaa aaaaacaaggctgaaaacca gaagatatgt gggcttaagc agatatgtga 93900 atagctaccc acataggaaaaacaaaacaa aacaaaacaa aaacaaaaac agaaaataag 93960 gtgggagtcc ttcttttacaaccatggata ccttttcttt tcttttcttt tttttttttt 94020 ttgagacagg atcgctcttttgcccaggct ggagtgcagt ggttcgatca tggctcactg 94080 cagcctcaat cccccaggctcaagcaatcc tccacctcag tgtcccaagt agctgggact 94140 acaagtatgt gccaccacacctggcctttt tttttttttt ttttttgaaa tggagtcttg 94200 ctctgtcacc caggctggagtgcagtggtg tgatcttggc tcactgcaac ctccacctcc 94260 caggttcaag caattctcctgcctcagcct cctgagtagc tgggactatg ggcgaacacc 94320 accatgcctg gctaatttttgtatttttac tagagacggg gtttcaccat attggtcagg 94380 ctggtcttga actcctgacctcatgatctg cccacctcag cctcccaaag tgctgggatt 94440 acaggcgtga gccactgcgcccagcctggc caattttttt aaatttactt tttgtagaga 94500 tgaggtctca ctatgttgcctaggttgatt tcaaactcct gaactcaagc gattctccca 94560 cctcggcctc ccaaagtgctcggattacag gcataagcct ctgtgcccag ccaatacctc 94620 ttattatcta actgagcattgttaaattca gggatgggtt gctgagggac gatatgacag 94680 attacactcg atttgatgtgtgctgactaa ggcctgtgca tgcctagcat tgctttagtc 94740 atggaatgaa tcacactcaggttcagattc tagtcctgcc acttcctatg tccttgagca 94800 agtgccttca tttatctgagactcactttc cttgtccata gttattccaa ttgcatttgg 94860 ttgcagtgag tagaaattaagtatttaggc tgggcacagt ggctcacacc tgtaatccca 94920 gcactttggg aggccgaggcaggtggatcg cttgagctcg ggatttcgag accagcctgg 94980 gcaacatggc aaaaccctgtctctacaaaa aatatataaa ttagctgggt gtggtggctc 95040 acacctgtgg tcccaactacttaggaggct aagacagaag gatgacttga gcctaggagg 95100 cagaggttgc agtgagctgagatcgtccca ctgcactgca gcctggggga cagagtgaga 95160 tcctgtcttt aaaaaaaaaaaaaagggaag aaatcaagta tttaaagcac ctaaggacag 95220 cccccagccc catggtagtgctcagcaagt gtttggtgaa tgaatggatt catgctctac 95280 gcttatatag agtttactttgctttcagat tgggttctgc ttgctcattg tacttatcac 95340 tatatgagaa ttgtattgtttattttcaaa tttgccacat attgtctgtt tctccatcaa 95400 cacaaaacat tctcaggagaagagttggtt cccagtgcct gaaaacagtg cctagcacac 95460 agaagatggt gaatcaatatttgtggttat ttctcactca cattccgtcc agcaacccag 95520 tcttgttacc aggaggctgttcagcacagg acacttatca caccatttgt tgaccctcca 95580 cttccccctc tgccacttatcacaccattg gttgacctgt gcttgcttcc agccatcctc 95640 cgttacctga agaacgatgggaaggtgcat ttggtggttt tcaacaacct gcagctggca 95700 gacggaaggc ggcacaggatcctcctgagg ctgagcaatt tgcagcgagg ggccggctcc 95760 ctagagctct acctggactgcatccaggtg gattccgttc acaatctccc cagggccttt 95820 gctggcccct cccagaaacctgagaccatt gaattgagga ctttccagag gaagccacag 95880 gtaggaaccc acaaaccattctctgaagtg gaaaaatgag ctgccagtgc ctagggagtg 95940 cagaggagcg tgctgagaaattcctgctgc aggtcagtgg gtctaatgcc gcttgcacat 96000 cagaatcacc tgcggagcttgaaaagcaag tgataggggg ctgcccctag gacaaactga 96060 atccgaatct gtcgggtggggcctagcata tttttatgct aggggcatca gcatattttt 96120 aaaataccaa ttctaatgtgatgccgaaat tgagagcttg tgctttcaat gataagtaga 96180 gactctggat acaattgtaacgttggaaag gaagggcaaa agtaaaaata actacctcag 96240 ggtgatgaca ttatctattttcaattttct ataatgaaaa taaaaaagaa tgattagaaa 96300 ctgccaagct ttcataacagcattttcaca ggtcttgtca gaacatccac taacaaaagg 96360 gagcttagct tagctgcatttggtcttaag ataccatcac ctctgtcttc tgttttactc 96420 caggttgatg gtatttttattattctgtca ttgcacctgc tgtagaaatt gccacataca 96480 tttcttttag aattttgtgctaggcagtgc tttgttttct ataaagaact agtattcttt 96540 gtcctttcca ataatgtataactggttaaa gttcctgaat ttcatccgct atataagcca 96600 atgtcaaatc agtaactatgttcttctgta atggtccata ttatttttgt ctgctacctc 96660 tcaatcctca ctttccatttgttaattcat tatattaata tatgctttag ttacaagcta 96720 ctctgagccc tttgtacagagaagcagggt gtaagcaata atttccattg caagtaggaa 96780 tgctgtgcag ttttagatagtaatataaaa actatgaatc tgatcctaga agttttgccc 96840 ttgaacaaca ggaagcatttttaatcagaa attccaagaa agctaaaatc cgaggttttt 96900 ggggattttt gtttttcagtcatatataga gagattttta atgtagattt acatatggaa 96960 aaaaattaat agaaagatatgctaaactct ctttctgcat ccccatagta gcgcatgtaa 97020 tcagaatgac agtcagaaattgtgtcactg cttatttcag aaattagtgt tcaccaccaa 97080 ataagtcaat ttctaaaagtcaggactcac ccctgacaaa gtcatcagaa actgtactta 97140 ctggaatttt ctcagaagctctacctcctt agaccatggt agtgatcaca gtggctcagt 97200 ctcataatca cctactcactcattcagtca gtcaacaaat gggcacattc taggcattag 97260 tgctaccatg gtgggcaaagccagtcattt tcctgactct gcggagatta tagtctgatg 97320 tccaatacaa tatgtgttaaatagcacatg ttgcctaaat cttagctaaa tcaaaaagca 97380 gaattcctct gaaaatccttagctgtgctc atttctaaaa taaggatgat gacttcactt 97440 tggcagttaa cataaatgttttgttgtata tagatataat aaaggtgatt gcaagcagca 97500 ttcacaagat aaaagggcaaggacattgac tttttgacta acaatgaata atatcaaccc 97560 gtaatataaa aatatgatgccaactaccta atgatcatag aagatatcat ttattcattc 97620 tcatatccca aagaagtcagaggctttgct agacccttga gccaagacaa ccaaaaaata 97680 atactgttta aaattggggttaacacctta tttattatta tctaaattcc ataaacaaat 97740 tacagacaat tcttaagaggctcctgccac aaaataactc ctttaccatg taggaaaaga 97800 gcctttcgtt ttctcagctgtcttggtgac aatgccagca ttttcctagg ctactggcta 97860 cttacatatt cactttagtcaaaaagatag ggctggcagg ctttaggaaa aaatgtgatc 97920 ctacaaaatt atgtaagatgtcagagggtc caagtataca ggactacttg attatgaaaa 97980 ttgcgtgagt aggcaagcacattggctttt gaacagtgtc agcaatctgt ggtatgaatg 98040 tgatttcttc caggacttcttggaagagct gaagctggtg gtgagaggct cactgttcca 98100 ggtggccagc ctgcaagactgcttcctgca gcagagtgag ccactggctg ccacaggcac 98160 aggtgtgggc tcttgggcagtttgcatgcc ttcatcaaca caaactccaa agaccctgaa 98220 taggctgggt cccatcaccgaaaagtggga ctgtcaacag agcagcccaa caaaacgtat 98280 ctatgaaaat cagtctcttgaacaaaatcc agggcacaga atattttcat tatgctttta 98340 tttttatgat tcacatggaatttagactta gtcagtggca tcattatcat tatttttttt 98400 agctgaatta aagaaattaccccaaattaa attcaaagat tctgggtttt tgaatcatct 98460 tgtcaggatg atttcctggggaataatttg gttttgagtc acttagaaat gaccatcact 98520 ggctgaaaac tctttgccttttgctgttcc tacaggggac tttaaccggc agttcttggg 98580 tcaaatgaca caattaaaccaactcctggg agaggtgaag gaccttctga gacagcaggt 98640 aacaagcggg acatatcatcagaaaagccc tcgtcttctt agagcagctc cccacaagct 98700 agtggagctg caggcctgcccaggcacggg ggcagcctct gagccagatc tccgggggcc 98760 cacgcagccc cgcacgccagggctctgctg gaagttctct ggaaatgggg tccaagctcg 98820 tgatgaacac accatggctctaagcagagc gggagccatc aagggctgct aacgctactg 98880 taagaagtgt tacagagagccaggtgcggt gcctcatgac tgtaatccca gcactttggg 98940 aggctgaggt gggcagatcacgaggtgagg agctcgagac cagcctgaac aacatggtga 99000 aaccccgtct ctactaaaaatacaaaaatt agccaggcat ggtggtgcat gcctataatc 99060 ccagctactc aggaggctgaggcaggagaa ttgctggaac ccaggaggcg gaggttgtag 99120 tgagctgaga tcgtgccactgcactccagc ctgggcgaca gactgagact gtctcaaaaa 99180 aaaaaaaaaa aaaagtgttacatagatagc tccacatgga ttctttcatt cctggatgat 99240 atcaggcatt ccttttcaatccttttttcc ccttctcatt tttaaaggtt aaggaaacat 99300 catttttgcg aaacaccatagctgaatgcc aggcttgcgg taagtgcttt tctagtaatg 99360 ggctcgcctg agttggatgatgcaggctga tgaagggctt gcggtgaggc tgtgttgacc 99420 gcagtttctg aaggtctaccacatagttgg aggggaggga ggaaaagttc aataggaaac 99480 caaattttgt tttgccttctgtatatcttc ctggcggtga atacgcctgt ggatgattgt 99540 ttttctctag gtcctctcaagtttcagtct ccgaccccaa gcacggtggt gcccccggct 99600 ccccctgcac cgccaacacgcccacctcgt cggtgtgact ccaacccatg tttccgaggt 99660 gtccaatgta ccgacagtagagatggcttc cagtgtgggc cctgccccga gggctacaca 99720 ggaaacggga tcacctgtattgatgttgat gaggtaaaag ttcacttaga ctgggagggg 99780 atccctaagt atcctcctcaccctgcagct tttggaaaaa gaagccaagc tgatgattaa 99840 gggctgactt gggctgtcctccaggctcca ttgaaaccac ttgcttggag accctgagac 99900 aagggatggc ggaaggagacatgacccaag gttatggctg cgtttgtcat ttttcctctg 99960 tgtttccaac tcttctactggtccattggc tacagtgctg aggtttctgc tttacacgct 100020 gacatttgca ttggttatttctcttaaata gtctgtgcta caattttgtt tttcccccac 100080 ttttgcaaat gtggaaggggaggatttttc taagggtata tttcaggagt caccgtggat 100140 ttcttcaggc ttcctaaaataaaggagcag aggggaatct cacaccaaca agggggaggc 100200 cctgtaacct atgagtaactaaacgtgccc agggagggcc ccagaggagg cacatccagc 100260 aaataccagc tatttgaccggctggcagag ccccagaaga gactgtttca agaccctggt 100320 taggcaggcc agcctagtggctgaagcctg caatctcaac actttgggag gctgaggcag 100380 gaggatcaca tgagcccaggagtttgagac cagcccgggc aacaagacaa aaccctatct 100440 ctacaaaaaa tatgataattactggggcat ggtggcatgt gcctgtggtc ccagctactc 100500 tggaagatga agcaggaggattgcctgagg caggaggttg aggctgcagt gagccatgat 100560 ggcaccactg cactctagcctgggcacaac cctgtctcaa aaaaacaaac aaaaaaaaac 100620 atggttaggc aatcaccagtccagttgcaa agcaatgttc atagcaggta gcaaggctgc 100680 caccaaaatg tgccttcaatgtgcctttct cctcccaccc agcccaagga aggctgaaag 100740 gagaagcgca ggggagagatgcaggtacat aacttaggca cactgactcc ctggggctcc 100800 tgcaggccca tggtaatgttagtaacactc agctgattac cttcgctagt agtaagcaat 100860 taagacggtc tctagtaatccctcaattgg agaaacaact gcatacattt cactgaactg 100920 catctggttt ttatctgtgaggtagaattt tcctctttct gaaagggcag gaagtggtca 100980 tgttttttac attagttgaagtggtttaaa aaaaacaaaa aacaatctcc aagattccta 101040 gagccttgga atagcatggacttgacagga catggaggca ggcacaggcc ctgagggatt 101100 acatggtaat gaagaggcaccggccctggc tttgccttct cgaggtcttt ctgattgagt 101160 agttggtttg ctgatgcttttggaggcatc taacagccag ctaagtggtc cagggacata 101220 gtcctttcac agagatttgcaaattccatg tcagaatatc agggagctta ttacattgag 101280 aggtaggctg gttaatatcaaggacccttt caacatgcat gtgccattat tctacagcac 101340 agcaaagcct actaaaaagcaacctcagat tgtgtggtca gggattcagt gggtaactgg 101400 agttggaagc ctttatttccttttattatt ttttccacca aaagatagtt aaatgactaa 101460 acaagtatgt gcaaatagatgaagaaaagt ttgagagtgt tttctcggtg tggctaaaga 101520 ctttgaactt ttttgtctccagtgcaaata ccatccctgc tacccgggcg tgcactgcat 101580 aaatttgtct cctggcttcagatgtgacgc ctgcccagtg ggcttcacag ggcccatggt 101640 gcagggtgtt gggatcagttttgccaagtc aaacaagcag gtaggctgaa gtcatggttt 101700 ctattcattt ctcatcttaacaaaacaact gtggttggtt cccacctctt tggtataaac 101760 ctttacctcc atgagcagtcaaggccatgt ggaatgtcat ggtgcaaaat gagcaaatta 101820 ggaagttttt caaccatttctgagtattta catttataaa atctagaagg gaggaagctt 101880 ttaagagggg ataatagagtatcactagaa aaacagtaaa tggggtaata tggctattgg 101940 cataatttca gtcttattttgttgatagct gttgagcatt ttgtcctgca gaataagttg 102000 agaaccacgg agacaaaagctttgaaaaat taggtcactt ttgtagagtc attgttgcca 102060 atcgaagctg tttcagagatgaggggattt taagagtaga aataatcccc caaggcaacc 102120 tccccttttt cttatgtaagctataaattg ccataaagca cttgagtact tcttgacagt 102180 ctccatttat ttcccaatgctctactatat tctggtcatt atttaactga ctgactgtga 102240 aattcttttt gatgtttttagcccttaaaa atggttgttc tataaatata ttttactacc 102300 ttagggaaat tattagctttgaagaagggc aggggtttat tatttcctag ctgcctatca 102360 aagggttcta gagggcatgatttttttaag ttattgttat actttaagtt ctagggtaca 102420 tatgcacaac gtgcaggtttgttacatatg tatacatgtg ccatactggt gtgctgcacc 102480 tgttaacttg tcatttacattaggtatatc tcctaatgct atccttcccc cctcccccta 102540 ccccatgaca ggccctggtgtgtgacattc cccaccctgt gtccaagtgt tctcattgtt 102600 caattcccaa ctaagagtgagaacatgcgg tgtttggttt tctatccttg cgatagtttg 102660 ctcagaatga tggtttctagcttcatccat gtccctacaa aggacatgaa ctcatccttt 102720 tttatggctg cataatattccatggtgtat atgtgccaca ttttcttaat ccagtctatc 102780 attgatggac atttgggttggttccaagtc tttgctattg tgaatagtgc cacaataaat 102840 atacgtgtcc atgtgtctttatagcagcat gatttataag cctttgggta tatgcccagt 102900 aatgggatgg ctgggtcaaatcgtatttct agttctagat ccttgaggaa tcgccacact 102960 gtcttccaca atggctgaactagtttacag tcccaccaac agtgtaaaag tgttcctatt 103020 tctccacatt ctcttcagcacctattgttt cctgactttt taatgatcgc cattctaact 103080 ggtgtgagaa ggcatctcattgtggttttg atttgcattt ctctgatggc cagtaatggt 103140 gagcattttt tcatgtgtctgttggctgca taactgtctt gagaagtgtc tgttcatatc 103200 ctttgcccac tttttgatggggttgtttga tttttttcct ataaatttgt ttaagttaag 103260 ttctttgtag attctggatattagcccttt gtcagatgga tagattgtaa aaatgttctc 103320 ccattctgta ggttgcctgttcactctgat ggtagtttct tttgctgtgc agaagctctt 103380 gagtttaatt agatcccatttgtcaatttt ggcttttgtt gccattgctt taggtgtttt 103440 aatcatgaag tccttggagggcatgatttt ttagactaga aaagggaggc cagctagaca 103500 tataggaatg ataagaagctgttaagagtc ctctaaagac aaaatctcta agaacaaacc 103560 aaggaaacat aaggtacttggtgtaaggta gtaaggagca cttctcaata gatttccagt 103620 aacaaggcag acagaactgaagtggaaggg agccaccttg ctaaatttaa cagcaaatgt 103680 tactgcattc caaagtcaaagaaaataaaa ggaaatggcg cagtgtccag aaacaaagag 103740 aaaactcaaa gccagaactgtgactagagc tgatgccaca gcagatcctg ggaggtggga 103800 gttgggtcca ggtcttagtaacagggacct ggcttttacc acctgtgtgg agtggatgca 103860 gcaatgtagt ctgggaactttggaaaggcc taacacccct gcataagtcc aggaccctca 103920 gagaaccacc ttatcaattacaaggagaca ggaaaccttt agtagcccta agaaacaaca 103980 aagaagctct ttagctttaatacatggttg acaaaaatga aaattgccta cagcaaatca 104040 aagcaccaca cttgtaccacatacagttat gagttccaaa tctgttatac atataatgca 104100 gaaacacaag ccaaaaaattaaaataataa ccagccatga agaagtgaaa tccccagggc 104160 tctgactcaa gtcaaaggcacaccactctg taaagacatg ccaaaattca gggccaatgg 104220 aatgcccaca ggcaaaatttgggctactga agatatgctt ataaacaaaa atggcagttt 104280 atataaagaa actcccatgaggaaacatga taaatgcaaa gtaaatataa aaaggtaaag 104340 aaataaatac catacttaaagaatagggca atgccccaac tgggcaacat ggcaaaatcc 104400 catccctaca aaaaatacacacaaaaaaat tagccgggtg tgatggtgca tgcctgtggt 104460 cccagcaacc ttgggaggttgaggtgggag gatcacctga gtctgggagg tcgaggctgc 104520 cgtgagctgt gattgtgccactgcactcca gcctgagcga cagagcaaga ccctgtctca 104580 acaacaacaa caaaaaaatatagggcaatg tgagaaaaac agataagttt gtaaaacaga 104640 caaataaaac ttttatcaatggaaaatcgt tgaacttaat agatgggcaa aacagattag 104700 acacagctta gcagggagttcatgggctag gaggtgaacc taaagaaatc atctagaatg 104760 cagcacagag aaataacgacatggagcata taaacaagag gttaggagtc aaaagaagat 104820 attaacaaga aagttcgatattcatccaat tgtagttcca aagaaagact agaaagcagt 104880 cgggggaagc tgagaatgttccagaattga aaaaagctaa gaatcctcat ttaggagaac 104940 tgcattgatt cccaagcaagattaaaaaaa aaaataaaac tttcctaacc taaaaaggtg 105000 atggaacaga tgaattatacaagttgtctt ttctgaataa ctttcaatac catgaaagca 105060 attctcattt gcaattagattaagtacaaa ctggtacaaa tgcaagggag atggcacaaa 105120 taaccctcag attaccattctacccgaaat tccgcatctt cacttcacac aaagatagca 105180 aaacaaagga gatttatttgcatttctatt taagcagcat gtctcgctaa actttctttg 105240 aacttttctg cactaggtctgcactgacat tgatgagtgt cgaaatggag cgtgcgttcc 105300 caactcgatc tgcgttaatactttggtaag tatttctcac agctgttgtt atcaaagcag 105360 aaccggttat taaaacaagtgtatgtttat agatcatagc ttccaaacac acctagaaca 105420 tgtgggcata atatatttgattggcagaat aaaggcaaaa gtttgtgctg aaaaagtgtt 105480 taatatgagt aattttaaaagtaattttta tgtaagaata attttataag tgccaagttc 105540 tttattttct atcaaagttgcaatattagg ctattagata actatttatt gagtaaataa 105600 ttattactcc tgtattttaaaattgtgaag agcttggtag tttatattaa tagttcagta 105660 ttttactggt gactacacaatccccaaatg gcattattta ttttcataaa actttcaatc 105720 atggggtcag gagatcgagaccatcctggc taacatggtt aaaccccgtc tctactaaaa 105780 atagaaaaca aaattagccgggcctggtgg cgggtgcctg tagtcccagc tactctggag 105840 gctgaagcag gagaatgacatgaacccggc aggcggagct tgcagtgagc cgagatcgca 105900 ccactgcact ccagcctgggcgacagagca agactccctc tcaaaaaaaa aaaaaaaaaa 105960 aaacttttca aaacttttcattgtattaaa ttaaagtgct tagcacttag ggatgcctat 106020 aacttaagta ttcagatatgggttttattt ctgaaagttc ctgtcaaact gtgtccaaga 106080 tgagagatgt accagagttgactatcaaga aaagaactac tgattacgtg tatgactatt 106140 tgaaagttat ccagcctctaggttccttgt ttccttgtaa gtaaaaacac tttacattta 106200 tcaagtcact tattttaatcctcacaacaa cctcataaac agggattatt attatcccca 106260 ttttacaatt gaagaaactgagggacagaa atattacatc attaagtcca ggcatggtgg 106320 agcatgccta tagtcccagctcctcaggag gctgaggccg aaggatcatt tgggtacaag 106380 agtagtttga ggctacagtgagctgtgatc gccactgcac tctaacctgg gtgacagagg 106440 gagaccctgt ctccaaaaagaaaaaaaaga aatgttacgt atttagacta aggtcacaca 106500 tcttccaggt tggctacagagcctgagttc ttctccgcaa tgctatattg cttaggaata 106560 gatggggatt tattgcttctcagcttttac caggacaaag tagtctgcaa atagatttgc 106620 gagatcttgg tgtaaaggtaataactgagc cacctagaga gaaggaaagt agacagtaaa 106680 tagaactcca ggaaaccccaaaaacgaagg actaggccaa ggaaaaagag cagaagccag 106740 aaaaaggagc gaactgatagcacagccagg gaggctgaaa gatcgagaca agtcattgga 106800 aagcatgcat tggatttggcaactaagagg ttgtgagtga cttaaatcat taaacaatat 106860 atatacttca aatggatgtcttttacgacc tgtaaatgtt atgccaataa aactgcataa 106920 gatgaaaaag atctaatgtacagcatggtg actgtagtta ataatactat attatatata 106980 tgaaatctgc taagagagcagatcttaagt gttctcactg aaaaagaaaa aaaggaaggt 107040 aactatgtca gttgatggatgtgttactta actgactgtg gtaatcattt cacaatgtat 107100 acgtatatca catcatcacactgcacactt taaatatatt cagttttgtc aattatacct 107160 caataaagat gaaaattttaattttcattt tttaaacata ttgtataata tttattacat 107220 atatagtgtg cagtgtgataatatatatta tatatataaa agctaaaaaa acctggcccc 107280 tgaagtgtca ttgcagcggagctgacagct catttgtcag tttctcagcc tacctcatca 107340 gaaattctca atgcaggccaccagcatttc tctgtgcacc cattcctttg tcattccatt 107400 gtaaaagagc caaaggaacagcaataagaa aaattaactt gctttttaag cagatcatct 107460 ccctgtcccc agagtaaacttctcacacca cctcccagga cttcactgga acttttgacc 107520 tgtcccctat gccctccacggtggcgcggg caactcagtg tcattggttc acagtccagc 107580 cttcttcctt cacttctattccttctttgt ctgagtggcc ggacgcagcc tccagtctga 107640 tggtgttttc atcaacattcacatgatctt acatgtaagc aaagaagaag gcatggatct 107700 gatgcctgat tcctgccagcaatgagtacc tgtgaaaata tacacaataa ctgtaccttt 107760 gctcaaactg tacctttgcccacagctttc agctcatcac ctgatctttg ttccttgcag 107820 ggatcttacc gctgtgggccttgtaagccg gggtatactg gtgatcagat aaggggatgc 107880 aaagcggaaa gaaactgcagaaacccagag ctgaaccctt gcagtgtgaa tgcccagtgc 107940 attgaagaga ggcagggggatgtgacatgt gtggtaagtt gttttttgac ttcctctatc 108000 atttttcctc ttccatttttaacaccacct ctctccagtt tctatgtgct tcagggtaaa 108060 agtactccaa aatgaaagcattgcaattaa atgagcatgg agtgtaatag gaacagggtc 108120 caccgagttt gtggggagcagcctgacatc catctaacca tcagctgcct tttctctccc 108180 tcttggctgg ggggcaaccctgtccacttg ggtacctgga tgccagagcc tccggtgaag 108240 ccccagctcc caagcaccttctatggaaag gcccagaagc cagcagcagg gaggtgaagg 108300 aacattcttc tcccctttccttcttctgcc ccatctacct gcctgtataa tgacaacata 108360 cctgctgtca tctctaactgtcctcatcct tacaacttcc agagtctgtc tgccttgacg 108420 ctcctgttgg gggtgggccatcgtgtagag gtggggactg tcatagtccc tccgggagaa 108480 accttccgtc tttggcaggtgactggcagg gccagacggc gccatgctgt atgtctacct 108540 ccactccaat cagggacagctccatgaatg gtcacgcttc ctgtgcattc tccagccacg 108600 gagctggtct ggggaggggaggctgggaag agagtagagc agtcacagca gggcctgaca 108660 tgggtcccat gctgagcttgtagtgggccc tatgtgaagg agagggcatg ctcctgccat 108720 ccattcccaa atccaaggatccagagtcct cagcctgcca tccattccca aatccaagga 108780 tccagagtcc tcagcctgccatccattccc aagtccaagg atccagagtc ctcagcctgc 108840 catccattcc caaatccaaggatccaaagt cctggggatc ctaggcactg aaaaatcagc 108900 agacttcagc tgcccccctcctctgcactt cctgggatgc caggccctcg gccggtggcc 108960 cgtcagactc tggggtcactggttcacagt gatcacccct gagtagcaag atggattctc 109020 ggagcccttt tccatctttatctcaaagtt atgcttcttt atctttatgc atacacaatt 109080 ttccacaatg agcctaccacttttcaaata ggaaaaataa gagtcaaaaa tgagttaata 109140 aataaatata atgaagtcagccctccttgg caatacagat attctgtaac aaggtcagat 109200 ggaagaatgt ggtgtatcagagggggaata accccagcaa tttcctgaat tcctcatggt 109260 tgggcttgtt tattaccaaaaagcagtcat tgagaaacaa gtgtatatct ctgttgaaat 109320 ccccattttc tgtgaacaaattgacaactc ccctgggatg atgggtgatg gggctcttaa 109380 tatgtttttg aatttgtaataaggcacaac gacagatggt aaaggattaa taaacttggc 109440 gtgactacta aaataattgaatctgccaga aaatagctca ttaaaaatgt atttgttcct 109500 agaggacatt ttttcctcattaaagtgagt caaggatcag cctcgtggat cagccttcca 109560 ggccttccaa aggccctgctggggcaagtg agcaggaacc aggagcccgg gagggtctct 109620 ctggagttct gcctcctaaatcctggttgc agagggagag cacagatgat tgagaataag 109680 caggctgtgg ctgaggaagtcactggtctg aaagcttttg ggtggtgagg ccaggaaatt 109740 taacctgtgc tcacataagtctgatttccc ttggcccaga ctctgctcct cagactctct 109800 ccctcttcct tcctttacctcccgtcccag gtcagctttc tctccctctg cgtttccagt 109860 tccctgtgac cttctgggtcccctcctccc atctcccttc tgctgctctg cctgttcctg 109920 gaagtgggag tgggagggagagttttctct ctgagtctcc ctttatatct ttgaatagtg 109980 aaccattaca ctattcaagtgcaattaaat agtttctaag ccccacccaa caaggctgag 110040 tgactctggg ccctccccctgggattgagc aaaggagcag agaggccctt gtcagccacc 110100 agcttcctgc caggctcagcttcccaggcc tctgatgggc tttcccttta cagtgtggag 110160 tcggttgggc tggagatggctatatctgtg gaaaggatgt ggacatcgac agttaccccg 110220 acgaagaact gccatgctctgccaggaact gtaaaaaggt aaggggtgtg gggtggcagt 110280 aggcacacat gcactgtggctttccaaagt cacatcttct atggggagag gttctgtgga 110340 tctaatttaa aattgtacttgtaaagtagc tgcatctcag aggaagtgaa acagccacca 110400 acaataaagc cacagtgtcttgaggtcaga aacagttact ggcttaacac aaatgtagga 110460 tgtcactcgg aactgcattttccccctaag gacaactgca aatatgtgcc aaattctggc 110520 caagaagatg cagacagagatggcattggc gacgcttgtg acgaggatgc tgacggagat 110580 gggatcctga atgagcaggtacctgcttcg ctgggagggc ctgtgaattg ccacgtacca 110640 gggatgatgg gagaaagcctgtgatgtcaa ctatgtatat gaatcatccc aaatgttagt 110700 ttccatgcct gcattccacagcactgcagc tttttaccct ataagattca cagatgtttg 110760 aaatataaaa ctgacaaacttgggccacca gagtgctgat gcttcacaac aatggaggaa 110820 gtggatttag taaaagtctgagtgatgttc tgtcctttca tcttttagga taactgtgtc 110880 ctgattcata atgtggaccaaaggaacagc gataaagata tctttgggga tgcctgtgat 110940 aactgcctga gtgtcttaaataacgaccag aaagacaccg atggggatgg aagaggagat 111000 gcctgtgatg atgacatggatggagatggt agatttatct tgcttttgtc ttttatttgg 111060 aattcagttg accttgttccattgttctct gagagagtag gaatagaatg actgattcgg 111120 agccttccag ctgtgggtggctcattgtgg cctgcatctg gaagacccaa ggtggacttg 111180 tgtctatgga aggagaccccacagcccact ccactaccta atggctctgg atgtttccag 111240 gactctgtat cattgccagaaactttgtat gaccccttca agcagtgtct tgtcagagtg 111300 tgtctttatg gggtatctcccttcccattt ctacctgtcc tcactctcca ctatcctttc 111360 tacatgtgag agacccagaagtggagaact gaatgagaag gcattgagca gggtcacaga 111420 ctatgcatca caggctacacatcccattgt gggcccttga aggaaaaacc tttttgaaat 111480 accacctgcc ccaacacacacatacacaca cacacacaca cacacacaca cacatacacc 111540 actccagaac atgcacacccccctaaaaca tgacctcacc ttctgcaagc ctaactgatg 111600 acccatttcc agtggtatatgctggtaagc aaatcacaga aagccctagt tggtagcatt 111660 ttccagtttc catgatgtaactccgcattg tcctcattgc tgatttcaag ctaccaatgt 111720 gacatcatca aacacaaaactgaaaagaga gggacacaat ggacccagct ccagcacaac 111780 actgcctaga gccctagggtctcagtcata gctcagatac ccacgagatc ctccctctcc 111840 agggtcagcc atacagccatcatgatcccc tcctcgtggt tgctatcata gcccttgaca 111900 tcttggacaa attagaattcagtcgataaa tacctctgag agctgttcat ttttaagtct 111960 cccagaatcc gtcctttgaagctacctctg gataactgca gaaaagggta gtctctgtgt 112020 gacactggcc caggcagaagtgactcacca tgcgcctgat ctctccagca cctagaagaa 112080 gtcagtgaca ttcccctgactcaaggtagc atttctttct cacaggaata aaaaacattc 112140 tggacaactg cccaaaatttcccaatcgtg accaacggga caaggatggt gatggtgtgg 112200 gggatgcctg tgacagttgtcctgatgtca gcaaccctaa ccaggttagt aggatactgg 112260 ggaattcaaa ctccaggctgaattcctcta tgcaaagact catttaagac gggtgtctag 112320 aaaaacagca gagcctcttagctgttgcca cttacctaac aaagcagagg tggaaaaatg 112380 acacactgtg ggcctgaagatggtgggatg aacaccacac aagatctgaa gccctaggga 112440 ctatctccca ggctgtcgattgcctgtgag gttagtaaca cgaacttcat gatgctcaat 112500 acaagtattt ttcctgcagaaaggaaaacg gagcatggaa attaaccatt gggagccttg 112560 tgtcccttta gtccctaaacccctacacag cctatgtatg gacatttcag attaagttca 112620 gccagggatg tgttgcatctcccttctcac tcctcctccc tgtctggata attgggtatg 112680 atgtgtcatt tatttttaaagccctgagag aggggagtca tggttgccct ctgtgactca 112740 ctcatgattt tggccaaattgcaaattgca gaagcaaagc aaaatcaacc tgccttctcc 112800 ccatgcctat ggctggctccctgtgacccg tgccagagtt ccatgcctcc acctgccacc 112860 atgctacccc agcgcccctttccaatcagc tcatctggtg tgtgggcagc ccatccatgt 112920 agcttgatat tttagaaggcttttttgaaa attcagcatt gtgcctacac caatatggtg 112980 ttgtcctttt gcagttttgcaccaccccgg ttccagagaa ataattcccc aaatccaatg 113040 atgatgggtg aggtctgccttctctggaac attcggtaca tgcaggccca ggaataaata 113100 acatgtgctg ttctctttgcagtctgatgt ggataatgat ctggttgggg actcctgtga 113160 caccaatcag gacaggtatggcatgtctcc caactgcaga gagacagatg caaacatccg 113220 gagagggttt ttggtttgtttttttttttg aggcggagtc tcactctatc acccaggctg 113280 gagtacagtg gtgcaatctcggctcactgc aagctccgat tcccaggttt atgccattct 113340 tctgcctcag cctcccaagtagctgggact acaggcgccc gctaccatgc ctggctaatt 113400 tttttgtatt ttcagtagagacggggtttc accatgttag ccaggatggt ctcgatctcc 113460 tgaccttgtg atctgcccgcctcggcctcc caaagtgctg ggattacagg cgtgagccac 113520 tgtgcccagc ccggagagggttttaaattt cagaacttcc tgctgctcca ttccttggga 113580 tctttctggg tggactgagctgctggtggg ataatagccc tgcctctggt cagaattacc 113640 atgaaaattc tccctgaaagttctgtatag ggtataacgg tgtcagaata ggttactagg 113700 cactccagca agtgagatctatttcaggag aggtaatata gcatcatgct tcacagatgg 113760 gctctggaat aagacagctagatataaatt tcagctcctt ctcttacatg ccagctaacc 113820 ttaggcaaat tacctaatacttttgcctca attttctcat ctgtaaaatg aggatgacaa 113880 tagatctagc tcataggaccatcgtgaaga ttgagttgat ccatggaaag tgctcattat 113940 ggtttctgtt atatacatagcactcaatag acattagctc ttttattttt atattctttc 114000 agctcaggaa aacttcaccatcatcccaaa atagaaggct gtcagtttgc tgagggattg 114060 ctattatttc ctggtccttgaaattttttt aattcagttt tccttattta agtggaattg 114120 ttctgtggaa gtgagttttgtgaaccctcc tagctcaaca cgatggagtc ccacattaag 114180 actcaaggct ggatcaactgatcagattct ggaagaacat caccaaaaac agacgtgtgt 114240 cactcagccc tagcggcacctgcaacccaa ggttctaaac ccttttgtct ccatagggaa 114300 ttcttacaaa ggcagccaggacaaggatga gagggaaagg attctaaaag agaaagcaaa 114360 aaaagatgca atttctctcctgatacctgg atcagaggtt gccctgcaga aaaccagttc 114420 ctgacccaaa ccttactcaaggacagctcc tatgaagcca cagctctctc tctctttttt 114480 tttttttttg agatggagtcttgctctgtc acccaggctg gcgtgatctc ggctcactgc 114540 aacctccacc tcctgggttcaagcaattct catgcctcag actcccacgt acctgggatt 114600 acaggtgtgt gccaccatgctggctaattt ttgtattttt agtagagaca gagtttcacc 114660 atgttggcca ggctggtctcgaactcctgg cctcaagtga tccacctgcc tcatcctccc 114720 aaagtgtgca tctctttcattatgctctgt gtccttgctg caaagtacat atagtcatcc 114780 ctcagtgtcc cagggtattggtcccaggat acccctctgc ccccaggata ccaaaatcct 114840 caaatactca agtcccttatataaaatgat gtaatatctg catataacct atacctgtcc 114900 tcctatatgc tttaaaaatcctctctagat tacttaatac ctaatacaat gtaaatgcta 114960 ggtaaatagt tattatactgcattggtttc ttatttgtat ttttttggtg ttatttttta 115020 ttgttctttt tcaaatcttttcaatcctca gttggttgaa tctgcggata cggaaccgcg 115080 gatacagagg cctgactgtactagctaagt gaacttaaac atatttccca gtctcttcac 115140 atctcaactt cttcatctgcaaagaggaga taaataaatg catcgcacag gttatggtga 115200 aaaatcaaga taatgtaggtaaagctcccg gcacagtgct ggcccaaggg tgacatgcag 115260 aaaacagggg ccgtgttattattggtatca cagtacaagg aaaggcacat cagcaacaga 115320 gcctgacatc agatgtccctctcctcttcc gagaggaagg tgggactgta tcctgacaac 115380 tgccctaaat gatgtgcgtcaagaatgttc cctggtgcag gtcatcttgc actctgacta 115440 ctatctacat taatttctaaaatatcctgt cccaccagcc tctagatatt tgcccaaaac 115500 aaatgactca ttctgctatgttttaattcc cacaaagttg aaccttggag aagaggatag 115560 gaactgcttg tttttgcataaaaatgttca gggctacata gaacggctgc taatggaaat 115620 agctcagtct tgtgttttataaagcagacc tgctgcatgc acagggattc tgctgtttga 115680 gccacatcat tttctgcttgtcaaggctga cttcactttc actggaccct ttctgtcccc 115740 ctgtaccatg tggtaggtgtggccctagag catggtctgg tccctggctt ccctctccaa 115800 aagggtgcag gggcagctcctggcccagga caggccgcag gtggaccagg tctaaaggag 115860 tgctcattct tttccagagtaaccatgaga ggtctcagac tccaggacac ctcttggagg 115920 cagggcagac tcatcttcaggtggcccctc agcccagaaa gtcaggtgga cagagaagga 115980 gagttctgag aagtcctttagaccagcact gagcccggcc tacacactct cctcgagaag 116040 gcgtggccct gttcactttgcccttctccc catggcgttc aggtctgaat cctcattgga 116100 tccactagcc agggattgtgaagagtaata taacggaaaa cactgtaggg cttcctaaga 116160 gtcagcactt tttcatatattgaaatttaa tatgcaacat tctgattttc atcatttaat 116220 ctccataaca actctatgaggtaggtacta ttctcatttt acagatgagg acactgagcc 116280 atagagaggt caggtgtcatgcctgaggtc acacgctggg cacagtggct gagccaggat 116340 ttacactgag gctgtctggttccactgcct gtcattgttc ccctcacaca tcctctgagt 116400 tgagtatcat ctagcagttcccagggactg actgatagga gcatctggtc ataaatcata 116460 gaaatagacc cttatcttctttagtaggaa atgactcact tgttttaaga ggttggggat 116520 gggaaaattg tctctgaggcccatagggca gccttatcta agggaatgac cccagtgggt 116580 ttgattttaa gagccaaccctggtttaaaa aaaacctcaa gcacagactc tccttcctaa 116640 aaatggatcc ttcctgttccctgctgaact gtgagccaca tcacctgtcc tttctcctgc 116700 agtgatggag atgggcaccaggacagcaca gacaactgcc ccaccgtcat taacagtgcc 116760 cagctggaca ccgataaggatggaattggt gacgagtgtg atgatgatga tgacaatgat 116820 ggtatcccag acctggtgccccctggacca gacaactgcc ggctggtccc caacccagcc 116880 caggaggata gcaacagtaagcaggctcag cccagagctg cacagcaccc ctgggctccc 116940 ttcagccagg cacatgggggcacgcctctc tccaggcacc aactcagaat atccccagct 117000 cccatcagct gcttctctacacccagcttc tctgcatagc tgcagcttgt gtggacagca 117060 gatatctggt agacaccagtctccaactca gtcaggactg agctgctttg catcttcctg 117120 aacaacccta ggatgccatcacacaggtca gggaagcagc cacacactgt ggctcagtcc 117180 ccaaagggct tggagagctgctccaccatc ttacctgtca cctgccacct tccactggga 117240 gtcccagaaa aggagcaggatagagagaat ggcacagtag aagtggtata tgctgggttc 117300 acaaacacag atgcctgcaggtgccaggca gataagaaaa tgtctgcacc aagccagaca 117360 tgagacagca gggagtgttggggactgaag tgacctggag agggcatgcc catccacagc 117420 agtgcacatg caaaaatgccagcacgttag ctgaacaaca cacacgtggg ggccaccaga 117480 agttagtgtt agagatattctggtaacaat aatttccctg ttttctacac attacagtcc 117540 agaaagtgct gtagacatttgcttatgtga ggtcaagctt aagatccacc agttggtgaa 117600 ctcaactgta ggtaataagaacttcttgag gacagagtgg agggcaactg ctcaaattga 117660 tctttcaaaa aactctgggaggactggcct ggtcacccag tacaaggact ccagaatggg 117720 gcctggcacc agctcagacatgtccccagg gctcctctcc caacactgat gggaaatcat 117780 ggccctggtt gtgggagcttgagcccttct gttcctcatg cagccccctt ccctgccaag 117840 gagtggcggt gggtgtgagcgctattgagc tcctgtcctt tctccacccc actcaggcga 117900 cggagtggga gacatctgtgagtctgactt tgaccaggac caggtcatcg atcggatcga 117960 cgtctgccca gagaacgcagaggtcaccct gaccgacttc agggcttacc agaccgtggt 118020 cctggatcct gaaggggatgcccagatcga tcccaactgg gtggtcctga accaggtgag 118080 tgtcacatgg gcggcaatggctcagccttg cctctcaaca gaggcccttc tgatagtggt 118140 aggtcatgtt tagagggtgcagacaataag ccaggcattc ttcacattcg ctcttattcc 118200 tcaacagccc tatgacagactacttcaatt atgcccattt tacagaggat gaaattgtct 118260 gctacagctt tcatgattgtagcagcttta atgatggtag cactgtggta agtagctgag 118320 atgactggaa ttgggacccaggcagtctga ccctagaacc cacacttctg atctctgcag 118380 cagactgcct tctgcatatcctcctcattg tgtaactaag aagtgcaggc acagagaggc 118440 ttagtagatt ttccagagtcacagagcaag tcacattgat tagaagtata tgttgtatgc 118500 tccccagtta agggcctgtccaatcagctt aagtgacagt ctcagcaaac ttatttctat 118560 tcagctttga gcttttttttaacctttaca agcacatgct ttctttgagc acataacaca 118620 atagagcaac tatttcagataatgaaatct ttatatggag gtcagacatc actggccact 118680 cactcatatg gtgcctgaggttttgagctt ccttaaggtt acttggcccc ttcaagactg 118740 ttctgaactc cctcaactctctctgcaggg catggagatt gtacagacca tgaacagtga 118800 tcctggcctg gcagtgggtatgtccagggc ctcagttgcc actcacatag aattcttcca 118860 gctactagtg tggtttgtctattgttctaa tcttatctgg tccctacagg gtacacagct 118920 tttaatggag ttgacttcgaagggaccttc catgtgaata cccagacaga tgatgactat 118980 gcaggcttta tctttggctaccaagatagc tccagcttct acgtggtcat gtggaagcag 119040 acggagcaga catattggcaagccacccca ttccgagcag ttgcagaacc tggcattcag 119100 ctcaaggtat tggtggtttgaagtcattca tctccctttc ttctggacct ctcatctttt 119160 ttcagatatt gtgttttcccatgtggtact tagttaatct aaaaaaaatt gatgcattta 119220 tgctaacgtt agagtcagggaagactacag ttgacccttg ccttcattcc tgtgtgccag 119280 ctacattcta acctctcatggtgggaatcc cagcctacat gaggtcagag agagggaagg 119340 tttagagtgc atcttcaggcacactgtgtt ggaaactggc taataaattt catcgatagg 119400 tccaactttg tacaggagggctgatgggct acttctgtca ctcctgctag gacacaaggc 119460 tgacttaagt agccagacgtttgataggag ccctcattgg gcaaatgtag ggagcaatgg 119520 atgtgcagca ggaacaaacatggaatgtgt aatggaatac tgggcaaggc ttagtagtaa 119580 aaggttgggg tcacctccatagaaatgaca tttaagtgtt tatgctttgt tctgaatcca 119640 aggcagccgg attataaccatgactataac ctggatcatc acttcagatc caggaagcag 119700 gaagggtggt gcctgtgtcctgtcctaaaa ccttgcttgt gtcatcatgc aggctgtgaa 119760 gtctaagaca ggtccaggggagcatctccg gaactccctg tggcacacgg gggacaccag 119820 tgaccaggtc aggctgctgtggaaggactc caggaatgtg ggctggaagg acaaggtgtc 119880 ctaccgctgg ttcctacagcacaggcccca ggtgggctac atcaggtagg tagaggcccc 119940 atctccttac cttgctctagaagcaaagca ttctccgttc tgcatcccag agccttcatt 120000 tccctgagca attctgacctaggatagtcc ccaaggacat gccaggactt ttgtgaccgc 120060 aggatgggga acctagaaaagaggtgggag agaagatatt cagggtcaga ccacccggac 120120 aggacagtag gaccaagatggggatagaag gagcagagct gaggggacga cccagcgttg 120180 tggggttggt gcatttggatggctgtgccc ttctgccatg ctctgcagag aagcctgagg 120240 gtggacatgc caatcggtatgaggtaagag gtgtggcagg gtctcttctc cacaccctcc 120300 cctgtggcac ctctgggccctgtgtaatca cttactagtg cacatttcac cctgactgca 120360 gcactatgtc ccttacagtctgtcttccct gctggatcac aaactgcatg agagcagcgc 120420 ttgtatcttt tttttttttttttttttttt ttggagacgg aatcccactc tgttgccagg 120480 caggagtact gtggcgtgatctcagctcac tgcaacctcc gccttcctgg ttcaagtgat 120540 tcttctgcct cagcctcccgagtagctggg attacaggca cgcgccatca cgccaggcta 120600 atttttgtat ttttagtagagatggggttt caccatgctg gccaggctgg tcttgatctc 120660 ctgacctcgt gatccacccgcctcggcctc ccaaagtgct gggattacag gcatgggcca 120720 ctgtgcctag ccatagcttattattttatc ttgatctccc cagtgcctag cacaaagcct 120780 gacatctact acacgtggcatgaactgatc atcatgtagg ccaaagtagt ctgacttctt 120840 tctttatagg acttttttgttgttgttttt taagtattct cgggagttag gaggcttagg 120900 gctgtgtctt tgaaatgtagatagtgttct tcttcatttg aacttgcaga tcaacaccca 120960 tcctagccta tgtaggatatttaggaagga gtactgagtg cattctcctc atcagggcag 121020 gaaagcagcc ttgtggaaaaaagaaaatca tgagggcaaa ttcatgtttt gtttctcttt 121080 gtgacattat gtcaaatgtactctttttgt ctcacagtga gatgatcatg ttctcagccc 121140 tattaagccc aatgccccttttttattata aatattttga aatgccccct tttccgtccc 121200 aaaatggaat catagaaattacaacctacc tacacgtata atctagaaaa tcaatgtgag 121260 ccctaacaat atcatagagaaataagaata atttataaca aaataattta aatatttgag 121320 cacagccaca ccagaagacaaaatgaaaca gacacttgca cccatgccta gaatcactac 121380 gaaggtgaca ttctaacagactgttgtgtc agtgacttaa atgtttgtta cactggttga 121440 tgacagcaat gtaaatttctcaaatggtac acattttaga atgttaattt tcaaacaaat 121500 tttctcaatt taccctggaaaatatagttt atattaaaat ttgtgcaaaa tatacttgct 121560 gtttacaagt gaaagtgagtcaggttctag gccctgatag ttgctcttta cctaccctgg 121620 tgtctgagga acactccagaagtgtatgga atgcaggaca ggttttgatt gttctcgcca 121680 gggcattgct cgccatccagcatccctggc tgccatcact cagtgccact gtgacaaccc 121740 aaagtgtccc cacagattcctccaactcta cttagggagt gctgtctcct gtggagaatc 121800 attgctctga gattccaccaaaagatataa atcagaccaa aggaaaagcc aaagttttta 121860 agcatggtat ctagacaaggcaactcacat gacaataaga cacaaaactt tctcactcaa 121920 gatagcaggt ttggtttggtttgattttta actcaagttt gatagtgtga ccaacatcat 121980 gttaggcctt gtgagagacagaagttggga agaaacaccc cccacccccc tccacacaca 122040 cacggacaca cacggtccctgccctcaagg acttccagtc tagtaagtaa gtggcaacag 122100 ctacagtaca atccaggtcagcgaaaaatg gagcctaaaa cagagcaaag aaagacgcag 122160 gtacgtataa gaagtggggcccctgggcct cacagtgggc actttacccc gggttggatt 122220 tcatggaggc ccctccggccgtgttgtccg cagggtacga ttttatgaag gctctgagtt 122280 ggtggctgac tctggcgtcaccatagacac cacaatgcgt ggaggccgac ttggcgtttt 122340 ctgcttctct caagaaaacatcatctggtc caacctcaag tatcgctgca atggtaatgt 122400 gcattctcgt tactgttcaacattgttact agaattagtc aaacactgcc accttatttt 122460 tataccgcac ttcccccccaaaagcccaac gctcatacca catagtcatt aaaacctgat 122520 agtccctgtg taccagaagtaaaaagcaga tcacatttat cctattttat ccatgtggaa 122580 attaagaccc atagaatttaactgcatggt ttagggtttg gggttcagtg agaaatccag 122640 tcagatttaa tttacaggaagcctttattt tcatgtggtt aaaacataat cacagatgca 122700 aaatccattc tgcctgctgattttctgcac ggccgggaag gagcactcct gggctttaag 122760 ccaacggttt gcaaagcagcctgcaggaga aaatggcggc gagggcctgc ggggcgtcct 122820 gggcgggcta acagcgccccctacaggacg cctgagccgc gggcgggggt ccggggtccg 122880 gggtggaagg agcctcgctaacctccctgt gcccattcct attgcagaca ccatccctga 122940 ggacttccaa gagtttcaaacccagaattt cgaccgcttc gataattaaa ccaaggaagc 123000 aatctgtaac tgcttttcggaacactaaaa ccatatatat tttaacttca attttcttta 123060 gcttttacca acccaaatatatcaaaacgt tttatgtgaa tgtggcaata aaggagaaga 123120 gatcattttt aaaaaccgtgttgctcagac attgctctca cgtggctcag gctttgaggt 123180 gcccgttctc ggagggatccctgttaactt gcagggtggt agtttcttct cagacccggt 123240 ggccgataag atgagcctatgcgtgaatca caaggcccag gccagaggtc tcagctgggg 123300 tccccccagt ttgctccatacaaaacgcct aaccgttcca tgctcacctt ggcagcctcc 123360 ttatttcggg taaggggtctcagaaacacg tgtttggggt ggcaccttcc ctagggaggc 123420 gtttttctga cctagtcctgggacagattt gcccaattct ttctgatgct gtcatcccat 123480 cccacacctt ccacagacttctattctggc cccttcaacg taattcttaa gcttatttat 123540 atatttggct ccccctagtggactgtaagc atattgacaa taaaggatca tgtcattaca 123600 atcaatatcc ccacagtacctggaatgtag taaatagcca gtaaatgttt actgaattaa 123660 taaacgaaga atgaatgaatgaatgcctct taaaggacag tagagtgtac atgatcataa 123720 aagtcctcct caactcaaaaagtcacaaca tatcctctat ttgtggtacc agtagctgga 123780 tgtacagcac agaaacttgcctaatttgta cactagcctt tcttgtattt caattgtaaa 123840 tcaagatcca agcaaatttttataaattct ctagaaattt tgtttcaaag aatattgctg 123900 tgcggttata tattttcttattccagaatt cctagagata tattaaatca tgtatgaaga 123960 aaaaaacacc acagttttatcatgacttta acagtgagaa aatacaatgg tgcatgcatg 124020 agatctggag gatttctggacatcctcctc tcatgtacag cctggagatg tgtgtggcct 124080 tgtaatccag gacatctgatctcctacatc aaaaactcca atggggccag gtgtggtggc 124140 acttgcctgt ggtcccaagcactttgggag gccaaggcag gcagatcact tgaggccagg 124200 agttcaagac cagcctggccaacgtggcga aacccatctc tactaaaaat acaaaaatta 124260 gccaggtgtg gtgccacttgcctatagtcc cagctatttg ggaggttgaa gtgggagaat 124320 cacttgagct ggggaggcagaggctgcagt gagcagagat tgcaccactg tactccagcc 124380 tgggcaatga gagtgaggctggtgaaagga ggcagcaacc cacaccatgc ccacccctgg 124440 ggcagcctcc ctgttgtcggctgtgcaagc ccaagaacgc agtatgtcca caggaaacag 124500 actctcttgg ttaaatcttttgtgtctata actattttag aaaccatggg cccacaggag 124560 atccagaaca taattccatcccttagggag cttattacat ttttgggaag acaaaacttg 124620 ttatttatgc aaaacaagaaacaaagacgc atgtagaacc agcagtgtgc tgaagccaac 124680 tcctaccagc tcataagagccaactattca agttttagga atattgtgag ccagttaaac 124740 ggttggtagt ttgaaattggccatggtggg agtatttaca cagaaactgg caaacactac 124800 aaatcagggc tccagagaactggttgttaa atatttatca gcacacaagt gattattcta 124860 ccttgtccta ttcctagttggccctcctta tatttttgaa gctgatgtag aaaacttgag 124920 gctttcagaa aatttcttgcataagaaatg aagtaagaaa agtgaaaatg ggtggggtgc 124980 tatggctcac acctgtaatcctagcacttt gggaggccta ggtgggagga tcgcttgagg 125040 tcaggagttt gggaccagtctggccaacat ggtaaaaccc catctctact aaaaatacaa 125100 aaattaggcc aggtgcagtggctcctgcct atactcccag ctcctcaggt ggctgaagca 125160 caagaatctc ttgaacccgggaggcggagg ttgcagtgag ctgagatcgt gccactgcac 125220 tccagcctgg gcgacagagcaagacgtgtc tccaaaaaca aaaacaaaaa aaaaaaaaag 125280 tgaaaatcag gaagcacttgagagaagcta gccagttgca gactgacggg atgagctagc 125340 aggtgtggta tgctctcccccaggtaactc cctgggcttc ttggatactt gttatactca 125400 accttagcac aatgactcaaattcctatta cagttccatt aggttagtaa gagcctcatt 125460 tgaatcttac acctctaggactgagtggcc atcagtctct caccaactag tatgaattca 125520 ttccaatgcc cattcacctcctgacttttc atggagatcg agaagactga ggagctgggc 125580 atggtggtgt gcacctgtggtcctggctac ctgggaggct gaatggggag gatcacttga 125640 gcccaggagt tcaagtccagcatgggcaac atagcaagat cctgtctcac tttttttttt 125700 tttttttaag tactgggggaatgggaggcg tggctgggaa agaaaagagg cattgtcact 125760 gtgggggaat gggaggcgtggctgggaaag aaaagaggca ttgtcactgt agggaaaaga 125820 tcatggctga gtagaatcccacgggaagtg tgggagatct ctgggggcag gatgagggca 125880 ctgggggcag ccagaggaggcctgggggca tgggagcagg tgcacaaagg gatgtgcagc 125940 cctttttgcc tacactcaagaaaaaggaaa aaaaaactct tccttctccc catccaggtt 126000 attagtccta ggctccagaagtattatggt gattatcctt aggttaattt tggagacagg 126060 ctatagagtt aaagagaatgaggaagatat agaatccagc cacctgctat tgaaacatct 126120 gtaggcattt tttattgcctaggctagaag ataactggga acagactcaa gcagcaaaaa 126180 agaatctggt ccagaagtgaggccgtgggc aaactagata ctgtacagca taaatctagg 126240 ggtgagaggt ggggtgcccctgtttgggat acacgcctgg ggtccccagt aacatcggga 126300 ctgtaagggt ggctcaacctttcagtggca tgaagaaacc ccctgcagtg tccacagctc 126360 cgcatcttac agccccgcccaatttggtca taggcaacct caggagacag agacgctcac 126420 cccaaatttc ctggggcagtatagtttcca cagtgggtga gggtgtttta ttccagagaa 126480 ataattctag tactatagaattaagtatag agatgatgaa gtaaattgga cccatcatcc 126540 taatattttt gtgtggtgttggggaattat gtttgaatat gtggcaacgt acttgaaata 126600 tctaagagaa tttggcataattagagaatg caaattgaac ttgtgattcc agtatagaat 126660 gcagacagat gctcttcaccttaaaatggg gctatgtcct aataaatccg taagttgaac 126720 atatcttaag tccaaagtgcatttagtact ccaataaacc caagtcaaaa gtttaagtca 126780 tccatcatga agtccagatactcctcaatt taaatagggt tatgttttga taaaaatcgt 126840 caaaccatca ttagttgggagttgtttata gttgaatatt tggtttatca tcatcatcat 126900 tattagtatt attgagacaggatctcactg tcgctcaggc tgcagtgcag tggtgcagtc 126960 tcagctcact gcagcctcaacctcctggct caaatagtcc ttccacctca gccttccaag 127020 tagctaagac ttacaggctcataccaccat atccagctaa ttttttctat ttttttttgt 127080 aaagatgggg tcttactgtgttgcccaggc tggtctcaaa ctcctgggct caaatgatcc 127140 tcctgccttg gcctcccaaagatagtattt gattttagac ttgtgatttt aagtcagaag 127200 gtaggagaat ttggttgaatatgaaaatat tgagatgatt aagataattt ctcatccatt 127260 atatacattt tttaaatttacaagttatat aagcacatag gtaggtttca tcaaatgaca 127320 aaaaaatgca taaatgaaatttaaatatat tgtttttaat gttacaaatt agcatagtat 127380 gtcataaatt aatatattacatttataaat gggatttaga gtgtttaagg aaatttaatt 127440 aagtttgcaa atgggactaaacattaatca aaagcctaaa agtgattata tattttgcta 127500 tagagtaata agtagatttgcatatttata gaatatagta aagtaatacg atttttaagt 127560 tgaatttttt aacttaaggttttgtaattt gtaactttaa tcagatacta attttaaaac 127620 caaagtcacc ataaattgtgttttctgtaa aagacaacct ttccttcagg aataaggaat 127680 gcctcctaca agctccacattttaccacct cgcccaaatc gttggttaat aacctcagac 127740 aattgacatg caccccaaatttcctgaggc agtagagttt ccttagtgga gaagggtctt 127800 ttattcctga gaaataattctacaattgta gaactaagta cagatatgct gaagtcaatt 127860 ggacccatca tcctaatatttttgtgtggt gcttgggaat tatgtttgta tatgtgccaa 127920 agtacttgaa atatctaagagaatttggca tacagaatgc aataaattca atgtgggatt 127980 ctagtttaaa atgcatacagatgctccctt acttaccatg tagttacatc ctgggataaa 128040 cccacggcaa gtcagaaatatcataagtga aaatgcattt tatatatata tatatatata 128100 tatatatatt tttttttttttttttttttt tagatggagt cttgctctct cgcccaggct 128160 ggagtgcagt ggtgcgatctcggctcactg caagctccgc ctcctggctt cacgccattc 128220 ttctgcctca gcctcccgagtagctgggac cacagacacc caccaccaca cccagctaat 128280 tttttgtatt tttttagtaaagatggggtt tcaccgtgtt agccagcatg gcatttaata 128340 tcttgataag cccatcataaagtcaaaaaa atcttaagtc aaaccattat aagttgggga 128400 ttgtctgtag ttgagtatttgatttcagac ttctgatttt aaatcagaag gtagggagaa 128460 tttggttgaa tatgtaaatgatactgaggt ggttcagata atttataatc cactgtataa 128520 atttttttaa tttacaagggttatttaagt gcgtaggcag gttttgtcag acaagtgaca 128580 taaaaataaa acattataaatgcaatttaa atttagttta taatgtatta caaattagca 128640 tagtatattg ttataaatatattaaattta taaatggaat ttagagcatt taaagaaatt 128700 taattaagtt tacaaatggcactagacaat caaaagctta caagtgatta taaattttgc 128760 tataaagtaa taagtagatttgtatattta tagatgtagt agagtcgtaa gatttttaag 128820 ttgaacttct atacttaaggttttgtaatt tgtaacttta atcaaacatt aattttaaaa 128880 accagtcacc ataaattgtgttttctgtgt atgaaagcta cccttataag caaataacaa 128940 aatcaataaa aaacagcacagtgacagtca gcactgattt tttttttatt gaggggtgga 129000 gatctggaga actgcatcctcaattccaaa taaggggcta acaatcatcc cagaacactg 129060 cccttttcta cattggggactgtcaccgca cagagggatt aaggaccttg attcagctct 129120 ccctgactcc agggcatcaccaagcagctg gctgcagcaa cgctgggggc ctgagctatc 129180 ctgctgtcag ctctcaaacccctcattttc caggacacat ggcctgaaga atgcactagc 129240 cccaacttgg ggccctggtgtggcgatgtg gcaaggagca cctctctctt ctccctacta 129300 catcaaaaat tttaaggatgcctaaacatg tctgcttatc tggagggctg gggtgaatga 129360 gacgtccaga atgaaagctctcctattctg actgcgccac ctgactgaga agtaacttta 129420 gcttatttcc ttggctcatcataaaagaac gaaaagtatt ttcctcacat tgtctacctc 129480 ctcaactatg gagtggacatgcatccacgc cttcaactca ggtctttgag aaaaacaact 129540 ttatttccca aatttcaccttgtttttctt ctgagaactt attgtgccaa actcaagaga 129600 aacataacct cagtctttcttggggttccc attcttcttc cagaatcaag actctaggat 129660 gtcttctcag aaccaaggtgtttggggagg gggacatcta tccacacaga ttccttctaa 129720 cgtgaccata gtcagcacccaccccccata ctctaacaga ccacactggg gacctcaaat 129780 ctgtgtgaac ctttgcagccattcctttac tggcccctta cctatggact gcagaaagct 129840 cccacaggcc tcccttccttctctctcagc atgggagaat ccttctcaag tatgaggagg 129900 gaagccttat tctcccttttgcttttggcc aagaaatctt atccattccc ggtgttcctt 129960 cacttacgcc ctgctatgacttgaatgtgc cccctgaagt tcatgtgttg gaaacttaat 130020 ccctaataca acacttttgaaaattaaggc ctagtgggag gtgtttaatt catgagggca 130080 gagcccttgt gaatggattaatgtcagtta taaaagacct tgaggctgtg agttccattt 130140 cttgctctct tatactctctgcccctccat cttccaccag gggatgacac agcacaaagg 130200 tctttaccag ataccagcacttgatctatc ttggacttcc caacctccca tgtaaaataa 130260 atttcctttc tttgtaagtcaccccatcta tggtattctg ttatagcaat acaaaatgga 130320 ctaagacact cccacaaaccccagacttcg actatgggct tagctctggg tacacaaaaa 130380 ctagaacagt ttgcaagctggctctgactc acaccaccac agcctcactg aaatagtggg 130440 cccagagtcc actctgccagaatgggagga ctggggcaaa gggaaatatg aaaaggaaat 130500 gggtcaatat ctcaaagtgctaccacaaca tattgtgaac aacttcaata gctaatttta 130560 aaaaattctt ttttagaaatgaggtctcac tatgttaccc aggctggtct cacactcctg 130620 ggctcaagca atcctcccacctcagcctcc caaagtgctg ggattacagg catgagccac 130680 cacacgtggc ctcctactgtttagatttgt tcctaaaaga tgcctatgtt taaaccaggc 130740 cagatcactc accctagaaattccacaaat aaccaatgag ctgatggctc caggcagacg 130800 ggtggctgag cccagttcccatcctggcct ggaggaggag acctaggaag tcacagctta 130860 ggatacactg gggcgggcgggaaggtttgc ttttatccat catccaagct gtgattcagc 130920 gcataagtct ccctttcattaagtttaact tgttgagtaa cattaaattt atatactgca 130980 aagcacaata gaatttcactgctgtgaaca tacgcgttca caagcatcat ccaactaagt 131040 gtgagcatct gctgcctcattgggggtatt tactagtcat tcattattta tttatttatt 131100 tatttttgag acggaaccttgctctgtcgc ccaggctgga gtgcagtggt gtgatctcgg 131160 ctcactgcaa gctccgccccccgggttcac accattctcc tgtctcagcc tcccgagtag 131220 ctgggacgac gggcactcgccaccactccc ggctattttt ttttgtattt ttagtagaga 131280 cgaggtttca ccgtgttagccaggatggtc tcgatctcct gaccttgtga tccgcccatc 131340 tcggcctccc aaagtgctgggattacaggc gtgagccacc gcgcccggcc tagttattta 131400 ttttgcagca cctattagagtaaacagtag cagatatcta atgtctaaga aaaatttatg 131460 gaaggaagat ttgaacgaaaaattttctcc attagcatat ttttaaggag ttttcctcac 131520 aacaattcca tcccactcctacccctccta ccagaattct gaggatcagg gcttgagaac 131580 taacatctcc aaagtcccagtagctctgtg gttttctaca ggctcctgga ggataaggac 131640 cgtgccatgt ttatctcaccctccacctct caataaagta caaatttgtg gaattggcga 131700 ggccaagagg cctcaaattcttgtatcttt catatcatcc actagggggc agcaatcctc 131760 taaaaatagc ccctaaaccagccacttttt tagctaaaag atgactaaat ttcattaaaa 131820 gtagaataac aaacgcctgtaatcccagca ctttggcagg ccgaggcggg cggatcacga 131880 ggtccggaga ccgagaccatcctggccaac acggtgaaac cccatcccta ctaaaaatac 131940 aaaaattagc tgggtgcggtggcttgcgcc tgtagtccca gctactctgg aggctgaggc 132000 aggagaatcg cttgaacccggggagcggag gttgcagtga gctgagaccg cgccactgca 132060 ctccagcctg ggcaacagagcaagtatgtg tctcaggaaa aaaaaaaaaa aaaaaaagaa 132120 taacaacaac tacaatttattaaacactta ctctgctcct agcactatgt tatatgctaa 132180 tagttcaggg cttaagaacaattttattgg ccaggagtgg tgtctcatgc ctgaaatctt 132240 accactttgg gaaggtgaggcaggaggatc gcctcagctc aggaatttga gaccagcctg 132300 ggcaacatag tgagacctatctctactgag aatgttttaa taattcgtca ggcatggtgg 132360 tgtgcactgt agtcccagctacttgggagg ctgagatggg aggattactt gagcctgagt 132420 ggacaggctg cagtgagctatgattgcacc cctgcccttc agcctgggtg acagagcaag 132480 accctgtcta aaaaaaaagaactcttattt atcaaaacga acatctcaac aaaaagcaac 132540 aaccaccaca taggaaggaaaccaccacct aaacgtgaat tattgcaact tagtctctga 132600 acgaacactg aagcctcgctttcttttagg tattgcttcg caaagacttc tgccttgtcc 132660 ctcagcctaa attcacaggatagagtcttc tccatgagca aagcaaagag ccattcatct 132720 atccactcat cattcactcattcttcagac acacactgag caattactac ataccaggca 132780 ctgtgctaga tactgggagggattttaaaa gtacacaaag ccagtccatt ttagtccagg 132840 cctctgtatg actgaaagcctttgtcttac tattaataat actgatcata actgcaaaat 132900 tatagtgtag tttagaattaaatgaacctg gccgggcgca gtggctcaag cctgtgatcc 132960 cagcacttta ggaggtggaggcgggcagat cacttgaggt taggagtttg agaccagcct 133020 gggcaacatg gcaaaacccatctctactaa aaatacaaaa attagccacg tgtggtggca 133080 tgcacctgta gtcccagctacttgggaagc tgagacagga gaatcacttg aaccttggat 133140 gcagaggttg cagtaagattgtgccactgc actccagcct gggcagcaga gggggattcc 133200 atctcaaaaa aaaaagaattaaatgcacct caaagccaac cactagattc tctcgctctg 133260 tttagaagac atttgtttgttgtaagaacc cagacttaat cctgagaggt tttagcgggg 133320 tctaaagaga gcctcgtcctaaacatccct gccaggaaga acacttccag ggagcagggg 133380 gcaggcagct tcctggcccaggacagtctg catgacctaa attacttgag tcaaaaattc 133440 tttgggccat atagtgttttggtggttgct gttgggtttt ctatttgcta acttgagcaa 133500 acatttaaaa tttgagtgtttttagacaaa aacctggatt tccaacttct ctttaaaaag 133560 tcaaaagctc cagcctccatctgcattcac gcacaggaac cttgaaaagg gcctgtgttg 133620 tccatgcacc gcagtgtccacacatgtaag tttctacctg actctgtaag tgatcgagta 133680 tgagagtccg tacttgggatttcccaggta ccctgtgcgg cctgcagggg atatggagaa 133740 ggttgggact caacccctcttgtggcaggc caggtctcac gaatagctaa acaggcaggc 133800 ctccatgaca actgtttcagcactgactga gtgcttaggt taaacattaa aggctgcaag 133860 agccagtgtc cttacacaaaggctggaatg tgacaaaagc caccaagagt cttgcctagg 133920 cctttcctgg gccttgaagcatgacaagat aacaaagaaa ttcttacatt cctcgtaccc 133980 acgtttagga ttaaacacgttttattaggg gtcagcaaag aactccccag accgccataa 134040 ctcagcggga ggcaagataagggtaatcac ccccagcacc tggacccatc tagattaaat 134100 aaacttactg aggctccggaggaaggtctc caggactcag atcttagtta tggattacag 134160 ggaatcagtc acttgtgtcttttgatgaat ccacacttac atgtagacat atagcttcat 134220 ataagcactg gaaaactttgtaaatatgag tcggtctggt gatattttcc tggccttctg 134280 cctgtaccgg gttacagaaataaactctct tctttcccag tttatctgca tctcattact 134340 gggccatgag aacaagcagcccaaccctca gttcagtctg ggaacactct ggctaacaac 134400 ctgttagggg ttgtcaccactgcctggaga ggataggggt acactggggg ccagccgaca 134460 ggagctgagg tctcaggtagaagattccta ccttatcaat agatggaaag agattcaaac 134520 ccagcatcac tgactctggttccaaaatct gaaattcttt tatagcgaca gcacacattc 134580 accaggtatg cagagaagagttaacagcag gtccgagatg gctgctgatc tttagttaga 134640 aaggcctaat tgcaaagttggcccttggct ggtatctgag cacttggctt tccagagtgt 134700 tcccaccatt tctgaaactgataagagtgg ttcaccatgt cttgactgta cagtgtggtt 134760 tatgctgagc atctgctttctttctgggag ttggaatttt ggtacatggt agggagaagg 134820 tgcctacaag acgagaccctaataaaaacc ctgggtgttg agtctctaat gagcttccct 134880 tgtggccaac atttcatatcccttgtcaca actcattgct ggggaaatta agcacatatt 134940 atgtgacttc cacctggagaagactcttgg tagcttgccc ccctggtttt ctctggactt 135000 ggccccatac acttttctttcttttttttt tttggtgctg attttgccct gtatcctttc 135060 actgtaataa attataactgtaagaataac tagatgctga gttatgcttc agccaatcac 135120 tagattctat ttagagggcatttgtttatt tgaagaactc agacttactt aatcctgaga 135180 gtttttacct gggtccaaagagagccttgt tctaaacatc cctgtctaga aagcctccga 135240 tgagactttc tagcaaaccacttaaatgta gtggtcttgg agaccccgaa acatcagtca 135300 actatctcca acaactgaaagaggctttgg gcttacaaag tgcaaagaat aattgaggtg 135360 ctaggtcatc acaggcatcactaatgagaa aataatggaa aacaggggcc taaaagttga 135420 ggccaatcac cacaaaacacctgcagagct ggagaagcaa aactcaaagt ggagacccct 135480 gagattggac acatgacaagggacagagtc taactgaagt ggtttatttg gttagtctca 135540 tacctagaga aatttaaggtgaggcagtca cccccaatac cagctctacc cttctttcat 135600 agtcatagtg tcccaaatttttgcaggaac ccagatgccc agaataaaag attatacttc 135660 ccaggtgagt tgtggccatctaagttctga tcagtggcat ataagcaaaa aagttcatgg 135720 tagcttctgg gaatcttcttctaaagaggg ttaatttttt tttttttttt taaatggagt 135780 ctcactctgt tgccaggctggggtgcagtg gtgcaatctt agctcactgc aacctccgcc 135840 tcccgggttc aagtgattctcctgcctcag cctcccaagt agctgggatt acaggcatgc 135900 accaccacac ccggctaggtttcaccatgt tggccaggat ggtctcggtc tcttgacctc 135960 atgatctacc tgcctcggcctccgaaagtg ctgggattac aagcgtgagt caccacgccc 136020 agccagatca ttaatatttcttattcgtcc tcttatccct cctcctaggc tggaatatgg 136080 atataatagc tggagttccagaggctgtct tgagttgatg agaaagcctt gggaatagaa 136140 gcgtcatcac agagaacaagatagaactga caccgtggag taacatacca gtaacagacc 136200 ataagatttc tatcactggactttgtggac atgaaaaatc tccatctgtt cttggggctt 136260 tactgttact tccagctaaatttaatcctc cttaatgcaa aagaaattcc ggatggggaa 136320 gtgtttataa agtcatgtatccagaaccat agactcacca aatcttagat ctgaagagtc 136380 atcaggaagc tttttgtttcagccttttca ttttacagtt ggaaaaactg aggcctagtc 136440 acctctgtgg tttgtcagggttacatggct agttaggagc aaaactgagg ccagatccca 136500 ggttttgggg ctacaggctcatggttctca ttttcctgct atgagaactg cctcaggtag 136560 gccagggcag gggtaaagtccaaaaggggg tttctgtcta gaggactcaa agaaaaagca 136620 gtgtcggatt gccgtagctcacgcctgtaa ccccagcact ttgggaggtc gaggcaggtg 136680 gatcgcttga gcccaggagtttgagaccag cctgggcaac atggtaaaac tccatttctt 136740 caaaaaatac aaaaattagctggatgtgat ggtatgtgcc tgtcgtccaa gctacttggg 136800 agcctgaggt gggaggatagcttaagccct ggaggttgag gctgcagtga gctacgatgg 136860 taccactgca ctctagcctgggtgacagag tgagactcta tctcaaaaaa aaaaaaaaga 136920 aaagaaaaga aaagaaaaaaaagaaaacat gctgatagac tgtacgtgat gaaatttcag 136980 agatctcctg ggaggccatcccgcttccag tttcgatggg cagagactca acagtggaag 137040 gcaggtatga cctgggacttctcaggcccc agcttccctc ctcttcacct tcctctcacc 137100 catcagactt cacacctccctcttcccgtg gtgttcaaac ctaagtctat acagagtgcc 137160 tcgaacccat atggtgcttagtggtggaac ccgggctaga tttcacccca cctgtgcctt 137220 cttggccagt ccctctatgaagtacacaga ccgacaacct tttacagagg actgggcttg 137280 acaaagagct cgcttggatccatcttgctt ccctgtgcgc acccggcccc actcacaccg 137340 agggagtttc ggccctgctggaaacaccgt ggcagatgct ttggtggcct gcccatttct 137400 ttcactttgt cattcacggatgtcaaagca ccagcacctg cagttctttg tttctgggct 137460 tcctctggtg caaaagctgctttttctgcc tatgttggag gtgcggggga tttaggagac 137520 ctaaggaatg ggctccagtgagatgaaggg aacgggtgtg taaagcctca gcccctcgcc 137580 tggtgggggc tgagtctgaggaatgtgccc tgcattgttc ccgcatgatt aggctccagc 137640 tgcccccagt ggtaaggcccctctcctccc tctcctccct ccccagtcta ggggtgcttc 137700 acctcccaaa taaagagggttcactcatcc ttatctcagg gtctgcttct gggggagctg 137760 accagaagca aaccttcctcagcactcaga ttttacttac tccaaaatta gggaatatta 137820 agtttgaaca gataccctgtgctcaaaagc atgcgcacag acatatatat agaaatttcg 137880 ggccgggtgc agtggctcacgcctgtaatc ccagcacttt gggaggccaa ggcggatcac 137940 ttgaggccag gagtttgagatcagcctggc caacctaggg agaccccatc tctacaaaaa 138000 taattattta gctgagtgtgatggtggaca cctgtagtcc cagctactcg ggagactgag 138060 gtgggatgat cacttgagcctagtagttca agactacagt gagccatgat ggcaccaccg 138120 tactccagcc tgggtgacagagtgagaccg tatctcaatt aaaaaaggaa atttccaact 138180 tttctctgag cccactacccaaagacaatc atatgcctca gccactcagc tgagctttac 138240 tgcccttcca agaatagcctagaaatccag taaggacaat ggaaactaga aaaatgaaga 138300 gacactgata ctggatctaattcatatttt taaccccact caccacctgc aatcacaccc 138360 acccacagcc ttcagtaagtgctcagtaaa tatctgttag tcagcaaagc tactgggagc 138420 acacactctg cccctgcgccatacaccctg cccccaaccc ctgctctatt atggtgattt 138480 ccccactcct agtctctctgcacagaatgg tgaagaaact gcatttggtt ttttcaaact 138540 attcttcagg ccgggcacagtggctcatgc ctgtaatctc agcactttgg gaggctgagg 138600 tgggcagatc acaaagtcaggagttccaga ccagcttggc caacatggtg aaacctcgtc 138660 tctactaaaa attaaaaaaattagccaggc atggtggtgc gcgtctgtat tcccagctac 138720 tcaggaggct gaggcaggagaaacgcttga acctgggagg cggaggttgc ggtgagccga 138780 gatcacgcca ttgccctccagcctgggcga cagagcaaga ctctgtctca aaagaaaaaa 138840 agaaaaaaaa aatacaaaaaaaaaacctat tcttctgcac cctcaggaga atgggccagg 138900 aacatgctct tgaaacaggctgcttgctca gaaatacatt tccatattag caggcgcagc 138960 tgtcactcct gctggaggcctgttggcttc ttgagaactg ctagtctcac catttgccac 139020 atgcccaaca ggaaaatttaattcacagaa actcagaatg ccagattcct cctccctccc 139080 aactcaggga tcccctcaagagcctgcctg acagctgtta tctccttcat gaacacctca 139140 agtgacaggg aactcaccgctgtatgatgt agcctatcct attgttggaa ggctctagtt 139200 actaataatt cttattagtaaacatcatct attaaacatt tgctatgtgc cagcctctat 139260 taaaagtaat ttaactagccaggtgtggtg gtgtgcactt gtggtcccaa ctacttggaa 139320 ggctgaggca agaggattccttgagcccag gagttcaaga ctgcagtgag ctatgatcgc 139380 gccactctac ttcagcctgggccacagaac aagacctcaa ctcctttaaa aataaaaatt 139440 aaatgaaatg tgcttaacctgtgtcaactc atttaatcat gacaacaatc ctatgaaata 139500 gatgctattg ttattactccacctgacctg acaggcaagg aaacttacag cacagagaag 139560 taaataatta gctgaagatcacagagctag ctagcaagtg gcagaatgca atttcaaaca 139620 aaaagtggcc tttgagagctttcctctgtt gccttttttc tacttggggc agaaatcttc 139680 catcctataa gtgagcctttttaggtccag atccttgtga aattgtgatg agtgccaagg 139740 accctttcca tgctactgtgcatatggtca caaaactctg catattattt cagggagtca 139800 atctacccca gtccttggaccccaggttaa gaaccacagc taacacaaaa taacgttgct 139860 cttgtatcta tttggaaattctcttatgaa cttttttatt tctaacagac tttctccaag 139920 cttaattttc cctatttttttatttttttc agacagagtc ttcctctgtt gcccaggctg 139980 gcatgcaatg gtacaatcttggctcactgc aacctctgcc ttctgggttc aagtgatttt 140040 cctgccttag cctcccaagtagctgggatt acagatatgc accaccacgt ccagctaatt 140100 tttgtatttt tagtagagatggggtttcac cgtgttgtcc aggctggtct cgaactcctg 140160 acctcaagag atccacccatctcagccttc caaagtgctg ggattacagg catgaaccgt 140220 cgcgcccggc caattttcccagttctagat gaaagaatgt tcaaccacac caatactcaa 140280 agatagacaa attcaaataataaggttaag gtgttatttt gtacctttat aacaacaaac 140340 aactataaag aaaatactcaaaaatcaagt gctggccagg tgcagtggca gacacctatc 140400 atcccagcac tttgcaaggccaaggcaggt ggatctctga gctcaggagt tcaggaccag 140460 cctgggcaac aagatgaaaccccatttcta caaaaaatta gccaggcacg gtagtgcgcc 140520 tgtagtcgca gctactcaggagactgaggt gggaggatgc cttaagcctg gaaggccaaa 140580 gctgcagtga gctatgatcacaccactgca ctccagccca ggcaacagag caagaccctg 140640 tctccaaaaa aagagtgctgggagggttgt actcctgttg ctgctgctat ggtacctgta 140700 tacactacca ttagaaatttggcaacacac aggaaagact gatgaaaaca cccataagct 140760 tttgacccgg taattctactcctcaaattt attccaaaaa aataatttag atggaaataa 140820 aagtcacatg tagaaagatatttatagcaa acttttctct aagagccaga aaaaaaaaaa 140880 aacaatgtcc aaaaaaacaactgggaaatt ataagcaaat atggtcatta gcttaatgga 140940 acatataaaa taatgaaaagcattaaaata acatttagga aaaaatgtgg aaggtggaaa 141000 tcaacatgat gatgattgtcaattgtgggg tgatagggtg gggatgccca gggaactttc 141060 aattcgcttt ttattgcctcactttaaaaa gtgagagacc aaataatcag aacaaaagca 141120 gctcagagga aagagagagaaactataaag gatcagagga tccctcaaac caaaatgaaa 141180 caaaacacta taaataatatcctctatgaa atatgaaaag atatgcatcc acgaaagaga 141240 aacagaagag gatggggtgtaatcagaaaa catgaaagaa ggcctggcat ggtggctcac 141300 acttgtaatc tcagcactttgggaggctga gtggggcgga tcccgtgagg tcaggagttc 141360 gagatcagcc tggccaacatggtgaaatcc catctctaca aaaaatacaa aaattagctg 141420 ggtgtggtgg tgcacacctgtaatcccagc tactgggagg ctgaggcagg agaattcctt 141480 gaacccggga ggcagaggttgcagtgagct gagatcgtgc cactgaactc cagcctggga 141540 aacagagcaa gactcccatctcaaaaaaaa aaaaaaaaaa aaaagataga gttattggag 141600 atggagttat aacaactgaagtagtaaatt caacagaaac tctggaagat aaagtaggct 141660 aactatccag aaaattgaacaaaaataaat agagagatag aaaatgggag aaaagatgac 141720 caatccagaa gacccaatatctacctaata ggagtccgct aagagacaac agagacaatc 141780 tccaggacag agagtgtaactggccaagcc aaaggcatgt gcccaaccca tggctaggta 141840 ggaaagggag gatctggcccatttgggtct agaaggaaag ggcactgtct ccccccacaa 141900 cccaggagta tggtcccccaaaaggaaaag taatagatgc tactagaaaa tgagggttag 141960 atgatataca gccaagagctaccactgtcc actgcacagg aacctctgtt gactcctagt 142020 aaccacacct tcttctctaaatactcatag agcactttca ataggatttt tgcatcctgt 142080 caaatgcaaa acctccctctctaggcctgt tgtgcattat catagctgag atgtgtacat 142140 cctacatgcc aattgtttctcatgagttat ctcattaaag ccttacaaca aagcaaacaa 142200 gaaaatcatc ttaataaccccaattataaa tcaggacact actgcccaga gcggttaaga 142260 agcatgccca gcttcacccagctaataaag agattcaagc caggatttgg acctagtacc 142320 ctgaccatgg agctcgcactcttatgccac aggactttat gtacctctcc ctggaggctt 142380 ctcggtgcct ccagtgaccccgatgcctca ctgccttctg gaccccaaag aggatctaaa 142440 gtaggtcgtc aaacagccgtgagagcctcc attgagccac acaccccgag ttttctgcat 142500 gcagcccagc acctagctcagagagtccct ggcatgcttg gtcaccctag gaggtccatt 142560 agccctccca agtcaccagggtgctgggag ccgccactag atctcagccc tccttcacgt 142620 cacacacctc actcaggggctgtccacagg cttgctctgg ctgtagcatg ggaacccctg 142680 ctccttctta tctggtgcaaggagcccagg ggatgccttt aaggaaggtc agccctgtcg 142740 gtgagacatg ttgtgcaaaaggcaggttga ctggagaaaa gtcatacacg tttatttcac 142800 atttatacac aggatccttcagaatgaaaa cccaaagata acgcggaaat tggccatact 142860 gatgcttaag attaacgaagtatgaacagc tatgtacaaa tgcgactgga caaaaaaagg 142920 gtatgatcta atgctgatagactgagctgg gagattcagt gaggcctgtg tagattcttg 142980 gcctctctgc acagcatccctttcttctgt gtgtggggca ggaccctctc gggaatgggg 143040 gaattatgac ctacagtcaaacaaggtaag tcggatcatt tctttatggc cagtttttac 143100 acagaaaggc agagggaaaataagagtaat atttttagga tttatggctg gctttgggga 143160 aaagaggttc tggtttctatggggaagagg gattctagtt tctaaggcta gactagggga 143220 gaacgcgcct gagagaaaggagggcaggta tgcagagaaa aacttttaac ttctgaggct 143280 ttcattttag agtactcttttctgagtccc aacagtggga agccagagga agcagaactg 143340 tacttgtgag agcccccagacagctggctc ttcccccacg cagcagcagt caggccccag 143400 agctcctctt gcacgacagcacaagggcaa agcctgtctg ggctggtgga agcttccgtg 143460 ggtctggctg tattggggggaagacctgtc tgggagcctc ttcaactatt tatctgttct 143520 gccttcctcc cccttgctcctgtcctttga acgctaacct ggacacagaa gctctcagca 143580 tttaggaggc cttaattgcagctccattta atgtaagatc tcttgaagtg ggtcccaatc 143640 ttaggccaca agttcaggtgtacagaaaac aaaccatttc tctttgtctt tttttttttt 143700 tgagatggag tcttgctctgtcacccaggc tggagtgcag tggcgcaatc tccacctccc 143760 gggttcaagc aattcttgtgcctcagcctc ccgtgtagct gggattacag gtgtgcacca 143820 ctacgcctgg ataatttttgtatttttagt ggagacaagg tttcaccatg ttgccaggct 143880 tgtctccaac tcctgacctcaggtgatcca cccgcctcag cctcccaaag tgttgggatt 143940 acaggtgtga gccaccacacccagccaaca aacaattttt ctgtcacaga gtttaattct 144000 atttgattcc tatcctgccaatgtggcaga aacataatct ctgttgattt aaaatttacc 144060 cacatcctga acctgggatcttgtcaaagt cccactgagc tctgggagat ggggtcttta 144120 ttgtcttttg tcacacaggtgtctagaaga tgccacctat tgtcacttac tttgtgtctt 144180 acacaactgc agaccactattcaaaggcta tatagtttgc tctcactgct gtaacaaatt 144240 atcacaaata taatggcttaacacaacaca aacttatttc tcacagtttc tgtagaagtg 144300 caacatgggt cttaccaggtggtggctgtg ctgggcttat agctggagac tctgggaaag 144360 aatcctcttc caagctcgttcaggttgctg gcccagttca gttctttgtg ggtaaacttg 144420 ctaggtggca gctagggtcaccctcagctc cttaggttcc ttctttggtc cttgcacatc 144480 atccaggagg catcagatctttctcacgct tggaagatca tggactcggc cttctgcggc 144540 atctgactct tccgccttccttttctgctt gattacatcg ggcccacctg gatgtgctgg 144600 gtcgcctccc tattttaaggtcagcttatt agtaacctta attccacctg caaagcccct 144660 tcccagcaga acctaaatttgtgtttgatt gaccaaacag ggagaaggga gagtatcttt 144720 agagttttgc ctacagcaaaggccactgtg tgagcacatc cctgaaggtg tgcagggcag 144780 ctgccatcac tcacctctcagcccattcac tagacagatg ccctgtcaag gtcatggtcc 144840 ttttaaggct gaaggtttgctgcccctact gcggctgtct tgggaaagaa ctggttctgt 144900 tctgaaccat gctgggatagaacctctaga aggcaaggtc ccgtctacct caacacacac 144960 acaagccatt tcctctctggggtctcccag ggctctttct aggttgcagg aaacaggacg 145020 atctgccccc tgtttccgacctgtaccccc acctctgcac tgtcattgct ttccttcttc 145080 cccacaccac caaggaaatcaccttctcat caaacctccc atatgccaaa tattttggct 145140 agatctggag tcctgtacactctggagtgg ctttgggctt tttgaatccc aggtccagtc 145200 ctgctttcct gtctgtcacattccacagct gaggcaatga gccaggaagt cacctgcttg 145260 gaagttggag ggggacagggagttggagaa tggcaaagtg gggttaatac tgcaaacctg 145320 ttttcctgcc actctcctgacatgctctgt tctttaaaat gcccttggca cctggtccat 145380 cacaggccgc atccttagcaactaagttgg aagaaatggg gctctttggc cagcaatgtc 145440 ttcccgccat gggctatacctgcaggtgcc gggtgaggag gactgcgggc agctctgaag 145500 cctccacttt tccttatgggttttttgttc actctccagg gcctaattca gatgtcacct 145560 cccctgtgcg gcttctccaacccttcagat agatgtgatc actccctccc ctgagcccca 145620 gaacactttg tccaatttcctgcaccctct gagagcgtgg cacaggcccc ctccactgtg 145680 taacaactca cagcccctccctgcatcaga agcccacccc cttcctccgg ggtgcctctt 145740 ctttctagtc atagtgtcttccctcagtag tctatccctc tggggactct gaatatcttc 145800 ccttccccct cagcaatccctagcacactt gcagctgtga gggctgcacc tgaggacaga 145860 gcaggggcgg gaaccccagtcttagaggct gttgtcatca aagttcaaat ggaagatcct 145920 ctgagaagga tctgcagggagaccaattgc cacccctagg gaaggccggg cttctgtggt 145980 atctcaagcc ctgggacctagaggcagctt cccgtaggga gcagccagaa ggccctttcc 146040 ctaccccctc accacccagagaggcctccc tgggatgccc tcccctgaac cctagagctt 146100 catggggatt gctatgtttgcactgtgatg tcatttcaag gtcatggtga tatgcgctac 146160 agctgagcaa gattgttcccagcatcagat tggaagttga aagaaaggct aagtcacaag 146220 aacccaagag ctgggtgaatgcacagctgt gccctaggcc caccagcaag gaccacattt 146280 attaggctca gcctcccacacaaacggtgc cagaaagtct aagagaagaa ggggaacaga 146340 ggaagggagg gaaagtcctttaaatcaaca tggtttcatt gagcttctgc accatgcagg 146400 ggcctgtggg attgagaaggagaatataat cctagtcctt gctctcagga agcttatcat 146460 ctgtgtagag cccaagagtgagcagctggg tgaacaagaa gtggtgcaag ggggaagagt 146520 taccacagat ggtataacagtttgctcagg ctgcctgaaa caacagaaag ttactgtctt 146580 tacagttctg gagaactggcctaggaaatc aagatggcgg cagagctggt ttcttctgag 146640 ggttgtgagg ggtctgctccaggcctccct cggcttgtgg atgtccatct tctccctgtg 146700 tctctttaca tcatcttccctctatgcatg tctgtctttt cgcccacata tccccctttt 146760 aaggacacca gtcatatcggatgagggccc atcctaatga cctcatttta acttttttac 146820 ctctataatg accctgttttcaaatataat cacactctga catactaggg gttagggctt 146880 gacatggttt ggctgtgtcctcacccaaat ttcatcttga gttgtagctc ctgtaattcc 146940 cacatgtcat gggaggaacccagtgggagg ttgttgaatc agggtgggtg agtccttcct 147000 gtgctgttct cgtgatagtgaataaatctc atgagatccg atggttttat aaatgggagt 147060 tcccctgcac acattttctgtcttgcctgc tgccatgtaa gacgttcctt tcaccttcca 147120 ccaagattgt gaggcctccccagccacgtg gaactgtgag tccattaaac ctctttttct 147180 ttataagtaa cctagtctggggtatgtctt tatcagcagt gtgaaaatga actaatacag 147240 ggctccaaca taccttttttagagggacac aattcaaccc acaacagtgg ctttggggag 147300 aaaggaagac ttgaaagaggagaatttcct cattcatcta tctatctatc tatttatcta 147360 tctatctatc tatttatttatttttgagat ggagtcttac tcttgtcgcc agactggagt 147420 gcattggtgc catctcgacttactgcaacc tccacctcct gggttcaagt gattctccta 147480 cctcagcctc ccaagtagctgggattacag gcacccacca ccaggccggc taatttttgt 147540 atttttagta gagacagggttttaccatgt tggccaggct ggccttgaac tcctgacctc 147600 aggtgatccg cccacctctgcctcccaaag tgctgggatt acaggtatga gccaccacac 147660 ccggccagaa tttcctcatctaaaagggca ggttcccagg cagaggtaaa cactgggagg 147720 ttttatactt tgtgattactgaataattcc tcaaggatgt ttttctagat ttgaggaaca 147780 tcttcaggga cagaagatgccacattgggc agggcttctt catgcaggcc ccaaccacag 147840 gggcaacaga atcctggtccctgggagact gtgcccagcc atgagcatca gctgaagcca 147900 caacctcaaa cattcctaccaaaagggaag ctagctgctg ggatccacat gagggcaaag 147960 tggagatgaa ttcagagggcaagaagcagc cagtagggca agagggagag aggacctttc 148020 ttcctgaccc accccacacatacaatggac taaatatttg tgtcccctta aaattcatga 148080 gttgaaatcc taaaccccaaagtgatggca taaggaggtg ggaattttga agggtaattc 148140 agtcatgagg gcagagccgtcatgattagg atttagtgac cacagagagc tagctcaccc 148200 actctaccat gataggacacagcaagaagg ttccatctat gaaccagaaa ctgggcctgg 148260 ccagacactg aatcttcctgcaccttgatc ttggacttcc cagactccag aactgtgaga 148320 aataaatttc tgttgtttctaagacaccag tgtatggtat ttttgtttta gcagcccaga 148380 cagactaaga caacacacaaacaggaaaaa tcagatacct cctgctgcct agctagatgg 148440 cttaagagtc caggacatagacagaggtag gggcaagaag gctgctgggc ctgggcttta 148500 cgttcaagaa aggacttttagacttttgat gttatttcca actgatgttt gttatgggcc 148560 aagttgtgtc cctccaaaattcatatgttg aagtcctaac ccccagcaac tcagaatctg 148620 actgaatttg gaggatagggttaagacgtc agtcagttaa aaagaggttg tgagggtagg 148680 tcttaatcca ataagggtgtctctataaga agaggaaaat tggcacagac acacacagag 148740 agaagaccac atgaacacacaaggagaaga tggcatctgc aagccaagga gacaggcctc 148800 agaaaccaac cctgccaacaccttgatccc tgacttccag cctccaggac tgcaagacaa 148860 taaatgtctg tttaagccactggtcctgca gtagtttgtt atggcagccc tagcaaacta 148920 gtataaagtt ctatccatatgcccctggga tgtgtttcta agcattcatt tttaaaatgt 148980 aacatttctt aaaaatatactgtaacattt taacttgttt tggcatttct gctttttttt 149040 tttttcatgg gacagtcttgctcgctcgcc caggctggag tgcggtggca caatcttggc 149100 tctctgcctc cggggttcaagcgattctcc tgcctaagcc tcctgagtag tggggattac 149160 aggcgtgcac caccaggcccggctaattta tatatatata tatattttta gcagagacag 149220 gtttttacca tgttggccaggctggtcttg aactcctgcc ctcaagtgat ccacctgcct 149280 tggcctccca aagtactgggattacaggca tgagccactg ggcctggctg gcatttctgc 149340 atttttaata ccttgggaacaaccaccacc cctcaaaatg acagtggctc atctttgtca 149400 attatacctt aataaagctggaggaaaatt aataaaatgg actacaaaag aagaagaaga 149460 agaagaagaa gaaaagaaaagaaagtagcc caagttctct gtacctccca gatgccccag 149520 tgaagggcct cagctgtgatgagaaaaccc agcagcccaa ttcctggcaa ctctcctggg 149580 atctggaatg atggtgtgcctgggcacagc cttcctatct ggcccgagag ccagctggca 149640 ccattggagt gaggagagggctctgagctt tcccagtagg ctggcaacct cccaggctcc 149700 ctcccccagc ccctataaaaccatctggaa aatgaaaata ataccttctg cttcctacca 149760 agctccaggg accctgtgaggaacagggag atactatccc cataccccac acattcctgg 149820 aggaggttgc cctggaataagctattgccc cgcctctttc accccaagtg gcagaaacga 149880 gggccactca gtgctctgtccagctgctct tgcttcccct caggagccac ttctgcttcc 149940 atcccacctg attggagtgggcttcccaca ggcccccgct ccgcagctcc tcagtgctct 150000 cctcctcctc cctgctcccccgagcccagc ccagtctgag gtccatcccc ctgtgagggg 150060 agctgagcag tgctgcaaactcagtgctcc tgagtaccct ggggatttgc tgaactgcac 150120 attctcattc tgtaaaccggagctgagcct ctgcacgtct aacctgctcc ctggcaatgc 150180 tgctgttact gagcagagtggaaaagtgct agacaataca ttggggggaa atttcagtct 150240 caaatgcatt ggagcagaaaccagaactcc ctgtcaagct gctctgtggg tttttgaagc 150300 gaagtgtgtt gctcttcactggctgctctg agctgctcag agcgggggac agaagctggg 150360 tctacccgag catctgtccagccacatccc ctcactcttc agggtggcac tgggcacaca 150420 gccccagagt caaggtggcatttagctggg ccttcagtcc ggcaccagca agtgctcttt 150480 aacttggggt ccttggagtccataggtggg cacagctcaa tttggggatg tacctgagaa 150540 aaatgcttcc cagatgggcttctctgcccc atccctctct ccaccgggcc tggccagacc 150600 ccagggtgaa ttgttctaattccttgcact ttttcctggc agggtctcca ggctcatgaa 150660 atctgcctct tgccaaaggcagaggatcca ctggcagcag ggagcactgg ctgcagtcag 150720 gcaggtgcaa tcattttttaatgacatctt ttccccctta tcaaaattgt gcaattcact 150780 ttagaatatt tagaaaatattggaaaatgt aaagaaggaa gtaatcctgc aatcctacaa 150840 ctcagaaatg atgtttcagcactttggaaa atatccttcc aatctttcac atgcacaggt 150900 gtggtctgta tacggcactggagatcccca gcaccaaaaa gttaacactg taggaaaact 150960 atttatccat gtcagttattctttgaaagt atgatgtagg ggaaaaaaaa ggggggatca 151020 gactgttact gtgttgtaaccgagcgagtt atacagaaac gccacactct gagacgaatt 151080 gaggagtcct ttattagccagcgaccgaga gacggctagt gctcaaaatt ctctcggccc 151140 tgaagaaggg gctagattttgttttatact ttggtttaga gaggggaggg ggagcctagc 151200 tgtagcagtc ttacagaagtaaaacaggca aaagaagtta aaaagacaaa tggttacagg 151260 aaagcaaaca gttccaggtgcaggggcttt aaatccatca caaggtgata gacgctgggc 151320 tttgggtgct atcaaccagacaaaaacaca ggggcttagg gtaatatcaa ctggacaaat 151380 tcctgggaac tgcggacatagcttgccaca gcatgttatc agttaattgc attctttgat 151440 gtgctgggag tcagcttgcacaagttaatt ccttgaggaa gggggtgggt aaggagccct 151500 tgatgtcttg caaatgaaggagccaaatgg agtccgttca ggtttctcag ctaagagaga 151560 gtcaatcagg ttaatacaagttagggtatc acaagtgtgt ctatttagaa aaggaagact 151620 taagaaactc cattttgatctgtaccctga acaatggttt tgccttgaga tgctgttaat 151680 ctgtaacttt tctccagccttgtgctcaca gaaacatgtg ttgtatggaa ttaaggttta 151740 agggatctag ggctgtgcaggatgtgcctt gttaacaata tgtttacagg cagtatgctt 151800 ggtaaaagtc atcgccattctccattctcg attaaccagg ggcacgatgc actgcggaaa 151860 gccgcaggga cctctgcccaggaaaaccgg gtattgtcca aagttcctcc ccactgagac 151920 agcctgagat atgtcctcatgggatgggaa agacctgacc gtcccccagc ccgacacccg 151980 tgaagggtct gtgctgaggagaattagtaa aagaggaagg cctctgtctc ataccgaacg 152040 gaatgtctcg gtataaaacccgattgtaga aaaccacact ggcaggaggc gagatatgct 152100 ggcggcaatt attctttattctgttattct ttactacact gagatgtttg ggtggagaga 152160 agcataaatc tggcctacgtgcacatccag gcatagtacc ttcccttgaa cttatttgtg 152220 acacacattc ctttgctcacgttttcttgc tgaccttctc cccactatca ccctgttctc 152280 ctgccgcttc ctcttgctgagatagtgaaa atagtaatca ataaatactg agggaactca 152340 gagaccggtg ctggtgcaggtcctccgtat gctgagcgcc agtctgctgg gcccactgtt 152400 ctttctctat actttgtctctgtgtcttac ttcttttctc agtctctcgt cccacctgac 152460 gagaaatacc cacaggtgtggaggggcaaa ccaccccttc atgtgatgtt taggccgggt 152520 gcaatagccc gcgcctgtaatcccagcact ttgggaggcc gaggtaggag gttcacttga 152580 acccaggagt tcaagaccagcctgggcaac ataatgagac ctcatttcta aaaaataaat 152640 aaataaataa aaattgttttaattagctgg gcatggtggt gcacacctgt agtcacaggt 152700 gctcgagagg ctgaggtgggagaatcagtt cagcccagga gttcaaggct gcagtgagct 152760 gtgatcgcac cactgcactccagcctggac aataaagtga gaccgtgtct caaaaatagt 152820 atgatttttt taatagctgcataatagtct cccctatgga tatttttatt gtttacttaa 152880 tgcttgccct atttggggccattgaagttg tttaattctt tttccaatat aaataatttt 152940 attctggcca tgtttttattgaaatctcta accctataat ctcttttttt ccttagaata 153000 ggtttccagg attgggatattggaacaaaa ggttaaatat tcttaaagtt cctgatagat 153060 gttgttgaat tgtttccagaaagcttcaac aaattcatcc cctctagcca tagtgcctgt 153120 ttcaagaaat gatcagcattgtccattatc ttcttttatt ttttcccaat ttggtaggta 153180 aaactagtat catttctagtctgcattctg cttgactctc gtgtggctga acattttttt 153240 ctgagtggca tagccattcatgtttcttct gtgacgggtc tggacacatc ctcagtgctc 153300 tcattttcag gagagcaaggatggggaacg aggggatatg cattgtatta gtttgttttc 153360 atgctgctga taaagacataccagagactg ggaagaaaaa gaggtttaat tggacttaca 153420 gttccacatg gctggggaggcctcagaatc atggcaggag gtgaaaggca cttcttacat 153480 ggcagcagca agagaaaaagagggagaagc aaaagcagaa aaccctgata aacccatcag 153540 ctctcatgag acttattcactatcacaaga atagcacgag aaagaccggc ccccatgatt 153600 caattacctc accctggccccctcccacaa catgtgggaa ttctgggaga tacaattcaa 153660 gttgagattt gggtggggacacagccaaac tatatcatgc atcaccacct gatggcaccc 153720 acccataacc tctgccatgctgtgtgcccc cccacccatg cctgtgggcg aggctcccca 153780 ggcctggtgc ctttgctgccagactctggg tacttccatt cacactccca tctctgggtg 153840 tcctcaagat aacccgatgttcccaggatc cttccctcaa ttaacaatga cctgccctag 153900 ccccatttct tcagcaccgtctccatgcta agttacaagg agtgggccca aacaaacaac 153960 tgaaagctaa acgtcttcacctaaccaaaa tgttggccgt ctgtattagt ctgttttcat 154020 actgctataa agactacctgagactgggta atttataaag aaaggaggtt taattgactc 154080 acagttctgc atggctggggaggcctcagg aaacttacaa tcatggcaga aggtgaaggg 154140 gaagcaaggc acatcttacaaggcagcaga agagaaagag catgtagaag aaactgccac 154200 tcttaaatca tcagatcacggctgggtgca gtggctcaca cctataatcc cagcactttg 154260 ggaggttgag gcaggcagatcacttgaggt caggagttcg agaccagcct ggccaacatg 154320 gtaaaacccc atcaccactaaaaatacaaa aattagctca ccatggtggc acatgcctgt 154380 aaccccagct acttgggaggctgaggcaag agaatcgctt gacatctggg aggtggaggt 154440 tgcagtgagc caagatcatgccactgtact ccagcctggg caacagagtg ggactccatc 154500 tcaaaaaaaa aaaaaaaaaaggtcagatct tgtgagaact ccctcaccat cacaagaaca 154560 gcattgggga aaccgccttcatgatccaat cactcccacc aggtccctcc cacaacatgg 154620 ggggattaca atttgagatgagatttgggt ggggacacag ggccaaacca tatcaccacc 154680 cacaaaagct ttttgacaaataactctgac taggaattgt aagaaatgtt tttaaatgag 154740 taaggtggaa gcaaacattattcccatttt atagacaaga aatgtgtgtc tctggatctg 154800 tgacactcca taaaccagggaaaaggcaga gacaatgact gattttctgc cgcccaatcc 154860 agttgcccaa ccctcctggctcaatgccag gacttccctg tctttgatta gggaaagtgg 154920 agccacccac tccctacatgcaactgttac acacccagtg tcccagaaat gacattcaac 154980 ttcactatag cgactgcttttactgacatc atttttagca gacgttttac tataaaaatg 155040 ggtggggaga aataaagcagataaactgct gccaaggacc atcactgcta gggtgaaact 155100 gggggcacac ggtatagctcaaatgcaccc caggtccagc ccaagccttg ctcaaaaccc 155160 acccaggaac cagacctatcatccaaggtt gtctctccaa tgaccttagc aggttcagaa 155220 taaccctcta atattcctcatttactgtag ttgcttatta tgaacccacc aactattaat 155280 tcaatctttt ttgaattctcagttttcagt atatttctct tcaggctgtg gtacatatca 155340 caacatatag taattatttgctattttctg cttccctgtc tagactttga gtaagttgag 155400 ggtagaattt gttctatttcataatcttag aatttgacaa atagcaggta ttcttaacca 155460 taattttgtt gaaggaaggaatcaatgaat aaaaatttcc tgagtttact gtttactgca 155520 aaaagactgt atgctgttttacgttttctt cttttaaggt ctaacatcaa tatgttgatg 155580 tttggtaaat ataatttcaatcccagttgc atctctgagt ccaggcaaga gtactacaaa 155640 aagtataaat agaggtcaagtgcagttgcc ctctgcaagc aagaccactt agaactcttc 155700 aatggctctt tgacaaaccttcagtgggat ttctgtcaaa gctgacaatg gataaaatta 155760 tagaaaggag ggaaagccttgataaggagt ccttaatgag ttcgtcacta agttaattca 155820 acattgaaag ggatagaagatgttaattcc acacacacgt gaaagaaatc aacaatcagg 155880 cctcatctga gagaaccatgatggggaagg ctggggctga tggatgaggg gtgtgaggca 155940 gggcagaaag cctccacgttgctgcagcaa tgcaaaaata ggcaccttgc cgtaggcctc 156000 tctggcactc catcttgtctctcttccctg gagaaaaggc aagctgtatt caatcatctt 156060 ttcaatgcta aaaccaggctttcctgtcgg agttggggcc agagatgttt ccatcccctc 156120 ttgcaaaaag ggtattcttaagaggcctta agtcttcagc attgatagtt tctgggaaca 156180 aaatagaaat caaaatctcaccttgtgttc ccttcctctt cctggacaga gaatgggaca 156240 ggcccaggcc atccagatcactatgaacct tatcagaaac ttcagtccat tttctccgtt 156300 cccttccctg ggacttgcatgctataatct ccatacccct ccttccccac tctccaggct 156360 tagtgggtga ggcagagagtgagagccagg cttacctgcc ttttctcctc agtgggaaga 156420 agctcagtaa ctgagctcagacgctagtgg catacctacc aagaggatga cataaatgcc 156480 tcctgtggcc ctgagataggcagagctctg ctggcagccc aaccatatgt tcactcgttc 156540 attaacaaac atccacagaggcaggctgct ctggcctgga gggggatgtc ctacactaga 156600 cgactcaggt tcatagagccaggcctcatt ccacccccac tggctcaagt gactttctgg 156660 tgaccagaaa ctggagagctcttttttttt tttttagacg gggtcttgct ctctcgccca 156720 ggctggagta tactgacacaatctcggctc actgcaacct ctgactccca ggttcaactg 156780 attctcctgc ctcagcctcccaagtagctg ggattacagg cgagtgccac caggcccatc 156840 taattttttg tgttttttagtagagacggg gtttcgccat gttggccagg ctggtctcaa 156900 actcccgatc tcaggcgatccgcccgcctc agcctcccaa agtgctggga ttacaggtgt 156960 gagccaccat gcctggcccagagctcttaa ctacaatgac tatatgcaga agaacaaact 157020 aacacggaag atggctaggaactgcgtaag ccaagactct gcagtaagac acacttgagt 157080 tctggtcctc actctgccgtttactcacag tccaactgca gatgggacct cacctctctg 157140 agcctcagtt tcctcatttgtaaaataggc aaatattggc acctacctca tagaattagt 157200 gcgaggctta actgaggtgatctaggcaaa gcacttagct tggtacctag cacatattaa 157260 gtgctcaata ataggtgatggtgatgatgt aagttggaca caagagaaaa ggacacatac 157320 taactgataa tcacacagagccactgactg gatttggtat ctttctctga cattcagctt 157380 ttcttaccat ctgtcagcatgccacactcc ttcagccttg aattatatgc tgccttgtgg 157440 ttttgctctt ataaaacaaatagaatgtac tagtcttcct gtggctactg aaaaaaaaaa 157500 aaaaaaagac cacaaaccagtgatttacaa cacagaaagt tactctctca cagttctgaa 157560 gaccagaagt ctgaaagtactatgttgtca gggctgtgct ccctccaaag gatctagggg 157620 gtgatctatt acttacttcttccagctttg ggtggctgtc agcatgcctt ggcttgtagc 157680 tgcaccactc caatttctacctagtcttct tatcatctca tctgtgtctg ttttctcctc 157740 agtgtgtctc ttataaaaacacttgtcatg ccagacatgg tggctcatgc ctgtaatccc 157800 agcatgttgg gaggccgaggtgggcagatc acttgaggtc aggggtttga gaccagcctg 157860 gtcaacatgg tgaaaccccatctctactaa aaatacaaaa attagccagg cgtggtggcg 157920 ggctcctgta attgcagctactcaggaggt ggaggcaaga gaattgcttg aaactgggag 157980 gcggaggttg caatgaactgagatcacacc attgcactcc agcctgggtg acagagcaag 158040 actccacctc aaaaaaaaaataataataat aacttggcta atccagggtg atctcctcaa 158100 atcaagatcc ctaatttcattacgtctgca aagttaacat ttacagttct ggaaattagg 158160 atgtagacgt ctttttgggaaccatctatc ttagactgtt caggcaacta taacaaaata 158220 ccagatgctg ggtagcttacaaacaacaaa catttatttc tcacagttct ggaggcttgg 158280 aagtccaaga tcaaggcactggaagctgta gtgtctggtg aggtctttta actatgtcct 158340 cattgcggaa ggggccagggatctctcttc cttttataag gcttctagtc tctttttttt 158400 ctttctttct tttgagacagggtctcactc tgtcccccag gatggagtac agtggcacaa 158460 ttttggctag ctgaaacctctgcctcccag gctcaagtga tcctcccacc tcagcctcct 158520 gagtagctgg gactacaggcgcatgccacc atgtccagct aatttttttt tttttttttt 158580 tttgtagaga tggggttttgccatgttgac aaggctgcca ggctggtctc gcactcctgg 158640 cctcaagcaa tccacccacctcggcctccc aaagtgttgg cattataggt atgagccact 158700 gcacccagct tcattcatgaaggttctatc ttcatgaact aatcatctcc caaataccat 158760 caccctgggg gctgggatttcagcatataa attttgggga gatataaata ttcagtccat 158820 tacaccaaca tttggtccactacttggagt acctaagaac atggacttta gaagtagact 158880 gacctggatt ccaataccagccccaccatc actcactagt tggatgacct tgattcagtt 158940 atttaatatc tttgaacctcagcttctgga aaagggccaa taatgccaat aatgctatgt 159000 cccggggttg tttgaaagaattaaataagc taatatgtta aaagtactgg ataattggta 159060 gctctcatta ctagtggtaggtttacccca ctagtctaca agctcttaag agctggagtt 159120 atctctgctt ccttcacaagtctagcacaa gtctgcaaac agtctatcct tagtgaaggt 159180 ggggtgacaa actggctgaaccagtggcct agacagccaa tatcaccaat tatgtaagat 159240 tccacaggga tctaaaggagcgaattagag gcacccaata gtgaccccaa tctaagcagt 159300 gcagggatca gctgggtgggtttgtcagca gagaacaggg ccaccagggc aggcacatag 159360 aactaactgc actcactgcactgttgttaa ctattctttg aatccctcca catgacgcca 159420 ttcctacaga tggaaggtcagctacactgg agaagttcca gaaattcata aatagattat 159480 gatcccaaat cccagagaggaggacttttc cagatggcaa ctgggaattt accttaatga 159540 tttccccatc ccagaagggaaagctgttct ggtatattac aaacattaca gcacatctga 159600 ataacccaga gagcttgtttaaatgcacat ttttgggcct ctaccaggct ggttaaacaa 159660 agcttcaaca gagtggggataggaaccaac cattttaaat aacccccttt ggtaattctg 159720 gttcaaccat agcaatcaaattatagtcca acctaggtag gggcttgcta cctaggagtc 159780 aatatgtaat actggaaagagtaagagttt ggaaactaaa cagaaatagg gtccaatcct 159840 gggtcgtcca cataatagctgtgggacctt gggcaagtta cttcattttt ttggatctca 159900 acttcctctt ctataaagggggggctatac ctgtcagggt agtggtgtta attcaatgat 159960 aattatgtaa agtgcctgctcatagtagat tgaagcagag actgctacta ttcaccaatc 160020 catttcttct tcttgttcatacacaactag actatatttc ccagcaccct ttgcagccag 160080 gcacggccac actagttctcaccaagggga tatggttcta agttatagta cctattcttg 160140 gcaaaggctt aaggaacagatatgcctcct ccaggttttc ttttgtctta catcacttgg 160200 atgcaggtga caggttctagggaactgggg atctacaaca tggaaggagc ctgtatgcct 160260 gagttactag gtaggggaaagctatccatc aactagaatg cctgccttgg actcttctgc 160320 aaacgagaaa taagctcatataccatatta ggttataata tagttgcttt aaagaaatac 160380 ccatggccag gcgtgggggcttacacctcc cagcactttg ggaggctgag gcaggaggat 160440 cacttgaggc caggagtttgagaccagcct ggccaacatg gtgaaacccc atctctactg 160500 aaaatacaaa aaattagccaggtgtggggg caggcgcctg taatcccagc tattcgggtg 160560 gctgaggcac aagaatcgcttgaacccagg aggcagaggt tgcgtgagcc gagtttcagg 160620 ctgcactcca gcctgggggacagagtgaga ctctgtctca aaaaaagaaa agaaatacct 160680 gcattgctat aaagaaatacctgagacttt aatttataaa gaaaggagtt ttggaggctt 160740 tttctttttt ttgagacagaatttcgctct tgttgtccag gctggagtgc aatggcgcga 160800 tcttgactca ctgcaacctccgcctcccag gttccagtga ttctcctgcc tcagcctcct 160860 gagtagctgg gactataggcatgtgccacc acacctggct aattttatat ttttagtaga 160920 gatggggttt ctccatgttggtcaggctgg tcccgaactc ccaacctcag gtgatccgcc 160980 cactttggcc tcccaaagtactgggattac aggcatgagc caacgcaccc agccaagaaa 161040 ggagtttgtt tgtttgtctgtttttccaag aaaggagttt taactggctc atgattctac 161100 aggctgcaca agcaaggtgctggcatctgc tcagtttctg gggtggccta agggagcttt 161160 tacttaaagc cagagcaagcatctcacatg gcagagtaga atcaagagtg agagttggtg 161220 tggtggtgag gaatctcaggatgacctccc tgagattgct gtcccctagt tattcacaaa 161280 aactcactga ctatcacaaggtcagcatca aggcgtgagg catccacccc catgacccaa 161340 acgccaggcc ccacctccaacactgaggat tacatctcga catgagattt ggaggggcca 161400 tctaaactat atcatacacttttgggatct attttacaac taacctatag tcagtgtgtt 161460 aatccatttt gcattgctattaagaaatac ctgagactgg gtaatttatt ttaaaaagag 161520 gtttattttg ccttatggttctgcaggctg tacaaccatg gcaccagcat ctgcttctgg 161580 tgaggactca agaaactaccaatcatggca gaagcaggtg tgtcacatgg cgacagagag 161640 agaaagagag ggggaggagatgccaggctc ttttaaacaa ctagctctca tgtgaactta 161700 atagagtgag aacccactcattacctcggg gagggcacaa aaccattcat gagggatctg 161760 cccccacgac ccagacacctcccaccaggt cccatcttca accctgggaa tcacatttca 161820 acctgagatt tggaggggacacacatccaa accatatcag tcaggaaatg ttcaccccct 161880 ttctccacct ccaaataacaattacacagt acccagcatc tgtgcaaaca ttttattcta 161940 ttcacaattc tcatatggtgtaaattgcaa tctctctgta gtaggacaaa ttctagggat 162000 gacctctgta ttagtcaggcttctctagag ggacagaact aatagtatat atatatatat 162060 aatatgagtt tattaaatattagctcacac gatcacaacg tcccacaata ggccatctgc 162120 aggccgatct gatgtccaatgtttgagggc aggaagcatc cagcacggga gaaagatgta 162180 gagtgggagg ctagacccatctttcttttc acatttttct gcctgcttat attctagcag 162240 tgctggcagc tgattagactgtgcccaccc agattaagag tgggtctgcc tttcccagcc 162300 cactgactca aatgttaatcccttttggca ataccctcac agacacaccc aagatcaatc 162360 ctttcaatcc aatcaagttgacactcagta ttaaccatca taacctccct gagaaacaca 162420 aatctaggta ctcctgtgaagggattctgc aaatgtaatt aaagtttcaa gtcagtcaac 162480 cttaaaatac agagatgatctgggtgatcc tgatctaatc acaagagccc tttacacaac 162540 agaaggggaa ttcggagagatttgaagtat gaggaagact caagtgccat tgctgcttga 162600 agatggcagc accacgtgagaaggaatacc tcaaggaact gaaagtggcc cttgcctaac 162660 agtcagcaag gaaagaggaacctcagacct acagctgtaa ggaactggat tcagccaata 162720 attggaatga acttggaagcagatttttct cagtgcctcc aggtaaaagc tctgcaacca 162780 ataccttgat ttccagccctgtgctattcc tgagcataga aatcaggcaa gcctgcctgg 162840 acttctgact acagaactgtccactaataa atttatgttg ttaaatttgt ggtgatttgt 162900 cacagcagca ataaaaaaactaatacattc tcttaatctg ttttgtcatc aatttttctt 162960 tgagacggag tctcactctctttcccagcc tggagtgcag tggtgtgatc atagcccatt 163020 gtaacctcaa aatcgtgggctcaagcagtt ctcccacctc aatctctcga gtagctagga 163080 ctacaggcac atgccaccatacttggctga tttttttaaa aaaattattt tttatgtaga 163140 gatggggtct cactatgttgcccaggctgg tctcaaactc ctggcttgaa gagatccttc 163200 tatctcagct gggaggatcccaggatccca aagcgctggg attacaggcg tgagcctcca 163260 tgcctagctt cctcacttattttttattaa taaaaagtga catggggaga tggtggaagt 163320 tagagtgatg atatctgagtatgctcttag ctttacattt gctcagttcc ttgtgctgag 163380 ttgtctgtta gggagcttcctgcccttata gtgagaatgt gacatagtct gggcatcagc 163440 tgcagctctt tgaacagagatgatccagct ccccatctca ccagccccca aatccaaaac 163500 ttaaaagcgg agctgctgtcaaggaaaaca aagttaagca aaatgacttc aaagcccaat 163560 gtgctcacct tcactctcccagtgggtcct tgcacctgag aacgtgcagg gtccatcagt 163620 attgacctcc cctttccaccagccctggaa gttatggaag gggaaggtag cttattctga 163680 tatcgtgtcc tgtaataatgcttcctggcc tggcacatat atccttgttt ctgtgcctct 163740 tgttctaaaa catcctccctttccgaaagc tgaaactgtt cctgcacaca ttatgcactt 163800 aaccaggtaa atttcagaagattttaaaaa gtttcccagt gaaggaagga gagagaggta 163860 ttgtcctgag atactgaatatgcggatggc cagactagat atcaaaatag aaccctgacc 163920 cacaatctgt ggcaactagcctgggaagcc aacatattat ctgcagtaac caatccagga 163980 agcaaagcaa taacccccttagcaatggac cccaaatggc caggactcga ttaataaact 164040 gacagctccc ataatttttgcccctacctc caactttgga ctaaccagag aaagccaaat 164100 gggcaccccc aaccaatcacatagcatgcc gcacttctag ctggcccact tgcagcttcc 164160 ccgtgccaac agtctcagggcacgcccaaa gtgttccctt ttttctacca caaggctttt 164220 ccacacctct gcctgcctttaagtgtctgc caaacacaag tgatggtggc tgactacctt 164280 gcaacctctc tatagtaggctttgtttgtt cttatttggg tggtcttcat ttatttccac 164340 agtccttgtt tttcagggagatttagggct taaatacaag gaaatttcat gtaaggatgc 164400 aggagtgttt catggaacctcaagctcagg aaagctgcac agccatggga agaccctaga 164460 gccggagtct ggaaaagcaatccgagcttg gatcttagaa atcttgtctt cttgcaaacc 164520 gggttctctg gtccaaatggccaaaaacat gaccatggac agggcctgtg cttatctcgt 164580 cacctgcaga gaggcaactctctgtacgtc ctgattccaa gttcctggag aaggacacag 164640 actgaaccac cttgggtcaggcaccatcct tgaccaattt actaacagtg cccaactccc 164700 acccagggtg gggagtagttgcaggtagtc ttgtttatga acatggcaat cagaagccca 164760 tccctgtcac tgtgtggaggggaggggaga gaagagggat ggttccaacc aaaaaaggta 164820 gggggatgga catcccaataggggtccact ccattcctga tggcacagtg agacagttgt 164880 catgtgtcac gtgctagactcttgactctg tgcctcccag tacctgctac tgaccaacat 164940 ggtctagtga gcgatttttcttgtgaaggc atccatccat tcattcattc atatagcaaa 165000 caattgtaca tcacaaccttatgctaggtg ctaggtatac aatgataaaa caaacacaaa 165060 aaccaaaacc acaatctctaccctcaatgt gaggagatac caccacataa acgaacaatt 165120 acaatactgt gaaacatgttatgctcaatc tatggcctaa atcagatgag aggctgggca 165180 gaggatcagg ctgccaagaaatgcttccta gagggcatgc tgcccgaaat gaaccttgaa 165240 aatcagggag cttgatagaaatagggaaag agagagtgtt tcaggcagaa gcaaggttca 165300 ctgtcaagga aaggcatttcaggaaaccaa aagtagtatc tgtttgacat gagctatatt 165360 ggagtgagag tactgggaggtaggctagaa aggggaacag agactagata tgaaggaact 165420 tgaaagccgt aagtatatgaagaaggcaat gggctttcta cacaggaatg accttgaaga 165480 cttgatttgc attattgaaagctcaccatg gctggaatgg attggggacg tccgatgatg 165540 ggaagactca gctgggcagttgctgcatta actgtggagt tataatggtg acctacgaga 165600 gcagctttgg atacggagaagagcaaactg gtttaagaaa cgtcatcacc tgggtcaaga 165660 aaaggacatg cagtgggttgggtagggagg aaaatagatg taaataaagt tattagttga 165720 aaaagaaaag caagatcgagagagaaatta attctattgc catctattca tgagaatcac 165780 aaggctgaat caaaagccttcctgcaaacc tagactcacg ctggttcagt tcagagactc 165840 tatcagaaag atgaggagctcatgagtcat ccatcaatgt gatcttgtga tttttcttgt 165900 gaccacaaga caaaggaagagaagagtact ttggagtcat tatgatgaaa attcagttga 165960 atacactgga aagtcaaaacgagaggagaa ggaggaaccg aaaatctgat tcagtcttga 166020 ccaccaagta gaattgggacagaaacaatt aagtaggcac agatccaatt tggattataa 166080 aaagctggtt catcaggaaggccactataa aaccagaagc aaacgaagag tcaattaagt 166140 acaaaagttt ttactatgactatgcagtga ggtccaaact gtactaaatt tgaagttccc 166200 tgccaaagag aaatgtaagcttccctggtg gtataagtca gaatttcatg acatcagcca 166260 gataaccaca aaagaatatatcttttagtg ctagatgctt ctaagccttt ctccacccaa 166320 ctaaccaaca ttcacttactgatttactta tttctttaac aaacacactc catttcatag 166380 ccacattctt tcaaagctgacaggttgaga tatatctaaa acacaaaatc tccaacatac 166440 attttcttct tcaatgttgtgagatgtatt tttctaaaac actgttctgc acatactacc 166500 attccaatat taaaaacaagttagttatcc actgcctttt ttgtacaaat gaaattcata 166560 aattttaatt tggttacaaaaaaatttttc atcaaaattt ttgcttattt gtttatataa 166620 aaatggagac agggtctcactatgttgccc aggctggcct caaactccta agctcaagtg 166680 atcttcccac cttgacctcccaaagtgata ggattacagg catgcgatac caagcctggt 166740 ataatttaaa tttttaattaaaaattgtta atttgtctac aaaataaata tatactcatc 166800 atcaaaaact ttcaaagaagaagattaaaa tcaccataat tccaacaccc agacacaatc 166860 acaatcactt ctgtaagttaggatatactg acagcctatg tgacagggaa aaaactaagt 166920 tgtaagaatt ttttaaaaacctcctattcc ccattacagt agattttatt actcaaaata 166980 tttgctgccc caccctggggaagataatac ttccccactt cattgcatca gccttagaga 167040 tgtggtttga ggtgtcccttcttgtgggag gattacatat tctcatctcc ttgacctcaa 167100 gagtggccat gagacttgctttgaccaatg caatgataac agaagtgatg tgtgccactt 167160 cgaagcagaa gctctgattcatcatatggt tctactatgt tcgttctctc ttccatgaaa 167220 ccagcaatat cccaaatacagactgctggt cccaggatgg aaaacttaga gcaaagctac 167280 cggtagccca caatgtgagcaagaactgaa ctttagtcat tgtaagccac tgagggtttt 167340 ggagttgctt gttaccacagcataacttag ccgacactga ctgataccac ctacctccaa 167400 aatgtcatct gagttttctttatgtctgaa gagagccttt atgcattgaa ataaatactc 167460 cctttaattt caacttatattttatatatt taaattatat gttttaaaac ttaattttga 167520 agactttaaa cttgtgttaatttataaatc agttttgtta atattttatt atctactttt 167580 atttggtttt gatgaatgcccaactggctt gtaaatttga taaggtactc ccaaatattt 167640 atcaaacttt cagaattaagttttagacat ttcaaactac aatctaattt taattgattt 167700 ctcaaggaaa aaaacaacaaagcagactca catttctata ggcaaaatct taataaacac 167760 tgaaaacata ctgaacttaaaaagtttaac cttacaaatt taataaatta taaatttaaa 167820 gatttaacaa tttaaacatttgaacattat ttcaaactta gttatcaaca tcttataaat 167880 ttttactaat attttgagtctaatgaatca gtctctttac ttaccttaaa aagcagaaaa 167940 atatacacaa aatactataatggttacaaa acttatttct agataaaatt tatgactaga 168000 cataacttag cttgtcttccaagggtcttt gaaagtatgt ttgttttctg gcaataagaa 168060 gatatttcca gactcatcttgatatttcct gccgtaaaac atgcaatgaa tttttctcca 168120 gggagcccag tgccctcagggtcttctggc tggaatgcag attagatgga ttggcaaagc 168180 agctgctggg gccacaaggggaaaccagaa atccacagta atctttgacc tgacgtcctt 168240 gagctgctga accaacatcccaggttttta agtccctgtg gtccatttgt caaatgcaat 168300 tccttactga tataatggttatacaaataa agaagcccag agaaagcact gcatatggaa 168360 aggaaagatt gacctaaaggggaagatctt gtggggaggg gaggcagtga agaaaagagg 168420 aggcaggaaa gggaactggggagagagggg agatctggca gtttctgtga aagcaaatta 168480 gtaaggattt taagaaaagtttgcctttat tccattgcca taatggggat tagaacagta 168540 acatccatag gatcattgtgagaattatgc caagcacaca ttaagtgttc aataaacatt 168600 ggctatcatt attattttattatgctgggc ctccagaatg gattagagtt gcagactgga 168660 acattataag gaaatccaggaagtccattc attcttactc tttccctccc tctcctctct 168720 tccacaattg ctcctctgcacagctgtttt aatcctatat ctttctgcag attggctttc 168780 tttctatgtc tgtgatgcttaaaacagtca ctaacaacat ctttcaagtt tccaaactcc 168840 ttggttcaag acagcagccatttcgaagaa atttccagaa agacactgat tgtccagctt 168900 agatggaaag tccactcttggatcaattaa ctgaagctat gacaggagag ttgtatggaa 168960 aacacatggc tgctcctactagaaaacttc ccaaaatatg aaggagggaa aagcagggag 169020 gaggtgccgc tgtgaagacgtaatggtagg taaacatttg ggtacatttc ctttgggtcc 169080 atgtgcacac gagtacgtttatgcatgtgt gcataacttg gatagcactg tatataggtt 169140 ttgtttgttt gtttgattgttttagagaca gggtctccct ctgtcactga ggctggagtg 169200 cagtgatgca atcatagctcactgcagcct ggaactcatc tgctgcactc aagggatctt 169260 cccacctcag ccttaagagcagctgggatt acaggtgtgc atcaccatgc ctggataagt 169320 tttaaaacaa tttttttgtagagacagggt ctcattatgt tgctcaggct ggtcttgaac 169380 tcctggcctc acgtgatcctcccaccccag cctcccaagt agctggaatt ataggcatga 169440 gccactgtgc ctggcaacagctgtctaaat tattttttac aaggcattca gaacacattc 169500 aataagatat ttttctacagcactgtattt attagctagg ttaacatttc catatataga 169560 tgttctaagt gaatttaaattatttctatt attttgcttt tatgaactct atttgagtga 169620 tattcttgta actgaatagttgaaactatt attaactcct tagggaaatt ccttaaattg 169680 gaattgcgag gtcagaaggtgtgtcatttt ttaacagttt tgatacattt tgctaaatgt 169740 gccaggcaat ttaagccacactcccaccac aacatacaaa atgcagtctt cctcaatatc 169800 gtcttcagtg cttagttgaaaattaatcac catttgataa gtgaaacaaa atttccttaa 169860 ttactagtaa gattgaaggatagtttcatg tttttttgac caactgtttt tctattatat 169920 tcttattggt ttataagagcattttagccg gacttggtgg ctcatgccta taatcccagc 169980 actttgggag gccaaggtgggtggatcact tgaggtcagg agttcaagac cagcctggcc 170040 aacatgatga aaccccaactctactaaaaa tacaaaaact agccgggcct ggtggcaggc 170100 acctgtaatc ccagctactcgggaggctga ggcaggagaa tcacttgaac ccaggaggca 170160 gaggttgcag tgagccaagatcgcgccact gcactccaac ctgggcaaca aagcgagatt 170220 ccatctcaaa aaaaaaaaagaaggcatttt aattactaaa tatattgctg gttgtggtgg 170280 cttgtgtctg tagtcccagctacttggttg aggccaagag tttgagcctg ggcaatacaa 170340 cagggcccta tctgtaattacatcttcccg gtttgtcgta accttgatat aatttgtttt 170400 aagttcagat actcaaatctcttctttcca gcacctatct ttatctttcc atatgcctag 170460 aaggatatca tccactatctacctaagtat caccctttcc tatgtcccat tccctaaata 170520 gagtttagac atttcaattggcatttaaaa gtccttcaca aggcactacg atgtcttgtt 170580 tttcactact gccatccacaaatctttctt ttatccatcc atttgttcat ttattcattt 170640 gtcacctatt tactaagcacctattatgta ccaggcatca ttctagacac tggggataca 170700 ggagtgaaca catccctacccctaagggag cttgaatttt agtgccactg aagacaaact 170760 gaaaaggtaa gaaaatgtatatagttggct ggatggtgat aagtactata gaaaacaata 170820 aagcagtaaa gtagggtaggaaattcaggg agagagtgaa atcttacata gaatagtcag 170880 ggagaacttc actgaaaatgtaatacatat ttgaggaaga gtgtgaggat ggtgactgat 170940 atggtttggc tgtgtccctacccaaatctc atcttgaact gtagctccca taattcccat 171000 gtgtcatggg ggggacctggtgagaggtaa ctgaatcatg ggactgggtc tttcccatgc 171060 tgttctcata gtggtaaataagtctcacga gatctgatgg ttttataaat ggtagaccct 171120 acacactctc ttgcctgccaccatgtaaga catgactttg ctcctcattc gccttcagct 171180 atgattctga ggcctccccagccatgtgga gctgtgaatc aattaaacct ttttcattta 171240 taaattaccc agtcttgggtatgtctttat tagcagcgtg agaacagact gatacagtga 171300 cagaatgggc caagtgctatccaagggaga agcattccag tatgggaaag agtgtgtgca 171360 gagagcctgc gggggagagggggaaggagg agcatacctc ctggatttga acaccagcaa 171420 ggaagccagt aatgctggaacagagtgggc aaagagggta gttgaggcag ggatagggaa 171480 tgggtcatgc agggttttgtagaccactgt agagtctttg gcttttactt caaaggaaac 171540 gggaagccat tttgagggtgtggtaaattg tgtttccaaa gatggcagca gaaatatctc 171600 acacctcaca tagtcttttgcaatgtgatc ttgtcactgt cctaagaaga gatggggtct 171660 ctttcctttt tccttgaatttgggctgagc ctaaaccaat cctgacgaac agaatctagt 171720 tctgtaacta gattctgtaatactgtaaat tccagactgg gccttaggaa ctgttttaga 171780 agccttcctt tcaccgcttgaaatgctcct tcttggaacc cagaaggcat atggagaaag 171840 atacgtagaa gagaaccaaggcccagccat ttcggcctag ttgagcattt ctgccagccc 171900 ctagctttca agccagccagttgaagccgc ggacaccaga taacagagat gagccatgtt 171960 caacatgctc tgcacaaatttttcaccccc aaaccatgag caaataatag caattgttgt 172020 tttaaaccat taagttttagtgtgatttgt tactgatgac aaaagagaca aatagagatg 172080 aactaaatag cacgggccacaggggagtag gcacgagacc ccacagttac tttcctgaaa 172140 ttcctagacc ccctcctgcttctgtaagga actccaggtc tgggttaatc acaaactgat 172200 gactattcca agaagcactgtactccttag ctaatcatct gttagcttat ttctgttaac 172260 taatggtatt tctatatgaacatgctgaga ccaccttgtt gtatctcctc atgttgttcc 172320 ttaaaatctg cttgtaaccaccccaacctg gagcacctcc tggacttggt atataagtgc 172380 ttcccaggtt gcaatcctcaaatttggccc aaataaactc tttacctata tgcatttgcc 172440 ttagttcttt ttttttttatttccaccatg cctggctaat ttttaaattt tttgtagagt 172500 caagatctca ttatgttgctcaggctggtc tcaaactctg gggatcaagt gatcctccta 172560 cctcggcctc ccaatgtgctgggattacag gtgtgagcca ccgtgcctgg cccttagttc 172620 tttctttcag attgacattacacagcaata ggggactgga ataaaaattg gcaccagaac 172680 atgaaatact actggaatgaaaacctaaag ccaattgcta catgtttggg accaagtaac 172740 acatagaaga ctgaagagcagtgagaaggc tgttggtgtc ttcacatggt ttgatatttg 172800 tccctcccaa atctcatgttgaaatgtaat cctcagcgtt ggaggtgggg cctggtggga 172860 ggtgtcatgg ttgcagatccctcatggctt ggtgctgtct tcgcaatagt aagttctcat 172920 gagatctggt tgtgtgtggcacctctccct acctgctcct cttcttgcca tgtgacgtgc 172980 ctgctcgcac ttcaccttccacagtgagta aaagctcact gaggcctctc cagaagccga 173040 gcagatgcca gtaccaatgcttatactgcc tgcagaacta tgagccaaat aaaccccttt 173100 tctctataaa ttacccagtcttgggtattc ctttagagca atgcaagaaa ggcctaacac 173160 tagcctaaaa cagggaggaaaaacttgctc ttgctcttgt gggctagaga aaagggggcc 173220 tgagttacat actggcagctcaattacact gtcacttgta gcaatgtgga agatggtaca 173280 tgtgttcatg aactggctaaggacattttc atgcagagtg ctgaaaatgc caagtggctt 173340 gtaatagttg ataatgtacagaaagagata aactaaagag ggaaatgttc acctttcaag 173400 caaattttag aagaatatagagggcccaag actgtctgag tcagttagca gcgtgttcta 173460 aaatcaaaat aaaacttggaggcaaagaac aaatctaaga tgctgtaacc gcccaacgaa 173520 ttttccttgc ccactgcctaggcagactca atttaagaca ggggaattac aataaagagt 173580 ttaatccaca cacagtcatctgtacgggag actggagttg tattattacg caaatcagtc 173640 tccctaaaga cttgggatggggtttttaag gataatttgg tgggcagggg gtcagaaagt 173700 ggggactact gattggttaggtcagagatg aaatcacagg gagttgaagc tgtcctctgg 173760 tgctgagtca gttcctgggtgggggccaca agaccagatg aaccaattta tcaatctggt 173820 tagggacagc tgatccatcgagtgcagggt ctccaaaata cctgttatct ggaggagcaa 173880 tttggagggg tttagaatcttgccacctcc agctgcatga ctcctaaacc acaatttcta 173940 atcttgtagc taatttgttagtcctgcaaa ggcagtttat agttcccagg ctaaaagggt 174000 gtttgttcag ggaaagggctggtaccatct ttgtttcaaa gttaaactat aggccgggcg 174060 tggtggctca cacctgtaatcccagcacgt tggaaggccg aggcaggcag atcatttgag 174120 atcaggagtt cgagaccagcctggccaaca tggtgaaacc ctgtctctac taaaaataca 174180 aaaattagtc tggcgtggtggcgtgcacct gtagtcccag ctactcagga ggctgacgca 174240 ggagaatcgt ttgaacccaggaggtggaga ttgcagtgag ccaagatggg gccactgcac 174300 tccagcctgg gtgatagagtgagactctgt ctcaaaacaa aacaaaacaa aataaacaac 174360 aacaacaaaa aacaaagttaaactgtaagt taagttcctt ccaaaattag ttcagcctac 174420 acccaggaat gaacaaggacagcttggagg ttaggaacaa atcagtaagt taggtcacat 174480 ctctttcatt gtcataatcctctcagttat aatttatgca aaggtggttt cgatgccaca 174540 agtaaaacgt ggcctctggtcaacatgagg ccctttgtta aaatcccaga aagatgtaag 174600 gaattgcttc tcaaaccctcatggttagtt agacaaacag gcttctgaaa gaacaagtgt 174660 gttctcacac agcaccctgacatgcacccc agagagggag cgtatcttaa aaagattggt 174720 ggagatggtt tttatctaacacagtgaatc ccagtaaaat ccacagaaaa ctcacaatgt 174780 ttttaagaga actgaactaacacaagcacc actagcttgg actaaaatgg acaaagactg 174840 ttcaaaacaa agccactgggctcctaacca tttactggca ggaaataggc tgaggaaact 174900 atttggctgc aaatcagggtttttgttgtt gagatagggt ctcctccgtt gcccaggctg 174960 gagcacaatg gcacaatcacaactcactat aacctctgcc tccctggagt agctaggact 175020 ataggcacat gccaccaagcccgcctaatt tttgtatttt ttgtagagac ggggtttcac 175080 catattgccc aggctggtcttgaactccca gcctcaagtg atcctcccgc gtcagcctcc 175140 caaaatgttg ggattacaggcatgagccac cacgccaggc ccaatcacgc tttttcaaat 175200 agtaaaggaa ggccgacccagaaggtggag ttaggaagta aatcaggtcc agctgtgtgt 175260 ggataaggct ccacagggatggctctgtgg gtgcagggac ccaagaacta aaaagacaag 175320 ttatctgcct cccacattccttgtacaatg tgaggaggaa aaagataact acaatagaga 175380 atccctttca aaaagtaaggatgagtgtat gaaatctgaa taaggtttgc agtgcagttt 175440 aaacagttca tagtgtccagtgcttctgat aggtcaagta aagtaaggac gatggacgtg 175500 gcaacatgga agtcacccatgacttcgaaa agcagttttc aatttaagtg tgtgaaagct 175560 t 175561 4 961 PRTHomo sapiens 4 Met Leu Ala Pro Arg Gly Ala Ala Val Leu Leu Leu His LeuVal Leu 1 5 10 15 Gln Arg Trp Leu Ala Ala Gly Ala Gln Ala Thr Pro GlnVal Phe Asp 20 25 30 Leu Leu Pro Ser Ser Ser Gln Arg Leu Asn Pro Gly AlaLeu Leu Pro 35 40 45 Val Leu Thr Asp Pro Ala Leu Asn Asp Leu Tyr Val IleSer Thr Phe 50 55 60 Lys Leu Gln Thr Lys Ser Ser Ala Thr Ile Phe Gly LeuTyr Ser Ser 65 70 75 80 Thr Asp Asn Ser Lys Tyr Phe Glu Phe Thr Val MetGly Arg Leu Ser 85 90 95 Lys Ala Ile Leu Arg Tyr Leu Lys Asn Asp Gly LysVal His Leu Val 100 105 110 Val Phe Asn Asn Leu Gln Leu Ala Asp Gly ArgArg His Arg Ile Leu 115 120 125 Leu Arg Leu Ser Asn Leu Gln Arg Gly AlaGly Ser Leu Glu Leu Tyr 130 135 140 Leu Asp Cys Ile Gln Val Asp Ser ValHis Asn Leu Pro Arg Ala Phe 145 150 155 160 Ala Gly Pro Ser Gln Lys ProGlu Thr Ile Glu Leu Arg Thr Phe Gln 165 170 175 Arg Lys Pro Gln Asp PheLeu Glu Glu Leu Lys Leu Val Val Arg Gly 180 185 190 Ser Leu Phe Gln ValAla Ser Leu Gln Asp Cys Phe Leu Gln Gln Ser 195 200 205 Glu Pro Leu AlaAla Thr Gly Thr Gly Asp Phe Asn Arg Gln Phe Leu 210 215 220 Gly Gln MetThr Gln Leu Asn Gln Leu Leu Gly Glu Val Lys Asp Leu 225 230 235 240 LeuArg Gln Gln Val Lys Glu Thr Ser Phe Leu Arg Asn Thr Ile Ala 245 250 255Glu Cys Gln Ala Cys Gly Pro Leu Lys Phe Gln Ser Pro Thr Pro Ser 260 265270 Thr Val Val Ala Pro Ala Pro Pro Ala Pro Pro Thr Arg Pro Pro Arg 275280 285 Arg Cys Asp Ser Asn Pro Cys Phe Arg Gly Val Gln Cys Thr Asp Ser290 295 300 Arg Asp Gly Phe Gln Cys Gly Pro Cys Pro Glu Gly Tyr Thr GlyAsn 305 310 315 320 Gly Ile Thr Cys Ile Asp Val Asp Glu Cys Lys Tyr HisPro Cys Tyr 325 330 335 Pro Gly Val His Cys Ile Asn Leu Ser Pro Gly PheArg Cys Asp Ala 340 345 350 Cys Pro Val Gly Phe Thr Gly Pro Met Val GlnGly Val Gly Ile Ser 355 360 365 Phe Ala Lys Ser Asn Lys Gln Val Cys ThrAsp Ile Asp Glu Cys Arg 370 375 380 Asn Gly Ala Cys Val Pro Asn Ser IleCys Val Asn Thr Leu Gly Ser 385 390 395 400 Tyr Arg Cys Gly Pro Cys LysPro Gly Tyr Thr Gly Asp Gln Ile Arg 405 410 415 Gly Cys Lys Val Glu ArgAsn Cys Arg Asn Pro Glu Leu Asn Pro Cys 420 425 430 Ser Val Asn Ala GlnCys Ile Glu Glu Arg Gln Gly Asp Val Thr Cys 435 440 445 Val Cys Gly ValGly Trp Ala Gly Asp Gly Tyr Ile Cys Gly Lys Asp 450 455 460 Val Asp IleAsp Ser Tyr Pro Asp Glu Glu Leu Pro Cys Ser Ala Arg 465 470 475 480 AsnCys Lys Lys Asp Asn Cys Lys Tyr Val Pro Asn Ser Gly Gln Glu 485 490 495Asp Ala Asp Arg Asp Gly Ile Gly Asp Ala Cys Asp Glu Asp Ala Asp 500 505510 Gly Asp Gly Ile Leu Asn Glu Gln Asp Asn Cys Val Leu Ile His Asn 515520 525 Val Asp Gln Arg Asn Ser Asp Lys Asp Ile Phe Gly Asp Ala Cys Asp530 535 540 Asn Cys Leu Ser Val Leu Asn Asn Asp Gln Lys Asp Thr Asp GlyAsp 545 550 555 560 Gly Arg Gly Asp Ala Cys Asp Asp Asp Met Asp Gly AspGly Ile Lys 565 570 575 Asn Ile Leu Asp Asn Cys Pro Lys Phe Pro Asn ArgAsp Gln Arg Asp 580 585 590 Lys Asp Gly Asp Gly Val Gly Asp Ala Cys AspSer Cys Pro Asp Val 595 600 605 Ser Asn Pro Asn Gln Ser Asp Val Asp AsnAsp Leu Val Gly Asp Ser 610 615 620 Cys Asp Thr Asn Gln Asp Ser Asp GlyAsp Gly His Gln Asp Ser Thr 625 630 635 640 Asp Asn Cys Pro Thr Val IleAsn Ser Ala Gln Leu Asp Thr Asp Lys 645 650 655 Asp Gly Ile Gly Asp GluCys Asp Asp Asp Asp Asp Asn Asp Gly Ile 660 665 670 Pro Asp Leu Val ProPro Gly Pro Asp Asn Cys Arg Leu Val Pro Asn 675 680 685 Pro Ala Gln GluAsp Ser Asn Ser Asp Gly Val Gly Asp Ile Cys Glu 690 695 700 Ser Asp PheAsp Gln Asp Gln Val Ile Asp Arg Ile Asp Val Cys Pro 705 710 715 720 GluAsn Ala Glu Val Thr Leu Thr Asp Phe Arg Ala Tyr Gln Thr Val 725 730 735Gly Leu Asp Pro Glu Gly Asp Ala Gln Ile Asp Pro Asn Trp Val Val 740 745750 Leu Asn Gln Gly Met Glu Ile Val Gln Thr Met Asn Ser Asp Pro Gly 755760 765 Leu Ala Val Gly Tyr Thr Ala Phe Asn Gly Val Asp Phe Glu Gly Thr770 775 780 Phe His Val Asn Thr Gln Thr Asp Asp Asp Tyr Ala Gly Phe IlePhe 785 790 795 800 Gly Tyr Gln Asp Ser Ser Ser Phe Tyr Val Val Met TrpLys Gln Thr 805 810 815 Glu Gln Thr Tyr Trp Gln Ala Thr Pro Phe Arg AlaVal Ala Glu Pro 820 825 830 Gly Ile Gln Leu Lys Ala Val Lys Ser Lys ThrGly Pro Gly Glu His 835 840 845 Leu Arg Asn Ser Leu Trp His Thr Gly AspThr Ser Asp Gln Val Arg 850 855 860 Leu Leu Trp Lys Asp Ser Arg Asn ValGly Trp Lys Asp Lys Val Ser 865 870 875 880 Tyr Arg Trp Phe Leu Gln HisArg Pro Gln Val Gly Tyr Ile Arg Val 885 890 895 Arg Phe Tyr Glu Gly SerGlu Leu Val Ala Asp Ser Gly Val Thr Ile 900 905 910 Asp Thr Thr Met ArgGly Gly Arg Leu Gly Val Phe Cys Phe Ser Gln 915 920 925 Glu Asn Ile IleTrp Ser Asn Leu Lys Tyr Arg Cys Asn Asp Thr Ile 930 935 940 Pro Glu AspPhe Gln Glu Phe Gln Thr Gln Asn Phe Asp Arg Phe Asp 945 950 955 960 Asn5 17 DNA Homo sapiens 5 ctggcccagt gagaacc 17 6 17 DNA Homo sapiens 6cagatgaacg ggaaacc 17 7 31 DNA Homo sapiens 7 atgggcttga gaaaatccccaggatcactt c 31 8 31 DNA Homo sapiens 8 catcagtgag accgacttcc gccgattccag 31 9 31 DNA Homo sapiens 9 tcaggagtgt gacaaaagat gtaagcatct t 31 10 31DNA Homo sapiens 10 cctctgcacc cagcctgtta gcatgaaccc t 31 11 31 DNA Homosapiens 11 gagagccgag cacccactga ctgacagcct t 31 12 17 DNA Homo sapiens12 gaaatggacc gtgcgtt 17 13 17 DNA Homo sapiens 13 agaccgtgag cctggat 17

What is claimed is:
 1. A method for identifying a subject as a candidatefor a particular clinical course of therapy to treat a vascular diseaseor disorder comprising the steps of: a) determining the identity of oneor more of the nucleotides present at nucleotide positions 55322, 53502,60793, 58445, 52861, and/or 49556 of SEQ ID NO:1, and/or the identity ofthe nucleotide present at nucleotide position 105290, 118019, and/or89835 of SEQ ID NO:3, or the complement thereof; and b) identifying thesubject as a candidate for a particular clinical course of therapy basedon the identity of one or more of the nucleotides present at nucleotidepositions 55322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ IDNO:1, and/or the identity of the nucleotide present at nucleotideposition 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof.
 2. The method of claim 1, wherein determining the identity ofthe nucleotides is by obtaining a nucleic acid sample from the subject.3. The method of claim 1, wherein the clinical course of therapy is useof a medical device.
 4. The method of claim 1, wherein the clinicalcourse of therapy is use of a surgical procedure.
 5. The method of claim3, wherein said medical device is selected from the group consisting of:a defibrillator, a stent, a device used in coronary revascularization, apacemaker, and any combination thereof.
 6. The method of claim 3,wherein said medical device is used in combination with a modulator ofTHBS1 and/or THBS4 gene expression or THBS1 and/or THBS4 polypeptideactivity.
 7. The method of claim 4, wherein said surgical procedure isselected from the group consisting of: percutaneous transluminalcoronary angioplasty, laser angioplasty, implantation of a stent,coronary bypass grafting, implantation of a defibrillator, implantationof a pacemaker, and any combination thereof.
 8. A method for identifyinga subject who is a candidate for further diagnostic evaluation for avascular disease or disorder comprising the steps of: a) determining theidentity of one or more of the nucleotides present at nucleotidepositions 55322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ IDNO:1, and/or the identity of the nucleotide present at nucleotideposition 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof; and b) identifying the subject as a subject who is a candidatefor further diagnostic evaluation for a vascular disease or disorderbased on the identity of one or more of the nucleotides present atnucleotide positions 55322, 53502, 60793, 58445, 52861, and/or 49556 ofSEQ ID NO:1, and/or the identity of the nucleotide present at nucleotideposition 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof, or the complement thereof.
 9. The method of claim 8, whereindetermining the identity of the nucleotides is by obtaining a nucleicacid sample from the subject.
 10. The method of claim 8, wherein saidfurther diagnostic evaluation consists of use of one or more vascularimaging devices.
 11. The method of claim 10, wherein said vascularimaging device is selected from the group consisting of: angiography,cardiac ultrasound, coronary angiogram, magnetic resonance imagery,nuclear imaging, CT scan, myocardial perfusion imagery,electrocardiogram, and any combination thereof.
 12. The method of claim8, wherein further diagnostic evaluation is selected from the groupconsisting of: genetic analysis, familial health history analysis,lifestyle analysis, exercise stress tests, and any combination thereof.13. A method for selecting a clinical course of therapy to treat asubject who is at risk for developing a vascular disease or disordercomprising the steps of: a) determining the identity of one or more ofthe nucleotides present at nucleotide positions 55322, 53502, 60793,58445, 52861, and/or 49556 of SEQ ID NO:1, and/or the identity of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof, and b) selecting a clinicalcourse of therapy for treatment of a subject who is at risk fordeveloping a vascular disease or disorder based on the identity of oneor more of the nucleotides present at nucleotide positions 55322, 53502,60793, 58445, 52861, and/or 49556 of SEQ ID NO:1, and/or the identity ofthe nucleotide present at nucleotide position 105290, 118019, and/or89835 of SEQ ID NO:3, or the complement thereof.
 14. The method of claim13, wherein determining the identity of the nucleotides is by obtaininga nucleic acid sample from the subject.
 15. The method of claim 13,wherein the clinical course of therapy comprises use of a medical devicefor treating a vascular disease or disorder.
 16. The method of claim 15,wherein said medical device is selected from the group consisting of: adefibrillator, a stent, a device used in coronary revascularization, apacemaker, and any combination thereof.
 17. The method of claim 15,wherein said medical device is used in combination with a modulator ofmodulators of THBS1 and/or THBS4 gene expression or THBS1 and/or THBS4polypeptide activity.
 18. The method of claim 13, wherein said clinicalcourse of therapy is use of a surgical procedure.
 19. The method ofclaim 18, wherein said surgical procedure is selected from the groupconsisting of: percutaneous transluminal coronary angioplasty, laserangioplasty, implantation of a stent, coronary bypass grafting,implantation of a defibrillator, implantation of a pacemaker, and anycombination thereof.
 20. A method for determining whether a subject willbenefit from implantation of a stent comprising the steps of: a)determining the identity of one or more of the nucleotides present atnucleotide positions 55322, 53502, 60793, 58445, 52861, and/or 49556 ofSEQ ID NO:1, and/or the identity of the nucleotide present at nucleotideposition 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof; and b) determining whether a subject will benefit fromimplantation of a stent based on the identity of one or more of thenucleotides present at nucleotide positions 55322, 53502, 60793, 58445,52861, and/or 49556 of SEQ ID NO:1, and/or the identity of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof.
 21. The method of claim 20,wherein determining the identity of the nucleotides is by obtaining anucleic acid sample from the subject.
 22. A method for determiningwhether a subject will benefit from use of a vascular imaging procedurecomprising the steps of: a) determining the identity of one or more ofthe nucleotides present at nucleotide positions 55322, 53502, 60793,58445, 52861, and/or 49556 of SEQ ID NO:1, and/or the identity of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof; and b) determining whether asubject will benefit from use of a vascular imaging procedure based onthe identity of one or more of the nucleotides present at nucleotidepositions 55322, 53502, 60793, 58445, 52861, and/or 49556 of SEQ IDNO:1, and/or the identity of the nucleotide present at nucleotideposition 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof.
 23. The method of claim 22, wherein determining the identity ofthe nucleotides is by obtaining a nucleic acid sample from the subject.24. The method of claim 22, wherein said vascular imaging procedure isselected from the group consisting of angiography, cardiac ultrasound,coronary angiogram, magnetic resonance imagery, nuclear imaging, CTscan, myocardial perfusion imagery, electrocardiogram, and anycombination thereof.
 25. A method for determining whether a subject willbenefit from a surgical procedure comprising the steps of: a)determining the identity of one or more of the nucleotides present atnucleotide positions 55322, 53502, 60793, 58445, 52861, and/or 49556 ofSEQ ID NO:1, and/or the identity of the nucleotide present at nucleotideposition 105290, 118019, and/or 89835 of SEQ ID NO:3, or the complementthereof; and b) determining whether a subject will benefit from asurgical procedure based on the identity of one or more of thenucleotides present at nucleotide positions 55322, 53502, 60793, 58445,52861, and/or 49556 of SEQ ID NO:1, and/or the identity of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof.
 26. The method of claim 25,wherein determining the identity of the nucleotides is by obtaining anucleic acid sample from the subject.
 27. The method of claim 25,wherein said surgical procedure is selected from the group consisting ofpercutaneous transluminal coronary angioplasty, laser angioplasty,implantation of a stent, coronary bypass grafting, implantation of adefibrillator, implantation of a pacemaker, and any combination thereof.28. A method for selecting an effective vascular imaging device as adiagnostic tool in a subject comprising the steps of: a) the identity ofone or more of the nucleotides present at nucleotide positions 55322,53502, 60793, 58445, 52861, and/or 49556 of SEQ ID NO:1, and/or theidentity of the nucleotide present at nucleotide position 105290,118019, and/or 89835 of SEQ ID NO:3, or the complement thereof, and b)selecting an effective vascular imaging device as a diagnostic tool forsaid subject based on the identity of one or more of the nucleotidespresent at nucleotide positions 55322, 53502, 60793, 58445, 52861,and/or 49556 of SEQ ID NO:1, and/or the identity of the nucleotidepresent at nucleotide position 105290, 118019, and/or 89835 of SEQ IDNO:3, or the complement thereof.
 29. The method of claim 28, whereindetermining the identity of the nucleotides is by obtaining a nucleicacid sample from the subject.
 30. The method of claim 28, wherein saidvascular imaging device is selected from the group consisting of:angiography, cardiac ultrasound, coronary angiogram, magnetic resonanceimagery, nuclear imaging, CT scan, myocardial perfusion imagery,electrocardiogram, and any combination thereof.
 31. A computer readablemedium for storing instructions for performing a computer implementedmethod for determining whether or not a subject has a predisposition toa vascular disease or disorder, said instructions comprising thefunctionality of: obtaining information from the subject indicative ofthe presence or absence of the polymorphic region of a THBS2, ACE,and/or a FGB gene, and based on the presence or absence of thepolymorphic region of a THBS2, ACE, and/or a FGB gene, determiningwhether or not the subject has a predisposition to a vascular disease ordisorder.
 32. A computer readable medium for storing instructions forperforming a computer implemented method for identifying apredisposition to a vascular disease or disorder, said instructionscomprising the functionality of: obtaining information regarding thepresence or absence of the polymorphic region of a THBS2, ACE, and/or aFGB gene, and based on the presence or absence of the polymorphic regionof a THBS2, ACE, and/or a FGB gene, identifying a predisposition to avascular disease or disorder.
 33. An electronic system comprising aprocessor for determining whether or not a subject has a predispositionto a vascular disease or disorder, said processor implementing thefunctionality of: obtaining information from the subject indicative ofthe presence or absence of the polymorphic region of a THBS2, ACE,and/or a FGB gene, and based on the presence or absence of thepolymorphic region of a THBS2, ACE, and/or a FGB gene, determiningwhether or not the subject has the predisposition to a vascular diseaseor disorder.
 34. An electronic system comprising a processor forperforming a method for identifying a predisposition to a vasculardisease or disorder in a subject, said processor implementing thefunctionality of: obtaining information from the subject indicative ofthe presence or absence of the polymorphic region of a THBS2, ACE,and/or a FGB gene, and based on the presence or absence of thepolymorphic region of a THBS2, ACE, and/or a FGB gene, performing amethod for identifying a predisposition to a vascular disease ordisorder associated with the polymorphic region.
 35. The electronicsystem of claims 33 or 34, wherein said processor further implements thefunctionality of receiving phenotypic information associated with thesubject.
 36. The electronic system of claims 33 or 34, wherein saidprocessor further implements the functionality of acquiring from anetwork phenotypic information associated with the subject.
 37. Anetwork system for identifying a predisposition to a vascular disease ordisorder in response to information submitted by an individual, saidsystem comprising means for: receiving data from the individualregarding the presence or absence of the polymorphic region of a THBS2,ACE, and/or a FGB gene, and based on the presence or absence of thepolymorphic region, determining whether or not the subject has thepredisposition to the vascular disease or disorder associated with thepolymorphic region.
 38. A network system for identifying whether or nota subject has a predisposition to a vascular disease or disorder, saidsystem comprising means for: receiving information from the subjectregarding the polymorphic region of a THBS2, ACE, and/or a FGB gene,receiving phenotypic information associated with the subject, acquiringadditional information from the network, and based on one or more of thephenotypic information, the polymorphic region, and the acquiredinformation, determining whether or not the subject has apre-disposition to a vascular disease or disorder associated with apolymorphic region of a THBS2, ACE, and/or a FGB gene.
 39. The system ofclaims 37 and 38, wherein the network system comprises a server and awork station operatively connected to said server via the network.
 40. Amethod for diagnosing or aiding in the diagnosis of a vascular diseaseor disorder in a subject comprising the steps of determining the THBS1and/or THBS4 genetic profile of the subject, thereby diagnosing oraiding in the diagnosis of a vascular disease or disorder.
 41. Themethod of claim 40, wherein determining the subject's THBS1 and/or THBS4genetic profile comprises determining the identity of one or more of thenucleotides present at nucleotide positions 55322, 53502, 60793, 58445,52861, and/or 49556 of SEQ ID NO:1, and/or the identity of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof.
 42. The method of claim 40,further comprising utilizing a vascular imaging device to diagnose oraid in the diagnosis of a vascular disease or disorder.
 43. The methodof claim 42, wherein the vascular imaging device is selected from thegroup consisting of: angiography, cardiac ultrasound, coronaryangiogram, magnetic resonance imagery, nuclear imaging, CT scan,myocardial perfusion imagery, electrocardiogram, and any combinationthereof.
 44. A method for selecting the appropriate drug to administerto a subject who has, or is at risk of developing, a vascular disease ordisorder, comprising determining the molecular structure of at least aportion of a THBS1 and/or a THBS4 gene of the subject.
 45. The method ofclaim 44, wherein determining the molecular structure comprisesdetermining the identity of the allelic variant of at least onepolymorphic region of the THBS1 and/or THBS4 gene of the subject. 46.The method of claim 44, wherein determining the molecular structurecomprises determining the identity of the allelic variant of at leastone polymorphic region of the THBS1 and/or THBS4 gene of the subject.47. A method for treating a subject having a disease or conditionassociated with a specific allelic variant of a polymorphic region of aTHBS1 and/or a THBS4 gene, comprising the steps of: (a) determining theidentity of a THBS1 and/or a THBS4 allelic variant; and (b)administering to the subject a compound that modulates THBS1and/or THBS4gene expression or protein activity.
 48. The method of claim 47, whereinthe specific allelic variant comprises a nucleotide sequence selectedfrom the group consisting of those set forth in one or more of SEQ IDNO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10,SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13, or the complements thereof.49. A method of diagnosing or aiding in the diagnosis of a vasculardisease in a subject comprising determining the nucleotide at nucleotideposition 55322, 53502, 60793, and/or 58445 of SEQ ID NO:1, and/or theidentity of the nucleotide present at nucleotide position 105290,118019, and/or 89835 of SEQ ID NO:3, or the complement thereof, whereinthe presence of at least one variant allele, or the complement thereof,is indicative of increased likelihood of a vascular disease in thesubject as compared with a subject having the reference allele at one ormore of these loci.
 50. The method of claim 49, wherein determining theidentity of the nucleotides is by obtaining a nucleic acid sample fromthe subject.
 51. A method of diagnosing or aiding in the diagnosis of avascular disease in a subject comprising determining the nucleotide atnucleotide position 52861 and/or 49556 of SEQ ID NO:1, or the complementthereof, wherein the presence of at least one variant allele, or thecomplement thereof, is indicative of decreased likelihood of a vasculardisease in the subject as compared with a subject having the referenceallele at one or more of these loci.
 52. The method of claim 51, whereindetermining the identity of the nucleotides is by obtaining a nucleicacid sample from the subject.
 53. The method of claims 49 or 51, whereinthe vascular disease is selected from the group consisting ofatherosclerosis, coronary artery disease, myocardial infarction,ischemia, stroke, peripheral vascular diseases, venous thromboembolismand pulmonary embolism.
 54. The method of claim 53, wherein the vasculardisease is myocardial infarction.
 55. The method of claim 53, whereinthe vascular disease is coronary artery disease.
 56. A method forpredicting the likelihood that a subject will have a vascular disease,comprising determining the nucleotide at nucleotide position 55322,53502, 60793, and/or 58445 of SEQ ID NO:1, and/or the identity of thenucleotide present at nucleotide position 105290, 118019, and/or 89835of SEQ ID NO:3, or the complement thereof, wherein the presence of atleast one variant allele, or the complements thereof, is indicative ofincreased likelihood of a vascular disease in the subject as comparedwith a subject having the reference allele at one or more of these loci.57. The method of claim 56, wherein determining the identity of thenucleotides is by obtaining a nucleic acid sample from the subject. 58.A method for predicting the likelihood that a subject will have avascular disease, comprising determining the nucleotide at nucleotideposition 52861 and/or 49556 of SEQ ID NO:1, or the complement thereof,wherein the presence of at least one variant allele, or the complementsthereof, is indicative of increased likelihood of a vascular disease inthe subject as compared with a subject having the reference allele atone or more of these loci.
 59. The method of claim 58, whereindetermining the identity of the nucleotides is by obtaining a nucleicacid sample from the subject.
 60. The method of claim 56 or 58, whereinthe vascular disease is selected from the group consisting ofatherosclerosis, coronary artery disease, myocardial infarction,ischemia, stroke, peripheral vascular diseases, venous thromboembolismand pulmonary embolism.
 61. The method of claim 60, wherein the vasculardisease is myocardial infarction.
 62. The method of claim 60, whereinthe vascular disease is coronary artery disease.
 63. An isolated nucleicacid molecule comprising a nucleotide sequence comprising an allelicvariant of a polymorphic region of a THBS1 gene, and allelic variants inlinkage disequilibrium therewith, or the complement thereof, wherein theallelic variant differs from the reference sequence set forth in SEQ IDNO:1, and wherein the allelic variant is associated with vasculardisease.
 64. An isolated nucleic acid molecule comprising a nucleotidesequence comprising an allelic variant of a polymorphic region of aTHBS4 gene, and allelic variants in linkage disequilibrium therewith, orthe complement thereof, wherein the allelic variant differs from thereference sequence set forth in SEQ ID NO:3, and wherein the allelicvariant is associated with vascular disease.
 65. A kit comprising probesor primers which are capable of hybridizing to the nucleic acid moleculeof one of claims 63 and
 64. 66. The kit of claim 65, wherein the probesor primers comprise a nucleotide sequence from about 15 to about 30nucleotides.
 67. The kit of claim 66, wherein the probes or primers arelabeled.
 68. A method for determining the identity of one or moreallelic variants of a polymorphic region of a THBS1 and/or THBS4 gene ina nucleic acid obtained from a subject, comprising contacting a samplenucleic acid from the subject with probes or primers having sequenceswhich are complementary to a THBS1 and/or THBS4, wherein the samplecomprises a THBS1 and/or THBS4 gene sequence, thereby determining theidentity of one or more of the allelic variants.
 69. The method of claim68, wherein the probes or primers are capable of hybridizing to anallelic variant of a polymorphic region, and wherein the allelic variantdiffers from the reference sequence set forth in SEQ ID NO:1 or SEQ IDNO:
 3. 70. The method of claim 68, wherein determining the identity ofthe allelic variant comprises determining the identity of at least onenucleotide of the polymorphic region of a THBS1 gene and/or a THBS4gene.
 71. The method of claim 68, wherein determining the identity ofthe allelic variant consists of determining the nucleotide content ofthe polymorphic region.
 72. The method of claim 68, wherein determiningthe nucleotide content comprises sequencing the nucleotide sequence. 73.The method of claim 68, wherein determining the identity of the allelicvariant comprises performing a restriction enzyme site analysis.
 74. Themethod of claim 68, wherein determining the identity of the allelicvariant is carried out by single-stranded conformation polymorphism. 75.The method of claim 68, wherein determining the identity of the allelicvariant is carried out by allele specific hybridization.
 76. The methodof claim 68, wherein determining the identity of the allelic variant iscarried out by primer specific extension.
 77. The method of claim 68,wherein determining the identity of the allelic variant is carried outby an oligonucleotide ligation assay.
 78. The method of claim 68,wherein the probe or primer comprises a nucleotide sequence from about15 to about 30 nucleotides.
 79. An Internet-based method for assessing asubject's risk for vascular disease, the method comprising: a) analyzingbiological information from a subject indicative of the presence orabsence of a polymorphic region of THBS1 and/or THBS4; b) providingresults of the analysis to the subject via the Internet, wherein thepresence of a polymorphic region of THBS1 and/or THBS4 indicates anincreased risk for vascular disease.
 80. A method of assessing asubject's risk for vascular disease, the method comprising: a) obtainingbiological information from the individual; b) analyzing the informationto obtain the subject's THBS1 and/or THBS4 genetic profile; c)representing the THBS1 and/or THBS4 genetic profile information asdigital genetic profile data; d) electronically processing the THBS1and/or THBS4 digital genetic profile data to generate a risk assessmentreport for vascular disease, wherein the presence of a polymorphicregion of THBS1 and/or THBS4 indicates an increased risk for vasculardisease; and e) displaying the risk assessment report on an outputdevice.
 81. A method of assessing a subject's risk for vascular disease,the method comprising: a) obtaining the subject's THBS1 and/or THBS4genetic profile information as digital genetic profile data; b)electronically processing the THBS1 and/or THBS4 digital genetic profiledata to generate a risk assessment report for vascular disease, whereinthe presence of a polymorphic region of THBS1 and/or THBS4 indicates anincreased risk for vascular disease; and c) displaying the riskassessment report on an output device.
 82. The method of claims 80 or81, further comprising the step of using the risk assessment report toprovide medical advice.
 83. The method of claims 80 or 81, whereinadditional health information is provided.
 84. The method of claim 83,wherein the additional health information comprises informationregarding one or more of age, sex, ethnic origin, diet, sibling health,parental health, clinical symptoms, personal health history, blood testdata, weight, and alcohol use, drug use, nicotine use, and bloodpressure.
 85. The method of claim 81, wherein the THBS1 and/or THBS4digital genetic profile data are transmitted via a communicationsnetwork to a medical information system for processing.
 86. The methodof claim 85, wherein the communications network is the Internet.
 87. Amedical information system for assessing a subject's risk for vasculardisease comprising: a) means for obtaining biological information fromthe individual to obtain a THBS1 and/or THBS4 genetic profile; b) meansfor representing the THBS1 and/or THBS4 genetic profile as digitalmolecular data; c) means for electronically processing the THBS1 and/orTHBS4 digital genetic profile to generate a risk assessment report forvascular disease; and d) means for displaying the risk assessment reporton an output device, wherein the presence of a polymorphic region ofTHBS1 and/or THBS4 indicates an increased risk for vascular disease. 88.A medical information system for assessing a subject's risk for vasculardisease comprising: a) means for representing the subject's THBS1 and/orTHBS4 genetic profile data as digital molecular data; b) means forelectronically processing the THBS1 and/or THBS4 digital genetic profileto generate a risk assessment report for vascular disease; and c) meansfor displaying the risk assessment report on an output device, whereinthe presence of a polymorphic region of THBS1 and/or THBS4 indicates anincreased risk for vascular disease.
 89. A computerized method ofproviding medical advice to a subject comprising: a) analyzingbiological information from a subject to determine the subject's THBS1and/or THBS4 genetic profile; b) based on the subject's THBS1 and/orTHBS4 genetic profile, determining the subject's risk for vasculardisease; c) based on the subject's risk for vascular disease,electronically providing medical advice to the subject.
 90. Acomputerized method of providing medical advice to a subject comprising:a) based on the subject's THBS1 and/or THBS4 genetic profile,determining the subject's risk for vascular disease; b) based on thesubject's risk for vascular disease, electronically providing medicaladvice to the subject.
 91. The method of any of claims 89 or 90, whereinthe medical advice comprises one or more of the group consisting offurther diagnostic evaluation, administration of medication, orlifestyle change.
 92. The method of claims 89 or 90, wherein additionalhealth information is obtained from the subject.
 93. The method of claim92, wherein the additional health information comprises informationregarding one or more of age, sex, ethnic origin, diet, sibling health,parental health, clinical symptoms, personal health history, blood testdata, weight, and alcohol use, drug use, nicotine use, and bloodpressure.
 94. A method for self-assessing risk for a vascular diseasecomprising a) providing biological information for genetic analysis; b)accessing an electronic output device displaying results of the geneticanalysis, thereby self-assessing risk for a vascular disease, whereinthe presence of a polymorphic region of THBS1 and/or THBS4 indicates anincreased risk for vascular disease.
 95. A method for self-assessingrisk for a vascular disease comprising accessing an electronic outputdevice displaying results of a genetic analysis of a biological sample,wherein the presence of a polymorphic region of THBS1 and/or THBS4indicates an increased risk for vascular disease, thereby self-assessingrisk for a vascular disease.
 96. A method of self-assessing risk forvascular disease, the method comprising a) providing biologicalinformation; b) accessing THBS1 and/or THBS4 digital genetic profiledata obtained from the biological information, the THBS1 and/or THBS4digital genetic profile data being displayed via an output device,wherein the presence of a polymorphic region of THBS1 and/or THBS4indicates an increased risk for vascular disease.
 97. A method ofself-assessing risk for vascular disease, the method comprisingaccessing THBS1 and/or THBS4 digital genetic profile data obtained frombiological information, the THBS1 and/or THBS4 digital genetic profiledata being displayed via an output device, wherein the presence of apolymorphic region of THBS1 and/or THBS4 indicates an increased risk forvascular disease.
 98. The method of claims 96 or 97, wherein theelectronic output device is accessed via the Internet.
 99. The method ofclaims 96 or 97, wherein additional health information is provided. 100.The method of claim 99, wherein the additional health informationcomprises information regarding one or more of age, sex, ethnic origin,diet, sibling health, parental health, clinical symptoms, personalhealth history, blood test data, weight, and alcohol use, drug use,nicotine use, and blood pressure.
 101. The method of any of claims 94,95, 96, or 97, wherein the biological information is obtained from asample from an individual at a laboratory company.
 102. The method ofclaim 101, wherein the laboratory company processes the biologicalsample to obtain THBS1 and/or THBS4 genetic profile data, represents atleast some of the THBS1 and/or THBS4 genetic profile data as digitalgenetic profile data, and transmits the THBS1 and/or THBS4 digitalgenetic profile data via a communications network to a medicalinformation system for processing.
 103. The method of any of claims 94,95, 96, or 97, wherein the biological information is obtained from asample from an individual at a draw station, wherein the draw stationprocesses the biological sample to obtain THBS1 and/or THBS4 geneticprofile data, and transfers the data to a laboratory company.
 104. Themethod of claim 103, wherein the laboratory company represents at leastsome of the THBS1 and/or THBS4 genetic profile data as digital geneticprofile data, and transmits the THBS1 and/or THBS4 digital geneticprofile data via a communications network to a medical informationsystem for processing.
 105. A method for a health care provider togenerate a personal health assessment report for an individual, themethod comprising counseling the individual to provide a biologicalsample; authorizing a draw station to take a biological sample from theindividual and transmit molecular information from the sample to alaboratory company, wherein the molecular information comprises thepresence or absence of a polymorphic region of THBS1 and/or THBS4;requesting the laboratory company to provide digital molecular datacorresponding to the molecular information to a medical informationsystem to electronically process the digital molecular data and digitalhealth data obtained from the individual to generate a health assessmentreport; receiving the health assessment report from the medicalinformation system; and providing the health assessment report to theindividual.
 106. A method for a health care provider to generate apersonal health assessment report for an individual, the methodcomprising requesting a laboratory company to provide digital moleculardata corresponding to the molecular information derived from abiological sample from the individual to a medical information system toelectronically process the digital molecular data and digital healthdata obtained to generate a health assessment report; receiving thehealth assessment report from the medical information system; andproviding the health assessment report to the individual.
 107. A methodof assessing the health of an individual, the method comprising:obtaining health information from the individual using an input device;representing at least some of the health information as digital healthdata; obtaining biological information from the individual, wherein theinformation comprises the presence or absence of a polymorphic region ofTHBS1 and/or THBS4; representing at least some of the information asdigital molecular data; electronically processing the digital moleculardata and digital health data to generate a health assessment report; anddisplaying the health assessment report on an output device.
 108. Themethod of claim 107, wherein electronically processing the digitalmolecular data and digital health data to generate a health assessmentreport comprises using the digital molecular data and digital healthdata as inputs for an algorithm or a rule-based system that determineswhether the individual is at risk for a specific disorder.
 109. Themethod of claim 107, wherein the individual has or is at risk ofdeveloping vascular disease, and wherein electronically processing thedigital molecular data and digital health data to generate a healthassessment report comprises using the digital molecular data and digitalhealth data as inputs for an algorithm or a rule-based system thatdetermines the individual's prognosis.
 110. The method of claim 107,wherein electronically processing the digital molecular data and digitalhealth data comprises using the digital molecular data and digitalhealth data as inputs for an algorithm or a rule-based system based onone or more databases comprising stored digital molecular data and/ordigital health data relating to one or more disorders.
 111. The methodof claim 107, wherein electronically processing the digital moleculardata and digital health data comprises using the digital molecular dataand digital health data as inputs for an algorithm or a rule-basedsystem based on one or more databases comprising (i) stored digitalmolecular data and/or digital health data from a plurality of healthyindividuals, and (ii) stored digital molecular data and/or digitalhealth data from one or more pluralities of unhealthy individuals, eachplurality of individuals having a specific disorder.
 112. The method ofeither of claims 110 or 111, wherein at least one of the databases is apublic database.
 113. The method of claim 107, wherein the digitalhealth data and digital molecular data are transmitted via acommunications network to a medical information system for processing.114. The method of claim 113, wherein the communications network is theInternet.
 115. The method of claim 113, wherein the input device is akeyboard, touch screen, hand-held device, telephone, wireless inputdevice, or interactive page on a website.
 116. The method of claim 107,wherein the health assessment report comprises a digital molecularprofile of the individual.
 117. The method of claim 107, wherein thehealth assessment report comprises a digital health profile of theindividual.
 118. The method of claim 107, wherein the molecular datacomprises nucleic acid sequence data, and the molecular profilecomprises a genetic profile.
 119. The method of claim 107, wherein themolecular data comprises protein sequence data, and the molecularprofile comprises a proteomic profile.
 120. The method of claim 107,wherein the molecular data comprises information regarding one or moreof the absence, presence, or level, of one or more specific proteins,polypeptides, chemicals, cells, organisms, or compounds in theindividual's biological sample.
 121. The method of claim 107, whereinthe health information comprises information relating to one or more ofage, sex, ethnic origin, diet, sibling health, parental health, clinicalsymptoms, personal health history, blood test data, weight, and alcoholuse, drug use, nicotine use, and blood pressure.
 122. The method ofclaim 107, wherein the health information comprises current andhistorical health information.
 123. The method of claim 107, furthercomprising obtaining a second set of biological information at a timeafter obtaining the first set of biological information; processing thesecond set of biological information to obtain a second set ofinformation; representing at least some of the second set of informationas digital second molecular data; and processing the molecular data andsecond molecular data to generate a health assessment report.
 124. Themethod of claim 123, further comprising obtaining second healthinformation at a time after obtaining the health information;representing at least some of the second health information as digitalsecond health data and processing the molecular data, health data,second molecular data, and second health data to generate a healthassessment report.
 125. The method of claim 107, wherein the healthassessment report provides information about the individual'spredisposition for vascular disease and options for risk reduction. 126.The method of claim 125, wherein the options for risk reduction compriseone or more of diet, exercise, one or more vitamins, one or more drugs,cessation of nicotine use, and cessation of alcohol use.
 127. The methodof claim 107, wherein the health assessment report provides informationabout treatment options for a particular disorder.
 128. The method ofclaim 127, wherein the treatment options comprise one or more of diet,one or more drugs, physical therapy, and surgery.
 129. The method ofclaim 107, wherein the health assessment report provides informationabout the efficacy of a particular treatment regimen and options fortherapy adjustment.
 130. The method of claim 107, further comprisingstoring the molecular data.
 131. The method of claim 130, furthercomprising building a database of stored molecular data from a pluralityof individuals.
 132. The method of claim 107, further comprising storingthe molecular data and health data.
 133. The method of claim 132,further comprising building a database of stored molecular data andhealth data from a plurality of individuals.
 134. The method of claim133, further comprising building a database of stored digital moleculardata and/or digital health data from a plurality of healthy individuals,and stored digital molecular data and/or digital health data from one ormore pluralities of unhealthy individuals, each plurality of individualshaving a specific disorder.